Patent ID: 9599610
Date: 2017-03-21
CPC Classifications: A23C,A61K,C12N,C12Q,G01N

Claim:
1. A method for isolating a pathogen from a sample, the method comprising removably coupling a vessel comprising a pathogen-containing sample to a cartridge via a cartridge interface that is configured to provide communication between the vessel and the cartridge; flowing magnetic particles conjugated to a capture moiety from the cartridge into the vessel to form a mixture; flowing the mixture from the vessel into the cartridge; flowing a fluid from the cartridge into the vessel to flush residual mixture from the vessel into the cartridge; and incubating the mixture in the cartridge to allow the particles to bind to the pathogen; applying a magnetic field to capture pathogen/magnetic particle complexes on a surface of a first magnetic trap; washing the captured pathogen/magnetic particle complexes with a wash solution, thereby isolating the pathogen/magnetic particle complexes; removing the magnetic field; re-introducing the wash solution, thereby re-suspending the pathogen/magnetic particle complexes; flowing the re-suspended pathogen/magnetic particle complexes through a channel and into a second magnetic trap; and engaging the second magnetic trap, thereby re-capturing the pathogen/magnetic particle complexes; wherein the first magnetic trap is configured to capture the pathogen/magnetic particle complexes from a macro-scale volume of fluid, and the second magnetic trap is configured to concentrate substantially all of the pathogen/magnetic particle complexes into a micro-scale volume of fluid.