Patent ID: 9683262
Date: 2017-06-20
CPC Classifications: C07K,C12N,C12Q,G01N

Claim:
1. A method of isolating a subset of CD8 T cells that produce interleukin-13 upon activation, the method comprising the steps of: obtaining a sample of lymphocytes from a mammal; activating the sample of lymphocytes with immune-irradiated splenocytes to obtain a polyclonal T cell clone population, wherein the immune-irradiated splenocytes present an antigen recognized by the sample of lymphocytes; expanding the polyclonal T cell clone population; selectively depleting the CD4 T cell clones present in the polyclonal T cell clone population to isolate an activated CD8 T cell clone population; measuring gene expression in the activated CD8 T cell clone population; determining if any of the activated CD8 T cell clone population expresses an elevated level of a biomarker as compared to a healthy control, the biomarker selected from a group consisting of: 1810011H11Rik and C10orf128; and isolating a subset of CD8 T cell clones that express the elevated level of the biomarker from the activated CD8 T cell population; wherein the elevated level of the biomarker is indicative of interleukin-13 production by the subset of CD8 T cells.