Patent ID: 9657065
Date: 2017-05-23
CPC Classifications: C07K,C12N

Claim:
1. A process for the purification of a VLP from a recombinant bacterial host expressing said VLP, wherein said VLP is a VLP of RNA bacteriophage Qβ comprising coat protein of SEQ ID NO:3, the process comprising the steps of: (a) homogenizing said bacterial host; (b) clarifying the homogenate obtained by said homogenizing; (c) purifying said VLP from the clarified homogenate obtained by said clarifying in a first chromatography comprising the steps of: (d) further purifying said VLP from the eluate obtained by said first chromatography in a second chromatography, wherein said second chromatography is performed on a second chromatography matrix, wherein said second chromatography matrix is a hydroxyapatite matrix; wherein said steps are performed in the given order.