Patent ID: 9550985
Date: 2017-01-24
CPC Classifications: B01L,C12N,C12Q,G01N,G02B,Y10T

Claim:
1. A method for quantifying an unamplified double stranded target nucleic acid in a sample fluid containing or suspected of containing the unamplified double stranded target nucleic acid, comprising: combining in a microfluidic channel located in a biochip, said biochip further comprising a plurality of microfluidic channels and active areas for sample manipulation, at least a portion of the sample fluid and a probe fluid containing a binding agent comprising a signaling moiety to form a test fluid; heating said test fluid to denature the unamplified double stranded target nucleic acid and cooling said test fluid to bind the binding agent to the denatured unamplified target nucleic acid; maintaining the test fluid stationary in the microfluidic channel at a detector region; detecting the signaling moiety in the test fluid at said detector region; and quantifying the denatured unamplified target nucleic acid in the test fluid based on the detected signaling moiety; wherein the denatured unamplified target nucleic acid in the test fluid is quantified within 1 minute of combining the portion of the sample fluid and said probe fluid.