Patent ID: 9625466
Date: 2017-04-18
CPC Classifications: C07K,G01N

Claim:
1. A composition comprising an elongation factor-Ts (EF-Ts) and a stable ternary complex of an elongation factor-Tu (EF-Tu), GTP and an aminoacylated tRNA (aa-tRNA), wherein the EF-Ts is in a binary complex with the EF-Tu and wherein at least 80% of the EF-Tu is labeled with a fluorophore or a fluorescence quencher, and wherein the aa-tRNA is labeled with a fluorophore when the EF-Tu is labeled with a quencher or the aa-tRNA is labeled with a quencher when the EF-Tu is labeled with a fluorophore, wherein the quencher is in sufficient proximity to the fluorophore to form a quenched ternary complex.