Patent ID: 9658215
Date: 2017-05-23
CPC Classifications: G01N

Claim:
1. A method for enumerating early granulocyte cells (EGCs) in a blood sample, comprising: analyzing unmodified white blood cells of the blood sample using a first calculated parameter measured by a non-fluorescent instrument resulting in analysis data, wherein the first calculated parameter includes a low angle light scatter (LALS) measurement and an additional parameter, wherein the additional parameter is a direct current (DC) measurement or a medium angle light scatter (MALS) measurement; separating the EGCs from other white blood cells based upon the first calculated parameter and further based upon a second calculated parameter, wherein the second calculated parameter includes an opacity (OP) measurement, wherein the OP measurement is calculated from DC and radiofreguency (RF) measurements; and enumerating the separated EGCs, wherein the method is a non-fluorescent method.