Patent ID: 9631031
Date: 2017-04-25
CPC Classifications: C07K

Claim:
1. An isolated multispecific antigens-binding fragment comprising at least two Fab fragments with different CH1 and CL domains, wherein said Fab fragments comprise: a. a wild-type Fab fragment consisting of wild-type human CH1 and wild-type human CL domains of an immunoglobulin, and the VH and VL domains of an antibody recognizing an epitope of interest; and b. a mutated Fab fragment consisting of: wherein the sequence position numbers used for the CH1 and CL domains refer to Kabat numbering and each Fab fragment recognizes a different epitope of interest, and said Fab fragments are tandemly arranged in any order, the C-terminal end of the CH1 domain of the first Fab fragment being linked to the N-terminal end of the VH domain of the following Fab fragment through a polypeptide linker.