Patent ID: 9693720
Date: 2017-07-04
CPC Classifications: A61B

Claim:
1. A sensor for determining pH and glucose concentration comprising: an optical fiber comprising a fluorescence chemical indicator, the chemical indicator system comprising a single type of fluorophore that generates a fluorescence emission signal when excited by light and a glucose binding moiety to modulate the florescence emission signal, wherein the fluorophore is sensitive to both pH and glucose concentration when the fluorophore is operably coupled to the glucose binding moiety and the extent of modulation of the fluorescence emission signal by the glucose binding moiety is related to the amount of glucose bound to the glucose binding moiety, and wherein the fluorophore comprises an acid form that absorbs light at a first excitation wavelength and a base form that absorbs light at a second excitation wavelength; a light source configured, to deliver the first excitation wavelength and the second excitation wavelength; a detector configured to detect a first fluorescence emission signal generated by the fluorophore in response to the first excitation wavelength and a second fluorescence emission signal generated by the fluorophore in response to the second excitation wavelength, wherein the first and second fluorescence emission signal are each dependent on pH; and a controller configured to monitor emission signals from the detector, to ratiometrically determine the pH using the first and second fluorescence emission signals and to determine the glucose concentration using one of the first fluorescence emission signal and the second fluorescence emission signal, wherein the determining of the pH and the determining of the glucose concentration are performed simultaneously, and to correct the determined glucose concentration based on the determined pH, wherein the fluorophore is HPTS-triCysMA.