Patent ID: 9593379
Date: 2017-03-14
CPC Classifications: C12Q

Claim:
1. A method of diagnosing breast cancer, the method comprising: providing a biological sample comprising a vesicle obtained from a subject; incubating the biological sample with an antibody or antigen binding fragment thereof that specifically binds to a surface protein of the vesicle, wherein the surface protein is CD83, CD9, EpCAM, caveolin, FasL, HLA-DRA, CD36, CD63, CD81, MUC1, ERBB4, GPER, ERBB2, MILANA, AMHR2, or a combination thereof; isolating the antibody or antigen binding fragment thereof bound vesicle from the biological sample; measuring an amount of microRNA (miRNA) in the isolated vesicle, the microRNA being at least one selected from the group consisting of hsa-miR-126, hsa-miR-23a, hsa-miR-24, hsa-miR-19b, hsa-miR-103, hsa-miR-142-3p, hsa-miR-144, hsa-miR-15a, hsa-miR-185, and hsa-miR-93; comparing the measured amount of the microRNA with a control; and providing a breast cancer diagnosis based upon the comparison of the amount of the microRNA from the sample with a control, wherein providing a breast cancer diagnosis comprises determining if the measured amount of the microRNA from the biological sample is greater or lesser than the measured amount of the microRNA from the control, wherein when the control is an amount of microRNA from a biological sample from a subject having no breast tumor or a benign breast tumor, an increased amount of microRNA from the biological sample relative to the control is indicative of breast cancer in the subject, and wherein when the control is an amount of microRNA from a biological sample from a subject having breast cancer, a decreased amount of microRNA from the biological sample relative to a control is indicative of non-breast cancer diagnosis.