Patent ID: 9598719
Date: 2017-03-21
CPC Classifications: C12N,C12Q,G16B

Claim:
1. A method for determining the binding affinity of a candidate compound for G-quadruplex structures comprising the steps of: (a) providing a G-quadruplex polynucleotide analog, said G-quadruplex polynucleotide analog having a G-quadruplex structure and at least one intrinsic fluorescent moiety; (b) contacting said G-quadruplex polynucleotide analog with said candidate compound; (c) measuring a difference in a fluorescence property of said G-quadruplex polynucleotide analog; and (d) determining the binding affinity of the candidate compound; wherein the G-quadruplex analog polynucleotide comprises: a nucleobase sequence that is an analog of a telomeric repeat sequence; and at least one nucleotide comprising a 6-methyl-isoxanthopterin nucleobase; wherein: said G-quadruplex analog polynucleotide forms a G-quadruplex structure; and each 6-methyl-isoxanthopterin nucleobase is located within said G-quadruplex structure.