Patent ID: 9738719
Date: 2017-08-22
CPC Classifications: C07K,C40B,G01N

Claim:
1. A method for identifying a modulator antibody of a phenotype of a eukaryotic cell type, comprising (a) expressing in a population of cells of the eukaryotic cell type by transducing a library of either secreted candidate antibodies or antigen-binding fragments thereof to produce a heterogeneous population of modified, antibody-expressing cells, wherein the transduced cells are plated in a diffusion restricting medium such that the secreted antibodies are trapped around the cells producing the secreted antibodies or membrane-integrated candidate antibodies or antigen-binding fragments thereof to produce a heterogeneous population of modified, antibody-expressing cells, wherein the entire membrane-integrated antibody library and its target receptor are co-integrated into the plasma membranes of said population of cells of the eukaryotic cell type, and (b) selecting, in an autocrine manner, a specific antibody-expressing cell with an altered phenotype relative to that of an unmodified control cell of said eukaryotic cell type; thereby identifying the candidate antibody expressed in the specific antibody-expressing cell as a modulator of the eukaryotic cell type.