Patent ID: 9719076
Date: 2017-08-01
CPC Classifications: B01D,C07K,C12N,C12P,C12R,C12Y

Claim:
1. An animal protein free (APF) chromatographic system for obtaining a biologically active botulinum neurotoxin, the system comprising: a first apparatus for culturing Clostridium botulinum bacteria, the first apparatus capable of containing an APF culture medium; a second apparatus for fermenting Clostridium botulinum bacteria which have been cultured in the first apparatus, the second apparatus capable of containing an APF fermentation medium; a third apparatus for harvesting the fermentation medium; a fourth apparatus for concentrating the harvested fermentation medium; a fifth apparatus for carrying out a first purification of the botulinum neurotoxin from the harvested fermentation medium, wherein the fifth apparatus comprises an anion exchange chromatography media, thereby obtaining a first purified botulinum neurotoxin; a sixth apparatus for carrying out a second purification of the first purified botulinum neurotoxin wherein the sixth apparatus comprises a cation exchange chromatography media, thereby obtaining a second purified botulinum neurotoxin, and a seventh apparatus for carrying out a third purification of the second purified botulinum neurotoxin, thereby obtaining a third purified botulinum neurotoxin.