Patent ID: 9274123
Filing Date: 2016-03-01
CPC Classification: C07D,G01N,Y10T

Claim Text:
1. A method for analyzing a compound having an amino group, an imino group, or mixture thereof, in a sample by tandem mass spectrometry, comprising: (a) labeling the compound having an amino group, an imino group, or mixture thereof with a carbamate compound represented by formula (Ia) or a salt thereof to form a labelled compound having a bond between a nitrogen of the amino group, imino group, or mixture thereof and a labeling a portion of the carbamate compound represented by formula (Ia) or a salt thereof: wherein Ar is an aromatic carbocyclic group or an aromatic heterocyclic group, wherein the aromatic carbocyclic group or the aromatic heterocyclic group may have one or more substituents, (b) selecting or scanning, in a first mass analyzer in positive ion mode, m/z values derived from pre-selected ions of the labeled compound or ranges of m/z values which include ions of the labeled compounds; (c) collisionally cleaving the ions of the labeled compound at the bond between the nitrogen of the amino group or the imino group of the labelled compound and the labeling portion of the carbamate compound represented by formula (Ia) or a salt thereof to produce a fragment ion and a neutral fragment; and (d) selecting or scanning, in a second mass analyzer: (1) pre-selected m/z values of a fragment ion derived from the labeling portion of the carbamate compound represented by formula (Ia) or a salt thereof, (2) pre-selected m/z values of a fragment ion derived from the compound having an amino group, an imino group, or a mixture thereof, or (3) ranges of m/z values at a pre-selected offset value from the first mass analyzer, wherein the offset is derived from loss of the labeling portion of the carbamate compound represented by formula (Ia) or a salt thereof.