Patent ID: 9695466
Date: 2017-07-04
CPC Classifications: C12Q

Claim:
1. A method of generating one or more copies of a target DNA molecule, or part thereof, that contains a repeat of identical nucleotides, comprising the steps of: altering one nucleotide in this repeat of identical nucleotides, or altering different single nucleotides separated at intervals in said repeat of identical nucleotides into another nucleotide, in order to reduce said repeat of identical nucleotides into two or more smaller altered parts of said identical nucleotides in the copied molecules, wherein the alteration is performed by amplification with an oligonucleotide primer comprising at the 5′ side a sequence which hybridizes with the sequence preceding the targeted sequence repeat, and comprising at the 3′ side a sequence which hybridizes with the targeted sequence repeat, wherein the sequence which hybridizes with the targeted sequence repeat is not 100% complementary with said repeat.