Patent ID: 9650672
Date: 2017-05-16
CPC Classifications: C12N,C12P,C12Q

Claim:
1. A composition comprising: a triplet state quencher, wherein the triplet state quencher is a trivalent lanthanide ion, a fluorescently labeled or fluorogenic nucleotide analog, and a recombinant DNA polymerase to which the triplet state quencher is noncovalently bound, wherein the recombinant polymerase comprises an amino acid sequence that is at least 90% identical to SEQ ID NO:1 or SEQ ID NO:21, wherein the nucleotide analog serves as a substrate for the polymerase, wherein the nucleotide analog does not comprise the triplet state quencher, and wherein the triplet state quencher serves as an acceptor for the excited triplet state of the fluorescently labeled analog or of the fluorogenic analog's product.