Patent ID: 9745618
Date: 2017-08-29
CPC Classifications: C07H,C12Q

Claim:
1. A photoblocked probe comprising: a hydrolysis probe comprising a first nucleic acid sequence complementary to a target comprising a second nucleic acid sequence; a first and a second interactive label; a 5′ end and a 3′ end; and one or more photocleavable moieties coupled to one or more nucleotides of the hydrolysis probe, the photocleavable moiety configured to interfere with hybridization of the first nucleic acid sequence with the second nucleic acid sequence; wherein the photocleavable moiety is attached to the exocyclic amine of adenosine, guanosine and cytidine, and wherein the photocleavable moiety is selected from the group consisting of nitroveratryl, 1-pyrenylmethyl, 2-oxymethylene anthraquinone, 5-bromo-7-nitroindolinyl, 2-nitro-4-bromobenzyl, 2-nitro-4,5-(dimethoxy)benzyl, o-hydroxy-alpha-methyl cinnamoyl, and mixtures thereof.