Patent ID: 9556480
Date: 2017-01-31
CPC Classifications: C12N,C12Q,G01N,G16B

Claim:
1. A method of performing a nucleic acid sequencing reaction, comprising: a) preparing a reaction mixture comprising components of the nucleic acid sequencing reaction including a polynucleotide to be sequenced; b) initiating the nucleic acid sequencing reaction in the reaction mixture to begin progression of the nucleic acid sequencing reaction, wherein the nucleic acid sequencing reaction is initiated during a non-illuminated period, and wherein the reaction mixture comprises an enzyme selected from the group consisting of: a polymerase, a ligase, a ribosome, a nuclease, and a kinase; and c) maintaining conditions that allow the nucleic acid sequencing reaction to proceed while subjecting the reaction mixture to at least one illuminated period following the non-illuminated period, wherein illumination data is collected during the at least one illuminated period, thereby performing the nucleic acid sequencing reaction.