Patent ID: 9617589
Date: 2017-04-11
CPC Classifications: B01J,B01L,C12Q,G01N

Claim:
1. A microfluidic device for delivering reagent primers to a reaction well using a solid support wherein the reagent primers are connected to the solid support via a functional group that is configured to participate in labile bonds, the device comprising: a plurality of reaction wells; and a plurality of solid supports having the reagent primers attached thereto, wherein the plurality of wells and the plurality of solid supports are sized and configured such that only a single one of the plurality of solid supports is retained in a corresponding single one of the plurality of wells, and the microfluidic device comprises a seal which fluidically isolates each of the plurality of wells from each other; wherein the plurality of reaction wells comprises a reaction solution comprising a sample and, in solution, a detectable label to detect nucleic acid amplicons, and reagent primer cleaved from a respective one of the plurality of solid supports.