Patent ID: 9541551
Date: 2017-01-10
CPC Classifications: C07K,G01N

Claim:
1. A method of producing an anti-GAP43 antibody which is capable of distinguishing a non-phosphorylated threonine residue at position 89 (T89) from a phosphorylated threonine residue at position 89 (pT89) of mouse GAP43 set forth in SEQ ID NO: 13, and which is capable of specifically detecting a growth cone, the method comprising: injecting a polypeptide comprising at least a part of the mouse GAP43 set forth in SEQ ID NO: 13 to an animal; collecting antibodies from the animal; subjecting the antibodies to affinity purification; and studying the purified antibodies for a cross reactivity thereof and selecting an antibody which shows cross reactivity against a mouse GAP43 having a phosphorylated threonine residue at position 89 (pT89) and which does not show cross reactivity against a non-phosphorylated mouse GAP43 or a mouse GAP43 having a phosphorylated residue other than the threonine residue at position 89.