Patent ID: 9695422
Date: 2017-07-04
CPC Classifications: A61K,A61P,C07K,C12N

Claim:
1. An expression vector comprising: a first nucleic acid insert operably linked to a promoter, wherein the first insert encodes a Granulocyte Macrophage Colony Stimulating Factor (GM-CSF) cDNA sequence; and a second nucleic acid insert operably linked to the promoter, wherein the second insert encodes a bi-functional short hairpin RNA (bi-shRNA), wherein the bi-shRNA comprises: (a) a first stem loop structure comprising (i) a first guide sequence capable of hybridizing to a region of a mRNA transcript corresponding to base sequences 300-318, 731-740, 1967-1991, 2425-2444, 2827-2851, or 2834-2852 of SEQ ID NO:2; and (ii) a first passenger sequence fully complementary to the first guide sequence; and (b) a second stem loop structure comprising (i) a second guide sequence capable of hybridizing to a region of an mRNA transcript corresponding to base sequences 300-318, 731-740, 1967-1991, 2425-2444, 2827-2851,or 2834-2852 of SEQ ID NO:2; and (ii) a second passenger sequence partially complementary to the second guide sequence.