Patent ID: 9658197
Date: 2017-05-23
CPC Classifications: G01N,Y10T

Claim:
1. A method for judging an abnormality of a particle analyzer which comprises a measuring sample preparation unit configured to prepare a measuring sample by mixing a urine sample with a fluorescence dye, a light source configured to irradiate the measuring sample with a light and a fluorescence detector configured to detect fluorescence from the measuring sample, comprising steps of: providing a polyacrylamide particle which is fluorescently unstained, a hydrophilic vinyl polymer particle which is fluorescently unstained, and a fluorescent latex particle; contacting, with the measuring sample preparation unit, a first fluorescence dye with the polyacrylamide particle and the hydrophilic vinyl polymer particle to fluorescently stain the polyacrylamide particle and the hydrophilic vinyl polymer particle, the polyacrylamide particle when stained fluorescently being similar in fluorescence intensity to an epithelial cell in the urine sample which is fluorescently stained with the first fluorescence dye, and the hydrophilic vinyl polymer particle when stained fluorescently being similar in fluorescence intensity to a columnar cell in the urine sample which is fluorescently stained with the first fluorescence dye, contacting, with the measuring sample preparation unit, a second fluorescence dye with the fluorescent latex particle, the fluorescent latex particle being substantially not able to be stained by the first and second fluorescence dyes, and the fluorescent latex particle being similar in fluorescence intensity to bacteria which are stained by the measuring sample preparation unit; irradiating with the light source a light to the polyacrylamide particle, the hydrophilic vinyl polymer particle and the fluorescent latex particle; detecting with the fluorescence detector a first fluorescence from polyacrylamide particle, a second fluorescence from the hydrophilic vinyl polymer particle, and a third fluorescence from the fluorescent latex particle; comparing the first fluorescence with a condition corresponding to expected fluorescence of the polyacrylamide particle stained fluorescently with the first fluorescence dye defined by predetermined upper and lower limit values; comparing the second fluorescence with a condition corresponding to expected fluorescence of the hydrophilic vinyl polymer particle stained fluorescently with the first fluorescence dye defined by predetermined upper and lower limit values; comparing the third fluorescence with a condition corresponding to expected fluorescence of the fluorescent latex particle defined by predetermined upper and lower limit values; and judging an abnormality of the particle analyzer when at least one fluorescence selected from the group consisting of the first, second and third fluorescence is outside the predetermined upper and lower limit values.