Patent ID: 9718871
Date: 2017-08-01
CPC Classifications: C07K,C12N

Claim:
1. A method of generating a variant of an antibody, an antigen-binding domain of the antibody or a T-cell receptor (TCR), wherein the variant binds to an antigen with increased antigen-binding affinity, the method comprising the steps of: (i) providing a polynucleotide comprising: (ii) introducing the polynucleotide into a recombination-competent host cell, (iii) culturing the host cell in vitro under conditions allowing recombination and expression of the nucleic acid sequence, and (iv) identifying variants of the antibody, antigen-binding domain, or TCR with increased antigen-binding affinity relative to affinity of the antibody, the antigen-binding domain of the antibody, or the TCR to the antigen.