Patent ID: 9650442
Date: 2017-05-16
CPC Classifications: A61P,C07K

Claim:
1. A bispecific antibody that binds to human EGFR and human IGF-1R comprising a first antigen-binding site that binds to human EGFR and a second antigen-binding site that binds to human IGF-1R, i) the first antigen-binding site comprises in the heavy chain variable domain a CDR1 of SEQ ID NO:1, a CDR2 of SEQ ID NO:2, and a CDR3 of SEQ ID NO:3, and in the light chain variable domain a CDR1 of SEQ ID NO:4, a CDR2 of SEQ ID NO:5, and a CDR3 of SEQ ID NO:6; and ii) the second antigen-binding site is a modified antigen-binding site based on the heavy chain variable domain comprising a CDR1 of SEQ ID NO:9, a CDR2 of SEQ ID NO:10, and a CDR3 of SEQ ID NO:11; and on the light chain variable domain comprising a CDR1 of SEQ ID NO: 12, a CDR2 of SEQ ID NO:13, and a CDR3 of SEQ ID NO:14; wherein the modified second antigen-binding site comprises one more modifications in one or more of the CDRs; and wherein the modified second antigen binding site has an at least 10-fold increased KD value of the binding affinity for binding to human IGF-1R compared to the unmodified second antigen binding site.