Patent ID: 9566583
Date: 2017-02-14
CPC Classifications: B01L

Claim:
1. A method for performing polymerase chain reactions (PCR), the method comprising: providing a sample block comprised of at least a first sample block sector and a second sample block sector wherein the second sample block sector is located adjacent the first sample block sector; cycling each of the first and second sample block sectors through a PCR thermal protocol wherein the protocol comprises two or more temperatures; measuring the temperature of the first sample block sector of the sample block; measuring the temperature of the second sample block sector of the sample block; calculating, with a control unit, a difference in temperature between the first sample block sector and the second sample block sector; and adjusting the temperature of the first sample block sector during the PCR thermal protocol based on the difference in temperature between the first sample block sector and the second sample block sector thereby increasing thermal uniformity across the adjacent sample block sectors.