Patent ID: 9617329
Date: 2017-04-11
CPC Classifications: C07K,C12N,G01N

Claim:
1. A method for producing an expression construct capable of expressing a plurality of polypeptides, the method comprising: providing the following unlinked nucleic acids: (i) a first nucleic acid encoding a first polypeptide; (ii) a second nucleic acid encoding a second polypeptide; and (iii) a third nucleic acid encoding a marker protein that facilitates detection and/or isolation of a cell expressing the marker protein; providing an expression vector, which does not comprise a nucleic acid encoding the marker protein encoded by the nucleic acid at (iii); and linking said unlinked nucleic acids and said expression vector in a single cloning cycle, wherein said linking comprises ligase dependent cloning or ligase independent cloning, and wherein said first polypeptide and said second polypeptide associate to form a multimeric protein.