Patent ID: 9714940
Date: 2017-07-25
CPC Classifications: B82Y,C12Q,G01N,G01R

Claim:
1. A method of detecting a pathogen in a whole blood sample, said method comprising the following steps: (a) contacting a whole blood sample obtained from a subject with an erythrocyte lysis agent, thereby lysing red blood cells; (b) centrifuging the product of step (a) to form a supernatant and a pellet; (c) discarding some or all of the supernatant of step (b) and resuspending the pellet to form an extract, optionally washing the pellet prior to resuspending the pellet, and optionally repeating the centrifuging, discarding, and resuspending steps of (b) and (c); (d) combining the extract of step (c) with beads to form a mixture and agitating the mixture to form a lysate, said lysate containing both subject cell nucleic acid and pathogen nucleic acid; (e) amplifying a target nucleic acid in said lysate of step (d) specific to said pathogen by polymerase chain reaction (PCR) to form an amplified lysate solution; (f) following step (e), adding to the amplified lysate solution from 1×10 (g) exposing the detection mixture to a bias magnetic field and an RF pulse sequence, thereby producing a signal; (h) following step (g), measuring the signal from the detection mixture; and (i) on the basis of the result of step (h), detecting the target nucleic acid, thereby determining whether the pathogen was present in the sample.