Patent ID: 9593306
Date: 2017-03-14
CPC Classifications: C12N

Claim:
1. A method to differentiate pluripotent stem cells into cells expressing markers characteristic of the definitive endoderm lineage, comprising treating the pluripotent stem cells with a medium lacking activin A, and containing GDF-8 and a cyclic analine-pyridinotriazine comprising 5-Chloro-1,8,10,12,16,22,26,32-octaazapentacyclo[24.2.2.1˜3,7˜.1˜9,13˜.1˜14,18˜]tritriaconta-3(33),4,6,9(32),10,12,14(31),15,17-nonaen-23-one, for a period of time sufficient for the pluripotent stem cells to differentiate into cells expressing markers characteristic of the definitive endoderm lineage.