Patent ID: 9540440
Date: 2017-01-10
CPC Classifications: A61K,C07K,G01N

Claim:
1. An activatable antibody that in an activated state binds Epidermal Growth Factor Receptor (EGFR) comprising: an antibody or an antigen binding fragment thereof (AB) that specifically binds to EGFR, wherein the AB comprises: (i) a heavy chain amino acid sequence selected from the group consisting of SEQ ID NO: 2, SEQ ID NO: 34, and SEQ ID NO: 36, and (ii) a light chain amino acid sequence comprising the amino acid sequence of SEQ ID NO: 32; wherein the light chain amino acid sequence is coupled to a spacer sequence and a masking moiety (MM) via a cleavable moiety (CM) to produce a masked light chain, wherein the masked light chain comprises an amino acid sequence selected from the group consisting of SEQ ID NO: 4, SEQ ID NO: 6, SEQ ID NO: 8, SEQ ID NO: 10, and SEQ ID NO: 12, wherein the MM inhibits the binding of the AB of the activatable antibody in an uncleaved state to EGFR; wherein the CM is a polypeptide that functions as a substrate for a protease; and wherein the activatable antibody in the uncleaved state has the structural arrangement from N-terminus to C-terminus as follows: Spacer Sequence-MM-Linking Peptide 1 (LP1)-CM-Linking Peptide 2 (LP2)-AB or AB-LP2-CM-LP1-MM-Spacer Sequence.