Patent ID: 9657343
Date: 2017-05-23
CPC Classifications: C12Q

Claim:
1. A method of determining the sequence of a target nucleic acid molecule having an unknown sequence, comprising: (a) combining an RNA polymerase, a target nucleic acid molecule, a solid substrate, and a rotation tag, to form a combination for sequencing in which the target nucleic acid molecule is bound to the rotation tag and the RNA polymerase is immobilized on the solid substrate and complexed with the target nucleic acid molecule; (b) exposing the combination produced in step (a) to sequencing conditions, wherein the sequencing conditions comprise contacting the combination of step (a) with at least one nucleoside triphosphate; (c) detecting the rotational pattern of the rotation tag; (d) repeating the contacting and detecting steps a plurality of times; and (e) determining the sequence of the target nucleic acid molecule based, sequentially, on the presence or absence of a change in the rotational pattern as a result of each exposure to the sequencing conditions.