Patent ID: 9238834
Filing Date: 2016-01-19
CPC Classification: C12Q

Claim Text:
1. A method for sequencing a target nucleic acid comprising: (a) sequencing the target nucleic acid to produce primary sequence information for the target nucleic acid; (b) identifying missing sequences and/or low confidence sequences in the target nucleic acid from the primary sequence information determined in step (a); (c) synthesizing a plurality of target-specific oligonucleotides, each corresponding to a sequence identified in step (b); (d) hybridizing said plurality of target-specific oligonucleotides to a library of fragments of the target nucleic acid; (e) hybridizing universal oligonucleotides to the library of fragments; (f) ligating the universal oligonucleotides that have hybridized to the target nucleic acids to adjacently hybridized target-specific oligonucleotides, thereby producing universal oligonucleotide:target-specific oligonucleotide ligation products; and (g) preparing an enriched library of fragments of the target nucleic acid by capturing fragments of the target nucleic acid from the library that are hybridized to the universal oligonucleotide:target-specific oligonucleotide ligation products.