Patent ID: 9631172
Date: 2017-04-25
CPC Classifications: A61L,B03C,C12N,C25D,G01N

Claim:
1. A method for manufacturing a three-dimensional (3D) biomimetic scaffold comprising: a) contacting a mobile phase with an immobile phase through an electrical insulating material containing the mobile phase, said electrical insulating material selected from the group consisting of: b) subjecting said mobile phase and said immobile phase in the presence of the electrical insulating material to a direct current (DC) electric field created inside a chamber and applied in closed circuit by a pair of electrodes in vertical or horizontal configuration, said chamber containing the mobile phase, the immobile phase and the electrical insulating material covered by a buffer; and c) patterning the immobile phase with the components of the mobile phase, in which said components move by forming patterns within the immobile phase and are not separated by their molecular weight and in which the location of the components of the mobile phase is controlled as desired by modulating the electric field and the time; wherein the mobile phase is an aqueous or organic medium comprising at least a charged compound and/or a charged macromolecular entity; and wherein the immobile phase is any previously polymerized hydrogel.