Patent ID: 9284521
Filing Date: 2016-03-15
CPC Classification: B01D,B01F,C07K,C12M,C12P

Claim Text:
1. A method for producing a biological product comprising: a) providing a bioreactor including: b) disposing the flexible container in the cylindrical support and immovably fixing the flexible container to the inner surface of the cylindrical support; c) introducing the nutrient media in the flexible container through the liquid inlet; d) introducing a biological culture capable of growing in the nutrient media and producing a biological product in the flexible container through the liquid inlet; e) heating or cooling the nutrient media to a pre-determined temperature; f) connecting the gas inlet to a source of a compressed gas; g) starting the flow of the compressed gas with sufficient pressure to achieve a pre-determined pressure in the flexible container that causes the flexible container to expand and stay in continuous contact with the inner surface of the cylindrical support; h) continuing the flow of compressed gas to maintain the pre-determined pressure in the flexible container; i) moving the shaft to pivot the cylindrical support between the angles from −110 and +110 degrees at a pre-determined frequency; j) monitoring the density and the viability of the biological culture in the nutrient media at predetermined time intervals; k) monitoring the concentration of the biological product in the nutrient media at predetermined time intervals; l) stopping the pivoting of the cylindrical support when the density of the biological culture or the concentration of the biological product reaches a pre-determined level and removing the nutrient media from the flexible container for further processing of the purification of the biological product in the nutrient media.