Patent ID: 9683997
Date: 2017-06-20
CPC Classifications: A61K,A61P,C07K,C12Q,G01N

Claim:
1. A method for measuring the antibody titer of a cross-neutralizing antibody against human papillomavirus (HPV), which comprises the steps of: (a) bringing a test sample into contact with an HPV antigen to establish binding of cross-neutralizing antibodies in the sample to the antigen; (b) adding a monoclonal antibody comprising the amino acid sequences shown in SEQ ID NO: 11 (CDRH1), SEQ ID NO: 12 (CDRH2), SEQ ID NO: 13 (CDRH3), SEQ ID NO: 14 (CDRL1), SEQ ID NO: 15 (CDRL2) and SEQ ID NO: 16 (CDRL3) or a monoclonal antibody comprising the amino acid sequences shown in SEQ ID NO: 17 (CDRH1), SEQ ID NO: 18 (CDRH2), SEQ ID NO: 19 (CDRH3), SEQ ID NO: 20 (CDRL1), SEQ ID NO: 21 (CDRL2) and SEQ ID NO: 22 (CDRL3) to the reaction system in step (a); (c) contacting the monoclonal antibody bound to the antigen with a labeled secondary antibody recognizing the monoclonal antibody to determine the amount of the monoclonal antibody bound to the antigen; and (d) measuring signal intensity arising from the labeled secondary antibody bound to the monoclonal antibody in the presence and absence of the test sample, thereby determining the antibody titer of the cross-neutralizing antibody in the test sample.