Patent ID: 9745590
Date: 2017-08-29
CPC Classifications: A61K,A61P,C12N

Claim:
1. A method for preparing a product circular nucleic acid vector comprising an expression cassette, said method comprising: contacting a parent nucleic acid with a unidirectional site specific recombinase, wherein the parent nucleic acid comprises: (a) an expression cassette that is flanked by sites that are recognized by the unidirectional site specific recombinase; and (b) an expression silencing bacterial DNA sequence, wherein the sites that are recognized by the unidirectional site specific recombinase are positioned between the expression cassette and the expression silencing bacterial DNA sequence, wherein said contacting takes place inside of a bacterial cell or in vitro outside of a cell and is performed under conditions sufficient to produce a product circular nucleic acid vector that is devoid of the expression silencing bacterial DNA sequence and comprises (i) the expression cassette and (ii) a product hybrid sequence of the unidirectional site specific recombinase.