Patent ID: 9574235
Date: 2017-02-21
CPC Classifications: C07H,C12N,C12P,C12Q

Claim:
1. A system for sequencing nucleic acid templates, the system comprising: (a) an array of sites, wherein each site comprises a first nucleic acid template and a second nucleic acid template, wherein the first nucleic acid template comprises a DNA primer bound to the first template, and wherein the second nucleic acid template comprises an RNA primer bound to the second template; (b) a DNA polymerase species suitable for extending the DNA primer bound to the first template; (c) an RNA polymerase suitable for extending the RNA primer bound to the second template; (d) a set of deoxyribonucleotide analogs suitable for producing a DNA primer extension product extending from the DNA primer, wherein the extension product comprises a deoxyribonucleotide analog at each of the sites; (e) a set of ribonucleotide analogs suitable for producing an RNA primer extension product extending from the RNA primer, wherein the extension product comprises a ribonucleotide analog at each of the sites; and (f) a detector for detecting the DNA primer extension product and the RNA primer extension product.