Patent ID: 9623048
Date: 2017-04-18
CPC Classifications: A61K,A61P,C12N,G01N

Claim:
1. A method of generating human hepatocyte-like cells, comprising (i) forcing overexpression in a non-hepatic cell of a FOXA polypeptide and a plurality of HNF polypeptides by nucleic acid transfection, virus infection or protein transduction, and (ii) culturing the non-hepatic human cell in a medium for a period of time to obtain one or more progeny cells thereof, thereby generating human hepatocyte-like cells, wherein the HNF polypeptide comprises HNF4A and HNF1, and the FOXA polypeptide comprises FOXA3, wherein the HNF1 polypeptide comprises HNF1A or HNF1B or both, and wherein the non-hepatic human cell does not express a GATA4 or HHEX polypeptide.