Patent ID: 9725500
Date: 2017-08-08
CPC Classifications: A61K,A61P,C07K,G01N

Claim:
1. A method for making antibody conjugates for use in high throughput screening assays comprising the steps of: providing a plurality of antibody-containing samples that vary with respect to antibody quantity and antibody sequence provided that, in a majority of the antibody-containing samples, substantially all of the antibody present in each of the samples is of the same sequence; immobilizing the antibodies of the antibody-containing samples on a solid support to provide a plurality of antibody-containing samples comprising immobilized antibodies; fully reducing the reducible disulfide bonds of the immobilized antibodies to provide a plurality of samples comprising reduced immobilized antibodies; wherein the reduction is selective for reducible disulfide bonds; reacting the reduced immobilized antibodies with capping agent, drug or drug-linker, and optionally a detection agent to provide immobilized antibody conjugates; wherein the capping agent, drug or drug-linker, and optional detection agent selectively react with reactive thiols, the capping agent, drug or drug-linker, and optional detection agent are provided in molar excess, and the ratio of capping agent, drug or drug linker, and optional detection agent is selected so as to achieve a desired level of drug loading; and eluting the immobilized antibody conjugates to provide a plurality of antibody conjugate samples.