Patent ID: 9695426
Date: 2017-07-04
CPC Classifications: C12N,C12P,Y02E

Claim:
1. A recombinant microorganism selected from a genus of Escherichia, Corynebacterium, Lactobacillus, Lactococcus, Salmonella, Enterobacter, Enterococcus, Erwinia, Pantoea, Morganella, Pectobacterium, Proteus, Serratia, Shigella, Klebsiella, Citrobacter, Saccharomyces, Dekkera, Kluyveromyces , and Pichia that produces an alcohol selected from the group consisting of: (a) 1-propanol, (b) 1-butanol, and (c) 2-methyl 1-butanol, wherein the alcohol is produced from a metabolite comprising 2-ketobutyrate; wherein the recombinant microorganism comprises a recombinant citramalate synthase (cimA) having at least 95% identity to SEQ ID NO:89 and having citramalate synthase activity; wherein the recombinant microorganism comprises a 2-keto-acid decarboxylase having at least 95% identity to SEQ ID NO:28, 32, 34 or 36 and having 2-keto-acid decarboxylase activity; wherein the recombinant microorganism comprises a recombinant thrA*, a recombinant thrB from E. coli , and a recombinant thrC from E. coli , wherein the recombinant thrA* is a feedback-resistant mutant of thrA from E. Coli , and wherein the recombinant thrA* encodes a homoserine dehydrogenase, the recombinant thrB encodes a homoserine kinase and the recombinant thrC encodes a threonine synthase; and wherein the recombinant microorganism is recombinantly engineered to express or overexpress the citramalate synthase, the 2-keto-acid decarboxylase, the homoserine dehydroqenase, the homoserine kinase, and the threonine synthase as compared to a parental microorganism lacking said engineering when grown under the same conditions and wherein: (i) the microorganism expresses an isopropylmalate isomerase (LeuCD) having at least 95% identity to SEQ ID NO:54 and 56, an isopropylmalate dehydrogenase (LeuB) having at least 90% identity to SEQ ID NO:52; a 2-isopropylmalate synthase (LeuA) having at least 95% identity to SEQ ID NO:50; and an alcohol dehydrogenase (Adh2) having at least 90% identity to SEQ ID NO:38, for production of 1-butanol; (ii) the microorganism expresses an isopropylmalate isomerase (LeuCD) having at least 95% identity to SEQ ID NO:54 and 56, an isopropylmalate dehydrogenase (LeuB) having at least 90% identity to SEQ ID NO:52, and an alcohol dehydrogenase (Adh2) having at least 90% identity to SEQ ID NO:38, for production of 1-propanol; or (iii) the microorganism expresses an isopropylmalate isomerase (LeuCD) having at least 95% identity to SEQ ID NO:54 and 56, an isopropylmalate dehydrogenase (LeuB) having at least 90% identity to SEQ ID NO:52, an acetohydroxy acid synthase (IlvHI) having at least 95% identity to SEQ ID NO:40 and 42, an acetohydroxy acid isomeroreductase (IlvC) having at least 95% identity to SEQ ID NO:44 and an alcohol dehydrogenase (Adh2) having at least 90% identity to SEQ ID NO:38, for production of 2-methyl 1-butanol.