Patent ID: 9588117
Date: 2017-03-07
CPC Classifications: G01N

Claim:
1. A method for detecting production of an antigen-specific antibody, the method comprising: tagging an antibody-producing cell with a cross-linking reagent, wherein the cross-linking reagent comprises a first portion adapted to bind to a cell surface marker that is specific to the antibody-producing cell and a second portion that comprises a protein of interest, wherein the antigen-specific antibody is specific to the protein of interest; allowing the antibody-producing cell to produce antigen-specific antibody, such that the antigen-specific antibody binds to the protein of interest; and exposing the antibody-producing cell to a labeled secondary antibody configured to specifically bind to the antigen-specific antibody; and detecting whether the antibody-producing cell is bound to the labeled secondary antibody.