Patent ID: 9657322
Date: 2017-05-23
CPC Classifications: A61P,C12N,C12P,C12Y

Claim:
1. A method for producing a mixture of gluco-oligosaccharides having one or more consecutive (α1→6) glucosidic linkages and one or more consecutive (α1→4) glucosidic linkages, comprising contacting a poly- and/or oligosaccharide substrate comprising at its non-reducing end at least two α1→4-linked D-glucose units with an α-glucanotransferase enzyme capable of cleaving (α1→4) glucosidic linkages and making new (α1→4) and (α1→6) glucosidic linkages, wherein said α-glucanotransferase enzyme comprises a polypeptide sequence having at least 60% identity to the catalytic core of α-glucanotransferase enzyme GTFB from Lactobacillus reuteri 121(SEQ ID NO. 59), wherein the α-glucanotransferase does not introduce (α1→6) branching points.