Patent ID: 9587269
Date: 2017-03-07
CPC Classifications: C12Q

Claim:
1. A method for generating single-stranded nucleic acids, comprising: a) hybridizing a first primer to a nucleic acid molecule; b) extending the first primer via polymerization under polymerization conditions, thereby forming an extended first primer product that is complementary to a portion of the nucleic acid molecule; c) introducing a nick into a portion of the nucleic acid molecule that is hybridized to the extended first primer product, where the nick includes a free 5′ end and a free 3′ end in the nucleic acid molecule; and d) degrading the nucleic acid molecule from the free 5′ end of the nick using a degrading agent, thereby generating a single-stranded extended primer product which remains hybridized to an undegraded portion of the nucleic acid molecule.