Patent ID: 9683268
Date: 2017-06-20
CPC Classifications: A61K,C12N,C12Q

Claim:
1. A method of detecting acquired immune deficiency syndrome (AIDS) and/or AIDS progression in a subject infected with HIV or SIV, said method comprising: a) synthesizing cDNA from RNA comprising a biological sample obtained from a subject; b) synthesizing cDNA from RNA comprising a control sample; c) detecting in each sample the quantity of WUHARV Adenovirus 1 by a PCR assay using primer pairs selected from the group consisting of GGCAATCATGATGGACACCTT(SEQ ID: 332)and TTAATCACCACCGCAACGC (SEQ ID NO:3:33), CAATGGAACATTAATCCCACG (SEQ ID NO: 334) and CCTGCCAACACTCCCATATTT (SEQ ID NO: 335), and AGAGCTATCACACAGCGTTCA (SEQ ID NO: 366) ACCGAGTGGTGGAGGAGAA (SEQ ID NO: 337), wherein the PCR assay is selected from the group consisting of a real time PCR assay and a nested PCR assay; d) determining the magnitude of difference between the quantity of WUHARV Adenovirus 1 in said biological sample relative to the quantity of WUHARV Adenovirus 1 in said control sample; and e) detecting CD4 T cell levels in the subject and in a control, wherein a statistically significant increase in the quantity of WUHARV Adenovirus 1, and a decrease in CD4 T cell levels in said subject, relative to the control, indicates AIDS and/or AIDS progression in said subject.