Patent ID: 9546394
Date: 2017-01-17
CPC Classifications: C12Q,G01N

Claim:
1. A reagent for use in an assay for the simultaneous, rapid measurement of at least two of total cholesterol, low density lipoprotein cholesterol (LDL-C) and high density lipoprotein cholesterol (HDL-C) in lipoproteins in a sample of blood from a subject without substantial precipitation of said lipoproteins, consisting of a sodium phosphate buffer, between about 0.01% and about 1% Triton X-100, between about 0.5 g/L and about 20 g/L pluronic L64, between about 0.5 mM and about 20 mM N-Ethyl-N-(2-hydroxy-3-sulfopropyl)-3,5-dimethoxyaniline (ALPS), horse-radish peroxidase (HRP), cholesterol esterase from Pseudomonas sp. , and cholesterol oxidase from Pseudomonas sp.