Patent ID: 9556443
Date: 2017-01-31
CPC Classifications: C12N,C12P,Y02P

Claim:
1. A genetically engineered yeast cell in which the activity of a protein having a sequence identity of about 95% or more to an external mitochondrial NADH dehydrogenase of SEQ ID NO: 1 or 2 is decreased compared to a parent cell of the genetically engineered yeast cell, wherein the genetically engineered yeast cell produces lactate, a gene encoding the external mitochondrial NADH dehydrogenase is inactivated or deleted in the genetically engineered yeast cell, and the yeast cell comprises a heterologous gene encoding a polypeptide that converts pyruvate to lactate, such that the activity of the polypeptide that converts pyruvate to lactate in the genetically engineered yeast cell is increased as compared to the parent cell.