Patent ID: 9719129
Date: 2017-08-01
CPC Classifications: B01D,C12N,C12Q

Claim:
1. A method of isolating vesicles from biological fluid, comprising: (a) obtaining a biological fluid sample comprising vesicles having a diameter of less than 0.5 micrometers; (b) loading at least a portion of said biological fluid sample into a sample loading region of a vesicle capture device, said portion of said biological fluid sample comprising blood plasma and vesicles having a diameter of less than 0.5 micrometers; (c) passing said portion of said biological fluid sample from said sample loading region through a vesicle-capture material in said vesicle capture device, said vesicle-capture material comprising at least a first layer and a second layer, both of glassfiber, at least a portion of said vesicles having a diameter of less than 0.5 micrometers adsorbing to and being retained by the vesicle-capture material, thereby producing a supernatant, wherein said first layer is closer to said sample loading region than said second layer, wherein said first layer has a porosity configured to capture material that is 1.6 micrometers or greater in diameter, wherein said second layer has a porosity configured to capture material that is 0.6 micrometers to 0.8 micrometers or greater in diameter; and (d) passing said supernatant to a sample receiving region of said vesicle capture device, thereby isolating vesicles from the biological fluid sample.