Patent ID: 9611299
Date: 2017-04-04
CPC Classifications: C07K,C12N,C12P

Claim:
1. A method for expressing a recombinant polypeptide, the method comprising: (1) transfecting a recombinant plasmid capable of expressing a recombinant polypeptide into a recombinant bacteria to obtain a positive monoclonal bacterial colony comprising the recombinant plasmid, wherein the recombinant polypeptide comprises a colicin portion at one end and a target-recognizing portion at the other end, and the colicin portion is hydrophilic and the target-recognizing portion is hydrophobic, and wherein the bacteria is (2) culturing the positive monoclonal bacterial colony in a culture medium to produce a seed bacteria culture of the positive monoclonal bacterial colony, (3) culturing the seed bacteria culture in a growth medium to expand the bacteria culture, (4) adding an inducer not present in the growth medium to induce the bacteria to express the recombinant polypeptide, (5) collecting and breaking down the bacteria to obtain a supernatant containing the expressed recombinant polypeptide, and (6) extracting and purifying the recombinant polypeptide from the supernatant, wherein the growth medium comprises water, NaCl 6.0-6.7 g/L, peptone 25.0 g/L, yeast powder 7.5 g/L, glucose 0.6-2.0 g/L, Na