Patent ID: 9701717
Date: 2017-07-11
CPC Classifications: A61K,A61P,C07K

Claim:
1. An isolated Alphabody polypeptide capable of specifically binding to interleukin-23 (IL-23) comprising an amino acid sequence having the general formula HRS1-L1-HRS2-L2-HRS3, wherein each of HRS1, HRS2 and HRS3 is independently a heptad repeat sequence (HRS) comprising 2 to 7 consecutive but not necessarily identical heptad repeat units, wherein said heptad repeat units are 7-residue (poly)peptide fragments represented as ‘abcdefg’ or ‘defgabc’, wherein the symbols ‘a’ to ‘g’ denote conventional heptad positions and wherein said heptad repeat sequence is represented by (abcdefg)n or (defgabc)n wherein n is between 2 and 7, wherein at least 50% of all heptad a- and d-positions are occupied by isoleucine residues, wherein each HRS starts with an aliphatic or aromatic amino acid residue located at a heptad a-position or ‘d” position, wherein each of L1 and L2 are independently a linker fragment, which covalently connect HRS1 to HRS2 and HRS2 to HRS3, respectively, characterized in that in HRS1: (i) the heptad f-position of the second heptad repeat unit is occupied by glutamic acid or alanine, (ii) the heptad g-position of the second heptad repeat unit is occupied by valine, (iii) the heptad c-position of the third heptad repeat unit is occupied by alanine or glycine, and (iv) the heptad g-position of the third heptad repeat unit is occupied by tyrosine or tryptophan, and in HRS3: (i) the heptad e-position of the second heptad repeat unit is occupied by glutamine or lysine, (ii) the heptad b-position of the third heptad repeat unit is occupied by leucine or isoleucine or valine, (iii) the heptad e-position of the third heptad repeat unit is occupied by tyrosine, and (iv) HRS3 comprises a partial (a-f) heptad repeat unit following the third heptad repeat and the heptad b-position therein is occupied by methionine.