Patent ID: 9617592
Date: 2017-04-11
CPC Classifications: C12Q,G01N

Claim:
1. A method for identifying a candidate protein, or a DNA encoding the candidate protein, said method comprising culturing said micro-organism under at least two different culture conditions, selecting from said different culture conditions at least two cultures in which the level of a product that is produced by said micro-organism is different than the other culture, preparing a protein and/or RNA sample from the selected cultures of micro-organisms, determining a sequence of at least part of the proteins and/or RNA in said samples, selecting sequences of proteins and/or sequences of RNA of which the amount differs between the samples of the selected cultures of micro-organisms, grouping selected sequences of proteins and/or sequences of RNA coded for by DNAs into a first group that comprises selected sequences that are separated by no more than 30 open reading frames (ORFs) on the genome of the micro-organism, grouping remaining selected sequences of proteins and/or sequences of RNA coded for by DNAs (if any) into a second group that comprises selected sequences that are separated by no more than 30 ORFs on the genome of the micro-organism group, identifying a group of selected sequences that contains the coding regions of at least two different RNAs or proteins of which the amount correlates with the level of the product that is produced by said micro-organism under said at least two different culture conditions, and identifying a protein or DNA that comprises a sequence of the identified group thereby identifying a candidate protein that is likely involved in the production of the product by said micro-organism.