Patent ID: 9631057
Date: 2017-04-25
CPC Classifications: C08H,C12P,C13K,G01N

Claim:
1. A method for producing glucose for fermentation said method comprising: (a) measuring the initial hydrolysis rate of a biomass; (b) correlating the measured initial hydrolysis rate with a crystallinity index of standard cellulosic materials to project a crystallinity indicative of overall enzymatic hydrolysis susceptibility wherein the crystallinity index was determined by applying multivariate statistical analysis to X-ray diffraction spectra, solid state cross-polarization/magic angle spinning nuclear magnetic resonance spectra, infrared spectra, differential scanning calorimetry heat capacity temperature plots, or a combination thereof; (c) selecting one or more of the reaction conditions of steps (d)-(f) based on the projected crystallinity of the biomass; (d) treating a biomass with acid and heat to produce a composition mixture comprising cellulose suitable for enzymatic hydrolysis; (e) enzymatically hydrolyzing at least a portion of the cellulose of step (d) to form a composition comprising glucose; and (f) utilizing glucose in an aerobic or anaerobic fermentation process.