Patent ID: 9469871
Filing Date: 2016-10-18
CPC Classification: B01L,C12Q

Claim Text:
1. A method for amplification and detection of a target nucleic acid sequence in a sample, the method comprising: transferring the sample through an inlet into a diagnostic apparatus; distributing the sample among a plurality of microfluidic channels of the diagnostic apparatus, each microfluidic channel extending to one of a plurality of reaction chambers in the diagnostic apparatus, at least one of the reaction chambers having a nucleic acid amplification reagent therein, so that a portion of the sample is received in each reaction chamber; heating the reaction chambers in order to amplify any target nucleic acid sequence within the sample portion; manually sliding a locking valve within a void of the diagnostic apparatus from an open position to a closed position after the transferring step to block all of the microfluidic channels and prevent backflow of the sample portion from each reaction chamber through the microfluidic channel extending to it during heating; and detecting in the at least one reaction chamber amplification of the target nucleic acid sequence within the sample.