Patent ID: 9644202
Date: 2017-05-09
CPC Classifications: C07K,C12N

Claim:
1. A switchable aptamer which is obtained through a method comprising: a) incubating a pool comprising a mixture of aptamers with a target ligand and a binding ion to form target-aptamer complexes comprising said target ligand and aptamers specific to said target; b) separating unbound aptamer molecules from the target-aptamer complexes; c) contacting the target-aptamer complexes with a chelating agent having affinity for said binding ion wherein a switchable aptamer specific to said target is released from the target-aptamer complexes; and d) isolating the switchable aptamer released in step c; wherein the switchable aptamer comprises a nucleotide sequence represented in any one of SEQ ID NOS: 31 through 47 or a nucleotide sequence with at least 70%, 75%, 80%, 85%, 90%, 95% or 99% identity with any one of SEQ ID NOS: 31 through 47.