Patent ID: 9638692
Date: 2017-05-02
CPC Classifications: G01N,Y02A

Claim:
1. A method for detecting at least two target antibodies in a biological sample comprising: (a) contacting said biological sample with at least one first solid support bound to a 6-alkylguanine-DNA-alkyltransferase (AGT) substrate covalently coupled to a first fusion protein comprising a 6-alkylguanine-DNA-alkyltransferase (AGT) polypeptide comprising SEQ ID NO:2 having a O6-alkylguanine-DNA alkyltransferase activity and a first epitope that is recognized by a first target antibody; (b) contacting said biological sample with at least one second solid support bound to a 6-alkylguanine-DNA-alkyltransferase (AGT) substrate covalently coupled to a second fusion protein comprising a 6-alkylguanine-DNA-alkyltransferase (AGT) polypeptide having a O6-alkylguanine-DNA alkyltransferase activity and a second epitope that is recognized by a second target antibody, but not by said first target antibody; and (c) detecting the presence or absence of the two target antibodies by detecting the binding or lack of binding of the two target antibodies to the two fusion proteins.