Patent ID: 9551665
Date: 2017-01-24
CPC Classifications: C12Q,G01N

Claim:
1. A method for detecting mitochondria gene alterations in a microfluidic device, comprising the following steps: (A) providing a microfluidic device, comprising: (B) providing a sample containing mitochondria genes; (C) placing the sample into the first reaction chamber, and introducing the separation element into the first reaction chamber using the first pump, to separate the mitochondria genes from the sample through a binding between the DNA fragment of the separation element and the mitochondria genes in the sample, so as to obtain a detecting sample containing the mitochondria genes; (D) introducing a first portion of the detecting sample into the detecting sample chamber and a second portion of the detecting sample into the comparison chamber using the second pump; (E) adding an amplification solution into both the detecting sample chamber and the comparison chamber and further adding a restriction enzyme into the detecting sample chamber, wherein the amplification solution comprises a labeling reagent and a primer pair, and the restriction enzyme includes ApaI for digesting a target gene region of the mitochondria genes; (F) performing a DNA amplified reaction in both the detecting sample chamber and the comparison chamber, wherein the primer pair is used to amplify the target gene region of the mitochondria genes; (G) determining the relative amount of amplified DNA by measuring a signal from the labeling reagent in the detecting sample chamber and the comparison chamber; and (H) comparing the difference between the signals of the detecting sample chamber and the comparison chamber; wherein when the amount of signal in the detecting sample chamber is less than that in the comparison chamber, it indicates that a mitochondria gene alteration is present in the sample.