Patent ID: 9631002
Date: 2017-04-25
CPC Classifications: C07K,C12N,C12P,C12Q,C12Y,G01N

Claim:
1. A method of increasing the amount of carboxylated vitamin K dependent protein in a mammalian cell, comprising introducing, into a mammalian cell that expresses a first nucleic acid encoding a vitamin K dependent protein, a second nucleic acid comprising a heterologous nucleotide sequence encoding vitamin K epoxide reductase (VKOR), wherein the heterologous nucleotide sequence encoding VKOR is a nucleotide sequence that encodes a Nematostella VKOR, under conditions whereby said first and second nucleic acids are expressed to produce a vitamin K dependent protein and VKOR, respectively, thereby increasing the amount of carboxylated vitamin K dependent protein in the mammalian cell.