Patent ID: 9683983
Date: 2017-06-20
CPC Classifications: B01L,B03D,B04B,C12M,G01N

Claim:
1. An insert for a centrifuge tube, the insert comprising: a member sized to fit within the tube for dividing the tube into a top portion and a bottom portion, a support extending or depending from the member for positioning the member within the tube, wherein the support limits a distance between the member and a bottom of the tube when the support is pushed towards a bottom end of the centrifuge tube, and at least two openings through the member so that a first opening is closer to the bottom end of the tube relative to a second opening when the insert is positioned in the centrifuge tube, wherein the first opening allows fluid communication between a space in the top portion of the tube above the member and a space in the bottom portion of the tube below the member when the insert is positioned in the centrifuge tube, and wherein the second opening allows air to escape from the space below the member when the bottom portion of the tube is filled with a liquid density separation media (DSM) through the first opening and the at least two openings are sized to provide surface tension across the openings to prevent DSM contained below the member to flow through the openings when the tube is inverted.