Patent ID: 9347960
Filing Date: 2016-05-24
CPC Classification: A61K,C07B,G01N

Claim Text:
1. A method for determining whether a human subject is at risk to develop one or more cardiovascular (CV) complications, wherein the method comprises: (a) determining in a biological sample obtained from the human subject the concentration of at least one ceramide of Formula I: wherein R (b) determining in the biological sample obtained from the human subject the concentration of at least one ceramide of Formula (II): wherein R (c) comparing the concentration of the at least one ceramide of Formula I and the concentration of the at least one ceramide of Formula II to a control sample; and (d) determining that the subject has an increased risk of developing one or more CV complications, if the biological sample contains a decreased concentration of the at least one ceramide of Formula I and an increased concentration of the at least one ceramide of Formula II, as compared to the control sample, wherein the method further comprises spiking the biological sample with at least one isotope-labelled ceramide of Formula I and at least one isotope-labelled ceramide of Formula II prior to determining the concentration of the at least one ceramide of Formula I and the at least one ceramide of Formula II and wherein the CV complications are selected from the group consisting of at least one of acute myocardial infarction, acute coronary syndrome, stroke and cardiovascular death.