Patent ID: 9587214
Date: 2017-03-07
CPC Classifications: C12M,Y10T

Claim:
1. A method of ventilating a bioreactor comprising: a. providing a bioreactor capable of holding cell culture and culture medium and growing the cell culture: b. providing a bioreactor exhaust assembly comprising: c. attaching the lower end of the bioreactor exhaust assembly to a bioreactor; d. operating the bioreactor to grow a cell culture; e. cooling the vertical surface to maintain a temperature in the exhaust tube below the dew point of a gas in the exhaust tube; f. allowing the exhaust from the bioreactor to enter the exhaust tube; g. measuring the pressure in the exhaust tube using the pressure sensor; h. releasing the exhaust gas to atmosphere by opening the low pressure relief valve when the pressure in the exhaust assembly reaches a pressure above 0.05-inch (±10%) water gauge; i. closing the low pressure relief valve and stopping the exhaust of gas to atmosphere when the pressure inside the exhaust assembly reaches below 0.05-inch (±10%) water gauge; and j. repeating steps (f)-(h) till the end of the bioreaction cycle.