Patent ID: 9458515
Filing Date: 2016-10-04
CPC Classification: C07H,C12Q

Claim Text:
1. A method for regulating the amount of protein produced from an RNA containing a 5′ cap structure and a coding region having an initiation codon at the 5′ end of the coding region and a stop codon at the 3′ end of the coding region, the RNA having a nucleoside compound introduced at a site selected from among the 5′ cap structure and 10 bases from the 5′ end of the RNA, wherein the nucleoside compound is such that a group is attached to (i) a carbon atom at position 8 of a purine nucleus or (ii) a carbon atom at position 5 or 6 of a pyrimidine nucleus, the group being represented by formula (I): where A represents an aryl group or a heteroaryl group, # represents a site where the group represented by the formula (I) is attached to the carbon atom at the position 8 of the purine nucleus or the carbon atom at the position 5 or 6 of the pyrimidine nucleus, and two Xs, which are identical to or different from each other, each represents a nitrogen atom or CH whose H may be substituted by alkyl, comprising the step of: