Patent ID: 9662383
Date: 2017-05-30
CPC Classifications: A61K,C07K,C12N,C12Y

Claim:
1. A method of producing influenza virus bearing multiple α-Gal epitopes, comprising: a) providing; i) an influenza virus; and ii) a transgenic host cell susceptible to infection by said influenza virus and comprising a recombinant nucleic acid sequence that is stably transfected in the genome of said transgenic host cell, wherein said recombinant nucleic acid sequence comprises a nucleotide sequence encoding α1,3galactosyltransferase (α1,3GT) in operable combination with a promoter, and wherein said transgenic host cell expresses a higher level of α1,3GT compared to a host cell lacking said recombinant nucleic acid sequence; and b) inoculating said transgenic host cell with said influenza virus to produce an inoculated host cell, wherein said inoculated host cell produces an influenza virus bearing multiple α-Gal epitopes.