Patent ID: 9731288
Date: 2017-08-15
CPC Classifications: B01J,C07K,C08F

Claim:
1. A method of separating a target molecule from one or more impurities in a sample, where the method comprises the steps of: (a) providing a sample comprising a target molecule and one or more impurities; (b) contacting the sample with a soluble stimulus responsive polymer selected from the group consisting of benzyl modified polyallylamine; diphenyl modified polyallylamine; dichlorobenzyl modified polyallylamine; chlorobenzyl modified polyallylamine; hexanoic acid and tert-butyl modified polyallylamine; phenylbenzyl modified polyallylamine; benzyl modified polyethyleneimine; and benzyl modified polyvinylamine, wherein the stimulus responsive polymer is capable of binding and precipitating one or more impurities following the addition of a multivalent ion stimulus; and (c) adding a multivalent ion stimulus selected form phosphate or citrate to the sample to precipitate complex of polymer and one or more impurities out of the sample;