Patent ID: 9534215
Date: 2017-01-03
CPC Classifications: C07H,C12N,C12Q

Claim:
1. A method of separating bead substrates, the method comprising: applying an emulsion to an emulsion-breaking solution, the emulsion breaking solution being an aqueous solution including a surfactant, a dispersed phase of the emulsion comprising an unbound polynucleotide, a first set of bead substrates and a second set of bead substrates, the unbound polynucleotide including a segment complementary to a coupling oligonucleotide, the first set of bead substrates including the coupling oligonucleotide extended to include a segment complementary to a portion of the unbound polynucleotide, the second set of bead substrates including the coupling oligonucleotide, the emulsion-breaking solution including an interference probe having a sequence the same as at least a portion of the coupling oligonucleotide or complementary to the coupling oligonucleotide; binding beads of the first set of bead substrates to separation substrates; and separating unbound beads of the second set of bead substrates from the beads of the first set of bead substrates bound to the separation substrates.