Patent ID: 9556433
Date: 2017-01-31
CPC Classifications: C12N

Claim:
1. A recombinant nucleic acid molecule comprising from 5′ to 3′ and in the order from (a)-(g): (a) a flanking sequence; (b) a first lower stem sequence which is 12, 13, 14, 15 or 16 nucleotides in length; (c) an anti-sense target sequence 22 nucleotides in length; (d) a loop sequence; (e) a sense sequence wherein the sequence is complementary to the sequence of (c) except that the sequence comprises one or two mismatches relative to the sequence of (c), wherein the one or two mismatches comprise: (f) a second lower stem sequence wherein the sequence is complementary to the sequence of (b); and (g) a second flanking sequence.