Patent ID: 9617585
Date: 2017-04-11
CPC Classifications: B01J,C07B,C07H,C12N,C12Q,C40B,G01N

Claim:
1. A process for detecting or quantifying more than one nucleic acid of interest in a library comprising the steps of: a) providing b) contacting said nucleic acid analytes with said first set of primers to form a first bound entity comprising first set primer hybridized to a nucleic acid analyte of the library; c) using the polymerizing means, extending the primer of said first bound entity using the nucleic acid analyte of the first bound entity as a template sequence to form first nucleic acid copies of said analytes; d) using the addition means, extending said first nucleic acid copies by the addition of non-template derived sequences to the 3′ end of said first nucleic acid copies to form extended first nucleic acid copies; e) contacting said extended first nucleic acid copies with said second set of primers to form a second bound entity comprising second set primer hybridized to the extended first nucleic acid copies; f) using the polymerizing means, extending the primer of said second bound entity using the extended first nucleic acid copies of the second bound entity as a template sequence to form second nucleic acid copies; g) separating said second nucleic acid copies from the said extended first nucleic acid copies; h) repeating steps e), f) and g) until a desirable amount of second nucleic acid copies have been synthesized; i) hybridizing said second nucleic acid copies formed in step h) to said array of nucleic acids provided in step (a)(i); and j) detecting or quantifying any of said hybridized second nucleic acid copies obtained in step i).