Patent ID: 9568839
Date: 2017-02-14
CPC Classifications: B01J,B01L,B82Y,C12N,C12P,C40B,G03F,G16B

Claim:
1. A method for optimizing the functional genetic parameters of genetic elements, comprising: (a) providing a digital sequence of a variant of a known genetic element, (b) fragmenting the digital sequence of step (a) into a plurality of suitable complementary oligomeric building blocks that can assemble to form a nucleic acid double-stranded hybrid, (c) synthesizing the plurality of oligomeric building blocks by parallel synthesis steps, wherein each oligomeric building block is synthesized on a different area of a common support, (d) detaching the oligomeric building blocks from the support, (e) bringing the oligomeric building blocks into contact with one another, (f) linking the oligomeric building blocks enzymatically to form the variant of the known genetic element, (g) introducing the variant directly into a target cell and observing a result, and (h) comparing the result obtained in step (g) with a reference result of the known genetic element.