Patent ID: 9441278
Filing Date: 2016-09-13
CPC Classification: C12Q

Claim Text:
1. A method of genotyping an individual head of cattle comprising steps of: a. genotyping a DNA sample obtained from said individual wherein said genotyping utilizes PCR SNaPshot protocol, said protocol comprising a first PCR step (PCR1) and a second PCR step (PCR2), further wherein said PCR1 step includes adding a first primer set consisting of PCR1 primers of SEQ ID No. 1-30 to PCR1 reaction comprising said DNA sample to yield PCR1 products, and said PCR2 step includes adding extension primers consisting of PCR2 primers of SEQ ID No. 31-45 to yield PCR2 products, said genotyping is performed by multiplexing single nucleotide polymorphisms (SNPs) by amplifying in concert said PCR1 products in a single tube, followed by multiplexing said SNPs by amplifying in concert PCR2 products in the tube; b. producing amplicons from said PCR1 reaction comprising said DNA sample and said first primer set to yield said PCR1 products; c. using said PCR1 products as templates to said extension primers to yield said PCR 2 products; d. separating said PCR2 products on a capillary sequencer, thereby obtaining SNP profile results; e. recording said SNP profile results into a databank; and, f. comparing said SNP profile results with SNP profiles from said databank using data mining software to obtain matching profiles.