Patent ID: 9586987
Date: 2017-03-07
CPC Classifications: C07H,C12Q

Claim:
1. A primer set for use in a method for isothermally amplifying a target nucleic acid sequence, the primer set comprising: a first primer; and a second primer, wherein the first primer comprises, on the 3′ side thereof, a sequence (A′) that can hybridize to a sequence (A) on the 3′ side of the target nucleic acid sequence, the second primer comprises, on the 3′ side thereof, a sequence (B′) that can hybridize to a sequence (B) on the 3′ side of either a strand extended from the first primer or a strand complementary to the target nucleic acid sequence, and the first primer and the second primer comprise, on the 5′ sides thereof, sequences (C) that are substantially identical to each other, wherein at least one of the first primer and the second primer further comprises, on the 5′ side of the sequence (C), a folding sequence (D-D′) comprising, on the same strand, two sequences that hybridize to each other.