Patent ID: 9637777
Date: 2017-05-02
CPC Classifications: B01J,C12Q

Claim:
1. A method of preventing a significant reduction in duplexes detectable in a hybridization assay, the method comprising: (i) selecting probe lengths for sets of oligonucleotide probes, wherein probes comprise different subsequences such that at least one subsequence is complementary to a subsequence in a cognate target; wherein probes for longer cognate targets are longer in length than probes for shorter cognate targets; (ii) selecting, for each set of probes, a density of probes attached per unit area on a solid phase carrier which is below a limit at which said significant reduction in detectable duplexes is predicated to take place; (iii) producing said probes and affixing said probes to different solid phase carriers at the selected density; and (iv) annealing targets to the probes, wherein signal intensities of probes and targets of different lengths are about the same.