Patent ID: 9321993
Filing Date: 2016-04-26
CPC Classification: A61K,C12N

Claim Text:
1. A method for producing cartilage tissue, comprising: (a) introducing chondrogenic progenitor cells into a scaffold comprising a matrix material; (b)(i) contacting the chondrogenic progenitor cells with chondroitinase (cABC) and culturing the chondrogenic progenitor cells in a culture medium comprising trimethylamine N-oxide (TMAO) or (ii) culturing the chondrogenic progenitor cells in a culture medium comprising trimethylamine N-oxide (TMAO) and cABC; (c) differentiating the cultured chondrogenic progenitor cells into connective tissue cells; (d) digesting the cultured chondrogenic progenitor cells or connective tissue cells with the cABC; and (e) forming cartilage tissue from the connective tissue cells, the cartilage tissue comprising an extracellular matrix, the extracellular matrix comprising collagen fibers, proteoglycans, and elastin fibers; wherein,