Patent ID: 9375514
Filing Date: 2016-06-28
CPC Classification: A61K,A61L,A61P,C12N

Claim Text:
1. A method of producing a three-dimensional multicellular tissue or organ comprising: (a) culturing mammalian digests of acinary pancreatic tissue, acinary salivary gland tissue or exocrine sweat gland tissue in a medium to obtain acinary tissue cell aggregates or exocrine sweat gland tissue cell aggregates, (b) culturing the acinary tissue cell aggregates or exocrine sweat gland tissue cell aggregates obtained in step (a) in tissue vessels such that acinary tissue stem cells or exocrine sweat gland tissue stem cells adhere to said vessel, (c) cultivating the stem cells obtained from step (b) in medium in hanging drops, thereby forming organoid bodies comprising three-dimensional multicellular mammalian cell aggregates, wherein said organoid bodies contain cells of all three germ layers, and (d) cultivating the organoid bodies formed from step (c) on a physiologically compatible matrix coated tissue carrier in medium comprising differentiation factors and/or growth factors or in co-culture with differentiated cells or tissue pieces of the desired target tissue to obtain a desired tissue.