Patent ID: 9708639
Date: 2017-07-18
CPC Classifications: C12Q,G01N

Claim:
1. A method for quantifying an amino acid, which comprises: (A) reacting an aminoacyl-tRNA synthetase (AARS) corresponding to the amino acid with the amino acid and adenosine triphosphate (ATP) in the presence of an (aminoacyl-adenosine monophosphate (AMP))-AARS complex decomposition reagent to generate pyrophosphate, wherein the AARS is regenerated in free form via decomposition of a formed (aminoacyl-AMP)-AARS complex, (B) quantifying the pyrophosphate generated in (A) by a method comprising reacting pyrophosphate dikinase (PPDK) with the generated pyrophosphate in the presence of AMP and phosphoenolpyruvate (PEP) to generate ATP, phosphoric acid, and pyruvate, and (C) quantifying the generated pyruvate; wherein the amount of the amino acid is determined based on the amount of pyruvate, and wherein said method is performed in the absence of tRNA.