Patent ID: 9562071
Date: 2017-02-07
CPC Classifications: C07K

Claim:
1. A method for preparing eptifibatide, characterized in comprising: (a) obtaining a refined eptifibatide peptide solution; (b) rotarily evaporating the refined eptifibatide peptide solution, obtaining and freeze-drying a refined eptifibatide peptide concentrate; wherein the concentration of the refined eptifibatide peptide concentrate is 15-20 mg/mL; wherein the temperature of the rotary evaporation is 30° C.-35° C.; and wherein the refined eptifibatide peptide solution is prepared by obtaining a Cys-resin by coupling Cys to a resin; obtaining a polypeptide having a sequence as represented by SEQ ID NO: 1 by sequentially coupling the Cys-resin with Pro, Trp, Asp, Gly, Har and Mpa; and obtaining the refined eptifibatide peptide solution through oxidation, cleavage, purification and salt conversion.