Patent ID: 9677143
Date: 2017-06-13
CPC Classifications: A61P,C12N,C12Q

Claim:
1. A method of identifying an anti-HIV agent, comprising: forming a mixture comprising a linear sequence of a tRNA anticodon stem loop fragment that is not capable of forming a stem-loop, a target nucleic acid molecule capable of binding to the tRNA anticodon stem loop fragment, and a test compound, wherein the target nucleic acid molecule comprises residues 157-169 of the 5′-untranslated region of HIV 1; incubating the mixture under conditions that allow binding of the tRNA anticodon stem loop fragment and the target nucleic acid molecule in the absence of the test compound; and detecting whether or not the test compound inhibits the binding of the tRNA anticodon stem loop fragment and the target nucleic acid molecule, wherein the absence of binding of the tRNA ASL fragment and the target nucleic acid molecule is indicative of the test compound being: i) an inhibitor of retroviral propagation, wherein the tRNA anticodon stem loop fragment optionally comprises a label.