Patent ID: 9637540
Date: 2017-05-02
CPC Classifications: A61K,C07K,C12N,G01N

Claim:
1. A method for producing an immunoglobulin in an isolated B lymphocyte cell line comprising: isolating from a vertebrate subject exposed to at least one first antigen, a B lymphocyte cell, and growing the B lymphocyte in vitro to obtain a B lymphocyte cell line expressing at least one endogenous secreted immunoglobulin reactive to the at least one first antigen; introducing into the isolated B lymphocyte cell line at least one exogenous membrane immunoglobulin reactive to at least one second antigen to produce a recombinant B lymphocyte cell line; selecting the isolated B lymphocyte cell line expressing the membrane immunoglobulin reactive to the at least one second antigen and expressing the at least one endogenous secreted immunoglobulin reactive to the at least one first antigen.