Patent ID: 9718768
Date: 2017-08-01
CPC Classifications: A61K,B01J,C07C,C07K,Y10T

Claim:
1. A method for binding a biomolecule, wherein the biomolecule comprises at least one primary amine group, to a support, the method comprising the step of: contacting a solution of the biomolecule with a mobile or immobilised support comprising one or more functional groups independently selected from 1,3-ketoesters, 1,3-ketothioesters or 1,3-ketoamides to form a covalently bound support-biomolecule compound, wherein the 1,3-ketoester, 1,3-ketothioester or 1,3-ketoamide is a group of Formula 1: Wherein R 1 is an optionally substituted hydrocarbyl, perhalogenated hydrocarbyl, or a heterocyclyl group; Y is hydrogen, an optionally substituted hydrocarbyl, or a heterocyclyl group; X is —O, —NR 2 or —S, wherein the free valence of —O, —NR 2 or —S is bonded to the support optionally via a linker; and R 2 is hydrogen, an optionally substituted hydrocarbyl, or a heterocyclyl group; wherein the biomolecule is selected from the group consisting of: antibodies, antibody fragments, modified antibodies, antibody-drug conjugates, enzymes, proteins, peptides, polypeptides, modified peptides, peptide nucleic acids (PNAs), metalloproteins, peptide-drug conjugates, peptide-oligonucleotide hybrids, amino acids, non-naturally occurring amino acids, diamino acids, synthetic amino acids, amino acid-drug conjugates, oligonucleotides, modified oligonucleotides, oligonucleotides-drug conjugates, nucleotides, nucleosides, purines, pvrimidines, oligosaccharides, polysaccharide, disaccharides, monosaccharides, amino sugars, lipids, phospholipids, glycolipids, sterols, vitamins, hormones, neurotransmitters, carbohydrates, sugars, viruses, cells and active pharmaceutical ingredients (APIs).