Patent ID: 9663581
Date: 2017-05-30
CPC Classifications: C07K

Claim:
1. A preparation comprising a glycoprotein comprising a modified amino acid sequence of a reference glycoprotein, the reference glycoprotein comprising an immunoglobulin Fc region or Fc fragment; wherein the glycoprotein of the preparation comprises an Fc region comprising two amino acid substitutions relative to the Fc region or Fc fragment of the reference glycoprotein, and wherein the two amino acid substitutions are selected from the group consisting of F241A/F243A, F241A/K246A, F241A/T260A, F241A/R301A, F243A/K246A, F243A/T260A, F243A/R301A, K246A/T260A, K246A/R301A, and T260A/R301A, as numbered according to the EU numbering index of Kabat, and wherein the preparation of the glycoprotein has an increased level of one or more of sialylated glycans, fucosylated glycans, and N-acetylglucosamine glycans, relative to a preparation of the reference glycoprotein.