Patent ID: 9653271
Date: 2017-05-16
CPC Classifications: G01N,H01J

Claim:
1. A method of forming structural information of a sample comprising lipids using mass spectrometry, comprising: optimizing ionization source conditions to produce first generation product ions at the source having substantially maximum intensity; mass selecting, from the first generation product ions, ions having a mass of interest; fragmenting at least a portion of the mass-selected first generation product ions to produce second generation product ions; separating at least a portion of the second generation product ions by mobility; fragmenting a portion of the mobility separated second generation product ions to produce third generation product ions, and maintaining a remaining portion of the mobility separated second generation ions, wherein the third generation product ions are time aligned with the second generation product ions; detecting, by a detector, the third generation product ions and the remaining portion of the mobility separated second generation product ions to form a series of mass spectra comprising time aligned fragmentation patterns, each mass spectrum being associated with a different mobility value of the second generation product ions; and determining the position of a fatty acyl double bond in the lipid from the time aligned fragmentation patterns.