Patent ID: 9637764
Date: 2017-05-02
CPC Classifications: C12N,C12P,C12Y

Claim:
1. A method for biosynthesizing a product selected from the group consisting of pimelic acid, 7-aminoheptanoic acid, 7-hydroxyheptanoic acid, heptamethylenediamine, and 1,7-heptanediol, said method comprising: (i) contacting 2-oxoglutarate with enzymes comprising a homocitrate synthase classified under EC 2.3.3.14 or EC 2.3.3.13, a homocitrate dehydratase and a homoaconitate hydratase classified under EC 4.2.1.114, EC 4.2.1.36 or EC 4.2.1.33 an iso(homo) contacting acetyl-CoA with enzymes comprising a β-ketothiolase classified under EC 2.3.1.9, an acetyl-CoA carboxylase classified under EC 6.4.1.2, an acetoacetyl-CoA synthase classified under EC 2.3.1.194, a 3-hydroxyacyl-CoA dehydrogenase classified under EC 1.1.1.-, an enoyl-CoA reductase classified under 4.2.1.-, a glutaconyl-CoA decarboxylase classified under EC 4.1.1.70, and an enoyl-[acp] reductase classified under EC 1.3.1.9 or a trans-2-enoyl-CoA reductase classified under EC 1.3.1.44 or a glutaryl-CoA dehydrogenase to produce glutaryl-CoA; (ii) contacting said glutaryl-CoA with enzymes comprising a β-ketothiolase classified under EC 2.3.1.- or a β-ketoacyl-[acp] synthase classified under EC 2.3.1.41, EC 2.3.1.179, EC 2.3.1.180, a 3-hydroxyadipyl-CoA dehydrogenase classified under EC 1.1.1.157, an enoyl-CoA hydratase, and an enoyl-[acp] reductase classified under EC 1.3.1.9 or a trans-2-enoyl-CoA reductase classified under EC 1.3.1.44 to produce pimeloyl-CoA, or contacting said glutaryl-CoA with enzymes comprising a β-ketothiolase classified under EC 2.3.1.- or a β-ketoacyl-[acp] synthase classified under EC 2.3.1.41, EC 2.3.1.179, EC 2.3.1.180, a 6-oxo-cyclohex-1-ene-carbonyl-CoA hydrolase classified under EC 3.7.1.-, a 6-hydroxycyclohex-1-ene-1-carboxyl-CoA dehydrogenase classified under EC 1.1.1.-, a cyclohexa-1,5-dienecarbonyl-CoA hydratase classified under EC 4.2.1.100, a cyclohex-1-ene-1-carboxyl-CoA dehydrogenase classified under EC 1.3.8.-, a cyclohex-1-ene-1-carboxyl-CoA hydratase classified under EC 4.2.1-, a 2-hydroxycyclohexanecarboxyl-CoA dehydrogenase classified under EC 1.1.1.-, and a 2-ketocyclohexanecarboxyl-CoA hydrolase classified under EC 4.1.3.36 to produce pimeloyl-CoA; (iii) contacting said pimeloyl-CoA with enzymes comprising a thioesterase classified under EC 3.1.2., an aldehyde dehydrogenase classified under EC 1.2.1.3, a 6-oxohexanoate dehydrogenase classified under EC 1.2.1.63, a 7-oxoheptanoate dehydrogenase classified under EC 1.2.1.-, a glutaconate-CoA transferase classified under EC 2.8.3.12, or a reversible CoA-ligase classified under EC 6.2.1.5 to produce pimelic acid or 7-hydroxyheptanoate, and/or contacting said pimeloyl-CoA with enzymes comprising an ω-transaminase classified under EC 2.6.1.- or a deacetylase classified under 3.5.1.62 to produce 7-aminoheptanoate or heptamethylenediamine; and (iv) optionally, contacting said 7-hydroxyheptanoate from step (iii) with an alcohol dehydrogenase classified under EC 1.1.1.- to produce 1,7-heptanediol; wherein at least one of said enzymes is a heterologous enzyme.