Patent ID: 9550972
Date: 2017-01-24
CPC Classifications: C12M

Claim:
1. A bioreactor, comprising: a culture vessel for seeding and culturing cells by adding a cell-culture medium, wherein the culture vessel comprises at least a side wall and a bottom surface; wherein the culture vessel is configured such that a specific heat transfer area (sHTA) and a specific gas transfer area (sGTA) of the culture vessel are constant with respect to a change in a volume of the cell-culture medium in the culture vessel, and wherein the specific heat transfer area at a height h of the cell-culture medium in the culture vessel is defined by equation (1) and the specific gas transfer area at a height h of the cell-culture medium in the culture vessel is defined by equation (2): wherein sHTA(h) is a specific heat transfer area for a height h of the cell-culture medium in the culture vessel, HTA(h) is a heat transfer area for the height h of the cell-culture medium in the culture vessel, V(h) is a volume of the cell-culture medium for the height h of the cell-culture medium in the culture vessel, sGTA(h) is a specific gas transfer area at the height h of the cell-culture medium in the culture vessel, and GTA(h) is an area of a top surface of the cell-culture medium at the height h of the cell-culture medium in the culture vessel.