Patent ID: 9624467
Date: 2017-04-18
CPC Classifications: A01N,A61K,C12N

Claim:
1. A method for large scale preparation of a biocontrol formulation comprising a high concentration of viable Streptomyces spp. spores, the method comprising: culturing a Streptomyces bacterium selected from the group consisting of collecting spores from the Streptomyces bacterium; culturing the spores to obtain a seed inoculum; and amplifying the seed inoculum in broth medium in a large-scale serial fermentor at about 28 to 37° C., 80 to 250 rpm, 0.25 to 0.75 vvm and pH at 5.0 to 8.0 to directly yield a biocontrol formulation comprising the broth medium containing at least 10 wherein the broth medium is modified Czapek's culture medium containing 1% (w/v) to 3% (w/v) oat, wheat, or corn, optionally 0.12-0. 6% (w/v) pectin, 0.15% (w/v)or 1% (w/v) chitin, and 0 to 1.0% (w/v) malt extract.