Patent ID: 9580482
Date: 2017-02-28
CPC Classifications: A61K,A61P,C07K,Y02A

Claim:
1. A Plasmodium falciparum Thrombospondin-Related Anonymous Protein (TRAP) antigen that is at least 80% identical to amino acids 25-574 of SEQ ID NO:5, wherein the antigen sequence comprises one or more of the following mutations or deletions: (a) Mutation at Cysteine 55 to a non-cysteine amino acid; (b) Mutation of N-linked glycosylation sites; (c) Mutation of Ala-216/Asn-222 or Lys-224/Gln-78 to cysteine to create a TRAP that is stabilized in the open conformation; (d) Mutation of Asn-213/Ala-233, Ala-216/Phe-230, or Met-231/Gln-78 to cysteine to create a TRAP that is stabilized in the closed conformation; (e) Deletion of N-terminal and/or C-terminal residues to create a TRAP fragment that is stabilized in the closed conformation comprising V47-V238; (f) Deletion of N-terminal and/or C-terminal residues to create a TRAP fragment that is stabilized in the open conformation comprising V47-M231, and wherein the antigen can elicit an immune response in a mammalian subject.