Patent ID: 9726605
Date: 2017-08-08
CPC Classifications: G01N

Claim:
1. A fluorescence immunochromatography method carried out by illuminating a fluorescent substance in a membrane with excitation light and detecting fluorescence emitted from the fluorescent substance, the fluorescence immunochromatography method comprising the steps of: applying a sample to a test strip having a membrane and a transparent film attached to the surface of the membrane, (n (n binding the fluorescent labeling reagent to the target substance; immobilizing the fluorescent labeling reagent bound to the target substance in the membrane; detecting the presence of target substance by illuminating the fluorescent labeling reagent bound to the target substance in the membrane with excitation light through the transparent film, thereby bringing fluorescence components into a guided wave within the transparent film, detecting another fluorescence components not exceeding the critical angle, wherein the another fluorescence components are included in the fluorescence emitted from the fluorescent substance, the detection being made by virtue of the fluorescence components passing through the film.