Patent ID: 9732381
Date: 2017-08-15
CPC Classifications: C12Q,G01N

Claim:
1. A method for sequencing a heteropolymeric target nucleic acid sequence, comprising: (a) passing the heteropolymeric target nucleic acid sequence through a transmembrane protein pore so that the nucleotides in the heteropolymeric target nucleic acid sequence interact with two or more distinct sites of amino acids within the sequence of the transmembrane protein pore that are capable of discriminating between different nucleotides, such that the overall current passing through the pore is influenced by the interactions between each of the two or more sites and the nucleotides located at each of the sites; wherein: (1) the pore has been determined to comprise at least two of said distinct sites of amino acids, (2) at least one of the distinct site of amino acids is modified by amino acid substitution to alter its ability to discriminate between different nucleotides, and (3) the heteropolymeric target nucleic acid sequence is not cleaved or digested to form individual nucleotides before the heteropolymeric target nucleic acid sequence is sequenced; and (b) measuring the overall current passing through the pore, thereby determining the sequence of the heteropolymeric target nucleic acid sequence.