Patent ID: 9695076
Date: 2017-07-04
CPC Classifications: C02F

Claim:
1. A method for reducing release of resistance genes during sludge anaerobic treatment, with the method carried out by using a system including a concentration tank, a supersonic pre-treatment device, an anaerobic treatment device, a real-time fluorescent quantitative polymerase chain reaction (PCR) instrument, a computer, an alkali storage tank, an electric valve, and a pH detector, with the concentration tank including a bottom connected by a conduit and a valve to a sludge inlet in an upper portion of the supersonic pre-treatment device, with the supersonic pre-treatment device including a sludge outlet connected to the anaerobic treatment device, with the anaerobic treatment device including a lower portion having an outlet for residual sludge, with the anaerobic treatment device further including an upper portion having an outlet for a supernatant liquid, with the residual sludge and the supernatant liquid being detected while passing through the real-time fluorescent quantitative PCR instrument, with the alkali storage tank including a bottom connected by a conduit and the electric valve to an inlet in a top of the anaerobic treatment device, with the pH detector mounted on the upper portion of the anaerobic treatment device, with the computer connected to the real-time fluorescent quantitative PCR instrument, the electric valve, and the pH detector, with the method comprising: (1) controlling concentration of to-be-treated sludge in the concentration tank to be 12-20 g/L by sedimentation under gravity; (2) transferring the concentrated sludge into the supersonic pre-treatment device to proceed with supersonic pre-treatment, with the supersonic pre-treatment conducted for 5-30 minutes at a power of 0.1-0.5 kW and a frequency of 10-40 kHz; (3) transferring the pre-treated sludge into the anaerobic treatment device for anaerobic treatment, with the anaerobic treatment conducted for 4-12 days at a temperature of 20-37° C.; (4) detecting a release amount of resistance genes in residual sludge and the supernatant liquid in the anaerobic treatment device with the real-time fluorescent quantitative polymerase chain reaction instrument; and (5) controlling a feedback dosage of an alkali liquid according to the release amount of the resistance genes and feeding the feedback dosage of the alkali liquid into the anaerobic treatment device to control a pH value to be 9.0-11.0 during the anaerobic treatment.