Patent ID: 9670520
Date: 2017-06-06
CPC Classifications: B01D,C07K,C12M,C12N,C12P

Claim:
1. Process for the culturing of cells in a reactor in suspension in a cell culture medium, comprising: circulating a cell culture through a separation system, wherein the cell culture comprises eukaryotic cells, a biological substance produced by the cells, and a cell culture medium; wherein the biological substance is an IgG; wherein at least one cell culture medium component is fed to the cell culture; wherein the cell culture is circulated through the separation system in a flow substantially parallel to a surface of said separation system resulting in a liquid outflow and a flow wherein cell culture contents are retained or fed back into the reactor; wherein the separation system comprises a filter having a pore size characterized by a molecular weight cut-off smaller than the molecular weight of the biological substance to separate the biological substance from substances having a lower molecular weight than the biological substance; wherein the liquid outflow consists essentially of components having molecular weight lower than that of the biological substance and the flow wherein cell culture contents are retained or fed back into the reactor consists essentially of components having molecular weight equal to or higher than that of the biological substance; wherein the cell culture is removed at least once from the reactor, and wherein the biological substance is harvested from the cells and/or from the cell culture.