Patent ID: 9540452
Date: 2017-01-10
CPC Classifications: A01K,C07K,C12N

Claim:
1. A method for making an antibody comprising a human λ variable domain, the method comprising the steps of: (a) exposing a genetically modified mouse to an antigen, wherein the genetically modified mouse has a genome comprises an immunoglobulin locus comprising human Vλ and Jλ gene segments operably linked to a mouse Cκ gene such that the mouse expresses an immunoglobulin light chain that comprises a human λ variable domain derived from the Vλ and Jλ gene segments fused with a mouse κ constant domain; (b) allowing the genetically modified mouse to develop an immune response to the antigen; and (c) isolating from the mouse of (b) an antibody specific for the antigen, wherein the antibody comprises the human λ variable domain derived from the human Vλ and Jλ gene segments.