Patent ID: 9725727
Date: 2017-08-08
CPC Classifications: C12N,C12Y

Claim:
1. A variant strain of Trichoderma reesei derived from a parental strain, the variant strain comprising a deletion of a seb1 gene that causes cells of the variant strain to produce a reduced amount of functional Seb1 protein compared to cells of the parental strain, wherein the seb1 gene encodes a Seb1 protein comprising at least 88% sequence identity to the T. reesei Seb1 protein of SEQ ID NO: 5, wherein during aerobic fermentation in submerged culture the cells of the variant strain produce a cell broth that has a reduction in viscosity compared to the cells of the parental strain, wherein the reduction in cell broth viscosity of the variant strain (i) requires reduced agitation to maintain a preselected dissolved oxygen content compared to the cells of the parental strain, and/or (ii) maintains an increased dissolved oxygen content at a preselected amount of agitation, compared to the cells of the parental strain.