Patent ID: 9463461
Filing Date: 2016-10-11
CPC Classification: B01L,G01N

Claim Text:
1. A sample processing cartridge, comprising: an upper cartridge portion comprising a first substrate and a first hydrophobic layer formed on a surface of the first substrate; a lower cartridge portion comprising a second substrate and a second hydrophobic layer formed on a surface of the second substrate; a spacer frame formed between the upper portion and the lower portion; a sample inlet section comprising a sample inlet; a sample analysis section offset by a distance from the sample inlet section; a channel that (a) is at least partially defined by sidewalls of the spacer frame, (b) is at least partially filled with an isolation medium, and (c) fluidically couples the sample inlet section and the sample analysis section; at least one magnetic bead disposed in the channel; a plurality of first openings that extend through the first hydrophobic layer and expose a corresponding plurality of hydrophilic surfaces of the first substrate; at least one second opening that extends through the second hydrophobic layer and exposes a corresponding hydrophilic surface of the second substrate; at least one aqueous reagent pendant disposed in the channel and isolated by the isolation medium, wherein a portion of the at least one aqueous reagent pendant contacts one of the plurality of exposed hydrophilic surfaces of the first substrate; and at least one biochemical reaction mixture disposed in the analysis section and isolated by the isolation medium, wherein a portion of the at least one biochemical reaction mixture contacts another one of the plurality of exposed hydrophilic surfaces of the first substrate, and another portion of the at least one biochemical reaction mixture contacts the exposed hydrophilic surface of the second substrate, wherein the isolation medium is permeable to the at least one magnetic bead but not to the at least one aqueous reagent pendant.