Patent ID: 9745617
Date: 2017-08-29
CPC Classifications: C12Q

Claim:
1. A method of performing a digital assay, comprising: amplifying at least two distinct targets in droplets containing a reporter for each distinct target, wherein the reporters each are labeled with only a same luminophore; detecting a signal from the droplets representing combined emission of light in a same wavelength from the reporters for the distinct targets, wherein each reporter provides a portion of the signal having an intensity that varies according to whether the corresponding distinct target is present in a droplet, and wherein a plurality of the droplets each contain at least one of the distinct targets and have a distinguishable intensity of the signal relative to droplets containing only one of the distinct targets; determining for each distinct target a number of droplets that are positive for only one of the distinct targets; determining at least one number of droplets that are positive for each of the distinct targets; and determining a quantity representative of a level of at least one of the distinct targets.