Patent ID: 9528098
Filing Date: 2016-12-27
CPC Classification: C12N,C12P,C12Y,C13K,Y02E,Y02P

Claim Text:
1. A method for generating glucose, comprising contacting a lignocellulose substrate with an enzyme mixture comprising two or more M. thermophila cellulose hydrolyzing enzymes selected from endoglucanases (EGs), beta-glucosidases (BGLs), Type 1 cellobiohydrolases (CBH1s), Type 2 cellobiohydrolases (CBH2s), and/or glycoside hydrolase 61s (GH61s), to generate glucose and/or cellobiose, wherein at least one of the cellulose hydrolyzing enzymes is endogenous to an M. thermophila fungal cell and wherein the enzyme mixture is characterized in that, when the enzyme mixture is contacted with cellobiose and/or glucose, no more than about 10%, about 15%, or about 20% of the cellobiose and/or glucose produced using the method is oxidized after 10 hours, the method further comprising the step of subjecting the enzyme mixture to a purification process to selectively remove at least one glucose and/or cellobiose oxidizing enzyme from the enzyme mixture.