Patent ID: 9732363
Date: 2017-08-15
CPC Classifications: C12N,C12P,Y02E

Claim:
1. A growth method for a microbe in a saccharified solution containing a saccharification product, comprising steps of: obtaining a mixed saccharified solution by diluting the saccharified solution containing a growth inhibitor which inhibits the growth of the microbe with a sugar solution having a smaller growth inhibitor concentration than that of the saccharified solution such that the growth inhibitor concentration is decreased; and allowing the microbe to grow by adding the microbe to the mixed saccharified solution, wherein obtaining the mixed saccharified solution comprises: a first step of culturing the microbe using a plurality of culture media in which, with respect to each growth inhibitor of a plurality of growth inhibitors contained in the saccharified solution, a sugar solution for testing is supplemented with the growth inhibitor at respective different growth inhibitor concentrations, and measuring a number of grown microbes for each of the growth inhibitor concentration contained in each culture medium with respect to each growth inhibitor; a second step of culturing the microbe using the sugar solution for testing unsupplemented with each growth inhibitor as a culture medium, and measuring a number of grown microbes in the unsupplemented sugar solution for testing to prepare a reference number of grown microbes; a third step of calculating a rate of microbial growth at each growth inhibitor concentration with respect to each growth inhibitor by dividing the number of grown microbes for each of the growth inhibitor concentration with respect to each growth inhibitor by the reference number of grown microbes; a fourth step of preparing a first growth inhibition curve from a relationship between the growth inhibitor concentration of each culture medium and the rate of microbial growth with respect to each growth inhibitor; a fifth step of measuring each growth inhibitor concentration of the saccharified solution; a sixth step of determining the rate of microbial growth corresponding to the growth inhibitor concentration of the saccharified solution with respect to each growth inhibitor from the first growth inhibition curve with respect to each growth inhibitor; a seventh step of, as to each of the mixed saccharified solutions in which the saccharified solution in an undiluted state is diluted with the sugar solution in respective different amounts, determining the rate of microbial growth corresponding to a saccharified solution ratio of each mixed saccharified solution with respect to each growth inhibitor from the first growth inhibition curve of each growth inhibitor, when the saccharified solution ratio of the undiluted saccharified solution is defined as 1, and then calculating the rate of microbial growth corresponding to the saccharified solution ratio of each mixed saccharified solution by multiplying the respective rates of microbial growth of each of the growth inhibitors in the mixed saccharified solutions having the same saccharified solution ratio; an eighth step of preparing a second growth inhibition curve from a relationship between the saccharified solution ratio of each mixed saccharified solution and the rate of microbial growth; a ninth step of determining a saccharified solution ratio corresponding to a desired rate of microbial growth from the second growth inhibition curve; and a tenth step of obtaining the mixed saccharified solution by diluting the saccharified solution with the sugar solution such that the saccharified solution ratio obtained in the ninth step is achieved.