Patent ID: 9611310
Date: 2017-04-04
CPC Classifications: C07K,C12N,C12P

Claim:
1. A method for decreasing nonprocessed Factor VIII (FVIII) in a culturing medium, comprising contacting a nonprocessed FVIII polypeptide expressed from a polynucleotide in a host cell with proprotein convertase subtilisin/kexin type 5 (PC5), wherein the PC5 is expressed from a second polynucleotide in the host cell expressing the FVIII, wherein the host cell is a mammalian cell, and wherein the PC5 processes the nonprocessed FVIII polypeptide to a processed FVIII polypeptide, which comprises a FVIII heavy chain and a FVIII light chain associated by a non-covalent bond.