Patent ID: 9447463
Filing Date: 2016-09-20
CPC Classification: C12Q

Claim Text:
1. A method of amplifying a nucleic acid sequence in a parallel sequencing technique, said method comprising: a) hybridizing a plurality of target nucleic acid sequences with a plurality of first linear primers which contain (i) a terminal 3′ region having a first random sequence and (ii) a 5′ region having a first universal sequence, b) forming a plurality of extended linear primer products by extending the first linear primers that are hybridized to the target nucleic acid sequence; c) hybridizing the plurality of the extended linear primer products to a plurality of second linear primers which contain (i) a terminal 3′ region having a second random sequence and (ii) a 5′ region having a second universal sequence, d) forming a plurality of double-extended linear primer products by extending the plurality of second linear primers that are hybridized to the plurality of the extended linear primer products.