Patent ID: 9708596
Date: 2017-07-18
CPC Classifications: A61P,C12N,C12Y

Claim:
1. A method for preparing a polypeptide mutant of the Bordetella pertussis CyaA protein comprising: a) substituting the glutamic acid residue at position 570 of SEQ ID NO: 1 with another amino acid residue selected from the group consisting of Gln, Asn, Met, Thr, Ser, Gly, Arg, Lys, Val, Leu, Cys, Ile, and Asp; and b) substituting the lysine residue at position 860 of SEQ ID NO: 1 with another amino acid residue selected from the group consisting of Gln, Asn, Met, Thr, Ser, Gly, Arg, Val, Leu, Cys, and Ile, to generate the polypeptide mutant of the