Patent ID: 9747412
Date: 2017-08-29
CPC Classifications: C12Q,G01N,G16B

Claim:
1. A method for identifying differential activation between samples of a protein transferase having a non-protein donor substrate and a protein acceptor substrate; said method comprising: (i) exposing a first sample to x different concentrations of the non-protein donor substrate of said protein transferase, wherein x is 2 or greater than 2; (ii) quantifying modification of a polypeptide in said first sample at each of the x different concentrations of the non-protein donor substrate; (iii) determining the affinity of said protein transferase for said non-protein donor substrate; (iv) repeating steps (i) to (iii) for a second or subsequent sample; and (v) comparing the affinity of said protein transferase for said non-protein donor substrate between said samples; wherein a difference in affinity of said protein transferase for said non-protein donor substrate between samples is indicative of differential activation of said protein transferase between samples.