Patent ID: 9618516
Date: 2017-04-11
CPC Classifications: G01N,H05K

Claim:
1. A method for measuring a binding property between a compound and a target macromolecule, comprising: (a) preparing a fluid mixture comprising (i) a chimeric molecule comprising a target macromolecule subject to denaturation linked to a labeling peptide and (ii) a compound being measured for binding to the target macromolecule; (b) incubating the fluid mixture of step (a) under conditions permitting binding of said compound to target macromolecules in the mixture; (c) treating the fluid mixture of step (b) under conditions that cause a differential denaturation between chimeric molecules bound to the compound and chimeric molecules not bound to the compound; and (d) generating a signal from chimeric molecules subjected to said differential denaturation in step (c), by adding to the mixture a second label that reacts with the labeling peptide in the chimeric molecule, wherein, (e) the signal in step (d) is detected and indicates a binding property between the compound and the target macromolecule.