Patent ID: 9532554
Date: 2017-01-03
CPC Classifications: A01K,C12N,G01N

Claim:
1. A method for producing an immunodeficient non-human animal having a humanized liver expressing a human-derived drug-metabolizing enzyme, comprising: transplanting human hepatic stem cells, human hepatic progenitor cells, and/or human immature hepatocytes to a liver-damaged immunodeficient non-human animal via spleen or portal vein to induce the differentiation of the cells into hepatocytes, wherein the human hepatic stem cells, human hepatic progenitor cells, and/or human immature hepatocytes are CDCP1-positive/CD90-positive/CD66-negative cells, wherein the non-human animal is selected from the group consisting of a TRECK mouse and an uPA-NOG mouse, wherein the TRECK mouse has a high chimera rate of human hepatocytes of up to 99.9%, and wherein the uPA-NOG mouse has a high chimera rate of human hepatocytes of up to 93.3%, and wherein the TRECK mouse has a survival time exceeding 120 days.