Patent ID: 9745563
Date: 2017-08-29
CPC Classifications: C12N,C12P,C12Y

Claim:
1. A mutant microbial host cell which has been modified to result in a deficiency in production of a polypeptide having α-amylase activity selected from the group consisting of: a. a polypeptide according to SEQ ID NO: 3 or 7 or a polypeptide at least 90% identical thereto; b. a mature polypeptide comprised in SEQ ID NO: 3 or 7 or a polypeptide at least 90% identical thereto; c. a polypeptide encoded by a polynucleotide according to SEQ ID NO: 1, 2, 5 or 6 or encoded by a polynucleotide at least 90% identical to SEQ ID NO: 1, 2, 5 or 6; d. a polypeptide encoded by a polynucleotide capable of hybridising to a polynucleotide according to SEQ ID NO: 1, 2, 5 or 6 or capable of hybridising to the complementary strand of a polynucleotide according to SEQ ID NO: 1, 2, 5 or 6; wherein the modification comprises a modification which results in a reduced or no production of a polypeptide having α-amylase activity as defined in a)-d) compared to the parent microbial host cell that has not been modified; wherein the modification is made in the genome of the microbial host cell; wherein the modification is selected from the group consisting of: 1) a full or partial deletion of a polynucleotide as defined in c) or d); 2) a full or partial replacement of a polynucleotide as defined in c) or d) with a polynucleotide sequence which does not code for a polypeptide as defined in a)-d) or which code for a partially or fully inactive form of a polypeptide as defined in a)-d); 3) a disruption of a polynucleotide as defined in c) or d) by the insertion of one or more nucleotides in the polynucleotide sequence and consequent partial or full inactivation of the polypeptide as defined in a)-d); wherein the mutant microbial host cell is an