Patent ID: 9745588
Date: 2017-08-29
CPC Classifications: C12N

Claim:
1. An isolated polynucleotide, comprising: at least a first and a second transcription termination signal, in any order, that are at most 1000 nucleotides apart, and at least one of the transcription termination signals comprises or encodes an RNA hairpin structure, said RNA hairpin structure comprises a stem of least 12 internal base pairs and wherein the RNA hairpin structure is defined by a sequence Y, X, Z, wherein Y is a nucleotide sequence of at least 16 nucleotides in length and with G/C content of at least 75%, X is the sequence U/T-U/T-CG, and Z is a nucleotide sequence with at least 80% complementarity to Y, the complementary nucleotides of Z being base paired with the nucleotides of Y, and X being a loop with no base pairing to Y or Z.