Patent ID: 9683261
Date: 2017-06-20
CPC Classifications: C12P,C12Q,G16B

Claim:
1. A standardized mixture for assessing amounts of at least one target nucleic acid in a sample, comprising: a first competitive template for a first target nucleic acid in a sample, the first competitive template comprising a first competitive template nucleic acid at a first concentration; and, a second competitive template for a second target nucleic acid in the sample, the second competitive template comprising a second competitive template nucleic acid at a second concentration that is different from the first concentration; the first and second competitive templates being at known different concentrations relative to each other; the competitive templates for each target nucleic acid comprising a competitive template nucleic acid that can be amplified using identical primers, shares sequence homology, and amplified with substantially similar efficiency as the target nucleic acid while having one or more features that allow the competitive template to be distinguished from the target nucleic acid selected from the group consisting of: one or more point mutations; one or more insert mutations, one or more inversion mutations; and one or more deletion mutations.