Patent ID: 9624536
Date: 2017-04-18
CPC Classifications: B01L,C12Q

Claim:
1. A microfluidic chip for use in multiplexed analysis of samples, the microfluidic chip comprising at least three stacked fluidic layers, the microfluidic chip further comprising a plurality of sensing chambers comprising at least a plurality first of sensing chambers, a first plurality of fluid supply channels, each of the fluid supply channels being configured to provide a different fluid, the first plurality of fluid supply channels comprising a first plurality of microfluidic channels, the first plurality of microfluidic channels comprising at least wherein each sensing chamber of the plurality of first sensing chambers is fluidly connected to the first supply channel through one of the first microfluidic channels, and is fluidly connected to the first supply channel through one of the second microfluidic channels, and wherein each of the first sensing chambers is disposed in a fluidic layer of the microfluidic chip that is different than the first one of the one or more fluidic layers in which the corresponding first microfluidic channel is disposed, is different than the first one of the one or more fluidic layers in which the corresponding second microfluidic channel is disposed, is different than the fluidic layer in which the first fluid supply channel is disposed, and is different than the fluidic layer in which the second fluid supply channel is disposed, the fluid supply channels and the microfluidic channels thus forming a hierarchical delivery distribution system for supplying the fluids to the plurality of sensing chambers.