Patent ID: 9579261
Date: 2017-02-28
CPC Classifications: A61J,A61K,A61P,B01D

Claim:
1. A method for preparing liposome formulation, the method comprising the steps of: (a) dissolving a lipid fraction comprising at least one kind selected from phospholipid and sterol, and a bioactive substance in an organic solvent; (b) putting an organic solvent solution of the step (a) together with a saccharide carrier into a reaction vessel and preparing proliposome particles coated with a lipid-bioactive substance mixture by introducing a supercritical fluid thereto, wherein the supercritical fluid and the organic solvent solution of step (a) are mixed together until reaching equilibrium, wherein, after reaching equilibrium, an additional supercritical fluid is added, the saccharide carrier comprising one or more components selected from the group consisting of lactose, sucrose, maltose, trehalose, dextrose, sorbitol, mannitol, and xylitol; (c) discharging the supercritical fluid in step (b) by decompression so as to obtain the proliposome particles; and (d) hydrating the proliposome particles in step (c) by an aqueous solution comprising water at a temperature in the range of 65 to 95° C. so as to form a liposome solution, wherein the organic solvent including the lipid fraction is divided into a bioactive-component dissoluble solvent and lipid-fraction dissoluble solvent and the bioactive-component dissoluble solvent is at least one kind selected from the group consisting of dimethylacetamide, dimethylformamide and dimethylsulfoxide, and wherein the bioactive substance is amphotericin B.