Patent ID: 9631227
Date: 2017-04-25
CPC Classifications: C12N,C12Q

Claim:
1. A method for preparing a nucleic acid library that reduces or inhibits acceptor:donor probe dimer formation, said method comprising: incubating target nucleic acid fragments in a reaction mixture comprising a ligase, a modified donor probe and a modified acceptor probe to prepare a nucleic acid library, wherein said nucleic acid library contains target nucleic acid fragments comprising said modified donor probe ligated to the 3′-terminus and said acceptor probe ligated to the 5′terminus, wherein dimerization of said modified donor probe to said modified acceptor probe is reduced or inhibited and wherein said modified donor probe has the formula 5′-phosphate-X (n1) -Y (n2) Z (n3) -3′ or 5′-adenylate-X (n1) -Y (n2) -Z (n3) -3′ and said modified acceptor probe has the formula 5′-D (n4) -E (n5) -F (n6) -hydroxy-3′, wherein (n2), (n3), (n4), and (n5) are each independently any positive integer, wherein (n1) and (n6) are each independently 1, 2, 3 or 4, wherein X, Y, Z, D, E, and F are nucleotide positions, wherein at least one of X (n1) , at least one of Z (n3) and at least one of F (n6) nucleotide positions are modified.