Patent ID: 9658224
Date: 2017-05-23
CPC Classifications: G01N

Claim:
1. A method of generating an antibody-displaying bacteriophage that does not contain a nucleic acid sequence encoding the displayed antibody, the method comprising the steps of: a) administering a non-phage vector comprising a nucleic acid that lacks a phage packaging sequence to a bacterial cell, wherein the nucleic acid encodes a fusion protein comprising an antibody and a polypeptide that will be displayed on the outer surface of a bacteriophage, and wherein the bacterial cell produces the fusion protein; b) infecting the bacterial cell with a phage, wherein the phage comprises a detectable marker molecule, wherein phage particles are produced in the bacterial cell; and c) assembling a bacteriophage in the bacterial cell, wherein the bacteriophage comprises the detectable marker molecule and displays the fusion protein on its outer surface and does not contain the nucleic acid sequence encoding the displayed antibody.