Patent ID: 9745611
Date: 2017-08-29
CPC Classifications: C12Q

Claim:
1. A composition comprising a mixture of a forward primer set and a reverse primer set for identifying a plurality of microorganisms in a sample, wherein each primer set comprises one or more oligonucleotide primers, and at least one set comprises multiple primers; each primer comprises a domain-specific sequence that is substantially complementary to a conserved domain of a bacterial or archaeal ribosomal 16S DNA sequence adjacent to the V3, V4, or V5 region of said 16S DNA sequence; and for each primer set comprising multiple primers, the primers within the set are overlapping primers which are substantially complementary to the same conserved domain, and each primer within the set differs from at least another primer within the set by a frame shift of one to three nucleotides at the 5′ end of the domain-specific sequence, such that said forward primer set and said reverse primer set generate frame-shifted amplicons in a polymerase-chain reaction (PCR), and wherein each primer in said composition further comprises an at least partial adapter sequence for facilitating sequencing of said sequence.