Patent ID: 9683210
Date: 2017-06-20
CPC Classifications: A61K,A61P,C12M

Claim:
1. A method for separating cells comprising: a) adding an amount of tissue comprising fat cells to an inner bow of a cell separation apparatus, the cell separation apparatus comprising: (i) a medium reservoir; (ii) a cell processing device comprising (A) at least one inlet and (B) at least one outlet, (C)a first lobe and (D) a second lobe; (iii) at least one pump; and (iv) at least one valve adapted to divert or prevent fluid flow, wherein the at least one pump and at least one valve are in fluid communication with one another; and wherein the inner bowl is in fluid communication with an outer bowl; b) heating the tissue to a temperature of about 37°C; c) adding an effective amount of collagenase to the inner bowl; d) digesting the tissue by oscillating the inner bowl for an effective amount of time; e) removing fibrous tissue from the digested tissue; f) spinning the inner bowl at about 3100 RPM for about 5 minutes; g) displacing the fat cells to the center of the inner bowl by adding fluid to the inner bowl; h) directing the fat cells through at least one aperture in the inner bowl into the outer bowl by centrifugal action; i) rinsing the inner bowl with fluid; j) directing at least one cell pellet from at least one lobe of the inner bowl to the bottom or the inner bowl; k) optionally, adding fluid to the inner bowl and spinning the inner bowl at about 3100 RPM for about 5 minutes; and l) collecting the at least one cell pellet.