Patent ID: 9657302
Date: 2017-05-23
CPC Classifications: C07K,C12N

Claim:
1. A plastid transformation vector for stably transforming a target plastid genome, said vector comprising, as operably-linked components, a flanking sequence homologous to a trnI region in the plastid genome, a construct comprising a regulatory sequence comprising a 16S rRNA promoter operative in a plastid genome operably-linked to a light regulated psbA 5′ untranslated region element operably-linked to a DNA sequence coding for an IFNα2b polypeptide operably-linked to a psbA 3′ untranslated region element, and a flanking sequence homologous to a trnA region in the plastid genome, wherein said flanking sequences stably integrate the construct into an inverted repeat region of the target plastid genome through homologous recombination, and wherein said vector further comprises DNA sequence encoding a betaine aldehyde dehydrogenase (BADH) selectable marker.