Patent ID: 9738929
Date: 2017-08-22
CPC Classifications: C12N,C12Q,G01N,G16B

Claim:
1. A method of determining a nucleotide sequence of a region of interest in a polynucleotide, the method comprising: introducing a polynucleotide comprising a region of interest to a sequence analysis system comprising a nanopore in a membrane, wherein the polynucleotide comprises a double-stranded portion comprising complementary strands of the region of interest; applying a voltage across the membrane; monitoring variations in ionic current through the nanopore of the sequence analysis system during enzyme chaperone-regulated passage of the polynucleotide through the nanopore; analyzing the monitored variations in ionic current to obtain nucleotide sequence information for the polynucleotide, wherein the nucleotide sequence information comprises redundant sequence information for the region of interest, wherein the redundant sequence information comprises the nucleotide sequence of the complementary strands; and determining a consensus sequence for the region of interest based on the redundant sequence information.