Patent ID: 9708582
Date: 2017-07-18
CPC Classifications: C12N

Claim:
1. A method of generating a population of enriched mammalian endodermal progenitor cells comprising: a) maintaining mammalian pluripotent stem cells in a first stem cell medium having an osmolality of 260-310 mOsm/kg; b) priming the mammalian pluripotent stem cells of step a) for endoderm induction by incubating the cells from a) in a second stem cell culture medium having an osmolality of 350 to 550 mOsm/kg for 16 to 60 hours; and c) differentiating the primed mammalian cells from b) in a differentiation medium having an osmolality of 260-310 mOsm/kg to enrich for endodermal progenitor cells; wherein the mammalian endodermal progenitor cells derived from the mammalian pluripotent stem cells express one or more definitive endoderm markers selected from the group consisting of CXCR4, SOX17, GATA-4, FOXA2, AFP, CER1, C-KIT, EPCAM, SNAIL, GSC E-Cad, and N-Cad, and wherein at least 50% of the differentiated mammalian cells produced in c) express the one or more definitive endoderm markers.