Patent ID: 9669086
Date: 2017-06-06
CPC Classifications: A61K,A61P,C12N

Claim:
1. A method for eliciting a protective immune response in a pig against Mycoplasma hyopneumoniae ( M. hyo ) comprising administering to said pig a single dose of an immunogenic composition which comprises porcine circovirus type 2 antigen and M. hyo antigen, wherein the amount of the M. hyo antigen per a single dose is equivalent to 40-fold the amount of a determined relative potency value in mice, wherein the relative potency value in mice for M. hyo is determined by the steps: (a) making serial dilutions of a composition containing an unknown amount (b) dividing a plurality of mice into groups to form a number of mice groups, wherein the number of mice groups is the same as the number of serial dilution groups and each mice group contains a plurality of mice; (c) administering a single dose of each serial dilution group containing said (d) taking a biological sample from each mouse in each mice group for each serial dilution group after a period of 21 days; (e) detecting (f) determining the relative potency value of the (g) calculating the amount of (h) administering to said pig an immunogenic composition which comprises porcine circovirus type 2 antigen and