Patent ID: 9745616
Date: 2017-08-29
CPC Classifications: C12Q

Claim:
1. A method for detecting a plurality of different target nucleic acids in a sample, wherein each target nucleic acid comprises a first target domain adjacent to a second target domain and a third target capture domain located upstream or downstream from said first and second target domains, said method comprising: (a) providing a plurality of ligation substrates each comprising: (b) ligating said first and second ligation probes without the use of a ligase enzyme to form a first plurality of ligation products; (c) hybridizing target capture probes comprising a capture moiety to said third target domain of said target nucleic acids to form target complexes; (d) capturing said target complexes on a surface using said capture moiety; (e) amplifying said ligation products to form amplicons; (f) detecting said amplicons, thereby detecting said target nucleic acids.