Patent ID: 9581798
Date: 2017-02-28
CPC Classifications: G02B

Claim:
1. A light sheet microscope for recording 3D images of a specimen, comprising: a light sheet generator and sheet scanning unit that produces a light sheet that is scanned through the specimen, an objective lens positioned to receive the signal generated by the scanned light sheets and an image detector positioned to receive light from the objective lens, a wavefront encoder (WFE) element or system positioned between the objective lens and the image detector at an exit pupil plane or a conjugated plane of the objective lens, wherein the WFE extends the depth of field of the objective lens, and wherein the WFE is selected to generate an optical path difference resulting in a phase modulation that permits creating a 3D image from 2D images generated by scanning the light sheet with the scanning unit while the objective lens and the specimen are static.