Patent ID: 9582640
Date: 2017-02-28
CPC Classifications: C12Q,G16B

Claim:
1. A method of obtaining a single molecule consensus sequence of a single template nucleic acid molecule, comprising: performing a single-molecule sequencing process on a single template nucleic acid molecule, wherein the single template nucleic acid molecule comprises: i) a first strand having a 3′ end and a 5′ end, ii) a second strand, complementary to the first strand, and having a 3′ end and a 5′ end, and iii) a linker that links the 3′ end of the first strand to the 5′ end of the second strand, wherein the linker comprises a linking oligonucleotide, thereby generating a sequence read from said single template nucleic acid molecule, the sequence read comprising a first sequence corresponding to the sequence of the first strand, and a second sequence corresponding to the sequence of the second strand; and comparing the first sequence and the second sequence from said sequence read, thereby obtaining a single molecule consensus sequence of the single template nucleic acid molecule.