Patent ID: 9580757
Date: 2017-02-28
CPC Classifications: C12Q

Claim:
1. A method for determining the presence or absence of one or more specific serogroups of pathogenic Shiga toxin-producing E. coli (STEC) in a food sample, comprising incubating the food sample in a culture medium enabling growth of performing a lysis of the subjecting the sample solution to a first series of polymerase chain reactions (PCR), using primers to amplify the following and, in case that stx1 and/or stx2 as well as the eae gene and the specific biomarker gene for at least one serogroup are amplified in the first series of PCRs: verifying the presence of the one or more specific serogroups of STEC by performing a second series of PCRs on concentrates of the