Patent ID: 9556431
Date: 2017-01-31
CPC Classifications: A61K,C07H,C12N

Claim:
1. A bifunctional RNA molecule that comprises a single contiguous sequence or multiple distinct sequences that, individually or collectively, encode two or more RNA molecules, comprising: a first RNA molecule comprises a first double-stranded sequence, which comprises a first guide strand sequence that is complementary to a portion of an mRNA transcript encoded by the target gene; and a second RNA molecule comprises a second double-stranded sequence, which comprises a second guide strand sequence that is partially complementary to a portion of the mRNA transcript encoded by the target gene, wherein the second guide strand sequence comprises one or more bases that are mismatched with a nucleic acid sequence of the mRNA transcript encoded by the target gene, wherein the bifunctional RNA molecule activates cleavage-dependent and cleavage-independent RNA-induced silencing complex for reducing the expression level of the target gene.