Patent ID: 9334529
Filing Date: 2016-05-10
CPC Classification: C12Q

Claim Text:
1. A method for genotyping N loci in a target nucleic acid molecule present in a sample, wherein N is at least 1 and each locus is located in a respective genotype marker region of the nucleic acid molecule, and corresponds to two or more genotypes, the method comprising the steps of: a) performing a pre-amplification of each genotype marker region utilizing primer-dependent enzymatic reactions, wherein the amplification is performed with a first group of amplification primer pairs consisting of a first set of one or more different forward primers and a first set of one or more different reverse primers, yielding one or more amplicons encompassing the genotype marker region(s), and wherein each amplicon contains a region with at least one nucleotide sequence that can act as a primer binding target distinct from the genotype marker region; b) performing on the amplicon(s) from step a) a two-level nucleic acid amplification reaction utilizing primer-dependent enzymatic reactions, wherein c) genotyping said N loci of the target nucleic acid molecule by