Patent ID: 9732328
Date: 2017-08-15
CPC Classifications: C12N,C12Q,C12Y

Claim:
1. A multiplexed luciferase assay composition comprising multiple luciferase reporters, wherein at least two of the luciferase reporters are selected from the group consisting of a firefly luciferase, a Renilla luciferase, a Gaussia luciferase, and a Cypridina ( Vargula ) luciferase; wherein the different luciferase reporters emit at different wavelengths and/or utilize different substrates; and wherein: the firefly luciferase is a red-emitting human codon optimized luciferase encoded by a nucleotide sequence comprising SEQ ID NO:3; or the firefly luciferase is a green-emitting human codon optimized luciferase encoded by a nucleotide sequence comprising SEQ ID NO:4: or the the the the wherein at least one of the luciferase reporters comprises a secretory signal comprising SEQ ID NO: 7.