Patent ID: 9574229
Date: 2017-02-21
CPC Classifications: C12Q,G16B

Claim:
1. An in vitro method for detecting allelic imbalance at a plurality of single nucleotide polymorphism loci, comprising: (1) extracting DNA from a formalin-fixed paraffin-embedded sample comprising at least one tumor cell obtained from a patient to produce at least one solution comprising genomic DNA of the at least one tumor cell; (2) enriching the at least one solution in (1) for test DNA molecules each comprising at least one locus from the plurality of single nucleotide polymorphism loci, wherein the plurality of single nucleotide polymorphism loci comprises at least 1,000 single nucleotide polymorphism loci, wherein there is at least one single nucleotide polymorphism locus from the plurality of single nucleotide polymorphism loci located on average every 5Mb within each chromosome analyzed, and wherein enriching the at least one solution comprises: (3) performing a DNA sequencing reaction on the at least one enriched solution in (2) to detect the genotype of each locus in the plurality of single nucleotide polymorphism loci; (4) determining the lengths of a plurality of regions of allelic imbalance in the genome of the at least one tumor cell by detecting a plurality of stretches of single nucleotide polymorphism loci in which allelic imbalance is detected in (3).