Patent ID: 9651567
Date: 2017-05-16
CPC Classifications: B01L,C12Q,G01N

Claim:
1. An in vitro method for measuring an activity of a coagulation factor of the intrinsic or extrinsic pathway, wherein said coagulation factor comprises any one of Factor II, Factor V, Factor VII, Factor VIII, Factor IX, Factor X, Factor XI, and Factor XII, said method comprising: (i) contacting a capillary blood sample comprising whole blood from a test subject with plasma depleted of said coagulation factor, an ionic citrate source, an ionic calcium source, phospholipids, and one or more of a coagulation contact phase activator and tissue factor; (ii) determining the time required for blood coagulation of said capillary blood sample; and (iii) correlating said time with said activity of said coagulation factor, wherein step (i) takes place on a surface of a test strip that comprises a reaction area that comprises a plurality of communicating compartments, wherein the first compartment of said plurality of compartments contains said plasma depleted of said coagulation factor and said ionic citrate source, and wherein the second compartment of said plurality of compartments contains one or more of said coagulation contact phase activator and said tissue factor.