Patent ID: 9701974
Date: 2017-07-11
CPC Classifications: C12N

Claim:
1. A nucleic acid molecule comprising a first DNA segment comprising a first transcription unit located between a first left T-DNA border region and a first right T-DNA border region, wherein at least one of said first left T-DNA border region and said first right T-DNA border region is flanked on both sides by and adjacent to a first and a second shorter transcribable nucleic acid sequence in opposite orientation to each other, wherein said first and second shorter transcribable nucleic acid sequences are homologous to a portion of a sequence of interest within said first transcription unit, and wherein said T-DNA border regions are capable of enabling the transfer of said segment into a genome; wherein each shorter transcribable nucleic acid sequence is at least 21 nucleotides long and comprises at least 21 contiguous nucleotides of the sequence of interest.