Patent ID: 9624487
Date: 2017-04-18
CPC Classifications: B01D,C12N

Claim:
1. A nucleic acid purification kit comprising: a disposable cartridge having a layered configuration, the disposable cartridge comprising a first surface of a first layer of the disposable cartridge and an access port on the first surface, and each of a plurality of layers of the disposable cartridge being joined to an adjacent layer by a corresponding adhesive material layer; a first fluid pouch and a second fluid pouch coupled to the first surface of the disposable cartridge, wherein the first fluid pouch comprises a first air vent configured to be opened for releasing a stored first reagent from the first fluid pouch into the disposable cartridge and the second fluid pouch comprises a second air vent configured to be opened for releasing a stored second reagent from the second fluid pouch into the disposable cartridge; and a disposable silica-containing compartment for capturing the nucleic acid, wherein the disposable silica-containing compartment has a distal opening and a proximal opening, the distal opening of the disposable silica-containing compartment being configured to couple to the access port on the first surface of the disposable cartridge, the silica-containing compartment being operably connected to the disposable cartridge, the proximal opening configured to be coupled to a device configured to perform one or more of: delivering fluid into the disposable cartridge and withdrawing fluid out of the disposable cartridge; wherein the disposable cartridge comprises a first layer comprising a first cut-out portion for forming a first fluid chamber in fluid communication with the first fluid pouch, the first fluid pouch and the first fluid chamber being in fluid communication with the access port and being configured for receiving and storing a discrete quantity of the first reagent, wherein the first reagent is subsequently released from the first fluid pouch and the first fluid chamber to contact silica of the disposable silica-containing compartment, wherein the disposable cartridge comprises a second layer comprising a second cut-out portion for forming a second fluid chamber in fluid communication with the second fluid pouch, the second fluid pouch and the second fluid chamber being in fluid communication with the access port and being configured for receiving and storing a discrete quantity of the second reagent, wherein the second reagent is subsequently released from the second fluid pouch and the second fluid chamber to contact silica of the disposable silica-containing compartment, wherein the disposable cartridge comprises at least one additional layer comprising a fluid channel cut-out portion for forming a fluid channel to fluidly connect at least one of the first fluid chamber and the second fluid chamber to the access port on the first surface of the disposable cartridge, and wherein each of the first layer, the second layer and the at least one additional layer comprises on a surface the corresponding adhesive material layer, the corresponding adhesive material layer comprising a respective cut-out portion corresponding to the first fluid chamber, the second fluid chamber or the fluid channel.