Patent ID: 9539271
Date: 2017-01-10
CPC Classifications: A61K,A61P,C08B

Claim:
1. A depolymerized holothurian glycosaminoglycan (DHG) that is used for the preparation of a drug for prevention or treatment of thromboembolic diseases, wherein the drug comprises a depolymerized holothurian glycosaminoglycan and a pharmaceutically acceptable carrier, wherein the depolymerized holothurian glycosaminoglycan has a weight-average molecular weight within the range of from about 26,000 Da and 45,000 Da, wherein the depolymerized holothurian glycosaminoglycan is prepared via a process that comprises the steps as follows: (1) mincing a holothurian, wherein the holothurian is Holothuria leucospilota, (2) adding an enzyme into the minced holothurian, wherein the enzyme comprises a proteolytic enzyme and a compound pancreatin, an amount of the proteolytic enzyme is 2% of a weight of the holothurian, and an amount of the compound pancreatin is 2% of the weight of the holothurian; (3) performing an enzymatic hydrolysis and precipitation on the product of step (2) to form a holothurian glycosaminoglycan, (4) collecting a crude product of holothurian glycosaminoglycan, (5) purifying and decolorizing the crude product of holothurian glycosaminoglycan, and (6) collecting the holothurian glycosaminoglycan; (7) adding hydrogen peroxide with a weight concentration of about 5% to about 10% into a product obtained in the step (6) for degradation, (8) collecting a depolymerized holothurian glycosaminoglycan with a weight average molecular weight within a range of from 26,000 Da to 45,000 Da.