Patent ID: 9587265
Date: 2017-03-07
CPC Classifications: C12N,C12Q,G01N

Claim:
1. A method for determining a presence or absence of a linkage type in a polyubiquitin chain, comprising the steps of: (a) contacting an ubiquitin polymer of unknown linkage type with a first linkage-specific deubiquitinase under conditions in which the enzyme catalyses the disassociation of one or more, but less than seven, ubiquitin linkages; (b) analysing the product of the catalysis for the presence of lower molecular weight fractions of the ubiquitin polymer; (c) repeating steps (a) and (b) in the presence of a second, different linkage-specific deubiquitinase; and (d) identifying the presence or absence of a linkage type cleaved by the first or second deubiquitinase by the presence or absence of lower molecular weight fractions of the ubiquitin polymer in the analysis of step (b).