Patent ID: 9644183
Date: 2017-05-09
CPC Classifications: A61K,A61P,C12N

Claim:
1. A method of differentiating adipose stem cells into vascular endothelial cell clusters for the induction of angiogenesis comprising: applying adipose stem cells to a culture plate having a hydrophobic surface and a growth factor immobilized thereon at a concentration of 1×10 allowing the stem cells to adhere to the surface of the plate by physical attraction of the stem cells to the hydrophobic surface and by biological attraction of the stem cells to the growth factor; culturing the stem cells in a culture medium while being attached to the surface of the culture plate, and subsequently forming a three-dimensional cell cluster detached from the culture plate and floating in the culture medium, wherein the culture medium is a mixture of DMEM, Ham's F12, and serum; and culturing the three-dimensional cell cluster in the culture medium to induce differentiation of the stem cells into vascular endothelial cells.