Patent ID: 9655952
Date: 2017-05-23
CPC Classifications: A01N,A61K,A61P,B82Y

Claim:
1. A process for the preparation of a nanoparticle, the nanoparticle having a substantially bi-concaved disc shape, wherein the nanoparticle comprises an aqueous core, a bi-layered shell comprising an amphiphilic polymer, and a payload comprising an oxygen-carrying agent, an allosteric effector, and a reducing agent; the process comprising: (a) hydrophobically modifying a branched polymer by covalently conjugating lipids to at least 25% of the free reactive groups of the branched polymer to form an amphiphilic polymer; (b) mixing the amphiphilic polymer with a non-polar solvent, and agitating the mixture to form a plurality of inverted micelles comprising the amphiphilic polymer; (c) mixing the payload with a polar solvent and adding the payload mixture to the mixture from (b) to form a bi-phasic mixture, and agitating the bi-phasic mixture to transfer the payload to the inverted micelles; and (d) agitating the inverted micelles comprising the payload in the presence of heat and an aqueous solvent to form the bi-concaved disc shaped nanoparticles comprising the payload.