Patent ID: 9541549
Date: 2017-01-10
CPC Classifications: G01N

Claim:
1. A method for the determination of the mid-regional partial peptide of proadrenomedullin (mid-proAM) in a patient employing at least two antibodies capable of binding within the region of amino acids 60-92 of preproadrenomedullin (SEQ ID NO:1), wherein the method comprises conducting a two-sided immunoassay by contacting plasma of the patient with two antibodies that specifically bind to different partial sequences of mid-proAM (SEQ ID NO: 3), so as to form sandwich complexes comprising the two antibodies and mid-proAM; and detecting the sandwich complexes, thereby determining mid-proAM; wherein one of the antibodies is obtained by immunization of an animal with an antigen which contains a synthetic peptide sequence which consists of SEQ ID NO: 4, and the other of the antibodies is obtained by immunization with an antigen which contains a synthetic peptide sequence which consists of SEQ ID NO: 5.