Patent ID: 9545064
Date: 2017-01-17
CPC Classifications: A01H

Claim:
1. A tissue culturing method of Davalliaceae family ferns, comprising following steps: providing a chopped gametophyte of Davalliaceae family ferns; generating a callus, by shaking culturing the chopped gametophyte of Davalliaceae family ferns in a liquid medium which comprises ½ MS salts and 20 g/L sucrose, till the chopped gametophyte of Davalliaceae family ferns generates the callus; and performing apogamic regeneration of a sporophyte, by shaking culturing the calluses in a culture fluid which comprises ½ MS salts, 20 g/L sucrose, and 1-2 mg/L N6-(delta 2-isopentenyl)-adenine (2ip), to develop the sporophyte from the callus.