Patent ID: 9575077
Date: 2017-02-21
CPC Classifications: B01L,C07K,G01N

Claim:
1. A method for performing a thyroid stimulating hormone (TSH) immunoassay, the method comprising the steps of: (a) inserting a fluid sample into a device comprising an immunosensor in a conduit, said immunosensor having a capture antibody specific for TSH immobilized thereon; (b) dissolving a signal antibody into said fluid sample, wherein the capture antibody and the signal antibody each have a dissociation constant (Kd) for TSH of between 0.08 and 0.1 nM; (c) forming a sandwich complex on said immunosensor, said complex comprising said capture antibody, TSH from the fluid sample, and said dissolved signal antibody; and (d) detecting a signal associated with said complexed signal antibody as indicative of a level of TSH in the fluid sample, wherein the capture antibody is mouse monoclonal anti-TSH antibody 5409 and the signal antibody is mouse monoclonal anti-TSH antibody M94206.