Patent ID: 9645140
Date: 2017-05-09
CPC Classifications: A61K,C07K,C12P,C12Y,G01N,Y10T

Claim:
1. A method of detecting an azido modified protein or peptide, comprising: a) forming an azide-alkyne cycloaddition reaction mixture by adding the followings in a reaction mixture: b) incubating the azide-alkyne cycloaddition reaction mixture for a sufficient amount of time to form a protein or peptide-reporter molecule conjugate; c) separating the protein or peptide-reporter molecule conjugate by size and/or weight of the protein or peptide-reporter molecule conjugate to form a separated protein or peptide-reporter molecule conjugate; d) illuminating the separated protein or peptide-reporter molecule conjugate with an appropriate wavelength to form an illuminated protein or peptide-reporter molecule conjugate; e) observing the illuminated protein or peptide-reporter molecule conjugate wherein the azido modified protein or peptide is detected.