Patent ID: 9611501
Date: 2017-04-04
CPC Classifications: A61P,C12Q,G01N

Claim:
1. A method for determining sensitivity of bacteria to an antibiotic, the method comprising: (a) immobilizing the bacteria from a bacterial suspension by covalent attachment to a solid support prior to exposing the bacterial suspension to a combination of a stressor and the antibiotic, (b) contacting said immobilized bacteria with an agent comprising a reporter moiety, which preferentially binds to damaged bacterial cells, (c) subjecting said immobilized bacteria to the stressor in the presence of the antibiotic without a bacterial growth phase subsequent to immobilization, (d) detecting a signal from said reporter moiety in said immobilized bacteria after contacting said covalently immobilized bacteria with the stressor in the presence of the antibiotic and without the need for bacterial growth phase subsequent to immobilization, and (e) comparing the signal on the solid support from said immobilized bacteria in the presence of the antibiotic to a control comprising immobilized bacteria subjected to the stressor in the absence of the antibiotic, wherein detection of an increase in said signal in the presence of an antibiotic compared to a control indicates that said bacteria are susceptible to said antibiotic, and wherein a signal that is comparable to the control in the presence of an antibiotic indicates that said bacteria are resistant to said antibiotic.