Patent ID: 9638699
Date: 2017-05-02
CPC Classifications: G01N

Claim:
1. A method of detecting glutathionylation of β1 subunit of human erythrocyte ATP-dependent Na+K+ pump protein in an individual, said method comprising a) obtaining a blood sample from the individual, b) contacting erythrocyte membranes prepared from said sample with a first antibody specific for glutathione bound to a cysteine residue at position 45 within SEQ ID NO: 1 of the β1 subunit of the human erythrocyte ATP-dependent Na+K+ pump protein and a second antibody specific for the β1 subunit of the human erythrocyte ATP-dependent Na+K+ pump protein comprising the cysteine residue at position 45 within SEQ ID NO: 1, and c) detecting the binding between the first antibody and glutathione bound to a cysteine residue at position 45 within SEQ ID NO: 1 of the β1 subunit of the human erythrocyte ATP-dependent Na+K+ pump protein in the blood sample from the individual and the binding between the second antibody and the β1 subunit of the human erythrocyte ATP-dependent Na+K+ pump protein comprising the cysteine residue at position 45 within SEQ ID NO: 1 in the blood sample from the individual.