Patent ID: 9637788
Date: 2017-05-02
CPC Classifications: C12Q

Claim:
1. A method for determining the presence or absence of, or for discriminating between, blood type alleles, which method comprises: providing a DNA-containing sample obtained from a human subject; amplifying a first segment of intron 7 of the RHD locus by allele-specific polymerase chain reaction (PCR) using a first primer comprising the nucleotide sequence of SEQ ID NO: 9 and a second primer comprising the nucleotide sequence of SEQ ID NO: 10, thereby genotyping the sample at at least the TIC polymorphic site at IVS7+1887 and at the C/A polymorphic site at IVS7+2276; amplifying a second segment of intron 7 of the RHD locus by allele-specific PCR using a first primer comprising the nucleotide sequence of SEQ ID NO: 12 and a second primer comprising the nucleotide sequence of SEQ ID NO: 13; probing said amplified second segment by allele-specific hybridization using an oligonucleotide probe having the nucleotide sequence of SEQ ID NO: 70, thereby genotyping the sample at at least the C/T polymorphic site at IVS7+3349; and predicting the blood type allele of the human subject using the genotype of the sample at said polymorphic positions at IVS7+1887, IVS7+2276 and IVS7+3349.