Patent ID: 9637753
Date: 2017-05-02
CPC Classifications: C07K,C12N

Claim:
1. A genetically transformed plant with one or more expression vectors comprising a seed-specific expression cassette and a nucleotide sequence encoding a mutein of human apolipoprotein (Apo) A-1, expressing in the seed's storage tissue one or more of said muteins, and forming oligomers comprising three or more monomers, in an essentially oligomeric form, said one or more muteins characterised in that they show in their oligomeric forms biological activities equal to or higher than the activities shown by apolipoprotein (Apo) A-1 Milano in its dimeric form in the following tests: a′) measurement of the reverse cholesterol transport capacity evaluated by calculation of the association kinetics of equal quantities of said oligomer, and of said dimer of Apo A-1 Milano with dimyristoylphosphatidylcholine (DMPC); and b′) measurement of the plasma half life of said oligomer by a plasma half life test in comparison to a calibration curve obtained performing the same test with the purified Apo A-1 Milano dimer.