Patent ID: 9555105
Date: 2017-01-31
CPC Classifications: A61K,A61P,C12N,G01N

Claim:
1. An isolated artificial antigen presenting cell (aAPC) comprising a K562 cell transduced with a lentiviral vector (LV), wherein said LV comprises a nucleic acid encoding at least one co-stimulatory ligand that specifically binds with at least one co-stimulatory molecule selected from the group consisting of CD27, CD28, 4-1BB, OX40, CD30, CD40, PD-1, ICOS, LFA-1, CD2, CD7, LIGHT, NKG2C, B7-H3, BTLA, Toll ligand receptor and a ligand that specifically binds with CD83, and said LV further comprises a nucleic acid encoding a CD64 molecule, and wherein said aAPC expresses said co-stimulatory ligand and said CD64 molecule loaded with anti-CD3 antibody, and further wherein said aAPC can stimulate and expand a T cell contacted with said aAPC.