Patent ID: 9534218
Date: 2017-01-03
CPC Classifications: C12N,C12P

Claim:
1. A method for codon optimizing a polynucleotide for the expression of a protein in a bacterial host cell, the method comprising replacing at least one codon in the polynucleotide with a codon that is used more frequently in the genes of a plant than in the genes of the bacterial host cell, such that GC and TA doublets are removed from the polynucleotide sequence; predicted ribonucleic acid (RNA) stem-loop-forming structures are removed from the polynucleotide sequence; at least one restriction enzyme binding sequence is removed from the polynucleotide sequence; sequence blocks that have more than about six consecutive residues of [G+C] or [A+T] are removed from the polynucleotide sequence; and exon:intron junctions, poly-A addition signals, and RNA polymerase termination signals are removed from the polynucleotide sequence.