Patent ID: 9556488
Date: 2017-01-31
CPC Classifications: C12Q

Claim:
1. A method for sequencing a SCA8 coding sequence in a human subject, comprising: (a) providing a first reaction mixture comprising a DNA molecule in a biological specimen from a human subject and a first primer pair, wherein the DNA molecule comprises a SCA8 coding sequence, and wherein the primers in the first primer pair hybridize to a nucleotide sequence selected from the group consisting of nucleotides 1-448 and nucleotides 726-1159 of SEQ ID NO:1 or complements thereof, nucleotides 1-1070 and nucleotides 1350-1472 of SEQ ID NO:2 or complements thereof, and nucleotides 1-636 and nucleotides 915-1037 of SEQ NO:3 or complements thereof; (b) amplifying the SCA8 coding sequence from the human subject to produce a first amplified DNA fragment; (c) providing a second reaction mixture comprising the first amplified DNA and a second primer pair; (d) amplifying the SCAB coding sequence to produce a second amplified DNA, fragment; and (e) sequencing the second amplified DNA fragment.