Patent ID: 9655839
Date: 2017-05-23
CPC Classifications: A61K,A61P,A61Q,C12Q,G01N

Claim:
1. A method for making a mixture of arginolytic bacterial strains for oral use, the method comprising: (a) obtaining a mixture of bacterial strains isolated from oral samples; (b) isolating and identifying arginolytic bacterial strains capable of producing ammonia via the arginine deiminase system (ADS); (c) conducting one or more separate assays to identify arginolytic bacterial strains capable of expressing ADS activity in at least one of the following assay conditions: in the absence of environmental arginine, in the presence of glucose, in a non-acidic pH, in aerobic conditions, and in the presence of at least one bacterial strain associated with dental caries; (d) selecting at least two different isolated arginolytic bacterial strains identified in step (c) to prepare a mixture of arginolytic bacteria; and (e) preparing a mixture of the at least two isolated arginolytic bacterial strains, wherein the mixture expresses ADS activity in at least two of the conditions, and wherein the mixture does not include any strains of