Patent ID: 9695434
Date: 2017-07-04
CPC Classifications: A01H,C11B,C12N

Claim:
1. A Brassica plant comprising a modified allele at a fatty acid desaturase 3D (fad3D) locus, a fatty acid desaturase 3E (fad3E) locus, a fatty acid desaturase 3A (fad3A) locus and a fatty acid desaturase 3B (fad3B) locus wherein: (i) said modified allele results in the production of a FAD3 polypeptide having reduced desaturase activity relative to a corresponding wild-type FAD3 polypeptide; (ii) said fad3D locus has at least 90% identity to SEQ ID NO:32; (iii) said fad3E locus has at least 90% identity to SEQ ID NO:1; (iv) said fad3A locus comprises a nucleic acid selected from the group consisting of (a) a nucleic acid encoding a FAD3A polypeptide having a cysteine substituted for arginine at position 275 and (h) a nucleic acid encoding a truncated FAD3A polypeptide; (v) said fad3B locus comprises a nucleic acid selected from the group consisting of (a) a nucleic acid having a mutation in an exon-intron splice site recognition sequence and (b) a nucleic acid encoding a truncated FAD3B polypeptide and (vi) wherein said plant produces seeds yielding an oil having a stearic acid content of 0.95% to 3.9% and an alpha-linolenic acid (ALA) content of 0.5% to 1.95%.