Patent ID: 9670527
Date: 2017-06-06
CPC Classifications: C12Q

Claim:
1. A method for enriching a sample comprising nucleic acids for target nucleic acid analytes, comprising the steps of: synthesizing a set of probes for one or more analytes of interest, wherein the probes are complementary to plus or minus strands of a target nucleic acid analyte, wherein each probe has a 5′ universal priming site and a 3′ universal priming site; amplifying the set of probes using primers complementary to the 5′ universal priming site and primers complementary to the 3′ universal priming site; ligating the amplified probes to form double-stranded concatamers; isothermally amplifying the double-stranded concatamers to form single-stranded amplified concatamers; binding the single-stranded amplified concatamers to a solid support; contacting the solid support with the sample comprising nucleic acids under hybridization conditions so that complementary nucleic acids in the sample are captured on the solid support and non-complementary nucleic acids are removed; eluting captured nucleic acids from the solid support.