Patent ID: 9650630
Date: 2017-05-16
CPC Classifications: C07K,C12N

Claim:
1. A nucleic acid construct for expression of a gene product of interest in a host cell comprising: (a) a nucleic acid fragment comprising a promoter sequence having at least 90% sequence identity to nucleotide residues 1236 to 2423 of SEQ ID NO:1 and 95% sequence identity to nucleotide residues 1782 to 1921 therein (b) an expression cassette comprising a promoter operably linked to a nucleotide sequence encoding the gene product of interest, and wherein the fragment is linked to the expression cassette in an orientation so that transcription from the promoter in the fragment is in the same direction as transcription from the promoter in the expression cassette, and (c) a nucleotide sequence encoding a selectable marker functional in a eukaryotic host cell; wherein, when the fragment is upstream and operably linked to the expression cassette having the nucleotide sequence of SEQ ID NO: 2 and in an orientation so that transcription from the promoter in the fragment is in the same direction as transcription from the promoter in SEQ ID NO: 2, the fragment produces at least 50% of number of colonies obtained with the same expression cassette when flanked with STARs 7 and 67 upstream of the expression cassette and STAR 7 downstream of the expression cassette, when tested under the conditions of Example 1.