Patent ID: 9708674
Date: 2017-07-18
CPC Classifications: A01H,C12Q

Claim:
1. A method of producing a soybean plant tolerant to iron deficiency chlorosis (IDC), comprising the steps of; (a) isolating a nucleic acid from a soybean plant or part thereof; (b) detecting in said nucleic acid, the presence of a plurality of single nucleotide polymorphism (SNP) markers associated with IDC tolerance in a soybean plant, wherein said markers are located within a chromosomal interval comprising physical positions 1035989-1401213 on Soybean chromosome 5, and further wherein said chromosomal interval comprises (i) a G allele at SY0152AQ; (ii) a G allele at SY0723BQ; (iii) a G allele at SY0724AQ; (iv) a SEQ ID NO: 301 insertion at SY1154AQ; and (v) an A allele at SY0153AQ as described in Table 2; (c) identifying and/or selecting an iron deficiency chlorosis (IDC) tolerant soybean plant or part thereof; (d) crossing the soybean plant of (c) with a second soybean plant not having said plurality of markers of step (b) in its genome; (e) collecting seed from the cross in step (d); and (f) growing a progeny soybean plant from said seed which comprises said plurality of markers in its genome, thereby producing a soybean plant with increased tolerance to IDC relative to a soybean plant not comprising said plurality of markers; wherein said plurality of markers identifies a soybean plant comprising all of SNP alleles (i)-(v).