Patent ID: 9708632
Date: 2017-07-18
CPC Classifications: C07C,C12N,C12P,C12Y,Y02E

Claim:
1. A method for producing 1,3-BDO comprising culturing a non-naturally occurring microbial organism under conditions and for a sufficient period of time to produce 1,3-BDO; said non-naturally occurring microbial organism comprising at least two exogenous nucleic acids each encoding a 1,3-BDO pathway enzyme expressed in a sufficient amount to produce 1,3-BDO; said 1,3-BDO pathway enzyme comprising an acetoacetyl-CoA reductase (ketone reducing) that converts acetoacetyl-CoA to 3-hydroxybutyryl-CoA; and (i) a 3-hydroxybutyryl-CoA reductase (aldehyde forming) that converts 3-hydroxybutyryl-CoA to 3-hydroxybutyraldehyde; or (ii) a 3-hydroxybutyraldehyde reductase that converts 3-hydroxybutyraldehyde to 1,3-butanediol.