Patent ID: 9687531
Date: 2017-06-27
CPC Classifications: A61K,C07H,C07K,C12N,C12P,C12Y

Claim:
1. A method of coupling a beta-glucocerebrosidase to an oligosaccharide that comprises a phosphorylated mannose, comprising: (a) derivatizing an oligosaccharide comprising a phosphorylated mannose with a compound containing a carbonyl-reactive group; (b) oxidizing a beta-glucocerebrosidase having at least one oligosaccharide to generate at least one carbonyl group on the at least one oligosaccharide of the beta-glucocerebrosidase; and (c) reacting the derivatized oligosaccharide with the oxidized beta-glucocerebrosidase, thereby coupling the oligosaccharide to the beta-glucocerebrosidase.