Patent ID: 9354240
Filing Date: 2016-05-31
CPC Classification: C07K,C12N,G01N

Claim Text:
1. A method for separating cells or cell populations from a biological tissue, comprising: applying two or more probes respectively containing biological-component binding domains of two or more proteases and two or more visualization molecules to an isolated biological tissue, measuring binding amounts of the probes to the biological tissue through the visualization molecules, determining a quantitative ratio of the proteases based on results of the measurement, applying the proteases in the quantitative ratio to the biological tissue, and separating the cells or the cell populations from the biological tissue, wherein each of the proteases is selected from the group consisting of collagenase, trypsin, chymotrypsin, dispase, elastase, papain, pronase, thermolysin, subtilisin, bromelain, phytin, and thermitase, and wherein each of the two or more visualization molecules is a molecule that is different from the other two or more visualization molecules and that is selected from the group consisting of fluorescent molecules, luminescent molecules, and radioisotopes including a positron nuclide.