Patent ID: 9605242
Date: 2017-03-28
CPC Classifications: A61K,C07K,C12N

Claim:
1. An ex vivo method for removal of tumor cells from blood of a tumor patient, the method comprising: providing blood suspected of containing tumor cells intra-operatively salvaged from a tumor patient; contacting the intra-operatively salvaged blood for a time period of between 10 and 180 minutes with a crosslinking agent capable of forming a three-dimensional network comprising the tumor cells, wherein the crosslinking agent comprises:  and further wherein the time period of between 10 and 180 minutes is sufficient to form associates by cross-linking tumor cells present in the intra-operatively salvaged blood with the at least one antibody, the at least one scaffold protein, or the combination thereof of a size sufficient to be retained by filtration or to be separated by centrifugation, optionally wherein the associates further comprise cross-linked immune cells and/or other tumor cells; mechanically removing the associates from the intra-operatively salvaged blood; and readministering the intra-operatively salvaged blood to the patient after mechanically removing the associates.