Patent ID: 9663553
Date: 2017-05-30
CPC Classifications: B01D,C07K,C12N,Y02P

Claim:
1. An integrated process for the isolation and purification of plasma derived clotting Factor VIII, clotting Factor IX, albumin and immunoglobulin (IgG), without the use of ethanol precipitation, comprising: a) subjecting plasma to gel filtration chromatography to obtain three fractions (Fraction I, Fraction II and Fraction III); b) subjecting Fraction I and Fraction III to further chromatographic separations using anion and cation exchange resins; and c) subjecting to viral inactivation and recovering isolated and purified clotting Factor VIII from Fraction I, and clotting Factor IX, albumin and immunoglobulin (IgG) from Fraction III.