Patent ID: 9650659
Date: 2017-05-16
CPC Classifications: B01L,C12Q,G01N

Claim:
1. A method of screening to identify a single cell organism having a mutation that affects its phototaxis, the method comprising the steps of: (a) obtaining a collection of single cell organisms, wherein one or more of the single cell organisms may have a mutation that affects its phototaxis; (b) pretreating the collection of single cell organisms by culturing each organism under continuous light, wherein the light has a wavelength of 540-600 nm or 430-500 nm and an intensity of 20-50 μmol of photons m (c) irradiating the collection of single cell organisms with light to induce phototaxis; (d) calculating the phototactic indices of each culture of the single cell organisms; and (e) selecting a single cell organism as the desired single cell organism having a mutation in phototactic ability, if the phototactic indices of the selected single cell organism differ from those of a control group of phototactically normal single cell organisms.