Patent ID: 9708389
Date: 2017-07-18
CPC Classifications: A61K,C07K,C12P

Claim:
1. A method for obtaining a mixture containing two or more proteins by using a single recombinant cell clone, wherein the proteins are dimers formed by polymerization between two monomer chains, and the two or more proteins contain the same domain, wherein the method comprises the step of replacing one or more amino acid residues of the two monomer chains in the same domain of one or more proteins with opposite-charged residues, so that the monomer chains from different proteins are unfavorable to forming a heterodimer due to the repulsive interaction between like charges, while the monomer chain from the same protein is more favorable to forming a homodimer due to the attractive interaction between opposite charges; wherein the same domain is a CH3 domain of an antibody or a Fc region of an antibody; and wherein an opposite-charged residue is introduced to replace: wherein: the positions of the residues are determined according to the EU numbering index of the KABAT system.