Patent ID: 9695427
Date: 2017-07-04
CPC Classifications: C08B,C12N,C12P,C12Y

Claim:
1. A method of producing a polymer comprising the structure [4-D-glucuronic acid-α1,4-D-N-acetylglucosamine-α1-] n ([-4-D-GlcUA-α1,4-D-GlcNAc-α1-] n ), the method comprising the steps of: culturing a recombinant host cell that produces the polypeptide of SEQ ID NO:1; isolating the polypeptide of SEQ ID NO:1; and contacting the polypeptide of SEQ ID NO:1 with at least one UDP-sugar and a functional acceptor, wherein said UDP-sugar is selected from the group consisting of UDP-glucuronic acid (UDP-GlcUA) and UDP-N-acetyl-glucosamine (UDP-GlcNAc), wherein the functional acceptor is elongated to produce a polymer comprising the structure [-4-D-GlcUA-α1,4-D-GlcNAc-α1-]