Patent ID: 9546400
Date: 2017-01-17
CPC Classifications: B82Y,C12Q,G01N

Claim:
1. A method for sequencing a nucleic acid template comprising: a) providing a substrate comprising a nanopore in contact with a solution, the solution comprising a template nucleic acid above the nanopore; b) providing a voltage across the nanopore; c) measuring a property which has a value that varies for N monomeric units of the template nucleic acid in the pore, wherein the measuring is performed as a function of time, while the template nucleic acid is translocating through the nanopore, wherein N is three or greater; and d) determining the sequence of the template nucleic acid using the measured property from step (c) by performing a process including comparing the measured property from step (c) to calibration information produced by measuring such property for 4 to the N sequence combinations.