Patent ID: 9593307
Date: 2017-03-14
CPC Classifications: C12N

Claim:
1. A method for expanding human pluripotent stem cells comprising culturing the human pluripotent stem cells on a feeder-free matrix in a defined cell culture formulation, wherein the defined cell culture formulation consists essentially of DMEM-F12 basal medium, insulin, transferrin, selenium, fatty-acid free albumin, from about 0.5 ng/ml to about 10 ng/ml of a TGF-β ligand, from about 50 ng/ml to about 100 ng/ml of bFGF, and from about 10 ng/ml to about 50 ng/ml of IGF-1, and wherein culturing the human pluripotent stem cells in the defined cell culture formulation maintains the pluripotency and karyotypic stability of the cells for at least 10 passages.