Patent ID: 9528146
Filing Date: 2016-12-27
CPC Classification: B01J,C07B,C07H,C12N,C12Q,C40B,G01N

Claim Text:
1. A process for detecting or quantifying more than one nucleic acid of interest in a library comprising the steps of: a) providing: b) contacting said library of nucleic acid analytes with said first set of primers to form more than one first bound entity; c) extending said bound first set of primers by means of template sequences provided by said nucleic acid analytes to form first copies of said analytes; d) contacting said extended first copies with said second set of primers to form more than one second bound entity; e) extending said bound second set of primers by means of template sequences provided by said extended first copies to form an extended second set of primers; f) separating said extended second set of primers obtained in step e); g) contacting said extended second set of primers with said third set of primers to form more than one third bound entity; h) extending said third bound entity by means of template sequences provided by said extended second set of primers to form more than one complex comprising said extended third bound entity and said extended set of primers; i) synthesizing from a production center in said second set of primers in said complexes one or more nucleic acid copies under isothermal or isostatic conditions; j) hybridizing said nucleic acid copies formed in step i) to said array of nucleic acids provided in step a) (i); and k) detecting or quantifying any of said hybridized copies obtained in step j).