Patent ID: 9566335
Date: 2017-02-14
CPC Classifications: A61K,C07K,G01N

Claim:
1. A method of sequencing a polypeptide comprising: a. affixing the polypeptide to a substrate; b. contacting the polypeptide with a plurality of probes, wherein each probe comprises an affinity capture reagent that selectively binds to an N-terminal amino acid or a N-terminal amino acid derivative, and a fluorescent label, wherein the affinity capture reagent is a variant ClpS polypeptide comprising an amino acid sequence with at least 70% sequence identity to SEQ ID NO: 1 or 2; c. optically detecting the fluorescent label of the probe bound to the polypeptide molecule, thereby identifying the N-terminal amino acid of the polypeptide; d. cleaving the N-terminal amino acid or N-terminal amino acid derivative of the polypeptide; and e. repeating steps (b) to (d) to determine the sequence of at least a portion of the polypeptide.