Patent ID: 9574233
Date: 2017-02-21
CPC Classifications: C12Q

Claim:
1. A method for detecting the synthesis and/or amplification of a target nucleic acid sequence, comprising the steps of: (i) providing at least one set of primers designed for synthesising and/or amplifying at least one region of said target nucleic acid sequence; (ii) combining the set of primers as per step (i) with a reaction mixture for the amplification of said at least one region of the target nucleic acid sequence, said reaction mixture comprising at least one colorimetric metal indicator and at least one magnesium chelator; (iii) placing the reaction mixture obtained from step (ii) in contact with a biological specimen so as to amplify said at least one region of the target nucleic acid sequence possibly present in said specimen; and (iv) verifying a possible change in the colour of the reaction mixture, wherein said colorimetric metal indicator and said magnesium chelator are hydroxynaphthol blue and sodium citrate, respectively.