Patent ID: 9719984
Date: 2017-08-01
CPC Classifications: G01N

Claim:
1. A method of determining an antibody profile in a test sample comprising a bodily fluid from a mammalian subject, wherein the antibody is a biological marker of a disease state or disease susceptibility and wherein it is not known whether the test sample comprises the antibody, the method comprising: (a) contacting the test sample with a plurality of different amounts of an antigen specific for the antibody, (b) detecting the amount of specific binding between the antibody and the antigen and (c) plotting or calculating a curve of the amount of the specific binding versus the amount of antigen for each amount of antigen used in step (a), and (d) comparing the curve of the test sample with a control curve, wherein a difference in the test sample curve when compared with the control sample curve indicates the presence of a disease state or disease susceptibility in the subject and wherein the difference provides the profile of antibody in the subject either prior to the onset of disease or during the course of disease.