Patent ID: 9719068
Date: 2017-08-01
CPC Classifications: C12N

Claim:
1. An in vitro method of inducing formation of mammalian intestinal tissue, comprising the steps of: a) contacting mammalian definitive endoderm cells with 100 ng/ml of FGF4 or higher and 100 ng/ml or higher Wnt3a for at least 96 hours to obtain posterior definitive endoderm cells, b) culturing the posterior definitive endoderm cells of step (a) to obtain 3-dimensional spheroids, c) embedding the 3-dimensional spheroids of step (b) in a basement membrane-like matrix to obtain intestinal tissue; and d) maintaining said embedded intestinal tissue in a media comprising at least 50 ng/mL of EGF, wherein said maintaining produces a stable mammalian intestinal tissue.