Patent ID: 9701999
Date: 2017-07-11
CPC Classifications: C12Q

Claim:
1. A method, comprising: a) treating a specific target nucleic acid with a methylation-specific detection reagent, wherein the specific target nucleic acid is a single gene or untranslated region of a single gene; b) simultaneously amplifying a plurality of different nucleic acid segments within said specific target nucleic acid to generate a plurality of different amplicons wherein said simultaneous amplifying is carried out in a single reaction vessel; and c) detecting two or more amplicons of said plurality of different amplicons comprising said different nucleic acids segments within said specific target nucleic acid wherein said detecting comprises contacting said plurality of different amplicons comprising said different nucleic acids segments within said specific target nucleic acid with a plurality of different nucleic acid probes wherein said plurality of different nucleic acid probes comprise a detectable label that is the same label in each of said plurality of different nucleic acid probes.