Patent ID: 9598672
Date: 2017-03-21
CPC Classifications: A61K,A61P,C12N,Y10T

Claim:
1. A kit comprising a first active fraction of conditioned media and a second active fraction of conditioned media, wherein the first active fraction and the second active fraction are prepared by a method comprising the steps of: (a) culturing cells capable of generating extracellular matrix on a base matrix in a media for culturing said cells; (b) harvesting an unfractionated conditioned media from said cells, wherein the source of said cells is selected from the group consisting of corneal endothelial cells and retinal pigment epithelial (RPE) cells; (c) separating said first active fraction and said second active fraction from said unfractionated conditioned media by a first centrifugal filtration and a second centrifugal filtration, wherein said first centrifugal filtration uses a 50 kDa molecular weight cutoff filter for said first active fraction and said second centrifugal filtration uses a <3 kDa molecular weight cutoff filter for said second active fraction, and wherein said first centrifugal filtration is applied directly to said unfractionated conditioned media and said second centrifugal filtration is applied to either one of (i) directly to said unfractionated conditioned media or (ii) to said first active fraction; and (d) collecting said first active fraction and said second active fraction, wherein the kit does not comprise unfractionated conditioned media.