Patent ID: 9695428
Date: 2017-07-04
CPC Classifications: A61L,C12M,C12N,C12Q,C12Y

Claim:
1. A composition, comprising: a host organism comprising a spore forming bacteria selected from the group consisting of Geobacillus stearothermophilus, Bacillus atrophaeus, Bacillus sphaericus, Bacillus anthracis, Bacillus pumilus, Bacillus coagulans, Bacillus subtilis globigii, Bacillus cereus or Bacillus circulans; a reporter gene for producing an indicator enzyme; a regulatory gene; and a vehicle for inserting the reporter gene and the regulatory gene in the host organism; the host organism bearing a transposable genetic element in its genome for inserting an insertion sequence in the regulatory gene, wherein the insertion sequence comprising an IS21 family insertion sequence selected from the group consisting of IS21, IS53, IS232A, IS408, IS1162, IS1326, IS1415 or IS5376, wherein the vehicle is taken up by the host organism, the insertion sequence is inserted in the regulatory gene, and the host organism undergoes sporulation.