Patent ID: 9587233
Date: 2017-03-07
CPC Classifications: A61K,A61P,C12N,C12Y

Claim:
1. An isolated two-chain polypeptide comprising: a first chain comprising amino acid residues 95-105 of SEQ ID NO: 20 or a first amino acid sequence having at least 95% sequence identity to amino acid residues 95-105 of SEQ ID NO: 20 and a second chain comprising amino acid residues 112-346 of SEQ ID NO: 20 or a second amino acid sequence having at least 95% sequence identity to amino acid residues 112-346 of SEQ ID NO: 20, wherein the polypeptide (a) has reduced catalytic activity as compared to the corresponding wild-type factor Xa protein, (b) is capable of binding to a factor Xa inhibitor and (c) cannot assemble into a prothrombinase complex, and wherein (i) the first chain does not include amino acid residues 12-50 of SEQ ID NO: 20, (ii) the first chain does not include amino acid residues 51-94 of SEQ ID NO: 20, or (iii) the second chain does not include amino acid residues 347-365 of SEQ ID NO: 6.