Patent ID: 9624484
Date: 2017-04-18
CPC Classifications: A61K,A61P,C12N,C12Y

Claim:
1. A nucleic acid comprising a nucleotide sequence encoding a modified human cystathionine-γ-lyase (CGL) enzyme having at least the following substitutions relative to a native human CGL amino acid sequence (see SEQ ID NO: 1), said substitutions selected from (a) E59N, S63L, L91M, R119L, K268R, T311G, E339V and I353S; (b) E59I, S63L, L91M, R119L, K268R, T311G, E339V and I353S; (c) E59N, S63L, L91M, R119A, K268R, T311G, E339V and I353S; and (d) E59I, S63L, L91M, R119A, K268R, T311G, E339V and I353S, wherein the modified human CGL enzyme has higher methionine-γ-lyase activity compared to hMGL-NLV.