Patent ID: 9554525
Date: 2017-01-31
CPC Classifications: A01H,A61P,C12N,C12Q

Claim:
1. A method of predicting the phenotype of a soybean plant for glycinin and β-conglycinin content, said method comprising assaying the soybean plant for the presence of a non-transgenic mutant Gy1 allele comprising a deletion of the Gy1 allele found in line B2G2, a representative sample of seeds thereof is deposited under ATCC Accession No. PTA-6893; assaying the soybean plant for the presence of a non-transgenic mutant Gy2 allele comprising a deletion of the Gy2 allele found in line B2G2; or assaying the soybean plant for the presence of a non-transgenic mutant Gy3 allele selected from the group consisting of an insertion at position 848-851, a single nucleotide polymorphism (SNP) at position 1083, a SNP at position 1120, and a SNP at position or 1866; wherein the position is the position in said allele corresponding to SEQ ID NO:164; wherein the soybean plant comprising the non-transgenic mutant Gy1, Gy2, Gy3, or Gy4 allele comprises decreased glycinin and increased β-conglycinin content relative to a soybean plant that does not comprise the non-transgenic mutant Gy1, Gy2, Gy3, or Gy4 allele.