Patent ID: 9453847
Filing Date: 2016-09-27
CPC Classification: A61K,C07K,C12N,C12Y,G01N,G16H,Y02A

Claim Text:
1. A method of measuring cellular uptake of glucocerebrosidase (GCB) into a mammalian cell, the method comprising: contacting a mammalian cell that stably expresses on its surface mannose receptor, C type 1 (MRC1) with GCB; and measuring the amount of GCB in the cell, wherein the cell comprises a nucleic acid molecule comprising a nucleic acid sequence encoding a MRC1 polypeptide having an amino acid sequence that is at least 99% identical to SEQ ID NO:4, wherein the nucleic acid sequence encoding the MRC1 polypeptide comprises optimized codons so that at least 15% of the nucleic acid residues within positions 1 to 2067 of the nucleic acid sequence are different from positions 1 to 2067 of SEQ ID NO: 1, and wherein the optimized codon refers to the following codons: Ala (gcc); Arg (aga); Asn (aac); Asp (gac); Cys (ugc); Gln (cag); Gly (ggc); His (cac); Ile (auc); Leu (cug); Lys (aag); Pro (ccc); Phe (uuc); Ser (agc); Thr (acc); Tyr (uac); Glu (gag); and Val (gug).