Patent ID: 9365827
Filing Date: 2016-06-14
CPC Classification: A61K,A61P,C07H,C07K,C12N

Claim Text:
1. A method for producing human cardiomyocytes comprising the steps of: a) culturing a selected human pluripotent stem cell clone having been prepared from a single pluripotent stem cell, the human stem cell clone being either a human embryonic stem cell clone or a human induced pluripotent stem cell clone; b) individualizing the cultured human pluripotent stem cells of step (a); c) culturing the individualized human pluripotent stem cells obtained from step (b) under defined conditions in suspension culture in an aggregate promotion media comprising fibroblast growth factor 2 (FGF2), and a Rho-associated protein kinase (ROCK) inhibitor or a myosin II inhibitor, to form cell aggregates; d) culturing said cell aggregates in cardiac differentiation medium comprising FGF2, Activin, and bone morphogenetic protein 4 (BMP4) to form cardiomyocytes; and e) obtaining the cardiomyocytes.