Patent ID: 9354228
Filing Date: 2016-05-31
CPC Classification: C07K,C12N,C40B,G01N,G16B,G16C

Claim Text:
1. A library comprising synthetic polynucleotides that encode an antibody heavy chain or alternative scaffold containing at least about 10 4 unique antibody CDRH3 amino acid sequences, wherein each of the polynucleotides encoding the at least about 10 4 unique antibody CDRH3 amino acid sequences has an antibody CDRH3 amino acid sequence represented by the following formula: [TN1]-[DH]-[N2]- [H3-JH], wherein: TN1 is a polypeptide corresponding to any of the TN1 polypeptides of Tables 10 and 18-26, or a polypeptide produced by translation of any of the TN1 polynucleotides of Tables 25-26; DH is a polypeptide corresponding to any of the DH polypeptides of Tables 11, 17-25 and 28, or a polypeptide produced by translation of any of the DH-encoding polynucleotides of Tables 16, 25 and 27; N2 is a polypeptide corresponding to any of the N2 polypeptides of Tables 12, 18-25 and 30, or a polypeptide produced by translation of any of the N2-encoding polynucleotides of Tables 25 and 29; H3-JH is a polypeptide corresponding to any of the H3-JH polypeptides of Tables 13, 15, 18-25 and 32, or a polypeptide produced by translation of any of the H3-JH-encoding polynucleotides of Tables 14, 25 and 31; wherein the diversity of the polynucleotides encoding the at least about 10 wherein the antibody heavy chain is a variable domain with framework (FRM) and complementary determining regions (CDR) comprising FRMH1-CDRH1-FRMH2-CDRH2-FRMH3-CDRH3-FRMH4; and wherein the alternative scaffold is selected from the group consisting of fibronectin, the β-sandwich, lipocalin, EETI-IFAGRP, BPTIFLAC1-D1/IT1-D2, thioredoxin, protein A, ankyrin repeats, γB-crystallin/ubiquitin, CTLD