Patent ID: 9657286
Date: 2017-05-23
CPC Classifications: B01L,C12N,C12Q,G01N

Claim:
1. A pre-processing integrated capillary electrophoresis device comprising: a mount table; a pre-processing/electrophoresis integrated cartridge which mounts on the mount table, the cartridge including a plurality of block structures corresponding to a step of extracting a nucleic acid from a biological sample, a step of performing a PCR reaction on a target region of the nucleic acid, and a step of performing a cycle sequence reaction on the nucleic acid that is amplified, the block structures including: (1) a first block having a reaction tank into which the biological sample is injected, a reagent tank that stores a reagent for use in a reaction with the biological sample, a plurality of flow channels that connect the tanks, a plurality of control valves arranged in the flow channels, and a flow channel that connects a preceding reaction tank in a preceding one of the block structures located at a preceding stage of the first block and a next reaction tank in a next one of the block structures located at a next stage, and (2) a second block having a phoretic solution tank that stores a phoretic solution, a cathode buffer solution tank that stores a buffer solution containing an electrolyte for use in capillary electrophoresis, a wash solution tank that stores a wash solution for washing a tip end of a capillary to avoid contamination between samples, a flow channel that connects a preceding carrier tank in a preceding one of the block structures located at a preceding stage of the second block and the phoretic solution tank, and a control valve for the flow channel; a temperature adjustment mechanism for adjusting a temperature of a solution in the cartridge; a capillary electrophoresis mechanism; and a controller configured to control opening and closing of the control valves to control liquid transfer in and between the first and second blocks, and control an electrophoresis operation by controlling the capillary electrophoresis mechanism, wherein at least one of the step of extracting a nucleic acid from the biological sample, the step of performing a PCR reaction on a target region of the nucleic acid, or the step of performing a cycle sequence reaction on the nucleic acid that is amplified, and a capillary electrophoresis step of analyzing the nucleic acid are all executed automatically.