Patent ID: 9726667
Date: 2017-08-08
CPC Classifications: G01N

Claim:
1. A method to identify the probable status of tuberculosis infection in a subject which method comprises (a) preparing a tryptic digest of proteins in a sample of a biological fluid from said subject, wherein said tryptic digest may include at least one tryptic peptide selected from the group consisting of AESDDLDALVAHLPADR (SEQ ID NO:1); AAPATVSELDR (SEQ ID NO:2); ALEIVDALPR (SEQ ID NO:3); MCVLTGK (SEQ ID NO:4); NFYDADPLAK (SEQ ID NO:5); VELDDLLHK (SEQ ID NO:6); FSIGTNTIGTESR (SEQ ID NO:7); DSVDDIR (SEQ ID NO:8); AFEAGEPQASGK (SEQ ID NO:9); VYTTTPK (SEQ ID NO:10); DIILAPVISEK (SEQ ID NO:11); LGEFAPTR (SEQ ID NO:12); ATEYPSAVAK (SEQ ID NO:13); EELFNLR (SEQ ID NO:14); DWLVSAR (SEQ ID NO:15); VAGCDQAAVYAPK (SEQ ID NO:16); LSDSPLQVTIWTK (SEQ ID NO:17); ELAGTFSAR (SEQ ID NO:18); VSAELITAAR (SEQ ID NO:19); SFCALGDGAASPVMSSIK (SEQ ID NO:20); VNIKPLEDK (SEQ ID NO:21); and MFYNQYR (SEQ ID NO:22); (b) contracting said digest with at least one affinity reagent that binds at least one said peptide, (c) detecting the presence or absence of a complex of any said tryptic peptide in said digest formed by reaction with the affinity reagent; thereby detecting the presence or absence and/or quantity of the peptide in said digest; whereby the probable status of tuberculosis infection in said subject is identified.