Patent ID: 9650663
Date: 2017-05-16
CPC Classifications: C12N,C12Q

Claim:
1. A method for determining a stem cell differentiation, comprising the steps of: (a) preparing the sensor for detecting the embryonic stem cell differentiation, said sensor comprising (i) an electrode which comprises a gold electrode as the working electrode, a platinum electrode as the counter electrode, and a Ag/AgCl electrode as the reference electrode; and (ii) 1-naphthyl phosphate (1-NP), a substrate for an alkaline phosphatase; (b) contacting the embryonic stem cells of interest to the sensor; and (c) measuring the phosphorylation or dephosphorylation of 1-NP for alkaline phosphatase on the cell through an electrical current signal which peaks at about 0.1 V, wherein the peak changes to negative voltage as the concentration of 1-NP decreases; and wherein if the embryonic stem cell comprises an alkaline phosphatase, the embryonic stem cell is an undifferentiated stem cell; and if the embryonic stem cell comprises no alkaline phosphatase, the embryonic stem cell is a differentiated stem cell.