Patent ID: 9610333
Date: 2017-04-04
CPC Classifications: A61K,A61P,C07K,C12N,Y02A

Claim:
1. A method of using a heat stable vaccine preparation to immunize a subject to an infectious agent, a tumor, or an allergen, the method comprising: providing a a vegetative promoter for cytoplasmic vegetative expression of the antigen in the vegetative cells, or a sporulation promoter for expression of the antigen as a fusion to a spore coat protein on the collecting the cells or the spores from the culture by centrifugation and heat-stabilizing the antigen by lyophilizing the vegetative cells or the spores to obtain a heat stable vaccine preparation having heat stable immunogenic potency for at least 12 months at a temperature of 45° C. comparable to the vaccine stored at 4° C.; and, contacting a mucosal tissue of the subject with the heat stable vaccine preparation which induces an immune response and immunizes the subject to the infectious agent, the tumor, or to the allergen.