Patent ID: 9382307
Filing Date: 2016-07-05
CPC Classification: C07K,G01N

Claim Text:
1. A polypeptide comprising a modified VDR ligand-binding domain (VDR-LBD) and a donor-acceptor pair of fluorophores, wherein said polypeptide comprises at least one mutation causing said modified VDR to have a higher binding affinity to 25-hydroxyvitamin D relative to calcitriol, and wherein said at least one mutation comprises a substitution at amino acid position 274, 147, 150, 227, 230, 233, 234, 271, 275, 286, 288, 300, 309, 313, 418, 237, 143, 278, 305 and 397 of SEQ ID NO: 1.