Patent ID: 9658211
Date: 2017-05-23
CPC Classifications: C12M,G01N

Claim:
1. A method of mimicking a pathological or physiologic condition in vitro, the method comprising: adding a culture media and at least one factor to a cell culture container; plating a first cell type on a first surface of a porous membrane, and optionally plating a second cell type on a second surface of the porous membrane, wherein the porous membrane is suspended in the cell culture container such that the first surface is proximal and in spaced relation to a bottom surface of the cell culture container, thereby defining within the cell culture container a lower volume comprising the first cell type and an upper volume comprising the optional second cell type, the porous membrane being adapted to permit fluid communication of the cell culture media and physical interaction and communication between cells of the first cell type and cells of the optional second cell type; and applying a shear force upon the second cell type or the second surface of the porous membrane in the upper volume, the shear force resulting from flow of the culture media induced by a flow device, the flow mimicking flow to which the at least one cell type is exposed in vivo in the pathological or physiologic condition and the flow device comprising a body adapted for being positioned in the culture media in the upper volume of the container and a motor adapted to rotate the body, wherein: for mimicking the pathological condition, the factor is exogenously added to the cell culture container to provide a concentration that is either: for mimicking the physiologic condition, the factor is exogenously added to the cell culture container to provide a concentration that is either: