Patent ID: 9669082
Date: 2017-06-06
CPC Classifications: A61K

Claim:
1. A method of making a cancer vaccine for treating a cancer patient, and treating a cancer patient with the cancer vaccine, comprising: a) incubating proteinase K extracellularly with cancer cells in a culture dish; b) killing the cancer cells by using proteinase K to digest vital extracellular proteins, surface proteins and extracellular portion of membrane proteins of the cancer cells while maintaining the integrity of the plasma membrane of the cancer cells; c) collecting cancer cell spheres as said cancer vaccine when the cancer cells are completely detached from the culture dish, separated from each other, and rounded up to form cancer cell spheres, wherein said cancer cell spheres maintain the integrity of the plasma membrane of the cancer cells; and d) administering an effective amount of said cancer vaccine to a cancer patient to treat cancer.