Patent ID: 9228168
Filing Date: 2016-01-05
CPC Classification: C12N

Claim Text:
1. A method for stabilizing a concentrated feed medium for feeding a mammalian cell culture comprising adding pyruvate to the feed medium, wherein the concentration of pyruvate after addition is at least 10 mM and not more than 100 mM, wherein the feed medium comprises cysteine at a concentration from 7 mM to 30 mM, wherein the feed medium comprises tyrosine at a concentration that is at least 1 mM and less than or equal to 4.6 mM, wherein the feed medium further comprises tryptophan at a concentration of 0.2-11 mM, histidine at a concentration of 0.6-67 mM, methionine at a concentration from 0.6-31 mM, phenylalanine at a concentration of 0.7-46 mM, isoleucine at a concentration of 1.6-80 mM, proline at a concentration of 2.6-130 mM, valine at a concentration of 2.5-128 mM, threonine at a concentration of 2.5-126 mM, serine at a concentration of 1.1-143 mM, lysine at a concentration of 2.4-165 mM, and leucine at a concentration of 2.2-115 mM, wherein cystine is not added to the feed medium at a concentration of more than 1.0 mM, wherein the pH of the feed medium is from 5.8 to about 7.4, and wherein the feed medium with the added pyruvate is stable for at least 1 week at 4-8° C.