Patent ID: 9557340
Date: 2017-01-31
CPC Classifications: G01N

Claim:
1. A method for detecting the presence of pegylated Anabaena variabilis phenylalanine ammonia-lyase (AvPAL-PEG)-specific antibodies in a sample, said method comprising: (a) contacting the sample with recombinant AvPAL-PEG enzyme, wherein the recombinant AvPAL-PEG enzyme is immobilized on a solid support via a PEG-specific antibody; (b) optionally removing unbound sample; (c) adding a phenylalanine substrate; and (d) detecting the presence of enzymatic activity between the immobilized recombinant AvPAL-PEG enzyme and phenylalanine substrate; wherein a reduction of enzymatic activity as compared to a reference sample having no neutralizing AvPAL-PEG-specific antibodies indicates the presence of neutralizing AvPAL-PEG-specific antibodies in the sample.