Patent ID: 9597346
Date: 2017-03-21
CPC Classifications: A61K,A61P,C07K,C12N

Claim:
1. A method of reducing level of at least one target protein in a cell comprising the step of contacting the cell with a first agent and a second agent, wherein the first agent is an RNA interference (RNAi) molecule which reduces synthesis of the target protein, wherein the second agent is a chimeric polypeptide comprising a ubiquitin ligase polypeptide and a target protein interacting domain, wherein the ubiquitin ligase polypeptide is β-TrCP, wherein the target protein interacting domain is a polypeptide derived from papillomavirus E7, wherein the target protein is naturally-occurring or a mutant of Rb, p107, p130 or a combination thereof, wherein the first agent contacts the cell before, after or simultaneously with the second agent, and wherein the level of the target protein is reduced by greater than 90%.