Patent ID: 9624522
Date: 2017-04-18
CPC Classifications: C12Q,G01N

Claim:
1. A single, direct measurement method for measuring the fraction of a protein that is a glycated protein in a test sample comprising said protein and said glycated protein, said method comprising: (a) contacting a first binding ligand with said test sample under conditions wherein said first binding ligand binds selectively and equivalently to said protein and said glycated protein in direct proportion to the fraction of said glycated protein in said test sample to form a first complex, wherein said first binding ligand is attached to a second solid support; (b) contacting said sample containing first complex, with a second binding ligand under conditions wherein said first complex and said second binding ligand bind to form a second complex, wherein said second binding ligand comprising a peroxide-generating system and wherein said second binding ligand binds specifically to said glycated protein of said first complex; (c) isolating said second complex; (d) contacting said second complex with a substrate for said peroxide-generating system under conditions wherein a peroxide is generated to form an assay mixture; (e) contacting the assay mixture with a first solid support comprising an electrode comprising a covalently attached electroactive moiety (EAM) having a first E (f) measuring the electrochemical properties of said EAM at the first E (g) determining the fraction of said glycated protein from said electrochemical properties.