Patent ID: 9677125
Date: 2017-06-13
CPC Classifications: C12Q

Claim:
1. A method of detecting at least three sets of different targets in a biological sample via simultaneous addition of at least three sets of different target-binding probes followed by their sequential detection, the method comprising: (a) simultaneously contacting the at least three sets of different targets with the at least three sets of different target-binding probes to form a plurality of target-bound probes; (b) covalently attaching each of the plurality of target-bound probes of step (a) to the corresponding target or to a molecule that is located in the vicinity of the corresponding target in the biological sample to form a plurality of covalently attached probes; and (c) sequentially detecting the plurality of covalently attached probes of step (b) to sequentially detect each set of different target, wherein the sequential detection comprises: