Patent ID: 9632076
Date: 2017-04-25
CPC Classifications: C12M,G01N

Claim:
1. A method of modeling an ischemic condition, comprising: providing a microfluidic device including a fluid-filled chamber, a channel, a porous barrier separating the chamber from the channel, a first population of CNS cells proximal to the channel, and a second population of CNS cells distal from the channel relative to the first population of CNS cells; concurrently with exposing the first population of CNS cells to a low concentration of oxygen, exposing the second population of CNS cells to a high concentration of oxygen, thereby modeling the ischemic condition, wherein: exposing the first population of CNS cells to a low concentration of oxygen comprises flowing an oxygen scavenger through the channel; and measuring at least one cellular property of the first population of CNS cells.