Patent ID: 9701973
Date: 2017-07-11
CPC Classifications: C12N

Claim:
1. A method for transforming a plastid of a maize cell, said method comprising: (a) culturing an immature embryo of a maize plant in a callus induction medium in the absence of light to obtain a dark-grown embryogenic callus; (b) culturing said dark-grown embryogenic callus in a medium comprising one or more plant growth regulators in the presence of light to obtain a greening embryogenic callus; (c) introducing an exogenous DNA molecule of interest comprising an expression cassette into at least one cell of said greening embryogenic callus, wherein said expression cassette is expressible in a plastid and becomes integrated into a plastid genome of said at least one cell; and (d) selecting for at least one plastid transformed maize cell from said greening embryogenic callus comprising said expression cassette.