Patent ID: 9677109
Date: 2017-06-13
CPC Classifications: B01L,C12M,C12Q,G16B

Claim:
1. A method comprising: introducing a patient sample comprising a viable microorganism into a microfluidic flow cell having a detection surface; retaining the microorganism on the detection surface of the microfluidic flow cell, thereby producing a retained microorganism; introducing a gel medium into the microfluidic flow cell, wherein the gel medium is in contact with the retained microorganism following introduction into the microfluidic flow cell; immobilizing via the gel medium the retained microorganism in the microfluidic flow cell at the same location where the microorganism is retained, to produce an immobilized microorganism, wherein offspring of the immobilized microorganism remain over time at a location with the immobilized microorganism; and incubating the immobilized microorganism for a first time period, wherein the first time period comprises a first time and a second time.