Patent ID: 9708650
Date: 2017-07-18
CPC Classifications: C12Q

Claim:
1. A method for sequencing DNA, comprising the steps of: a. amplifying DNA in a first reaction mixture comprising nuclease-sensitive amplification primers to form amplified DNA; b. contacting the first reaction mixture of the amplifying step with a second reaction mixture comprising a nuclease and a chemically-enhanced primer, the chemically-enhanced primer comprises an oligonucleotide sequence having a nuclease resistance inter-nucleotide linkage and a negatively charged moiety (NCM), and the NCM comprises a moiety having a structure of the following formula: wherein each n is independently an integer of 1 to 9, and x is an integer of 2 to about 30; c. inactivating the nuclease; and d. reacting the amplified DNA in a sequencing reaction wherein the chemically-enhanced primer primes the sequencing reaction.