Patent ID: 9701956
Date: 2017-07-11
CPC Classifications: A61K,C12N

Claim:
1. A method for the in vitro production of DNA minicircles having between 80 base pairs and less than 250 base pairs, said method comprising steps of: a) providing nicked double-stranded oligodeoxynucleotides blunt-ended substrates having at least one phosphorylated 5′ end, wherein the nicked double-stranded oligodeoxynucleotides substrates provided comprise between 80 base pairs and less than 250 base pairs; b) performing a ligase-mediated circularization on a reaction mixture comprising the said nicked double-stranded oligodeoxynucleotides substrates and a DNA bending protein, wherein said DNA bending protein is a non-sequence specific HMBG protein; and c) obtaining DNA minicircles having between 80 base pairs and less than 250 base pairs.