Patent ID: 9529003
Filing Date: 2016-12-27
CPC Classification: C07K,G01N,Y10T

Claim Text:
1. A composition comprising (a) a probe that binds to bilirubin but does not bind significantly to fatty acid comprising an intracellular lipid binding protein (iLBP) mutein labeled with a first fluorophore, wherein the iLBP mutein corresponds to SEQ ID NO: 3 comprising one or more amino acid substitutions at positions selected from the group consisting of positions 14, 18, 23, 25, 27, 31, 36, 38, 55, 60, 72, 73, 74, 78, 102, 104, 106, 115 and 117, and wherein the first fluorophore is attached to a lysine residue, the N-terminus amino group of the iLBP, or to a cysteine substitution, and (b) a second fluorophore, wherein the second fluorophore is free in solution or attached to a protein which does not bind bilirubin, and wherein the first fluorophore and the second fluorophore are capable of excitation at the same wavelength, and wherein the emission wavelength of the first fluorophore and the second fluorophore are different, and wherein the second fluorophore does not change its emission in response to bilirubin binding to the iLBP mutein.