Patent ID: 9394573
Filing Date: 2016-07-19
CPC Classification: C12Q

Claim Text:
1. A method of amplifying in a sample a methicillin-resistant Staphylococcus aureus (MRSA) which comprises an insertion of a SCCmec cassette within Staphylococcus aureus chromosomal DNA, wherein the SCCmec cassette comprises a mecA variant element, the method comprising: performing on the sample an amplification reaction utilizing an oligonucleotide set consisting of: a. a first oligonucleotide having a nucleic acid sequence that specifically hybridizes to chromosomal b. a second oligonucleotide having a nucleic acid sequence that specifically hybridizes to a mecA variant, wherein each of the first oligonucleotide and the second oligonucleotide is oriented such that, under amplification conditions, if the sample contains the MRSA, the region of the MRSA between the hybridizing site of the first oligonucleotide and the hybridizing site of the second oligonucleotide is amplified as a single amplicon.