Patent ID: 9675643
Date: 2017-06-13
CPC Classifications: A01N,A61K,A61L,C08L,C12N

Claim:
1. A biological composition suitable for implantation or grafting to a human patient comprising: a mixture of mechanically selected biologic material derived from bone marrow from cut vertebral bodies including cortical and cancellous bone having non-whole cellular components including each of vesicular components and active and inactive components of biological activity, cell fragments, cellular excretions, and extracellular components wherein the mixture is cleaned and packed for processing in a cold media of DMEM without L-glutamine or HEPES; a protectant or cryoprotectant of polyampholyte added to the mixture; and wherein the mixture is intermixed with the protectant or cryoprotectant of polyampholyte to form a coating on the non-whole cellular components of the mixture prior to preservation or cryopreservation and is subsequently frozen, the frozen mixture with the protectant or cryoprotectant of polyampholyte being preserved frozen, the frozen mixture and the protectant or cryoprotectant of polyampholyte when thawed is prepared for implantation is configured to thaw into a liquid for direct implantation in the absence of washing the protectant or cryoprotectant from the mixture which is compatible with biologic function.