Patent ID: 9683985
Date: 2017-06-20
CPC Classifications: A61P,C07K,G01N

Claim:
1. An in vitro method for identifying/selecting a modified therapeutic protein that treats tumors and that has greater activity in a tumor microenvironment in a subject compared to a non-tumor environment in the subject, comprising: 1) testing the binding activity of a plurality of modified therapeutic anti-tumor proteins to the target of the therapeutic anti-tumor proteins under conditions a) and under conditions b), wherein: 2) comparing the binding activity of the modified therapeutic proteins in a) to the binding activity in b); and 3) selecting/identifying a modified therapeutic protein that has greater binding activity for the target protein in a) compared to b), thereby identifying a modified therapeutic antibody or antigen-binding portion thereof that is conditionally active such that it has greater binding activity in the low pH conditions of a tumor microenvironment compared to the pH conditions of a non-tumor environment.