Patent ID: 9580760
Date: 2017-02-28
CPC Classifications: C12N,C12Q

Claim:
1. A method of detecting the presence or absence of a mold having hyphae, comprising: (a) applying a sample suspected of containing the mold to a volume of liquid growth medium containing a porous substrate attached to a shaft, wherein said porous substrate attached to a shaft is not used to collect the sample prior to step a); (b) allowing growth of said mold to occur in said liquid growth medium of step a) and for said mold to attach onto said porous substrate attached to a shaft; (c) transferring said mold attached to said porous substrate attached to a shaft from step b) to a phosphate buffer solution by: d) mechanically fragmenting said mold in said volume of phosphate buffer solution from step c) by subjecting said porous substrate attached to a shaft with attached mold to a shear force to disrupt connections between segments of the hyphae and produce fragmented mold hyphae segments; e) associating said fragmented mold hyphae segments sample of step d) with a labeling agent; and f) directly detecting the presence or absence of said mold hyphae segments with said labeling agent of step e) by flow cytometry;