Patent ID: 9746484
Date: 2017-08-29
CPC Classifications: A61K,C07C,C07K,G01N

Claim:
1. A method of determining the amount of 25-OH vitamin D analyte in a sample suspected of containing the 25-OH vitamin D analyte, the method comprising: (a) providing in combination in an assay medium: (b) incubating the assay medium under conditions for binding of the vitamin D epimer binding partner to the epimeric vitamin D and for binding of the 25-OH vitamin D binding partner to the 25-OH vitamin D analyte to form a vitamin D binding partner-bound complex; and (c) determining the amount of the vitamin D binding partner-bound complex and relating the amount of the vitamin D binding partner-bound complex to the amount of the 25-OH vitamin D analyte in the sample; wherein said vitamin D binding partner is an antibody that is specific for 25-OH vitamin D analyte but having cross-reactivity to the epimeric vitamin D; said epimeric vitamin D is 3-epi-25-hydroxyvitamin D and wherein said vitamin D epimer binding partner is an antibody that specifically binds said 3-epi-25-hydroxyvitamin D but does not bind to any detectable degree with 25-OH vitamin D.