Patent ID: 9295965
Filing Date: 2016-03-29
CPC Classification: B01J,C12N,C12P

Claim Text:
1. A method for producing at least one target polynucleotide having a predefined sequence, the method comprising: a. providing at least a first and a second pluralities of support-bound, single-stranded, template oligonucleotides, wherein each of the first and second pluralities of template oligonucleotides has a predefined sequence and is bound to a first and second feature of one or more planar support, respectively, each of the first plurality of template oligonucleotides comprising a sequence region at its 5′ end complementary to a sequence region of a 5′ end of the second plurality of template oligonucleotides; b. generating at least a first and a second pluralities of construction oligonucleotides complementary to the first and second pluralities of template oligonucleotides in a chain extension reaction, each of the first plurality of construction oligonucleotides comprising a sequence region at its 3′ end that is complementary to a sequence region of a 3′ end of the second plurality of construction oligonucleotides; c. providing a plurality of support-bound, single-stranded, anchor oligonucleotides at a selected feature on a planar anchor support, wherein the 5′ end of each anchor oligonucleotide is complementary to the 5′ end of the first plurality of construction oligonucleotides; and d. transferring the first plurality of construction oligonucleotides from the first feature to the selected feature on the planar anchor support, transferring the second plurality of construction oligonucleotides from the second feature to the selected feature on the planar anchor support, and hybridizing the at least first and second pluralities of construction oligonucleotides to the plurality of anchor oligonucleotides on the planar anchor support.