Patent ID: 9598675
Date: 2017-03-21
CPC Classifications: A61K,C12N

Claim:
1. A method for preparing induced pluripotent stem cell-derived mesenchymal stem cells and induced pluripotent stem cell-derived cardiomyocyte cells comprising the steps of: (i) providing one or more induced pluripotent stem cells; (ii) forming one or more embryoid bodies from the induced pluripotent stem cells using a non-adherent substrate; (iii) contacting the embryoid bodies with serum-free and insulin-free medium comprising a p38-MAPK inhibitor to form aggregates of contracting embryoid body cells; (iv) transferring at least one contracting embryoid body aggregate to a cell-culture treated substrate and contacting the contracting embryoid body aggregates with a medium comprising ≦5% serum to form a population of cells comprising induced pluripotent stem cell-derived mesenchymal stem cells and aggregates of induced pluripotent stem cell-derived cardiomyocyte cells, wherein the population of cells is substantially adhered to the substrate.