Patent ID: 9534257
Date: 2017-01-03
CPC Classifications: C12Q

Claim:
1. A method of identifying target nucleic acid sequences in a sample, comprising: (a) generating from a sample a plurality of first template molecules and a plurality of second template molecules, wherein said first template molecules comprise a sequence of a first target nucleic acid and a first identity sequence (IS) tag, and said second template molecules comprise a sequence of a second target nucleic acid and a second IS tag, and wherein said first IS tag comprises a first identification (ID) code and said second IS tag comprises a second ID code; (b) generating at least one cluster of nucleic acid amplicons by nucleic acid amplification of said first template molecules and at least one cluster of nucleic acid amplicons by clonal amplification of said second template molecules in parallel individual reactions on a surface, wherein said clusters of nucleic acid amplicons are spatially separated; and (c) identifying said ID codes of said IS tags of said nucleic acid amplicons on said surface to determine the target nucleic acid sequences represented by said nucleic acid amplicons and the step (c) of identifying said ID codes of said IS tags of said nucleic acid amplicons further comprises: