Patent ID: 9687152
Date: 2017-06-27
CPC Classifications: A61B,A61N,G01J,G02B

Claim:
1. A microscope comprising: an excitation source operable to emit an optical excitation beam at an excitation wavelength λ; a scanner operable to scan the excitation beam on a sample; an objective arranged to receive the excitation beam output by the scanner, irradiate the sample with the excitation beam scanned by the scanner and collect an emission beam from the sample; a first detector operable to detect one or more multiphoton signals in the emission beam, the multiphoton signals arising from multiphoton processes in the sample and a second detector operable to detect a confocal reflectance signal in the emission beam; an emission light path comprising a first wavelength separator operable to separate the emission beam into a first part comprising the one or more multiphoton signals and a second part comprising the confocal reflectance signal, the first wavelength separator allowing transmission from the objective to the first detector of a wavelength band limited to greater than or equal to λ/2 and less than λ, wherein the one or more multiphoton signals have wavelengths within the wavelength band; wherein the one or more multiphoton signals comprises a first multiphoton signal and a second multiphoton signal of different types and the confocal reflectance signal detected at the second detector is coregistered with the one or more multiphoton signals detected at the first detector.