Patent ID: 9719105
Date: 2017-08-01
CPC Classifications: A61K,A61P,C12N

Claim:
1. A method for producing a vaccinia virus comprising: (a) infecting HeLa cells adhered to a surface with a vaccinia virus by contacting the HeLa cells with vaccinia virus at a multiplicity of infection (m.o.i.) of between 0.01 to 0.05 plaque forming units (pfu)/cell; (b) culturing the infected cells in an infection medium maintained at a pH between 7.2 and 7.4 for a period of 40-80 hours at a temperature between 36° C. and 37.5° C.; and (c) harvesting vaccinia virus from the culture, whereby at least 50 pfu of vaccinia virus per HeLa cell is produced calculated as: wherein the HeLa cells are adhered to a plurality of plastic roller bottle containers comprising a cumulative culture area of at least 168,000 cm 2 .