Patent ID: 9701945
Date: 2017-07-11
CPC Classifications: A61K,A61P,C12N

Claim:
1. A process of production of a virus which comprises replicating a virus selected from the group consisting of adenoviruses, hepadnaviruses, herpes viruses, orthomyxoviruses, papovaviruses, paramyxoviruses, picornaviruses, poxviruses, reoviruses and retroviruses, in cells of an avian cell line wherein said cell line has been obtained by a method comprising the following steps: a. culturing avian embryonic stem cells in a cell culture medium containing the factors allowing their growth, wherein said factors comprise: b. passaging by modifying the culture medium so as to obtain the withdrawal of said exogenous growth factors and cytokines, of the serum, and of the feeder layer; c. establishing adherent or non-adherent cell lines capable of proliferating in the absence of said exogenous growth factors, serum, and inactivated feeder layer from said cell culture medium; said process comprising the steps of: wherein said process comprises an additional step of feeding the cells wherein said feeding step consists in a periodic addition of concentrated solution of glutamine and D-glucose to the culture medium; and wherein glutamine concentration in the culture medium is maintained between 1 mM to 3 mM and D-glucose concentration in the medium is maintained around 1 g/l to 10 g/l of D-glucose.