Patent ID: 9683251
Date: 2017-06-20
CPC Classifications: A61K,C12N,C12P,C12Y

Claim:
1. A method for producing a nucleic acid encoding a target protein having enzymatic activity, the method comprising the steps of (a) providing a first mixture in the absence of compartmentalization of a plurality of RNA or DNA molecules comprising at least one molecule encoding the target protein; (b) generating the target protein in vitro from the first mixture to form a second mixture in the absence of compartmentalization of a plurality of RNA-protein or DNA-protein complexes in which the RNA or DNA molecules are noncovalently or covalently bound to the complex; then (c) after (a) and (b), separating the plurality of complexes into initial emulsified in vitro compartments, wherein at least a plurality of the compartments contain no more than one complex; (d) subjecting the complexes to reaction conditions that allow target protein enzymatic activity; and (e) selecting nucleic acid encoding the target protein on the basis of quantification of the enzymatic activity associated therewith.