Patent ID: 9476025
Filing Date: 2016-10-25
CPC Classification: C12N

Claim Text:
1. A method of studying three-dimensional cell growth, comprising the steps of: providing a plurality of microcarriers, wherein the microcarriers are magnetic microcarriers; providing an upper and a lower diamagnetic plate; providing an adjustable platform, whereby the upper and lower diamagnetic plates are adjustably spaced; adhering at least one cell to the microcarriers in a gas-permeable tissue culture bag; placing the tissue culture bag between the upper diamagnetic plate and the lower diamagnetic plate; and placing a magnet above the upper diamagnetic plate relative to the tissue culture bag whereby the growing cells are levitated by adjustment of the platform to the appropriate height and are held in suspension via natural magnetic forces stabilized by the diamagnetic plates, thereby allowing the study of three-dimensional cell growth in suspension in a constant and nonrandomized environment.