Patent ID: 9494588
Filing Date: 2016-11-15
CPC Classification: A61K,C07K,G01N

Claim Text:
1. A protein produced by an expression system comprising a nucleic acid sequence that encodes an MHC class I heavy chain, wherein, the MHC class I heavy chain comprises an alpha-1 helix and an alpha-2 helix, the nucleic acid sequence has a disulfide bridge formed between the alpha-1 helix and the alpha-2 helix in the MHC class I heavy chain, the amino acid 139 is substituted by a cysteine so as to provide Cys-139, the amino acid 84 is substituted by the cysteine so as to provide Cys-84 or the amino acid 85 is substituted by the cysteine so as to provide Cys-85, the disulfide bridge is formed between the alpha-1 helix and the alpha-2 helix in the MHC class I heavy chain between Cys-139 and Cys-84 or between Cys-139 and Cys-85, and the protein comprises an anchor element selected from a natural biotinylation sequence, a polyhistidine sequence, or a polyarginine sequence.