Patent ID: 9745587
Date: 2017-08-29
CPC Classifications: C12N

Claim:
1. A recombinant nucleic acid comprising a nucleotide sequence comprising (a) a plurality of constructs comprising a first pair of recombinase recognition sequences which is recognized by a first recombinase, wherein the first pair of recombinase recognition sequences flank a first terminator, and a second pair of recombinase recognition sequences which is recognized by a second recombinase, wherein the second pair of recombinase recognition sequences flank a second terminator, (b) a promoter upstream of the plurality of constructs capable of transcribing the plurality of constructs, wherein each construct independently comprises a nucleotide sequence of interest flanked by a pair of recombinase recognition sequences, wherein each pair of recombinase recognition sequences is recognized by a recombinase that does not cross-react with the recombinase recognition sequences of another construct, and the two recombinase recognition sequences of each pair are oriented in the same direction to each other, and (c) a target gene downstream of the plurality of constructs; wherein the first recombinase and the second recombinase are (i) Escherichia coli Cre and Dre, or (ii) Dre and Saccharomyces cerevisiae FLP.