Patent ID: 9720002
Date: 2017-08-01
CPC Classifications: G01N

Claim:
1. A method for evaluating an action of one or more compounds on a molecular flux rate through a muscle protein metabolic pathway in a living system, said method comprising: a) administering a first isotope-labeled substrate to a first living system, not exposed to said one or more compounds, for a period of time sufficient for said first isotope-labeled substrate to enter into and label at least one protein derived from muscle to produce at least one first isotope-labeled protein derived from muscle within said muscle protein metabolic pathway in said first living system; b) obtaining one or more samples from said first living system, wherein said one or more samples comprise said at least one first isotope-labeled protein derived from muscle; c) measuring an isotopic content, rate of incorporation, and/or pattern or rate of change in isotopic content and/or pattern of isotope labeling of said at least one first isotope-labeled protein derived from muscle; d) calculating a molecular flux rate through said muscle protein metabolic pathway based on the isotopic content, rate of incorporation, and/or pattern or rate of change of isotopic content and/or pattern of isotopic labeling in said at least one first isotope-labeled protein derived from muscle; e) exposing a second living system to said one or more compounds; f) administering a second isotope-labeled substrate to said second living system for a period of time sufficient for said second isotope-labeled substrate to enter into and label at least one protein derived from muscle to produce at least one second isotope-labeled protein derived from muscle; g) obtaining one or more samples from said second living system, wherein said one or more samples comprise said at least one second isotope-labeled protein derived from muscle; h) measuring an isotopic content, rate of incorporation, and/or pattern or rate of change in isotopic content and/or pattern of isotope labeling of said at least one second isotope-labeled protein derived from muscle; i) calculating a molecular flux rate through said muscle protein metabolic pathway in said second living system based on the isotopic content, rate of incorporation, and/or pattern or rate of change in isotopic content and/or pattern of isotope labeling of said at least one second isotope-labeled protein derived from muscle; and j) comparing said molecular flux rate through said muscle protein metabolic pathway in said first living system to said molecular flux rate through said muscle protein metabolic pathway in said second living system to evaluate the action of said one or more compounds on said molecular flux rate through the muscle protein metabolic pathway in said second living system.