Patent ID: 9470687
Filing Date: 2016-10-18
CPC Classification: G01N

Claim Text:
1. A method for determining whether the cancer in a patient suffering from non-small cell lung cancer is adenocarcinoma or squamous cell carcinoma comprising measuring the level of the KRT5, KRT7, Napsin A, TTF1, TP63, and MUC1 proteins in a human biological sample of formalin-fixed tumor tissue from said patient, comprising detecting and quantifying the amount of a KRT5 fragment peptide, a KRT7 fragment peptide, a Napsin A fragment peptide, a TTF1 fragment peptide, a TP63 fragment peptide, and a MUC1 protein fragment peptide in a protein digest prepared from said biological sample, using mass spectrometry; and calculating the level of KRT5, KRT7, Napsin A, TTF1, TP63, and MUC1 protein in said sample; wherein the Napsin A fragment peptide is the peptide of SEQ ID NO:5 and MUC1 peptide is the peptide of SEQ ID NO:6, wherein said amount is a relative amount or an absolute amount, and whereby when one, two, three or four of said KRT7, MUC1, TTF1, and Napsin A fragment peptides are detected and one or both of said KRT5 and TP63 fragment peptides are not detected is indicative of adenocarcinoma and whereby when one or both of KRT5 and TP63 proteins are detected and KRT7, MUC1, TTF1, and Napsin A are not detected is indicative of squamous cell carcinoma.