Patent ID: 9732379
Date: 2017-08-15
CPC Classifications: C12Q,G01N

Claim:
1. A set of probe molecules comprising at least a first probe molecule and a second probe molecule each comprising: a) a capture domain that comprises a sequence with complementarity to a target nucleic acid; b) a terminal extension that is covalently linked to the 5′ end, the 3′ end, or both the 5′ end and the 3′ end of the capture domain; and c) at least one polymer label branched from an internal residue located within at least one of the terminal extension(s), wherein the nucleic acid capture domain of the first probe molecule comprises a sequence with complementarity to first target nucleic acid and the nucleic acid capture domain of the second probe molecule comprises a sequence with complementarity to a second target nucleic acid, wherein the polymer label is a polyglycol selected from the group consisting of polyethylene glycol (PEG), methoxypolyethylene glycol (MPEG), polypropylene glycol (PPG), polybutylene glycols (PBG), and copolymers thereof wherein the polymer label of the first probe molecule is different from the polymer label of the second probe molecule, and wherein the polymer labels of the first and second probe molecules provide for independent detection of the first and second target nucleic acids in a nanopore system.