Patent ID: 9645141
Date: 2017-05-09
CPC Classifications: G01N

Claim:
1. A method for measuring a biomarker, the method comprising: providing magnetic particles having surfaces to which a primary antibody, capable of binding a biomarker is fixed, providing quantum dots having surfaces to which a secondary antibody capable of detecting the biomarker is fixed through a linker protein, reacting the biomarker with the primary antibody fixed to the magnetic particles and the secondary antibody fixed to the quantum dots; separating the magnetic particles from unbound quantum dots by using magnetic forces; selectively separating only quantum dots bound to the magnetic particles by adding ethylenediamine tetraacetic acid (EDTA) to break a nickel-histidine binding reaction; and quantifying the concentration of the biomarker by measuring absorbance or intensity of fluorescence of the selectively separated quantum dots.