Patent ID: 9638689
Date: 2017-05-02
CPC Classifications: C07K,G01N

Claim:
1. A method for analyzing simultaneously multiple human antigen-specific cell populations of a sample, the sample comprising B cells and antigen-specific cells, the method comprising a) separation of B cells from said sample b) dividing the B cells into n sub-samples c) differentially labeling the B cells of said sub-samples, wherein at least n-1sub-samples are labeled d) pulsing of the B cells of each sub-sample with single or multiple peptides e) pooling of the labeled and peptide-pulsed B cells with cells of said sample comprising said antigen-specific cells of step a) f) co-cultivation of the cells of step e) g) flow cytometry analysis of the B cells with regard to their cell number and/or CD83 expression loaded with single or multiple peptides of step d), wherein the potency of the antigen specific cells is determined by i) B cell numbers if extensive killing of target B cells occurs, ii) B cell numbers and ratio of CD83 expression on survived target B cells if moderate killing of target B cells occurs, or iii) ratio of CD83 expression on target B cells if no cytotoxic activity occurs.