Patent ID: 9587263
Date: 2017-03-07
CPC Classifications: C12P,C12Q

Claim:
1. A method for amplifying a nucleic acid, comprising: a) providing a target nucleic acid template; b) contacting the target nucleic acid template with a reaction mixture comprising a DNA polymerase having a strand displacement activity, a deoxyribonucleoside triphosphate (dNTP) mixture, a primer with a 3′ end and a 5′ end, a molecular crowding reagent, and a buffer solution, wherein the buffer solution maintains a salt concentration of the reaction mixture between 1 to 75 mM; and c) amplifying the target nucleic acid template under isothermal amplification conditions at a constant reaction temperature, wherein the salt concentration results in a melting temperature (T wherein the molecular crowding reagent is polyethylene glycol (PEG).