Patent ID: 9546374
Date: 2017-01-17
CPC Classifications: C12N,Y02A

Claim:
1. An isolated nucleic acid fragment encoding an artificial microRNA (amiRNA) precursor, said fragment comprising a deoxyribonucleotide sequence wherein (i) nucleotides 249 to 269 of SEQ ID NO:16 are replaced by a first variable nucleotide subsequence ranging in size from 19 to 24 nucleotides depending upon a target sequence whose expression is to be reduced, (ii) nucleotides 316 to 336 of SEQ ID NO:16 are replaced by a second variable nucleotide subsequence ranging in size from 19 to 24 nucleotides, said second variable nucleotide subsequence being capable of hybridizing to the first variable subsequence, and (iii) the amiRNA precursor produced by said isolated nucleic acid fragment has the same stem structure as the precursor miRNa produced by endogenous SEQ ID NO:16.