Patent ID: 9617533
Date: 2017-04-11
CPC Classifications: C12N,C12P

Claim:
1. A method for isolating a nucleic acid that encodes a polypeptide that binds to a target substance, comprising the following steps: (a) translating an mRNA into a polypeptide in a composition that possesses cell-free protein synthesis activity to form a complex comprising the mRNA and the polypeptide, (b) contacting the complex formed in (a) with the target substance, and (c) recovering the complex bound to the target substance, and isolating the mRNA that constitutes the recovered complex or a cDNA thereof as a nucleic acid that encodes a polypeptide that binds to the target substance, wherein the composition comprises an independently purified initiation factor, an independently purified elongation factor, an independently purified aminoacyl-tRNA synthetase, an independently purified ribosome, an independently purified amino acid, an independently purified nucleoside triphosphate, and an independently purified tRNA, wherein the composition is prepared by mixing at least the independently purified initiation factor, the independently purified elongation factor, the independently purified aminoacyl-tRNA synthetase, and the independently purified ribosome, wherein the initiation factor, the elongation factor, the aminoacyl-tRNA synthetase, and the ribosome each were purified from a Gram-negative bacteria, wherein the composition has a ribosome concentration of 0.01-50 μM, and wherein the composition has a lipopolysaccharide concentration of 1.0×10