Patent ID: 9637742
Date: 2017-05-02
CPC Classifications: A61K,A61P,C12N

Claim:
1. A method for inhibiting expression of a target gene in a mammalian cell, the method comprising introducing an RNAi-inducing nucleic acid molecule into the cell, wherein the RNAi-inducing nucleic acid molecule comprises: a first strand of 24-119 nt length comprising a region 100% complementary to a target nucleic acid, wherein the region 100% complementary to the target nucleic acid comprises the 19 most 5′ nucleic acids of the first strand; and a second strand of 16 nt length that binds complementarily to the region of the first strand 100% complementary to the target nucleic acid, wherein the second strand binds to the first strand such that the first strand has a double-stranded region to which the second strand binds and a single-stranded region to which the second strand does not bind, and wherein the 5′ end of the first strand and the 3′ end of the second strand form a blunt end.