Patent ID: 9546399
Date: 2017-01-17
CPC Classifications: C12Q

Claim:
1. A method for detecting a duplicate sequencing read from a population of sample sequencing reads comprising: a) ligating an adaptor to a 5′ end of each nucleic acid fragment of a plurality of nucleic acid fragments from one or more samples, wherein the adaptor comprises: b) amplifying the adapter-nucleic acid fragment ligated products; c) generating a population of sequencing reads from amplified adapter-nucleic acid fragment ligated products; and d) identifying a duplicate sequencing read as the sequencing read comprising the same identifier site and nucleic acid fragment as another sequencing read in the population of sequencing reads; wherein the indexing site is unique amongst a subset of the plurality of nucleic acid fragments and is an index for multiple polynucleotides; and wherein the sequence of the identifier site is variable in sequence content in a plurality of adaptors.