Patent ID: 9637756
Date: 2017-05-02
CPC Classifications: A23K,A23L,A23V,C07K,C12N,C12Y,Y02A

Claim:
1. A method of increasing tolerance to salinity in a plant, said method comprising: providing a plurality of plants comprising a promoter operably linked to a nucleic acid, wherein the promoter and the nucleic acid are heterologous to each other and whereby the nucleic acid is expressed, and said nucleic acid comprising a nucleotide sequence selected from the group consisting of: a) a nucleotide sequence that encodes a protein comprising the amino acid sequence of SEQ ID NO: 154 and having the sulfotransferase activity of SEQ ID NO: 154, b) the nucleotide sequence according to SEQ ID NO: 153 and encoding the sulfotransferase protein of SEQ ID NO: 154, and c) a nucleotide sequence hybridizing to the full length complementary sequence of the nucleotide sequence as set forth in SEQ ID NO: 153 under high stringency conditions which comprise (i) hybridization at 42° C. in 50% formamide, 6×SSC or 6×SSPE, 0.05% Blotto or 5×Denhardt's Reagent, 100 μg/ml denatured salmon sperm DNA, 0.05% SDS and washing at 65° C. first in 2×SSC, 0.1% SDS for at least 30 min to one hour and subsequently in 0.1×SSC, 0.5% SDS for at least 30 min to one hour; or (ii) hybridization at 65° C. in 6×SSC or 6×SSPE, 0.05% Blotto or 5×Denhardt's Reagent, 100 μg/ml denatured salmon sperm DNA, 0.05% SDS and washing at 65° C. first in 2×SSC, 0.1% SDS for at least 30 min to one hour, and subsequently in 0.1×SSC, 0.5% SDS for at least 30 min to one hour, wherein said hybridizing nucleotide sequence encodes a protein having the sulfotransferase activity of SEQ ID NO: 154; and selecting from the plurality of plants a plant exhibiting increased tolerance to salinity as compared to a control plant.