Patent ID: 9644217
Date: 2017-05-09
CPC Classifications: C12N,C12P

Claim:
1. A method for production of retinoid from a microorganism, comprising: culturing a microorganism having retinoid producing efficacy in a medium containing a lipophilic substance; and isolating said retinoid from said lipophilic substance without degrading a cell wall of said microorganism; wherein said microorganism transformed with: a gene encoding acetyl-CoA acetyl transferase/hydroxymethylglutaryl(HMG)-CoA reductase comprising the amino acid sequence of SEQ ID NO: 1 from a gene encoding HMG-CoA synthase comprising the amino acid sequence of SEQ ID NO: 2 from a gene encoding mevalonate kinase comprising the amino acid sequence of SEQ ID NO: 3 from a gene encoding phosphomevalonate kinase comprising the amino acid sequence of SEQ ID NO: 4 from a gene encoding mevalonate diphosphate decarboxylase comprising the amino acid sequence of SEQ ID NO: 5 from a gene encoding isopentinyl diphosphate (IPP) isomerase comprising the amino acid sequence of SEQ ID NO: 6 from a gene encoding geranylgeranyl pyrophosphate (GGPP) synthase comprising the amino acid sequence of SEQ ID NO: 7 from a gene encoding phytoene synthase comprising the amino acid sequence of SEQ ID NO:8 from a gene encoding phytoene dehydrogenase comprising the amino acid sequence of SEQ ID NO: 9 from a gene encoding lycopene β-cyclase comprising the amino acid sequence of SEQ ID NO: 10 from