Patent ID: 9606345
Date: 2017-03-28
CPC Classifications: G01N,G02B

Claim:
1. A method of operating a microscope device comprising: providing a microscope device with an objective, a light source for illuminating a sample over an illumination beam path, an arrangement for producing a flat illumination pattern which is structured in two directions on the sample, a surface detector for detecting light coming from the sample over one picture beam path, an arrangement for shifting the illumination pattern on the sample in one displacement direction, and a control unit, the method further including taking one picture at a time of the light detected by the detector as a phase picture in different positions of the pattern along the displacement direction and computationally reconstructing from these phase pictures an overall Structured Illumination Microscopy (SIM) picture of the illuminated sample region, characterized in that the displacement direction is oblique to the main axes of symmetry of the illumination pattern and depending on the illumination pattern is chosen such that the number of phase pictures which is necessary for the reconstruction of the SIM picture corresponds to the theoretically minimally required value for the number of Fourier orders of the illumination pattern used in the computational reconstruction.