Patent ID: 9708655
Date: 2017-07-18
CPC Classifications: C12Q,G01N

Claim:
1. A method including: providing a nanopore including a first side, a second side, and an aperture extending through the first and second sides; providing a permanent tether including a head region, a tail region, and an elongated body disposed therebetween, moving the reporter within the aperture toward the first side of the nanopore responsive to a first event occurring adjacent to the first side of the nanopore, wherein the first event comprises addition of a first nucleotide to a polynucleotide; moving the reporter toward the second side of the nanopore after the first event and before a second event; and moving the reporter within the aperture responsive to the second event occurring adjacent to the first side of the nanopore, wherein the second event comprises addition of a second nucleotide to the polynucleotide.