Patent ID: 9439898
Filing Date: 2016-09-13
CPC Classification: A61K,C12Q,G01N

Claim Text:
1. A method for examining cytotoxic potency of a compound or chemical agent for selectively inducing cytotoxicity in Gefitinib-resistant non-small-cell lung cancer harboring T790M mutation comprising providing Gefitinib-resistant non-small-cell lung cancer cells harboring said mutation or transfecting into normal cells with an expression vector containing a construct of epidermal growth factor receptor having said mutation; applying an effective amount of said compound or chemical agent to either said cancer cells or normal cells transfected with said expression vector, wherein the cytotoxic potency of said compound or chemical agent is indicated by an elevating level of reaction oxygen species through up-regulation and activation of NOX3 which triggers degradation of epidermal growth factor receptor and leads to apoptosis of said cancer cells.