Patent ID: 9732382
Date: 2017-08-15
CPC Classifications: C12N,C12Q,G01N

Claim:
1. A method for protecting an enzyme from photo-induced damage in an illuminated reaction, the method comprising: providing a reaction mixture comprising the enzyme, and a fluorescent or fluorogenic substrate for the enzyme, wherein interaction of the enzyme and the fluorescent or fluorogenic substrate under excitation illumination results in altered activity of the enzyme; adding a photoprotective agent to the reaction mixture, wherein the photoprotective agent comprises a compound of a formula selected from the group consisting of:  and a carboxylate salt thereof; and illuminating the reaction mixture with an excitation illumination, wherein the photoprotective agent reduces an amount of photo-induced damage to the enzyme resulting from interaction of the enzyme with the fluorescent or fluorogenic substrate under the excitation illumination to an amount that is less than that which would occur in the absence of the photoprotective agent.