Patent ID: 9607374
Date: 2017-03-28
CPC Classifications: G06K,G06T

Claim:
1. A method for blind color decomposition of histological tissue image data, image data comprising a set of pixels, into density maps where each density map corresponds to the portion of the original image data that contains one stain/tissue combination, the method comprising the steps of: capturing of histological tissue image data corresponding to a tissue sample that has been stained with at least one stain, the said stain or stains being light absorbent; transforming the histological tissue image data to optical density data, the transformation utilizing the Beer-Lambert law of absorption; projecting the optical density data to the Maxwellian Chromaticity plane resulting in a pure color data representation that is decoupled from the intensity in the transformed image data; and identifying at least one reference color in the pure color data representation in the Maxwellian Chromaticity plane, using statistical techniques.