Patent ID: 9631191
Date: 2017-04-25
CPC Classifications: C07K,C12N,C12Q,G01N

Claim:
1. A method for producing a chimeric immunoglobulin-G (IgG) antibody that specifically binds an antigen of interest comprising: a) isolating nucleic acid sequences encoding IgG heavy and light chain variable regions from a single immune cell producing an IgG that specifically binds the antigen of interest; b) cloning the light chain variable region nucleic acid sequences of part a) into the expression vector pVLentry-Hyg10, and cloning the heavy chain variable region nucleic acid sequences of part a) into an expression vector selected from the group consisting of pVHentry-Cm5, pVHentry-GFP1, pVHentry-MLuc7, pVHentry-Hisbio1, and pVHentry-CBD1; wherein the light chain variable region nucleic acid sequences are operably linked to nucleic acid sequences encoding a light chain constant region, and wherein the heavy chain variable region nucleic acid sequences are operably linked to nucleic acid sequences encoding a heavy chain constant region; c) combining the vectors used for part b) into a single vector comprising nucleic acid sequences encoding the IgG heavy and light chain variable regions; d) introducing the expression vector of part c) into a host cell; e) establishing one or more stable cell lines from the host cell of part d); and f) isolating the IgG produced by the one or more stable cell lines of part e).