Patent ID: 9605323
Date: 2017-03-28
CPC Classifications: A23K,A61P,C12N,C12P,C12R

Claim:
1. A method for producing carotenoids comprising: 1) culturing a bacterial strain in a culture medium containing biotin at a concentration of 0.02 mg/L to 50 mg/L by addition of biotin to the culture medium, wherein the bacterial strain is a member of the genus 2) increasing the concentration of dissolved oxygen in the culture medium in a stepwise or continuous manner during the intermediate phase of culture, wherein the concentration of dissolved oxygen in the culture medium at the beginning of the intermediate phase of culture before increasing the concentration is 1.5 ppm to 2.5 ppm, and the concentration of dissolved oxygen in the culture medium at the end of the intermediate phase of culture after increasing the concentration is 3 ppm to 6 ppm, thereby producing a carotenoid-containing culture broth in which the ratio of the concentration of canthaxanthin to the concentration of astaxanthin in the culture broth at the end of the culture period is 8% to 15% and the ratio of the concentration of adonixanthin to the concentration of astaxanthin in the culture broth at the end of the culture period is 25% or less; and 3) collecting the carotenoids from the carotenoid-containing culture broth.