Patent ID: 9683214
Date: 2017-06-20
CPC Classifications: A61K,A61M,C12N

Claim:
1. A method for culturing bone marrow cells without use of an anticoagulant, comprising: (a) collecting from a subject a bone marrow fluid containing bone marrow cells in a liquid-tight state without use of an anticoagulant; (b) mixing the collected bone marrow fluid with a culture medium in a liquid-tight state at the site of collection of the bone marrow fluid without use of an anticoagulant to prepare an anticoagulant-free bone marrow cell suspension, wherein the culture medium is in an amount 1.5 times to 6 times the amount of the bone marrow fluid; and (c) directly culturing the bone marrow cells from step (b) in the anticoagulant-free bone marrow cell suspension.