Patent ID: 9547750
Date: 2017-01-17
CPC Classifications: B82Y,C12Q,G06N,G16B

Claim:
1. A nucleic acid-based molecular computing system, comprising: (i) an input nucleic acid strand comprising a first fragment (A) and a second fragment (B); (ii) a first target, incompletely base-paired nucleic acid duplex comprising a first strand and a second strand, (iii) a second target, incompletely base-paired nucleic acid duplex comprising a first strand and a second strand, (iii) a third target, incompletely base-paired nucleic acid duplex comprising a first strand and a second strand, wherein the first fragment (B) of the second strand of the first target duplex comprises a first chemical modification, relative to a natural nucleic acid, that causes binding with the second fragment (B′) of the first strand of the second target duplex to have a decreased hybridization free energy and/or the second fragment (B) of the input strand comprises a second chemical modification, relative to a natural nucleic acid, that causes binding with the second fragment (B′) of the first strand of the second target duplex to have an increased hybridization free energy, such that the first fragment (B) of the second strand of the first target duplex, as compared to the second fragment (B) of the input strand, binds to the second fragment (B′) of the first strand of the second target duplex to form a more stable duplex region; and wherein the first fragment (C) of the second strand of the second target duplex comprises a third chemical modification, relative to a natural nucleic acid, that causes binding with the second fragment (C′) of the first strand of the third target duplex to have a decreased hybridization free energy and/or the second fragment (C) of the second strand of the first target duplex comprises a fourth chemical modification, relative to a natural nucleic acid, that causes binding with the second fragment (C′) of the first strand of the third target duplex to have an increased hybridization free energy, such that the first fragment (C) of the second strand of the second target duplex, as compared to the second fragment (C) of the second strand of the first target duplex, binds to the second fragment (C′) of the first strand of the third target duplex to form a more stable duplex region.