Patent ID: 9725516
Date: 2017-08-08
CPC Classifications: C07K,C12N

Claim:
1. A recombinant gram-negative bacterial cell comprising the following genetic modifications: (a) an expression vector comprising a recombinant polynucleotide encoding a protein comprising DsbC and a histidine-tag, said histidine tag being at the N-terminus or C-terminus of DsbC; (b) a polynucleotide encoding a reduced periplasmic Prc protease (Tsp) activity compared to a wild-type cell; (c) one or more polynucleotides encoding an antibody or an antigen binding fragment thereof specifically binding to CD154; and (d) optionally, a polynucleotide encoding a mutant extragenic suppressor (spr) protein, wherein the cell is isogenic to a wild-type bacterial cell except for the genetic modifications (a) to (c) and when present, (d).