Patent ID: 9637717
Date: 2017-05-02
CPC Classifications: C12M,C12N,Y10T

Claim:
1. A bioreactor system for a cell culture procedure, comprising: a bioreactor vessel configured to contain a fluid cell culture media, cultured cell material and micro-carriers of a predetermined diameter, the vessel being formed of a medical grade plastic having a plurality of outer walls; a fluid agitator configured to move within the vessel rotatable about a horizontal axis so as to create a fluid flow of the cell culture media and micro-carriers within the vessel, including an area of fluid flow tangent to a first one of the outer walls located below the fluid agitator; and a port open to the first outer wall configured for removing fluid cell culture media from the vessel and comprising a retention screen formed by pores in and opening through the first outer wall, wherein the retention screen is oriented parallel to the tangent flow and includes pores sized to retain the micro-carriers in the vessel while fluid cell culture media is removed from the vessel via the media removal port, wherein the tangent flow prevents the micro-carriers from settling on and clogging the pores.