Patent ID: 9689036
Date: 2017-06-27
CPC Classifications: C12Q

Claim:
1. A method for detecting differential expression of miRNA in a human subject comprising: a) hybridizing miRNAs isolated from a platelet fraction of blood to complementary oligonucleotides, wherein the isolated miRNAs include a panel of miRNA biomarkers consisting of pl-hsa-let-7i-star_st, pl-hsa-miR-1271_st, pl-hsa-miR-1274b_st, pl-hsa-miR-1296_st, pl-hsa-miR-138_st, pl-hsa-miR-140-3p_st, pl-hsa-miR-143-star_st, pl-hsa-miR-146b-3p_st, pl-hsa-miR-150-star_st, pl-hsa-miR-181b_st, pl-hsa-miR-188-5p_st, pl-hsa-miR-20b-star_st, pl-hsa-miR-22_st, pl-hsa-miR-27a-star_st, pl-hsa-miR-31_st, pl-hsa-miR-330-3p_st, pl-hsa-miR-345_st, pl-hsa-miR-362-5p_st, pl-hsa-miR-378_st, pl-hsa-miR-378-star_st, pl-hsa-miR-422a_st, pl-hsa-miR-500_st, pl-hsa-miR-501-5p_st, pl-hsa-miR-532-5p_st, pl-hsa-miR-550-star_st, pl-hsa-miR-720_st, pl-hsa-miR-769-3p_st, pl-hsa-miR-769-5p_st, pl-hsa-miR-874_st, pl-hsa-miR-92a-1-star_st, and pl-hsa-miR-941_st, and wherein the complementary oligonucleotides consist of oligonucleotides complementary to the panel of miRNA biomarkers; b) quantifying hybridization of each of the miRNA biomarkers to the complementary oligonucleotides; and c) detecting an increase in expression of at least 1.4-fold in each of the miRNA biomarkers compared to normal expression levels of the miRNA biomarkers.