87value/wos11967 huggingface dataset:
A numerical analysis is presented of cell adhesion in capillaries whose diameter is comparable to or smaller than that of the cell. In contrast to a large number of previous efforts on leukocyte and tumor cell rolling, much is still unknown about cell motion in capillaries. The solid and fluid mechanics of a cell in flow was coupled with a slip bond model of ligand-receptor interactions. When the size of a capillary was reduced, the cell always transitioned to bullet-like motion, with a consequent decrease in the velocity of the cell. A state diagram was obtained for various values of capillary diameter and receptor density. We found that bullet motion enables firm adhesion of a cell to the capillary wall even for a weak ligand-receptor binding. We also quantified effects of various parameters, including the dissociation rate constant, the spring constant, and the reactive compliance on the characteristics of cell motion. Our results suggest that even under the interaction between P-selectin glycoprotein ligand-1 (PSGL-1) and P-selectin, which is mainly responsible for leukocyte rolling, a cell is able to show firm adhesion in a small capillary. These findings may help in understanding such phenomena as leukocyte plugging and cancer metastasis.
First impressions of social traits, such as trustworthiness or dominance, are reliably perceived in faces, and despite their questionable validity they can have considerable real-world consequences. We sought to uncover the information driving such judgments, using an attribute-based approach. Attributes (physical facial features) were objectively measured from feature positions and colors in a database of highly variable ambient face photographs, and then used as input for a neural network to model factor dimensions (approachability, youthful-attractiveness, and dominance) thought to underlie social attributions. A linear model based on this approach was able to account for 58% of the variance in raters' impressions of previously unseen faces, and factor-attribute correlations could be used to rank attributes by their importance to each factor. Reversing this process, neural networks were then used to predict facial attributes and corresponding image properties from specific combinations of factor scores. In this way, the factors driving social trait impressions could be visualized as a series of computer-generated cartoon face-like images, depicting how attributes change along each dimension. This study shows that despite enormous variation in ambient images of faces, a substantial proportion of the variance in first impressions can be accounted for through linear changes in objectively defined features.
In order to more fully understand the specific hydrodynamic relationship between young wind-generated gravity-capillary waves and longer gravity waves, a laboratory experiment was devised to observe changes in short wave spectral behavior over the phase of a long wave. This paper endeavors to expand on the body of laboratory wave modulation data and extend the investigation in support of the radar remote sensing of ocean surface waves. Measurements were made in the University of Miami's surge-structure-atmosphere interaction facility in the air-sea interaction saltwater tank wind-wave tank, with 10 m referenced wind speeds ranging between 5 and 23 m/s and paddle-generated wave steepnesses ak varying between 0.05 < ak < 0.3. A polarimetric camera was used to capture high sampling frequency maps of wave slope, yielding spatiotemporal information about short wind-wave behavior [provided as temporal variations in the wavenumber spectrum, where k approximate to (sic)(100-1000) rad/m]. The simultaneous and colocated long wave phase was measured via a side-looking camera. Hydrodynamic modulation transfer function (MTF) phases are found to be in general agreement with established values (between 2 and 10 radians) at the given wind speeds. The positive phase of the modulation places it immediately downwind of the long wave crest, with MTF magnitudes strongest for high wavenumbers at the lowest wind speeds. The results are also presented to show the modulation of gravity-capillary and pure capillary waves as variations in mean square slope over the long wave phase, with peak roughness enhancement found to move upwind of the long wave crest with increasing wind forcing.
Galactose metabolism in the yeast Saccharomyces cerevisiae is carried out by a specialized GAL pathway consisting of structural and regulatory proteins. It is known that cells with unbalanced Gal proteins accumulate toxic metabolic intermediates and exhibit severe growth defects. Recently, we found that the molecular chaperone Hsp90 controls the abundance of multiple Gal proteins, possibly to prevent these defects. Hsp90 regulates various cellular processes including cell morphology in response to environmental cues. Yeast cells are known to resort to filamentous growth upon exposure to galactose or other environmental stresses. Our previous and current findings support the Hsp90 titration model of Hsp90 buffering, which links the cell morphology and galactose pathways. Our results suggest that, when a large proportion of Hsp90 molecules are used to help Gal proteins, the Hsp90 client proteins in cell morphology pathways are left unattended, leading to filamentous growth. It remains unclear whether this phenomenon serves any biological function or simply reflects a cellular constraint. Nonetheless, it provides an alternative explanation why the GAL pathway is degenerated in some yeast species.
The primary aim was to determine the efficacy of the Distress Thermometer (DT) in screening for anxiety and mood problems against the University of Washington Quality of Life, version 4 (UWQOL). Secondary aims were to evaluate the association between demographic, clinical and health-related QOL variables with significant distress. Two hundred and sixty one disease-free HNC ambulatory patients attending routine follow-up clinics were prospectively recruited. Both DT and UWQOL were completed pre-consultation. Receiver operating characteristic (ROC) curve analyses of DT score for anxiety dysfunction yielded an area under the curve (AUC) of 0.877, with a sensitivity of 84% (43/51) and specificity of 76% (159/210) for a DT cut-off of ae4; with a corresponding AUC of 0.825 for mood with sensitivity 78% (28/36) and specificity 71% (159/225). Treatment with radiotherapy and a longer consultation time were associated with significant distress (DT ae4). Significant distress was also reported in two third of those reporting less than Good overall QOL. Distress levels were particularly associated with poor Social-Emotional function, more so than the association seen with poor physical function. DT is a reasonable screening tool for distress in the HNC population. The DT cut-off score ae4 was effective in identifying those with significant distress. Significant distress is associated in survivors with poor health-related quality of life, those who received radiotherapy and patients who have longer consultation times in clinic.
High-throughput screening (HTS) is currently the mainstay for the identification of chemical entities capable of modulating biochemical reactions or cellular processes. With the advancement of biotechnologies and the high translational potential of small molecules, a number of innovative approaches in drug discovery have evolved, which explains the resurgent interest in the use of HTS. The oncology field is currently the most active research area for drug screening, with no major breakthrough made for the identification of new immunomodulatory compounds targeting transplantation-related complications or autoimmune ailments. Here, we present a novel in vitro murine fluorescent-based lymphocyte assay easily adapted for the identification of new immunomodulatory compounds. This assay uses T or B cells derived from a transgenic mouse, in which the Nur77 promoter drives GFP expression upon T-or B-cell receptor stimulation. As the GFP intensity reflects the activation/ transcriptional activity of the target cell, our assay defines a novel tool to study the effect of given compound(s) on cellular/biological responses. For instance, a primary screening was performed using 4,398 compounds in the absence of a target hypothesis, which led to the identification of 160 potential hits displaying immunomodulatory activities. Thus, the use of this assay is suitable for drug discovery programs exploring large chemical libraries prior to further in vitro/in vivo validation studies.
At birth the piglet's immune system is immature and it is dependent upon passive maternal protection until weaning. The piglet's mucosal immune system develops over the first few weeks but has not reached maturity at weaning ages which are common on commercial farms. At weaning piglets are presented with a vast and diverse range of microbial and dietary/environmental antigens. Their ability to distinguish between antigens and mount a protective response to potential pathogens and to develop tolerance to dietary antigens is critical to their survival and failure to do so is reflected in the high incidence of morbidity and mortality in the post-weaning period. A growing recognition that the widespread use of antibiotics to control infection during this critical period should be controlled has led to detailed studies of those factors which drive the development of the mucosal immune system, the role of gut microbiota in driving this process, the origin of the bacteria that colonise the young piglet's intestine and the impact of rearing environment. This review briefly describes how the mucosal immune system is equipped to respond appropriately to antigenic challenge and the programmed sequence by which it develops. The results of studies on the critical interplay between the host immune system and gut microbiota are discussed along with the effects of rearing environment. By comparing these with results from human studies on the development of allergies in children, an approach to promote an earlier maturation of the piglet immune system to resist the challenges of weaning are outlined.
Background:Postdecompressive craniotomy defect management following failed prior cranioplastyis challenging. The authors describe a staged technique utilizing free muscle transfer, tissue expansion, and custom polyetheretherketone (PEEK) implants for the management of previously failed cranioplasty sites in patients with complicating local factors.Methods:Consecutive patients with previously failed cranioplasties following large decompressive craniectomies underwent reconstruction of skull and soft tissue defects with staged free latissimus muscle transfer, tissue expansion, and placement of custom computer-aided design and modeling PEEK implants with a temporalis-plus modification to minimize temporal hollowing. Implants were placed in a vascularized pocket at the third stage by elevating a plane between the previously transferred latissimus superficial fascia (left on the skin) and muscle (left on the dura/bone). Patients were evaluated postoperatively for cranioplasty durability, aesthetic outcome, and complications.Results:Six patients with an average of 1.6 previously failed cranioplasties underwent this staged technique. Average age was 33 years. Average defect size was 139 cm(2). Average time to procedure series completion was 14.9 months. There were no flap failures. One patient had early postoperative incisional dehiscence following PEEK implant placement that was managed by immediate scalp flap readvancement. At 21.9 month average follow-up, there were no cranioplasty failures. Three patients (50%) underwent 4 subsequent refining outpatient procedures. All patients achieved complete coverage of their craniectomy defect site with hear-bearing skin, acceptable head shape, and normalized head contour.Conclusions:The described technique resulted in aesthetic, durable craniectomy defect reconstruction with retention of native hear-bearing scalp skin in a challenging patient population.
Many patients with atopic syndrome complain of symptoms related to irritable bowel syndrome (IBS) as well. Symptoms caused by asthmatic condition or rhinitis are well known to the patients. There are well defined therapeutic strategies on how to handle the cases, well known to the patients. The cause of symptoms under IBS is unclear and many patients undergo many diagnostic procedures including invasive investigations. Quality of life (QOL) is reduced in such patients and they suffer a lot. Psychic disorders like depression and anxiety may occur. At the end of the Odyssey, food allergies are tested. Patients pay for laboratory tests for specific IgG4 antibodies or alternatively basophile activation tests (BAT) for food incompatibilities offered on online-platforms. In this article, causes of IBS through mast cell activation and not necessarily food allergies are discussed. How does mast cell activation lead to lactose, fructose and histamine intolerance? What are the similarities between Asthma and IBS? Is a similar therapeutic approach feasible?
As originally shown by King and Altman, graph theory, and specifically the use of spanning trees, provides the means to solve the kinetics of any catalytic network in a steady state regime, taking as input data all the rate constants. Herein, it is shown that the translation of the rate constants to Gibbs energies provides a simpler way to estimate the energy span (i.e., the apparent activation energy of the full reaction), the determining states, and the turnover frequency (TOF) of any and all catalytic networks. By re-examining the concepts of chemical kinetics through rigorous mathematical treatment, an alternative definition is suggested for the term chemical mechanism. In addition, and in analogy to electrical circuits, the chemical resistor terms (called here kinestors) are identified for parallel and series chemical circuits, providing a new Ohmic interpretation for catalysis.
Authors often convey meaning by referring to or imitating prior works of literature, a process that creates complex networks of literary relationships (intertextuality) and contributes to cultural evolution. In this paper, we use techniques from stylometry and machine learning to address subjective literary critical questions about Latin literature, a corpus marked by an extraordinary concentration of intertextuality. Our work, which we term quantitative criticism, focuses on case studies involving two influential Roman authors, the playwright Seneca and the historian Livy. We find that four plays related to but distinct from Seneca's main writings are differentiated from the rest of the corpus by subtle but important stylistic features. We offer literary interpretations of the significance of these anomalies, providing quantitative data in support of hypotheses about the use of unusual formal features and the interplay between sound and meaning. The second part of the paper describes a machine-learning approach to the identification and analysis of citational material that Livy loosely appropriated from earlier sources. We extend our approach to map the stylistic topography of Latin prose, identifying the writings of Caesar and his near-contemporary Livy as an inflection point in the development of Latin prose style. In total, our results reflect the integration of computational and humanistic methods to investigate a diverse range of literary questions.
Within the diagnostic process, in the activities in which digital radiology systems are utilised, image display devices play a very important role. Such devices have to guarantee high performances in order to maintain the diagnostic quality of the displayed images and present them so that they can be observed optimally. In order to ensure the appropriate performance to the users, not only the image display device but also the whole workstation should be designed properly. Particular attention should be paid to the lighting conditions, to avoid compromising the radiologist's ability to observe the displayed images. The aim of this study is to assess the lighting of some workstations used for diagnostic radiology reporting, in order to obtain information about the visual interaction between worker and device and prevent health risks. A detailed analysis of the lighting of adjustable and nonadjustable workstations used for diagnostic radiology reporting was carried out in different rooms of the Radiology Department at the Felice Lotti Hospital in Pontedera (Pisa, Italy). The analysis was carried out thanks to in situ measurements (objective analysis) and the submission of an evaluation questionnaire to a sample composed by 16 radiologists (subjective analysis). From the obtained results it has been possible to point out that, in nonadjustable workstations the absence of a dedicated lighting system very often reveals the inadequacy of the general lighting in guaranteeing correct lighting conditions for the radiologists during the reading of the diagnostic monitors. As measure to reduce the visual fatigue of the radiologists, the use of a supplementary adjustable innovative LED backlight system was proposed. The use of the LED backlight system allows complying the requirements of the Technical Standards and mitigate the difference of luminance in the field of view of the radiologist. Due to that the use of the LED backlight system has been positively perceived by the examined sample, a significant part of whom has ascribed to this use a reduction in the visual fatigue. (C) 2016 Elsevier B.V. All rights reserved.
Background: Advances in the nucleic acid sequencing technologies have ushered in the era of genetic-based precision medicine. Applications of the genetic discoveries to practice of medicine, however, are hindered by phenotypic variability of the genetic variants. The report illustrates extreme pleiotropic phenotypes associated with an established causal mutation for hereditary cardiomyopathy. Case presentation: We report a 61-year old white female who presented with syncope and echocardiographic and cardiac magnetic resonance (CMR) imaging findings consistent with the diagnosis of hypertrophic cardiomyopathy (HCM). The electrocardiogram, however, showed a QRS pattern resembling an Epsilon wave, a feature of arrhythmogenic right ventricular cardiomyopathy (ARVC). Whole exome sequencing (mean depth of coverage of exons 178X) analysis did not identify a pathogenic variant in the known HCM genes but identified an established causal mutation for ARVC. The mutation involves a canonical splice accepter site (c.2146-1G >C) in the PKP2 gene, which encodes plakophillin 2. Sanger sequencing confirmed the mutation. PKP2 is the most common causal gene for ARVC but has not been implicated in HCM. Findings on echocardiography and CMR during the course of 4-year follow up showed septal hypertrophy and a hyperdynamic left ventricle, consistent with the diagnosis of HCM. However, neither baseline nor follow up echocardiography and CMR studies showed evidence of ARVC. The right ventricle was normal in size, thickness, and function and there was no evidence of fibro-fatty infiltration in the myocardium. Conclusions: The patient carries an established pathogenic mutation for ARVC and a subtle finding of ARVC but exhibits the classic phenotype of HCM, a contrasting phenotype to ARVC. The case illustrates the need for detailed phenotypic characterization for patients with hereditary cardiomyopathies as well as the challenges physicians face in applying the genetic discoveries in practicing genetic-based precision medicine.
Thyroid cancer is very common, but skull ectopic thyroid cancer has not been reported in 50 years of literatures in foreign countries. There are only four cases of the skull ectopic thyroid cancer reported in more than 30 years of domestic literature including the cases in this report. This paper aims to investigate the clinical characteristics and possible molecular mechanisms of this rare disease. Five keywords of thyroid gland, ectopic thyroid, thyroid cancer, ectopic thyroid cancer and metastatic thyroid cancer were included and 50 years of literatures in the PubMed-MEDLINE and Wanfang database were reviewed. By combining the test data of 2 cases of surgical patient tissue microarray specimens-molecular immunology pathology, the possible molecular mechanisms were analyzed and molecular regulation network diagram was drawn. The skull ectopic thyroid cancer has not been reported in 50 years of literatures in foreign countries and there are only four cases of the skull ectopic thyroid cancer reported in more than 30 years of domestic literature including the cases in this report. The molecular expressions of skull ectopic thyroid cancer, orthotopic thyroid cancer, and metastatic thyroid cancer were not the same: (I) AKT (P=0.012, 0.002) and mTOR (P=0.002, 0.004) were highly expressed in the skull ectopic thyroid cancer; (II) BRAF (P=0.029, 0.014) and ERK (P=0.002, 0.001) were highly expressed in orthotopic thyroid cancer; (III) MMP-9 (P=0.023, 0.016) was highly expressed in metastatic thyroid cancer. According to the molecular information base, the PI3K is predicted to be a key crossing gene of the above three signaling pathways, which showed no significant differences in these three thyroid cancers (P=0.692, 0.388, 0.227), but PI3K has regulation roles in the three signaling pathways of Akt/mTOR, MAPK, and NF-kappa B. PI3K gene is an important starting gene of thyroid cancers. After the canceration starts, due to the fact that the local microenvironments of thyroid cancers in different parts are different, the thyroid cancers are regulated by different signaling pathways. The ectopic thyroid cancer was correlated with Akt/mTOR pathway high expression; orthotopic thyroid was related with MAPK/BRAF/ERK signaling pathway high expression; and the metastatic thyroid cancer was related with NFkB/MMP9 high expression.
The occurrence of repetitive behaviours that are often harmful has been attributed to traits traditionally described as impulsive or compulsive e.g. substance dependence, excessive gambling, and hoarding. These behaviours are common and often co-occur in both the general population and psychiatric populations. The lack of measures to concurrently index a range of such behaviours led to the development of the Impulsive-Compulsive Behaviours (ICB) Checklist This study aims to validate the ICB Checklist in a general community sample. Factor analyses revealed a two-factor structure, demonstrating good model fit in two independent samples. These were labelled Impulsive-Compulsions and Compulsive-Impulsions, comprising of classically compulsive and impulsive behaviours respectively. Reliability and construct validity were further confirmed using correlations with existing Measures of impulsivity and compulsivity. Results suggest that the ICB Checklist is a valid and practical assessment that can be used to monitor behavioural clusters characterised by deficits in inhibition. (C) 2016 Elsevier Ltd. All rights reserved.