Patent ID: 9714897
Date: 2017-07-25
CPC Classifications: C12Q,G01N

Claim:
1. A method for determining if a sample includes one or more of lymphocytes and monocytes, comprising: inserting, into a cytometric device, a plurality of hydrogel particles suspended in an aqueous solution, the plurality of hydrogel particles including: (1) a first portion of hydrogel particles having forward scatter, side scatter, and hydrodynamic properties substantially similar to a lymphocyte, and (2) a second portion of hydrogel particles having forward scatter, side scatter, and hydrodynamic properties substantially similar to a monocyte, wherein each hydrogel particle of the plurality of hydrogel particles comprises a polymerized monomer and has at least one functionalized surface having at least one cell surface marker; measuring the forward scatter, side scatter, and hydrodynamic properties of each hydrogel particle of the plurality of hydrogel particles using the cytometric device; calibrating the cytometric device based on the measured forward scatter, side scatter, and hydrodynamic properties of the plurality of hydrogel particles; adding a sample comprising a plurality of cells to the calibrated cytometric device; and measuring the forward scatter, side scatter, and hydrodynamic properties of each cell of the plurality of cells using the calibrated cytometric device to determine if the sample includes one or more of lymphocytes and monocytes.