Patent ID: 9670523
Date: 2017-06-06
CPC Classifications: C12Q,G01N

Claim:
1. A method for detecting the severity of tuberculosis in humans comprising: (a) providing sputum from said subject; (b) adding reagents A and B to the sputum obtained in step (a) to obtain a solution; (c) detecting color intensity of the solution obtained in step (b), wherein a detected color of magenta indicates that the disease is most severe while a detected color of faintly pink indicates that it is least severe; and (d) detecting the titre of bacilli present in the solution obtained in step (b), wherein the color level is determined by comparing with color coded strips or standard color codes wherein the reagent A is sulphanilic acid and the reagent B is N-(1-naphthyl)ethylenediamine dihydrochloride (NEDD).