Patent ID: 9719108
Date: 2017-08-01
CPC Classifications: C12N,C12Y

Claim:
1. A method of introducing a sequence specific nuclease (SSN) into a plant cell comprising an intact cell wall, the method comprising: coating a nanoparticle with a SSN, wherein the nanoparticle has at least one dimension less than 100 nm; placing a cell having a cell wall and the coated nanoparticle in contact with each other; and allowing uptake of the nanoparticle and the SSN into the plant cell; wherein the nanoparticle is selected from the group consisting of porous nanoparticles, mesoporous nanoparticles, silica nanoparticles, polymer nanoparticles, tungsten nanoparticles, gelatin nanoparticles, nanoshells, nanocores, nanospheres, nanorods, magnetic nanoparticles, semiconductor nanoparticles, nanomatrices, dendrimeric nanomatrices and combinations thereof.