Patent ID: 9587276
Date: 2017-03-07
CPC Classifications: B01L,C12Q,G01N,G02B

Claim:
1. A method of performing a sequencing by synthesis reaction, the method comprising: a) providing a substrate comprising: b) immobilizing an individual polymerase/template/primer complex within one of the apertures sufficiently proximal to the first optical waveguide to be illuminated by the evanescent field; c) passing excitation light through the first optical waveguide, thereby generating the evanescent field; d) exposing the polymerase/template/primer complex to a set of labeled nucleotides or nucleotide analogs under conditions that promote nascent strand synthesis; and e) detecting emissions from said one of the apertures, wherein said emissions are emitted from the labeled nucleotides or nucleotide analogs during said nascent strand synthesis, and are indicative of a sequence being synthesized, thereby performing a sequencing by synthesis reaction.