Patent ID: 9695465
Date: 2017-07-04
CPC Classifications: C12N,C12Q

Claim:
1. A method for isolating nucleic acids from a sample, comprising: a) obtaining a sample that has been stabilised by the use of at least one cationic detergent, wherein the cationic detergent has formed complexes with the nucleic acids; b) obtaining the complexes optionally together with other sample components from the stabilised sample, wherein said complexes comprise the nucleic acids to be isolated; c) resuspending the complexes and optionally adding one or more additives before, during and/or after resuspension, thereby obtaining a resuspended sample comprising at least d) isolating nucleic acids from the resuspended sample wherein a plurality of samples is prepared according to steps a) to c) thereby providing a plurality of resuspended samples, wherein the resuspended, samples are divided into batches, the nucleic acids are isolated from the batches according to step d), and holding times between steps c) and d) vary at least between two batches.