Patent ID: 9493616
Filing Date: 2016-11-15
CPC Classification: A61K,A61P,C07K,C08H

Claim Text:
1. A method for preparing polyethylene glycol conjugated hemoglobin (PEG-Hb) comprising the steps of: a) mixing hemoglobin (Hb) with 2-iminothiolane (2-IT) in an aqueous diluent, wherein the 2-IT is at a concentration of between 7- and 8-fold molar excess in the diluent over the Hb concentration, to form thiolated Hb; and b) adding polyethylene glycol (PEG)-maleimide (Mal) to the thiolated Hb in the aqueous diluent, wherein the PEG-Mal is at a concentration of between 9- and 15-fold molar excess in the diluent over the Hb concentration to form a PEG-Hb conjugate, wherein the PEG-Mal has an average molecular weight of between 4,000 and 6,000 daltons (Da); wherein the PEG-Hb conjugate contains an average of between 7.1 and 8.9 PEG molecules per Hb.