Patent ID: 9663832
Date: 2017-05-30
CPC Classifications: C12Q

Claim:
1. A method for determining zygosity of a canola plant comprising a fad-3c gene, said method comprising: obtaining a sample of genomic DNA from a canola plant; hybridizing the sample of genomic DNA with a first primer and a second primer, wherein said first primer and second primer comprise SEQ ID NO: 2 and SEQ ID NO: 3; subjecting said sample to polymerase chain reaction (PCR) conditions, wherein an amplicon is produced; allowing each of a first probe and a second probe to hybridize to the amplicon for a period of time and at a temperature between 50-70 degrees Celsius, wherein said first probe and second probe comprise SEQ ID NO: 5 and SEQ ID NO: 4, wherein each of said first probe and second probe is labeled with a fluorescent dye and a quencher; increasing said temperature after the period of time; measuring florescence of the first probe, the second probe, or a combination thereof; and determining zygosity of said canola plant.