Patent ID: 9683256
Date: 2017-06-20
CPC Classifications: C12N,C12Q

Claim:
1. A stock solution comprising components with concentrations as follows: a chaotrope present in an amount from 0.5 M to 6M; a detergent present in an amount from 0.1% to 1% (wt./vol.); a reducing agent in an amount from 0.5 mM to 0.3 M; a chelator present in an amount from 0.01 mM to 50 mM; a surfactant present in an amount from 0.0001% to 0.3% (wt./vol.); a short-chain alkanol present in an amount from 1 to 25% (vol./vol.); a buffer present in an amount from 1 mM to 1 M; an acid or base present in an amount that provides a pH of from 5 to 7 to the stock solution; and nuclease-free water, wherein the stock solution, when combined with a biological sample suspected of containing a pathogen, denatures proteins, inactivates nucleases, and kills pathogens all without degrading nucleic acids of the sample.