The effectiveness of double-gloving in otolaryngology. Thirty-four sets of consecutively used double gloves (68 pairs) used by otolaryngologists and their assistants in operations known to have a high risk of glove punctures were studied. The perforation rates for the outer and inner layers were 35.3 and 8.8% respectively, indicating that a second set of gloves substantially improves the likelihood of maintaining an intact barrier between medical staff and patient. 