Patent ID: 9694036
Date: 2017-07-04
CPC Classifications: A61J,A61K,A61P,B65D,C12N,C12Q,C12Y,G01N

Claim:
1. A method for providing an enriched population of midbrain dopaminergic (DA) neurons comprising: differentiating cells of a population of human induced pluripotent cells to provide a neural lineage cell population; further differentiating cells of the neural lineage cell population to generate a cell population which includes midbrain neurons; and purifying cells from said cell population using a transgenic screenable or selectable marker under the control of a pan-neural promoter expressed by cells of the cell population, to provide an enriched population of midbrain DA neurons, wherein the differentiation does not comprise culturing the cells in a medium containing exogenously added FGF8 and further wherein said differentiation steps are carried out in a chemically defined medium that is free of feeder cells and feeder cell extracts and at least 80% of cells in the enriched population are positive for both Lmx1 and FoxA2 expression.