Patent ID: 9631226
Date: 2017-04-25
CPC Classifications: C07K,C12Q,G01N

Claim:
1. A method of validating a chromatin immunoprecipitation (ChIP) assay result, the method comprising: (a) obtaining an input sample of the genomic material of interest and of one or more polypeptide-oligonucleotide conjugate of the formula: wherein A is a polypeptide comprising 5 amino acids, L is a linker and N is an oligonucleotide comprising 10 nucleotides, wherein the sequence of the 10 nucleotides of N uniquely identifies an amino acid sequence and/or amino acid modification of A wherein A comprises an amino acid sequence derived from a histone protein selected from the group consisting of histone HI, H2A, H2AX, H2B, H3 and H4, (b) performing a ChIP assay, thereby processing, in parallel to the genomic material of interest of (a), the one or more polypeptide-oligonucleotide conjugates of (a), (c) obtaining a processed sample of the polypeptide-oligonucleotide conjugate and the genomic material of interest processed in (b), and (d) analyzing the samples obtained in (c), thereby obtaining a value and/or signal for each of the analyzed processed samples, and (e) validating the ChIP assay result based on the value and/or signal obtained in (d).