Patent ID: 9611455
Date: 2017-04-04
CPC Classifications: C07K,C12N,C12P

Claim:
1. A method for the generation of an elevated pH-tolerant lepidopteran insect cells comprising: (a) culturing a population of cells from a lepidopteran insect cell line in a standard culture medium that supports the growth of insect cells and has a pH of from about 6.0 to about 6.4; (b) culturing the cells in elevated pH culture medium that has a pH that is greater than the pH of the standard culture medium in step (a); and (c) allowing the cells to adapt to the elevated pH culture medium, wherein the adapted cells exhibit similar growth characteristics at a pH greater than or equal to 7.0 to a population of cells of the insect cell line cultured in the standard culture medium.