Patent ID: 9725705
Date: 2017-08-08
CPC Classifications: A61P,C07K,C12N,C12P,C12Y

Claim:
1. A process for purifying a botulinum toxin comprising the step of culturing Clostridium botulinum bacteria in an animal protein free (APF) culture medium and sequentially contacting a plurality of chromatography columns with an aqueous medium containing a botulinum toxin, thereby obtaining a purified botulinum toxin; wherein the plurality of chromatography columns comprise at least a first chromatography column and a second chromatography column; wherein the first chromatography column is an ion exchange chromatography column, and the second chromatography column is a hydrophobic interaction chromatography column; wherein the botulinum toxin is a botulinum toxin complex type A comprising a heavy chain and a light chain; and wherein the process is an animal protein free (APF) process.