Patent ID: 9579374
Date: 2017-02-28
CPC Classifications: A61K,C07K,C12N,G01N

Claim:
1. A method for selecting influenza viral lineages for use in preparing a pharmaceutical preparation for preventing influenza viral infection, the method comprising: (a) isolating an influenza targeted virus strain from a first animal; (b) serial passaging a plurality of samples of the influenza targeted virus strain from the first animal in respective droplets in cells of a second animal, wherein the influenza targeted virus strain adapts to the cells of the second animal to form second animal cell-adapted viral lineages; (c) in parallel to the serial passaging, synthesizing antibodies that neutralize the influenza targeted virus strain in a third animal, wherein the synthesizing comprises: (d) running at least one of a drop-based neutralization assay or drop-based affinity assay on the second animal cell-adapted viral lineages; and (e) selecting the second animal cell-adapted viral lineages that are neutralized by the antibodies to identify second animal cell-adapted viral lineages having antigenic similarity to the influenza targeted virus strain.