Patent ID: 9416358
Filing Date: 2016-08-16
CPC Classification: C07H,C12N,C12Q

Claim Text:
1. A method for producing a single-molecule circular DNA that is not derived from multiple-molecule circular DNA, the method comprising the following steps: A) a step of producing circular DNA molecules by the method of steps 1-6: B) a step of determining the sequence of an adapter (b) portion in the produced circular DNA molecules and selecting only single-molecule circular DNA that is not derived from multiple-molecule circular DNA, wherein the circular DNA molecule is determined to be single-molecule circular DNA that is not derived from multiple-molecule circular DNA, when two unique sequences contained in the adapter (b) portion are identical to each other, and the circular DNA molecule is determined to be multiple-molecule circular DNA or single-molecule circular DNA derived from the multiple-molecule circular DNA, when the two unique sequences are different from each other.