Patent ID: 9657330
Date: 2017-05-23
CPC Classifications: C12Q,G01N

Claim:
1. A composition comprising a residual supernatant from a human stool homogenate comprising human DNA and DNA from gut-resident bacteria, said residual supernatant produced by a method comprising: i) preparing a stool supernatant fluid from said human stool homogenate, wherein said stool supernatant fluid is produced by homogenizing a human stool sample with a buffer to produce said human stool homogenate, and treating said human stool homogenate to partition residual solids from a fluid fraction, wherein said fluid fraction is said stool supernatant fluid; ii) treating the stool supernatant fluid with insoluble polyvinylpolypyrrolidone to adsorb and remove assay inhibitors, and removing said insoluble polyvinylpolypyrrolidone to produce treated stool supernatant fluid, iii) adding guanidine thiocyanate to said treated stool supernatant fluid to a concentration of 2-3 M guanidine thiocyanate; iv) exposing treated stool supernatant fluid comprising guanidine thiocyanate to a plurality of different target-specific capture reagents, under conditions wherein each of said plurality of different target-specific capture reagents forms a complex with a different target human DNA, wherein each target-specific capture reagent comprises an oligonucleotide covalently attached to a particle, said oligonucleotide complementary to at least a portion of a target human DNA, and wherein said exposing comprises incubating said treated stool supernatant fluid comprising guanidine thiocyanate with said target-specific capture reagents to produce target-specific capture reagent/target human DNA complexes, and v) removing target-specific capture reagent/target human DNA complexes from said treated stool supernatant fluid to produce said residual supernatant,