Patent ID: 9579350
Date: 2017-02-28
CPC Classifications: A61K,A61L,A61P

Claim:
1. A method for preparing a composition of sterile, de-cellularized human amniotic fluid (D-HAF) comprising: (a) collecting amniotic fluid under sterile conditions from a subject to produce a sample of raw amniotic fluid; (b) de-cellularizing the raw amniotic fluid to remove only cells and/or particulate matter by a series of centrifugation and filtration steps to produce a de-cellularized amniotic fluid, wherein the quantity of the proteins in the de-cellularized amniotic fluid is between 50% to 100% of the raw amniotic fluid; and (c) placing the de-cellularized amniotic fluid in a sterile vessel at a temperature from about 1° C. to about 20° C. for one or more days, weeks, months, or up to a year, or more than a year, wherein the de-cellularized amniotic fluid is not concentrated, diluted, fractionated, heat- or alcohol-treated relative to the raw fluid.