Patent ID: 9629357
Date: 2017-04-25
CPC Classifications: A01N

Claim:
1. A method of clinical processing and cryopreservation of a cell-containing sample, the method comprising: obtaining testicular tissue from an individual, suspending the testicular tissue, or cells obtained from the tissue, in 0.5 volume of a first cryopreservation medium comprising 10% HSA and PBS; and adding 0.5 volume of a second cryopreservation medium comprising PBS, 1% dextran, and 10% DMSO to the cells or tissue in the first cryopreservation medium, wherein the second cryopreservation medium is added drop wise over the course of 0.5 to 10 minutes; placing the cryopreservation medium containing the cells or tissue in a cryopreservation vial; cooling the vial to 4° C. and holding the vial at 4° C. for a period of time; cooling the vial at a rate of −1° C. per minute to a temperature of −80° C.; cooling the vial at a rate of −50° C. per minute to a temperature of −140° C.; holding the vial at −140° C. for a period of time between 0.1 and 60 minutes; and transferring the vial to the vapor phase of liquid nitrogen.