Patent ID: 9650621
Date: 2017-05-16
CPC Classifications: C07K,C12N,C12P,C12Q,C12Y,G01N

Claim:
1. A mutant Releasing Factor 1 protein (RF1), wherein the mutant RF1 comprises an amino acid sequence that has at least 95% sequence identity to the amino acid sequence of SEQ ID NO: 1 and has activity to recognize a stop codon in an mRNA sequence and terminate translation, wherein the mutant RF1 protein comprises arginine at each of the amino acids corresponding to residues 294 and 295 of SEQ ID NO: 1, wherein asparagine at the amino acid corresponding to residue 296 of SEQ ID NO: 1 is replaced with arginine or lysine in the amino acid sequence of the mutant RF1, and wherein the sequence of amino acids of the mutant RF1 protein corresponding to residues 294, 295, and 296 of SEQ ID NO: 1 is a cleavage site for a wild-type Outer Membrane Protein T1 (OmpT1).