Patent ID: 9605308
Date: 2017-03-28
CPC Classifications: B01L,C12Q

Claim:
1. An apparatus for sequencing a polynucleotide strand, comprising: a flow chamber for receiving flows of different dNTP reagents; multiple reservoirs that each contain a different dNTP reagent; flow paths from each of the reservoirs to the flow chamber; and a fluidics controller that controls the flow from the reservoirs to the flow chamber, wherein the fluidics controller is programmed to successively provide flow from the multiple reservoirs to the flow chamber according to a predetermined ordering, wherein the predetermined ordering comprises an alternate ordering which is not a continuous and ordered repeat of four different dNTP reagent flows, the alternate ordering comprising a same number of flows for each of four different dNTP reagent flows, a flow of a nucleotide N, which is followed directly by a flow of a different nucleotide W, which is followed directly by a flow of the same nucleotide N, wherein the N-W-N flow is further followed directly by a flow of a nucleotide Z that is different from N and W, and the alternate ordering being successively repeated at least once.