Patent ID: 9605280
Date: 2017-03-28
CPC Classifications: C12N,C12P,Y02E

Claim:
1. A recombinant E. coli , wherein said E. coli contains a mutated lpdA gene encoding lipoamide dehydrogenase, wherein the polypeptide encoded by the mutated lpdA gene consists of the amino acid sequence SEQ ID No.: I with a modification at position A358 of SEQ ID No.: 1 and optionally modification(s) at one or both positions T81 and P275, SEQ ID No.: 1, wherein the modification at the position T81, is the replacement of T with I; the modification at the position P275 is the replacement of P with S; and the modification at the position A358 is the replacement of A with V; optionally, the expression of the mutated lpdA gene, or the activity of the protein encoded by said mutated lpdA gene, in the recombinant