Patent ID: 9657328
Date: 2017-05-23
CPC Classifications: C12N,C12Q

Claim:
1. A method for increasing or decreasing the thermostability or acid tolerance of a microbe, comprising the steps of: mutating a nucleotide sequence which encodes Homoserine o-succinyltransferase (Met A) consisting of the amino acid sequence of SEQ ID NO:41 to obtain a nucleic acid molecule encoding a mutant MetA; transforming a microbial host cell with the nucleic acid molecule encoding the mutant MetA; and culturing the microbial host cell transformed with the nucleic acid molecule encoding the mutant MetA to express the mutant MetA, thereby increasing or decreasing the thermostability or acid tolerance of the transformed microbial host cell, wherein the mutant MetA comprises the substitution mutation of: (a) Thr for Ser at position 61 of SEQ ID NO:41; (b) Val for Glu at position 213 of SEQ ID NO:41; (c) Thr for Ile at position 229 of SEQ ID NO:41; (d) Asp for Asn at position 267 of SEQ ID NO:41; (e) Lys for Asn at position 271 of SEQ ID NO:41; (g) Val for Leu at position 110 of SEQ ID NO:41; (h) Leu for Ile at position 124 of SEQ ID NO:41; (i) Leu for Arg at position 160 of SEQ ID NO:41; (j) Thr for Ala at position 195 of SEQ ID NO:41; (k) Glu for Ala at position 200 of SEQ ID NO:41; (l) Gly for Asp at position 218 of SEQ ID NO:41; (m) Tyr for Ile at position 229 of SEQ ID NO:41; or (n) Tyr for Phe at position 247 of SEQ ID NO:41.