Patent ID: 9689025
Date: 2017-06-27
CPC Classifications: C12N,C12Q

Claim:
1. A nucleic acid element for use in analysis, the nucleic acid element comprising: a first nucleic acid part; and a second nucleic acid part, wherein the first nucleic acid part is an aptamer that can bind to a subject to be analyzed, wherein the subject to be analyzed is not an enzyme, the second nucleic acid part is a DNAzyme or an RNAzyme that can be detected to distinguish between binding and non-binding of the first nucleic acid part to the subject, a catalytic function of the DNAzyme or the RNAzyme of the second nucleic acid part is inhibited when the subject does not bind to the first nucleic acid part, the inhibition of the catalytic function is removed when the subject binds to the first nucleic acid part, the first nucleic acid part and the second nucleic acid part are linked to each other to form a single-stranded nucleic acid, a part of the first nucleic acid part and a part of the second nucleic acid part associate with each other to form a stem structure in a state where the subject does not bind to the first nucleic acid part, such that the second nucleic acid part is caged by the stem structure and the catalytic function of the DNAzyme or the RNAzyme of the second nucleic acid part is inactivated; the structure of the second nucleic acid part is changed by the change in the structure of the first nucleic acid part, and the second nucleic acid part generates the catalytic function by this change in the structure of the second nucleic acid part; and both the first nucleic acid part and the second nucleic acid part have a secondary structure.