Patent ID: 9551040
Date: 2017-01-24
CPC Classifications: C12Q,Y02A

Claim:
1. A method for determining the presence of Leishmania species selected from the group consisting of: Leishmania tropica, Leishmania chagasi, Leishmania infantum, Leishmania donovani, Leishmania major, Leishmania braziliensis, Leishmania guyanensis, Leishmania panamensis, Leishmania mexicana , and Leishmania amazonensis in a sample, and distinguishing the Leishmania species so determined from other Leishmania species present in the sample, the method comprising the following steps: (a) forming (b) producing pyrophosphates and double-stranded DNAs containing the primer sequences by exposing the (c) producing additional pyrophosphates and double-stranded DNAs containing the primer sequences by performing temperature cycles; (d) determining the melting temperature of the double-stranded DNA produced; (e) comparing the determined melting temperature to a melting temperature that is characteristic of a particular (f) determining whether the particular (g) determining which of the wherein steps (a) through (f) are repeated using at least one nucleic acid primer pair that is different for each Leishmania species in the group.