Patent ID: 9709549
Date: 2017-07-18
CPC Classifications: A61M,B01D,C12N,G01N

Claim:
1. A method for processing biological material comprising: providing a biological material comprising red blood cells and nucleated cells in a vessel; adding an aggregating agent to the material in the vessel and allowing the material to separate into two or more distinct layers of submaterials, wherein one of the distinct layers of submaterials comprises a layer of red blood cells and another distinct layer comprises a sample mixture in which a target retentate is present; extracting the layer of red blood cells from the vessel via a pick up line having a distal end located within the vessel at a level at which the layer of red blood cells settles during operation; automatically transporting the sample mixture remaining in the vessel to a filtration device located downstream from the pickup line and separated from the vessel by a valve; separating the sample mixture using the filtration device into the target retentate which is captured by the filtration device, a sample remainder which is directed to the vessel for recirculation, and a waste filtrate; recirculating the sample remainder with the sample mixture in the vessel; stopping circulation of the sample mixture and recirculation of the sample remainder once the predetermined concentration of nucleated cells within the vessel is reached; and collecting a resulting target retentate from the filtration device into a target retentate receptacle.