Patent ID: 9637720
Date: 2017-05-02
CPC Classifications: A01N,C05F,C12N,Y02P,Y02W

Claim:
1. A method for multiplying a strain of Trichoderma , which comprises: inoculating, with a stock suspension of culturing the culture medium thus inoculated so as to obtain a primary inoculum in which the multiplication factor of the propagules reaches 2×10 wherein said fibers are obtained by extrusion of wood shavings in a twin-screw extruder by a method comprising: introducing wood shavings into a defiberizing barrel containing two parallel screws driven rotationally so as to engage with one another via their respective threads, said threads having successively, in the direction from upstream to downstream, at least one upstream series and one downstream series of sections each comprising an upstream drive zone in which the shavings are driven in the downstream direction, and a downstream slowing down zone through which the shavings are forced under the effect of drive provided by the upstream zone, the shavings being driven in the barrel while being slowed down in the slowing down zones and being converted into fibers that can be recovered at the outlet of the defiberizing barrel, the residence time of the shavings in the barrel being between 15 and 80 seconds, a pressure at least about 90 bar being provided upstream of the downstream slowing down zone of the upstream series, such that a temperature of between 120° C. and 150° C. is reached in the barrel without external provision of heat to the defiberizing barrel.