Patent ID: 9540684
Date: 2017-01-10
CPC Classifications: A61B,A61K,C12Q,G01N

Claim:
1. A method for detecting one or more biomarkers from a tissue or biological sample of a subject, comprising: (a) providing a device comprising one or more microneedles to which one or more probes specific for the one or more biomarkers are covalently attached, wherein the one or more probes comprise polynucleotides complementary to the one or more biomarkers; (b) contacting the microneedle device with the tissue or biological sample of the subject such that the one or more probes complementary to the one or more biomarkers hybridize to the one or more biomarkers in situ, thereby binding the one or more biomarkers to the one or more microneedles; and (c) detecting the one or more biomarkers that are bound to the one or more probes on the one or more microneedle, wherein at least one biomarker is an mRNA polynucleotide and wherein the one or more biomarkers bound to the one or more probes on the one or more microneedles are present in quantities amenable to detection following elution from the one or more microneedles, and wherein the method is able to detect mRNA biomarkers present in the tissue or biological sample at a concentration of 1.66 pM.