Patent ID: 9715573
Date: 2017-07-25
CPC Classifications: C12Q,G16B

Claim:
1. A method for nucleic acid sequence data assembly, comprising: (a) obtaining purified DNA; (b) binding the purified DNA with a DNA binding agent to form DNA/chromatin complexes; (c) incubating the DNA-chromatin complexes with restriction enzymes to leave sticky ends; (d) performing ligation to join ends of DNA; (e) sequencing ligated DNA junctions to generate paired end reads; (f) obtaining standard paired-end read distance frequency data; (g) obtaining grouped contig sequences; and (h) scaffolding the grouped contig sequences such that read pair distance frequency data for read pairs that map to separate contigs approximates the standard paired-end read distance frequency data, thereby assembling the sequence data of the nucleic acid.