Patent ID: 9701968
Date: 2017-07-11
CPC Classifications: A61L,C12M,C12N,C12Q,C12Y

Claim:
1. A sterilization monitor, comprising: a first compartment containing a biological indicator, the biological indicator comprising: a host organism comprising a spore forming bacteria from the group consisting of Geobacillus stearothermophilus, Bacillus atrophaeus, Bacillus spaericus, Bacillus anthracis, Bacillus pumilus, Bacillus coagulans, Bacillus subtilid globigii, Bacillus cereus, Bacillus circulans , a reporter gene for producing an indicator enzyme, a regulatory gene, and a vehicle for inserting the reporter gene and the regulatory gene in the host organism, the host organism bearing a transposable genetic element in its genome for inserting an insertion sequence in the regulatory gene, the insertion sequence comprising an IS21 family insertion sequence from the group consisting of IS21, IS53, IS232A, IS408, IS1162, IS1326, IS1415, or IS5376, wherein the vehicle is taken up by the host organism, the insertion sequence is inserted in the regulatory gene, and the host organism undergoes sporulation, the first compartment being adapted to permit the biological indicator to be brought into contact with a sterilization medium during a sterilization process; and a second compartment containing a recovery medium, the second compartment being adapted to maintain the recovery medium separate from the biological indicator during the sterilization process, and to permit the recovery medium to contact the biological indicator after the biological indicator has been exposed to the sterilization medium.