Patent ID: 9670457
Date: 2017-06-06
CPC Classifications: B33Y,C12N

Claim:
1. A method, comprising: a) obtaining a first umbilical cord tissue from a first newborn animal; b) cleaning said first umbilical cord tissue with a sterilizing agent; c) mechanically dissociating said first umbilical cord tissue to isolate mixed cells from said first umbilical cord tissue without separating vessels from the rest of the cord tissue, wherein said cells are a mixture of differentiated cells, stem cells and progenitor cells; d) obtaining a second umbilical cord tissue from said first newborn animal or from a second newborn animal; e) cutting said second umbilical cord tissue to a desired shape without separating vessels from the rest of the cord tissue and treating said second umbilical cord tissue to produce a decellularized tissue; f) culturing said mixed cells together with said decellularized tissue such that said decellularized tissue provides a 3D scaffold for 3D culturing of said mixed cells, wherein said 3D culturing is in a 0.1% to 5% oxygen environment at 37° C. in a medium supplemented with serum, plasma or platelet lysate from said first newborn animal.