Patent ID: 9725693
Date: 2017-08-08
CPC Classifications: A61K,A61L,C12N

Claim:
1. A method of making a biologically active three-dimensional scaffold capable of supporting growth and differentiation of a cell, the method comprising: providing an unfractionated extracellular matrix extract from a mammalian tissue in a form selected from the group consisting of a powder, a solution, a hydrogel and a suspension; placing the unfractionated extracellular matrix extract in a mold; freezing the unfractionated extra cellular matrix extract at 0° C. or below at a controlled freezing rate of less than about 1° C./min; freeze-drying the unfractionated extra cellular matrix extract by subjecting the unfractionated extracellular matrix extract to a temperature between about −130° C. and about −80° C. for a time sufficient to remove at least 90% of a liquid from the unfractionated extracellular matrix extract and thereby obtaining a lyophilized unfractionated extracellular matrix extract; and optionally crosslinking the lyophilized unfractionated extracellular matrix extract by contacting with a crosslinker and thereby providing the biologically active three-dimensional scaffold having porous morphology.