Patent ID: 9535042
Date: 2017-01-03
CPC Classifications: G01N,H01J

Claim:
1. A system for effecting biomolecule separations and monitoring said separations by mass spectrometry, the system comprising: a liquid chromatography module configured to effect at least a partial separation of biomolecule components dissolved in a liquid, the dissolved biomolecule components being carried through the liquid chromatography module in a first fluidic stream; a sampling module in fluid communication with and provided downstream of the liquid chromatography module, the sampling module being configured to periodically transfer an aliquot of the liquid in the first fluidic stream into a second fluidic stream; a dialysis module in fluid communication with and provided downstream of the sampling module, the dialysis module being configured to dialyse the liquid in the second fluidic stream; a mass spectrometer in fluid communication with and provided downstream of the dialysis module, the mass spectrometer being configured to detect components in the dialysed liquid in the second fluidic stream; and, a flow splitting module configured to split the second fluidic stream, the flow splitting module being provided downstream of the sampling module and prior to the dialysis module, the splitting of the second fluidic stream effecting a reduction in the flow of liquid that is passed into and dialysed by the dialysis module; wherein operably, the first fluidic stream is provided at a first flow rate and the second fluidic stream is provided at a second flow rate, independent of the first flow rate such that the dialysis module operates at a constant flow rate.