Patent ID: 9631010
Date: 2017-04-25
CPC Classifications: C07K,G01N

Claim:
1. An aflatoxin B1 nanobody immunosorbent, comprising: a solid phase carrier; and an aflatoxin nanobody coupled to the solid phase carrier, wherein the aflatoxin nanobody consists of aflatoxin B1 nanobody 2014AFB-G15, wherein the aflatoxin B1 nanobody 2014AFB-G15 comprises the amino acid sequence of SEQ ID NO:7, encoded by the nucleic acid sequence of SEQ ID NO:8; wherein the aflatoxin B1 nanobody 2014AFB-G15 comprises further three complementary determining regions of: CDR1 consisting of amino acid sequence of SEQ ID NO: 1; CDR2 consisting of amino acid sequence of SEQ ID NO:2; and CDR3 consisting of amino acid sequence of SEQ ID NO:3; wherein the CDR1 is encoded by the nucleic acid sequence of SEQ ID NO:4; the CDR2 is encoded by the nucleic acid sequence of SEQ ID NO:5; and the CDR3 is encoded by the nucleic acid sequence of SEQ ID NO:6; and wherein the aflatoxin B1 nanobody immunosorbent specifically binds aflatoxin B1.