Patent ID: 9562911
Date: 2017-02-07
CPC Classifications: A61P,G01N

Claim:
1. A method for characterizing an antibody and complement profile for food immune reactivity in a subject, comprising: obtaining a sample comprising one or more immunoglobulins and native Immunoglobulin-C1q complex from said subject; providing a test surface comprising a food antigen coating, wherein the food antigen coating comprises a first food antigen, a second food antigen, and a third food antigen that have been applied to the test surface in a sequential manner; contacting the test surface with at least a portion of the sample; contacting the test surface with an antibody mixture, wherein the antibody mixture comprises a first antibody directed to C1q and a second antibody directed to at least one of the one or more immunoglobulins of the sample species; and obtaining a signal characteristic of binding of both at least one of the immunoglobulins and native Immunoglobulin-C1q complex from the sample to the test surface, wherein a signal intensity that exceeds a background cutoff is characterized as a positive result.