Patent ID: 9636392
Date: 2017-05-02
CPC Classifications: A61K,C12N,C12P

Claim:
1. A method of producing a capsular polysaccharide having a pneumococcal serotype, the method comprising: (a) cultivating bacterial cells that produce a pneumococcal serotype while maintaining pH of a culture broth in the range of 7.0 to 9.4; (b) terminating the cultivating of step (a) at a time between when the absorbance of the culture broth remains constant and when the absorbance begins to decrease; (c) performing additional cultivating of the culture broth of step (b) without addition of a pH adjuster until the pH of the culture broth reaches pH of 5.5 or lower; (d) adding a lysing agent to the culture broth obtained from step (c) to lyse cells, precipitating proteins, and removing the precipitated proteins and cell debris to obtain a clarified cell lysate; and (e) isolating and purifying the capsular polysaccharide from the lysate obtained from step (d).