Patent ID: 9696303
Date: 2017-07-04
CPC Classifications: G01N

Claim:
1. A method of assaying a liquid sample for determining the presence and/or amount of a target nucleic acid analyte present or potentially present in the sample, the method comprising the steps of: (a) providing a capillary tube having immobilised therein a collection of first binding partners which comprise nucleic acid sequences capable of specifically binding to the analyte, said capillary tube further incorporating a collection of second binding partners which comprise nucleic acid sequences displaceably bound to a fraction of said first binding partners wherein 10-90% by mole of said first binding partners have said second binding partners bound thereto whereby there are free first binding partners immobilised within the capillary tube, said second binding partners having a label and being displaceable from the first binding partners by the analyte to be detected; (b) causing the liquid sample to flow from an upstream end of the capillary tube to a downstream end thereof; and (c) detecting for the presence of the label at the downstream end of the capillary tube.