Patent ID: 9291596
Filing Date: 2016-03-22
CPC Classification: G01N

Claim Text:
1. An electroblot transfer method comprising assembling a membrane and a gel, the gel containing at least one of separated proteins, peptides, or nucleic acids, the membrane and the gel sandwiched between at least two pieces of absorbent material, the absorbent material saturated with a transfer buffer providing a source of ions and comprising N-(2-hydroxy-1,1-bis(hydroxymethyl)ethyl)glycine (tricine), tris(hydroxymethyl)aminomethane (tris), glycine, and optionally ethylenediaminetetraacetic acid (EDTA); and applying a current to effect reduced resistance in transfer of at least one of the separated proteins, polypeptides, or nucleic acids from the gel to the membrane, wherein said ions of said saturated absorbent material drive said transfer.