Patent ID: 9611486
Date: 2017-04-04
CPC Classifications: C07K,C12N

Claim:
1. A eukaryotic expression vector comprising a nucleotide sequence encoding a fusion protein comprising a 1st polypeptide which is a DNA binding protein and which belongs to the partitioning system of bacterial DNA wherein the binding to DNA is solely dependent on the DNA binding protein belonging to the partitioning system of bacterial DNA used and on the recognition site at the DNA, a derivative of a DNA binding protein belonging to the partitioning system of bacterial DNA or a fragment of a DNA binding protein belonging to the partitioning system of bacterial DNA, wherein the derivative of a DNA binding protein belonging to the partitioning system of bacterial DNA has at least 80% identity with the sequence of a DNA binding protein belonging to the partitioning system of bacterial DNA, contains at least the motif involved in DNA binding of the DNA binding protein belonging to the partitioning system of bacterial DNA, and is capable of specifically binding to DNA wherein the binding to DNA is solely dependent on the derivative used and on the recognition site at the DNA, wherein the fragment of a DNA binding protein belonging to the partitioning system of bacterial DNA has one amino acid less at the C-terminal end and/or N-terminal end compared with the DNA binding protein belonging to the partitioning system of bacterial DNA and contains at least the motif involved in DNA binding of the DNA binding protein belonging to the partitioning system of bacterial DNA, and wherein said DNA binding protein which belongs to the partitioning system of bacterial DNA is different from the ParB protein of the P1 plasmid of