Patent ID: 9714947
Date: 2017-07-25
CPC Classifications: G01N

Claim:
1. A cell based sensor that comprises: a. A hematopoietic cell line with regulated exocytosis of secretory granules; b. A granule stored non-protease hydrolase reporter transfected into the cell line of (a) and overexpressed under the control of either a strong constitutive promoter or a strong inducible promoter; c. A modulator of regulated exocytosis of the secretory granules of the cell line of (a), wherein the modulator is an endogenous surface receptor or a transfected heterologous surface receptor, wherein the modulator is selected from the group consisting of: G-protein coupled receptors (GPCR), receptors bearing an ITAM motif, receptors bearing an ITIM motif, and protein tyrosine kinases receptors; d. A cell impermeable substrate selected from the group comprising: a colorimetric, a fluorescent or a luminescent substrate specific for detection of a secreted non-protease hydrolase activity; which allows measuring the effect of a specific ligand on the modulator of regulated exocytosis.