Patent ID: 9650599
Date: 2017-05-16
CPC Classifications: C12M

Claim:
1. A method for culturing cells using a culture medium, the method comprising, in order to replace the culture medium in a culture vessel: measuring a pH of the culture medium in the culture vessel to obtain a measured pH and filtering the measured pH at a cutoff frequency of 0.1 Hz to obtain a measured pH value value; replacing the culture medium when the measured pH value of the culture medium in the culture vessel is less than a predetermined pH threshold at a first replacement of the culture medium; and replacing the culture medium when the measured pH value of the culture medium in the culture vessel is less than the predetermined pH threshold and an interval is less than an interval of a previous replacement of the culture medium at second and subsequent replacements of the culture medium, wherein the predetermined pH threshold is within a range of greater than or equal to 6.8 and less than or equal to 7.0.