Patent ID: 9738935
Date: 2017-08-22
CPC Classifications: A61P,C12Q

Claim:
1. A method of detecting a 2236_2248>ACCC mutation in the epidermal growth factor receptor (EGFR) sequence of SEQ ID NO:1 in a sample comprising nucleic acid from a human, comprising: (a) contacting the nucleic acid in the sample with at least one allele-specific primer having a sequence selected from SEQ ID NOs: 23-25; (b) incubating the nucleic acid and at least one allele-specific primer and amplifying the target nucleic acid, wherein amplification results from extension of the allele-specific primer if the target nucleic acid contains a 2236_2248>ACCC mutation in the EGFR sequence of SEQ ID NO:1, and (c) detecting the 2236_2248>ACCC mutation in the EGFR sequence of SEQ ID NO: 1 by detecting the amplification in step (b).