Patent ID: 9657320
Date: 2017-05-23
CPC Classifications: C12N,C12P,C12Y

Claim:
1. A ketoreductase polypeptide capable of converting substrate N-methyl-3-keto-3-(2-thienyl)-1-propanamine to product (S)—N-methyl-3-hydroxy-3-(2-thienyl)-1-propanamine at a rate that is improved over a reference polypeptide having the amino acid sequence of SEQ ID NO:6, wherein the polypeptide has an amino acid sequence that is at least 91% identical to a reference sequence of SEQ ID NO:6, comprising a substitution at position 190 and at least one further substitution at one or more positions selected from 40, 46, 60, 64, 94, 108, 145, 152, 153, 157, 196, 198, 199, 226, 245, or 249, wherein the residue corresponding to residue X190 is cysteine or proline; X46 is arginine; residue X60 is isoleucine; residue X64 is valine; residue X108 is histidine; residue 152 is asparagine; residue X153 is valine; residue X157 is serine; residue X198 is asparagine; residue X199 is aspartic acid, valine, arginine, and/or phenylalanine; and/or residue X245 is isoleucine.