Patent ID: 9657344
Date: 2017-05-23
CPC Classifications: C12Q

Claim:
1. A method for obtaining sequence information from a plurality of different target nucleic acids immobilized at discrete locations on a surface, wherein a plurality of the target nucleic acids comprise a common adaptor sequence and have a primer hybridized thereto; wherein the method comprises multiple cycles of the following steps: (a) contacting the target nucleic acids with a solution that contains an enzyme and a detectably labeled nucleotide such that the enzyme catalyzes template-dependent addition of the labeled nucleotide to extend a plurality of the primers, wherein the labeled nucleotide is not a chain-terminating nucleotide, and wherein addition of the labeled nucleotide to a primer interferes with progression of the enzyme; then (b) detecting addition of the labeled nucleotide to at least some of the primers, Thereby obtaining sequence information for at least some of said plurality of different target nucleic acids.