Patent ID: 9658216
Date: 2017-05-23
CPC Classifications: C12N,G01N

Claim:
1. A method of selecting mammalian retinal pigmented epithelial (RPE) cells suitable for cell therapy from a mixed population of cells which comprise non-pigmented cells, comprising: (a) ex-vivo differentiating mammalian pluripotent stem cells towards an RPE lineage to generate a mixed population of cells which comprise pigmented and non-pigmented cells; (b) using a fluorescence activated cell sorter (FACS) with a 780/60 nm FACS filter to identify and select cells from said mixed population of cells for at least one of the following parameters: (c) culturing the cells from step (b) in a medium comprising nicotinamide, thereby selecting mammalian RPE cells suitable for cell therapy.