Patent ID: 9728387
Date: 2017-08-08
CPC Classifications: G01N,H01J

Claim:
1. A method of sample processing, comprising: providing a fluidic device with at least one microfluidic or nanofluidic separation channel in fluid communication with a background electrolyte (BGE) reservoir, and a nanofluidic or microfluidic sample channel in fluid communication with the separation channel, wherein the sample channel comprises at least one solid phase extraction (SPE) bed; flowing a sample through the sample channel, across the at least one SPE bed, and into the separation channel; electrophoretically separating an analyte component from the sample in the separation channel; and performing at least one of: wherein the method further comprises: flowing an ion-pairing agent across the at least one SPE bed in advance of, or together with, the sample; rinsing the ion pairing agent from the sample channel into a waste channel in fluid communication with the sample channel; and after rinsing the ion pairing agent from the sample channel, flowing an elution fluid in the sample channel before or during the electrophoretic separation.