Patent ID: 9580477
Date: 2017-02-28
CPC Classifications: A61K,C07K,C12N

Claim:
1. A recombinant DNA comprising a nucleic acid sequence encoding a recombinant protein, wherein the recombinant protein comprises a recombinant HIV-1 gp140 fused to a tag through a peptide linker at C-terminus of the recombinant HIV-1 gp140, wherein the peptide linker is sufficiently long so that the tag is accessible for binding by a binding molecule bound on a solid matrix, wherein the recombinant protein assembles into recombinant trimers that mimic native HIV-1 envelope trimers when the recombinant protein is expressed by cells carrying the nucleic acid sequence encoding the recombinant protein, and wherein the recombinant protein comprises SEQ ID NO: 10.