Patent ID: 9675972
Date: 2017-06-13
CPC Classifications: B01F,B01L,B03C,Y10T

Claim:
1. A method of concentrating beads in a droplet, the method comprising: (a) providing a droplet actuator comprising: (b) establishing a droplet in the liquid path extending from the reservoir into the interior volume; (c) providing a first concentration of magnetically responsive beads in the portion of the droplet which is in the reservoir; (d) magnetically attracting the magnetically responsive beads through the liquid path into the portion of the droplet which is in the interior volume; and (e) forming a droplet comprising a second concentration of magnetically responsive beads in the interior volume, wherein the second concentration of magnetically responsive beads in the droplet formed in the interior volume is greater than the first concentration of magnetically responsive beads provided in the portion of the droplet which is in the reservoir; and (f) conducting one or more droplet operations on the droplet in the interior volume via electrowetting.