Patent ID: 9725714
Date: 2017-08-08
CPC Classifications: A61K,A61P,C12N,C12Q

Claim:
1. A method for detecting if two complexes are in proximity to one another comprising: contacting a first target nucleic acid with a first complex, wherein the first complex comprises a first site-directed polypeptide, a first modified nucleic acid-targeting nucleic acid comprising a first nucleic acid sequence complementary to the first target nucleic acid, and a first effector protein, wherein the first effector protein (i) is adapted to bind to the first modified nucleic acid-targeting nucleic acid, and (ii) comprises a first non-native sequence that comprises a first portion of an endonuclease split system; contacting a second target nucleic acid with a second complex, wherein the second complex comprises a second site-directed polypeptide, a second modified nucleic acid-targeting nucleic acid comprising a second nucleic acid sequence complementary to the second target nucleic acid, and a second effector protein, wherein the second effector protein (i) is adapted to bind to the second modified nucleic acid-targeting nucleic acid, and (ii) comprises a second non-native sequence that comprises a second portion of an endonuclease split system; and detecting an interaction between the first portion and the second portion, wherein (i) the split system forms an active endonuclease when the first portion of the endonuclease split system and the second portion of the endonuclease split system are in proximity, (ii) the interaction results in nucleic acid cleavage by the active endonuclease, and (iii) the detecting indicates the first complex and the second complex are in proximity to one another.