Patent ID: 9591835
Date: 2017-03-14
CPC Classifications: A01K

Claim:
1. A mouse whose genome comprises at an endogenous H-2D locus a nucleotide sequence encoding a chimeric human/mouse HLA-B/H-2D polypeptide, wherein the nucleotide sequence is in operable linkage with an endogenous mouse regulatory element and operably linked to a MHC I polypeptide leader encoding sequence, wherein the nucleotide sequence comprises from 5′ to 3′ (i) a first nucleic acid sequence that encodes the α1, α2 and α3 domains of the human HLA-B polypeptide, which first nucleic acid sequence replaces a sequence encoding the α1, α2 and α3 domains of the mouse H-2D polypeptide at the endogenous H-2D locus, and (ii) a second nucleic acid sequence that encodes the transmembrane and cytoplasmic domains of the mouse H-2D polypeptide, wherein the mouse expresses, on the surface of nucleated cells, from the endogenous H-2D locus the chimeric HLA-B/H-2D polypeptide that comprises the α1, α2 and α3 domains of the human HLA-B polypeptide and transmembrane and cytoplasmic domains of the mouse H-2K polypeptide.