Patent ID: 9683001
Date: 2017-06-20
CPC Classifications: C07F,C12N,C12P,C12Y

Claim:
1. A process for the preparation of a phosphinothricin product or precursor thereof, the process comprising: (a) reacting a compound of Formula III with a cyanide source and an ammonia source, thereby forming a nitrile-containing substrate of Formula IV; (b) reacting the nitrile-containing substrate of Formula IV with one or more formylation reagents, thereby producing an N-formyl substrate of Formula V; (c) contacting in a reaction mixture the N-formyl substrate of Formula V with an enzyme capable of catalyzing the hydrolysis of —CN to —COX, wherein X is either —OH or —NH wherein R 3 is hydrogen, substituted or unsubstituted C 1 -C 8 alkyl, substituted or unsubstituted aryl, or an agronomically acceptable salt-forming cation; and (d) hydrolyzing the compound of Formula VII to form a phosphinothricin product or precursor thereof.