Patent ID: 9587257
Date: 2017-03-07
CPC Classifications: C08F,C12N,C12P,C12Y

Claim:
1. A functional esterase mutant of the esterase of SEQ ID NO: 1, wherein said functional esterase mutant has: i) comparable or increased activity with respect to the hydrolysis of polyacrylic acid esters relative to the esterase of SEQ ID NO: 1; ii) increased stability relative to SEQ ID NO: 1; and/or iii) increased hydrolytic activity against at least one of polyacrylic acid methyl esters or polyacrylic acid butyl relative to the esterase of SEQ ID NO: 1; and wherein said functional esterase mutant is: a) a mutant of the esterase of SEQ ID NO: 1 and comprises a mutation in one or more of the amino acid residues selected from Ser17, Gly132, Trp134, Arg155, Glu251, Ala311 and Glu316; b) a mutant of the esterase of SEQ ID NO: 1 and comprises at least one of the mutations selected from Ser17Leu, Gly132Ser, Glu251Gly, Ala311Val and Glu316Lys, c) a mutant of the esterase of SEQ ID NO: 1 and comprises at least one of the mutations selected from Pro8Leu, Gly132Ser, Trp134Arg, Arg155Cys, Glu251Gly, Ala311Val and Glu316Lys; or d) a mutant of the esterase of SEQ ID NO: 1 and comprises the amino acid sequence of SEQ ID NO: 37 or SEQ ID NO: 38.