Patent ID: 9546356
Date: 2017-01-17
CPC Classifications: C12N,C12P,C12Y,Y02P

Claim:
1. A recombinant, non-xylose fermenting Saccharomyces cerevisiae , which comprises an attenuated phosphofructokinase gene endogenous to said Saccharomyces cerevisiae , an introduced phosphoketolase gene, an introduced Clostridium acetobytylicum acetoacetic acid decarboxylase gene, an Clostridium acetobytylicum butyrate-acetoacetate CoA-transferase subunit A gene, an introduced Clostridium acetobytylicum butyrate-acetoacetate CoA-transferase subunit B gene, an introduced Clostridium acetobytylicum acetyl-CoA acetyltransferase gene, and an introduced Clostridium acetobytylicum isopropanol dehydrogenase gene, wherein isopropanol production by said Saccharomyces cerevisiae is enhanced compared with wild-type strain, and wherein said introduced phosphoketolase gene encodes the amino acid sequence of any one of SEQ ID NOS: 1 to 19.