Patent ID: 9670504
Date: 2017-06-06
CPC Classifications: C12N

Claim:
1. A transgenic system for reversibly immortalizing quiescent cells, comprising a non-viral, circular non-integrating transgene, wherein said transgenic system comprises a control region (1) and a regulation region (2), whereby said control region (1) comprises a transcriptional regulation system operably linked to a constitutive promoter sequence and said regulation region (2) comprises: a) an RNA interference sequence which prevents translation of a G1-tumour suppressor messenger RNA and which is operably linked downstream of a first inducible promoter sequence; b) a single immortalizing gene consisting of a telomere length maintenance gene sequence which is operably linked downstream to a second inducible promoter sequence; and c) a Scaffold/Matrix attachment region (S/MAR) element positioned downstream in frame with said telomere length maintenance gene sequence and controlled by the second inducible promoter sequence.