Patent ID: 9434931
Filing Date: 2016-09-06
CPC Classification: A61P,C12N,C12Y

Claim Text:
1. A recombinant single-chain meganuclease comprising: (a) a first subunit comprising a polypeptide having at least 85% sequence identity to residues 9-151 of SEQ ID NO: 1; (b) a second subunit comprising a polypeptide having at least 85% sequence identity to residues 9-151 of SEQ ID NO: 1; and (c) a polypeptide linker comprising 28-45 amino acids, said polypeptide linker being covalently bound to said first and said second subunit; said first subunit being covalently bound to said polypeptide linker at a residue corresponding to a position selected from the group of positions 153-163 of SEQ ID NO: 1; said second subunit being covalently bound to said polypeptide linker at a residue corresponding to a position selected from the group of positions 1-9 of SEQ ID NO: 1; wherein said polypeptide linker comprises an ALRAGA (SEQ ID NO:75) sequence; and wherein said first and second subunits are capable of functioning together to recognize and cleave a non-palindromic DNA sequence.