Patent ID: 9688972
Date: 2017-06-27
CPC Classifications: C12N,C12Q,C12Y

Claim:
1. A method of modifying a target nucleic acid molecule comprising: contacting the target nucleic acid molecule having a target sequence with (i) a single polynucleotide comprising (a) a targeting region that comprises deoxyribonucleic acid (DNA) or ribonucleic acid (RNA) and configured to hybridize to the target sequence; and (b) an activating region adjacent to the targeting region that comprises DNA and wherein the activating region comprises a stem loop structure; and (ii) a Cpf1, wherein the Cpf1 binds with the activating region of the single polynucleotide, wherein the contacting of the target nucleic acid molecule with the single polynucleotide and the Cpf1 occurs (A) in vitro, (B) in a non-human cell, or (C) in an isolated cell and wherein the target nucleic acid molecule is cleaved.