Patent ID: 9675646
Date: 2017-06-13
CPC Classifications: A61F,A61K,A61L,C12N

Claim:
1. A method of generating a tubular, bioengineered, gastrointestinal tissue structure comprising: obtaining smooth muscle cells; culturing a first population of the smooth muscle cells to form a first annular smooth muscle cell construct of circumferentially oriented s culturing a second population of the smooth muscle cells to form a second annular smooth muscle cell construct of circumferentially oriented smooth muscle cells; joining the first and second annular smooth muscle cell constructs together around a tubular scaffold, wherein the tubular scaffold comprises chitosan; and culturing the constructs and scaffold in a growth media until a gastrointestinal tissue structure is achieved that exhibits a travelling wave of contractions through the first and second annular smooth muscle cell constructs in response to a contractile stimulus.