Patent ID: 9475046
Filing Date: 2016-10-25
CPC Classification: B01L,G01N

Claim Text:
1. A method for determining the concentration of a biologically active substance in a sample by the means of a radiation emitting enzyme-linked immunosorbent assay (ELISA) performed on a solid phase within a tubing, the tubing surface forming the solid support and the tubing surface within the reaction cell forming an active surface continuously along the entire reaction cell, by detecting and quantifying the radiation emitted within the tubing, the radiation being proportional to the amount of the biological active substance assayed, by the means of a detector, comprising the steps of introducing ELISA related fluids and the sample into a microchip substantially extending in one plane, comprising the tubing, wherein said tubing encompasses a plurality of curves and wherein every other curve of the tubing is substantially a 180° curve to the right and the remaining intervening curves are substantially 180° curves to the left wherein the distance between each curve of the tubing is increasing in a direction towards a center position of the microchip and is decreasing in a direction away from the center position of the microchip to form a reaction cell, conducting the fluids through said reaction cell formed by said tubing, in which reaction cell the radiation emitting activity takes place, and detecting the light emitted from the reaction cell substantially perpendicular to the plane in which the curves extend, wherein detection of the light emitted from the reaction cell is proportional to the concentration of the biologically active substance.