Patent ID: 9719989
Date: 2017-08-01
CPC Classifications: C12Q,G01N

Claim:
1. A method for quantitatively analyzing bacteria in a sample, comprising: introducing magnetic nanoparticles capable of capturing bacteria into the sample and allowing the magnetic nanoparticles to capture bacteria; separating the magnetic nanoparticles from the sample; passing the separated magnetic nanoparticles through a highly viscous medium in presence of a magnetic field to separate the magnetic nanoparticles into bacterium-complexed magnetic nanoparticles and free magnetic nanoparticles wherein the highly viscous medium is 10- to 1,000-fold higher in viscosity than water and the highly viscous medium is an aqueous solution containing polyvinylpyrrolidone in an amount of 10˜50 weight % or polyethylene glycol solution, and the size of the bacterium-complexed magnetic nanoparticles are bigger than the free magnetic nanoparticles; and quantitating the bacterium-complexed magnetic nanoparticles.