Patent ID: 9267943
Filing Date: 2016-02-23
CPC Classification: B03C,G01N

Claim Text:
1. A device for capture and isolation of target cells from a mixed cell population wherein the target cells are labeled with magnetically responsive material to form labeled target cells, comprising: (a) a container for holding the labeled target cells; (b) a magnetic member having a tip end and another end connected to an actuator; (c) a sleeve between the magnetic member and the mixed cell population to prevent direct contact of cells with the magnetic member, said sleeve being nonmagnetic and nonadherent to cells in the mixed cell population; (d) said sleeve being separable from the magnetic member by the actuator, which actuator causes movement between the magnetic member and the sleeve, whereby the magnetic member is moved more or less into the sleeve; and (e) said actuator being constructed and arranged for causing in the device (i) relative orbital stirring movement between the magnetic member and the mixed cell population to contact the magnetic member with a majority of cells in the mixed cell population, and (ii) movement of the magnetic member into and out of the container to retrieve target cells from the mixed cell population.