Patent ID: 9745549
Date: 2017-08-29
CPC Classifications: C12N

Claim:
1. A cell culture substrate, which is used for culturing for induction into neural progenitor cells or neural cells, characterized in that, onto the surface, at least two proteins are immobilized or coated, wherein said at least two proteins comprise: a first protein which is selected from the group consisting of a fusion protein comprising an entire or partial region of N-cadherin, and a fusion protein comprising an entire or partial region of a protein homologous to N-cadherin; and a second protein which is selected from the group consisting of a protein belonging to cadherin family other than N-cadherin, a fusion protein comprising at least one extracellular (EC) domain selected from EC1 domain, EC2 domain, EC3 domain, EC4 domain and EC5 domain of a protein belonging to cadherin family other than N-cadherin, and a fusion protein comprising at least one extracellular (EC) domain selected from EC1 domain, EC2 domain, EC3 domain, EC4 domain and EC5 domain of a protein homologous to a protein belonging to cadherin family other than N-cadherin, wherein said protein belonging to cadherin family other than N-Cadherin is E-cadherin wherein the concentration of the first protein is 3-1000 μg/mL and the concentration of the second protein is 0.01-1000 μg/mL; ac4 wherein the substrate comprises polystyrene, polyethylene or polypropylene; and wherein said protein homologous to N-cadherin is a protein which comprises one or more of the EC1 domain, EC2 domain, EC3 domain, EC4 domain and EC5 domain, and which has hemophilic binding ability with N-cadherin.