Patent ID: 9714412
Date: 2017-07-25
CPC Classifications: A01N,A61P,C12N

Claim:
1. A method for freezing a pluripotent stem cell, the method including the steps of providing a pluripotent stem cell suspension, performing ice nucleation on the pluripotent stem cell suspension by rapidly lowering the temperature of the suspension to a nucleating point at a rate from about −5° C./minute to about −55° C./minute to provide an ice nucleated pluripotent stem cell suspension, and lowering the temperature of the ice nucleated pluripotent stem cell suspension to a temperature sufficiently low to allow long term storage of the pluripotent stem cell, wherein upon thawing, the pluripotent stem cell retains pluripotency, and wherein the pluripotent stem cell is a human embryonic stem cell.