Patent ID: 9580689
Date: 2017-02-28
CPC Classifications: A01K,C07K,C12N

Claim:
1. A method to increase efficiency of reprogramming cells to form induced pluripotent stem cells (iPSC) comprising a) introducing i) a nucleic acid encoding a fusion protein comprising a transactivation domain from MyoD fused to the N-terminus or C-terminus of Oct4 operably linked to a promoter, and ii) at least one nucleic acid encoding Sox2, Klf4, and optionally c-Myc operably linked to a promoter into a somatic cell, wherein the somatic cell expresses the nucleic acids of i) and ii); and b) culturing said somatic cell for a time to reprogram into a pluripotent stein cell, wherein the fusion protein increases the efficiency of reprogramming the somatic cell into a iPSC.