Patent ID: 9672324
Date: 2017-06-06
CPC Classifications: C12N,C40B,G16B

Claim:
1. A method of forming a metadatabase of minimal epitope profiles for use in a diagnostic, the method comprising: a) providing a plurality of biological samples; b) performing antibody activity profiling of an immunoglobulin fraction of the samples to form a data set of peptide profiles for each sample, wherein the peptide profiles comprise antigen-antibody interaction sequences, wherein the antibody activity profiling comprises the steps of: c) comparing peptide profiles across different samples to generate a meta-profile defined by an amino acid sequence of the motif and its frequency pattern; d) assigning a diagnosis or disorder to differentially expressed meta-profiles generated from samples affected by the diagnosis or disorder; e) constructing a meta-database containing minimal epitope clusters (motif_summary), defined according to the peptide frequency patterns over multiple samples; f) constructing a database of peptide frequencies (pep_summary), where frequency of occurrence of each of the peptide antigen is recorded for all samples; g) aligning peptides of a characterized sample to reveal similarity in amino acid sequence thereby defining the sequence of the minimal epitope; h) verifying the minimal epitope by harboring a connected identifier—proteinaceous antigen compound wherein the identifier is a molecule or substance allowing later identification of the respective peptide identity; i) providing an enriched affinity sample for minimal epitope defining sequence generation; j) providing a source data for metadatabase and any database underlying it by any amplification and sequence identification method; and k) generating a peptide comprising the minimal epitope as a diagnostic for the diagnosis or disorder.