Patent ID: 9541620
Date: 2017-01-10
CPC Classifications: G01N,G01R,G16B

Claim:
1. A method of determining measures of trimethylamine-N-oxide (TMAO) in an in vitro biosamples, comprising: generating, using a nuclear magnetic resonance (NMR) spectrophotometer, an NMR spectrum for a biosample; defining, using one or more processors, a TMAO peak region in which a TMAO peak is expected to be located, the TMAO peak region being defined to between about 3.2 and 3.4 ppm; identifying, using the one or more processors, a reference location of a reference peak that is within a reference peak region in the NMR spectrum, the reference peak region being defined based on a reference NMR spectrum generated using a reference sample; detecting, using the one or more processors, a calibration location corresponding to a pH sensitive calibration peak within a calibration peak region in the NMR spectrum; identifying, using the one or more processors, a TMAO peak location that is a location of a peak that is within the TMAO peak region in the NMR spectrum and that corresponds to a concentration of TMAO in the biosample, the identification being based on: detecting an amplitude of the NMR spectrum at the TMAO peak location; calculating, using the one or more processors, a TMAO concentration metric based on the amplitude of the NMR spectrum at the TMAO peak location; and generating an electronic report that includes the TMAO concentration metric, wherein the measure of TMAO is substantially linear and detects TMAO in a range of from 1-50 μM TMAO.