Patent ID: 9707276
Date: 2017-07-18
CPC Classifications: A61K,A61P

Claim:
1. A composition comprising a conjugate comprising: An O-glycosylated insulin or insulin analogue having an A-chain peptide comprising the amino acid sequence GIVEQCCTSICSLYQLENYC (SEQ ID NO: 90); a B-chain peptide comprising the amino acid sequence HLCGSHLVEALYLVCGERGFF (SEQ ID NO:3), and a carboxy terminal portion (CTP) peptide comprising amino acids 112-118 to 145 of the beta subunit of human chorionic gonadotropin (hCGβ) that includes at least one O-glycosylation site of the CTP peptide, wherein at least one amino acid residue of the CTP peptide is covalently linked to an O-glycan wherein the O-glycan in the composition is a mannose, mannobiose, mannotriose, or mannotetrose; and wherein the insulin or insulin analogue optionally further includes up to 17 amino acid substitutions and the CTP is covalently linked by peptide bond to the N-terminus of the B-chain, the C-terminus of the A-chain, the C-terminus of the B-chain, or the CTP peptide is a connecting peptide that covalently links the B-chain to the A-chain to produce a single-chain insulin or insulin analogue having the structure (B-chain)-(CTP peptide)-(A-chain); and a pharmaceutically acceptable carrier or salt.