Patent ID: 9746476
Date: 2017-08-29
CPC Classifications: C12Q,G01N

Claim:
1. A method of monitoring passage of a reaction component across a portion of an amphipathic aggregate, the method comprising: a) immobilizing a single amphipathic aggregate at a reaction site, wherein at least one molecule is imbedded in the single amphipathic aggregate and wherein the single amphipathic aggregate comprises an aggregate-confined reaction volume localized within a micelle, within a liposome, or within a structural confinement covered by a lipid bilayer: b) exposing the single amphipathic aggregate to a reaction mixture comprising a labeled reaction component, wherein the labeled reaction component binds the imbedded molecule, wherein the binding results in the labeled molecule being transported into the aggregate-confined reaction volume, and wherein the labeled reaction component comprises a detectable label that emits a signal when present in the aggregate-confined reaction volume; and c) detecting the signal at the reaction site, wherein the signal at the reaction site is indicative that the labeled reaction component has passed into the aggregate-confined reaction volume, thereby monitoring the passage of the labeled reaction component across a portion of the amphipathic aggregate.