Patent ID: 9632093
Date: 2017-04-25
CPC Classifications: C12Q,C12Y,G01N

Claim:
1. A method for characterizing a biological sample comprising: (a) obtaining a biological sample from a human individual; (b) measuring a K-ras mutation score within the obtained biological sample through exposing a portion of the biological sample to one or more K-ras specific primers and determining the K-ras mutation score by performing a digital melt curve analysis or quantitative allele-specific PCR, wherein the K-ras specific primers comprise a nucleic acid sequence selected from the group consisting of SEQ ID NOs: 2, 3, 4, 5, 6, 7 and 8; (c) measuring a methylation level of a CpG site for BMP3 in a portion of the obtained biological sample through (d) comparing the K-ras mutation score and the BMP3 methylation level to a corresponding set of control samples without colorectal cancer; and (e) determining that the individual has colorectal cancer when 1) the measured K-ras mutation score is higher than in the control sample, and 2) the measured BMP3 methylation level is higher than in the control sample.