Patent ID: 9659759
Date: 2017-05-23
CPC Classifications: G01N,G16C,H01J

Claim:
1. A method for quantitatively determining structural isomers comprising: a) subjecting an equimolar mixture containing the reference isomers to ionization and selecting precursor ions having a mass to charge ratio in an m/z range between 1 to 40000 (z=1) followed by fragmentation to generate product ions that are both common and unique in nature to the reference isomers; b) selecting product ions that are common to the isomer pairs to normalize the unique product ions followed by measuring the peak area/peak intensity ratios of the unique and common product ions to generate equimolar concentration response curves for each individual isomer pair as a function of equimolar ratios; and c) subjecting a sample containing unknown quantities of the isomers/isomer pairs in context to ionization and selecting precursor ions having a mass to charge ratio in an m/z range between 1 to 40000 (z=1) followed by fragmentation to generate product ions that are both common and unique in nature as in step (a); measuring the unique to common product ion ratio(s), and determining the corresponding equimolar ratio(s) of the isomer pairs present based on the equimolar concentration response curve(s) generated in step (b).