Patent ID: 9678077
Date: 2017-06-13
CPC Classifications: C07K,G01N

Claim:
1. A triple immunohistochemistry (IHC) assay for in situ detection of TFF3, ERG, and a high-molecular weight cytokeratin (HMWCK), comprising: providing a prostate tissue sample comprising fixed cells; contacting the sample with a first primary antibody directed to a first molecule selected from ERG, TFF3 or HMWCK, a second primary antibody directed to a second molecule selected from ERG, TFF3 or HMWCK, and a third primary antibody directed to a third molecule selected from ERG, TFF3 or HMWCK, to permit formation of antibody-antigen complexes in the sample which lead to the generation of detectable signals; and identifying cells that display a detectable signal.