Patent ID: 9657345
Date: 2017-05-23
CPC Classifications: C12Q

Claim:
1. A method of producing a nucleotide sequence complementary to a linear target nucleic acid, the method comprising consisting essentially of: hybridizing a linear target nucleic acid with a dual-hybridization polynucleotide to form a hybridized complex of the linear target nucleic acid and the dual-hybridization polynucleotide, wherein the dual-hybridization polynucleotide comprises a first complementary region that is complementary to at least two consecutive nucleotides of the 3′-terminus of a linear target nucleic acid, wherein the length of the first complementary region in the dual hybridization polynucleotide is 2 to 4 nucleotides; a second complementary region that is complementary to at least two consecutive nucleotides from the 5′-terminus of a linear target nucleic acid, wherein the first complementary region is located 3′ of the second complementary region in the dual hybridization polynucleotide, wherein the second complementary region in the dual hybridization polynucleotide comprises nucleotides that are complementary to about 3 to 20 consecutive nucleotides from the 5′-terminal of the linear target nucleic acid; and a third region that is not complementary to the linear target nucleic acid and is located 5′ of the second complementary region, so that the second complementary region is positioned between the first complementary region and the third region; and incubating the hybridized complex of the linear target nucleic acid and dual hybridization polynucleotide in the presence of a nucleic acid polymerase to produce a nucleotide sequence complementary to the linear target nucleic acid by extending the 3′-terminal of the polynucleotide.