Patent ID: 9582877
Date: 2017-02-28
CPC Classifications: G01N,G06K,G06T

Claim:
1. An array reader system comprising a computer system comprising a digital processor configured to execute computer-executable instructions to at least: (a) acquiring image data of a region of an array, wherein the array comprises a plurality of stochastically labeled and non-labeled features, and wherein the features comprise oligonucleotide probes; (b) measuring a signal intensity and a local background intensity for one of the plurality of stochastically labeled and non-labeled features based on the acquired image data; (c) calculating a local background corrected signal intensity for the one of the plurality of stochastically labeled and non-labeled features using the signal intensity and the local background intensity measured in (b); (d) transforming the local background corrected signal intensity calculated in (c) for the one of the plurality of stochastically labeled and non-labeled features to determine the number of stochastically labeled and non-labeled features in the of the array; and (e) calculating the absolute number of a target molecule based on the number of stochastically labeled and non-labeled features determined in (d) within the region of the array.