Patent ID: 9605318
Date: 2017-03-28
CPC Classifications: A61P,C12Q,G01N

Claim:
1. A method for predicting the response of a human patient diagnosed with breast cancer to chemotherapy and of treating the patient comprising: extracting RNA from a fresh, frozen, or fixed, paraffin-embedded tissue (FPET) sample comprising cancer cells obtained from said patient; reverse transcribing an RNA transcript of GBP1 to produce cDNA of GBP1; amplifying the cDNA of GBP1 using a PCR-based method to produce an amplicon of the RNA transcript of GBP1; quantitatively assaying a level of the amplicon of the RNA transcript of GBP1; normalizing said level against a level of an amplicon of at least one reference RNA transcript in the sample to provide a normalized GBP1 amplicon level; comparing the normalized GBP1 amplicon level to a normalized GBP1 amplicon level in reference samples comprising breast cancer cells; predicting the response of said patient to chemotherapy, wherein the patient is predicted to have an increased likelihood of response to chemotherapy if the patient has an increased normalized GBP1 amplicon level compared to the GBP1 amplicon level in the reference samples; and administering chemotherapy to the patient with an increased normalized GBP1 amplicon level compared to the GBP1 amplicon level in the reference samples.