Patent ID: 9656263
Date: 2017-05-23
CPC Classifications: B01L,B03B,G01N

Claim:
1. A micro scaled method for separating a subgroup of cells and/or particles from a mixture of different types of cells and/or particles in a suspension, comprising the steps of: i) subjecting a suspension to pressure, wherein said pressure forces said suspension into one inlet and into at least one pre-alignment channel present on a microfluid chip; ii) subjecting said suspension to a two dimensional acoustic force directed perpendicular to the length direction of the pre-alignment channel, thereby forcing said mixture of cells and/or particles away from the walls, floor, and ceiling of the pre-alignment channel and confining said mixture of cells and/or particles into one or more discrete positions in the plane transverse to the length direction of the pre-alignment channel; iii) forcing said mixture of cells and/or particles and infusing cell free buffer solution through a second inlet simultaneously into at least one separation channel, wherein said mixture of cells and/or particles are forced towards the walls of the separation channel; iv) subjecting said cells and/or particles to a one dimensional acoustic force directed perpendicular to the length direction of the separation channel; and v) collecting cells and/or particles having the same size and/or mass density and/or compressibility from said separation channel present on the microfluid chip through at least two outlets, wherein the two dimensional acoustic force is actuated by a single transducer.