Patent ID: 9695417
Date: 2017-07-04
CPC Classifications: C12N,C12P

Claim:
1. A method for synthesizing long-chain nucleic acid molecules through assembling short-chain oligonucleotides, the method comprising: phosphorylating a set of short-chain oligonucleotides; modifying at least one of the short-chain oligonucleotides in the set with a first chemical group; annealing the set of short-chain oligonucleotides; ligating the set of short-chain oligonucleotides in the presence of a ligase, wherein the ligation produces at least one double-stranded long-chain nucleotide sequence; immobilizing the at least one double-stranded long-chain nucleotide sequence on solid particles modified with a second chemical group, wherein the first chemical group and second chemical group have an affinity for one another; purifying the at least one double-stranded long-chain nucleotide sequence, said purifying step including a transcription step followed by a subsequent reverse-transcription step; after purification, amplifying the at least one double-stranded long-chain nucleotide sequence.