Patent ID: 9534241
Date: 2017-01-03
CPC Classifications: C12N,C12P,C12Y

Claim:
1. A method of preparing a benzylisoquinoline alkaloid product, the method comprising: culturing an engineered microbial cell under conditions suitable for protein production, wherein the engineered microbial cell is an opioid-producing engineered microbial cell, wherein the engineered microbial cell comprises a plurality of heterologous coding sequences for encoding a plurality of enzymes within a pathway for producing the benzylisoquinoline alkaloid product, and wherein at least one heterologous coding sequence of the plurality of heterologous coding sequences encodes an enzyme that is within a pathway that converts a thebaine to the benzylisoquinoline alkaloid product wherein the engineered microbial cell is a yeast cell: adding a starting compound to the cell culture; and recovering the benzylisoquinoline alkaloid product from the cell culture, wherein the benzylisoquinoline alkaloid product is selected from the group consisting of a neopine, neomorphine, codeinone, codeine, morphine, morphinone, hydrocodone, dihydrocodeine, oxycodone, 14-hydroxycodeine, hydromorphone, and dihydromorphine.