Patent ID: 9234185
Filing Date: 2016-01-12
CPC Classification: A61K,A61P,C12N

Claim Text:
1. A method for isolating a chimeric adenovirus comprising an E2B region, wherein said method comprises: a) pooling of adenoviral serotypes independently selected from the group consisting of adenoviral subgroups B-F, thereby creating a pooled adenoviral mixture; b) passaging the pooled adenoviral mixture from step (a) on an actively growing culture of tumor cells at a particle cell ratio high enough to encourage recombination between serotypes but not so high as to produce premature cell death; c) harvesting supernatant from step (b); d) infecting a quiescent culture of tumor cells with the supernatant harvested in step (c); e) harvesting cell culture supernatant from step (d) prior to any sign of cytopathic effect; f) infecting a quiescent culture of tumor cells with the cell culture supernatant harvested in step (e); and g) isolating from the supernatant in step (f) by plaque purification a chimeric adenovirus having a nucleic sequence comprised of nucleic acid sequences of at least two adenovirus serotypes, wherein said chimeric virus is oncolytic.