Patent ID: 9580762
Date: 2017-02-28
CPC Classifications: C12Q

Claim:
1. A method of establishing whether a biological sample contains WNV (West Nile virus), the method comprising the steps of: (a) obtaining nucleic acids from the biological sample; (b) performing an in vitro nucleic acid amplification reaction comprising nucleic acids obtained in step (a) as templates, and further comprising a hybridization probe labeled with one or more detectable labels selected from the group consisting of: a chemiluminescent compound; a fluorophore moiety; and a quencher moiety, wherein said hybridization probe is up to 60 nucleotides in length and comprises a base sequence selected from the group consisting of SEQ ID NO:104, SEQ ID NO:105, SEQ ID NO:106, SEQ ID NO:107, SEQ ID NO:108, SEQ ID NO:109, SEQ ID NO:110, SEQ ID NO:111, SEQ ID NO:112, SEQ ID NO:113, SEQ ID NO:114, SEQ ID NO:115, and SEQ ID NO:116, whereby there is synthesized, if nucleic acids obtained in step (a) comprise WNV nucleic acids, an amplification product terminating at one end with the nucleotide sequence of SEQ ID NO:74 or the complement thereof, allowing for RNA and DNA equivalent bases, and wherein there is formed by complementary base pairing, if nucleic acids obtained in step (a) comprise WNV nucleic acids, a nucleic acid duplex comprising the hybridization probe and a strand of the amplification product synthesized in step (b); (c) determining whether the nucleic acid duplex formed in the in vitro nucleic acid amplification reaction; and (d) establishing either that,