Patent ID: 9725754
Date: 2017-08-08
CPC Classifications: C12Q

Claim:
1. A method for simultaneously isolating a viral target nucleic acid and a bacteria target nucleic acid from a plurality of biological samples comprising at least two different types of biological samples, the method comprising: (a) adding an identical lysis buffer to the plurality of biological samples comprising at least two different types of biological samples, wherein the at least two different types of biological samples comprise at least a first biological sample comprising a viral target nucleic acid from a first human body fluid and a second biological sample comprising a bacterial target nucleic acid from a second human body fluid, wherein the lysis buffer has a pH between 5.5 and 6.5 and comprises a chaotropic agent a buffer substance, an alcohol and a reducing agent; and combining a solid support material with the plurality of biological samples in a number of vessels corresponding to the number of biological samples, for a period of time and under conditions sufficient to permit the viral target nucleic acid and the bacteria target nucleic acid to be immobilized on the solid support material, wherein the period of time and conditions are identical for the plurality of biological samples; (b) isolating the solid support material of step (a) from other material that is present in the biological samples, thereby producing isolated solid support material; (c) washing the isolated solid support material of step (b) one or more times with a wash buffer by aspirating and dispensing suspensions comprising the wash buffer and the isolated solid support material one or more times using a pipette; and (d) separating the viral target nucleic acid and the bacteria target nucleic acid from the solid support material;