Patent ID: 9637530
Date: 2017-05-02
CPC Classifications: A61K,A61P,C07K

Claim:
1. A method for preparing an isolated, biologically active recombinant human interleukin-11 (IL-11) cysteine mutein, the method comprising the steps of: (a) obtaining an (b) growing the (c) lysing the cells; (d) separating insoluble proteins from the soluble proteins comprising the IL-11 cysteine mutein fusion protein; (e) isolating the IL-11 cysteine mutein fusion protein from the soluble fraction of the cell lysate obtained from step (d) by chitin affinity column chromatography; wherein the IL-11 cysteine mutein fusion protein binds the chitin affinity column; (f) exposing the IL-11 cysteine mutein fusion protein bound to the chitin affinity column of step (e) to a buffer containing a reducing agent under conditions wherein said reducing agent causes cleavage of the IL-11 cysteine mutein protein from the intein domain; and (g) washing the column with a buffer to collect the cleaved IL-11 cysteine mutein protein; and wherein the IL-11 cysteine mutein comprises: