Patent ID: 9677129
Date: 2017-06-13
CPC Classifications: A01K,C12N,C12Q

Claim:
1. A method for detecting the replacement of an endogenous allele with a modified allele by homologous recombination in a mouse embryonic stem (ES) cell, the method comprising: (a) exposing the DNA of the mouse ES cell to a first probe and a second probe, wherein the first probe binds within the endogenous allele but not within the modified allele, and the second probe binds within a reference gene but not within the endogenous allele and not within the modified allele, wherein both probes generate a detectable signal upon binding, and wherein the reference gene is of known copy number; (b) detecting the signal from the binding of the first probe and the signal from the binding of the second probe; and (c) comparing the signal from the binding of the second probe to the signal from the binding of the first probe, and determining from the comparison a copy number of the endogenous allele.