Patent ID: 9696320
Date: 2017-07-04
CPC Classifications: C07K,G01N

Claim:
1. A method for determining whether a test subject afflicted with lung cancer is afflicted with small cell lung cancer comprising: (i) detecting a lung cancer differential marker glycoprotein glycosylated with a glycan at an asparagine residue or at least one fragment thereof in a sample obtained from the test subject, and (ii) determining whether the test subject is afflicted with small cell lung cancer by determining whether the glycoprotein or the fragment thereof is present in the sample; wherein the presence of the glycoprotein or fragment in the sample indicates that the test subject is afflicted with small cell lung cancer, and wherein: (a) the lung cancer differential marker glycoprotein is secretogranin III, and the glycoprotein and the fragment thereof comprise an amino acid sequence selected from the group consisting of SEQ ID NO: 56 and SEQ ID NO: 57, wherein SEQ ID NO: 56 is glycosylated with a glycan at the asparagine residue at position 13 and SEQ ID NO: 57 is glycosylated with a glycan at the asparagine residue at position 6; (b) the lung cancer differential marker glycoprotein or the fragment thereof is detected using at least one glycan probe that binds to a fucosylated glycan, a high mannose-type glycan, a hybrid-type glycan, or a biantennary complex-type glycan, wherein the glycan probe is AAL lectin and/or ConA lectin, an antibody, or a phage antibody; and (c) the sample is a body fluid, lung cancer tissue or a lung lavage.