Patent ID: 9650681
Date: 2017-05-16
CPC Classifications: C12Q

Claim:
1. A composition for the detection and/or identification of CTX-M extended spectrum β-lactamase sequences comprising CTX-M isoforms 1-82 in a specimen, comprising: a set of amplification primers in solution, wherein the set of amplification primers comprises a plurality of amplification primer pairs comprising first and second primers, wherein said plurality of amplification primer pairs is collectively 100% complementary to each of CTX-M isoforms 1-82, and hybridizes to and produces target amplification products of any of CTX-M isoforms 1-82 when said any of CTX-M isoforms 1-82 are present in a specimen under conditions sufficient to provide polymerase-based nucleic acid-based amplification, wherein at least one primer comprises a nucleotide sequence selected from the group consisting of SEQ ID NOs: 1-4, 6, 7, 11, 13-16, 18-25, and 27-28, comprising at least one substitution of a naturally occurring nucleotide with a nucleotide analog that can hybridize to more than one nucleotide selected from A, T, C, and G; wherein said first and second primers in each amplification primer pair in said plurality of amplification primer pairs are 12 to 45 bases in length and comprise at least 12 consecutive nucleotides of at least one primer pair selected from the group consisting of: SEQ ID NOs: 1 and 2 or the complements thereof; SEQ ID NOs: 3 and 4 or the complements thereof; SEQ ID NOs: 6 and 7 or the complements thereof; SEQ ID NOs: 27 and 28 or the complements thereof; SEQ ID NOs: 8 and 9 or the complements thereof; and SEQ ID NOs: 29 and 30 or the complements thereof, and wherein said first and second primers in each amplification primer pair in said plurality of amplification primer pairs are 100% complementary to at least one of SEQ ID NOs: 48-51 or the complement thereof.