Patent ID: 9580734
Date: 2017-02-28
CPC Classifications: A61K,A61P,C07K,C08B,C12N,C12P

Claim:
1. A method for preparing a capsular polysaccharide of Neisseria meningitidis X with high yield and high purity, comprising the steps of: (a) preparing an aqueous fermenter nutrient medium comprising casamino acid; (b) inoculating the fermenter nutrient medium with a (c) continuous exponential feeding with a feed solution with a feed addition rate varying between 10 mL/hr/1.5 L and 60 mL/hr/1.5 L beginning at an OD at 590 nm between 3 and 4; (d) incubating the inoculated fermenter nutrient medium under controlled conditions of pH, temperature and dissolved oxygen percentage; (e) harvesting the capsular polysaccharide produced in step (d) when the optical density (OD) at 590 nm is less than 60% of the highest culture OD at 590 nm; (f) purifying the capsular saccharide obtained in step (e); (g) optionally concentrating the purified capsular polysaccharide obtained in step (f); and wherein said purified polysaccharide has yield from 300 to 550 mg/L, average molecular weight from 400 to 550 KDa, purification step recovery from 60% to 65% and contains less than 0.5% proteins/peptides, less than 0.5% nucleic acids and less than 5 EU/μg endotoxins.