Patent ID: 9547016
Date: 2017-01-17
CPC Classifications: G01N

Claim:
1. A method for determining the number of polyethylene glycol (PEG) molecules in a polymer:protein conjugate having one or more PEG polymer molecules bound to the protein, the method comprising, contacting the polymer:protein conjugate with a polyclonal antibody that specifically binds the PEG polymer of the polymer:protein conjugate, said polyclonal antibody detectable when bound to the polymer:protein conjugate, and detecting the level of the polyclonal antibody bound to the polymer:protein conjugate, wherein the number of PEG polymer molecules in the polymer:protein conjugate correlates with the level of the polyclonal antibody detected bound to the polymer:protein conjugate when compared to a known control, and wherein polyclonal antibody binding is dose dependent and linear with respect to an amount of bound PEG, and wherein the protein is a blood clotting factor or a blood clotting factor complex.