Patent ID: 9695454
Date: 2017-07-04
CPC Classifications: C07K,C12N,C12P

Claim:
1. A Trichoderma cell for producing glycoproteins with mammalian-like fucosylated N-glycans, comprising: 1) at least a deletion or disruption mutation in an endogenous protease encoding gene, which mutation reduces the expressed endogenous protease compared to a parental filamentous fungal cell which does not have the mutation, 2) an exogenous polynucleotide encoding a polypeptide having α1,6 fucosyltransferase activity, and 3) one or more exogenous polynucleotides encoding a polypeptide having GDP-fucose synthesis activity, wherein GDP-fucose synthesis activity comprises: a) a polynucleotide or a functional variant polynucleotide encoding a polypeptide having GDP-mannose-dehydratase (GMD) activity and, b) a polynucleotide or a functional variant polynucleotide encoding a polypeptide having both GDP-keto-deoxy-mannose-epimerase and GDP-keto-deoxy-galactose-reductase (FX) activities.