Patent ID: 9360457
Filing Date: 2016-06-07
CPC Classification: C12Q,G01N,G01R,Y10T

Claim Text:
1. A method for amplifying a target fungal nucleic acid in a whole blood sample, the method comprising: (a) contacting a whole blood sample suspected of containing one or more fungal cells with an erythrocyte lysis agent, thereby lysing red blood cells; (b) centrifuging the product of step (a) to form a supernatant and a pellet; (c) discarding some or all of the supernatant of step (b) and resuspending the pellet to form an extract; (d) combining said extract of step (c) with beads to form a mixture and agitating the mixture to form a lysate, said lysate containing both subject cell nucleic acid and fungal nucleic acid; and (e) providing the lysate of step (d) in a detection tube and amplifying fungal nucleic acids therein by PCR to form an amplified lysate solution; wherein ten fungal cells per milliliter of said whole blood sample is sufficient to permit amplification of said target fungal nucleic acid.