Patent ID: 9644232
Date: 2017-05-09
CPC Classifications: B01L,C12N,C12Q

Claim:
1. A method for amplification of circulating nucleic acids that are present in a non-cellular fraction of a biological sample, the method comprising: filtering the biological sample to separate the non-cellular fraction from intact cells; collecting the separated, non-cellular fraction onto a dry solid matrix; extracting the circulating nucleic acids from the collected, non-cellular fraction; circularizing the extracted, circulating nucleic acids to form single-stranded nucleic acid circles; and amplifying the single-stranded nucleic acid circles via random-primed rolling circle amplification using completely random primers comprising a nucleotide analogue to form an amplified, circulating nucleic acid product, wherein the circularizing, and amplifying steps take place in a single reaction vessel without any intervening isolation or purification steps.