Patent ID: 9541480
Date: 2017-01-10
CPC Classifications: C07K,G01N

Claim:
1. A method comprising: (a) contacting a biological sample comprising rare cells and non-specific cells to a non-fouling lipid layer of a microfluidic device, the microfluidic device comprising an antibody which selectively binds to rare cells, and wherein the non-fouling lipid layer is non-covalently associated with the antibody; (b) binding the rare cells to the antibody; (c) washing the non-fouling lipid layer with a liquid, wherein the non-specific cells in contact with the non-fouling lipid layer are released from the non-fouling lipid layer; and (d) flowing a liquid comprising bubbles through a channel of the microfluidic device of step (c), thereby releasing the rare cells from the microfluidic device.