Patent ID: 9675450
Date: 2017-06-13
CPC Classifications: A61F,A61L

Claim:
1. A method of constructing a pericardial heart valve replacement made from tissue of living autologous pericardium comprising: determining the size and shape of the patient's native pathological heart valve by real-time three-dimensional transesophageal echocardiography (TEE) or computer tomography (CT angiography) or by magnetic resonance imaging (MRI); constructing a living pericardial heart valve replacement for stented or stentless implantation from a single sheet of dynamically conditioned living autologous pericardium containing living pericardial interstitial cells (PICs) and a living extracellular matrix (ECM); tailoring the size and shape of the heart valve replacement so as to match the size and shape of the patient's native heart valve and aortic root; and dynamic conditioning and hemodynamic testing for conditioning and modification of the living autologous pericardium a device for a period of approximately 7 to 21 days in a device, the device comprising a hydrodynamic circuit of an artificial heart whereby hemodynamic loading of the living pericardial heart valve replacement induces pericardial interstitial cell (PIC) activation and phenotypic differentiation resulting in the production and remodeling of pericardial extracellular matrix (ECM) with an increase in the content of collagen and elastic fibers and glycosaminoglycans, thereby improving the mechanobiological and hemodynamic properties of the living pericardial heart valve replacement.