Patent ID: 9708637
Date: 2017-07-18
CPC Classifications: C12N,C12P,C12Y,Y02P

Claim:
1. A method for producing a lower alkyl ester of α-L-aspartyl-L-phenylalanine, comprising: A) cultivating a bacterium of B) synthesizing the lower alkyl ester of α-L-aspartyl-L-phenylalanine from aspartic acid or a derivative thereof and the L-phenylalanine obtained in step A); wherein the bacterium comprises a DNA comprising: i) a gene selected from the group consisting of tyrA, pheA, pgi, ilvE, ilvA, tdcB, sdaA, sdaB, argA, argG, proB, thrB, and combinations thereof, and ii) a DNA fragment able to be transcribed and encoding the peptide of SEQ ID NO: 2, or a variant thereof consisting of a deletion, insertion, substitution, or addition of 1 amino acid as compared to SEQ ID NO: 2; and wherein said DNA fragment of ii) is attached to the 3′ end of said gene of i) and consequently enhances production of an L-amino acid.