Patent ID: 9663813
Date: 2017-05-30
CPC Classifications: C12M,C12N,C12Q

Claim:
1. A method for filtering a sample to selectively retain micro-organisms onto a filter, the method comprising: (a) providing a sample with eukaryotic cells and containing or suspected to contain a micro-organism; (b) performing a selective lysis of the eukaryotic cells to obtain a lysed sample; (c) filtering a portion of the lysed sample obtained in step (b) through a filter arranged to retain the micro-organism; (d) washing the filter with a detergent-based wash buffer having a detergent concentration that selectively solubilizes substances other than the micro-organism retained by the filter, said washing includes passing the detergent-based wash buffer through the filter, to selectively remove clogs consisting of the substances other than the micro-organism from the filter; and (e) repeating step (c) at least one additional time, to filter at least one additional portion of the lysed sample, wherein the detergent concentration in the detergent-based wash buffer is between about 0.2% and about 0.5% by volume.