Patent ID: 9566236
Date: 2017-02-14
CPC Classifications: A61K

Claim:
1. A method for producing a proteoliposome by mixing lipid, membrane proteins and a surfactant to prepare a mixed solution, then removing the surfactant from the mixed solution, wherein the content of the surfactant in the mixed solution is made 2.0 times or more than the sum of a maximum amount of the surfactant associating with the lipid plus a maximum amount of the surfactant associating with the membrane protein, wherein a content of lipid in the mixed solution is 2.2 to 3.0 mM based on the total amount of the mixed solution, wherein a content of the membrane protein in the mixed solution is 83 to 145 nM based on the total amount of the mixed solution, wherein the lipid is at least one selected from the group consisting of phosphatidylcholine and phosphatidylserine, wherein the membrane protein is an ionotropic receptor, and wherein the surfactant is a nonionic surfactant.