Patent ID: 9664695
Date: 2017-05-30
CPC Classifications: G01N

Claim:
1. A method for (a) diagnosing or monitoring kidney function in a subject or (b) diagnosing kidney dysfunction in a subject or (c) predicting or monitoring the risk of an adverse event in a subject with kidney disease wherein said adverse event is selected from the group comprising worsening of kidney dysfunction including kidney failure, loss of kidney function and end-stage kidney disease or death due to kidney dysfunction including kidney failure, loss of kidney function and end-stage kidney disease or (d) predicting or monitoring the success of a therapy or intervention in a subject with kidney disease comprising: determining the level of Pro-Enkephalin or fragments thereof in a bodily fluid obtained from said subject; and (a) correlating said level of Pro-Enkephalin or fragments thereof with kidney function in a subject, or (b) correlating said level of Pro-Enkephalin or fragments thereof with kidney dysfunction, wherein an elevated level above a certain threshold is predictive or diagnostic for kidney dysfunction in said subject, or (c) correlating said level of Pro-Enkephalin or fragments thereof with said risk of an adverse event in a subject with a kidney disease, wherein an elevated level above a certain threshold is predictive for an enhanced risk of said adverse events, or (d) correlating said level of Pro-Enkephalin or fragments thereof with success of a therapy or intervention in a subject with a kidney disease, wherein a level below a certain threshold is predictive for a success of therapy or intervention, wherein said Pro-Enkephalin or fragment is one or more of SEQ ID No. 1 or SEQ ID No. 6, wherein the level of Pro-Enkephalin or fragments thereof is determined by using an assay comprising two binders that bind to two different regions within the region of Pro-Enkephalin that is amino acid 133-140 (LKELLETG, SEQ ID NO. 13) and amino acid 152-159 (SDNEEEVS, SEQ ID No. 14), wherein each of said regions comprises at least 4 amino acids.