Patent ID: 9388467
Filing Date: 2016-07-12
CPC Classification: B01J,B01L,C12Q,G16B

Claim Text:
1. A quantitative method for a target DNA contained in a biological sample using a biochip, comprising: introducing a sample into a type I chamber and a type II chamber of the biochip; performing a PCR amplification in the type I chamber and the type II chamber; measuring a fluorescence intensity of the fluorescent probe in the type I chamber and the type II chamber; determining a regression curve of equation (1) below based on the measured fluorescence intensities in the type I chamber and the type II chamber, and amounts of the internal standard DNA contained in the type I chamber and the type II chamber, where F is a fluorescence intensity, C is an amount of internal standard DNA, b is an amount of target DNA contained in the sample, and a and c are predetermined values; and estimating the amount of the target DNA contained in the sample based on the regression curve, wherein the biochip includes a supply reservoir, a waste chamber, the type I chamber, the type II chamber, and a plurality of pathways connecting the supply reservoir, the waste chamber, the type I chamber, and the type II chamber, and the pathways are configured such that when the biochip, in a first orientation, is rotated the plurality of pathways prevent flow of a mixture of the primer, the internal standard DNA, and fluorescent probe to the waste chamber, and the pathways are configured such that when the biochip, in a second orientation, is rotated the plurality of pathways allow flow of the mixture to the waste chamber.