Patent ID: 9650608
Date: 2017-05-16
CPC Classifications: A61K,A61P,C12N

Claim:
1. A method of preparing an adipose-derived mesenchymal stem cell preparation for treating a bone, ligament, tendon or cartilage injury in an animal, comprising: a. collecting adipose tissue from a source animal, said adipose tissue including fat, connective tissue and mesenchymal stem cells; b. contacting said adipose tissue with an enzyme preparation comprising 55-65 wt % collagenase from c. isolating mesenchymal stem cells after step (b); d. incubating isolated mesenchymal stem cells from step c with a platelet rich plasma derived from the source animal to form a mixture; e. photoactivating the mixture from step d; and f. administering the mixture to the animal.