Patent ID: 9551022
Date: 2017-01-24
CPC Classifications: C12Q

Claim:
1. A method for measuring a substance in a blood sample by an enzymatic method, the method comprising: (1) contacting the blood sample with a non-ionic surfactant; (2) then contacting a sample contacted with the non-ionic surfactant from (1) with a betaine-type amphoteric surfactant, and (3) performing an enzymatic reaction and a color reaction with an oxidizable color reagent at the same time as the contact or after the contact with the betaine-type amphoteric surfactant, wherein the non-ionic surfactant is at least one surfactant selected from the group consisting of a polyoxyethylene polyoxypropylene condensate, polyoxyethylene polyoxypropylene alkyl amine condensate, polyoxyethylene polyoxypropylene diamine condensate, polyoxyethylene alkyl ether, polyoxyethylene alkyl phenyl ether, and polyoxyethylene polyalcohol fatty acid ester, and the amount of the non-ionic surfactant is from 0.1 to 10 w/v %, and wherein the betaine-type amphoteric surfactant is at least one surfactant selected from the group consisting of alkyl betaine and amidoalkyl betaine, and the amount of the betaine-type amphoteric surfactant is from 0.5 to 10 w/v %, wherein the blood sample is plasma or serum, and wherein hemoglobin and bilirubin are highly likely to affect a measurement value of the substance that is measured in the blood sample, and wherein the substance in the blood sample is measured by quantifying hydrogen peroxide produced by the enzyme reaction, with the proviso that the substance to be measured is not hemoglobin nor bilirubin.