Patent ID: 9631229
Date: 2017-04-25
CPC Classifications: C12Q

Claim:
1. A device for identification of nucleic acids by a method of real-time polymerase chain reaction comprising: a microchip; a microchip holder; a fluorescence detector, wherein the microchip includes a surface with at least one reaction zone, wherein the reaction zone is mechanically bound to the microchip holder and optically bound to the fluorescence detector via an emission channel spectral filtration system; at least one radiation source, wherein the at least one radiation source is optically bound to at least one excitation channel spectral filtration system and the microchip; and a control system, wherein the control system is electrically bound to a thermocycling block, wherein the thermocycling block is thermally bound to the microchip and is manufactured to heat, cool and maintain the microchip temperature, wherein the microchip contains a heat-conducting substrate thermally bound to the thermocycling block and made of aluminum with a thermal conductivity coefficient of more than 1 W/cm·K and a thermal diffusivity coefficient of more than 0.6 cm wherein each reaction zone is separated from the heat-conducting substrate by a layer of a passivating material comprised of silicon oxide and aluminum oxide covalently bound to the surface of the heat-conducting substrate, wherein one or several dried components of the polymerase chain reaction is placed over the layer of the passivating material, wherein an upper surface of the microchip has a peripheral barrier, wherein the peripheral barrier is configured to retain an assigned quantity of a liquid immiscible with water on an upper surface of the microchip, and wherein the ratio of the aggregate thermal mass of the microchip with introduced samples and the layer of the liquid immiscible with water, to thermal conductance of the microchip substrate does not exceed 0.04 seconds.