Patent ID: 9689023
Date: 2017-06-27
CPC Classifications: C12Q,G01N

Claim:
1. A method for molecular detection comprising: adding at least one molecular probe to a sample for detecting the presence of a protein target in said sample, said at least one molecular probe comprising an artificial nucleic acid aptamer having a binding affinity for the target; adding at least one reporter molecule to said sample, said at least one reporter molecule comprising an artificial nucleic acid comprising a circular nucleic acid with a conjugate region designed to be at least partially complementary to said artificial nucleic acid aptamer; adding at least one primer to said sample, said at least one primer being a separate molecule from said at least one molecular probe; and performing a quantitative or semi-quantitative amplification reaction on the at least one reporter molecule by primer extension of said primer; wherein said at least one molecular probe binds to said conjugate region of said at least one reporter molecule in the absence of the target and the binding impairs amplification of said at least one reporter molecule by said at least one molecular probe attaching to said at least one primer by a ligation reaction.