Patent ID: 9556274
Date: 2017-01-31
CPC Classifications: A61K,A61P,C07K

Claim:
1. A polyvalent bispecific antibody (PBA) comprising two pairs of polypeptide chains, wherein each pair comprises at least one anti-IGF-1R binding site and at least one anti-ErbB3 binding site; and (i) the anti-IGF-1R binding site comprises (a) a heavy chain variable (VH) domain comprising a set of three VH Complementarity Determining Regions (CDRs) comprising VHCDR1 (amino acid numbers 26-35), VHCDR2 (amino acid numbers 51-66), and VHCDR3 (amino acid numbers 99-111) of a heavy chain comprising the amino acid sequence selected from the group consisting of SEQ ID NOs: 9, 12-31, 226, and 384-385; and (b) a light chain variable (VL) domain comprising a set of three VLCDRs comprising VLCDR1 (amino acid numbers 24-34), VLCDR2 (amino acid numbers 50-56), and VLCDR3 (amino acid numbers 89-97) of a light chain comprising the amino acid sequence selected from the group consisting of SEQ ID NOs:3, 32, 33, 36-133, 204, and 386-387, and (ii) the anti-ErbB3 binding site comprises (a) a heavy chain variable (VH) domain comprising a set of three VH CDRs comprising VHCDR1 (amino acid numbers 26-35), VHCDR2 (amino acid numbers 51-66), and VHCDR3 (amino acid numbers 99-111) of a heavy chain comprising the amino acid sequence selected from the group consisting of SEQ ID NOs:5, 134-141, 143-165, and 388, or VHCDR1 (amino acid numbers 492-501), VHCDR2 (amino acid numbers 517-532), and VHCDR3 (amino acid numbers 565-577) of a molecule comprising the amino acid sequence of SEQ ID NO:226; and (b) a light chain variable (VL) domain comprising a set of three VLCDRs comprising VLCDR1 (amino acid numbers 23-33), VLCDR2 (amino acid numbers 49-55), and VLCDR3 (amino acid numbers 88-98) of a light chain comprising the amino acid sequence selected from the group consisting of SEQ ID NOs:7, 166-173, and 176-200, or VLCDR1 (amino acid numbers 634-644), VLCDR2 (amino acid numbers 660-666), and VLCDR3 (amino acid numbers 699-709) of a molecule comprising the amino acid sequence of SEQ ID NO:226.