Patent ID: 9580462
Date: 2017-02-28
CPC Classifications: C07K

Claim:
1. A method for isolating polypeptides from cells comprising: contacting cells with a lysis buffer, wherein cell lysis occurs resulting in a viscous cell lysate, and wherein the cell lysis buffer comprises a surfactant, a detergent, or a combination thereof, and a metal chelator; passing the viscous cell lysate through a porous filtering medium, wherein genomic DNA and debris are retained by the porous filtering medium and polypeptides present in the cell lysate pass through the porous filtering medium, and wherein the porous filtering medium comprises a pore size of at least 10 to no greater than 60 microns and a thickness of at least 0.5 millimeter to no greater than 20 millimeters; and collecting the filtrate, wherein the filtrate comprises a non-viscous polypeptide extract comprising isolated polypeptides, wherein the isolated polypeptides comprise the polypeptides present in the cell lysate.