Patent ID: 9726679
Date: 2017-08-08
CPC Classifications: G01N

Claim:
1. A spectral method for quantifying hemoglobin fragility caused by smoking, comprising the steps of: obtaining control blood samples from at least one non-smoking patient and from a patient having a history of long-term smoking; separating blood plasma from the blood samples; obtaining synchronous excitation spectra (SXS) of the blood plasma samples with a spectrofluorometer at a scan offset of 70 nm; comparing the long-term smoking patient's SXS spectrum with the control SXS spectrum; diagnosing hemoglobin fragility when the excitation maximum for tryptophan at 290 nm of the long-term smoking patient is 60% of the control sample; obtaining synchronous excitation spectra (SXS) of the blood plasma samples with a spectrofluorometer at a scan offset of 10 nm; comparing the long-term smoking patient's SXS spectrum with the control SXS spectrum; diagnosing hemoglobin fragility when the excitation maxima of NADH, FAD, and porphyrin for the long-term smoking patient are 30% to 70% higher than the maxima for these metabolites in the control sample; computing the ratios R diagnosing hemoglobin fragility when R