Patent ID: 9611499
Date: 2017-04-04
CPC Classifications: A61K,C07K,C12P

Claim:
1. A method for producing a recombinant Type 1 interferon protein, said method comprising: expressing the recombinant Type I interferon protein by culturing a lysing the cultured host cell; obtaining an insoluble fraction and a soluble fraction from the lysed host cell; extracting the insoluble fraction by subjecting it to non-denaturing extraction conditions; wherein the non-denaturing extraction conditions comprise a Zwitterionic detergent at a concentration of about 0.5% to about 2%, to obtain soluble recombinant Type 1 interferon protein without the use of a renaturing or refolding step; separating the obtained soluble recombinant Type 1 interferon protein from other protein and cellular debris; and measuring the activity of the separated soluble recombinant Type 1 interferon protein, wherein about 80% to about 100% of the separated soluble recombinant Type 1 interferon protein is determined to be active.