Patent ID: 9606082
Date: 2017-03-28
CPC Classifications: B01L,B05B,G01N,H01J

Claim:
1. A method of sample processing, comprising: providing a microfluidic device with at least one separation channel in fluid communication with a background electrolyte (BGE) reservoir, a waste reservoir having a waste channel that merges into the separation channel, and a sample reservoir having a sample channel that merges into the separation channel; injecting a fluid sample from the sample reservoir into the separation channel downstream of the BGE reservoir by concurrently applying defined pressures to the BGE reservoir and the sample reservoir; then clearing a trailing end of the sample from the sample channel and flowing fluid from the BGE reservoir to deliver a plug of the sample in the separation channel in response to reducing or removing the pressure applied to the sample reservoir while applying pressure to the BGE reservoir so that pressure applied to the BGE reservoir is greater than pressure then applied to the sample reservoir; and then electrophoretically separating the delivered sample in the separation channel by applying voltage to the microfluidic device.