Patent ID: 9493818
Filing Date: 2016-11-15
CPC Classification: C12Q

Claim Text:
1. A method of detecting the presence of one or more target RNAs in a sample, said method comprising: a) ligating an adapter or linker oligonucleotide to an end of said one or more target RNAs to produce one or more extended target polynucleotides; b) circularizing said one or more extended target polynucleotides by ligating the 5′-end of the one or more extended target polynucleotides to its 3′-end; c) synthesizing, by rolling circle amplification, multimeric nucleic acids (MNAs) comprising multiple repeats of sequences that are complementary to said one or more circularized extended polynucleotides; and d) sequencing said MNAs or portions thereof.