Patent ID: 9701936
Date: 2017-07-11
CPC Classifications: A61K,C07K,C12M,C12N,C12P

Claim:
1. A process for culturing a fastidious bacterial strain in a fermenter comprising the steps of: a) adding a liquid growth medium comprising a carbon source to a fermenter; b) seeding the growth medium with the fastidious bacteria to form a culture medium; and c) growing the fastidious bacteria in perfusion suspension culture, where an average perfusion rate is above 5% of culture volume per hour; wherein the culture medium including the bacteria is circulated over a separation system in alternating tangential flow, and the separation system removes a filtrate containing spent medium from the culture medium and retains the fastidious bacteria in the culture medium, d) reducing the perfusion rate of step c) when the carbon source in the medium is exhausted.