Patent ID: 9675954
Date: 2017-06-13
CPC Classifications: B01J

Claim:
1. A method for producing a microarray, comprising a) providing a first original array comprising a first support surface comprising reference spots that comprise reference molecules, to which at least one template molecule is bound, b) providing a second support surface (receiver surface), which is brought into a spatial orientation relative to the first support surface, c) providing a liquid, containing an enzymatic and/or chemical reaction system, between the first and second support surfaces, so that regions of the two surfaces are in contact with one another via the liquid, d) producing product molecules using the template molecule and the enzymatic and/or chemical reaction system from said reference molecules, e) transferring the product molecules to the second support surface via the liquid between the first and second support surfaces, wherein a correlation exists between a binding site of the template molecule to the first support surface and an accumulation of the corresponding product molecules on the second support surface, and f) detecting the accumulation of the product molecules on the receiver surface in real time, wherein a production of the product molecules according to steps e) and f) is detected in real time, and the producing of d), the transferring of e) and the detecting of f) are terminated as soon as a predefined reference spot size has been reached.