Patent ID: 9683960
Date: 2017-06-20
CPC Classifications: C12Q,G01N

Claim:
1. A method for sequence-specifically detecting a nucleic acid molecule, comprising the consecutive steps of: a) contacting in an absence of an electric field, a mixture of nucleic acid molecules with a labeled peptide nucleic acid (PNA) having a sequence of interest in a first solution and obtaining a hybrid consisting of said nucleic acid molecule and said PNA; b) introducing said first solution comprising said hybrid resulting from step (a) into an isotachophoresis (ITP) system, said ITP system comprises a second solution of high effective mobility leading electrolyte (LE) ions and a third solution of low effective mobility trailing electrolyte (TE); c) applying said electric field across said second solution and said third solution; wherein said hybrid but not free PNA focus at the sharp LE-TE interface in said ITP system, wherein said TE has a higher mobility than the PNA molecule and said TE has a lower mobility than said hybrid, thereby sequence-specifically said detecting said nucleic acid molecule by a signal from a label.