Patent ID: 9738924
Date: 2017-08-22
CPC Classifications: C12Q

Claim:
1. A method for quantitating nucleic acid hairpin molecules whose renaturations are blocked by single-stranded nucleic acid molecules comprising: a) providing a sample containing nucleic acid hairpin molecules, each of the nucleic acid hairpin molecules consisting of a double-stranded stem and a single-stranded loop, b) applying a force to the movable bead such that the movable bead is moved away from the fixed surface, thereby yielding completely denatured hairpin molecules; c) providing the single-stranded nucleic acid molecules complementary to the completely denatured hairpin molecules: d) reducing the force applied to the movable bead to allow renaturation of the completely denatured hairpin molecules in the presence of the single-stranded nucleic acid molecules; and e) detecting blockage of the renaturation of the hairpin molecules due to hybridization of the single-stranded nucleic acid molecules to the completely denatured hairpin molecules during step d); and f) quantitating the number of the nucleic acid hairpin molecules whose renaturations are blocked by the single-stranded nucleic acid molecules.