Patent ID: 9706724
Date: 2017-07-18
CPC Classifications: A23L,A23V,C12N

Claim:
1. A plant comprising an expression cassette for reducing gene expression of a plant target gene sequence, comprising: (i) a polynucleotide that shares sequence identity with at least a part of the plant target gene sequence; and (ii) an inverted repeat of the polynucleotide, wherein the polynucleotide and its inverted repeat are positioned in between, and operably linked to, two promoters that are each functional in plants, wherein (a) the polynucleotide and its inverted repeat are fully identical in sequence over at least 23 nucleotides, (b) the direction of transcription is from one promoter toward the other promoter, and (c) neither the polynucleotide nor its inverted repeat is operably linked to a terminator.