Patent ID: 9701756
Date: 2017-07-11
CPC Classifications: C07K

Claim:
1. A humanized antibody or fragment thereof specific for the antigen CD45R0, wherein said antibody or fragment thereof comprises a humanized heavy chain variable domain comprising a CDR1 region of SEQ ID NO:1, a CDR2 region of SEQ ID NO:2, and a CDR3 region of SEQ ID NO:3, and a humanized light chain variable domain comprising a CDR1 region of SEQ ID NO:4, a CDR2 region of SEQ ID NO:5, and a CDR3 region of SEQ ID NO:6; wherein said humanized heavy chain variable domain has artificial framework regions FR1, FR2, FR3, and FR4, and wherein said humanized light chain variable domain has artificial framework regions FR1, FR2, FR3, and FR4; wherein said artificial framework regions FR1, FR2, FR3, and FR4 of said humanized heavy chain variable domain are amino acid sequences having an identity of at least 70% to the artificial heavy chain variable sequence framework regions FR1, FR2, FR3, and FR4 of SEQ ID NO:19, and wherein said artificial framework regions FR1, FR2, FR3, and FR4 of said humanized light chain variable domain are amino acid sequences having an identity of at least 70% to the light chain variable sequence framework regions FR1, FR2, FR3, and FR4 of SEQ ID NO:20; and wherein said artificial framework regions FR1, FR2, FR3, and FR4 of said humanized heavy and light chain variable domains are amino acid sequences comprising within the heavy chain variable domain amino acid F at position 25, V at position 39, G at position 40, I at position 42, L at position 53, T at position 54, H at position 55, Y at position 66, K at position 80, S at position 82, and V at position 87; and within the light chain variable domain amino acid S at position 25, L at position 39, H at position 40, N at position 66, R at position 67, and F at position 68 according to the IMGT® nomenclature; and wherein said antibodies or fragments thereof bind specifically to CD45R0 with a relative affinity of at least 20% for the CD45R0 antigen when compared to the murine CD45R0 antibody comprising the heavy chain variable domain set forth SEQ ID NO:13 and the light chain variable domain set forth in SEQ ID NO:14.