Patent ID: 9534253
Date: 2017-01-03
CPC Classifications: B82Y,C12Q,G01N,Y10S

Claim:
1. A system for sequencing a double strand molecule, the system comprising: a membrane comprising a first Y-channel, the first Y-channel comprises a first stem and a first left branch and a first right branch, the first branches and the first right branch being connected to the first stem at a first junction; a second Y-channel comprising a second stem, a second left branch, and a second right branch formed in the membrane, the second left and right branches being connected to the second stem at a second junction, wherein the second stem of the second Y-channel is connected to the first left branch of the first Y-channel such that the first left branch feeds into the second Y-channel wherein the second junction comprises a sharp end configured to separate base pairs, wherein the first stem comprises a diameter of 4-10 nm, the first left branch comprises a diameter of 2-4 nm, the first right branch comprises a diameter of 2-4 nm, and the sharp end of the second junction comprises an angle of 30-120 degrees; a top fluidic chamber on one side of the membrane and a bottom fluidic chamber on an opposing side of the membrane; a first voltage pulse of a voltage source drives the double strand molecule into the first Y-channel of the membrane; wherein the double strand molecule is slowed at the junction of the first Y-channel based on the first voltage pulse being weaker than a force required to break a base pair of the double strand molecule; a second voltage pulse of the voltage source drives the double strand molecule into the junction of the first Y-channel to split the double strand molecule into a first single strand and a second single strand; wherein the first single strand is sequenced in one of the branches and the second single strand is sequenced in another one of the branches.