Patent ID: 9695457
Date: 2017-07-04
CPC Classifications: C12Q,G01N,H01J

Claim:
1. A method for detection of the presence or absence of particular bacteria in a clinical sample, the method comprising: (i) enriching the clinical sample by culturing the bacteria present in the clinical sample for a period of time less than 6 hours such that pre-modification biomarkers are produced in the sample, wherein enrichment is carried out by: (ii) analysing the enriched clinical sample by mass spectrometry, to obtain clinical biomarker profile data, wherein the clinical biomarker profile data comprises peaks of specific masses in the region below 1500 Da, corresponding to pre-modification biomarkers produced by bacteria in the clinical sample; (iii) correlating the clinical sample biomarker profile data with reference data to look for peaks having specific masses in the region below 1500 Da common to the clinical sample biomarker profile data and to the reference data, to determine the presence of absence of said particular bacteria in the sample; wherein a match between the masses of the peaks of the clinical sample biomarker profile data the reference data indicates the presence of a particular bacteria in the clinical sample, wherein the reference data relates to pre-modification biomarkers represented by peaks having specific masses produced by a reference sample of said particular bacteria which has been cultured for less than six hours, and wherein the concentration of bacteria in the clinical sample before enrichment is about 10