Patent ID: 9459246
Filing Date: 2016-10-04
CPC Classification: G01N,G16B

Claim Text:
1. A method of evaluating the effects of a modulator on a plurality of discrete cell populations in communication, comprising (i) preparing a culture from a sample that has been removed from an individual, wherein the culture comprises a plurality of discrete cell populations derived from the sample in communication, and wherein the plurality of discrete cell populations comprises a first discrete cell population comprising monocyte cells and a second discrete cell population comprising a T-lymphocyte cells; (ii) contacting a first cell from the first discrete cell population in the culture with a modulator, wherein the modulator interacts with the first cell population but does not interact with the second discrete cell population in the culture, wherein the modulator comprises a toll-like receptor (TLR) activator; (iii) incubating the culture for a period of time; and (iv) after the incubation, determining an activation level of a first activatable element in single cells from the second cell population, wherein the activatable element is p-ERK or p-AKT.