Patent ID: 9534217
Date: 2017-01-03
CPC Classifications: C12N,C40B

Claim:
1. A method of creating a library of artificial bacterial promoters comprising: a) obtaining an insertion DNA cassette, wherein the DNA cassette comprises a sense strand having a first end (5′) and a second end (3′), an antisense strand having a first end (5′) and a second end (3′), wherein the DNA cassette comprises a first recombinase site, a second recombinase site and a marker gene located between the first and the second recombinase sites; b) obtaining a first oligonucleotide which comprises in sequential order from the 5′ end, c) obtaining a second oligonucleotide which comprises in sequential order from the 3′ end, d) mixing the first oligonucleotide, the second oligonucleotide and the DNA cassette, and e) performing a polymerase chain reaction (PCR) on the mixture of (d) to obtain a library of double stranded amplified products comprising artificial bacterial promoters.