Patent ID: 9580491
Date: 2017-02-28
CPC Classifications: A01K,C07K,C12N

Claim:
1. An in vitro method of producing a mammalian cell whose genome comprises a synthetic transgene that encodes a chimeric immunoglobulin heavy chain, wherein said immunoglobulin heavy chain comprises a chimeric constant region, said method comprising the steps of: designing one or more DNA constructs in silico, wherein said one or more constructs comprise polynucleotide sequences comprising one or more human V, D and/or J gene segments and a chimeric constant region comprising a human CH1 gene segment, a human upper hinge region gene segment, a non-human CH2 gene segment, and a non-human CH3 segment; producing said one or more DNA constructs; and introducing the one or more constructs into the genome of a mammalian cell, wherein the genome of the mammalian cell comprises the synthetic transgene that encodes the chimeric immunoglobulin heavy chain comprising the chimeric constant region comprising a human CH1, a human upper hinge region, a non-human CH2, and a non-human CH3.