Patent ID: 9719102
Date: 2017-08-01
CPC Classifications: C12N

Claim:
1. A method of reducing the level of mRNA of at least two sequences from the delta 12 fatty acid desaturase 2 (FAD2) protein and/or gene family in a cell comprising introducing into the cell an isolated or recombinant polynucleotide comprising a miRNA precursor backbone comprising a first polynucleotide segment encoding a miRNA and a second polynucleotide segment encoding a star sequence, wherein: a) said first and said second polynucleotide segments are heterologous to the miRNA precursor backbone; b) said first polynucleotide segment comprises the sequence set forth in SEQ ID NO:4; and, c) said second polynucleotide segment comprises the sequence set forth in SEQ ID NO:8, wherein the expression of said polynucleotide reduces the level of mRNA of each of said at least two sequences relative to the level of mRNA of each of said at least two sequences in the absence of expression of said polynucleotide.