Patent ID: 9555009
Date: 2017-01-31
CPC Classifications: A61K

Claim:
1. A method of preparing ciprofloxacin loaded biodegradable polymeric nanoparticles comprising: a. preparing a 1% to 3% solution of diethyl amino ethyl cellulose in ethyl acetate by stirring ethyl amino ethyl cellulose in ethyl acetate for 15 to 25 minutes at medium speed; b. adding a 0.2-0.6% aqueous solution of ciprofloxacin, drop by drop, to the solution in step (a) and mixing in an ultrasonic shaker; c. adding a 0.5 to 1% aqueous solution of polyvinyl alcohol drop by drop to step (b) solution and emulsifying by subjecting the solution to ultrasonification at 50-70 Hz for 15-25 minutes; d. subjecting the solution in step (c) to ultracentrifugation for 5-15 minutes at 10-15,000 rpm; and, e. removing the sediment from step (d) and drying at 45-55° C. for 0.5 to 1.5 hours.