Patent ID: 9574168
Date: 2017-02-21
CPC Classifications: C12M,C12N

Claim:
1. A method for collecting cultured cells by using a cell culture kit comprising a culture container for cultivating cells, a cell collection container for collecting cells after cultivation and a waste liquid container for collecting a culture medium after cultivation, wherein the culture container, the cell collection container, and the waste liquid container are linked to one another through a conduit, the method comprising: discharging a culture supernatant by transferring the culture supernatant from the culture container to the waste liquid container to obtain a concentrated suspension; collecting cells by transferring the concentrated suspension of the cultured cells from the culture container to the cell collection container; returning part of the culture supernatant that had been discharged into the waste liquid container to the culture container to allow cells remaining in the culture container to be suspended in a suspension of cells; and re-collecting the cells remaining in the culture container by transferring the suspension of the cells from the culture container to the cell collection container.