Patent ID: 9597358
Date: 2017-03-21
CPC Classifications: A61F,A61K,A61L,C12N

Claim:
1. A method of preparing an aligned extracellular matrix (ECM) scaffold comprising culturing functional keratocytes on a template scaffold comprising one or more layers comprising aligned fibers of a biocompatible, biodegradable polymeric composition for a length of time sufficient for the cells to produce a product ECM scaffold having oriented fibers, wherein the biocompatible, biodegradable polymeric composition comprises: a polyurethane, a polyester, a polyether, a polyacrylamide, and/or a polycarbonate; a polyurethane, a polyester, a polyether, a polyacrylamide, and/or a polycarbonate-containing block copolymer, or a copolymer formed from one or more acrylic monomers, acrylamide monomers, succinimide monomers, glycolide monomers, caprolactone monomers, dioxanone monomers, lactide monomers, and/or carbonate monomers, and wherein the template scaffold degrades so that the product ECM scaffold does not contain the biocompatible, biodegradable polymeric composition of the template scaffold.