Patent ID: 9714419
Date: 2017-07-25
CPC Classifications: A61K,A61P,C12N,C12Y

Claim:
1. A PEGylated tyrosyl-tRNA synthetase (YRS) polypeptide selected from, (a) a YRS polypeptide consisting of a sequence that is at least 95% identical to the sequence set forth in SEQ ID NO:7 YRS(1-353), which is modified by A4C, C67S, and C250S substitutions, where a maleimide monomethoxy polyethylene glycol (mPEG) derivative of about 40,000 Daltons is covalently attached via a thio ether linkage to A4C; (b) a YRS polypeptide consisting of a sequence that is at least 98% identical to the sequence set forth in SEQ ID NO:7 YRS(1-353), which is modified by A4C, C67S, and C250S substitutions, where a maleimide monomethoxy polyethylene glycol (mPEG) derivative of about 40,000 Daltons is covalently attached via a thio ether linkage to A4C; (c) a YRS polypeptide consisting of the sequence set forth in SEQ ID NO:7 YRS(1-353), which is modified by A4C, C67S, and C250S substitutions, where a maleimide monomethoxy polyethylene glycol (mPEG) derivative of about 40,000 Daltons is covalently attached via a thio ether linkage to A4C; (d) a YRS polypeptide consisting of a sequence that is at least 95% identical to the sequence set forth in SEQ ID NO:7 YRS(1-353), which is modified by C67S, C250S, and A351C substitutions, where a maleimide monomethoxy polyethylene glycol (mPEG) derivative of about 40,000 Daltons is covalently attached via a thio ether linkage to A351C; (e) a YRS polypeptide consisting of a sequence that is at least 98% identical to the sequence set forth in SEQ ID NO:7 YRS(1-353), which is modified by C67S, C250S, and A351C substitutions, where a maleimide monomethoxy polyethylene glycol (mPEG) derivative of about 40,000 Daltons is covalently attached via a thio ether linkage to A351; and (f) a YRS polypeptide consisting of the sequence set forth in SEQ ID NO:7 YRS(1-353), which is modified by C67S, C250S, and A351C substitutions, where a maleimide monomethoxy polyethylene glycol (mPEG) derivative of about 40,000 Daltons is covalently attached via a thio ether linkage to A351C.