Patent ID: 9651765
Date: 2017-05-16
CPC Classifications: G01N,G02B

Claim:
1. A method for separating detection signals in a beam path of a fluorescent microscope, including a photon-counting detector for detecting an optical signal including fluorescent light, wherein the detection signals are signals resulting from fluorescent light and from reflected light or scattered light that strikes the photon-counting detector in the beam path, different of the detection signals being formed in a defined temporal sequence, the method comprising: providing pulsed excitation light to excite the fluorescent light from a sample; performing a suppression or separation of the detection signals on the basis of the temporal sequence; integrating or summing electrical signals resulting from photons impinging on the photon-counting detector in a downstream integrator and being assigned to pixels; filtering out reflection signals between the photon-counting detector and the integrator via a gate, triggered by a gating signal, the gate being synchronized with the pulsed excitation light, wherein the triggering by the gating signal is performed on an edge of a photon-counting signal of the photon-counting detector detecting the optical signal including fluorescent light.