Patent ID: 9631188
Date: 2017-04-25
CPC Classifications: A61K,A61P,C12N,C12Y

Claim:
1. A truncated recombinant human cystathionine beta-synthase (CBS) protein having enzymatic activity prepared by a process comprising: a) transfecting a recombinant host cell with a recombinant expression vector, said expression vector comprising: b) culturing the transfected host cell from (a) under conditions effective to produce the truncated recombinant human CBS protein or homologue thereof comprising the fusion segment in the cell culture media; and c) contacting the truncated recombinant human CBS protein or homologue thereof comprising the fusion segment of (b) with a rhinovirus 3C protease to cleave the fusion segment from the recombinant CBS protein or homologue thereof to produce the truncated recombinant human CBS protein or homologue thereof having two C-terminal amino acid residues of the rhinovirus 3C protease recognition site, wherein the nucleic acid sequence encoding the truncated recombinant human CBS protein or homologue thereof encodes an amino acid sequence having an amino terminal or a carboxyl terminal deletion or both, wherein the amino acid sequence of the truncated recombinant human CBS protein spans from a starting position of residues 1-83 of SEQ ID NO: 2 to an ending position of residues 382-550 of SEQ ID NO: 2.