Patent ID: 9506113
Filing Date: 2016-11-29
CPC Classification: C12N,C12P,C12Q

Claim Text:
1. A method for analyzing nucleic acids, the method comprising: a) contacting one or more double stranded nucleic acid inserts comprising single stranded nucleotide overhangs with a plurality of double stranded nucleic acid adaptors, wherein each of said plurality of adaptors have a distinct single stranded adaptor overhang relative to the other adaptors, wherein said adaptor overhangs have random nucleotide sequences; b) ligating said adaptors to said one or more double stranded nucleic acid inserts with a ligase enzyme to generate ligated inserts; and c) analyzing said ligated inserts by sequencing said ligated inserts using a next-generation sequencing technology selected from the group consisting of pyrosequencing, sequencing-by-ligation, sequence-by-synthesis (SBS), nanopore sequencing, and oligonucleotide extension.