Patent ID: 9651558
Date: 2017-05-16
CPC Classifications: C07K,C12N,G01N

Claim:
1. A Gram-positive host cell capable of post-translationally modifying a polypeptide of interest to a dehydroresidue or thioether bridge containing polypeptide, wherein the host cell comprises an expression vector comprising a nucleic acid construct encoding a fusion peptide comprising, from the N-terminus to the C-terminus, at least the following sequential elements: (a) an N-terminal lantibiotic leader sequence that can be recognized by a lantiobiotic dehydratase and a lantibiotic cyclase; (b) an amino acid sequence of interest to be post-translationally modified to a dehydroresidue- or thioether-bridge containing polypeptide; (c) a hydrophilic cell-wall spanning domain; (d) a sortase recognition motif; (e) a hydrophobic membrane spanning domain; and (f) a C-terminal charged membrane anchoring domain and wherein the host cell is a lactic bacterium that contains lantibiotic biosynthesis enzymes and a lantiobiotic transporter which exports the post-translationally modified polypeptide out of the host cell.