Patent ID: 9725695
Date: 2017-08-08
CPC Classifications: C12N

Claim:
1. A method of maintaining and expanding stem cells in an undifferentiated state in culture comprising culturing the stem cells in a defined low protein culture medium, wherein the amount of protein in said defined low protein medium is about 20× less than in a standard media, which standard media contains 10 g/liter protein, and which low protein culture medium is feeder-free, xeno-free, free of any denaturing agents, and free of animal-derived or human-derived proteins, so as to maintain and expand the stem cells in the undifferentiated state in culture, wherein the medium comprises sodium selenite, an organic acid from the tricarboxylic acid cycle and a combination of growth factors selected from the group consisting of FGF-2 recombinant protein, an IGF-1 recombinant protein or recombinant insulin, a Transferrin recombinant protein, and a TGF beta 1 recombinant protein.