Patent ID: 9657341
Date: 2017-05-23
CPC Classifications: B01L,C12Q

Claim:
1. A microfluidic system for analyzing nucleic acid, the microfluidic system comprising: a reagent supply device including a sample chamber into which a sample can be injected, one or more reagent chambers for containing one or more reagents for extracting nucleic acid from the sample, and a waste chamber in which the used reagent can be discarded; a binding-lysis chamber in which cells are captured from the sample and lysed to form a cell lysate containing nucleic acid; a plurality of particles for cell binding disposed in the binding-lysis chamber; a plurality of rehydration chambers into which the cell lysate formed in the binding-lysis chamber can be distributed and mixed with a nucleic acid amplification reagent to form an amplification reaction mixture; a plurality of amplification chambers in which a nucleic acid amplification reaction is performed on the amplification reaction mixture introduced from the plurality of rehydration chambers; and a flow channel system including an outlet and a plurality of inlets connected to the reagent supply device and forming an integrated fluid flow between the binding-lysis chamber, the rehydration chambers, and the amplification chambers, and further comprising a fluid flow part comprising: a membrane part comprising an elastic membrane bonded to the bottom surface of the fluid flow part to form bottom surfaces of the binding-lysis chamber and the plurality of rehydration chambers; a pneumatic part bonded to a bottom surface of the membrane part and having a plurality of ports for applying pneumatic pressure to one or more predetermined positions of the membrane part; and a guide part for installing the reagent supply device, wherein the guide part is disposed on an upper portion of the fluid flow part and configured to align the plurality of inlets and the outlet of the fluid flow part with the plurality of chambers of the reagent supply part.