Patent ID: 9702822
Date: 2017-07-11
CPC Classifications: C12Q,G01N

Claim:
1. A method of performing a digital assay, the method comprising: partitioning a same reaction mixture into a plurality of droplets, the reaction mixture containing a first template for a first amplicon, a second template for a second amplicon, two or more primers to generate the first and second amplicons from their corresponding templates, and an intercalating dye, wherein only a subset of the droplets contain a copy of the first template and only a subset of the droplets contain a copy of the second template; amplifying nucleic acid in the droplets to generate the first amplicon from the first template and the second amplicon from the second template, wherein the first amplicon and the second amplicon are of different length from one another; detecting an intensity of light emitted by the intercalating dye from individual droplets while the intercalating dye remains bound to the first amplicon and the second amplicon; and comparing the intensity of light detected from each individual droplet to one or more threshold values to classify each individual droplet as negative for both amplicons, positive only for the first amplicon, or positive for the second amplicon.