Patent ID: 9725694
Date: 2017-08-08
CPC Classifications: C12N

Claim:
1. A method of isolating stem cells, the method comprising contacting said stem cells with a solution comprising a caspase inhibitor, a JNK inhibitor, a protease, and either pyrrolidine dithiocarbamate or clonazepam, wherein the JNK inhibitor is an inhibitor of apoptosis, said JNK inhibitor represented by the structure wherein: A is a direct bond, —(CH R R —(CH —(CH —(CH a is 1, 2, 3, 4, 5 or 6; b and c are the same or different and at each occurrence independently selected from 0, 1, 2, 3 or 4; d is at each occurrence 0, 1 or 2; R R R R or a pharmaceutically acceptable salt, hydrate, solvate, clathrate, enantiomer, diastereomer, racemate, or mixture of stereoisomers thereof; and wherein said method of isolating further comprises isolating the stem cells subsequent to said contacting step; wherein said stem cells are isolated from a mammalian placenta, and said contacting is performed during perfusion of said mammalian placenta with a perfusion solution comprising said caspase inhibitor, said JNK inhibitor, said protease, and said pyrrolidine dithiocarbamate or clonazepam.