Patent ID: 9539286
Date: 2017-01-10
CPC Classifications: A61K,A61L,C12N

Claim:
1. A method of making a bone graft comprising: obtaining bone including condyles, cortical bone, cancellous bone, and bone marrow from a donor; culturing osteogenic stem cells from the cancellous bone, bone marrow, or both by seeding cells onto a tissue culture substrate, allowing the cells to attach, washing the substrate to remove blood cells, and detaching osteogenic stem cells from the substrate; seeding the osteogenic stem cells on a scaffold consisting of osteoinductive demineralized bone obtained from the cortical bone and osteoconductive cortico-cancellous chips obtained from the condyles to form the bone graft, wherein the osteogenic stem cells, demineralized bone, and cortico-cancellous chips are derived from the same donor.