Patent ID: 9730964
Date: 2017-08-15
CPC Classifications: A61K,A61P

Claim:
1. A method of treating acne in a subject in need thereof, comprising topically administering to the subject a pharmaceutical composition comprising an effective amount of adipose tissue-derived secretions, in combination with a pharmaceutically-acceptable carrier or diluent, wherein the adipose tissue-derived secretions are prepared by: (i) exposing a sample of bovine adipose tissue to a proteolytic enzyme solution to generate a cell suspension; (ii) centrifuging the suspension of cells to form a cell pellet, a free lipid layer above a floating cell layer which comprises adipocytes and an intermediate layer between the cell pellet and the floating cell layer, said intermediate layer being depleted of cells relative to the cell pellet and the floating cell layer; and (iii) removing the free lipid layer and the intermediate layer; (iv) optionally removing adipocytes from the centrifuged material by removing part or all of the floating cell layer which comprises adipocytes; (v) mixing the cell pellet and, if present, the floating cell layer comprising adipocytes, to form an adipose tissue derived cell suspension which may or may not include adipocytes; (vi) culturing the cell suspension under appropriate conditions; (vii) collecting a supernatant of the cell culture to obtain the adipose tissue-derived secretions.