Patent ID: 9745568
Date: 2017-08-29
CPC Classifications: C12M,C12N

Claim:
1. A device for use in optoinjection transfection, the device comprising: a microdevice having a bottom and a center; a central vertical outlet formed within the microdevice at its center; more than two entrapping channels forming a plurality of entrapping channels positioned on the bottom of the microdevice and engaging the central vertical outlet, wherein each entrapping channel extends radially from the central vertical outlet and is aligned perpendicular to the central axis of the central vertical outlet; a piece of optical glass engaging the bottom of the microdevice and forming a seal with the plurality of entrapping channels; and a barrier engaging the optical glass, and forming a periphery around the microdevice, wherein the periphery is capable of retaining a solution of biological targets to be transfected; wherein the optical glass, plurality of entrapping channels and central vertical outlet are positioned so that a laser beam may pass through the optical glass to separately engage a target in each of the plurality of entrapping channels without passing through the central vertical outlet.