Patent ID: 9689033
Date: 2017-06-27
CPC Classifications: C07K,C12Q,G01N

Claim:
1. A method of characterizing a target polynucleotide, the method comprising: (a) applying a potential difference across a pore in contact with a Hel308 helicase and the target polynucleotide; (b) measuring at least two electrical signals for a unit of one or more nucleotides of the target polynucleotide moving through the pore during a full translocation cycle of the Hel308 helicase; (c) characterizing the target polynucleotide using the at least two electrical signals measured in (b); and wherein the unit of one or more nucleotides of the target polynucleotide passing through the pore during a full translocation cycle of the Hel308 helicase is characterized with an accuracy greater than 50% using the at least electrical two signals measured in (b), compared to characterization of the unit of one or more nucleotides of the target polynucleotide using a single electrical signal measured during the full translocation cycle.