Patent ID: 9675713
Date: 2017-06-13
CPC Classifications: A61K

Claim:
1. A composition comprising a nanoparticle probe comprising a nanoparticle core comprising a detectable signal-emitting superconducting polymer, a shell attached to the superconducting polymer core by hydrophobic tails and having surface exposed reactive groups, wherein said reactive groups form covalent bonds with a quencher moiety, wherein the nanoparticle probe is an NH 2 -functionalized conjugated polymer nanoparticle (NH 2 -CPN) comprising a core nanoparticle comprising poly[9,9′-dihexyl-2,7-bis(1-cyanovinylene)fluorenylene-alt-co-2,5-bis(N,N′-diphenylamino)-1,4-phenylene] (PCFDP) and the hydrophobic lipid tails of 1,2-Dimyristoyl-sn-glycero-3-phosphoethanolamine-N-[methoxy(polyethylene glycol)-2000] (DSPE-PEG) and 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[amino(polyethylene glycol)-2000] (DSPE-PEG-NH 2 ), whereby the N-[methoxy(poly ethylene glycol)-2000] domain of the DSPE-PEG, and the N-[amino(polyethylene glycol)-2000] domain of the DSPE-PEG-NH 2 comprise the shell substantially surrounding the core nanoparticle; and the quencher is the dye (2-[2-[2-Chloro-3-[2-(1,3-dihydro-1,3,3-trimethyl-2H-indol-2-ylidene)-ethylidene]-1-cyclohexen-1-yl]-ethenyl]-1,3,3-trimethyl-3H-indolium chloride conjugated to the shell.