Patent ID: 9593381
Date: 2017-03-14
CPC Classifications: C12Q

Claim:
1. A method for simultaneously detecting a presence or an absence of a multitude of carbapenemase genes in a biological sample, said method comprising the steps of: a) carrying out an amplification reaction of a multitude of IMP genes, a multitude of KPC genes, and at least one of VIM, GES, and OXA-48 genes using: wherein the first set of primer pairs are for targeting multitude of IMP genes comprising at least variants IMP 1-24, the second set of primer pairs is for targeting multitude KPC genes comprising at least variants KPC1-7, and the third set of primer pairs is for targeting at least one of VIM, GES and OXA-48 genes; b) creating dissociation curves of the amplification products, said products being 100-300 bases long, and analyzing the dissociation curves, and c) determining the presence or absence of IMP and KPC genes, and at least one of VIM, GES, and OXA-48 genes, wherein the presence indicates that the biological sample comprises carbapenemase genes causing carbapenem resistance in bacteria.