Patent ID: 9598717
Date: 2017-03-21
CPC Classifications: C07K,C12M,C12P,C12Q

Claim:
1. An enzyme treatment method using a hollow fiber membrane, the enzyme treatment method comprising: injecting a protein and an enzyme into a hollow fiber membrane, said enzyme being capable of digesting the protein, wherein the hollow fiber membrane has a first end and a second end opposite to the first end and receives the protein and the enzyme through the first end, wherein the second end of the hollow fiber membrane is closed, and wherein the hollow fiber membrane has a molecular weight cutoff value of 10 kDa; digesting the protein using the enzyme inside the hollow fiber membrane to produce peptides; collecting the peptides from the inside the hollow fiber membrane, said peptides having a molecular weight smaller than the molecular weight cutoff value of the hollow fiber membrane and passing across the hollow fiber membrane; and eluting and discharging the collected peptides, wherein the protein is a glycoprotein, wherein the enzyme is peptide N-glycosidase F, and wherein the hollow fiber membrane is housed by a tubing, said tubing surrounding the hollow fiber membrane and collecting the peptides which pass across the hollow fiber membrane.