Patent ID: 9493499
Filing Date: 2016-11-15
CPC Classification: C07H

Claim Text:
1. A method of making a composition comprising a modified nucleotide sugar covalently linked to a polymeric modifying group, said polymeric modifying group comprising at least one linear or branched poly(alkylene oxide) moiety, said method comprising: (i) contacting a reaction mixture comprising said modified nucleotide sugar with a Q-sepharose anion exchange resin; (ii) eluting said modified nucleotide sugar from said Q-sepharose anion exchange resin using a bicarbonate buffer, wherein said eluting comprises a first step wherein said buffer comprises between about 0.01 mM and about 10 mM of bicarbonate and a second step wherein said buffer comprises between about 10 mM and about 30 mM of bicarbonate, thereby forming an eluate fraction comprising said modified nucleotide sugar; and (iii) desalting said eluate fraction, wherein said method does not comprise ultrafiltration prior to step (i), thereby forming said composition, wherein said modified nucleotide sugar comprises cytidine-monophospho-sialic acid (CMP-SA), wherein said method provides said modified nucleotide sugar in a purity between about 80% and about 100% (w/w) and wherein the linear or branched poly(alkylene oxide) moiety has a molecular weight in the range of 10-40 kDa.