Patent ID: 9732373
Date: 2017-08-15
CPC Classifications: C12N,C12Q,C12Y,G01N

Claim:
1. An isolated polynucleotide encoding a click beetle red luciferase (CBR) variant polypeptide having at least 80% amino acid sequence identity to SEQ ID NO: 1 and comprising an amino acid substitution at a position corresponding to positions 389, 444, and 251 of SEQ ID NO: 1, or an amino acid substitution at a position corresponding to positions 334 and 351 of SEQ ID NO: 1, wherein the variant CBR polypeptide has at least one of enhanced luminescence, altered light emission wavelength, altered substrate specificity, or a combination thereof, as compared to a CBR polypeptide of SEQ ID NO: 1.