Patent ID: 9719107
Date: 2017-08-01
CPC Classifications: C12N

Claim:
1. An Adenovirus packaging cell line permissive for replication of a fully-deleted Adenoviral vector independent of helper Adenovirus, the cell line comprising an Adenovirus early region 1 (E1) coding sequence and an Adenovirus pIX coding sequence, both of which are stably integrated into the cell line; and further comprising a replication defective circular packaging construct having at least a subset of Adenoviral late genes selected from the group consisting of L1, L2, L3, L4, L5, E2A, and E4, while being absent of at least one inverted terminal repeat and a packaging signal; wherein upon transfection of a fully-deleted Adenoviral vector construct into the cell line, a fully-deleted adenoviral-based gene transfer vector can be encapsidated independent of helper Adenovirus, wherein the encapsidated fully-deleted adenoviral-based gene transfer vector includes both adenoviral inverted terminal repeats, the packaging signal, and at least one DNA insert encoding a protein of interest, and wherein the encapsidated fully-deleted adenoviral-based gene transfer vector is absent of adenoviral structural genes.