Patent ID: 9556469
Date: 2017-01-31
CPC Classifications: C12Q

Claim:
1. A method for determining the presence or absence of at least one microorganism in a biological medium, said method comprising at least: providing an enclosure containing: exposing at least said liquid or semi-solid phase to conditions that are favorable for said microorganism to metabolize said enzymatic substrate into at least one molecule of said VOC volatile metabolite; and determining, by optical transduction, the presence or absence of said VOC metabolite, an indicator of the presence of said microorganism; wherein: said VOC metabolite, if formed, interacts with a nanoporous matrix which has an affinity for said VOC volatile metabolite, said matrix being in a form that is separate from said enzymatic substrate and arranged in the gas phase; the detection by optical transduction of a change in the optical properties of said matrix indicates that said matrix interacts with said metabolite; and VOC is a metabolite specific to the metabolization of the enzymatic substrate and representative of an enzymatic route specific to the microorganism subject of detection.