Patent ID: 9572876
Date: 2017-02-21
CPC Classifications: A61K,C12N,C12Y

Claim:
1. A recombinant paramyxovirus composition comprising a nucleic acid molecule which encodes large RNA polymerase (L) protein having a defective mRNA cap MTase domain in a paramyxovirus viral genome, wherein the paramyxovirus viral genome is human respiratory syncytial virus (hRSV); and, wherein the nucleic acid molecule which encodes large RNA polymerase (L) protein having a defective mRNA cap MTase domain carries at least one mutation in a S-adenosyl methionine (SAM) binding site; wherein the at least one mutation in the SAM binding site is in a G×G×G . . . D/E/W site in an amino acid sequence corresponding to amino acid positions 31-90 of SEQ ID NO: 3, wherein the mutations comprise: at position 31, A×G×G . . . D; at position 33, G×A×G . . . D; at position 35, G×G×A . . . D; and, at position 90, G×G×G . . . A.