Patent ID: 9580500
Date: 2017-02-28
CPC Classifications: A61K,A61P,C07K,C12N,G01N

Claim:
1. A method of producing an antibody capable of binding a proTGF-β growth factor prodomain complex (GPC) comprising: a. forming a protein complex of pro-transforming growth factor beta 1 (proTGF-β1) GPC and glycoprotein-A repetitions predominant protein (GARP); b. utilizing the protein complex formed in (a) as a target antigen in solid-phase or solution-phase enrichment with an antibody fragment phage display library; c. selecting phage particles that bind to the protein complex formed in (a) and which express antibody fragments on their surface, and d. producing recombinant antibodies that bind the protein complex formed in (a) and that have complementarity determining regions (CDRs) obtained from the antibody fragments expressed at the surface of the selected phage particles of (c).