Patent ID: 9587277
Date: 2017-03-07
CPC Classifications: C12Q,G01N,Y10T

Claim:
1. A method for sequencing a nucleic acid template, the method comprising: forming a set of nanowire assemblies each including a separate semiconductor nanowire and a different probe covalently bound to the semiconductor nanowire, the semiconductor nanowire connected at a first end to a first electrode and at a second end to a second electrode, a detector connected to the electrodes to detect an electrical characteristic of the semiconductor nanowire; contacting the set of nanowire assemblies with a template nucleic acid, the template nucleic acid forming nucleic acid duplexes with a plurality of the different probes; contacting the nucleic acid duplexes with an extension nucleic acid, the extension nucleic acid joined to a probe of the plurality of different probes; disrupting the nucleic acid duplexes to form single strand nucleic acid; and measuring an electrical characteristic of a nanowire assembly of the set of nanowire assemblies.