Patent ID: 9638700
Date: 2017-05-02
CPC Classifications: G01N

Claim:
1. A method for diagnosing a circulatory complication comprising cardiac arrhythmia and/or an ischemic complication comprising systolic blood pressure of less than 80 mmHg in a subject comprising: a) obtaining a serum or plasma sample from a subject suffering from an acute medical event, b) contacting, in vitro, a portion of a serum or plasma sample from said subject with an antibody immunoreactive for an NT-proANP peptide; c) contacting, in vitro, a portion of the same serum or plasma sample with two antibodies immunoreactive for different portions of a sFlt-1 peptide, wherein the first antibody has one of a biotin and a streptavidin linked thereto and wherein the second antibody has a Tris (2,2′-bipyridyl) ruthenium (H)-complex linked thereto; d) determining the amounts of NT-proANP peptide and sFlt-1 peptide in said sample based on the contacting steps of b) and c); e) comparing the amounts of the NT-proANP peptide and sFlt-1 peptide determined in step d) with reference amounts for NT-proANP peptide and sFlt-1 peptide, respectively; and f) diagnosing a circulatory complication comprising cardiac arrhythmia if the amount of NT-proANP-type peptide determined in step d) is greater than the reference amount of about 2500 pg/ml for NT-proANP peptide, and