Patent ID: 9244012
Filing Date: 2016-01-26
CPC Classification: B05D,C09D,C23C,G01N,Y10T

Claim Text:
1. A method of fabricating a metallized substrate that includes multiple layers of metallic nanoparticles that exhibit increased metal enhanced fluorescence, the method comprising: a) providing a substrate; b) providing a metal containing solution, wherein the metal has plasmonic activity, contacting the metal containing solution with the substrate and heating same to a temperature of from about 30° C. to about 60° C. for a deposition time period ranging from 1 minute to 7 minutes to provide for deposition of metallic nanoparticles; c) transferring the heated substrate to a freezing chamber for a time period ranging from about 1 minute to 4 minutes to form a chilled substrate; d) transferring the chilled substrate back to a heating environment for a time period as in step b) or shorter than the previous heating period and in the same heating temperature range; e) removing the solution; and f) repeating steps b) and c) for at least one more time and optionally from 4 to 19 times to provide a multiplicity of layers of metallic nanoparticles, wherein the nanoparticles are discrete and substantially the same size.