Patent ID: 9689875
Date: 2017-06-27
CPC Classifications: G01N

Claim:
1. A method of detecting CD3 protein and CD16 protein in a sample, comprising: contacting the sample with a buffer comprising Tris, a preservative, wherein the pH of the buffer is about 8-9; contacting the sample with a CD3 antibody; contacting the sample with a labeled secondary antibody to permit detection of the CD3 antibody; denaturing the sample by incubating the sample at 80° C.-100° C. for at least 5 minutes; contacting the sample with a blocking antibody; contacting the sample with the buffer; contacting the sample with a CD16 antibody; contacting the sample with a labeled secondary antibody to permit detection of the CD16 antibody; and determining that CD3 protein is present in the sample if the labeled secondary antibody to permit detection of the CD3 antibody is detected, determining that CD16 protein is present in the sample if the labeled secondary antibody to permit detection of the CD16 antibody is detected, or combinations thereof.