Patent ID: 9664685
Date: 2017-05-30
CPC Classifications: G01N

Claim:
1. A method for analyzing a protamine sample for the presence or amount of at least one nucleic acid, comprising the steps of: (a) providing a protamine sample containing at least one nucleic acid, (b) subjecting the sample of protamine to disruption with trypsin to provide a first mixture, wherein the first mixture comprises trypsin, protamine, and at least one nucleic acid, (c) heating the first mixture under conditions sufficient to inactivate the trypsin to provide a second mixture, wherein the second mixture comprises inactivated trypsin, protamine, and at least one nucleic acid, (d) subjecting the second mixture to a digestion step to provide a third mixture, and (e) determining the presence or amount of at least one compound derived from the nucleic acid present in the third mixture.