Patent ID: 9707279
Date: 2017-07-18
CPC Classifications: A61K,A61P,G01N

Claim:
1. A method of treating a disease or condition mediated by pathogenic IgE antibodies in a subject, the method comprising: (a) selecting a subject having a disease or condition characterized by one or more of (i) undesirable IgE production, (ii) excessive, harmful or unwanted IgE-mediated basophil activation, and (iii) excessive, harmful or unwanted IgE-mediated mast cell activation, wherein the disease or condition is selected from a hyper-IgE syndrome, an atopic disorder, an allergic reaction, and a hypersensitivity reaction characterized by having an abnormally increased amount of IgE specific for one or more allergens; and (b) administering to the subject a therapeutically effective amount of an EndoS polypeptide to reduce interactions between the pathogenic IgE antibodies and FcεR on FcεR bearing cells in the subject, relative to said interactions prior to said step of administering, and thereby treating the disease or condition mediated by pathogenic IgE antibodies, wherein the EndoS polypeptide comprises: (i) the amino acid sequence of SEQ ID NO: 1, (ii) a fragment of (i) having IgE endoglycosidase activity which hydrolyzes at least one IgE Cε asparagine-linked glycan between two core N-acetylglucosamine residues of the glycan, (iii) a variant of (i) having at least 97% identity to the amino acid sequence of SEQ ID NO: 1 and having IgE endoglycosidase activity which hydrolyzes at least one IgE Cε asparagine-linked glycan between two core N-acetylglucosamine residues of the glycan; or (iv) a variant of (ii) having at least 97% identity to a corresponding portion of the amino acid sequence of SEQ ID NO: 1 and having IgE endoglycosidase activity which hydrolyzes at least one IgE Cε asparagine-linked glycan between two core N-acetylglucosamine residues of the glycan.