Patent ID: 9683999
Date: 2017-06-20
CPC Classifications: G01N

Claim:
1. A method for detecting an individual who has sarcoidosis or whose blood indicates the presence of sarcoidosis, the method comprising the steps of: a. creating a background test material comprising immune cells from a first aliquot of whole blood; b. creating a microbial catalase-peroxidase protein stimulated test material by contacting immune cells from a second aliquot of whole blood with a first composition; c. creating a purified protein derivative of d. incubating the test materials; e. determining concentrations of interferon-gamma (INFγ) released from each of the test materials; f. evaluating the concentrations of INFγ, defining a positive result if the concentration of INFγ in the microbial catalase-peroxidase protein-stimulated material minus the concentration of INFγ in the background test material is greater than a first threshold level, and the concentration of INFγ in the microbial catalase-peroxidase protein-stimulated test material minus the concentration of INFγ in the PPD-stimulated test material is greater than a second threshold level, wherein the first composition comprises a microbial catalase-peroxidase protein, and the second composition comprises PPD, and wherein the concentration of the microbial catalase-peroxidase protein is ≧0.1 mcg/ml and the concentration of PPD is ≧0.1 mcg/ml.