Patent ID: 9556466
Date: 2017-01-31
CPC Classifications: C12N,C12P,C12Q

Claim:
1. A method for one-step reverse transcriptase-polymerase chain reaction (RT-PCR), said method comprising: (a) forming a mixture by combining an RNA template with a composition comprising a Moloney murine leukemia virus (M-MLV) reverse transcriptase and one or more DNA polymerases, and bovine serum albumin (BSA), wherein the unit ratio of said M-MLV to said one or more DNA polymerases is greater than 3:2 and wherein said BSA is at a concentration of about 2 to 20 μg/mL; (b) incubating the mixture under conditions sufficient to amplify a DNA molecule complementary to all or a portion of said RNA template in which said BSA is capable of relieving inhibition of said RT-PCR by said M-MLV.