Patent ID: 9648813
Date: 2017-05-16
CPC Classifications: A01H,C12N,C12Q

Claim:
1. A method for producing a plant cell or plant with at least one of: a) increased GDP-L-Galactose phosphorylase (GGP) translation, b) increased GGP production, c) increased GGP activity, and d) increased ascorbate production, the method comprising modification of the 5′-UTR of a GGP gene in the plant cell or plant, wherein the 5′UTR comprises at least one of: i) a sequence with at least 70% identity to any one of SEQ ID NOs: 41-100, and 111-131, and ii) a sequence encoding a polypeptide with at least 70% identity to any one of SEQ ID NOs: 1-40, 108, and 132 to 140; wherein the modification disrupts the function of an upstream open reading frame (uORF) encoded by the 5′UTR; and wherein said modification is at least one of a deletion, an addition, or a substitution of at least one nucleotide in the 5′UTR.