Patent ID: 9663823
Date: 2017-05-30
CPC Classifications: C12N,C12Q,G01N

Claim:
1. A method for determining whether a test compound activates an NFkB-mediated response in a cell comprising: a) exposing a plurality of no more than 100 collections of cells to a test compound, wherein the cells in each collection comprise an expression construct comprising a promoter operatively linked with a heterologous sequence encoding a reporter, wherein the promoter within each collection is the same and each collection has a different promoter, wherein a plurality of the promoters are NFkB biomarker promoters of different genes and are selected from: (1) SEQ ID NO: 139 (IRF1); SEQ ID NO: 140 (TNFA1P3); SEQ ID NO: 142 (RELB); SEQ ID NO: 144 (IL8); SEQ ID NO: 145 (VCAM1); SEQ ID NO: 149 (CD40); SEQ ID NO: 151 (CSF1); and SEQ ID NO: 153 (NFKB2); (2) a sequence of at least 500 nucleotides having at least 98% sequence identity to a sequence of (1); and (3) a fragment of at least 500 nucleotides of a sequence of (1); b) measuring expression of the reporter; and c) correlating the measurement with an NFkB-mediated response, wherein increased expression of the reporter compared to a control, indicates that the test compound induces an NFkB-mediated response and decreased expression of the reporter compared with a control indicates that the test compound inhibits an NFkB-mediated response.