Patent ID: 9632026
Date: 2017-04-25
CPC Classifications: G01N

Claim:
1. A method for measuring a bulk refractive index and a biomolecular interaction in a single sensing region of a biosensing system comprising the steps of: injecting a sample comprising an analyte through a flow cell comprising a single sensing region, wherein a first portion of the sensing region has a ligand immobilized thereon and a second portion of the sensing region is free of immobilized ligand; following the injection of the sample, interrogating the entire sensing region using monochromatic light over a single range of increasing reflectance angles thereby generating two or more surface plasmon resonance dips for said single sensing region; measuring a first response at the first portion and a second response at the second portion as the sample flows over the sensing region; and subtracting the second response from the first response to determine a binding response attributed to the interaction of the analyte with the ligand.