Patent ID: 9606111
Date: 2017-03-28
CPC Classifications: G01N,G06T,G16B

Claim:
1. A method of quantifying a total amount of protein in a complex target protein sample by detected haloalkylated tryptophan fluorescence, said method comprising: a) providing a complex target protein sample and a plurality of complex reference protein samples; b) exposing said complex target protein sample of step a) and said plurality of complex reference protein samples of step a) to UV light while said complex target protein sample and said complex reference protein samples are in contact with a haloalkane, thereby covalently reacting tryptophan residues of proteins in said complex target protein sample and said complex reference protein samples with said haloalkane, and c) detecting fluorescence emitted by said proteins in said complex target protein sample of step b) and said plurality of complex reference protein samples of step b), wherein said fluorescence is haloalkylated tryptophan fluorescence; d) providing a total amount of protein in said plurality of complex reference protein samples of step c), and quantifying an amount of haloalkylated tryptophan fluorescence detected from said plurality of complex reference protein samples of step c); e) quantifying an amount of haloalkylated tryptophan fluorescence detected from said complex target protein sample of step c); and f) quantifying said total amount of protein in said complex target protein sample based upon said total amounts of protein in said plurality of complex reference protein samples of step d) and said amounts of haloalkylated tryptophan fluorescence detected from said plurality of complex reference protein samples of step d) and said complex target protein sample of step e), wherein said complex target protein sample comprises 50 or more proteins and said plurality of complex reference protein samples comprise 50 or more proteins.