Patent ID: 9528143
Filing Date: 2016-12-27
CPC Classification: C12Q

Claim Text:
1. An aqueous reagent for an ex-vivo polymerase reaction, the reagent comprising Taq DNA polymerase, an anionic tracer dye selected from the group consisting of acid violet 5, acid red 1, acid red 106, acid red 4, a salt of any one thereof, and combinations of acid violet 5 and acid red 1, the anionic tracer dye being unbound to primer or nucleotides, and a solute to increase the physical density of the reagent, wherein the anionic tracer is present in the reagent in an amount sufficient to be visible for a post-polymerase reaction analysis, the reagent being free of primers and nucleic acid polymer template and having a physical density of at least 1.01 g/cm 3 , but less than the density of the solute.