Patent ID: 9567565
Date: 2017-02-14
CPC Classifications: A61K,C07K,C12M,C12N

Claim:
1. A static culture method of expanding a population of cells with a gas permeable cell culture device having a growth surface comprised of liquid impermeable gas permeable material, at least a portion of said gas permeable material in contact with ambient gas, the static culture method comprising: adding cells, which include responder and irradiated feeder cells, and medium into the gas permeable cell culture device, the ratio of the volume of said medium to the surface area of said growth surface being 3 ml/cm allowing static cell culture for a period of time and not placing additional medium into the gas permeable cell culture device during said period of time, and said period of time is at least 3 days, and adding at least one type of cytokine after the onset of static cell culture.