Patent ID: 9745570
Date: 2017-08-29
CPC Classifications: C12N,C12Q

Claim:
1. A method of generating a rRNA-depleted sample from an initial sample comprising: a) providing: b) contacting said initial sample with said composition comprising affinity-tagged antisense rRNA molecules under conditions such that at least some of said affinity-tagged antisense rRNA molecules and at least some of said rRNA molecules form double-stranded rRNA hybrids, thereby generating a treated sample; c) contacting said treated sample with said binding matrix under conditions wherein the ratio of said affinity-tag-binding molecules to said affinity-tags present in the antisense rRNA molecules used in step b) is at least 8 to 1, such that at least a portion of said double-stranded rRNA hybrids bind to said binding matrix and d) removing said matrix-bound double-stranded rRNA hybrids from said treated sample, thereby generating an rRNA-depleted sample, wherein said rRNA-depleted sample comprises at least a portion of said non-rRNA RNA molecules present in said initial sample and is substantially depleted of rRNA molecules that exhibit sequences exhibited by said at least one rRNA molecule.