Patent ID: 9622459
Date: 2017-04-18
CPC Classifications: A01K,C07K,C12N

Claim:
1. A method for making an antibody that binds to an antigen of interest, wherein the method comprises: (a) exposing a mouse to an antigen of interest, (b) isolating one or more B lymphocytes of the mouse, wherein the one or more B lymphocytes express an antibody that binds the antigen of interest, wherein the expressed antibody that binds the antigen of interest includes an immunoglobulin light chain comprising a human immunoglobulin λ light chain variable domain and a mouse immunoglobulin light chain constant domain, and comprises an immunoglobulin heavy chain comprising a human immunoglobulin heavy chain variable domain and a mouse immunoglobulin heavy chain constant domain, (c) identifying a nucleic acid sequence that encodes the human immunoglobulin λ light chain variable domain of the antibody that binds the antigen of interest, and (d) employing the nucleic acid sequence of (c) with a human immunoglobulin light chain constant region nucleic acid sequence to make a human immunoglobulin light chain for the antibody that binds the antigen of interest; wherein the mouse comprises