Patent ID: 9488652
Filing Date: 2016-11-08
CPC Classification: C12N,G01N

Claim Text:
1. A method for solid phase isolation, enrichment and/or detection of tumor stem cells and/or tumor cells in epithelial-mesenchymal transition from a sample of body fluid containing such cells comprising (a) contacting the sample with a solid surface that preferentially binds the cells, and (b) then washing the solid surface, wherein the sample contains a polyol at least during step (a) or step (b), and in an optional detection step, detecting isolated or enriched cells by the expression of at least one marker associated with at least one of the group comprising tumor stern cells and tumor cells in epithelial-mesenchymal transition, wherein the marker is selected from the group consisting of TWIST, ALDH1, BMI1, Akt2, PI3KCA and SIP1.