Patent ID: 9593316
Date: 2017-03-14
CPC Classifications: C12N,C12Q,C12Y,G01N

Claim:
1. An isolated polynucleotide comprising a first sequence encoding a mutant dehalogenase that has dehalogenase activity and forms a covalent bond with a dehalogenase substrate, said mutant dehalogenase having at least 85% amino acid sequence identity to the polypeptide of SEQ ID NO: 1, wherein said mutant dehalogenase further comprises (a) substitutions corresponding to substitutions S58T, D78G, A155T, A172T, K175M, C176G, A224E, P291S, and A292T in SEQ ID NO: 1, and (b) substitutions at positions corresponding to positions 272 and/or 106 of SEQ ID NO: 1.