Patent ID: 9708583
Date: 2017-07-18
CPC Classifications: A61K,C07K,C12N,G01N

Claim:
1. A method for stimulating antigen (Ag)-specific T cell responses in a blood sample or an unfractionated peripheral blood mononuclear cell (PBMC) sample isolated from a subject comprising the steps of culturing said blood or unfractionated PBMC sample in an appropriate culture medium which comprises an amount of interleukin-1 beta (IL-1β) and an amount of a least one antigen, wherein the culture medium does not contain a differentiating agent selected from the group consisting of Granulocyte/Macrophage Colony-Stimulating Factor (GM-CSF), interleukin-4 (IL-4) and FMS-like tyrosine kinase 3 (Flt-3) ligand, and detecting amplification of a T cell response to said antigen in said blood sample or said unfractionated PBMC sample.