Patent ID: 9534233
Date: 2017-01-03
CPC Classifications: C12N

Claim:
1. A nucleic acid construct comprising (i) an expression unit for the expression of E1 B, wherein the expression unit comprises a promoter, a nucleotide sequence coding for E1 B, and a 3′ UTR, wherein the promoter is operatively linked to the nucleotide sequence coding for E1 B, wherein the 3′ UTR comprises 30 or fewer than 30 Exonic Splicing Enhancer elements (ESEs), and wherein the 3′ UTR is a non-viral 3′ UTR, and (ii) an expression unit for the expression of E1A, wherein the expression unit comprises a promoter, a nucleotide sequence coding for E1A, and a 3′ UTR, wherein the promoter is operatively linked to the nucleotide sequence coding for E1A, wherein the expression unit for the expression of E1A and the expression unit for the expression of EIB form a combined expression unit, wherein the combined expression unit comprises the nucleotide sequence of SEQ ID NO: 9 or SEQ ID NO: 15.