Patent ID: 9644241
Date: 2017-05-09
CPC Classifications: C12Q

Claim:
1. A method of treating a patient that has pancreatic ductal adenocarcinoma (PDAC) comprising: a) measuring by reverse-transcriptase polymerase chain reaction (RT-PCR) from a fine needle aspirate (FNA) pancreatic sample from the patient the level of expression of up to 11 different miRNAs that include at least miR-135b and at least 4 of the following diff pair miRNAs: miR148a, miR-130b, miR-196a, miR-24, miR-375, and miR-96, wherein at least one of the miRNAs is a biomarker miRNA and one is a comparative miRNA and miR-217 is not one of the miRNAs; b) determining a diff pair value for diff pairs: miR135b/miR-24, miR130b/miR-135b; miR-135b/miR-148a; miR-148a/miR-196a; miR-135b/miR-96; miR-375/miR-135b and optionally miR-130b/miR-24 and, c) analyzing the diff pair values using a computer performing linear discriminate analysis, wherein the linear discriminate identifies the patient as having PDAC; and d) treating the identified patient with chemotherapy, radiotherapy, surgery, or immunotherapy to treat the PDAC.