Patent ID: 9551009
Date: 2017-01-24
CPC Classifications: C07K,C12N,C12P

Claim:
1. A transcription unit comprising a polynucleotide comprising the following regulatory elements: a. the hCMVie virus enhancer, said enhancer having the nucleotide sequence SEQ ID NO: 1, or a nucleic acid having at least 70% sequence identity with the sequence SEQ ID NO: 1 and having transcription activation activity, b. the promoter region of Cyclin-Dependent Kinase 9 (CDK9), said promoter region haying the nucleotide sequence SEQ ID NO: 2, or a nucleic acid having at least 70% sequence identity with the sequence of SEQ ID NO: 2 and having a promoter activity, c. a nucleotide sequence situated downstream of said promoter region and upstream of the translation initiation site, said nucleotide sequence comprising the 5′ untranslated region (5′ UTR) of the eukaryotic Initiation Factor 4G1 (elF4GI) gene, said 5′ UTR having the nucleotide sequence SEQ ID NO: 5, or a nucleic acid having at least 70% sequence identity with the sequence of SEQ ID NO: 5 and having mRNA stabilization and translation facilitator activitites, and d. the intron of the Elongation Factor 1 said transcription unit having the nucleotide sequence SEQ ID NO: 33, or a nucleotide add haying at least 70% sequence identity with the sequence SEQ ID NO: 33, and having said transcription activation activity, said promoter activity, and said mRNA stabilization and translation facilitator activity, wherein said transcription unit is capable of generating a volume production of a protein of interest greater than that obtained with a combination of said CMVie virus enhancer combined with said promoter region of CDK9 alone.