Patent ID: 9588110
Date: 2017-03-07
CPC Classifications: C07K,C12N,C12Q,C12Y,G01N

Claim:
1. A method for detecting the concurrent presence of at least two targets within a biological sample, comprising: (a) contacting said biological sample with a first binding agent and a second binding agent, wherein said first binding agent is operably linked to a first sortase and specifically binds to a first target, and wherein said second binding agent is operably linked to a first sortase recognition sequence and specifically binds to a second target; (b) adding a sortase substrate under conditions where a first sortase-mediated ligation of the sortase substrate to the first sortase recognition sequence will produce a ligation product, and (c) detecting the ligation product, wherein detection of said ligation product indicates the concurrent presence of the first target and the second target in the biological sample.