Patent ID: 9739734
Date: 2017-08-22
CPC Classifications: A61K,G01N

Claim:
1. A method of detecting and measuring exocytosis of a target molecule comprising insulin, glucagon, somatostatin, or any combination thereof, from a population of cells that is known or suspected to produce the target molecule, derived from a human subject, the method comprising: a. applying a first electrical signal with a first frequency across the population of cells, the first electrical signal being either an alternating current or an alternating voltage; b. applying a second electrical signal with a second frequency across the population of cells, the second electrical signal being either an alternating current or an alternating voltage; c. measuring a first voltage change across the population of cells at the first frequency if the first electrical signal is an alternating current or measuring a first current change across the population of cells at the first frequency if the first electrical signal is an alternating voltage; d. measuring a second voltage change across the population of cells at the second frequency if the second electrical signal is an alternating current or measuring a second current change across the population of cells at the second frequency if the second electrical signal is an alternating voltage; and e. determining a change in an electrical impedance of the population of cells at each of the first frequency and the second frequency, wherein the changes in the electrical impedances of the population of cells indicate an amount of the target molecule released from the population of cells.