Patent ID: 9625474
Date: 2017-04-18
CPC Classifications: G01N

Claim:
1. A method of detecting differential levels of fragments of phagocytosed Aβ peptide in a sample obtained from a patient comprising the steps of: (i) subjecting a sample selected from a cerebro-spinal fluid sample and a blood sample obtained from the patient to cell sorting to isolate activated macrophages that display CD14 and CD16 cell surface markers; (ii) lysing said activated macrophages and immunoprecipitating any Aβ peptide fragments obtained from said lysed activated macrophages, wherein said Aβ peptide fragments result from intracellular degradation of phagocytosed Aβ peptide proteins; and (iii) detecting the level of said Aβ peptide fragments immunoprecipitated from said activated macrophages.