Patent ID: 9593387
Date: 2017-03-14
CPC Classifications: C12Q

Claim:
1. A method for sequencing, detecting, or typing HPV in human samples, comprising the following steps: 1) for each sample, amplifying DNA in the sample with one index primer group, wherein said index primer group comprises 11 index primers, the sequence of each of said index primers comprises an index sequence and a PCR primer sequence, and the index sequence is linked to 5′ end of the PCR primer sequence, optionally via a linker sequence, wherein wherein the index primer groups used in different samples are different, and different index primer groups employ different index sequences; 2) mixing the amplification products obtained by conducting amplification with different index primer groups in step 1), so as to obtain one or more PCR product libraries; 3) adding adapters to the one or more PCR product libraries obtained in step 2) by PCR-free methods, thereby constructing one or more sequencing libraries, wherein the adapters used in different sequencing libraries may be the same or different, and different adapters share the same sequencing sequence but have different characteristic sequences, 4) optionally, mixing the sequencing libraries with different adapters obtained in step 3), so as to obtain one or more library mixtures; 5) conducting sequencing of one or more sequencing libraries obtained in step 3) or one or more library mixtures obtained in step 4) by using a high throughput sequencing technology; 6) correlating the sequencing results to the samples, according to the index primer sequences of the index primer group or according to the index primer sequences of the index primer group and the characteristic sequence of the adapter.