Patent ID: 9617590
Date: 2017-04-11
CPC Classifications: C12Q,G01N,Y10T

Claim:
1. A method for preparing a DNA molecule for sequencing, the method comprising: fragmenting the DNA molecule into double-stranded fragments; amplifying at least a portion of the double-stranded fragments; preparing an emulsion that comprises a first subset of droplets and a second subset of droplets, wherein: circularizing the fragments so that the first end of a fragment comprises a first loop connecting the strands of the fragment and the second end of the fragment comprises a second loop connecting the strands of the fragment; annealing a first sequencing primer to the first loop oriented to sequence at least a portion of one strand of at least one fragment; and annealing a second sequencing primer to the second loop oriented to sequence at least a portion of the other strand of the fragment.