Patent ID: 9494519
Filing Date: 2016-11-15
CPC Classification: B01L,C12Q,G01N,Y10T

Claim Text:
1. A method for simultaneously amplifying of a plurality of loci in a nucleic acid solution comprising: providing in single solutions contained in a plurality of reaction chambers located in a biochip, samples having at least ten and up to 250 target nucleic acid loci to be amplified, with at least ten and up to 250 different primer pairs, each primer pair hybridizing to one of the at least ten and up to 250 loci to be amplified, said solution further comprising: providing a TCE, comprising a means for heating and cooling, at least one thermosensor, a controller that receives signals from said at least one thermosensor and a power supply, said at least one thermosensor positioned and configured to measure the effective temperature of each of the single solutions in the reaction chambers of the biochip and to provide feedback to the TCE to heat or cool the solution to set or maintain the solution at a desired temperature; simultaneously amplifying said plurality of loci by using the at least one thermosensor, the controller and the TCE to sequentially thermally cycle the temperature of the nucleic acid solution in each reaction chamber between a denaturing state, an annealing state, and an extension state for a predetermined number of cycles at heating and cooling rates of about 4-150° C./sec, and minimizing the transition times between the extension, denaturation, and annealing states to yield a plurality of amplified loci in each reaction chamber in 97 minutes or less.