Patent ID: 9580736
Date: 2017-02-28
CPC Classifications: B01L,C12N,C12P,C12Q

Claim:
1. A method for producing one or more polynucleotides of interest, the method comprising: obtaining a plurality of RNAs associated with one or more samples, wherein adding an adapter molecule to the RNAs associated with the sample, wherein the adapter molecule is generated using an enzymatic reaction and comprises a universal priming sequence, a barcode sequence, and a binding site, wherein the adapter molecule is generated by contacting a template molecule with one or more enzymes and the template molecule is a DNA molecule comprising an RNA polymerase (RNAP) promoter, and the one or more enzymes include an RNA polymerase; and incorporating the barcode sequence into one or more polynucleotides associated with the sample, thereby producing the one or more polynucleotides of interest.