Patent ID: 9639660
Date: 2017-05-02
CPC Classifications: C12Q,G16B

Claim:
1. A method for quantifying an expression level of a target gene, comprising: 1) synthesizing cDNA from RNA of a subject; 2) performing real-time PCR to amplify the target gene using a pair of primers and/or probes with the cDNA serving as a template and then performing real-time PCR to amplify an endogenous reference gene, wherein said endogenous reference gene is selected by a method comprising (a) computing expression levels of genes from EST (expressed sequence tag), SAGE (serial analysis of gene expression), and microarray datasets; and (b) identifying constitutively expressed genes from more than one tissue type based on the computed gene expression levels of step (a) and zero(0)'s proportions thereof; and 3) normalizing an expression level of the target gene to that of the endogenous reference gene of step 2).