Patent ID: 9658220
Date: 2017-05-23
CPC Classifications: C12N,C12Y,G01N

Claim:
1. A method for normalizing results, the method comprising: (a) contacting a biological sample with one or more solid supports having binding members immobilized thereon that specifically bind an analyte in the biological sample, under conditions promoting binding of the analyte to the binding members, the solid supports being divided into subpopulations that are differentiable from each other by a differentiation parameter comprising a characteristic that is independent of the binding members immobilized on the solid supports, the binding members immobilized on each subpopulation binding to one analyte in the sample; (b) contacting the biological sample with a solid support having human blood coagulation Factor XIII (hFXIII) immobilized thereon, the solid support being differentiable from the solid supports having binding members immobilized thereon; (c) contacting the solid supports with a binding agent having binding affinity for hFXIII and binding agents having binding affinity for each of the analytes, under conditions promoting binding of the binding agents to the analytes on the solid supports; (d) quantifying the binding agents bound to the solid supports, and correlating the quantity of binding agents with the differentiation parameters to obtain values individually representative of levels of the analytes and of hFXIII; and (e) normalizing the values of the levels representative of the analytes to the value representative of hFXIII, thereby correcting for variations and decreasing the coefficient of variation in sample processing or biological matrix effects during performance of the method, wherein the normalizing comprises dividing the values of the levels representative of the analytes by the value representative of hFXIII.