Patent ID: 9657355
Date: 2017-05-23
CPC Classifications: C12Q

Claim:
1. A method for detecting the presence or absence of any species of a fungus belonging to the genus Pneumocystis in a sample, comprising i. obtaining a sample suspected of containing fungal nucleic acid from the genus ii. contacting said sample with a forward primer consisting of SEQ ID NO: 19 and a reverse primer consisting of SEQ ID NO: 20 in the presence of one or more internal PCR amplification controls under conditions under which PCR may occur; and iii. detecting the presence or absence of an amplified identifying region of fungal nucleic acid in the sample, wherein said identifying region of fungal nucleic acid comprises SEQ ID NO: 18, the complement or transcript thereof, or a sequence having 80% or more sequence identity with SEQ ID NO: 18, the complement or transcript thereof, said identifying region being present in species of the genus wherein the presence of said amplified identifying region of fungal nucleic acid in the sample indicates the presence of any species of a fungus belonging to the genus