Patent ID: 9663773
Date: 2017-05-30
CPC Classifications: C12N,C12Y,G16B

Claim:
1. A recombinant meso-active thermo-stable enzyme, wherein said recombinant enzyme is a cellulase, xylanase, amylase or a protease, wherein said recombinant enzyme comprises a mutated beta sheet-based substrate-binding and catalytically active surface, wherein said mutated active surface comprises a peptide sequence resulting from replacing, within a set of non-contiguous amino acid residues that comprise a beta sheet-based substrate-binding and catalytically active surface of a thermophile recipient enzyme, all the residues that are non-identical at structurally equivalent positions to a corresponding set of non-contiguous amino acid residues that comprise a beta sheet-based substrate-binding and catalytically active surface of a mesophile donor enzyme, wherein the residues in the active surface of the thermophile recipient enzyme that have been replaced, have been replaced with the residues at the structurally equivalent positions within the active surface of the mesophile donor enzyme, and wherein said recombinant enzyme comprises the structural stability characteristics of said thermophile enzyme, and the activity characteristics of said mesophile enzyme.