Patent ID: 9644235
Date: 2017-05-09
CPC Classifications: C12Q,G01N

Claim:
1. An assay method for detection of a plurality of protein targets in a sample comprising (a) combining target-specific probes with the sample under conditions in which at least 10 target-specific probes each bind a different specific target in the sample, if the specific target is present; wherein each target-specific probe forms a target-specific probe product associated with the target if the target is present in the sample, thereby associating a nucleic acid tag sequence with the target by forming a target-specific probe product associated with the target; wherein the target comprises a protein molecule; (b) segregating aliquots of the sample; and (c) querying each said aliquot for the presence or absence of a different target-specific probe product, wherein said querying comprises amplifying the nucleic acid tag sequence of the probe product using a first primer that binds a first primer binding site of the probe product and a second primer that binds a second primer binding site of the probe product; and detecting the presence or absence of a common nucleic acid tag sequence using a tag-detecting probe; wherein at least two of said target-specific probe products do not have the same first primer binding sites and at least two of said target-specific probe products do not have the same second primer binding sites.