Patent ID: 9701998
Date: 2017-07-11
CPC Classifications: C12Q

Claim:
1. A method of nucleic acid sequence analysis, comprising: (a) providing a plurality of partitions comprising a plurality of polynucleotide molecules and a plurality of barcode molecules, wherein: i. said plurality of partitions comprises at least 1,000 partitions; ii. said plurality of barcode molecules comprises at least 1,000 different nucleic acid barcode molecules having different barcode sequences; iii. said at least 1,000 different nucleic acid barcode molecules are disposed within separate partitions of said at least 1,000 partitions, such that each of said at least 1,000 partitions has a different barcode sequence of said different barcode sequences; and iv. said at least 1,000 partitions are at least 1% of said plurality of partitions; (b) subsequent to (a), generating a plurality of barcoded molecules from said plurality of polynucleotide molecules and said plurality of barcode molecules within said plurality of partitions; and (c) directing said plurality of barcoded molecules generated in (b) to nucleic acid sequencing, wherein a sequence of a given polynucleotide of said plurality of polynucleotide molecules is determined.