Patent ID: 9555127
Date: 2017-01-31
CPC Classifications: A61K,C07H,C12N

Claim:
1. A biological system for diminishing cell growth or inducing selective killing of target cells comprising a vehicle with a first nucleic acid sequence or amino acid sequence encoding for a prokaryotic toxin of a prokaryotic toxin-antitoxin pair, and a second nucleic acid sequence or amino acid sequence encoding for the corresponding prokaryotic antitoxin of the prokaryotic toxin-antitoxin pair, characterized in that the toxin and/or the antitoxin is operably linked to a protein output modifier (POM) that comprises a nucleic acid sequence or amino acid sequence that modifies the relative rate of transcription, mRNA stability, mRNA translatability or protein stability of the toxin and/or antitoxin thereby changing the relative ratio in the concentration of the toxin and/or the antitoxin within the target cells and/or, where applicable, within non-target cells by decreasing the antitoxin outputs in the target cells relative to the toxin outputs, and/or decreasing the toxin outputs in the non-target cells relative to the antitoxin outputs.