Patent ID: 9469867
Filing Date: 2016-10-18
CPC Classification: C07H,C12Q

Claim Text:
1. A process of detecting the presence or absence of a target nucleic acid, comprising the steps of: (a) contacting an oligonucleotide probe to a target nucleic acid, thereby forming a complex between the probe and the target nucleic acid, wherein the probe comprises a dR-O—[C]n nucleotide that lacks a base and has a sugar with a carbon at a 1′ position, and wherein the carbon at the 1′ position is covalently linked through an oxygen atom to a carbon atom of a linker containing n carbon atoms wherein n is 1-8; (b) contacting the probe/nucleic acid complex with a nuclease selected from the group consisting of AP endonuclease, DNA glycosylase/lyase and DNA glycosylase, wherein the nuclease can excise the linker from the complex and/or specifically cleave the probe at the dR-O—[C]n nucleotide; and (c) detecting whether such excision or cleavage has occurred.