Patent ID: 9556460
Date: 2017-01-31
CPC Classifications: C12N,C12P,C12Y,Y02P

Claim:
1. A method of producing 3 hydroxy-3-methylbutyric acid comprising: (a) providing a recombinant microorganism, wherein the recombinant microorganism is genetically modified to overexpress a polynucleotide encoding a 3-hydroxy-3-methylglutaryl-CoA (HMG-CoA) synthase (EC 2.3.3.10) as compared to the same recombinant microorganism without the genetic modification, and wherein the recombinant microorganism is genetically modified to overexpress a polynucleotide encoding an acetoacetate decarboxylase as compared to the same recombinant microorganism without the genetic modification; and (b) incubating, in the recombinant microorganism (i) acetone, (ii) acetyl-coenzyme A, and (iii) the HMG-CoA synthase, to thereby produce 3-hydroxy-3-methylbutyric acid.