Patent ID: 9637522
Date: 2017-05-02
CPC Classifications: A61K,A61P,C07K,C12N

Claim:
1. A fusion protein, comprising an influenza hemagglutinin (HA) stem antigen where HA globular head domain, residues 60-291 in H3 numbering is replaced with a linker peptide, signal peptide residues 2-17, transmembrane domain residues 521-554 and cytoplasmic tail residues 555-566 are deleted, where amino acid numbering is made relative to SEQ ID NO:1, wherein the fusion protein comprises not more than two disulfide bonds, and which HA stem domain is modified by (a) substitution of at least one hydrophobic amino acid with at least one polar amino acid, (b) deletion of regions in the stem containing hydrophobic residues and cysteines; (c) fusion to a foldon trimerization domain.