Patent ID: 9678087
Date: 2017-06-13
CPC Classifications: G01N,Y10T

Claim:
1. A method for determining the amount of estrone in a human plasma or serum sample, said method comprising: (a) adding deuterated estrone to a human plasma or serum sample; (b) processing the sample by high performance liquid chromatography (HPLC); (c) ionizing estrone and deuterated estrone in said processed sample with electrospray ionization (ESI) in positive ion mode to produce one or more ions detectable by mass spectrometry; and (d) detecting the amount of the ions by tandem mass spectrometry; (e) comparing the amount of said one or more ions of estrone and said one or more ions of deuterated estrone to determine the amount of estrone in the sample; wherein said method has a limit of quantitation less than or equal to 500 pg/mL.