Patent ID: 9672331
Date: 2017-06-06
CPC Classifications: A23K,G01N,G16C,G16H

Claim:
1. A method for analyzing feed for swine or poultry comprising steps of: (a) digesting in vitro a plurality of samples of animal feed each having an animal feed composition to generate a plurality of digested animal feed samples, wherein each of the plurality of digested animal feed samples comprises at least one residual component, wherein the at least one residual component is selected from the group consisting of protein, phosphorous, fat, gross energy, and carbohydrates, wherein the samples of animal feed consist of swine feed or poultry feed, wherein each animal feed composition comprises one or more of corn, soybean, grain(s), distiller grain(s), legumes, molasses, or a mixture of any thereof, wherein the digesting in vitro comprises at least two phases: wherein for swine feed an in vitro digestion phase is conducted for 1 to 6 hours at a pH of less than about 7 and includes use of at least one enzyme comprising pepsin, and wherein another in vitro digestion phase is conducted for 18 hours to 48 hours at neutral pH to an acidic pH of at least 6.0 and includes use of at least one enzyme comprising pancreatin, to generate the plurality of digested animal feed samples, wherein for poultry feed, an in vitro digestion phase is conducted that includes use of at least one enzyme comprising phytase, another in vitro digestion phase is conducted at a pH of less than about 7 for 30 minutes to 2 hours and includes use of at least one enzyme comprising pepsin, and an additional in vitro digestion phase is conducted at neutral pH to an acidic pH of at least 6.0 for 30 minutes to 2 hours and includes use of at least one enzyme comprising pancreatin, to generate the plurality of digested animal feed samples; (b) separating each of the plurality of digested animal feed samples obtained in step (a) into a solid component and a liquid component; (c) scanning the solid component of each of the plurality of digested animal feed samples obtained in step (b) using a near infrared (NIR) spectrophotometer to generate spectral data for each of the plurality of digested animal feed samples; (d) determining the concentration of the at least one residual component in each of the plurality of digested animal feed samples using a wet chemistry method; (e) generating a computer model comprising a computer implemented method comprising steps of: (f) validating the computer model for analyzing feed for swine or poultry using validation samples, wherein the validation samples comprise a plurality of different samples of the animal feed that are different from the samples used in step (a), comprising comparing a concentration of at least one residual component in each of a plurality of digested samples obtained from the different samples of the animal feed that is predicted from the computer model to a corresponding measured concentration determined by wet chemistry for the at least one residual component in each of the plurality of digested samples obtained from the different samples of the animal feed.