Patent ID: 9611505
Date: 2017-04-04
CPC Classifications: C12Q

Claim:
1. A method of reverse transcription, wherein the method comprises the steps of a) providing a sample comprising RNA, b) contacting the sample with a carrier nucleic acid thereby generating a mixture, wherein the carrier nucleic acid is polyA-RNA, c) applying the mixture to a matrix under conditions such that binding of the RNA and the carrier nucleic acid to the matrix occurs, d) separating the matrix with the RNA and the carrier nucleic acid bound to the matrix from the mixture, e) eluting the RNA and the carrier nucleic acid from the matrix thereby generating an eluate, f) adding a blocking nucleic acid to the eluate under conditions such that hybridization of the blocking nucleic acid to the carrier nucleic acid occurs, wherein the blocking nucleic acid is an oligo-dT molecule modified at the 3′ end to prevent extension of the oligo-dT molecule by a polymerase reaction, g) adding a primer to the eluate from step f) under conditions such that hybridization of the primer to the RNA occurs, and h) reverse transcribing the RNA into cDNA.