Patent ID: 9388407
Filing Date: 2016-07-12
CPC Classification: C12M,C12N,C12P

Claim Text:
1. A method for synthesizing a target polynucleotide having a predetermined sequence, the method comprising (a) providing a solid support having a first feature and a second feature thereon, the first feature having a first plurality of immobilized oligonucleotides each comprising a first predetermined subunit sequence and a first degenerate binding sequence, the second feature having a second plurality of immobilized oligonucleotides each comprising a second predetermined subunit sequence and a second degenerate binding sequence, wherein the first predetermined subunit sequence and the second predetermined subunit sequence together comprise a target polynucleotide; (b) generating, in a chain extension reaction using an input oligonucleotide as primer that anneals with the first degenerate binding sequence, a first product comprising the first predetermined subunit sequence at the first feature; (c) advancing, via microfluidic communication, the first product to the second feature; and (d) generating, in a chain extension reaction using the first product as primer that anneals with the second degenerate binding sequence, a second product comprising the first and the second predetermined subunit sequences at the second feature.