Patent ID: 9429572
Filing Date: 2016-08-30
CPC Classification: C12M,G01N,G16B,G16C,Y10S

Claim Text:
1. A method for separating a complex comprising the following steps: (1) a step of arranging (a) a solution containing an analyte or an analogue thereof and (b) a solution containing not less than one kind of a substance capable of forming the complex with said analyte or said analogue thereof (the complex forming substance), each of (a) and (b) in a separate zone in a capillary, so that by applying a voltage to said capillary the complex between said analyte or said analogue thereof and the complex forming substance are formed without mixing these solutions in advance; (2) a step of forming the complex by electrophoretically concentrating said analyte or said analogue thereof and/or the complex forming substance by utilizing a difference in their electrophoretic mobility wherein said analyte or said analogue thereof and/or said complex forming substance are gathered on application of a voltage onto a capillary by applying a voltage to said capillary, before these solutions are uniformly mixed to form the complex between said analyte or said analogue thereof and the complex forming substance; and (3) a step of separating said complex, and the complex forming substance not involved in the formation of said complex or the analogue not involved in the formation of said complex by further electrical movement by using a different principle of electrophoresis from that in step (2), wherein step (2) is carried out by ITP (isotachophoresis) or FASS (Field Amplification Sample Stacking).