Patent ID: 9644187
Date: 2017-05-09
CPC Classifications: C12N

Claim:
1. A method of preparing a high purity virus stock solution, wherein the virus stock solution comprises a protein concentration of less than 100 fg/TCID 50 suitable for validation of retentive filters, the method comprising the sequential steps of: (a) harvesting the culture supernatant of virus-infected cells grown under low serum conditions, wherein the culture supernatant is harvested after virus-induced cell lysis, thereby obtaining a viral sample; (b) concentrating the viral sample by ultrafiltration, tangential flow filtration, dialysis, or a combination of the foregoing, and exchanging the culture supernatant for a suitable buffer; and (c) precipitating the virus in the viral sample by ultracentrifugation, chemical-induced precipitation, chromatographic binding, polymer-induced aggregation, or a combination thereof, wherein the precipitated virus comprises a protein concentration of less than 100 fg/TCID thereby preparing a high purity virus stock solution suitable for validation of retentive filters.