Patent ID: 9676836
Date: 2017-06-13
CPC Classifications: C07K,C12N

Claim:
1. A cDNA nucleic acid operatively linked to a heterologous promoter sequence, said nucleic acid comprising: a) a sequence that encodes a peptide with at least 95% homology to an amino acid sequence of SEQ ID NO: 3; wherein the nucleic acid encodes a red-shifted channelrhodopsin or b) a sequence that encodes a peptide comprising 225 contiguous amino acids of SEQ ID NO: 3; wherein the nucleic acid encodes a red-shifted channelrhodopsin or c) a sequence that hybridizes under highly stringent conditions to a nucleotide sequence that encodes amino acids of SEQ ID NO: 3, or a complement thereof, wherein the cDNA nucleic acid encodes helices b and c of a red-shifted channelrhodopsin helix domain, and wherein said highly stringent conditions comprise hybridizing to a filter-bound DNA in 0.5 M NaHPO4, 7% sodium dodecyl sulfate (SDS), and 1 mM EDTA at 65° C.; and washing in 0.1×SSC/0.1% SDS at 68° C.