Patent ID: 9593305
Date: 2017-03-14
CPC Classifications: C12N

Claim:
1. A method of increasing the yield of cells expressing markers characteristic of the definitive endoderm lineage by differentiating human pluripotent stem cells into cells expressing markers characteristic of the definitive endoderm lineage, the method comprising treating the human pluripotent stem cells with a medium lacking activin A, and containing GDF-8 and 14-Prop-2-en-1-yl-3,5,7,14,17,23,27-heptaazatetracyclo[19.3.1.1˜2,6˜.1˜8,12˜]heptacosa-1(25),2(27),3,5,8(26),9,11,21,23-nonaen-16-one, for a period of time sufficient for the human pluripotent stem cells to differentiate into cells expressing markers characteristic of the definitive endoderm lineage, wherein the method increases the percentage of cells expressing CXCR4.