Patent ID: 9702017
Date: 2017-07-11
CPC Classifications: C12Q

Claim:
1. A method for simultaneously detecting the presence or absence of genotypes 1, 2, 3, and/or 4 of hepatitis E virus (HEV) in a biological sample, comprising the steps of: (a) isolating nucleic acids present in the biological sample; (b) amplifying the nucleic acids isolated in step (a) in a single reaction mixture, using one non-degenerate forward primer comprising SEQ ID NO: 6 and at least one non-degenerate reverse primer comprising a sequence selected from the group consisting of SEQ ID NOS: 11, 13, and 14, wherein the forward and reverse primers simultaneously amplify genotypes 1, 2, 3, and/or 4 of HEV, if present in the biological sample; and (c) detecting the presence or absence of amplified nucleic acid obtained in step (b), wherein the presence of amplified nucleic acid obtained in step (b) is an indication of the presence of genotypes 1, 2, 3, and/or 4 of HEV in the biological sample, and wherein the absence of amplified nucleic acid obtained in step (b) is an indication of the absence of genotypes 1, 2, 3, and/or 4 of HEV in the biological sample.