Patent ID: 9670518
Date: 2017-06-06
CPC Classifications: C12N,C12P

Claim:
1. A method of producing an isoprenoid comprising: (a) providing a host cell that is genetically modified to comprise an exogenous nucleic acid encoding one or more enzymes of the mevalonate (MEV) pathway chromosomally integrated into the host genome to produce an isoprenoid from the MEV pathway in a greater amount compared to a parent host cell lacking said genetic modification, wherein the one or more enzymes of the MEV pathway are selected from the group consisting of: (b) culturing a single strain of the host cell that is genetically modified in a build stage to produce an inoculum, wherein a population of the host cell that is genetically modified is cultured for a period of time sufficient for said population to undergo a plurality of doublings in an aqueous culture medium comprising a carbon source and a limiting amount of exogenously-provided pantothenate below 0.2 mg/L, wherein the limiting amount of pantothenate limits the production of isoprenoid from the MEV pathway by the population; followed by (c) culturing the population or subpopulation thereof of the host cell that is genetically modified in a production stage for a period of 3 to 20 days, wherein said inoculum is transferred to an aqueous culture medium comprising a carbon source and a non-limiting amount of exogenously-provided pantothenate above 0.2 mg/L, wherein isoprenoid production from the MEV pathway by the population or subpopulation thereof is increased in the presence of the non-limiting amount of pantothenate.