Patent ID: 9701949
Date: 2017-07-11
CPC Classifications: C12N,C12Y

Claim:
1. A modified amadoriase comprising an amino acid sequence having an identity of 90% or more with SEQ ID NO: 1, and comprising a modification as follows: (a) has three amino acid residues deleted from the carboxyl terminal, or has an amino acid substitution as set forth in any of the following (b) to (j): (b) the amino acid at the position corresponding to alanine at position 151 is substituted with another amino acid, (c) the amino acid at the position corresponding to phenylalanine at position 43 is substituted with another amino acid, (d) the amino acid at the position corresponding to histidine at position 53 is substituted with another amino acid, (e) the amino acid at the position corresponding to phenylalanine at position 267 is substituted with another amino acid, (f) the amino acid at the position corresponding to threonine at position 350 is substituted with another amino acid, (g) the amino acid at the position corresponding to alanine at position 185 is substituted with another amino acid, (h) the amino acid at the position corresponding to glutamic acid at position 196 is substituted with another amino acid, (i) the amino acid at the position corresponding to serine at position 299 is substituted with another amino acid, or (j) the amino acid at the position corresponding to valine at position 323 is substituted with another amino acid; wherein residual activity (%) following a heat treatment at 55° C. for 30 minutes at pH 7.0 is improved in comparison with amadoriase having an amino acid sequence of SEQ ID NO: 1 or an amino acid sequence having an identity of 90% or more with SEQ ID NO: 1 prior to being modified.