Patent ID: 9663779
Date: 2017-05-30
CPC Classifications: C12N

Claim:
1. A method of purifying nucleic acids from a nucleic acid-containing sample, the method comprising: (a) contacting the nucleic acid-containing sample with a nucleic acid-binding phase comprising nucleic acid-binding groups covalently bound to a support, the nucleic acid-binding groups having one or more protonatable groups having a pKa from 9 to 12; (b) binding the nucleic acids to the nucleic acid-binding phase at a binding pH which is more than one pH unit below the pKa of at least one of the one or more protonatable groups; and (c) eluting the nucleic acids at an eluting pH which is above the binding pH, but one, or more than one, pH unit below the pKa of at least one of the one or more protonatable groups,