Patent ID: 9605258
Date: 2017-03-28
CPC Classifications: C12N

Claim:
1. A method for modifying the production of a polypeptide of interest in a cell of an organism, the method comprising: (a) selecting an mRNA encoding a polypeptide of interest, comprising: (b) mutating the nucleotide sequence of the mRNA of (a) to produce a mutated mRNA having an enhanced or reduced MFE RNA secondary structure as compared to the MFE RNA secondary structure of the nucleotide sequence of the mRNA of (a)); wherein the mutated mRNA encodes a mutated polypeptide sequence as compared to the polypeptide sequence encoded by the mRNA of (a), the mutated polypeptide sequence retains the function of the polypeptide encoded by the mRNA of (a), and expression of the mutated mRNA sequence results in modified production of the polypeptide of interest as compared to the wild-type mRNA sequence.