Patent ID: 9663792
Date: 2017-05-30
CPC Classifications: C12N

Claim:
1. A method of transforming a plant cell, the method comprising: 1) introducing into the plant cell a first nucleic acid sequence that comprises a plant nuclear promoter operably linked to a first nucleic acid sequence that comprises a plant mitochondrion transgene cassette, a plant mitochondrion translocation sequence from a group II intron RNA, wherein the group II intron is Ll.LtrB, and a primer binding domain; and 2) introducing into the plant cell a second nucleic acid sequence that encodes for a translocation sequence binding protein from an intron encoded protein (IEP) of a group II intron, wherein the IEP is LtrA fused to a plant mitochondrion transit peptide, wherein the second nucleic acid sequence is operably linked to a plant nuclear promoter, and wherein the mitochondrion translocation sequence of the product of 1) binds with the translocation sequence binding protein of the product of 2).