Patent ID: 9453258
Filing Date: 2016-09-27
CPC Classification: C12Q

Claim Text:
1. A method for determining sequences of polynucleotides, comprising (a) performing a sequencing reaction that comprises at least 50 repeated cycles of (b) detecting signals in the sequencing reaction for the incorporation of three different types of the nucleotide conjugates into the polynucleotides, wherein signals are not detected for a fourth type of the nucleotide conjugates incorporated into the polynucleotides; and (c) determining the incorporation of the fourth type of the nucleotide conjugates based on the detection pattern of the three different types of the nucleotide conjugates into the polynucleotides, thereby determining sequences comprising at least 50 sequential nucleotides of the polynucleotides, wherein the incorporation of the three different types of the nucleotide conjugates is determined from a signal state and wherein the incorporation of the fourth type of the nucleotide conjugates is determined from a dark state.