Patent ID: 9597372
Date: 2017-03-21
CPC Classifications: A61K,A61P,C07H,C07K,C12N,C12Y,G01N

Claim:
1. A method of preparing a composition comprising a vault complex, said vault complex comprising a vault particle and a cytokine fusion protein, the method comprising a) mixing the cytokine fusion protein with the vault particle to generate a mixture, wherein the cytokine fusion protein comprises a cytokine and a vault targeting domain, which said vault targeting domain is a vault poly ADP-ribose polymerase (VPARP) sequence or a portion thereof that interacts with the major vault protein, or a telomerase/vault associated protein 1 (TEP 1) sequence or a portion thereof that interacts with the major vault protein, and wherein the vault particle comprises a major vault protein (MVP); and b) incubating the mixture for a sufficient period of time to allow non-covalent association of the cytokine fusion protein with the vault particle, thereby generating the vault complex.