Patent ID: 9701710
Date: 2017-07-11
CPC Classifications: C07K,C12N,C12Y

Claim:
1. A method for the purification of a divalent cation binding protein comprising the steps of: (a) loading a first anion exchange resin material with the divalent cation binding protein in a loading buffer in the absence of divalent cations or at a low concentration thereof; (b) eluting the divalent cation binding protein with an eluant comprising a counter-anion to form an eluate containing the divalent cation binding protein; (c) supplementing the eluate obtained in step (b) with at least one divalent cation and increasing the pH; (d) loading a second anion exchange resin material with the supplemented eluate obtained in step (c); and (e) collecting the flow-through from step d) containing the divalent cation binding protein, wherein the divalent cation binding protein is at least 95% w/w pure with respect to host cell protein impurities.