Patent ID: 9688752
Date: 2017-06-27
CPC Classifications: A61K,C07K

Claim:
1. A method for producing a low acidic species composition comprising an anti-TNFα antibody, or antigen-binding portion thereof, wherein the anti-TNFα antibody, or antigen-binding portion thereof, comprises a light chain variable region (LCVR) having a CDR1 domain comprising the amino acid sequence of SEQ ID NO: 7, a CDR2 domain comprising the amino acid sequence of SEQ ID NO: 5, and a CDR3 domain comprising the amino acid sequence of SEQ ID NO: 3; and a heavy chain variable region (HCVR) having a CDR1 domain comprising the amino acid sequence of SEQ ID NO: 8, a CDR2 domain comprising the amino acid sequence of SEQ ID NO: 6, and a CDR3 domain comprising the amino acid sequence of SEQ ID NO: 4, the method comprising: (a) contacting a first composition comprising the anti-TNFα antibody, or antigen-binding portion thereof with a chromatography media, wherein the first composition comprises more than 10% acidic species of the anti-TNFα antibody, or antigen-binding portion thereof, and wherein the anti-TNFα antibody, or antigen-binding portion thereof binds to the chromatography media; (b) displacing the anti-TNFα antibody, or antigen-binding portion thereof, bound to the chromatography media with a displacing buffer; and (c) collecting a second composition comprising the displaced anti-TNFα antibody, or antigen-binding portion thereof, wherein the second composition comprises less than 10% acidic species of the anti-TNFα antibody, or antigen-binding portion thereof, thereby producing a low acidic species composition.