Patent ID: 9534246
Date: 2017-01-03
CPC Classifications: C12P,C12Q,G01N

Claim:
1. A method for selecting one or more mammalian cells expressing secreted antibody for use as a biopharmaceutical, the method comprising the steps of: (a) providing a population of mammalian cells in a medium free from animal-derived components, wherein the cell population comprises at least one cell expressing said secreted antibody; (b) contacting the cell population with a label which directly binds to said antibody on the surface of the cell; (c) detecting said label bound to the antibody on the surface of said cell; and (d) selecting said cell based on the presence of said label bound to the antibody on the surface of said cell; wherein the label is selected from the group consisting of Protein A, Protein G or Protein L; and whereby one or more mammalian cells expressing a secreted antibody for use as a biopharmaceutical is selected.