Patent ID: 9606106
Date: 2017-03-28
CPC Classifications: A61B,A61P,G01N,G01R

Claim:
1. A method for monitoring metabolism of a substrate within a given type of cell in a sample, the method comprising: a. culturing a given type of cell of a first sample with a substrate for a sufficient period of time to allow metabolic breakdown of the substrate into substrate metabolites, wherein at least a portion of the substrate is labeled with a nuclear magnetic resonance (NMR) stable isotope; b. harvesting the substrate metabolites from the cells of step (a) to obtain a second sample of substrate metabolites; and c. performing multi-dimensional NMR on the second sample of step (b) to determine a resonance spectrum of the metabolized substrate, wherein the resonance spectrum represents the metabolites of the substrate, and wherein the multi-dimensional NMR comprises any one of the following techniques: