Patent ID: 9670272
Date: 2017-06-06
CPC Classifications: A61K,A61P,C07K,G01N

Claim:
1. A method of obtaining a monoclonal antibody specific for a pathological variant of an endogenous protein associated with a disorder characterized by the presence of pathological protein structures of the pathological variant of the endogenous protein, wherein the variant of the endogenous protein is derived from beta-amyloid or AIAPP (islet amyloid polypeptide), comprising: (a) subjecting a sample which comprises or is derived from memory B cells from (i) a subject who is symptom-free but affected with, or at risk of developing, the disorder, or (ii) a patient with an unusually stable disease course of the disorder, to a specimen displaying said pathological protein structures of the protein variant and wherein binding of an antibody in the sample to said specimen but not to tissues without said pathological structures indicates the presence in the sample of an antibody specific for the disorder-associated pathological variant of the endogenous protein; and (b) identifying and isolating the antibody which binds to said specimen but not to tissues without said pathological structures as follows: