Patent ID: 9670482
Date: 2017-06-06
CPC Classifications: A61K,C07K,C12N,C40B,G01N

Claim:
1. A multispecific peptide ligand comprising: (a) a nucleic acid encoding the multispecific polypeptide; (b) the multispecific peptide displayed on a phage display system, wherein the multispecific polypeptide comprises the sequence (X)IC(X)mC(X)nC(X)o (SEQ ID No. 26), wherein C represents the amino acid cysteine, X represents a random amino acid, m and n are numbers between 1 and 20 defining the length of intervening polypeptide segments and I and o are numbers between 0 and 20 defining the length of the flanking polypeptide segments; (c) the multispecific peptide ligand encoded by the nucleic acid is linked to the nucleic acid by the phage; and (d) the multispecific peptide ligand is attached to tris-(bromomethyl)benzene (TBMB) by three discrete covalent bonds between said TBMB and said multispecific peptide ligand via said C amino acid residues, and form at least one loop which comprises a sequence of two or more amino acids subtended between two of said C amino acid residues, wherein the multispecific peptide ligand is capable of binding to two or more targets, thus forming the multispecific peptide ligand.