Patent ID: 9623149
Date: 2017-04-18
CPC Classifications: A61L

Claim:
1. A method for preparing an acellular dermal matrix comprising the steps of: i) removing epidermis of an isolated allogeneic skin; ii) removing cells in dermis of the epidermis-removed skin; iii) mixing a cryoprotectant to include glycerol, propylene glycol, sucrose and a basic solvent or solution, wherein the mixing ratio of glycerol, propylene glycol and the basic solvent or solution is 0.8-1.5:0.8-1.5:7-9 based on weight, and the cryoprotectant contains sucrose in an amount ranging from 25% to 40% by weight of the cryoprotectant; iv) penetrating the cryoprotectant into the skin from which epidermis and cells in dermis are removed; and v) freeze-drying the cryoprotectant-penetrated skin at a freezing rate of from −0.1 to −5° C. per minute to obtain an acellular dermal matrix.