Patent ID: 9631218
Date: 2017-04-25
CPC Classifications: C07K,C12P

Claim:
1. A conjugation method comprising: cloning a nucleic acid encoding a polypeptide, the nucleic acid comprises in series from 5′ to 3′ a coding sequence of a ligand with a coding sequence of a sortase recognition sequence, a coding sequence of a flexible linker, a coding sequence of sortase A, and a coding sequence of an affinity tag; expressing and purifying the polypeptide encoded by the nucleic acid; and adding calcium and a peptide or protein with an N-terminal glycine, wherein the addition of said calcium and said peptide or protein with an N-terminal glycine allows the sortase to catalyze ligand release and conjugation of the released ligand to said peptide or protein with an N-terminal glycine.