Patent ID: 9650604
Date: 2017-05-16
CPC Classifications: A61K,C12N

Claim:
1. A method for producing equine amniotic membrane-derived multipotent stem cells comprising the steps of: (1) isolating cells from an equine amniotic membrane; (2) culturing the isolated cells in low glucose Dulbecco's modified Eagle medium (LG-DMEM); and (3) harvesting the cultured cells, wherein the equine amniotic membrane-derived multipotent stem cells cultured by the step (2) are selected for; (a) showing negative immunological responses to all of human markers CD19, CD20, CD28, CD31, CD34, CD38, CD41a, CD62L, CD62P and CD200, and positive immunological responses to all of human markers CD44, CD90 and CD105; and (b) having the ability to be maintained in an undifferentiated state for 14 passages or more.