Patent ID: 9725757
Date: 2017-08-08
CPC Classifications: C12N,C12Q,G01N

Claim:
1. A method for detecting the presence or absence of a first target molecule in a sample, the method comprising: (a) contacting the sample with at least one molecular complex comprising a blocker oligonucleotide (BL) and a first catalytic nucleic acid enzyme, the BL comprising first and second segments hybridised to the first enzyme by complementary base pairing, and an intermediate segment between the first and second segments comprising a first substrate for a catalytic nucleic acid enzyme, wherein at least a segment of the first substrate is not hybridised to the first enzyme; (b) determining whether a detectable signal is generated by said contacting, wherein detection of the signal indicates the first target molecule is present, and failure to detect the signal indicates the first target molecule is absent.