Patent ID: 9593364
Date: 2017-03-14
CPC Classifications: C12Q,G01N

Claim:
1. A method for detection of a target in a sample, the method comprising the steps of; providing an assay mixture comprising: (i) a first probe comprising a first antibody recognizing a first epitope of the target, the first antibody conjugated to an RNA oligonucleotide; (ii) a second probe comprising a second antibody recognizing a second epitope of the target, the second antibody conjugated to a DNA oligonucleotide; (iii) a reverse primer, wherein the reverse primer comprises a first region complementary to the RNA oligonucleotide, and a second region complementary to said DNA oligonucleotide; and (iv) a reverse transcriptase, wherein said reverse transcriptase creates a DNA transcription product from the RNA oligonucleotide using said reverse primer only if said RNA oligonucleotide and said DNA oligonucleotide are in close proximity; adding the sample to the assay mixture to create a reaction mixture; incubating the reaction mixture for a predetermined period of time under conditions suitable for reverse transcription by said reverse transcriptase; and analyzing said reaction mixture for the presence of said DNA transcription product of the RNA oligonucleotide; wherein when the target is present in the sample, and the first antibody is interacting with the first epitope, and the second antibody is interacting with the second epitope, the first region of the reverse primer binds the RNA oligonucleotide and the second region of the reverse primer binds the DNA oligonucleotide to bring the RNA oligonucleotide and the DNA oligonucleotide in close proximity; wherein the presence of said DNA transcription product indicates the presence of the target in said sample.