Patent ID: 9738937
Date: 2017-08-22
CPC Classifications: C12Q,G01N

Claim:
1. A method for identifying candidate target cells within a biological fluid specimen, comprising: obtaining a biological fluid specimen; preparing the biological fluid specimen by staining cell nuclei in the biological fluid specimen with a first bio-conjugated dye having a first color and configured to bind nucleic acids in the cell nuclei of the target cells, and staining cytoskeletal cell features in the biological fluid specimen with a second bio-conjugated dye having a second color and configured to bind to cytoskeletal cell features of the target cells, and staining white blood cells in the biological fluid specimen with a third bio-conjugated dye having a third color and configured to bind to human leukocyte antigens in the biological fluid specimen; capturing a digital image of the biological fluid specimen, the digital image having a plurality of color channels; and applying image analysis to the digital image including