Patent ID: 9592198
Date: 2017-03-14
CPC Classifications: A61K,B01J

Claim:
1. A method for continuous flow synthesis and active loading of liposomes, comprising the steps of: providing a substrate comprising a sample flow channel having a liposome formation region, a transmembrane gradient formation region, and an agent loading region, wherein said liposome formation region comprises an inlet through which a lipid solution flows and inlets through which a buffer solution flows, wherein said transmembrane gradient formation region is a microdialysis region comprising a counterflow channel adjacent to said sample flow channel, and a membrane in between said sample flow channel and said counterflow channel, said membrane permitting buffer exchange between said sample flow channel and said counterflow channel and establishing a transmembrane ion gradient, and wherein said agent loading region comprises an inlet through which a first agent flows and in fluid connection with said sample flow channel; interacting said lipid solution and said buffer solution within said liposome formation region of said sample flow channel, thereby forming a population of liposomes within said liposome formation region; establishing a liposome transmembrane gradient within said transmembrane gradient formation region of said sample flow channel; and mixing said first agent with said liposomes received from said transmembrane gradient formation region within said agent loading region, thereby actively loading said first agent within intravesicular spaces of said liposomes to form agent-loaded liposomes.