Patent ID: 9593311
Date: 2017-03-14
CPC Classifications: A61K,A61P,C12N

Claim:
1. A method of reprogramming a mammalian somatic cell, comprising: (a) introducing into the mammalian somatic cell, one or more retroviral vectors encoding Oct4, and optionally encoding one or more reprogramming factors selected from the group consisting of: Klf4 and Sox2, wherein c-Myc is not introduced into the cell; (b) contacting the mammalian somatic cell with a Wnt-3a conditioned medium; or an agent that modulates the Wnt pathway selected from the group consisting of a recombinant, exogenous, soluble, and biologically active Wnt protein, and a GSK-3 antagonist; and (c) culturing the cells under conditions suitable for reprogramming the mammalian somatic cell to a pluripotent state.