Patent ID: 9649366
Date: 2017-05-16
CPC Classifications: A61K,A61P,C07K

Claim:
1. A method of purifying aplipooprotein A-I (APOA1) from plasma fraction IV comprising: suspending plasma fraction IV in a buffer to obtain a resulting supernatant, wherein the buffer has a pH level in the range of 3.0 to 10.0, and wherein the resulting supernatant contains APOA1; collecting the resulting supernatant; adding a sodium chloride solution to the resulting supernatant and obtaining an APOA1 precipitate solution; centrifuging the APOA1 precipitate solution and collecting a resulting APOA1 paste; suspending the resulting APOA1 paste and obtaining a resulting APOA1 suspension; filtering the resulting APOA1 suspension and obtaining a resulting filtered APOA1 suspension; and subjecting the resulting filtered APOA1 suspension to a chromatography process.