Patent ID: 9447175
Filing Date: 2016-09-20
CPC Classification: A61K,A61P,C07K,Y02A

Claim Text:
1. A pharmaceutical composition comprising proteolytically processed, biologically active dichain neurotoxin, wherein the composition is free of detectable amounts of partially processed or unprocessed neurotoxin polypeptides, wherein the proteolytically processed, biologically active dichain neurotoxin is obtained by the steps of: a) contacting a solution comprising a mixture of processed and partially processed and/or unprocessed neurotoxin polypeptides with an antibody which has been raised against a peptide immunogen comprising the amino acid sequence of SEQ ID NO: 25; b) allowing the binding of the antibody to the unprocessed and/or partially processed neurotoxin polypeptides whereby complexes comprising the antibody and partially processed or unprocessed neurotoxin polypeptides are formed; and c) removing the complexes formed in step b) to obtain a solution of processed, active dichain neurotoxin, wherein the solution is free of detectable amounts of unprocessed and/or partially processed neurotoxin polypeptides; and d) formulating the solution of processed neurotoxin polypeptides obtained in step c) with at least one stabilizer of the biologically active dichain neurotoxin selected from protein stabilizers and non-protein stabilizers in pharmaceutically acceptable diluents, carriers, and/or adjuvants.