Patent ID: 9399777
Filing Date: 2016-07-26
CPC Classification: C12N,C12Y

Claim Text:
1. A method for reducing the phenotypic expression of a nucleic acid of interest, which is normally capable of being expressed in a plant cell, said method comprising: providing unpolyadenylated RNA to the nucleus of the cell, wherein the unpolyadenylated RNA is produced by transcription of a chimeric DNA comprised within said cell, said chimeric DNA comprising a promoter operably linked to a target-specific DNA region encoding said RNA, and wherein the unpolyadenylated RNA comprises a target-specific sense nucleotide sequence, a target-specific antisense nucleotide sequence, and a spacer nucleotide sequence located between said sense and antisense nucleotide sequence, wherein the target-specific antisense nucleotide sequence comprises 20 consecutive nucleotides having 100% sequence identity to a sequence complementary to a sequence of 20 consecutive nucleotides of an untranslated region of an RNA molecule transcribed or produced from said nucleic acid of interest, and wherein the target-specific sense nucleotide sequence and the target-specific antisense nucleotide sequence form an artificial hairpin structure with each other.