Patent ID: 9518304
Filing Date: 2016-12-13
CPC Classification: C12N,C12P,C12R,Y02E

Claim Text:
1. A method for generating a nicotine resistant microorganism, the method comprising: (a) culturing a microorganism in a medium conducive to growth, wherein the medium comprises a first concentration of nicotine that retards but does not inhibit growth of the microorganism; (b) selecting a single colony of the microorganism and inoculating the single colony into a fresh culture of the medium conducive to growth, wherein the fresh medium comprises a second concentration of nicotine that is incrementally higher than the first concentration and that retards but does not inhibit growth of the microorganism; and (c) repeating step (b) until the desired concentration of nicotine is achieved or until growth of the microorganism is completely inhibited, wherein the microorganism produced is resistant to the desired concentration of nicotine.