Patent ID: 9708619
Date: 2017-07-18
CPC Classifications: C12N,Y02A

Claim:
1. A method for silencing or attenuating expression of at least one target gene comprising introducing or expressing in a plant or a part thereof a chimeric ribonucleotide sequence comprising a modified ta-siRNA sequence, wherein said modified ta-siRNA sequence is modified from a natural ta-siRNA sequence which comprises at least one microRNA binding site and at least one phase region by: a) replacing the at least one phase region of said natural ta-siRNA sequence with a sequence which is substantially complementary to said at least one target gene and is heterologous to said natural ta-siRNA sequence; and b) replacing the at least one microRNA binding site of said natural ta-siRNA sequence with the sequence of a microRNA binding site which is substantially complementary to the sequence of a microRNA present in a plant; wherein said natural ta-siRNA sequence comprises a sequence selected from the group consisting of the sequence of SEQ ID NO: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, or 20 and a sequence having at least 98% identity to the sequence of SEQ ID NO: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, or 20.