Patent ID: 9689015
Date: 2017-06-27
CPC Classifications: C12N,C12P,C12Y,Y02P

Claim:
1. A method for demannosylating phosphorylated N-glycans on a glycoprotein, said method comprising contacting in vitro a recombinant glycoprotein comprising a terminal alpha-1,2 mannose linkage in which the underlying mannose is phosphorylated with a mannosidase that hydrolyzes a terminal alpha-1,2 mannose linkage when the underlying mannose is phosphorylated, wherein the contacting results in hydrolysis of the terminal alpha-1,2 mannose linkage, to produce a demannosylated glycoprotein, wherein the mannosidase is: (a) a wild-type glycoside hydrolase (GH)92 family polypeptide or a wild-type GH47 family polypeptide; (b) a deletion variant that is the polypeptide of (a) but lacks up to twenty amino acid segments, each segment consisting of two amino acids; (c) a deletion variant that is the polypeptide of (a) or the variant of (b) but lacks up to twenty single, non-contiguous amino acids of the polypeptide of (a) or the variant of (b); or (d) a substitution variant that is the polypeptide of (a), the variant of (b), or the variant of (c), but with no more than 10 conservative substitutions.