Patent ID: 9611497
Date: 2017-04-04
CPC Classifications: C12N,C12P,C12Q

Claim:
1. A method for measuring a level of RNA in a sample of cells using real time PCR, the method comprising: preparing an admixture of the sample, a proteinase K and a deoxyribonuclease, wherein the concentration of cells in the admixture is up to 2500 cells per μl; incubating the admixture under conditions where the proteinase K and the deoxyribonuclease are active to produce a cell lysate; incubating the cell lysate at a temperature up to about 75° C. wherein the proteinase K and the deoxyribonuclease are inactivated; incubating a volume of cell lysate with at least one reverse transcriptase under conditions to allow reverse transcription to produce cDNA, amplifying the cDNA using real-time PCR to produce amplified cDNA, measuring the amount of amplified cDNA, and calculating the level of RNA based on the measured amount of amplified cDNA.