Patent ID: 9663768
Date: 2017-05-30
CPC Classifications: C12N,C12P,C12Y

Claim:
1. A microbial system for the production of 5-hydroxytryptophan (5-HTP) comprising a plurality of genetically engineered cells comprising: a first genetically engineered cell comprising (i) at least one shikimate pathway enzyme, (ii) at least one enzyme having anthranilate synthase activity, and (iii) at least one enzyme having salicylate 5-hydroxylase (S5H) activity, which enzyme further has activity toward anthranilate (AA); and a second genetically engineered cell comprising at least one enzyme having tryptophan biosynthesis activity, wherein the enzyme catalyzes the conversion of anthranilate (AA) to tryptophan and further has activity toward 5-hydroxyanthranilate (5-HAA).