Patent ID: 9683053
Date: 2017-06-20
CPC Classifications: C07K

Claim:
1. A hetero-dimeric immunoglobulin or hetero-dimeric fragment thereof comprising: (a) a first engineered immunoglobulin chain comprising an engineered parent domain having a protein-protein interface, wherein at least one amino acid residue of the protein-protein interface of the parent domain of said first engineered immunoglobulin chain is substituted with an amino acid residue from a protein-protein interface of a first donor domain; and (b) a second engineered immunoglobulin chain comprising an engineered parent domain having a protein-protein interface, wherein at least one amino acid residue of the protein-protein interface of the parent domain of said second engineered immunoglobulin chain is substituted with an amino acid residue from a protein-protein interface of a second donor domain, wherein the engineered parent domain of the first engineered immunoglobulin chain and the engineered parent domain of the second engineered immunoglobulin chain are not identical and, wherein the first donor domain and the second donor domain form a naturally occurring TCR constant domain hetero-dimer or a naturally occurring TCR constant domain homo-dimer and, wherein the parent domain of the first and/or the second engineered immunoglobulin chain is a CH3 domain, wherein the amino acid residue which is substituted in the protein-protein interface of the parent domain of the first engineered immunoglobulin chain comprises the amino acid residue at position 88, and, wherein the amino acid residue which is substituted in the protein-protein interface of the parent domain of the second engineered immunoglobulin chain comprises the amino acid residue at position 85.1 and/or 86, and wherein the amino acid residue substituted at position 88 in the parent domain of the first engineered immunoglobulin chain is interacting with the amino acid residue substituted at position 85.1 and/or 86 in the parent domain of the second engineered immunoglobulin chain, wherein the amino acid position of each group member is indicated according to the IMGT® numbering.