Patent ID: 9376463
Filing Date: 2016-06-28
CPC Classification: C07K

Claim Text:
1. A process for purification of a target biomolecule, comprising: (a) contacting a target biomolecule and, a dual affinity polypeptide in solution to form a binding of the target biomolecule to the dual affinity polypeptide having an equilibrium dissociation constant K D,t , (b) subsequently contacting the target biomolecule bound dual affinity polypeptide, and a solid support comprising a catching ligand to form a binding of the dual affinity polypeptide to the solid support having an equilibrium dissociation constant K D,s , wherein the ratio between the equilibrium dissociation constants of the dual affinity polypeptide [K D,t /K D,s ] is at least 10 1 at standard conditions, and (c) recovering the target biomolecule by elution while the dual affinity polypeptide remains immobilized on the solid support.