Patent ID: 9623083
Date: 2017-04-18
CPC Classifications: A61K,A61P,C12N,C12Q,G01N

Claim:
1. A method to screen for an activator of adipose-resident NKT cells which comprises (a) feeding at least one mouse a high fat diet (HFD) for at least about 4 days up to about 24 weeks; (b) administering a candidate activator to said at least one mouse; (c) sacrificing said at least one mouse from about 4 to about 7 days after administering said activator; (d) preparing white adipose tissue (WAT) from said at least one mouse; and (e) detecting whether (i) said WAT contains increased expression, relative to expression in WAT from a control mouse of any one or more of IL-4, IL-13, IL-12, Arginase 1 (Arg1), chitinase 3-like 3 (Chi313), C-type lectin domain family 7, member a (Clec7a), Programmed cell death 1 ligand 2 (Pdc11g2), Resistin like alpha (Retn1a), Interleukin 1 receptor antagonist (IL-1rn), and Interleukin receptor 4 alpha (IL-4ra), (ii) M2 macrophage polarization increases in said WAT relative to that in WAT from said control mouse, (iii) whether at least one T