Patent ID: 9618503
Date: 2017-04-11
CPC Classifications: G01N

Claim:
1. A method for identifying sperm in a cellular population containing reactive oxygen species, comprising sequentially a) through d): a) contacting a cellular population of sperm with a thickening agent containing an indicator compound, said cellular population of sperm moving with reduced motility in said thickening agent; b) maintaining said mixture obtained in a) in conditions to avoid gelling over a period of time to convert of said indicator compound to a detectable compound in said sperm containing said reactive oxygen species; c) placing the mixture obtained in b) on a solid support in conditions suitable to gel said mixture to immobilize said cellular population of sperm; and d) identifying said sperm in said cellular population of sperm in which said detectable compound appears, said detectable compound appearing in said sperm indicative of said sperm containing said reactive oxygen species.