Patent ID: 9671394
Date: 2017-06-06
CPC Classifications: G01N

Claim:
1. An in vitro method for the determination of the presence and/or amount of an antigen of a multispecific antibody in a sample, whereby the antigen to be detected can be specifically bound by a first binding specificity of the multispecific antibody, comprising the steps of: incubating the sample comprising the multispecific antibody, multispecific antibody bound antigen and free antigen with an anti-idiotypic antibody that specifically binds to a second binding specificity of the multispecific antibody, which is different from the first binding specificity, depleting the anti-idiotypic antibody-multispecific antibody-complex and anti-idiotypic antibody-multispecific antibody-antigen complex from the sample prior to the determination of the presence and/or the amount of free antigen, and determining the presence and/or the amount of free antigen in the multispecific antibody-depleted sample.