Patent ID: 9670525
Date: 2017-06-06
CPC Classifications: C12Q,G01N

Claim:
1. A method for measuring cholesterol in high-density lipoprotein in a specimen, comprising (1) reacting the specimen with i) a cholesterol ester hydrolase and a cholesterol oxidase, or ii) a cholesterol ester hydrolase, an oxidized coenzyme, and a cholesterol dehydrogenase, in an aqueous medium comprising a polyanion, and at least one substance selected from the group consisting of a substance represented by formula (I): wherein R wherein R (2) measuring the formed hydrogen peroxide or reduced coenzyme; and (3) calculating the concentration of HDL cholesterol in the specimen from the value measured in (2) and a previously-prepared calibration curve showing the relationship between HDL cholesterol concentrations and measured values of hydrogen peroxide or reduced coenzyme.