Patent ID: 9630177
Date: 2017-04-25
CPC Classifications: B01F,B01L,C12N,C12Q,F16K

Claim:
1. A microfluidic device, comprising: a first channel having a sequence of (n) chambers each having a first volume (v), wherein said first channel comprises valves at opposing ends of said first channel that fluidically isolate said first channel; and a second channel in fluid communication with said first channel, wherein said second channel includes at least one second chamber having a total second volume that is at least equal to said total volume of said first channel (n*v), wherein said second channel comprises valves at opposing ends of said second channel that fluidically isolate said second channel from said first channel; and at least a fluidic layer, an actuation layer, and an elastic layer sandwiched between said fluidic layer and said actuation layer, wherein at least one of said valves comprises: a) a diaphragm in said elastic layer; b) a valve seat that selectively isolates an inlet and an outlet of said at least one of said valves; c) a chamber in said actuation layer, which chamber supplies positive or negative fluidic or pneumatic pressure to move said diaphragm towards or away from said valve seat, and d) a pin that is movable within said chamber relative to said diaphragm, wherein said diaphragm is movable towards said valve seat using positive pressure at least partly supplied by said pin.