Patent ID: 9353379
Filing Date: 2016-05-31
CPC Classification: C12N,Y02A

Claim Text:
1. A method for hydroponic cultivation of a genetically-modified plant comprising: cultivating the genetically-modified plant in a hydroponic medium, wherein the genetically-modified plant is transformed by introducing an expression vector comprising: a promoter that regulates expression of RNA expressed in a seed that satisfies the formula (1): wherein V and W are determined as follows: V is an amount of RNA in the seed of a predetermined plant when the predetermined plant is cultivated in a hydroponic medium adjusted so that nitrate nitrogen is 70 mg/L to 750 mg/L and/or ammonium nitrogen is 70 mg/L to 750 mg/L for a period which starts from 30 days before the expected flowering date and ends at the flowering day, and W is an amount of RNA contained in a seed of the predetermined plant when the plant is cultivated in a medium adjusted so that nitrogen is 0 mg/L to 50 mg/L for a period which starts from 30 days before the expected flowering date and ends at the flowering day, wherein the predetermined plant is the same species as the genetically-modified plant, wherein RNA is extracted from the seeds 15 to 25 days after the flowering, wherein the promoter is a glutelin promoter; and a polynucleotide located downstream of the promoter and encoding a protein of interest, wherein the medium for cultivating the genetically-modified plant is adjusted so that a content of nitrate nitrogen is from 70 mg/L to 750 mg/L and a content of ammonium nitrogen is from 50 mg/L to 750 mg/L for a period which starts from 30 days before the expected flowering date and ends at the flowering day of the genetically-modified plant, and wherein the ratio of the contents of the nitrate nitrogen to the ammonium nitrogen is from 3:1 to 1:3.