Patent ID: 9557336
Date: 2017-01-31
CPC Classifications: A61K,C07C,C07D,C07K,C09B,C12N,C12Y,G01N

Claim:
1. A method for forming a covalent linkage between a polypeptide and a chemical species, the method comprising the steps of: a) providing a polypeptide, wherein the polypeptide comprises a thioester group and/or wherein the polypeptide is C-terminally fused to an intein; b) providing a chemical reagent of formula (I): or a salt of the chemical reagent, wherein: i) R R is —ONH L is a single bond; ii) R R is —ONH L is a linker or linker group of formula iii) R R is a fluorescent molecule selected from the group consisting of a coumarin derivative, a naphthalene derivative, a pyrene derivative, a fluorescein derivative, a rhodamine derivative, a naphthoxanthene derivative, a phenanthridine derivative, a boron difluoride dipyrromethene (BODIPY) derivative, a cyanine derivatives, a phthalocyanine derivative, and an oxazine derivative, and L is —C(O)NH(CH) iv) R R is biotin or a biotin analogue, and L is —C(O)NH(CH v) R R is a poly(ethyleneglycol) molecule, and L is —C(O)NH(CH the chemical reagent being reactive with the polypeptide, and wherein reaction of the chemical reagent with the polypeptide forms a covalent linkage between the chemical reagent and the polypeptide; and c) allowing the polypeptide to react with the chemical reagent so that a covalent linkage between the reagent and the polypeptide is formed.