Patent ID: 9719089
Date: 2017-08-01
CPC Classifications: A61K,A61P,B82Y,C12N

Claim:
1. A method of inhibiting expression of a gene product comprising the step of hybridizing a polynucleotide encoding said gene product with one or more oligonucleotides complementary to all or a portion of said polynucleotide, said oligonucleotide being covalently bound to a nanoparticle that is from about 5 nanometers (nm) to about 50 nm in mean diameter, wherein the oligonucleotide comprises a spacer that creates a distance between the oligonucleotide and the nanoparticle that is equivalent to at least 10 nucleotides, wherein said nanoparticle has an in vitro property of inhibiting expression of said gene product by at least 5% compared to expression in the absence of the oligonucleotide and in the absence of a transfection agent, and wherein hybridization of the polynucleotide encoding said gene product and said oligonucleotide results in inhibiting expression of said gene product.