Patent ID: 9725698
Date: 2017-08-08
CPC Classifications: A61K,A61P,C12N

Claim:
1. A method of producing human trophoblast-derived mesenchymal stem cells (T-MSCs) from human embryonic stem cells (hESCs) or induced pluripotent stem cells (iPSCs), the method comprising: (a) culturing hESCs or iPSCs in a medium comprising bone morphogenetic protein-4 (BMP-4), and optionally a TGFβ inhibitor, for a first time period of 1 to 5 days sufficient for the hESCs or iPSCs to differentiate into trophoblast cells; (b) dissociating the trophoblast cells into single trophoblast cells; and (c) plating the single trophoblast cells from step (b) onto gelatin, vitronectin, laminin, fibronectin, Matrigel or collagen-coated plates, and culturing said single trophoblast cells for a second time period of 4 to 10 days in a mesenchymal stem cell (MSC) growth medium containing LIF, bFGF or PDGF, or a combination thereof, thereby producing human T-MSCs.