Patent ID: 9567629
Date: 2017-02-14
CPC Classifications: C07H,C12N,C12Q,C12Y,G01N

Claim:
1. A method for generating a signal, comprising: contacting a labeled polymerase conjugate with a labeled nucleotide under conditions where the labeled polymerase conjugate catalyzes the incorporation of the labeled nucleotide into a nucleic acid, wherein the labeled polymerase conjugate includes a polymerase linked to at least three optically-detectable dye labels, wherein the at least three optically-detectable dye labels are linked to one or more biotin acceptor peptide sequence of the polymerase, and wherein the polymerase has nucleotide incorporation activity, and wherein the labeled polymerase conjugate emits upon continuous excitation a signal having a total photon count of at least 10 6 photons before irreversibly photo-bleaching.