Patent ID: 9587008
Date: 2017-03-07
CPC Classifications: A61K,A61P,C07K,C12N,C12P

Claim:
1. A method of producing a vitamin K dependent protein, the method comprising the steps of: (a) transfecting a population of mammalian cells with a gene encoding the vitamin K dependent protein operably linked to a promoter, wherein the mammalian cells do not contain heterologous genetic material encoding proteins involved in the post-translation modification of the vitamin K dependent protein; (b) performing at least one round of cloning and screening to identify cells which produce at least 30 mg/L of the vitamin K dependent protein, wherein at least 10% of the vitamin K dependent protein is biologically active; (c) optionally, repeating step (b) one or more times; and (d) harvesting the vitamin K dependent protein.