Patent ID: 9625453
Date: 2017-04-18
CPC Classifications: B01L,G01N

Claim:
1. A microporous membrane comprising a plurality of arrays, each array having capture elements printed on the membrane surface, each capture element corresponding to and being able to bind a target analyte, wherein the capture elements comprise antigens C-reactive protein (CRP), interferon-gamma-inducible protein 10 (IP10) and serum amyloid A (SAA); each array further comprising a plurality of control elements printed on the membrane surface comprising: i) at least two fiduciary markers that permit orientation and gridding of the array, wherein the at least two fiduciary markers comprise hapten conjugated-proteins which bind to target analytes, and wherein the two fiduciary markers are spatially separated by one or more printed elements, ii) at least one negative control to monitor background signal, wherein the at least one negative control comprises a protein, iii) at least one negative control to monitor assay specificity, wherein the at least one negative control comprises a protein, iv) at least one positive colourimetric control, and v) at least one positive control to monitor assay performance, wherein the at least one positive control for assay performance comprises a binding partner corresponding to and able to bind a non-target analyte present in the sample, wherein the microporous membrane is removably attached to a microtiter plate having a plurality of discrete microtiter wells, and wherein each array is discretely disposed in a different microtiter well of the microtiter plate when attached.