Patent ID: 9709468
Date: 2017-07-18
CPC Classifications: C12N,G01N

Claim:
1. A sample preparation device comprising: a filter member including a filter which separates cells being an analysis target from other component in a sample, the sample comprising the cells which are the analysis target, the component other than the analysis target and a first liquid, wherein the filter member comprises an indented cylindrical portion forming a surrounding cylindrical inner side face, and the filter is disposed within the indented cylindrical portion displaced at a defined distance away from an top surface of the filter member; a first receptacle and a second receptacle connected to each other via the filter member which is disposed between the first receptacle and the second receptacle, a communication hole being formed within a recess cavity of the first receptacle and allowing the sample to go into and out of the first receptacle, wherein the recess cavity of the first receptacle fully contains a rotating member having a rotating surface which comprises an inner circular portion of a first diameter and a restraining portion concentrically formed around the inner circular portion with a second diameter wherein the second diameter is larger than the first diameter, wherein the inner circular portion is formed on top of the restraining portion such that when the inner circular portion is inserted into the indented cylindrical portion, the restraining portion restrains the inner circular portion from coming into contact with the filter, wherein a protrusion portion is formed on the inner circular portion, and the protrusion portion when rotated with the rotating member which is contained within the recess cavity, facilitates removal of the cells in the sample collected on a surface of the filter facing the recess cavity; a third receptacle for holding the sample therein; a flow path which allows the third receptacle to be communicated with the communication hole; a negative pressure section which applies a negative pressure into the second receptacle, thereby to move the sample in the third receptacle toward the filter via the flow path and the first receptacle, and thereby to move the component other than the analysis target into the second receptacle via the filter; and a positive pressure section which applies a positive pressure from the second receptacle side to the filter to which the cells are attached; and a liquid supply part for supplying a second liquid which is different from the first liquid to the first receptacle and the second receptacle, wherein the negative pressure section operates in a state where both the first receptacle and the second receptacle are filled with the second liquid by the liquid supply part, to move the component other than the analysis target and the first liquid from the first receptacle to the second receptacle via the filter.