Patent ID: 9469872
Filing Date: 2016-10-18
CPC Classification: C09K,C12P,C12Q

Claim Text:
1. A method for amplifying nucleic acid templates of different sequences comprising (a) a first round comprising one or more cycles of amplification, wherein the cycles of amplification comprise amplifying nucleic acid templates in an ensemble under conditions favoring copying AT rich templates over GC rich templates such that the efficiency of amplification of AT rich templates is not reduced or inhibited relative to non-AT rich templates; and (b) a second round comprising one or more cycles of amplification, wherein the cycles of amplification comprise amplifying the nucleic acid templates in the ensemble under conditions favoring copying GC rich templates over AT rich templates such that the efficiency of amplification of AT rich templates is not reduced or inhibited relative to non-AT rich templates; wherein the nucleic acid templates are immobilized on a solid support, and wherein steps (a) and (b) are repeated one or more times.