Patent ID: 9538745
Date: 2017-01-10
CPC Classifications: A01N

Claim:
1. A method for cryopreserving a mammalian cell, the method comprising: (a) suspending said cell in a vitrification solution comprising one or more cryoprotective agents, wherein a concentration of the combination of all cryoprotective agents in said vitrification solution is less than or equal to 4 M; (b) placing said cell in said vitrification solution in a microcapillary tube, wherein said microcapillary tube has a wall made of a material having a thermal conductivity of at least 0.2 W m (c) cooling said cell in said vitrification solution in said microcapillary tube to a temperature less than or equal to a glass transition temperature of said vitrification solution at a rate greater than 200,000° C./min, wherein said cooling causes vitrification of said cell.