Patent ID: 9541542
Date: 2017-01-10
CPC Classifications: C12Q,G01N

Claim:
1. A method of detecting filarial larvae in blood without using an immunological technique, comprising: without using an immunological technique, preparing a measurement sample from a blood sample collected from an animal; flowing the prepared measurement sample through a flow cell; irradiating light to the measurement sample flowing through the flow cell; detecting first scattered light, second scattered light different from the first scattered light and fluorescent light all scattered from the measurement sample; converting the first and second scattered light and the fluorescent light, respectively, into a first series of pulse signals, a second series of pulse signals and a third series of pulse signals, the pulse signals each being representative of an event indicative of passing of a particle contained in the measurement sample, wherein each event is valued with a width of a corresponding pulse and a height thereof; gating, for distinguishing filarial larvae from white blood cells in the measurement sample, events represented by at least one of the first, second and third series of pulse signals to identify a plurality of discrete populations of events to thereby detect a first group of events indicative of passing of filarial larvae contained in the measurement sample and a second group of events indicative of passing of white blood cells in the measurement sample counting the first group of events indicative of passing of filarial larvae contained in the measurement sample; and displaying a count of the first group of events, wherein gating events represented by at least one of the first, second and third series of pulse signals comprises (i) gating the events in a one-dimensional distribution of widths represented by one of the first, second and third series of pulse signals, (ii) gating the events in a two-dimensional distribution of two groups of heights represented, respectively, by two of the first, second and third series of pulse signals or (iii) gating the events in a two-dimensional distribution of widths and heights represented by the first series of pulse signals.