Patent ID: 9644233
Date: 2017-05-09
CPC Classifications: C12Q

Claim:
1. A method of amplifying a nucleic acid, comprising initially denaturing a target sequence containing a mutation sequence, as well as a forward primer and a reverse primer respectively having a target recognizing sequence and complementary sequences on both 3′ end and 5′ end thereof to form a single-loop structure having a blunt end before denaturing, wherein the forward primer further has a mutation recognizing sequence for recognizing the mutation sequence; annealing the forward primer and the reverse primer with the target sequence; extending the forward primer and the reverse primer along the target sequence; denaturing the target sequence, as well as the extended forward and reverse primers, wherein the sequence extended from the forward primer is an object sequence to be amplified by a subsequent polymerase chain reaction (PCR) and wherein the method excludes use of DNA helicase.