Patent ID: 9540426
Date: 2017-01-10
CPC Classifications: C07K,C12N

Claim:
1. A cell culture process for the production of a recombinant CTLA4 molecule, comprising: a) culturing CHO cells which produce a recombinant CTLA4 molecule in cell culture under conditions that allow for protein production; and b) feeding the CHO cells with feeding medium containing dexamethasone, wherein the recombinant CTLA4 molecule comprises the extracellular domain of CTLA4 with the amino acid sequence beginning with methionine at position 27 or alanine at position 26 and ending at aspartic acid at position 150 as shown in SEQ ID NO: 2 joined to an immunoglobulin moiety comprising hinge, CH2 and CH3 domains and wherein the dexamethasone is sustained or maintained in the CHO cell culture at a concentration of 1 nM to 0.1 μM, and wherein the CHO cell culture volume is at least 500 liters.