Patent ID: 9567630
Date: 2017-02-14
CPC Classifications: C12M,C12Q,G01N

Claim:
1. A method for forming a lipid bilayer for use in a nanopore sensing device, comprising: (a) providing a primed chip comprising a fluid flow path in fluid communication with a plurality of sensing electrodes; (b) flowing a lipid solution into the fluid flow path; and (c) flowing at least one bubble onto the fluid flow path, thereby forming a lipid bilayer adjacent to the sensing electrodes, wherein the bubble spans the plurality of sensing electrodes, and wherein each of the sensing electrodes is in a well disposed on the trans side of the bilayer and opposite to the cis side of the bilayer wherein the top electrode is disposed; and wherein the primed chip does not comprise pre-deposited lipid molecules and/or pore proteins.