Patent ID: 9334542
Filing Date: 2016-05-10
CPC Classification: C12Q

Claim Text:
1. A method for differentially detecting a non- Mycobacterium avium subsp. paratuberculosis (non-MAP) Mycobacterium Avium Complex (MAC) organism selected from the group consisting of Mycobacterium avium subsp. Avium (MAA), Mycobacterium avium subsp silvaticum (MAS), and Mycobacterium subsp hominissuis (MAH), from a Mycobacterium avium subsp. paratuberculosis (MAP) organism comprising: amplifying from a sample at least one non-MAP-specific target nucleic acid selected from the group consisting of SEQ ID NO:1, SEQ ID. NO: 2, SEQ ID NO: 3, SEQ ID NO: 4, SEQ ID NO: 5, SEQ ID NO: 6, SEQ ID NO: 7, SEQ ID NO: 8, and full complements thereof, comprising contacting nucleic acids from the sample with at least one primer set that can hybridize to and amplify the at least one non-MAP-specific target nucleic acid, under conditions suitable for amplification; and detecting an amplified nucleic acid, wherein detecting the amplified nucleic acid is indicative of the presence of a non-MAP MAC organism in the sample.