Patent ID: 9636384
Date: 2017-05-02
CPC Classifications: A61K,A61P,B82Y

Claim:
1. A method for increasing the serum half-life of an enzymatic polypeptide, wherein said method comprises; attaching said enzymatic polypeptide to a polymeric nanoparticle to form a complex, wherein said attached enzymatic polypeptide has a longer serum half-life than a control enzymatic polypeptide not attached to a polymeric nanoparticle, wherein said enzymatic polypeptide is attached to said polymeric nanoparticle via one or more attachment points, and wherein said polymeric nanoparticle is in the form of a micelle; wherein said enzymatic polypeptide is selected from the group of polypeptides consisting of: a galactocerebrosidase, beta-glucosidase, ceramidase, alpha-L-iduronidase, maltase, sphingomyelinase, hexosaminidase, sulfamidase, and arylsulfatase A; wherein said polymeric nanoparticle is assembled from COOH-PEG-PLA, methoxy-PEG-PLA polymers, or maleimide-PEG-PLA polymers, or any combination thereof; and wherein said one or more attachment points is independently a N-terminus, a C-terminus, or a tag on the side chain of said enzymatic polypeptide.