Patent ID: 9587273
Date: 2017-03-07
CPC Classifications: C12N,C12Q

Claim:
1. A method of selecting a representational sample of nucleic acid sequences from a mixture of nucleic acids, comprising: (a) providing a mixture of nucleic acids, wherein the mixture of nucleic acids comprises cDNA; (b) providing a population of solid support-attached capture probes, said population comprising nucleic acids complementary to nucleic acids comprising a predetermined portion of the nucleic acid sequences collectively present in said mixture of nucleic acids; (c) contacting said mixture of nucleic acids with said population of solid support-attached capture probes under conditions sufficient for hybridization of said nucleic acids with said capture probes, wherein the capture probes are randomly located on the solid support; (d) removing unhybridized nucleic acids from the solid support to separate desired sequences from undesired sequences within the mixture; thereby selecting a representational sample of nucleic acids, wherein the proportion of each sequence in said representational sample relative to all other sequences in said representational sample is substantially the same as the proportions of the sequences in said mixture; (e) amplifying the nucleic acids in the representational sample on the solid support to generate an amplified representational sample; said amplifying maintaining separation of sequences by attachment to identifiable locations on the solid support; and (f) performing a sequence analysis of the amplified representational sample.