Patent ID: 9699976
Date: 2017-07-11
CPC Classifications: A01H,A01N,C12N

Claim:
1. A method of regenerating Jatropha via organogenesis comprising the steps: (a) culturing a (b) culturing the shoot buds on a solid shoot bud proliferation and development medium to proliferate and develop the shoot buds, wherein the shoot bud proliferation and development comprises MS basal salts, B5 vitamins, IBA, TDZ, BA, zeatin (Zn), adenine sulfate (AdSO (c) culturing a shoot bud clump derived from the developed shoot buds on a solid shoot elongation medium to elongate shoots, wherein the shoot elongation medium comprises MS basal salts, B5 vitamins, BA, kinitin (Kn), gibberellic acid (GA (d) culturing elongated shoots separated from the shoot bud clump on a solid shoot maturation medium to mature the shoots, wherein the shoot maturation medium comprises MS basal salts, B5 vitamins, IBA, Kn, GA (e) dipping the mature shoots in a liquid growth regulator solution and transferring the dipped shoots to soil for growth of