Patent ID: 9561270
Date: 2017-02-07
CPC Classifications: A61K,A61P,C12N

Claim:
1. A method of producing an antigenic composition comprising as at least one first component a porcine circovirus type 2 open reading frame 2 (PCV-2 ORF2)-antigen and having reduced virucidal activity, said method comprising the steps: a) obtaining a first liquid containing recombinantly expressed ORF2 antigen of PCV-2, inactivated baculovirus vector, a neutralizing agent, and cell culture media used for the production of recombinant proteins in cultivated host cells; b) removing at least a portion of the first liquid containing the PCV-2 ORF2 antigen by an at least one exchange of the portion of the first liquid against a second liquid, wherein the second liquid is different from the first liquid and is a non-virucidal pharmaceutically acceptable buffer selected from the group consisting of a saline buffer, phosphate buffer, or combinations thereof and wherein the at least one exchange of the portion of the first liquid against a second liquid comprises the steps of: i) adding the second liquid to the first liquid; and ii) concentrating the PCV-2 ORF2 antigen 3-fold to 50-fold by filtration through a semi-permeable membrane with an average pore size of 50 kDa to 500 kDa and removing a portion of the first and second liquids whereby the PCV-2 ORF2 antigenic composition produced has reduced virucidal activity as compared to the first liquid; wherein the reduced virucidal activity of the PCV-2 ORF2 antigenic composition can be determined by adding an additional live antigen from at least one other disease-causing organism in swine to the PCV-2 ORF2 antigenic composition and comparing the virucidal activity of the PCV-2 ORF2 antigenic composition on the additional live antigen of the PCV-2 ORF2 antigenic composition with the virucidal activity of a PCV-2 ORF2 antigenic composition having the same additional live antigen as the PCV-2 ORF2 antigenic composition but that has not undergone steps a) and b) and wherein reduced virucidal activity is defined as causing a loss of less than 0.7 log TCID