Patent ID: 9575064
Date: 2017-02-21
CPC Classifications: C12Q,G01N

Claim:
1. A process for detecting enzymatic activity in vitro, comprising: contacting a sample containing a target enzyme with a substrate and one or more oleate salts wherein said salt is selected from the group consisting of sodium, magnesium, potassium, calcium, lithium and zinc, said step of contacting is performed under conditions wherein the target enzyme is lysosomal storage enzyme capable of acting on the substrate to produce an enzymatic product, and wherein said oleate salt allows for improved enzyme activity, detection of lower enzyme amounts, and/or greater reproducibility; and detecting said enzymatic product, wherein said target enzyme is acid α-galactosidase A, acid β-glucocerebrosidase, galactocerebrosidase, acid α-glucosidase, acid sphingomylenase, α-L-iduronidase, or combinations thereof.