Patent ID: 9579421
Date: 2017-02-28
CPC Classifications: A61L

Claim:
1. A method of making a bone graft, the method comprising: (A) obtaining and preparing demineralized cortical fibers by separating cortical bone from bone marrow, rinsing and milling the cortical bone to fibers, demineralizing the cortical fibers, rinsing the cortical fibers in water, and treating the cortical fibers to adjust the pH of the water the fibers are in to between 6.5 and 7 to form the demineralized cortical fibers; (B) obtaining cancellous bone chips from fresh frozen condyles by milling condyles using a bone mill to produce the cancellous bone chips having a size in the range of 0.05 to 1.5 mm and treating by a method selected from the group consisting of: (1) treating the cancellous chips with 0.3-0.5% saline, rinsing the saline treated chips with phosphate buffered saline, mixing the chips with freezing media and dimethyl sulfoxide (DMSO), and freezing the chips in media and storing the chips to form cell enriched cancellous chips; (2) treating the cancellous chips with 0.3-0.5% saline, and rinsing the saline treated chips with phosphate buffered saline; culturing the chips in medium, for up to 10 days; and after the culture period mixing the chips with freezing media and freezing the chips in media and storing the chips to form cell enriched cancellous chips; (3) treating the cancellous chips with collagenase in an incubator for 1-3 hours at 37° C. and 5% O (4) treating the cancellous chips with 0.3-0.5% saline, and then treating and processing the saline treated chips as in (3) to form cell enriched cancellous chips; and (C) mixing the demineralized cortical fibers and cell enriched cancellous chips at a ratio of about 1:1 to 2:1; thereby providing a bone graft material that is osteogenic, osteoinductive and osteoconductive.