Patent ID: 9624538
Date: 2017-04-18
CPC Classifications: C12Q

Claim:
1. A method of sequencing a polynucleotide immobilized on an array comprising: synthesizing a concatemer of a polynucleotide by rolling circle amplification; immobilizing the concatemer to the array, wherein the array comprises a substrate having a plurality of specific functionalized regions attached to the substrate in a pattern forming an ordered array, wherein each specific functionalized region comprises an inner spot functionalized with a first group which serves to capture the concatemer and an outer spot functionalized with a second group which serves as an attachment point for an amplification primer, wherein the concatermer is immobilized to the inner spot, and wherein the concatemer has a cross-sectional diameter greater than the diameter of the inner spot; and sequencing the immobilized concatemer.