Patent ID: 9597383
Date: 2017-03-21
CPC Classifications: A61K,C12N

Claim:
1. A method for preparing antigen specific T cells comprising the following steps: (a) obtaining human peripheral blood mononuclear cells; (b) culturing the cells with IL-15 and 0.5-20 ng/mL IL-12; (c) sorting dendritic killer cells, wherein the dendritic killer cells are cells with the cell surface marker HLA-G (d) co-culturing the dendritic killer cells with a plurality of target cells, wherein the target cells express a specific antigen; (e) harvesting the dendritic killer cells presenting the specific antigen; (f) mixing the dendritic killer cells presenting the specific antigen with a cell population of T cells and co-culturing for a period; and (g) harvesting the antigen specific T cells.