Patent ID: 9615550
Date: 2017-04-11
CPC Classifications: A01K,C07K

Claim:
1. A mouse whose genome comprises at an endogenous H-2K locus a nucleotide sequence encoding a chimeric human/mouse HLA-A/H-2K polypeptide, wherein the nucleotide sequence is in operable linkage with an endogenous mouse regulatory element and is operably linked to a MHC class I leader encoding sequence, wherein the nucleotide sequence comprises from 5′ to 3′ (i) a first nucleic acid sequence that encodes the α1, α2 and α3 domains of the human HLA-A polypeptide, which first nucleic acid sequence replaces a sequence encoding the α1, α2, and α3 domains of the mouse H-2K polypeptide at the mouse H-2K locus and (ii) a second nucleic acid sequence that encodes the transmembrane and cytoplasmic domains of the mouse H-2K polypeptide, wherein the mouse expresses, on the surface of nucleated cells, from the endogenous H-2K locus the chimeric human/mouse HLA-A/H-2K polypeptide that comprises the α1, α2 and α3 domains of the human HLA-A polypeptide and the transmembrane and cytoplasmic domains of the mouse H-2K polypeptide.