Patent ID: 9670484
Date: 2017-06-06
CPC Classifications: A61K,C07K,C12N,C40B,G01N

Claim:
1. A genetically encoded polypeptide library of complexes comprising: (a) a nucleic acid genetically encoding a polypeptide of the polypeptide library; (b) the genetically encoded polypeptide library displayed on a phage display system, wherein the polypeptide comprises the sequence (X)IC(X)mC(X)nC(X)o (SEQ ID No. 67), wherein C represents the amino acid cysteine, X represents a random amino acid, m and n are numbers between 1 and 20 defining the length of intervening polypeptide segments and I and o are numbers between 0 and 20 defining the length of the flanking polypeptide segments; (c) the polypeptide encoded by the nucleic acid is linked to the nucleic acid by the phage; and (d) the polypeptides in the polypeptide library are attached to tris-(bromomethyl)benzene (TBMB) by three discrete covalent bonds between said TBMB and said polypeptide via said C amino acid residues, and form at least one loop which comprises a sequence of two or more amino acids subtended between two of said C amino acid residues, thus forming the one or more peptide ligand.