Patent ID: 9618453
Date: 2017-04-11
CPC Classifications: B82Y,C07F,G01N

Claim:
1. A method of preparing a fluorescent bioprobe comprising the following steps: (i) synthesizing fluorogens with one or more amine- or thiol-reactive functional groups; and (ii) labeling a biomolecule with the fluorogens via a reaction between the fluorogens and one or more amine or thiol groups of the biomolecule; wherein the one or more amine- or thiol-reactive functional groups are selected from the group consisting of isocyanates, isothiocyanates, aldehydes, succinimidyl esters, 4-sulfotetrafluorophenyl esters, 2,4,5,6-tetrafluorophenyl esters, sulfodicholorphenol esters, carbonyl azides, sulfonyl chlorides, and haloacetamides; wherein the fluorescent bioprobe emits fluorescence; wherein the fluorogens exhibit aggregation-induced emission and comprise a backbone structure selected from the group consisting of: wherein: each R and R′ are independently selected from the group consisting of: H, C X is selected from the group consisting of (CH n, m independently=an integer from 0 to 20; and wherein the biomolecule is one or more selected from the group consisting of a nucleotide, nucleoside, oligonucleotide, and DNA, each comprising a reactive thiol or amine group, proteins, and chitosan.