Patent ID: 9645056
Date: 2017-05-09
CPC Classifications: G01N

Claim:
1. A method for immunohistochemical staining of a paraffin-embedded tissue sample, which comprises steps of: (a) contacting the paraffin-embedded tissue sample with a pretreatment solution of a pH of 5.0-10.0 at a first temperature range of 20 to 70° C. to promote deparaffinization and then the temperature is raised to a second temperature range of 80 to 130° C. to promote antigen retrieval to provide a deparaffinized and antigen-retrieved tissue sample; (b) washing the deparaffinized and antigen-retrieved tissue sample obtained in (a) with said pretreatment solution; and (c) immunohistochemical staining the washed tissue sample, wherein said pretreatment solution comprises a pH buffer, two nonionic surfactants, and cyclodextrin or a derivative thereof, with the balance being not less than 80 mass % of water; wherein said two nonionic surfactants are a polyoxyethylene alkyl phenyl ether nonionic surfactant and a polyoxyethylene sorbitan nonionic surfactant, a content of said polyoxyethylene alkyl phenyl ether nonionic surfactant being 0.01 to 1.0 mass %, and a content of said polyoxyethylene sorbitan nonionic surfactant being 0.3 to 3.0 mass %, wherein said pH buffer is selected from the group consisting of a citrate buffer, a citrate buffer mixed with a chelating agent, and a Tris buffer mixed with a chelating agent, and a content of said pH buffer being such that a pH of said pretreatment solution is 5.0 to 10.0; and wherein a content of said cyclodextrin or a derivative thereof is 0.01 to 1.0 mass %.