Patent ID: 9732356
Date: 2017-08-15
CPC Classifications: A61K,A61P,C07K,C12N

Claim:
1. A method for obtaining glucose-regulated expression of insulin ex vivo in mammalian hepatocytes, wherein the method comprises delivering a first, second or third genetic vector for glucose-regulated synthesis of insulin into an isolated mammalian hepatocyte and wherein the insulin levels in the blood of the mammal stays within 0.5 uU-100 uU/ml and the blood glucose concentration of the mammal stays within 80-150 mg/dl for at least 10 days after transplant of the mammalian hepatocytes into a mammal, wherein the first vector comprises SEQ ID NO:1, wherein the second vector comprises SEQ ID NO:2, wherein the third vector comprises SEQ ID NO:3, and wherein glucose-regulated expression of insulin occurs.