Patent ID: 9394547
Filing Date: 2016-07-19
CPC Classification: C12M,C12N

Claim Text:
1. A method of delivering substance to target cells, comprising steps of: a) providing a system comprising an assembly including a plurality of elongate non-hollow nanoneedles forming a nanoneedle array for delivering the substance into the cells, at least some of the nanoneedles having a non-uniform diameter with a wider upper end, a narrower lower end for penetration into the cells and a length from substantially 200 nm to 100 um, wherein the lower end has a diameter from substantially 20-436 nm, wherein adjacent nanoneedles are spaced apart by substantially 5-50 um for minimizing multiple nanoneedles penetrating into one cell causing cell death, and wherein: b) allowing the substance be released or diffused from the nanoneedles into the cells intra-cellularly; c) applying the nanoneedle array to the cells under centrifugation at a speed from 300-1000 rpm (12.8-142 g); and d) allowing the substance to form a complex with a transfection reagent.