Patent ID: 9567576
Date: 2017-02-14
CPC Classifications: C12N,C12P,C12Y,Y02E,Y02P

Claim:
1. An isolated polynucleotide encoding a mutant fermentation respiration switch (FrsA) protein, wherein the mutant FrsA protein has pyruvate decarboxylase activity, and wherein the mutant FrsA protein comprises the amino acid sequence of SEQ ID NO: 2, except that: (1) cysteine at the position corresponding to residue 131 of SEQ ID NO: 2 is replaced with alanine in the mutant FrsA protein, or (2) amino acids corresponding to residues 2-19 of SEQ ID NO: 2 are deleted and cysteine at the position corresponding to residue 131 of SEQ ID NO: 2 is replaced with alanine in the mutant FrsA protein.