Patent ID: 9695431
Date: 2017-07-04
CPC Classifications: C07K,C12N

Claim:
1. A process for genetic transformation in Withania somnifera plants to increase secondary metabolite content in green house acclimatized plants, comprising: a) immersing a pre-cultured b) transferring the co-cultivated explant to a proliferation medium containing cefotaxime for 8-10 days so as to provide a tissue; c) determining Gus expression in the tissue by Gus assay followed by transferring Gus positive tissue to hygromycin B selection medium, thereby providing shoots; d) maintaining the shoots on hygromycin B while reducing the concentration of cefotaxime; and e) transferring the shoots to rooting medium to provide transformed plantlets and subjecting the transformed plantlets to greenhouse conditions; wherein the explant is an apical or nodal segment from a seedling shoot.