Patent ID: 9671410
Date: 2017-06-06
CPC Classifications: A61K,A61Q,G01N

Claim:
1. A screening method for identifying a body wash composition as effective at improving the health of human skin, comprising: a. during a treatment period comprising at least one treatment, contacting a skin surface of a human subject with a body wash composition during a treatment period, wherein the body wash composition is washed off after each application; b. at least once during the treatment period extracting from the epidermis of the human subject (i) at least one biomarker selected from the group consisting of IL1 rα and IL 1α, (ii) at least one biomarker selected from the group consisting of Trans-Urocanic Acid, Citrulline, Glycine, Histidine, Ornithine, Proline, 2 Pyrrolidone 5 acid, and Serine,(iii) at least one biomarker that is a ceramide, (iv) at least one biomarker that is a fatty acid, and (v) total protein; c. measuring an amount of each biomarker extracted; and d. identifying the body wash composition as effective if the amount of each biomarker is shifted in a direction of improved skin health with total protein decreasing.