Patent ID: 9670485
Date: 2017-06-06
CPC Classifications: C12N

Claim:
1. A method for isolating microbial DNA from a sample that comprises host DNA and microbial DNA, comprising: a) obtaining a tagged DNA sample tagged with a universal adaptor, wherein the tagged DNA sample contains host DNA and microbial DNA which both comprise the universal adaptor; b) hybridizing the tagged DNA, in solution, with affinity-tagged RNA probes, wherein the affinity-tagged RNA probes comprise sequences complementary to sequences of the host DNA, and wherein the affinity-tagged RNA probes comprise affinity-tagged ribonucleotides; c) binding the product of step b) with a capture agent that is tethered to a substrate, in the presence of RNA oligonucleotides that are complementary to or have the same sequence as one or more strands of the universal adaptor, thereby capturing the host DNA on the substrate; and d) collecting the unbound DNA, wherein the unbound DNA comprises the microbial DNA.