Patent ID: 9603881
Date: 2017-03-28
CPC Classifications: A61K,A61P,A61Q,C12N

Claim:
1. A method for obtaining a bioactive fraction from a photosynthetic organism, said method comprising: providing an aquatic photosynthetic organism selected from the group consisting of cultivating the aquatic photosynthetic organism under stress-inducing cultivation conditions comprising subjecting the aquatic photosynthetic organism to a stress factor or to a plurality of stress factors; separating the stress-induced aquatic photosynthetic organism into cell juice and a cell walls component; treating the cell juice under conditions effective to yield a bioactive fraction, wherein said bioactive fraction is selected from the group consisting of a cell serum fraction, a membrane fraction, a cell juice supernatant fraction, and a cell serum filtrate fraction; and isolating said bioactive fraction from the treated cell juice, wherein the stress factor or plurality of stress factors are selected from the group consisting of ultraviolet light stress by applying UVB irradiance of 1-2 mW/cm wherein the isolated bioactive fraction has at least one altered characteristic compared to a corresponding bioactive fraction isolated from a non-stress-induced aquatic photosynthetic organism, and said characteristic is selected from the group consisting of physico-chemical properties, surface modification properties, moisturization properties, anti-inflammatory activity, and anti-ageing activity, wherein the physico-chemical properties are properties selected from the group consisting of surface tension, dry matter content, and osmolality, and wherein the anti-inflammatory and/or anti-ageing activities are selected from the group consisting of elastase inhibition, trypsin inhibition, anti-oxidant activity, and free-radical scavenging activity.