Patent ID: 9598687
Date: 2017-03-21
CPC Classifications: C07K,C12N

Claim:
1. A method for purifying a recombinant coagulation factor protein, the method comprising: a) providing a mixture comprising the recombinant coagulation factor protein; b) filtering the mixture through at least a first anion exchange column to produce a first anion exchange column product; c) loading the first anion exchange column product onto a poly(ethyleneimine) (PEI) column and eluting with a linear sodium chloride gradient to produce one or more fractions of a PEI column product; and d) recovering one or more fractions of PEI column product with a combined pre-peak of less than 30% to obtain the purified recombinant coagulation factor protein, wherein the purified recombinant coagulation factor protein comprises less than about 0.8/10, 0.7/10, 0.6/10, 0.5/10 or 0.4/10 missing D-carboxylated glutamic acid sites and wherein the pre-peak of one or more PEI column product is determined by high performance liquid chromatography (HPLC).