Patent ID: 9597386
Date: 2017-03-21
CPC Classifications: A61K,A61P,C07K,G01N

Claim:
1. An immunogenic composition comprising at least one outer membrane protein (OMP) from Histophilus somni selected from the group consisting of PTA 12755, PTA 12756 and combinations thereof and a physiologically-acceptable vehicle, wherein the OMP is generated by incubating Histophilus somni bacteria, suspending Histophilus somni bacteria in 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) buffer containing protease inhibitors, lysing the Histophilus somni bacteria by cycles of freezing and thawing, and further Histophilus somni bacteria lysing by sonication cycles, resuspending in HEPES containing protease inhibitors, and then solubilizing non-protein components and then extracting solely by SDS extraction.