Patent ID: 9540660
Date: 2017-01-10
CPC Classifications: C12N

Claim:
1. A method for generating a stable modified vaccinia Ankara (MVA) virus vector comprising: providing a vector or vectors containing two nucleotide sequences of at least 2000 nucleotides each, wherein each nucleotide sequence encodes a single Ebola virus glycoprotein; substituting at least 400 nucleotides in one or both of the nucleotide sequence(s) to generate two divergent sequences; wherein the 400 substituted nucleotides do not alter the amino acids encoded by the two divergent sequences; and generating a stable MVA vector comprising the two divergent sequences stably inserted into the MVA genome, wherein the two divergent sequences share stretches of identity of no more than 10 contiguous nucleotides; and wherein the two nucleotide sequences encode Ebola virus Sudan and Ebola virus Zaire glycoproteins.