Patent ID: 9598453
Date: 2017-03-21
CPC Classifications: C07D,C07H,C12N,C12Q

Claim:
1. A method for modifying a nucleic acid, said method comprising: (a) performing a first exchange reaction comprising contacting: a nucleic acid comprising an aldehyde group, and a bifunctional linking reagent comprising a hydrazine or amine moiety under conditions to form an imine bond coupling the nucleic acid to the bifunctional linking reagent; wherein the bifunctional linking reagent further comprises a functional tag; (b) contacting the sample with a first binding partner to the first functional tag; and (c) performing a first purification step comprising separating the first binding partner from the sample, thereby isolating a nucleic acid from a sample; (d) performing a second exchange reaction comprising contacting the nucleic acid isolated in step (c) with an exchange reagent under conditions sufficient for the imine bond to be broken between the nucleic acid and the bifunctional linking reagent and for a new imine bond to form between the exchange reagent and the nucleic acid.