Patent ID: 9598480
Date: 2017-03-21
CPC Classifications: C07K,C12N

Claim:
1. A method of expressing a peptide in a mammalian cell comprising: providing a nucleic acid construct including at least a nucleic acid encoding at least a NEL-like protein (NELL) peptide in frame with a nucleic acid encoding a heterologous, non-insect secretory signal peptide; transfecting a mammalian cell with the nucleic acid construct; culturing the mammalian cell under conditions that permit expression of the NELL peptide; collecting NELL peptide secreted from the cell; and substantially purifying the NELL peptide using a chromatography process that uses membrane chromatography or affinity chromatography; wherein the nucleic acid encodes NELL1 or NELL2, wherein NELL1 is selected from the group consisting of: SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5, and SEQ ID NO:6, wherein NELL2 is selected from the group consisting of: SEQ ID NO:7, SEQ ID NO:8, SEQ ID NO:9, SEQ ID NO:10, SEQ ID NO:11, SEQ ID NO:12, SEQ ID NO:13, and SEQ ID NO:14.