Patent ID: 9732342
Date: 2017-08-15
CPC Classifications: C12N

Claim:
1. A double-stranded segmented miRNA mimetic molecule represented by Formula IV: wherein (a) the molecule comprises a passenger strand and a guide strand where each line and its adjacent “N” represent a contiguous stretch of nucleotides, each of “X1,” “X2,” “X3” and “X4” represents the number of nucleotides in each stretch, “G/N” represents a discontinuity in the guide strand, “P/N” represents a discontinuity in the passenger strand, “Y1” represents a number of nucleotide positions in the passenger strand discontinuity, “Y2” represents a number of nucleotide positions in the guide strand discontinuity, and each group of dashed lines “-----” and its adjacent “(W)” represents a terminal overhang that is optionally present, and each of “Z1” and “Z2” represents the number of overhanging nucleotides; (b) X1 and X2 are independently integers from 2 to 24, Y1 is an integer from 0 to 6, provided that the sum of X1, X2 and Y1 is an integer from 16 to 26, X3 is an integer from 2 to 20, X4 is an integer from 6 to 24, Y2 is an integer from 0 to 6, provided that the sum of X3, X4 and Y2 is an integer from 16 to 26; Z1 and Z2 are independently integers from 0 to 4; (c) N (d) N (e) N wherein each of G/N and P/N independently comprises a nick, gap, non-nucleotide substitution, or non-nucleotide insertion.