Patent ID: 9745619
Date: 2017-08-29
CPC Classifications: B01J,C07B,C07H,C12N,C12Q,C40B,G01N

Claim:
1. A process for detecting or quantifying more than one nucleic acid of interest in a library comprising the steps of: a) providing: b) contacting said library of nucleic acid analytes with said first set of primers to form a first set of bound primers; c) extending said first set of bound primers by means of template sequences provided by said nucleic acid analytes to form first copies of said analytes; d) contacting said extended first copies with said second set of primers to form a second set of bound primers; e) extending said second set of bound primers by means of template sequences provided by said extended first copies to form second copies of said nucleic acid analytes; f) contacting said second copies with said third set of primers to form more than one third bound entity to form a third set of bound primers; g) extending said third set of bound primers by means of template sequences provided by said extended second set of primers to form a hybrid comprising a second copy, a third copy and at least one production center; h) synthesizing from a production center in said third set of primers in said complexes one or more nucleic acid copies under isothermal or isostatic conditions; i) hybridizing said nucleic acid copies synthesized in step h) to said array of nucleic acids provided in step a) (i); and j) detecting or quantifying any of said hybridized copies obtained in step i).