Patent ID: 9732326
Date: 2017-08-15
CPC Classifications: A61K,A61P,C12N

Claim:
1. A recombinant replication competent retrovirus comprising: a retroviral GAG protein; a retroviral POL protein; a retroviral envelope; a retroviral RNA polynucleotide comprising 3′ untranslated region (U3) and repeat region (R) sequences at the 3′ end of the retroviral polynucleotide sequence, an R and 5′ untranslated region (U5) sequence at the 5′ end of the retroviral polynucleotide, a gag nucleic acid domain, a pol nucleic acid domain and an env nucleic acid domain located between the U5 and U3 regions; a cassette comprising a pol III promoter upstream and operably linked to a heterologous polynucleotide, wherein the cassette is positioned 5′ to the U3 region and 3′ to the env nucleic acid domain; and cis-acting sequences necessary for reverse transcription, packaging and integration in a target cell; wherein the retroviral RNA polynucleotide provides a proviral DNA sequence comprising a gag nucleic acid domain comprising a sequence from about nucleotide number 1203 to about nucleotide 2819 of SEQ ID NO: 19 or 22, the pol nucleic acid domain comprising the sequence from about nucleotide number 2820 to about nucleotide 6358 of SEQ ID NO:19 or 22, the env domain comprising the sequence from about nucleotide number 6359 to about nucleotide 8323 of SEQ ID NO:19 or 22; and the cassette, and wherein the virus can infect a target cell multiple times resulting in an average number of copies/diploid genome of 5 or greater.