Patent ID: 9708617
Date: 2017-07-18
CPC Classifications: A61P,C12N

Claim:
1. A method for initiating anaerobic-targeted delivery and stable, selective expression, comprising: providing an endostatin gene; isolating a high-effective, anaerobic-specific promoter that initiates anaerobic-specific expression, wherein the high-effective, anaerobic-specific promoter is an alcohol dehydrogenase promoter (padhE), further wherein the padhE promotor contains an active region that initiates anaerobic-specific expression, the active region starting at 500 bp upstream of a target gene of the anaerobic-specific promoter, and the padhE promotor also contains a regulatory region for anaerobic-specific expression starting at 1000 bp-1200 bp upstream of the target gene of the anaerobic-specific promotor; ligating the high-effective, anaerobic-specific promoter together with the endostatin gene, and combining the ligated product into a secretory expression plasmid; transforming the secretory expression plasmid into attenuated prokaryotic cells, wherein the attenuated prokaryotic cells are administering the attenuated prokaryotic cells for treatment of a tumor, wherein the tumor is one of Lewis lung cancer and melanoma, further wherein administration results in anaerobic-targeted delivery and stable, selective expression of the endostatin gene.