Patent ID: 9720003
Date: 2017-08-01
CPC Classifications: G01N

Claim:
1. A method for measuring Troponin I in a sample with 1 pM limit of detection, comprising at least the following steps: a. providing a sample comprising Troponin I to be measured; b. contacting the sample with three monoclonal anti-Troponin I antibodies coupled to a labeled polymer coated iron oxide particle having a size of from 200 to 1000 nm; c. applying a first magnetic field in the direction of a sensor surface, thereby contacting the particles with a polyclonal anti-Troponin I antibody coupled to the sensor surface, thereby causing at least some of the particles to be bound to the sensor surface, wherein the polyclonal anti-Troponin I antibody binds to Troponin I at the same time as the monoclonal anti-Troponin I antibody; d. applying pulsed actuation by discontinuing the first magnetic field, thereby allowing unbound particles to move over the sensor surface, reapplying the first magnetic field, and discontinuing the first magnetic field, thereby increasing the number of particles bound to the sensor surface, and alternating the application of pulsed actuation with the application of magnetic pulses causing translational and rotational movement of the particles over the sensor surface, wherein the pulsed actuation and magnetic pulses are applied for less than five minutes; and e. applying a second magnetic field in a direction away from the sensor surface to remove unbound particles, and thereafter detecting the labeled polymer coated iron oxide particles on the sensor surface; wherein the three monoclonal anti-Troponin I antibodies are clones A34500, 267, and 560.