Patent ID: 9636354
Date: 2017-05-02
CPC Classifications: A61K,C12N

Claim:
1. A method for expressing a heterologous nucleic acid segment of interest in a mammalian host cell, comprising providing to a population of mammalian cells, an expression vector comprising, in 5′ to 3′ order, (a) a first isolated HSV LAT insulator/boundary region that consists essentially of a contiguous nucleotide sequence from about nucleotide 8365 to about nucleotide 9273 of SEQ ID NO:102, SEQ ID NO:103, or SEQ ID NO:104, operably positioned 5′ of a first polynucleotide comprising the heterologous nucleic acid segment of interest operably linked to a first promoter that expresses the heterologous nucleic acid segment in one or more cells of the population; and (b) a second isolated HSV LAT insulator/boundary region that consists essentially of a contiguous nucleotide sequence from about nucleotide 120,208 to about nucleotide 120,940 of SEQ ID NO:102, SEQ ID NO:103, or SEQ ID NO:104, operably positioned 3′ of the heterologous nucleic acid segment of interest; in an amount and for a time effective to express the heterologous nucleic acid segment in one or more cells of the population.