Patent ID: 9701993
Date: 2017-07-11
CPC Classifications: C12N,C12P

Claim:
1. A method for synthesizing peptides using dual sense codons and artificial division of codon boxes, comprising: i) combining at least the following in an in vitro translation system: ii) synthesizing at least one peptide comprising a (b) artificial amino acid, a (c) natural amino and a (d) artificial amino acid using the mRNAs and tRNA(s) in the in vitro translation system, wherein a codon for a (b) initiator tRNA of the (b) artificial amino acid is the same as a codon for a (c) elongator tRNA of the (c) natural amino acid or a codon for a (d) elongator tRNA of the (d) artificial amino acid, and wherein the (b) artificial amino acid and the (d) artificial amino acid in the at least one peptide are different if the codon for the (b) initiator tRNA is the same as the codon for the (d) elongator tRNA.