Patent ID: 9556458
Date: 2017-01-31
CPC Classifications: C12N,C12P,C12Y,Y02P

Claim:
1. A genetically modified bacterial host cell that produces methyl ketones, wherein the genetically modified bacterial host cell comprises a recombinant nucleic acid construct encoding a FadM that is capable of converting a β-ketoacyl-CoA to a β-keto acid, and overproduces β-ketoacyl-CoAs compared to a control bacterial host cell that has not been transformed with the nucleic acid construct encoding the FadM, wherein the FadM comprises an amino acid sequence having at least 60% identity to SEQ ID NO:1, and further, wherein the genetically modified host cell: comprises a recombinant nucleic acid sequence that encodes an acyl-CoA oxidase capable of converting an acyl-CoA to a trans-2-enoyl-CoA; comprises a recombinant nucleic acid sequence that encodes a FadB capable of converting a trans-2-enoyl-CoA to a β-hydroxyacyl-CoA and a β-hydroxyacyl-CoA to a β-ketoacyl-CoA; and has an inactive fadA gene or chromosomal deletion of all or part of the fadA gene such that the host cell does not express active FadA.