Patent ID: 9689032
Date: 2017-06-27
CPC Classifications: C12Q

Claim:
1. A method for sequencing a target nucleic acid, comprising: (a) sequencing one or more bases of a target nucleic acid by extending a first sequencing primer hybridized to said target nucleic acid to generate a first primer extension product, thereby obtaining a first sequence read; (b) releasing said first primer extension product from said target nucleic acid; (c) hybridizing a second sequencing primer to said target nucleic acid; (d) generating a second primer extension product by extending said second sequencing primer through limited extension without the use of labeled nucleotides; (e) sequencing one or more bases of said target nucleic acid by further extending said second primer extension product to generate a third primer extension product, thereby obtaining a second sequence read; and (f) determining a sequence of said target nucleic acid by assembling said first sequence read and said second sequence read.