Patent ID: 9617563
Date: 2017-04-11
CPC Classifications: C12N,C12P,C12Y

Claim:
1. A recombinant Escherichia coli ( E. coli ) or Pichia yeast cell capable of producing β-phellandrene, prepared by introducing at least one nucleic acid selected from the group consisting of a nucleic acid encoding geranyl pyrophosphate synthase and a nucleic acid encoding neryl pyrophosphate synthase, and a nucleic acid encoding β-phellandrene synthase into a host cell in such a manner that these nucleic acids are expressed in the host cell, wherein the nucleic acid encoding geranyl pyrophosphate synthase encodes the following protein (a), (b) or (c): (a) protein consisting of the amino acid sequence of SEQ ID NO:2, (b) protein consisting of the amino acid sequence of SEQ ID NO:2 in which 1 to 20 amino acids are deleted, substituted or added in the amino acid sequence, and having geranyl pyrophosphate synthase activity, or (c) protein consisting of an amino acid sequence having a homology of 90% or more with SEQ ID NO:2, and having geranyl pyrophosphate synthase activity, wherein the nucleic acid encoding neryl pyrophosphate synthase encodes the following protein (d), (e) or (f): (d) protein consisting of the amino acid sequence of SEQ ID NO:4, (e) protein consisting of the amino acid sequence of SEQ ID NO:4 in which 1 to 20 amino acids are deleted, substituted or added in the amino acid sequence, and having neryl pyrophosphate synthase activity, or (f) protein consisting of an amino acid sequence having a homology of 90% or more with SEQ ID NO:4, and having neryl pyrophosphate synthase activity, and wherein the nucleic acid encoding β-phellandrene synthase encodes the following protein (g), (h) or (i): (g) protein consisting of the amino acid sequence of SEQ ID NO:6 or 8, (h) protein consisting of the amino acid sequence of SEQ ID NO:6 or 8 in which 1 to 20 amino acids are deleted, substituted or added in the amino acid sequence, and having β-phellandrene synthase activity, or (i) protein consisting of an amino acid sequence having a homology of 90% or more with SEQ ID NO:6 or 8, and having β-phellandrene synthase activity, and wherein 10 mg or more of β-phellandrene can be produced per 1 g of wet cells of the recombinant cell.