Patent ID: 9719141
Date: 2017-08-01
CPC Classifications: A61P,C07K,C12Q,G01N

Claim:
1. A method for detecting an over-expressed immunoglobulin nucleic acid sequence from a clonally expanded B cell population in a subject comprising (a) providing a cDNA sample from the subject, wherein the subject is infected with hepatitis C virus (HCV) but is asymptomatic for cryoglobulinemic vasculitis and B cell malignancy; (b) contacting the sample with a set of first primers and second primers wherein said set of first primers and second primers comprises a first primer and a second primer that flank the complementary determining region 3 (CDR3) sequence, a first primer and a second primer that flank a VH-D-JH sequence and a first primer and a second primer that flank a VK-JK sequence in Ig-encoding transcripts; (c) amplifying said CDR3 sequence, said VH-D-JH sequence, and said VK-JK sequence; and (d) detecting an overexpressed nucleic acid sequence encoding an amino acid sequence comprising IgH V1-69 or IgHV V 3-7, VK325 or VK 328, JH4 or JH3, Jk1, and a D region consensus sequence comprising SEQ ID NO: 1 or SEQ ID NO: 2.