Patent ID: 9549991
Date: 2017-01-24
CPC Classifications: A61K,C07K,C12P,G01N

Claim:
1. A method of generating monoclonal antibodies specific for an OP-Peptide within a compound of formula (I), wherein the OP-peptide is selected from the group consisting of H Linker is an amino acid residue or a residue from another bifunctional residue capable of covalently linking an OP-peptide to a CP of formula I; and CP is a carrier protein wherein the carrier protein is suitable for display of haptens for generation of antibodies selective and specific for an OP-peptide the method comprising: (a) immunizing an animal with a compound of formula (I); (b) fusing spleen cells from said immunized animal with myeloma cells to provide hybrid cells; (c) selecting hybrid cells producing antibodies selective and specific for the OP-peptide within the compound of formula (I) in comparison to a peptide-linker-CP compound corresponding in structure to formula (I) (d) obtaining purified cells by subcloning the selected hybrid cells from step c; and (e) isolating antibody produced by the purified cells of step (d) to obtain monoclonal antibody having sensitivity to at least 1:1000 dilution for the OP-peptide corresponding in structure to the OP-peptide within the compound of formula (I).