Patent ID: 9551705
Date: 2017-01-24
CPC Classifications: G01N

Claim:
1. A method of capturing a cellular target comprising the steps of: (a) administering to a cell, a conjugate comprising a small molecule bioactive agent tethered by a carbamate linker to a chloroalkane capture ligand, the cell comprising a cellular target of the small molecule bioactive agent, under conditions such that the cellular target non-covalently binds the small molecule bioactive agent; (b) lysing the cell to produce a cell lysate; (c) contacting the cell lysate with a solid surface displaying a capture protein comprising a dehalogenase modified to covalently bind to the chloroalkane capture ligand under conditions in which the capture protein forms a covalent bond with the chloroalkane capture ligand; and (d) separating the solid surface with the bound cellular target from the cell lysate.