Patent ID: 9624530
Date: 2017-04-18
CPC Classifications: C12Q

Claim:
1. A method for preserving genomic DNA complexity during methylation analysis of genomic DNA of a subject, comprising: a) isolating genomic DNA, or a fragment thereof, from a biological sample obtained from the subject; b) contacting the isolated genomic DNA or fragment thereof with at least one enzyme that adds a methyl group to a cytosine nucleotide outside of CpG dinucleotide sequences of the genomic DNA and does not add a methyl group to a cytosine nucleotide within the CpG dinucleotide sequences of the genomic DNA, wherein the at least one enzyme has a recognition sequence that consists of a CpT or CpA dinucleotide and methylates the cytosine of the CpT or CpA dinucleotide it recognizes; c) treating the enzyme-treated genomic DNA, or fragment thereof, of step b) with at least one reagent to convert unmethylated cytosine bases to uracil or to another base that is detectably dissimilar to cytosine in terms of hybridization properties, thereby preserving the DNA complexity; and d) detecting DNA methylation of the preserved and treated genomic DNA, or fragment thereof, of step c), on a genome wide scale.