Patent ID: 9746470
Date: 2017-08-29
CPC Classifications: C12Q,G01N

Claim:
1. A method for detecting a protein-protein interaction, the method comprising steps of: (a) providing a lysate prepared from cells that express a prey protein and a bait protein, wherein the prey protein is labeled with a detectable label and the bait protein is tagged with a heterologous epitope tag that allows separation of the bait protein and protein(s) interacting with the bait protein from other proteins in the lysate; (b) immobilizing the tag to one or more inner surface(s) of a well, thereby immobilizing the bait protein and protein(s) interacting with the bait protein; (c) detecting immobilized prey protein by detecting the detectable label, thereby detecting a protein-protein interaction; and (d) detecting immobilized bait protein and measuring the protein-protein interaction based on the ratio of prey protein to bait protein detected.