Patent ID: 9599616
Date: 2017-03-21
CPC Classifications: A61K,B01L,C12M,C12N,G01N

Claim:
1. Method for the in vitro selection of at least one therapeutic highly migrative cell subpopulation from an original cell population of eukaryotic cells, wherein the therapeutic cell subpopulation can be used for the treatment of diseases of tissues or organs, and wherein the method has the following consecutive steps: a) adding an original cell population to a first starting reservoir, which is connected via at least one selection means having at least one opening to at least one second selection reservoir, wherein the at least one opening of the at least one selection means has a diameter of 3 μm to 500 μm; b) adding at least one cell culture medium to the at least one selection reservoir to which the therapeutic cell subpopulation migrates; c) culturing the original cell population for a period of 15 minutes to 48 hours, within which period a cell subpopulation migrates through the at least one opening of the selection means towards the at least one selection reservoir; d) selecting and obtaining a cell, which is selected from cells that migrated as the first to the one hundred thousandth cell through the at least one opening of the selection means into the at least one selection reservoir, in order to obtain a cell subpopulation with an elevated migration potential or tropism to a certain cell type compared to the original cell population; and e) selecting a highly migrative cell subpopulation from the cell subpopulation obtained in step d) using at least one agent that specifically binds to a cell marker that is a cell surface marker or an internal cell marker, thereby isolating the highly migrative therapeutic subpopulation.