Patent ID: 9702004
Date: 2017-07-11
CPC Classifications: C12Q

Claim:
1. A method for determining dominant or co-dominant genotypes of one or more polymorphisms, comprising: (a) ligating an adaptor to a nucleic acid fragment to produce an adaptor-ligated nucleic acid fragment; (b) amplifying the adaptor-ligated nucleic acid fragment to produce a library of amplified adaptor-ligated nucleic acid fragments, wherein the amplified adaptor-ligated nucleic acid fragments comprise an identifier tag; (c) sequencing a pool of two or more libraries of amplified adaptor-ligated nucleic acid fragments using high-throughput sequencing, wherein each library comprises a unique identifier tag, wherein the sequencing comprises amplifying by PCR on a solid support; (d) clustering the sequences per library and determining dominant or co-dominant genotypes of one or more polymorphisms in the two or more libraries using the identifier tag.