Patent ID: 9365832
Filing Date: 2016-06-14
CPC Classification: A61K,B01D,C12N

Claim Text:
1. A method for the purification of herpes simplex virus (HSV) particles from a mammalian host cell culture comprising the steps of: a) treating the mammalian host cell culture with a viral releasing agent to release the HSV particles from the host cells; b) treating the material from step (a) with an endonuclease; c) filtering the material from step (b) to remove any intact cells or cellular debris; d) applying the solution obtained from step (c) to an anion exchange chromatography resin; e) eluting the HSV particles from the anion exchange column; f) subjecting the eluent from step (e) to tangential flow filtration; and g) recovering the purified HSV particles.