Patent ID: 9689030
Date: 2017-06-27
CPC Classifications: C12Q,G01N

Claim:
1. A kit for the detection of a primer extension product produced in the presence of a polymerase lacking 3′→5′ exonuclease activity, that comprises: 1) at least two single-labelled oligonucleotide sequences that hybridize to one another in free solution to form a fluorescent quenched pair, that upon introduction of a complementary sequence to one or both sequences generates a measurable signal when the complementary sequence is hybridized to one of the at least two single-labelled oligonucleotide sequences; and 2) a polymerase lacking 3′→5′ exonuclease activity; wherein at least one of the internal bases of at least one of the oligonucleotide sequences is modified by a phosphorothioate group and wherein: the at least one oligonucleotide sequence that contains at least one phosphorothioate group has 20-80% of the bases modified by a phosphorothioate group.