Patent ID: 9648857
Date: 2017-05-16
CPC Classifications: A01K,A61K

Claim:
1. A method for testing the immunogenicity of a variant antigen in a transgenic mouse model of tolerance in the human immune system, where the variant antigen is derived from a mammalian antigen, and is tested in a transgenic mouse that is transgenic for both human MHC class II molecules and the mammalian antigen, the method comprising: (a) obtaining said transgenic mouse that is transgenic for both human MHC class II molecules and the mammalian antigen by backcrossing a human HLA transgenic mouse with mice that are transgenic for the mammalian antigen, wherein the transgenic mouse has tolerance for the mammalian antigen, wherein the tolerance to the mammalian antigen is in the context of human MHC class II, not mouse MHC class II; (b) contacting said transgenic mouse with said variant antigen; and (c) measuring the immunogenicity of said variant antigen in said transgenic mouse, wherein said mouse MHC class II molecule in said transgenic mouse is deleted or rendered inactive, and wherein said transgenic mouse is not transgenic for human CD4.