Patent ID: 9480549
Filing Date: 2016-11-01
CPC Classification: A61F,A61K,A61L

Claim Text:
1. A method of manufacturing a multi-layer amnion tissue patch, the method comprising: obtaining a first amnion tissue layer having an epithelial layer, a basement membrane, a compact layer, and a fibroblast layer from which a spongy layer has been removed, the fibroblast layer including a loose network of reticulum, wherein the first tissue layer is treated with a glutaraldehyde exposure process; obtaining a second amnion tissue layer having an epithelial layer, a basement membrane, a compact layer, and a fibroblast layer from which a spongy layer has been removed, the fibroblast layer including a loose network of reticulum, wherein the second tissue layer is not treated with a glutaraldehyde exposure process; compressing the treated first amnion tissue layer with the untreated second amnion tissue layer so as to form the patch, such that the epithelial layers of the first and second amnion tissue layers define first and second opposing outer surfaces of the patch, respectively, wherein a compressed, coupled fibroblast region is disposed between at least a portion of the first and second opposing outer surfaces of the patch, and wherein the compressed, coupled fibroblast region comprises fibers of at least a portion of the first fibroblast layer entangled with fibers of at least a portion of the second fibroblast layer.