Patent ID: 9567624
Date: 2017-02-14
CPC Classifications: C12N,C12Q,C12Y,Y10T

Claim:
1. A method for determining the amount or activity of a first luciferase and a second luciferase in a sample, the method comprising (a) contacting the sample with a reactive substrate for the first luciferase and a reducing agent that time dependently inactivates the first luciferase but does not inactivate the second luciferase; (b) measuring the luminescent signal before the first luciferase is inactivated; (c) contacting the sample with a reactive substrate for the second luciferase; (d) measuring the luminescent signal after the first luciferase is inactivated; and (e) using the data collected in (b) and (d), calculating the amount or activity of each luciferase in the sample relative to the other luciferase or relative to the same luciferase in another sample.