Patent ID: 9702012
Date: 2017-07-11
CPC Classifications: A61P,C12N,C12Q

Claim:
1. A decoy polynucleotide comprising a sequence comprising a binding site for a cis-regulatory factor, wherein the binding site is not operably linked to a gene, and wherein the cis-regulatory factor is a regulator of expression of one or more antibiotic resistance genes in a prokaryote, wherein the decoy polynucleotide causes reduction in the expression of the one or more antibiotic resistance genes and reduces antibiotic resistance of the prokaryotic cell; and wherein the decoy polynucleotide is a linear oligonucleotide with at least one element of secondary structure to increase nuclease resistance of the polynucleotide, wherein the at least one element of secondary structure is a stem-loop structure and wherein sequence comprising the transcription factor binding site is adjacent to heterologous sequence.