Patent ID: 9557321
Date: 2017-01-31
CPC Classifications: C12N,G01N

Claim:
1. A method for obtaining a drug for suppressing inflammasome induction of IL-1β production and secretion, comprising the steps of: (a) contacting a test substance with LPS-stimulated macrophages derived from induced pluripotent stem (iPS) cells having mutant NLRP3 genes; (b) measuring the amount of IL-1β secretion from the macrophages after step (a); (c) selecting as a drug for suppressing inflammasome induction of IL-1β production and secretion the test substance which makes the amount of secretion measured in step (b) equivalent to or less than the amount of IL-1β secretion from LPS-stimulated macrophages derived from iPS cells having wild-type NLRP3 genes; and (d) obtaining the drug for suppressing inflammasome induction of IL-1β production and secretion based upon the selection in step (c), wherein said mutant NLRP3 genes have a nucleotide substitution which replaces adenine at position 1709 with guanine counted from the translation initiation codon, a nucleotide substitution which replaces cytosine at position 1043 with thymine counted from the translation initiation codon, or a nucleotide substitution which replaces guanine at position 587 with adenine counted from the translation initiation codon, wherein said iPS cells having mutant NLRP3 genes and said iPS cells having wild-type NLRP3 genes are iPS cells derived from the same individual.