Patent ID: 9605306
Date: 2017-03-28
CPC Classifications: C12Q

Claim:
1. A method for detecting methylation of cytosine bases in at least 16 contiguous nucleic acids of SEQ ID NO: 1 from prostate cell genomic DNA from a subject comprising the steps of: a) treating the prostate cell genomic DNA which is derived from a biological sample comprising prostate tumor DNA with bisulfite so that unmethylated cytosine bases are converted to uracil while methylated cytosine bases remain unconverted, wherein the prostate cell genomic DNA comprises a sequence of at least 16 contiguous nucleotides of SEQ ID NO: 1 and the 16 contiguous nucleotides comprise at least one CpG dinucleotide; b) detecting unconverted cytosine bases in the at least 16 contiguous nucleic acids of SEQ ID NO: 1 by amplification of the treated DNA and subsequent hybridization or sequencing; and c) detecting the presence of at least one unconverted cytosine comprised by a CpG dinucleotide in the at least 16 contiguous nucleic acids of SEQ ID NO: 1, wherein the amplification of the treated DNA comprises at least one primer having a length of at least 9 nucleotides and at least one CpG dinucleotide, wherein the primer hybridizes to a nucleic acid sequence selected from the group consisting of SEQ ID NOs: 5, 6, 13 and 14.