Patent ID: 9605303
Date: 2017-03-28
CPC Classifications: C12Q

Claim:
1. A method for genotyping a target nucleic acid in a sample is provided comprising: (a) generating a first detection mixture by a method comprising contacting a portion of the sample with a first probe set, wherein the first probe set comprises a nucleic acid probe specific for a first genotype of the target nucleic acid and a nucleic acid probe specific for a second genotype of the target nucleic acid, but does not comprise a nucleic acid probe specific for a third genotype of the target nucleic acid; (b) generating a second detection mixture by a method comprising contacting a portion of the sample with a second probe set, wherein the second probe set comprises a nucleic acid probe specific for the second genotype of the target nucleic acid and a nucleic acid probe specific for the third genotype of the target nucleic acid, but does not comprise a nucleic acid probe specific for the first genotype of the target nucleic acid; and (c) treating the first and second detection mixtures under conditions wherein the nucleic acid probes hybridize specifically to the first, second, and/or third genotype of the target nucleic acid; and (d) detecting hybridization of the nucleic acid probe to the target nucleic acid, wherein: