Patent ID: 9580492
Date: 2017-02-28
CPC Classifications: A61K,A61P,C07K,G01N

Claim:
1. A substantially pure polypeptide that binds a conformational epitope of anthrax edema factor (EF) antigen to which anti-anthrax EF13D monoclonal antibody binds, wherein said monoclonal antibody comprises a heavy chain CDR1 region having an amino acid sequence of SEQ ID NO:35, a heavy chain CDR2 region having an amino acid sequence of SEQ ID NO:37, a heavy chain CDR3 region having an amino acid sequence of SEQ ID NO:39, a light chain CDR1 region having an amino acid sequence of SEQ ID NO:43, a light chain CDR2 region having an amino acid sequence of SEQ ID NO:45, and a light chain CDR3 region having an amino acid sequence of SEQ ID NO:47, and wherein said substantially pure polypeptide is a variant of the anti-anthrax EF13D monoclonal antibody and said substantially pure polypeptide comprises: (a) a heavy chain amino acid sequence that is at least 95% identical to SEQ ID NO: 33 or a light chain amino acid sequence that is at least 95% identical to SEQ ID NO: 41; or (b) a conservative variation in the amino acid sequence of SEQ ID NO: 35, SEQ ID NO:37, SEQ ID NO:39, SEQ ID NO: 43, SEQ ID NO:45 or SEQ ID NO: 47.