Patent ID: 9617335
Date: 2017-04-11
CPC Classifications: C07K,C12N,C12P,C12Y

Claim:
1. An Escherichia coli host cell comprising two or more types of expression constructs, wherein the expression construct of each type comprises an inducible promoter and a polynucleotide sequence encoding a gene product to be transcribed from the inducible promoter; and at least one of said inducible promoters is responsive to an inducer that is different than the inducer of another of said inducible promoters; and each inducible promoter is selected from the group consisting of: an L-arabinose-inducible promoter, a propionate-inducible promoter, a rhamnose-inducible promoter, and a D-xylose-inducible promoter; and wherein the host cell has a reduced level of gene function of at least one gene encoding a protein that metabolizes an inducer of at least one said inducible promoter; and wherein the host cell has a reduced level of gene function of a gene that encodes a reductase; and at least one of said gene products forms a multimer with another of said gene products; and at least one of said gene products is a polypeptide that lacks a signal peptide.