Patent ID: 9296781
Filing Date: 2016-03-29
CPC Classification: A61K,A61Q,C07K,C08H,C08L

Claim Text:
1. A method for isolation of one or more connective tissue proteins from one or more fat containing tissues comprising the steps of: harvesting the fat containing tissue obtained by liposuction, abdominoplasty, or any other fat removal techniques, in a jacketed vessel comprising: a sonic probe, an outer jacket and a mesh filter, wherein the sonic probe and the mesh filter are inside the jacketed vessel, wherein the jacketed vessel further comprises: a first inlet that allows passage of the one or more fat containing tissue into an interior of the jacketed vessel, which comprises a cooling jacket comprising a cooling inlet and a cooling outlet, wherein the sonic probe in the jacketed vessel capable of generating an acoustic pulses capable of disrupting the fat containing tissue in the jacketed vessel, a mesh filter capable of separating stromal vascular lipids in the jacketed vessel by filtration and in fluid communication with a first outlet, a second inlet in fluid communication with the interior of the jacketed vessel for a washing fluid during the filtration, and a second outlet in fluid communication with the interior of the jacketed vessel for isolation of isolated connective tissue protein by the filtration; flowing the fat containing tissue into the jacketed vessel, wherein the jacketed vessel is an acoustically permeable closed system; exposing directly the fat containing tissue to one or more acoustic pulses in the jacketed vessel to separate the one or more connective tissue proteins from a stromal vascular fraction (SVF), wherein the SVF comprises lipids, cells, or any other extracellular elements pass through the mesh filter; thereby isolating the separated connective tissue proteins from the fraction that did not traverse the mesh filter.