Patent ID: 9741110
Date: 2017-08-22
CPC Classifications: C12M,G06K,G06T

Claim:
1. A cell culture evaluation system comprising: a culture device for static culture in a culture vessel; and a measuring device configured to measure suspension cells to be cultured by the culture device, wherein the measuring device includes (i) image acquiring means configured to acquire images of the suspension cells in the culture vessel, (ii) average projected area calculating means configured to extract single-cells from image data acquired by the image acquiring means at a certain culture time, to calculate a projected area of the extracted single-cells, and to calculate an average projected area of the single-cells, and (iii) a lag time determining means configured to determine a lag time by correlating the average projected area of the extracted single-cells with lag times and average projected areas of single cells from a previously-cultured sample, wherein the culture device comprise a culture vessel stage configured to have the culture vessel mounted thereon, wherein the measuring device includes an illumination device positioned to illuminate from above the culture vessel mounted on the culture vessel stage, wherein the image acquiring means of the measuring device is positioned to acquire the images of the suspension cells in the culture vessel from below the culture vessel, such that the culture vessel stage is between the illumination device and the image acquiring means, wherein the measuring device determines a lag time of the cultured suspension cells from the average projected area of the single-cells calculated by the average projected area calculating means, and wherein the measuring device acquires the images, calculates the average projected area, and determines the lag time, when a preset time has elapsed from initiation of the culture in the culture vessel.