Patent ID: 9605270
Date: 2017-03-28
CPC Classifications: C07K,C12N

Claim:
1. A plant expression system for the expression of a heterologous gene in a plant or plant cell culture, the plant expression system comprising a cDNA encoding a CMV1a replicase and a cDNA encoding a CMV2a replicase, wherein the cDNA encoding the CMV1a replicase comprises a CMV RNA 1 cDNA with a 5′ nucleotide sequence deletion consisting of deletion of 57 nucleotides from the 5′ end of the CMV RNA 1 cDNA, wherein said nucleotide sequence deletion prevents replication of CMV1a transcripts when a functional replicase is present, and wherein the cDNA encoding the CMV1a replicase is operably linked to a LEX operator and expression of the CMV1a replicase is estradiol-inducible.