Patent ID: 9738938
Date: 2017-08-22
CPC Classifications: C12Q

Claim:
1. A method of determining the presence of community-associated methicillin-resistant Staphylococcus aureus (CA-MRSA) in a human, comprising the steps of: (a) obtaining a biological sample from a human suspected of having a CA-MRSA infection, wherein said biological sample is fluid or tissue; (b) isolating genomic material from said biological sample; (c) performing a polymerase chain reaction (PCR) targeted against the penicillin binding protein 3 gene (SEQ ID NO: 39) present in said isolated genomic material to generate an amplicon containing the SNP G88A by using a forward primer consisting of SEQ ID NO: 1 and a reverse primer consisting of SEQ ID NO: 2; (d) performing a sequencing reaction on said generated amplicon by using a sequencing primer consisting of SEQ ID NO:1; (e) detecting the presence of adenine (A) at nucleotide position 88 (SNP G88A) in said penicillin binding protein 3 gene in said amplicon; and (f) determining the presence of CA-MRSA in said human by said presence of said adenine at said nucleotide position 88 (SNP G88A) in said amplicon.