Patent ID: 9632033
Date: 2017-04-25
CPC Classifications: G01N,G02B,G06K,G06T

Claim:
1. A method to determine a presence of a known nucleic acid sequence of a known molecule in a sample in which the sample contains a targeted nucleic acid sequence, the method comprising: coating a sample surface with a select segment of the known nucleic acid sequence of the known molecule, wherein the sample surface is configured to hold a liquid medium, wherein the select segment of the known nucleic acid sequence of the known molecule ranges from 8 to 40 bases; coating a plurality of beads with a first molecule, wherein a number of the plurality of beads ranges from 100 to 1000 and a size of the plurality of beads ranges from 0.2 to 3.0 microns, wherein the plurality of beads are provided to be sufficient for recognition of a Brownian motion; attaching the targeted nucleic acid sequence to a second molecule that is configured to bind to the first molecule, such that the targeted nucleic acid sequence is attached to the plurality of beads via the first and second molecules; placing the sample on the sample surface, the sample including the liquid medium, the plurality of beads, and the targeted nucleic acid sequence being tested for having the known nucleic acid sequence of the known molecule, wherein the select segment of the known nucleic acid sequence of the known molecule is intended to be complementary to the targeted nucleic acid sequence; video monitoring the Brownian motion of the plurality of beads with an optical device; determining whether the Brownian motion of the plurality of beads in the liquid medium is restricted or not restricted over time; in response to determining that the Brownian motion of the plurality of beads is restricted and in response to the plurality of beads being tethered to the sample surface when the select segment of the known nucleic acid sequence of the known molecule is complementary to the targeted nucleic acid sequence, recognizing the presence of the known nucleic acid sequence in the sample, thus indicating that the targeted nucleic acid sequence is the known nucleic acid sequence; and in response to determining that the Brownian motion is not restricted and in response to the plurality of beads not being tethered to the sample surface when the select segment of the known nucleic acid sequence of the known molecule is not complementary to the targeted nucleic acid sequence, recognizing that the known nucleic acid sequence is not present in the sample, thus indicating that the targeted nucleic acid sequence is not the known nucleic acid sequence: wherein determining whether the Brownian motion of the plurality of beads is restricted or not restricted over time includes: wherein comparing the bead motion tracked for e plurality of beads to the free Brownian motion includes: