Patent ID: 9709558
Date: 2017-07-18
CPC Classifications: C40B,G01N

Claim:
1. A method of testing a vaccine, comprising (a) contacting antibodies in a blood sample of a subject immunized with a vaccine against a pathogenic microorganism with an array of different peptides of between 10-30 amino acids in length immobilized on the surface of the array, wherein each different peptide has a unique amino acid sequence, each of the different peptides occupying a distinct feature of the array, wherein each distinct feature of the array comprises peptides of a same sequence, wherein at least 50 of the different peptides have dissociation constants of between 1 mM and 1 μM to at least one antibody in the subject's sample, and wherein the array comprises at least 100,000 immobilized different peptides; (b) detecting a pattern of antibody binding of the blood sample to the immobilized different peptides in the array, wherein the pattern comprises binding interactions with dissociation constants of between 1 mM and 1 μM; and (c) comparing the binding interactions with dissociation constants of between 1 mM and 1 μM in the pattern of antibody binding of the blood sample of the subject to the pattern of antibody binding of one or more reference samples, wherein the reference samples are from subjects who have survived an infection with the pathogenic microorganism, wherein a plurality of peptides common to the pattern of antibody binding of the subject and pattern of antibody binding of the one or more reference samples indicates that the vaccine is effective against the pathogenic microorganism.