Patent ID: 9617587
Date: 2017-04-11
CPC Classifications: C12Q

Claim:
1. A method for determining the presence, absence or amount of a target sequence in sample nucleic acid, comprising: a) amplifying a target sequence in the sample nucleic acid, wherein:  the target sequence comprises a first strand and a second strand,  the first strand and second strand are complementary to each other, and  the amplifying comprises contacting non-denatured sample nucleic acid under cleavage agent-free, helicase-free and recombinase-free isothermal amplification conditions with: b) detecting the nucleic acid amplification product, wherein detecting the nucleic acid amplification product comprises use of a real-time detection method and is performed in 10 minutes or less from the time the sample nucleic acid is contacted with (a)(i) and (a)(ii), whereby the presence, absence or amount of a target sequence in sample nucleic acid is determined.