Patent ID: 9540702
Date: 2017-01-10
CPC Classifications: C12N,C12Q,C12Y

Claim:
1. A method for stabilizing an enzyme, which comprises: storing the enzyme in a presence of a stabilized coenzyme, or a salt form thereof, or a reduced form thereof for a period of storage of at least 2 weeks at a temperature of at least 20° C., wherein the enzyme is selected from the group consisting of an alcohol dehydrogenase (EC 1.1.1.2), a glucose-6-phosphate dehydrogenase (EC 1.1.1.49) and a diaphorase (EC 1.6.99.2), wherein the stabilized coenzyme is a compound of general formula (II): in which A=adenine or an analogue thereof; T=in each case independently denotes O or S; U=in each case independently denotes OH, SH, BH 3 − , or BCNH 2 − ; V=in each case independently denotes OH or a phosphate group, or two groups which form a cyclic phosphate group; W=COOR, CON(R) 2 , COR, or CSN(R) 2 in which R in each case independently denotes H or a C 1 -C 2 alkyl; X 1 , X 2 =in each case independently denote O, CH 2 , CHCH 3 , C(CH 3 ) 2 , NH, or NCH 3 ; Y=NH, S, O, or CH 2 ; Z=a linear residue with 4-6 C atoms or a saturated or an unsaturated carbocyclic or heterocyclic 5-membered ring of general formula (III): in which a single or double bond may be present between R 5′ and R 5″ , where R 4 =in each case independently denotes H, F, Cl, or CH 3 ; R 5 =CR 4 2 ; R 5′ =O, S, NH, NC 1 -C 2 -alkyl, CR 4 , CHOH, or CHOCH 3 , and R 5″ =CR 4 2 , CHOH, or CHOCH 3 if there is a single bond between R 5′ and R 5″ ; R 5′ =R 5″ =CR 4 if there is a double bond between R 5′ and R 5″ ; and R 6 , R 6′ =in each case independently denote CH or CCH 3 ; wherein the Z and the pyridine residue are not linked by a glycosidic bond, and wherein enzyme activity decreases by less than 50% after the period of storage of at least 2 weeks at a temperature of at least 20° C. when compared to an initial value of enzyme activity.