Patent ID: 9709566
Date: 2017-07-18
CPC Classifications: B01D,G01N

Claim:
1. A chromatography method comprising: (a) developing a complex of a test substance and a labeling substance modified with a first binding substance which specifically binds to the test substance, onto an insoluble carrier; (b) causing the complex to be trapped in a reaction site on the insoluble carrier, wherein the reaction site contains a second binding substance which specifically binds to the test substance or contains a substance which specifically binds to the first binding substance; (c) washing the insoluble carrier with a washing solution containing potassium iodide after the step (b), wherein the concentration of potassium iodide in the washing solution ranges from 50 mmol/L to 800 mmol/L and the washing solution has a pH of 5-8; (d) washing off the washing solution remaining on the insoluble carrier from the insoluble carrier; and (e) amplifying the labeling substance of the complex trapped in the reaction site, wherein in the step (d), a first amplification solution containing a reductant capable of reducing silver ion is used to wash off the washing solution from the insoluble carrier, and in the step (e), a second amplification solution containing a silver ion-containing compound is used to amplify the labeling substance of the complex.