Patent ID: 9650412
Date: 2017-05-16
CPC Classifications: B01D,C07K,C12M,C12N,C12P,C12Y

Claim:
1. A biological manufacturing system for obtaining a recombinant therapeutic protein in a fluid, comprising: a first periodic counter current chromatography system (PCCCS1) comprising an inlet; and a second periodic counter current chromatography system (PCCCS2) comprising an outlet, wherein the PCCCS1 and the PCCCS2 are in fluid communication with each other; the biological manufacturing system is configured such that the fluid is passed into the inlet, through the PCCCS1 and the PCCCS2, and exits the biological manufacturing system through the outlet; the PCCCS1 is configured to perform at least the unit operation of capturing the recombinant therapeutic protein by a resin; and the PCCCS2 is configured to perform at least two unit operations of purifying and polishing the recombinant therapeutic protein by resins, wherein the resin used in purifying is different in chemical structure compared to the resin used in the polishing.