Patent ID: 9714915
Date: 2017-07-25
CPC Classifications: C12N,C12Q,C12Y,G01N

Claim:
1. A system for nucleic acid sequencing, comprising: (a) a field effect transistor (FET); and (b) a modified Bst DNA polymerase comprising three or more amino acid substitutions selected from the group consisting of H46R, H273R, H281R, E446Q, H473R, H528R, H572R and Y477F, the numbering of amino acid residues being in accordance with that of SEQ ID NO. 1, wherein the modified polymerase has reduced buffering capacity compared to wild type Bst DNA polymerase, is configured to catalyze nucleotide polymerization, and comprises an amino acid sequence having at least 90% identity to the amino acid sequence of SEQ ID NO. 1; and wherein the FET comprises an ion sensitive FET (ISFET) configured to detect the release of hydrogen ions as a chemical byproduct of nucleotide incorporation catalyzed by the modified polymerase.