Patent ID: 9714413
Date: 2017-07-25
CPC Classifications: A61K,A61P,C12N

Claim:
1. A method of producing non-exhausted immature dendritic cells (DCs), comprising the steps of: providing a mixture of allogeneic leukocytes, which allogeneic leukocytes have been obtained from at least two different, allogeneic donors; isolating allogeneic monocytes from said mixture of allogeneic leukocytes to provide monocyte-enriched allogeneic leukocytes; and generating non-exhausted immature DCs from said monocyte-enriched allogeneic leukocytes, wherein the generation of non-exhausted immature dendritic cells (DCs) is performed by co-culturing said monocyte-enriched allogeneic leukocytes for 2 to 7 days in aqueous cell culture medium free from non-human serum, said medium being supplemented with interleukin-4 (IL-4) and granulocyte-macrophage colony stimulating factor (GM-CSF).