Patent ID: 9540674
Date: 2017-01-10
CPC Classifications: A61P,C12N,C12Q,G01N

Claim:
1. A method comprising: (a) providing a cancer cell sample; (b) permeabilizing a first aliquot of cells from the cancer cell sample; (c) contacting the first aliquot of permeabilized cells with a first purified BH3 domain peptide of 50 amino acids or less; (d) measuring the first BH3 domain peptide-induced mitochondrial outer membrane permeabilization (MOMP) in the first aliquot of cells; (e) permeabilizing a second aliquot of cells from the cancer cell sample; (f) contacting the second aliquot of permeabilized cells with a second purified BH3 domain peptide of 50 amino acids or less, wherein the first BH3 domain peptide and the second BH3 domain peptide are derived from different BCL-2 proteins; (g) measuring the second BH3 domain peptide-induced MOMP in the second aliquot of cells; and (h) determining a test BH3 profile for the cancer cell sample based on the first BH3 domain peptide-induced MOMP measured in (d) and the second BH3 domain peptide-induced MOMP measured in (g).