Patent ID: 9540635
Date: 2017-01-10
CPC Classifications: C07H,C12N

Claim:
1. A process for extracting DNA from cells without cell walls, said process comprising: (a) immersing said cells in an aqueous solution comprising a C1-C4 alcohol at from about 50% to about 85% by weight, a cell lysis detergent at from about 0.05% to about 0.5% by weight, and a buffering agent maintaining a pH of from about 7.5 to about 9.0, and substantially no protease, substantially no reducing agent, and substantially no non-detergent, inorganic salt, at a temperature of from about 15° C. to about 35° C. for from about five seconds to about thirty minutes, to cause DNA to be liberated from said cells and to precipitate; and (b) recovering said DNA so precipitated from said aqueous solution.