Patent ID: 9261515
Filing Date: 2016-02-16
CPC Classification: G01N,Y10T

Claim Text:
1. A method for calculating a volume and/or a hemoglobin content of an individual red blood cell in a blood sample, the method comprising: (i) passing a blood sample through a flow cell of a hematology analyzer and directing a light source to the flow cell to generate a plurality of optical data using a plurality of detectors, wherein the plurality of detectors comprises one or more of: an axial light loss (ALL) detector, an intermediate angle scatter (IAS) detector, a depolarized side scatter (DSS) detector, a polarized side scatter (PSS) detector, a green fluorescence (FL (ii) measuring a hemoglobin concentration in the blood sample using a colorimetric transducer; (iii) measuring a change in impedance in the blood sample using an impedance transducer; and (iv) analyzing the optical data, the hemoglobin concentration, and the change in impedance to calculate a volume and/or a hemoglobin content of an individual red blood cell in the blood sample, wherein the volume of an individual red blood cell is calculated using a second order global volume model that comprises an IAS measurement and an ALL measurement of the individual red blood cell, and wherein the hemoglobin content of an individual red blood cell is calculated using a local fit cellular hemoglobin concentration model that comprises an IAS measurement and a PSS measurement of the individual red blood cell.