Patent ID: 9623052
Date: 2017-04-18
CPC Classifications: A61K,A61L,A61P,C12N

Claim:
1. A method for producing a myocardial sheet, comprising the following steps (a) to (c): (a) producing Flk/DR positive cells, cardiomyocytes, endothelial cells, and mural cells from embryonic stem cells; and (b) mixing the Flk/KDR positive cells with the cardiomyocytes, endothelial cells, and mural cells to obtain a cell mixture of FLk/KDR positive cells, cardiomyocytes, endothelial cells, and mural cells; and (c) forming a sheet by culturing the mixed cells obtained in step (b) in a culture medium comprising VEGF in a culture vessel coated with a temperature-responsive polymer.