Patent ID: 9631017
Date: 2017-04-25
CPC Classifications: C07K,C40B

Claim:
1. A method for making a Fab library, comprising: providing a plurality of vectors, wherein each of the plurality of vectors comprises (a) a first cloning region, and a second cloning region, wherein each cloning region comprises at least one, for the vector unique, restriction enzyme cleavage site, and each cloning region is 5′ flanked by a ribosome binding site and a signal sequence (b) a polynucleotide encoding an anchor region located 3′ of the second cloning region, and (c) a polynucleotide encoding a heavy chain constant region or a portion thereof located between the vector unique restriction enzyme cleavage site of the second cloning region and the anchor region; introducing into the first cloning region a member of a first plurality of variable polynucleotides encoding a first plurality of variable polypeptides, each of which comprises an antibody light chain variable region, wherein each of the first plurality of variable polypeptides further comprises a light chain constant region fused to the C-terminus of the light chain variable region, separately introducing into the second cloning region of each vector a member of a second plurality of variable polynucleotides encoding a second plurality of variable polypeptides, each of which comprises an antibody heavy chain variable region, thereby producing a plurality of vectors encoding the first and second plurality of variable polypeptides, and introducing the plurality of vectors encoding the first and second plurality of variable polypeptides into host cells to provide a plurality of capsid particles, each comprising a member of the first plurality of variable polynucleotides and a member of the second plurality of variable polynucleotides, wherein the polypeptides encoded by the members of the first and second plurality of variable polynucleotides form a Fab molecule; wherein the first and second plurality of variable polypeptides form a plurality of Fab molecules.