Patent ID: 9676846
Date: 2017-06-13
CPC Classifications: A61K,C07K,G01N

Claim:
1. An isolated nucleic acid molecule encoding a single chain Fv protein (scFv), wherein the scFv comprises a heavy chain variable region and a light chain variable region, wherein the heavy chain variable region comprises the amino acid sequence set forth as amino acids 26-33 of SEQ ID NO: 7, amino acids 51-58 of SEQ ID NO: 7, and amino acids 97-109 of SEQ ID NO: 7, wherein residue 56 is G and residue 109 is N; and wherein the light chain variable region comprises the amino acid sequence set forth as amino acids 27-32 of SEQ ID NO: 8, amino acids 50-52 of SEQ ID NO: 8 and amino acids 89-97 of SEQ ID NO: 8, and wherein the scFv specifically binds insulin-like growth factor II (IGF-II) with an equilibrium dissociation constant (K