Patent ID: 9733257
Date: 2017-08-15
CPC Classifications: C12Q,G01N

Claim:
1. A method to detect osteoarthritis in a human subject comprising: (a) using a protein detection method to determine the levels of three or more different osteoarthritis marker polypeptides in a synovial fluid sample collected from a first human subject, wherein the three or more different osteoarthritis marker polypeptides comprise MCP1, IL8, and KC; (b) using the protein detection method to determine the levels of three or more different osteoarthritis marker polypeptides in a biological fluid sample collected from a second human control subject, wherein the three or more different osteoarthritis marker polypeptides comprise MCP1, IL8, and KC; (c) using the level of each of the three or more different osteoarthritis marker polypeptides in the first human subject and the second human control subject to calculate a probability of the presence of osteoarthritis in the first human subject, and determining whether the probability is greater than or equal to at least about 80%; and (d) when the probability from step (c) is greater than or equal to at least about 80%, determining that osteoarthritis is present in the first human subject.