Patent ID: 9695404
Date: 2017-07-04
CPC Classifications: C12N,C12P,C12Y

Claim:
1. A genetically modified microorganism, comprising a first nucleic acid encoding an Umbellularia californica lauroyl acyl carrier protein (ACP) thioesterase (BTE) operably linked to a promoter, a second nucleic acid encoding a Cocos nucifera lauroyl ACP-thioesterase (FatB3) operably linked to a promoter, and one or more exogenous nucleic acids each operably linked to a promoter, each exogenous nucleic acid encoding a protein selected from the group consisting of an acetyl-CoA carboxylase (ACC), an acetyl-CoA carboxylase carboxyl transferase subunit a (AccA), an acetyl-CoA carboxylase biotin carboxyl carrier protein (AccB), an acetyl-CoA biotin carboxylase (AccC), an acetyl-CoA carboxylase transferase subunit β (AccD), a fatty acid synthase (FAS) subunit, a cytochrome P450 reductase (CPR), a long-chain alcohol oxidase (FAQ1), a long-chain alcohol dehydrogenase (FADH), and an adenosine monophosphate-forming acetyl-coenzyme A synthetase (AceCS), wherein the microorganism is Escherichia coli and produces an increased amount of long-chain dicarboxylic acids as compared to the unmodified parent of the microorganism, wherein the genetically modified microorganism further comprising a loss-of-function mutation in or expressing a lower level of one or more genes selected from the group consisting of a palmitoyl-acyl carrier protein (ACP) thioesterase gene, an acyl-coenzyme A oxidase gene, a citric synthetase (gltA) gene, or an acyl-coenzyme A synthetase (acs) gene.