Patent ID: 9683207
Date: 2017-06-20
CPC Classifications: C12M

Claim:
1. A method for growing cells using an apparatus having at least two reservoirs, an enclosed chamber, and at least one hollow fiber, wherein the at least one hollow fiber is disposed within the enclosed chamber such that an extra-capillary space is defined between an interior of the enclosed chamber and an exterior surface of the at least one hollow fiber, and the at least one hollow fiber has a lumen passing therethrough, the method comprising: placing cells into the extra-capillary space; providing the liquid media in at least one of the at least two reservoirs; providing the gas in at least one of the at least two reservoirs; and generating alternating directed flows of the liquid media and of the gas through the lumen of the at least one hollow fiber, whereby the liquid media and the gas are capable of providing nutrients and/or oxygen through walls of the at least one hollow fiber to nourish the cells in the extra-capillary space.