Patent ID: 9573982
Date: 2017-02-21
CPC Classifications: C07K,G01N

Claim:
1. A modified biotin-binding protein comprising any one of following (a) and (b): (a) an amino acid sequence having 1 to 7 amino acid mutations in the amino acid sequence of SEQ ID NO: 2 and having biotin-binding activity, and (b) an amino acid sequence having an identity of not less than 95% to the amino acid sequence of SEQ ID NO: 2 and having biotin-binding activity; wherein an asparagine residue at position 115 of SEQ ID NO: 2 is replaced with cysteine, wherein the modified biotin-binding protein maintains biotin-binding activity of not less than 75% after a heat treatment at 99.9° C. for 30 min, compared with the activity before the treatment, and wherein the modified biotin-binding protein maintains biotin-binding activity of not less than 50% after a treatment in 60% aprotic polar organic solvent for 30 min, compared with the activity before the treatment.