Patent ID: 9671393
Date: 2017-06-06
CPC Classifications: C12N,G01N

Claim:
1. A plurality of isolated, heat-killed, chromosomal DNA interstrand-crosslinked, cell wall protein-crosslinked, protein A-positive Staphylococcus cells comprising a plurality of cell wall protein crosslinks and a plurality of chromosomal DNA interstrand crosslinks, wherein the protein A-positive Staphylococcus cells are heat-killed, each cell wall protein crosslink comprises a covalently-bound aldehyde crosslinker chosen from formaldehyde, paraformaldehyde, or glutaraldehyde, and each chromosomal DNA interstrand crosslink comprises a covalently-bound ultraviolet light-activated crosslinker chosen from psoralen, 8-methoxypsoralen, 4,5′,8-trimethylpsoralen, or 4′-aminomethyl-4,5′,8-trimethylpsoralen, wherein a plurality of DNA-protein complexes isolated using the plurality of isolated, heat-killed, chromosomal DNA interstrand-crosslinked, cell wall protein-crosslinked, protein A-positive Staphylococcus cells comprises Staphylococcus DNA that does not interfere with sequence-independent analysis of DNA in the plurality of DNA-protein complexes, and the plurality of DNA-protein complexes has reduced amplifiable Staphylococcus DNA as compared to the same DNA-protein complexes isolated using a plurality of heat-killed, cell wall protein-crosslinked, protein A-positive Staphylococcus cells that are devoid of chromosomal DNA interstrand crosslinks.