Patent ID: 9637732
Date: 2017-05-02
CPC Classifications: C12N

Claim:
1. A method of producing mammalian iPS cells, comprising introducing into a mammalian somatic cell (a) expression vectors(s) comprising nucleic acids that encode nuclear reprogramming substances and (b) an expression vector comprising a nucleic acid that encodes a cyclin D family member, and culturing the mammalian somatic cell to form a colony of reprogrammed cells exhibiting characteristics of pluripotency, wherein the nuclear reprogramming substances comprise: (i) Oct3/4, (ii) a member of the Sox family selected from the group consisting of Sox1, Sox2, Sox3, Sox15, and Sox17, and (iii) a member of the Klf family selected from the group consisting of Klf1, Klf2, Klf4 and Klf5.