Patent ID: 9657352
Date: 2017-05-23
CPC Classifications: C12Q

Claim:
1. A combination of at least two oligomers for detecting in a sample a Megasphaera sp. 16S rRNA or a gene encoding a Megasphaera sp. 16S rRNA, said oligomer combination comprising: a first amplification oligomer comprising a first target-hybridizing sequence consisting of 14 to 32 contiguous nucleotides contained in the sequence of SEQ ID NO: 38 and that includes at least the sequence of SEQ ID NO: 37; and a second amplification oligomer that is a promoter primer comprising (i) a second target-hybridizing sequence consisting of 22 to 31 contiguous nucleotides contained in the sequence of SEQ ID NO: 40 and that includes at least the sequence of SEQ ID NO: 21 and (ii) a T7 promoter sequence located 5′ to the second target-hybridizing sequence.