Patent ID: 9404153
Filing Date: 2016-08-02
CPC Classification: C12Q

Claim Text:
1. A kit for the detection of Bacillus anthracis genomic DNA target nucleic acid, wherein the kit comprises: (1) a pair of primers capable of amplifying the target nucleic acid, the pair of primers comprising a limiting primer and a non-limiting primer, the molar ratio between the limiting primer and the non-limiting primer meets the demand of an asymmetric PCR reaction, and the pair of primers are stored independently or in a mixture, wherein the limiting primer sequence consists of the sequence of SEQ ID NO: 1, the non-limiting primer sequence consists of the sequence of SEQ ID NO: 2, and the molar ratio between the limiting primer and the non-limiting primer is 1:10-100; and (2) a probe-containing solution independently stored from the pair of primers or mixture thereof, wherein a probe in the probe-containing solution comprising two conjugates, a first conjugate of the two conjugates comprises a first gold nano-particle having a diameter of 5-100 nm and an oligonucleotide with a sequence consisting of the sequence of SEQ ID NO: 3, a second conjugate of the two conjugates comprises a second gold nano-particle having a diameter of 5-100 nm and an oligonucleotide with a sequence consisting of the sequence of SEQ ID NO: 4, the molar ratio between the first conjugate and the second conjugate is 1:0.8-1.2, and the first gold nano-particle and the second gold nano-particle are identical.