Patent ID: 9598692
Date: 2017-03-21
CPC Classifications: C12N,C40B

Claim:
1. A method for multivalent phage display comprising: a) providing a non-suppressor host strain comprising a phagemid encoding an open reading frame comprising a pIX nucleic acid sequence operably linked to a nucleic acid sequence encoding a protein of interest, wherein expression of said open reading frame results in production of a protein of interest-pIX fusion protein; b) providing a helper phage comprising a nucleotide sequence encoding an open reading frame comprising a pIX nucleic acid sequence, wherein said pIX nucleic acid sequence comprises at least one non-wildtype suppressible stop codon located between the first two N-terminal amino acids and the last eight C-terminal amino acids in the pIX open reading frame; and c) infecting said non-suppressor host strain with said helper phage under conditions such that said non-suppressor host cell produces phages wherein all copies of wild-type pIX are replaced by said protein of interest-pIX fusion protein.