Patent ID: 9678076
Date: 2017-06-13
CPC Classifications: G01N

Claim:
1. A method of treating a subject comprising: partitioning a first sample arising from a subject in a first aqueous two-phase partitioning system, wherein the first sample is blood; measuring a first distribution of human serum albumin (HSA) within the phases of the first partitioning system and determining a first partition coefficient of the first partitioning system; partitioning a second sample in a second aqueous two-phase partitioning system, wherein the second aqueous two-phase partitioning system is substantially chemically identical to the first aqueous two-phase partitioning system, wherein the second sample is a control; measuring a second distribution of HSA within the phases of the second partitioning system, and determining the second partition coefficient of the second partition coefficient; identifying the presence of a ligand attached to HSA in the first sample based on a difference between the first distribution of HSA and the second distribution of HSA, as determined by the difference in the partition coefficients; and treating the subject with an anti-cancer drug based on the presence of the ligand attached to the HSA.