Patent ID: 9334532
Filing Date: 2016-05-10
CPC Classification: C12Q

Claim Text:
1. A method for the reduction of the complexity of nucleic acid pool(s), comprising: a) providing a sample with one or more nucleic acid molecules, b) cutting the nucleic acid molecules by a random and/or sequence independent cutting step thereby obtaining a pool of nucleic acid fragments, c) linking the pool of fragments to a common linker, d) providing a plurality of different amplification primer sets, wherein each amplification primer set is configured to selectively amplify linked fragments, wherein the different amplification primer sets differ in that they are configured to amplify a different nucleic acid feature outside of said common linker, wherein the amplification primer sets are provided separated from the other amplification primer sets, and e) selectively amplifying a fraction of said linked fragments with said plurality of different amplification primer sets to form a plurality of different subpools, with each subpool being the product of amplification by a separate different amplification primer set, amplification products of each subpool sharing the different nucleic acid feature, wherein at least one subpool comprises more than one different fragment.