Patent ID: 9234189
Filing Date: 2016-01-12
CPC Classification: C12N

Claim Text:
1. A method for cloning a hybrid-type histidine kinase gene into a recombinant plasmid, comprising the steps of: (i) isolating cDNA from rice cv IR64; (ii) providing a polymerase chain reaction mixture comprising: (iii) performing a polymerase chain reaction (PCR) using the mixture of step (ii) to produce an amplified hybrid-type histidine kinase (OsHK3b) gene consisting of SEQ ID NO: 3 of the rice cv IR64 cDNA; and (iv) cloning the amplified (OsHK3b) gene of step (iii) into the recombinant plasmid, wherein in said PCR, the step of annealing is performed at about 55° C.