Patent ID: 9617296
Date: 2017-04-11
CPC Classifications: C07H,C07K,C12Q,G01N

Claim:
1. A method for breaking a water-in-oil emulsion including one or more biomolecules dispersed in an aqueous phase of the water-in-oil emulsion, comprising: a) obtaining a first emulsion including a continuous hydrophobic fraction and a discontinuous aqueous fraction, the aqueous fraction having one or more biomolecules dispersed therein; b) breaking the first emulsion by contacting the first emulsion with 1-octanol to form a first mixture; c) centrifuging the first mixture to form a top phase, an interface and a bottom phase; d) removing and the top phase of step (c) and retaining the interface and bottom phase; e) contacting the interface and bottom phase with N-butanol to form a second mixture; f) centrifuging the second mixture to form a top phase, an interface and a bottom phase; and g) removing the top phase of step (f) and retaining the interface and bottom phase.