Patent ID: 9587219
Date: 2017-03-07
CPC Classifications: C12M,C12N,Y10T

Claim:
1. A method for producing a cell culture vessel comprising a container section in which an inner chamber for accommodating cells and media is provided, the container section comprising a cell support film, which is at least provided with a film substrate layer and, disposed thereon, a stimuli-responsive polymer layer having a cell-adhesive surface capable of changing into a non-cell-adhesive surface upon reception of a particular stimulus, fixed to at least a part of the inner wall surface facing the inner chamber in a manner such that the stimuli-responsive polymer layer is disposed facing the inner chamber, the method comprising: a step of cutting a long-sized cell support film to prepare a sheet-like cell support film; a step of cell support film fixation comprising fixing a sheet-like cell support film to a first member of the container section having a region of an opened inner wall surface to which the cell support film is fixed, thereby producing a film-fixed first member; a step of member bonding for constituting the container section by bonding the film-fixed first member to one or more other members; and a step of sterilization by irradiation of the cell culture vessel with a γ beam under vacuum conditions.