Patent ID: 9682109
Date: 2017-06-20
CPC Classifications: A61K,A61P,C12N,C12Q

Claim:
1. A method of detecting an Hepatitis B Virus (HBV) variant from an HBV isolate, said method comprising: isolating the DNA genome or the corresponding mRNA genome from said HBV isolate; amplifying a portion of the genome, or cDNA from the mRNA, with one or more biotin-labeled or ligand-labeled primers that selectively bind to a portion of the HBV reverse transcriptase (rt) gene which codes for a threonine (T) at rt85 and an alanine (A), an isoleucine (I) or a valine (V) at rt204, creating labeled amplification products, wherein the one or more primers do not bind to the portion of the HBV rt gene if the HBV rt gene does not encode for the combination of the T at rt85 and the A, the I or the V at rt204, capturing the labeled amplification products by hybridization with an oligonucleotide that binds to the biotin or to the ligand, wherein the oligonucleotide is immobilized on a solid support, sequencing the amplification products, and determining whether or not the HBV isolate included a T at rt85 and an A, I, or V at rt 204.