Patent ID: 9598476
Date: 2017-03-21
CPC Classifications: A61K,A61P,C07K,G01N

Claim:
1. A nucleic acid molecule comprising a nucleotide sequence encoding a mutein of human tear lipocalin, wherein the mutein comprises: (a) a mutated amino acid residue at any one or more of the sequence positions 26-34, 56-58, 80, 83, 104-106 and 108 of the linear polypeptide sequence of the mature human tear lipocalin, wherein the mutein comprises at least one of the following amino acid substitutions in comparison to mature human tear lipocalin: (i) Arg 26→Ser, Phe, Trp, His or Thr and (ii) Glu 34→Asn, Thr, Arg or Gly; and wherein the mutein further comprises the following amino acid substitutions: Pro 29→Gly, Asp, Asn, Ile, Leu or Met and Lys 108→Gln, Ala, Trp, Tyr, Arg, Asp, Asn, Ser, Glu or Thr and (b) a mutated amino acid residue at any one or more of the sequence positions 61, 101, 111, 114 and 153 of the linear polypeptide sequence of the mature human tear lipocalin, wherein the mutein encoded by the nucleotide sequence has at least 75% identity to the sequence of mature human tear lipocalin set forth in SEQ ID NO: 1, and wherein the mutein binds specifically to proprotein convertase subtilisin/kexin type 9 (PCSK9).