Patent ID: 9618511
Date: 2017-04-11
CPC Classifications: C12Q,G01N

Claim:
1. A method for detecting Akt (protein kinase B, PKB) activation in a cell, comprising expressing a heterologous Aβ42 polypeptide in a cell in vitro using recombinant cDNA or by viral vector, lysing the cell to produce an Akt containing lysate, and determining whether Akt is activated by ELISA or Western blot by detecting whether or not Akt is phosphorylated (on serine 473 or threonine 308), wherein the lysate is fractionated using gel electrophoresis and phosphorylated forms of Akt are directly identified by Western blot or Akt is immobilized onto a solid support and activation is determined using antibodies by ELISA or Akt is immunoprecipitated onto beads in solution from the lysates prepared from the cells and then subjected to Western blot.