Patent ID: 9574190
Date: 2017-02-21
CPC Classifications: A61K,C07K,C12N,G01N

Claim:
1. A screening method for selecting peptides that consist of 3 to 50 amino acids and bind to a target molecule at a first pH and do not bind to the target molecule at a second pH, comprising: synthesizing a nucleic acid library in which nucleic acids respectively encode peptides containing at least one special amino acid that undergoes a pH-dependent change in the charge of the side chain thereof; using the nucleic acid library to express the peptides and prepare a peptide library; bringing the peptide library into contact with the target molecule and incubating them under the first pH condition and selecting peptides that bind to the target molecule; and selecting, from the peptides that have bound to the target molecule, peptides that do not bind to the target molecule under the second pH condition.