Patent ID: 9357755
Filing Date: 2016-06-07
CPC Classification: A01K,C12N

Claim Text:
1. A transgenic silk worm comprising a plurality of recombinant nucleic acids encoding mammalian glycosylation enzymes and optionally a nucleic acid encoding a heterologous protein of interest to be glycosylated, said silkworm having a genome in which is integrated, in one or more copies: a) first construct being pBSII-A3/EYFP-P25/Gal/GnT encoding both a beta 1,4-galactosyltransferase (Gal) and a beta-1,2-N-acetylglucosaminyltransferase II (GnT); b) a second construct being pBSII-3xP3/ECFP-P25/ST6/ST3 encoding both alpha-2,6-sialyltransferase (ST6) and alpha 2,3-sialyltransferase (ST3); and c) a third construct being pBSII-3xP3/DsRed2-P25/CMP/SAS encoding both a sialic acid synthetase (SAS) and a CMP sialic acid synthetase (CMP), wherein each recombinant nucleic acid encoding a glycosylation enzyme is operably linked to an expression control sequence, and wherein expression of said glycosylation enzymes in said transgenic silk worm allows for production of a partially or completely mammalianized glycosylated protein in the silk worm.