Patent ID: 9738911
Date: 2017-08-22
CPC Classifications: C12N,C12P

Claim:
1. A method for biosynthesizing 7-aminoheptanoate in vitro or in a recombinant host cell, said method comprising enzymatically synthesizing a seven carbon chain aliphatic backbone from succinate semialdehyde and pyruvate via aldol condensation, wherein the aldol condensation comprises an aldol reaction catalyzed by a 4-hydroxy-2-oxopimelate aldolase classified under EC 4.1.2.52 and a dehydration catalyzed by a 2-hydroxyhepta-2,4-dienedioate hydratase, and enzymatically forming an amine terminal group in said backbone using a ω-transaminase classified under EC 2.6.1.18, EC 2.6.1.19, EC 2.6.1.29, EC 2.6.1.48, or EC 2.6.1.82, thereby forming the product 7-aminoheptanoate, wherein the host cell is a bacterium, fungus, or yeast.