Patent ID: 9683013
Date: 2017-06-20
CPC Classifications: B01D,B01J,C07K

Claim:
1. A method of separating one or more immunoglobulin containing proteins from a liquid, the method comprising, (a) contacting the liquid with a separation matrix comprising ligands immobilised to a support, said ligands comprises one or more monomers having the sequence of SEQ. ID. NO. 2, wherein in at least one of the monomers, the Asparagine at the position N28 of SEQ. ID. NO. 2 has been substituted with another amino acid X to give a substituted monomer having a sequence of SEQ ID No.: 9; (b) allowing said immunoglobulin containing proteins to adsorb to the matrix by interaction with the ligands; (c) an optional step of washing the immunoglobulin containing protein adsorbed matrix; and (d) recovering said immunoglobulin containing proteins by contacting the matrix with an eluent which releases the proteins from the ligand at an elution pH that is higher than the elution pH when using a parental ligand that has the same sequence of said ligand except for said N28X substitution(s) under the same elution condition.