Patent ID: 9719133
Date: 2017-08-01
CPC Classifications: C12P,C12Q,G01N

Claim:
1. A kit for simultaneously qualitatively detecting and quantifying a microbial nucleic acid in a biological sample, the kit comprising a control nucleic acid which is an internal quantitative standard nucleic acid and a qualitative internal control nucleic acid, one or more primer pairs that hybridize to distinct sequence portions of the microbial nucleic acid and to distinct sequence portions of the control nucleic acid, at least a first probe labeled with a first donor fluorescent moiety and a first corresponding acceptor fluorescent moiety that hybridizes to the microbial nucleic acid, and at least a second probe labeled with a second donor fluorescent moiety and a second corresponding acceptor fluorescent moiety that hybridizes to the control nucleic acid, wherein the fluorescent moieties provide fluorescent signals for simultaneous qualitative detection and quantification of the microbial nucleic acid, and wherein the fluorescent signals generated by the control nucleic acid and the microbial nucleic acid are proportional to their concentration and are indicative of the qualitative detection and quantification of the microbial nucleic acid.