Patent ID: 9745351
Date: 2017-08-29
CPC Classifications: A61K,A61P,C07K,C12N,C12P,G01N

Claim:
1. A method for producing a human papillomavirus (HPV) L1 protein, consisting of: a) expressing a HPV L1 gene encoding the HPV L1 protein in an b) disrupting the c) precipitating the HPV L1 protein from the supernatant of b) by decreasing the salt concentration of the supernatant of b) to from 100 mM to 0, inclusive, by using water or a low salt solution, and collecting the resultant precipitate; d) redissolving the precipitate of c) in a salt solution having a salt concentration of from 150 mM to 25006 mM, adding a reductant to it, and then centrifuging the resultant solution, and isolating the supernatant without using chromatography, wherein the supernatant contains the HPV L1 protein with a purity of at least 50%.