Patent ID: 9714422
Date: 2017-07-25
CPC Classifications: A61P,C12N,C12Q

Claim:
1. A method of inducing processing of a partially processed mRNA transcript to facilitate removal of an entire retained intron to produce a fully processed mRNA transcript that encodes a functional form of a protein, the method comprising: (a) contacting an isolated polynucleic acid polymer to a target cell of a subject, wherein the polynucleic acid polymer is from about 10 to about 50 nucleotides in length; (b) hybridizing the isolated polynucleic acid polymer to a wild-type target sequence of the partially processed mRNA transcript, wherein the polynucleic acid polymer comprises a sequence that is complementary to at least 10 contiguous bases of the wild-type target sequence, wherein the partially processed mRNA transcript is capable of encoding the functional form of a protein and comprises at least one entire retained intron, optionally interfering with one or more conformational transitions of canonical and noncanonical RNA structures or interacting with trans-acting factors that bind to an intron; (c) removing the at least one entire retained intron from the partially processed mRNA transcript to produce the fully processed mRNA transcript that encodes the functional form of a protein; and (d) translating the functional form of a protein from the fully processed mRNA transcript.