Patent ID: 9562082
Date: 2017-02-07
CPC Classifications: C07K,C12N,C12P

Claim:
1. A glycoprotein produced by a method comprising the steps of: (a) cultivating eukaryotic cells in a medium comprising the additive DMSO, N- acetylmannosamine (NAcMan), or N-acetylglucosamine (NAcGlc), or any combination of two or more of such additives, wherein the medium additionally comprises iron in a concentration range from 0.05 to 0.1 mM, and (b) recovering from that medium, or from said cells, the glycoprotein, wherein the glycoprotein exhibits (i) a degree of sialylation increased by at least 5% , when compared with the degree of sialylation of the same glycoprotein when produced in the same medium but without the additive or additives; and (ii) a content of Neu5Gc (N- glycolylneuraminic acid) that is at least 50% lower than the content of Neu5Gc of the same glycoprotein when produced in the same medium but without the additive or additives.