Patent ID: 9708622
Date: 2017-07-18
CPC Classifications: A24B,A24D,C07K,C12N

Claim:
1. A method of decreasing nitrate concentration in leaves of a test tobacco plant to below that of the corresponding nitrate concentration in leaves of a wild-type tobacco plant cultured under the same conditions, the method comprising: (i) transforming a plant cell of a test tobacco plant with a genetic construct, or a vector comprising said genetic construct, the construct comprising a promoter operably linked to a coding sequence encoding a polypeptide having nitrate transporter activity, wherein the polypeptide comprises the amino acid sequence as set out in SEQ ID No.3, or an amino acid sequence which has at least 95% sequence identity therewith, wherein the promoter is a Carnation Etched Ring Vims (CERV) promoter; and (ii) regenerating a tobacco plant from the transformed cell, wherein the regenerated transformed tobacco plant expresses said construct and has decreased nitrate concentration in its leaves when compared to the wild-type tobacco plant.