Patent ID: 9574180
Date: 2017-02-21
CPC Classifications: A61K,A61P,C12N

Claim:
1. A method of obtaining beta-islet cells from pancreases of rabbits, the method comprising: harvesting pancreases of newborn rabbits and placing the pancreases in a salt solution comprising an antibiotic at a temperature of 4-10° C.; removing vessels and excretory ducts from the harvested pancreases; obtaining minced pancreatic micro fragments from said pancreases; incubating said minced pancreatic micro fragments in a serum free medium at a first incubation temperature 36.6° C. to 37° C. for 6 to 10 days at 0% to 5% CO2; for a first incubation period and periodically replacing the serum free medium and removing spontaneously destroyed unwanted cells comprising exocrine cells and blood cells and elements of connective tissue until at least 80% of remaining cells are beta-islet cells; and incubating said minced pancreatic micro fragments in said serum free medium at a second incubation temperature 22° C. to 29° C. for 4 to 5 days for a second incubation period until 78-90% of remaining cells are beta-islet cells, wherein said serum free medium is optionally periodically replaced, and thereby obtaining islet cell clusters wherein 78-90% are beta-islet cells.