Patent ID: 9676826
Date: 2017-06-13
CPC Classifications: A61K,A61P,C07K,C12N,C12Y,Y02A

Claim:
1. A fusion protein comprising: (a) an antigen-presenting cell (APC)-binding domain or a CD91 receptor-binding domain, located at the N-terminus of the fusion protein; (b) a translocation peptide of 34-112 amino acid residues in length, comprising an amino acid sequence that is at least 90% identical to SEQ ID NO: 4, 2, 3, or 6, located at the C-terminus of the APC-binding domain or the CD91 receptor-binding domain; and (c) an antigen of a pathogen or a cancer cell; (d) an endoplasmic reticulum retention sequence, located at the C-terminus of the fusion protein; and (e) a nuclear export signal, located between the antigen and the endoplasmic reticulum retention sequence or between the translocation peptide and the antigen, the nuclear export signal comprising the amino acid sequence of SEQ ID NO: 13, in which the C-terminal amino acid of the SEQ ID NO: 13 is alanine; wherein: the pathogen is at least one selected from the group consisting of human papillomavirus (HPV), porcine reproductive and respiratory syndrome virus (PRRSV), human immunodeficiency virus-1 (HIV-1), dengue virus, hepatitis C virus (HCV), hepatitis B virus (HBV), porcine circovirus 2 (PCV2), classical swine fever virus (CSFV), foot-and-mouth disease virus (FMDV), Newcastle disease virus (NDV), transmissible gastroenteritis virus (TGEV), porcine epidemic diarrhea virus (PEDV), influenza virus, pseudorabies virus, parvovirus, swine vesicular disease virus (SVDV), poxvirus, rotavirus, and further wherein: the cancer cell is at least one selected from the group consisting of non-small cell lung cancer, breast carcinoma, melanoma, lymphomas, colon carcinoma, and hepatocellular carcinoma.