Patent ID: 9678005
Date: 2017-06-13
CPC Classifications: B01L,G01N

Claim:
1. A method of detecting Escherichia coli ( E. coli ) in a vegetable sample using a portable microfluidic device, the vegetable sample comprising a vegetable component in a buffer, the method comprises: mixing via diffusional mixing in a first well of a light transparent base of the portable microfluidic device both a first bead solution and a portion of the vegetable sample to create a first mixture, the first bead suspension comprises beads conjugated with an antibody specific for mixing via diffusional mixing in a second well of the light transparent base of the portable microfluidic device both a second bead solution and a portion of the vegetable sample to create a second mixture, the second bead suspension comprises beads that are not conjugated with the antibody specific for irradiating the first well and the second well with incident light using a light disposed under the first well and the second well, the incident light having a wavelength of 650 nm; detecting using a detector forward scattered light at a 45 degree angle with respect to the incident light scattered by the first mixture and forward scattered light at a 45 degree angle with respect to the incident light scattered by the second mixture, the detector is operatively connected to a processing unit, the processing unit comprises an operational amplifier circuit and calculates I from the forward scattered light scattered by the first mixture and calculates I determining if