Patent ID: 9714446
Date: 2017-07-25
CPC Classifications: C12Q

Claim:
1. A composition comprising at least two tripartite complexes, each tripartite complex comprising: (a) a tag molecule, wherein the tag molecule comprises a first DNA sequence and a plurality of reporter attachment regions and wherein the first DNA sequence comprises a DNA sequence having no greater than 85% identity across 35-50 bases with a maximum continuous homology of 15 bases or less to any known genomic DNA or RNA transcribed from a genome; (b) one or more reporter molecules each non-covalently attached to one of the plurality of reporter attachment regions of the tag molecule, wherein the one or more reporter molecules together produces a code comprising an ordered series of colored fluorescent spots that identifies a target RNA molecule; and (c) a bridge molecule, wherein the bridge molecule comprises a second DNA sequence that is complementary to the target RNA molecule and a third DNA sequence that is complementary to the first DNA sequence of the tag molecule; wherein a first tripartite complex identifies a first target RNA molecule and comprises a bridge molecule that is complementary to the first target RNA molecule; wherein an at least second tripartite complex identifies an at least second target RNA molecule and comprises a bridge molecule that is complementary to the at least second target RNA molecule; and wherein the first and the at least second target RNA molecules are different.