Patent ID: 9598724
Date: 2017-03-21
CPC Classifications: C12Q

Claim:
1. A reaction mixture for multiple displacement amplification of a nucleic acid sample consisting of: a set of oligonucleotide primers wherein at least one primer of said set of oligonucleotide primers comprises one or more universal nucleobases, and at least one primer of said set of oligonucleotide primers comprises one or more phosphorothioate linkages, or one or more nucleotide residues with a ribose modification that stabilizes a 3′-endo conformation of said ribose ring or locks said ribose ring in said 3′-endo conformation or a combination thereof; a plurality of enzymes having enzyme activity, wherein said enzymes comprise Phi29 polymerase, DNA polymerase I, and pyrophosphatase; a set of natural deoxynucleotide triphosphates; and one or more compatible solutes.