Patent ID: 9637763
Date: 2017-05-02
CPC Classifications: C12N,C12P

Claim:
1. A modified recombinant host cell for producing a phenolic compound, comprising i) a first expression system that comprises at least one nucleotide sequence that encodes a 6-methylsalicylic acid synthase (6-MSAS) or an orsellinic acid synthase (OSAS), capable of being expressed, and ii) a second expression system that comprises at least one nucleotide sequence that encodes a holo acyl carrier protein (ACP) synthase, wherein the ACP synthase pantetheinylates said 6-MSAS or OSAS, and iii) a third expression system that comprises at least one nucleotide sequence that encodes a functional decarboxylase which catalyzes decarboxylation to form said phenolic compound, such that the product isolated from said cell is a phenolic compound, wherein either: the first expression system for 6-MSAS or OSAS and the second expression system for ACP synthase are present on a single vector or on separate vectors, and wherein the third expression system for a functional decarboxylase is present on the same vector as the 6-MSAS or OSAS, or the holo ACP synthase, or both, or is present on a separate vector, or; wherein the first, second or third expression systems are integrated into the host cell chromosome or expressed from yeast artificial chromosomes (YACs).