Patent ID: 9545107
Date: 2017-01-17
CPC Classifications: A01N,C07K,C12Q

Claim:
1. A process for preparation of Allium fistulosum leaf agglutinin (AFAL) recombinant protein by amplifying the AFAL gene, wherein the process comprises: a) extracting total RNA from b) synthesizing cDNA from total RNA extracted from the leaves of c) employing primers with a nucleotide sequence as set forth in SEQ ID NO:5 and SEQ ID NO:6 with cDNA of (b) to amplify and obtain a nucleotide fragment encoding full-length AFAL protein; d) employing primers having nucleotide sequences as set forth in SEQ ID NO:7 and SEQ ID NO:8 with said nucleotide fragment to amplify and obtain a polynucleotide fragment encoding mature AFAL protein; e) obtaining an expression cassette comprising said polynucleotide fragment and a promoter; f) introducing said expression cassette in a host to obtain recombinant host cells; and g) culturing said recombinant host cells to obtain recombinant AFAL protein.