Patent ID: 9580752
Date: 2017-02-28
CPC Classifications: C12Q,G01N

Claim:
1. A method of treating ulcerative colitis (UC) in a human subject, comprising: obtaining a sample from the human subject with UC; contacting the sample with an oligonucleotide probe specific to an “A” allele at nucleotide 465 of SEQ ID NO:1, an oligonucleotide probe specific to an “A” allele at nucleotide 301 of SEQ ID NO:2, an oligonucleotide probe specific to a “C” allele at nucleotide 301 of SEQ ID NO:3, an oligonucleotide probe specific to an “A” allele at nucleotide 3412 of SEQ ID NO:4, an oligonucleotide probe specific to a variant allele at any one of nucleotides 4505-4604 of SEQ ID NO:4, an oligonucleotide probe specific to a “G” allele at nucleotide 364 of SEQ ID NO:5, an oligonucleotide probe specific to an “A” allele at nucleotide 251 of SEQ ID NO:6, an oligonucleotide probe specific to a “G” allele at nucleotide 239 of SEQ ID NO:7, an oligonucleotide probe specific to an “A” allele at nucleotide 250 of SEQ ID NO:8, an oligonucleotide probe specific to a “G” allele at nucleotide 501 of SEQ ID NO:9, an oligonucleotide probe specific to a “G” allele at nucleotide 301 of SEQ ID NO:10, an oligonucleotide probe specific to an “A” allele at nucleotide 501 of SEQ ID NO:11, an oligonucleotide probe specific to an “A” allele at nucleotide 501 of SEQ ID NO:12, an oligonucleotide probe specific to a “G” allele at nucleotide 201 of SEQ ID NO:13, an oligonucleotide probe specific to an “A” allele at nucleotide 244 of SEQ ID NO:14, an oligonucleotide probe specific to a “G” allele at nucleotide 501 of SEQ ID NO:15, an oligonucleotide probe specific to a “G” allele at nucleotide 195 of SEQ ID NO:16, an oligonucleotide probe specific to a “G” allele at nucleotide 101 of SEQ ID NO:17, an oligonucleotide probe specific to an “A” allele at nucleotide 582 of SEQ ID NO:18, an oligonucleotide probe specific to an “A” allele at nucleotide 301 of SEQ ID NO:19, an oligonucleotide probe specific to a “G” allele at nucleotide 324 of SEQ ID NO:20, an oligonucleotide probe specific to an “A” allele at nucleotide 301 of SEQ ID NO:21, an oligonucleotide probe specific to a “G” allele at nucleotide 1394 of SEQ ID NO:22, an oligonucleotide probe specific to an “A” allele at nucleotide 251 of SEQ ID NO:23, an oligonucleotide probe specific to an “A” allele at nucleotide 201 of SEQ ID NO:24, an oligonucleotide probe specific to an “A” allele at nucleotide 301 of SEQ ID NO:25, an oligonucleotide probe specific to a “G” allele at nucleotide 301 of SEQ ID NO:26, an oligonucleotide probe specific to a “G” allele at nucleotide 1124 of SEQ ID NO:27, an oligonucleotide probe specific to an “A” allele at nucleotide 501 of SEQ ID NO:28, an oligonucleotide probe specific to a “G” allele at nucleotide 2000 of SEQ ID NO:29, an oligonucleotide probe specific to a “G” allele at nucleotide 351 of SEQ ID NO:30, an oligonucleotide probe specific to an “A” allele at nucleotide 301 of SEQ ID NO:31, an oligonucleotide probe specific to a “G” allele at nucleotide 380 of SEQ ID NO:32, an oligonucleotide probe specific to an “A” allele at nucleotide 201 of SEQ ID NO:33, an oligonucleotide probe specific to a “G” allele at nucleotide 1158 of SEQ ID NO:34, an oligonucleotide probe specific to a “G” allele at nucleotide 371 of SEQ ID NO:35, an oligonucleotide probe specific to a “C” allele at nucleotide 201 of SEQ ID NO:36, an oligonucleotide probe specific to an “A” allele at nucleotide 501 of SEQ ID NO:37, an oligonucleotide probe specific to a “G” allele at nucleotide 50 of SEQ ID NO:38, an oligonucleotide probe specific to an “A” allele at nucleotide 201 of SEQ ID NO:39, an oligonucleotide probe specific to an “A” allele at nucleotide 501 of SEQ ID NO:40, an oligonucleotide probe specific to a “C” allele at nucleotide 201 of SEQ ID NO:41, an oligonucleotide probe specific to a “G” allele at nucleotide 401 of SEQ ID NO:42, an oligonucleotide probe specific to a “C” allele at nucleotide 401 of SEQ ID NO:43, an oligonucleotide probe specific to an “A” allele at nucleotide 101 of SEQ ID NO:44, an oligonucleotide probe specific to a “G” allele at nucleotide 307 of SEQ ID NO:45, and an oligonucleotide probe specific to an “A” allele at nucleotide 251 of SEQ ID NO:46, to form allele-specific hybridization complex(es) between the oligonucleotide probes and target alleles in the sample; assessing the binding between the oligonucleotide probes and the target alleles thereof, by detecting the allele-specific hybridization complexes; prognosing the human subject with UC with an earlier progression to colectomy based on the allele-specific hybridization complex(es) detected; and treating the human subject with UC prognosed with an earlier progression to colectomy by performing a surgical procedure comprising colectomy.