Patent ID: 9738928
Date: 2017-08-22
CPC Classifications: C12Q

Claim:
1. A method for detecting blockage of the renaturation of a hairpin molecule, said method comprising: a) providing a nucleic acid hairpin molecule consisting of a double-stranded stem and a single-stranded loop, wherein 5′ and 3′ ends of the nucleic acid hairpin molecule are bound to different surfaces; b) applying a force to one of the surfaces such that it is moved away from another of the surfaces, thereby yielding a completely denatured hairpin molecule; c) hybridizing a known single-stranded nucleic acid primer to the completely denatured hairpin molecule; d) extending the primer with a polymerase, thereby generating a nucleic acid molecule complementary to the completely denatured hairpin molecule; e) reducing the force applied to allow renaturation of the completely denatured hairpin molecule in the presence of the nucleic acid molecule complementary to the completely denatured hairpin molecule; and f) detecting blockage of the renaturation of the completely denatured hairpin molecule in step e) due to generation of the nucleic acid molecule complementary to the completely denatured hairpin molecule.