Patent ID: 9551703
Date: 2017-01-24
CPC Classifications: B01J,G01N

Claim:
1. A method for determining the abundance of one or more target analytes in a sample, the method comprising: a) contacting a target analyte with a set of detection units for each analyte fixed to a rectangular substrate in an array format, wherein each detection unit comprises a discrete amount of a capture agent capable of specifically binding the target analyte, and the amount of capturing agent varies over the set to form an exponential concentration gradient, under conditions that allow binding of the target analyte to the capture agent, wherein the set of detection units comprises multiple replicates for any single concentration level of any single capturing agent, such that the CV (coefficient of variation) is less than 4% for detection of the target analyte concentration in the sample, b) detecting binding of the target analyte to the capture agent, and c) determining the amount of target analyte present in the sample, wherein said step of determining comprises logarithmic transformation and curve fitting.