Patent ID: 9599808
Date: 2017-03-21
CPC Classifications: G02B

Claim:
1. A microscope apparatus comprising: a light source; an illumination optical system configured to illuminate a specimen with light from the light source, the illumination optical system having a spatial light modulation element capable of adjusting a light intensity distribution; an imaging optical system configured to image light from the specimen; a solid-state imaging device configured to generate an image of the specimen based on light from the imaging optical system; and a controller capable of adjusting the spatial light modulation element, the controller being configured to, based on a first image output from the solid-state imaging device when the specimen is illuminated with first illumination light, adjust the spatial light modulation element and illuminate the specimen with second illumination light, wherein the controller performs a calculation so as to convert the first illumination light into the second illumination light, the spatial light modulation element adjusts, based on a result of the calculation, a light intensity distribution of illumination light with which the specimen is irradiated, the controller converts the first image into a binarized image using a Fourier transform, then performs a principal component analysis of the binarized image, and thereby obtains a spatial frequency distribution included in the first image, and the spatial light modulation element adjusts, based on the spatial frequency distribution of the first image, light intensity distributions in two directions perpendicular to an optical axis.