Patent ID: 9663829
Date: 2017-05-30
CPC Classifications: C12Q

Claim:
1. A combination of at least two oligomers for detecting in a sample a Gardnerella vaginalis (GV) 16S rRNA or a gene encoding the 16S rRNA, the oligomer combination comprising: first and second amplification oligomers for amplifying a GV nucleic acid target region corresponding to the GV 16S rRNA or the gene encoding the 16S-rRNA, wherein (a) the first amplification oligomer comprises a nucleotide sequence, wherein the nucleotide sequence consists of a first target-hybridizing sequence selected from the group consisting of SEQ ID NO:7 and SEQ ID NO:8, and wherein the first amplification oligomer does not comprise an additional target hybridizing sequence; and (b) the second amplification oligomer is a promoter primer or promoter provider and comprises a first region nucleotide sequence and a second region nucleotide sequence, wherein (i) the first region nucleotide sequence consists of a second target-hybridizing sequence selected from the group consisting of SEQ ID NO:36, SEQ ID NO:38, and SEQ ID NO:39, and (ii) the second region nucleotide sequence consists of a promoter sequence located 5′ to the second target-hybridizing sequence, and wherein the second amplification oligomer does not comprise an additional target hybridizing sequence.