Patent ID: 9617523
Date: 2017-04-11
CPC Classifications: A61P,C12N,C12Y

Claim:
1. A recombinant mammalian cell comprising an exogenous recombinant nucleic acid encoding human vitamin K reductase complex subunit 1 (VKORC1) inserted into a plasmid and an exogenous recombinant nucleic add coding for a vitamin K dependent (VKD) procoagulant blood factor protein, wherein both the VKORC1 and the VKD protein are expressed in the mammalian cell, and wherein the mammalian cell does not overexpress γ-carboxylase; wherein the mammalian cell is selected from the group consisting of CHO cells, NSO cells, sp20 cells, Perc6 cells, SkHep cells, BHK cells, Hela cells, Vero cells, COS cells, and HEK293 cells, and wherein the procoagulant blood factor selected from the group consisting of Factor II, Factor VII, Factor IX and Factor X.