Patent ID: 9725774
Date: 2017-08-08
CPC Classifications: C12Q

Claim:
1. A method for detecting dengue virus in a biological sample, the method comprising: (a) contacting a biological sample suspected of containing dengue virus with a set of primers comprising: (i) a forward primer comprising the sequence of SEQ ID NO:5 and a reverse primer comprising the sequence of SEQ ID NO:9; (ii) a forward primer comprising the sequence of SEQ ID NO:5 and a reverse primer comprising the sequence of SEQ ID NO:10; (iii) a forward primer comprising the sequence of SEQ ID NO:5 and a reverse primer comprising the sequence of SEQ ID NO:11; (iv) a forward primer comprising the sequence of SEQ ID NO:6 and a reverse primer comprising the sequence of SEQ ID NO:10; (v) a forward primer comprising the sequence of SEQ ID NO:6 and a reverse primer comprising the sequence of SEQ ID NO:11; (vi) a forward primer comprising the sequence of SEQ ID NO:7 and a reverse primer comprising the sequence of SEQ ID NO:9; (vii) a forward primer comprising the sequence of SEQ ID NO:8 and a reverse primer comprising the sequence of SEQ ID NO:12; (viii) a forward primer and a reverse primer each comprising at least 10 contiguous nucleotides from the corresponding nucleotide sequences of the forward primer and reverse primer of a primer set selected from the group consisting essentially of (i)-(vii); (ix) a forward primer and a reverse primer comprising at least one nucleotide sequence that differs from the corresponding nucleotide sequence of the forward primer or reverse primer of a primer set selected from the group consisting essentially of (i)-(vii) in that the primer has up to three nucleotide changes compared to the corresponding sequence, wherein the primer is capable of hybridizing to and amplifying dengue virus nucleic acids in the nucleic acid amplification assay; (x) a forward primer and a reverse primer comprising nucleotide sequences that are complements of the corresponding nucleotide sequences of the forward primer and reverse primer of a primer set selected from the group consisting essentially of (i)-(ix); or (xi) a combination of a primer set selected from the group comprising (i)-(x), (b) amplifying dengue virus nucleic acids if present, said nucleic acids comprising at least one target sequence selected from the group consisting essentially of a dengue-1 target sequence, a dengue-2 target sequence, a dengue-3 target sequence, and a dengue-4 target sequence; (c) detecting the presence of the amplified nucleic acids using at least one detectably labeled oligonucleotide probe sufficiently complementary to and capable of hybridizing with the dengue virus RNA or amplicon thereof, if present, as an indication of the presence or absence of dengue virus in the sample.