Patent ID: 9689037
Date: 2017-06-27
CPC Classifications: C12Q

Claim:
1. A kit for assessing an organism for a genetic or epigenetic element, wherein said kit comprises: (a) a probe nucleic acid coupled to an amplifying restriction endonuclease and comprising a nucleotide sequence complementary to a sequence of a target nucleic acid containing said genetic or epigenetic element, wherein at least a portion of said target nucleic acid is capable of hybridizing to at least a portion of said probe nucleic acid to form a double-stranded portion of nucleic acid comprising a restriction endonuclease cut site, and (b) a signal expansion nucleic acid (i) coupled to a label and an amplifying restriction endonuclease and (ii) comprising a double-stranded portion of nucleic acid comprising said amplifying restriction endonuclease cut site of said probe nucleic acid.