Patent ID: 9677105
Date: 2017-06-13
CPC Classifications: C07K,C12P

Claim:
1. A method of producing an adalimumab preparation having a target value of one or more of high mannose glycans and fucosylated glycans, the method comprising: (a) providing a cell genetically engineered to express adalimumab; (b) culturing the cell in a culture medium comprising 1 mM to 50 mM ammonium and 1 g/L to 30 g/L lysine under conditions in which the cell expresses the adalimumab; and (c) harvesting a preparation of the adalimumab produced by the cell that meets-the target value of one or more of high mannose glycans and fucosylated glycans; wherein (i) the target value of high mannose glycans is a level that is at least 10% higher than a level of high mannose glycans in a preparation of adalimumab produced by culturing the cell in the medium not comprising 1 mM to 50 mM ammonium and 1 g/L to 30 g/L lysine, and (ii) the target value of fucosylated glycans is a level that is at least 10% lower than a level of fucosylated glycans in a preparation of adalimumab produced by culturing the cell in the medium not comprising 1 mM to 50 mM ammonium and 1 g/L to 30 g/L lysine.