Patent ID: 9611285
Date: 2017-04-04
CPC Classifications: C07H,C12N,C12P

Claim:
1. A method for producing fucosyllactose comprising the steps of: a) providing a polypeptide with alpha-1,2-fucosyltransferase activity comprising the amino acid sequence shown in SEQ ID NO: 2, wherein the polypeptide can transfer a fucose residue to a monosaccharide or an oligosaccharide that comprises lactose; and b) contacting the polypeptide with alpha-1,2-fucosyltransferase activity of step a) with a mixture comprising a donor substrate, wherein the donor substrate is GDP-fucose, and an acceptor substrate comprising a mono- or oligosaccharide comprising lactose under conditions wherein the polypeptide catalyzes the transfer of a fucose residue from the donor substrate to the acceptor substrate, thereby producing the fucosyllactose.