Patent ID: 9481870
Filing Date: 2016-11-01
CPC Classification: C12N,C12P,C12Y,Y02P

Claim Text:
1. A DNA consisting of a nucleotide sequence encoding a polypeptide selected from the group consisting of: (a) a polypeptide consisting of an amino acid sequence that is identical to the amino acid sequence of SEQ ID NO: 1 except for a deletion, a substitution, an insertion and/or an addition of 1 to 20 amino acids in SEQ ID NO: 1, wherein said polypeptide acts on 1-benzyl-3-pyrrolidinone in the presence of an amino group donor to generate (S)-1-benzyl-3-aminopyrrolidine with optical purity of 93% enantiomeric excess or more; and (b) a polypeptide consisting of an amino acid sequence that is identical to the amino acid sequence of SEQ ID NO: 1 except for a deletion, a substitution, an insertion and/or an addition of 1 to 20 amino acids in SEQ ID NO: 1, wherein said polypeptide acts on 1-benzyl-3-pyrrolidinone in the presence of an amino group donor to generate (S)-1-benzyl-3-aminopyrrolidine with optical purity of 93% enantiomeric excess or more; and wherein said polypeptide (i) exhibits activity for (S)-1-phenethylamine, benzylamine, and 2-butylamine as amino group donors and does not substantially exhibit activity for β-alanine and 4-aminobutyric acid, (ii) exhibits activity for pyruvic acid and activity for glyoxalic acid as amino group receptors; and/or (iii) retains 10% or more of residual activity after 2 hours of treatment with a solution having a concentration of 80% v/v of 1-propanol, 2-propanol, or acetone compared to the enzymatic activity prior to the treatment.