Patent ID: 9625472
Date: 2017-04-18
CPC Classifications: C12M,G01N

Claim:
1. A method of performing a cell-based assay, comprising: (a) providing a cell-substrate impedance monitoring system, comprising: (b) a software program capable of controlling the device station and performing data acquisition and data analysis from said impedance analyzer; (c) introducing cells characterized as a having a cancer lineage into one or more wells of said device, wherein at least one of said one or more wells comprises an electrode array; (d) culturing said cells to achieve an exponential growth phase; (e) monitoring cell-substrate impedance of said at least one well; (f) adding a drug suspected of being having an anti-cancer activity to said cells while in the exponential growth phase; (g) continuing to monitor cell-substrate impedance of said at least one well; and (h) assaying cell status, wherein cell status refers to one or more selected from the group consisting of cell attachment or adhesion on the substrate including on the electrodes, the degree of cell spreading, the attachment area of the cell, the degree of tightness of cell attachment, cell morphology, cell growth or proliferation status; number of viable cells and/or dead cells in a well, cytoskeleton change and re-organization, and number of cells going through apoptosis and/or necrosis.