Patent ID: 9594941
Date: 2017-03-14
CPC Classifications: G01N,G02B,G06K

Claim:
1. A phase distribution measurement method a for biological sample, comprising: taking in an optical image of a biological sample formed by a microscope that converts phase distribution into image intensity distribution while changing image contrast to form a plurality of pieces of images with different image contrasts; calculating a component corresponding to phase distribution of the biological sample and a component corresponding to other than the phase distribution of the biological sample based on the plurality of pieces of images, and dividing the component corresponding to the phase distribution by the component corresponding to other than the phase distribution of the biological sample to form a normalized phase component image; breaking down the normalized phase component image into a plurality of frequency components based on a spatial frequency of the normalized phase component image; performing a deconvolution process to each of the frequency components using an optical response character corresponding to each, and calculating phase distribution of a refraction component formed by light refracted inside the biological sample and phase distribution of a structure component formed by light diffracted in a structure inside the biological sample; and calculating phase distribution of the biological sample by compounding the phase distribution of the refraction component and the phase distribution of the structure component.