Patent ID: 9574242
Date: 2017-02-21
CPC Classifications: C12Q

Claim:
1. Method for detection of a methicillin resistant coagulase positive Staphylococcus aureus (MRSA) strain, said MRSA strain comprising a chromosomal Staphylococcus mec (SSCmec) cassette, the method comprising performing a sequence specific amplification reaction with one or more first forward primer(s) comprising a sequence according to SEQ ID NO: 1 (CATCATTTATGGTATGCTT CTCCAC), wherein 5′ end of the one or more first forward primer(s) hybridizes to a target DNA sequence within the SSCmec cassette and a 3′ end of the one or more first forward primer(s) hybridizes in an adjacent chromosomal DNA wherein a melting temperature (Tm) of the one or more first forward primer(s) is between 55° and 65° C.