Patent ID: 9655927
Date: 2017-05-23
CPC Classifications: A61K,A61P,C12N

Claim:
1. A method for inhibiting a peripheral inflammatory response in a subject, the method comprising administering to the subject an effective amount of cells obtained by a method comprising: (a) providing a culture of marrow adherent stromal cells; (b) contacting the cell culture of step (a) with a polynucleotide comprising sequences encoding a Notch intracellular domain (NICD) wherein said polynucleotide does not encode a full-length Notch protein: (c) selecting cells that comprise the polynucleotide of step (b); and (d) further culturing the selected cells of step (c) in the absence of selection for the polynucleotide; wherein inhibition of the peripheral inflammatory response is mediated by one or more of: (i) inhibition of helper T-cell proliferation; (ii) expansion of a regulatory T-cell population; (iii) inhibition of differentiation of a monocyte to a dendritic cell; (iv) inhibition of dendritic cell maturation; (v) alteration of the cytokine profile of a T-cell or a monocyte from one that is pro-inflammatory to one that is anti-inflammatory; and (vi) reduced production of interleukin-10 (IL-10) by a T-cell or a monocyte.