Patent ID: 9541751
Date: 2017-01-10
CPC Classifications: G01N,G02B,H04N

Claim:
1. A method for observing a light emission of a sample in a medium with a refractive index n L , the sample being arranged on a surface of a transparent support of refractive index n s , which is greater than n L , the light emission comprising luminous components of a given amplitude and phase, oriented toward the support and forming an angle θ with a direction perpendicular to the surface, said luminous components including supercritical luminous components, for which the angle θ is strictly greater than a critical angle θ c =arcsin(n L /n s ), and also including critical or subcritical luminous components, for which the angle θ is less than or equal to the critical angle θ c , the method comprising: capturing, using an observation device, at least part of the light emission from a region of interest of the sample and obtaining a captured luminous signal comprising luminous components arising from the supercritical luminous components of the light emission; applying filters, using the observation device, to the captured luminous signal in order to selectively decrease the amplitude and/or change the phase of certain luminous components of the captured luminous signal to obtain a filtered luminous signal; and transforming, using the observation device, the filtered luminous signal into an image zone of the region of interest of the sample; wherein: