Patent ID: 9347959
Filing Date: 2016-05-24
CPC Classification: G01N

Claim Text:
1. A method for determine a risk of stroke, a transient ischemic attack (TIA) or peripheral artery disease (PAD) in a subject, the method comprising: a) obtaining a sample comprising plasma from a subject; b) determining the level of oxidized phospholipid (OxPL) on apolipoprotein B-100 (apoB) (OxPL/apoB) in the sample by contacting the OxPL and apoB with antibodies that specifically bind to the OxPL or apoB; c) determining the level of IgG and/or IgM autoantibodies to oxidation-specific epitopes (OSEs) comprising contacting the sample with a peptide mimotope selected from the group consisting of: NNWNMPL (SEQ ID NO:24); NNRNMPL (SEQ ID NO:25); NNYNMPL (SEQ ID NO:26); NNQNMPL (SEQ ID NO:27); NNWKMPL (SEQ ID NO:28); NNSHMPL (SEQ ID NO:29); KNSXQPL (SEQ ID NO:30); NNSXMPL (SEQ ID NO:31); QNSHMPL (SEQ ID NO:32); NNSNMPL (SEQ ID NO:35); NNSKMRL (SEQ ID NO:33); and DWAPHFT (SEQ ID NO:34), wherein the IgG and/or IgM autoantibodies bind to the peptide mimotope; and d) comparing the levels of IgG and/or IgM autoantibodies to OSEs in the sample to values of IgG and/or IgM autoantibodies indicative of high risk of stroke, TIA and/or PAD, and comparing the level of OxPL/apoB obtained from the sample with levels indicative of high risk of stroke, TIA and/or PAD, wherein if the level of OxPL/apoB and IgG and/or IgM autoantibodies in the sample falls within the levels OxPL/apoB and IgG and/or IgM autoantibodies of subject with high risk of stroke, TIA and/or PAD, the level in the sample of the subject is predictive for the risk of stroke, TIA and/or PAD in the subject.