Patent ID: 9738925
Date: 2017-08-22
CPC Classifications: C12Q

Claim:
1. A method of immobilizing a target nucleic acid to a solid support, said method comprising the steps of: A. mixing a capture oligonucleotide pair containing a first capture and second capture oligonucleotides with a target nucleic acid(s), wherein said capture oligonucleotides each contain a target binding region, a capture region and a stem region, wherein said stem region is located between said target binding and said capture regions, wherein said target binding regions hybridize to adjacent regions of said target nucleic acid, wherein said stem regions are complementary and wherein said capture regions comprise a complementary sequence of a portion of an oligonucleotide bound to said solid support; B. annealing said first capture and second capture oligonucleotides to said target nucleic acid wherein said stem regions of said capture oligonucleotides hybridize bringing said capture regions of said capture oligonucleotides together to produce a combined nucleic acid sequence wherein said combined nucleic acid sequence is complementary to said oligonucleotide bound to said solid support; C. hybridizing said combined nucleic acid sequence to said oligonucleotide bound to said solid support thereby immobilizing said target nucleic acid to said solid support.