Patent ID: 9568454
Date: 2017-02-14
CPC Classifications: B01D,G01N,H01J,Y10T

Claim:
1. A method of separating lipids of different classes from a biological sample, said method comprising steps of i) preparing the biological sample; ii) loading said biological sample onto an ultrahigh performance liquid chromatography (UHPLC) system, wherein said UHPLC system comprises a hydrophilic interaction chromatography (HILIC) column; iii) eluting said UHPLC column with an elution solvent, wherein the elution solvent is obtained by mixing a mobile phase A and a mobile phase B in situ, and wherein a volume ratio of the mobile phase B to the mobile phase A is at a gradient that increases during the elution step; and iv) detecting lipids of different classes by using a mass spectrometer.