Patent ID: 9745622
Date: 2017-08-29
CPC Classifications: C12Q

Claim:
1. A method for detecting a CpG methylation of SDC2 gene for colorectal cancer diagnosis, the method comprising the steps of: (a) isolating genomic DNA from a clinical sample comprising one selected from the group consisting of colorectal tissue, colorectal cells, feces, and blood, obtained from a human subject; (b) treating the genomic DNA or a fragment thereof with bisulfite, wherein the genomic DNA or fragment thereof is treated with bisulfite in such a way that cytosine is converted into a base that is different in its base-pairing behavior in the DNA duplex; and (c) determining hypermethylation of a CpG of SDC2 gene in the genomic DNA or fragment thereof treated with bisulfite according to step (b), wherein colorectal cancer is detected in the human subject based on increased CpG methylation of the SDC2 gene compared with a normal sample, and wherein the hypermethylation of the CpG is determined in a region of the SDC2 gene amplified by a primer pair of SEQ ID NOs: 25 and 26 or a primer pair of SEQ ID NOs: 27 and 28.