Patent ID: 9542526
Date: 2017-01-10
CPC Classifications: C12Q,G16B

Claim:
1. A computerized method for correcting a measured melting temperature of a nucleic acid in a sample, wherein the melting temperature refers to the dissociation of double stranded DNA into single stranded DNA, wherein a first reference sample comprising a nucleic acid, and a second nucleic acid containing sample are utilized, comprising: a) determining a ratio of the concentration of the nucleic acid in the first reference sample to the concentration of the nucleic acid in the second nucleic acid containing sample, wherein the first and second samples contain identical nucleic acids in different concentrations; b) using an imaging system and a temperature control system in communication with a computer to measure the melting temperature of the nucleic acid in the second nucleic acid containing sample (T c) determining a temperature compensation value from said ratio and determining the change of enthalpy (ΔH T d) correcting the measured melting temperature of the nucleic acid in the second nucleic acid containing sample with the temperature compensation, wherein the correcting the measured melting temperature is performed by the computer based upon the formula T