Patent ID: 9376701
Filing Date: 2016-06-28
CPC Classification: C07K,C12N,C12P

Claim Text:
1. A method for producing a heterologous protein comprising: A) culturing a coryneform bacterium having a genetic construct for secretory expression of a heterologous protein; B) allowing the bacterium to produce and secrete the heterologous protein, and C) collecting the heterologous protein, wherein the genetic construct comprises: i) a promoter sequence that functions in the coryneform bacterium, ii) a first nucleic acid sequence coding for a signal peptide that functions in the coryneform bacterium, wherein said first nucleic acid sequence is ligated downstream from the promoter sequence, and iii) a second nucleic acid sequence coding for a fusion protein having: wherein said second nucleic acid sequence is ligated downstream from the first nucleic acid sequence coding for the signal peptide, and wherein the amino acid sequence comprising Gln-Glu-Thr does not consist of an amino acid sequence consisting of the amino acid residues at positions 1 to 14 or positions 1 to 38 of SEQ ID NO: 96.