Patent ID: 9573990
Date: 2017-02-21
CPC Classifications: C07K

Claim:
1. A method for producing a plurality of isolated antibodies for profiling antigen expression comprising (a) binding, in vitro, an isolated plurality of antibody-producing B-cells to an isolated plurality of cognate antigens from a mammal; (b) isolating each cognate antigen-bound antibody-producing B-cell; (c) amplifying each nucleic acid sequence encoding each antibody, or fragment thereof, from each B-cell; (d) introducing each amplified nucleic acid sequence encoding each antibody, or fragment thereof, into an expression system; (e) expressing and purifying each antibody, or fragment thereof; and (f) preparing an array consisting of each purified antibody, or fragment thereof, from step (e) on a substrate for profiling antigen expression.