Patent ID: 9340822
Filing Date: 2016-05-17
CPC Classification: C12Q,G01N

Claim Text:
1. A method of determining in an individual the presence, identity, and/or severity of a disease or condition associated with abnormal glycan biosynthesis, degradation, or accumulation, the method comprising: (a) generating a first biomarker comprising a glycan residual compound, wherein the first biomarker is generated by treating a population of glycans, in or isolated from a biological sample from the individual, with at least one digesting glycan enzyme, wherein prior to enzyme treatment, the first biomarker is not present in abundance in samples from individuals with the disease or condition relative to individuals without the disease or condition, and wherein the first biomarker is a non-reducing end biomarker; (b) generating a second biomarker comprising a glycan residual compound, wherein the second biomarker is generated by treating a population of glycans, in or isolated from a biological sample from the individual, with at least one digesting glycan enzyme in the same or different digestion step as provided in step (a), wherein prior to enzyme treatment, the second biomarker is not present in abundance in samples from individuals with the disease or condition relative to individuals without the disease or condition, and wherein: (c) using an analytical instrument to detect the presence of and/or measure the amount of the first and second biomarker produced and displaying or recording the presence of or a measure of a population of the first and second biomarkers; and (d) monitoring and/or comparing the amounts of the first and second biomarkers in a biological sample; wherein the presence of and/or measure of the amounts of the first and second biomarkers are utilized to determine the presence, identity, and/or severity of the disease or condition; and wherein the disease or condition is a lysosomal storage disease, cancer, an inflammatory disease, liver disease, bone diseases, an infectious disease, a central nervous system disease, or a cardiovascular disease.