Patent ID: 9738912
Date: 2017-08-22
CPC Classifications: C12N,C12P,C12Y

Claim:
1. A recombinant fungal cell comprising: (a) at least one genetic modification in an endogenous gene with at least 80% nucleotide sequence identity to the polynucleotide of SEQ ID NO: 7, wherein the endogenous gene encodes a phosphatidylinositol transfer protein, and wherein the at least one genetic modification comprises (i) a deletion of at least a portion of the coding region of the endogenous gene, (ii) a mutation in the endogenous gene such that the gene encodes a polypeptide having reduced activity; (iii) a modified regulatory sequence that reduces expression of the endogenous gene; or (iv) any combination of (i)-(iii); and (b) an exogenous gene encoding a fatty acyl reductase (FAR) protein, wherein the exogenous gene is operably linked to a promoter, wherein the FAR protein has at least 80% amino acid sequence identity to a polypeptide selected from the group consisting of the polypeptides of SEQ ID NO: 2, 4, 6, and 65-73.