Patent ID: 9707703
Date: 2017-07-18
CPC Classifications: A61L,B29B,C12M,C12N

Claim:
1. A method of producing a biomimetic construct comprising: (i) introducing a gel solution to a well having an opening, (ii) incubating the gel solution to form a gel, (iii) introducing a porous plunger to the well, (iv) compressing the gel with the porous plunger such that liquid is expelled from the gel through a surface of the gel that is contacted by the porous plunger, the liquid, prevented from being expelled through the well bottom, entering the porous plunger and the volume of the gel being reduced, thereby producing the biomimetic construct, wherein a volume of the liquid expelled from the gel into the plunger per unit of surface area of the plunger that contacts the gel is about 2 mm to about 14 mm, and (v) removing the porous plunger containing expelled liquid to leave said biomimetic construct in the well.