Patent ID: 9663810
Date: 2017-05-30
CPC Classifications: C07K,C12N,C12P,G01N

Claim:
1. A method of producing a recombinant protein preparation having a target value of fucosylated glycans, the method comprising: (a) providing a cell genetically engineered to express a recombinant protein; (b) culturing the cell in a culture medium comprising 0.1 mg/L to 10 mg/L putrescine under conditions in which the cell expresses the recombinant protein; and (c) harvesting a preparation of the recombinant protein produced by the cell that meets the target value of the fucosylated glycans wherein the target value of fucosylated glycans is a level at least 10% higher than a level of fucosylated glycans in a preparation produced by culturing the cell in the medium not comprising 0.1 mg/L to 10 mg/L putrescine.