Patent ID: 9410127
Filing Date: 2016-08-09
CPC Classification: B01L,C12N

Claim Text:
1. A microfluidic apparatus mounted on a rotation driving unit, the microfluidic apparatus comprising: a sample chamber that receives a biological sample and separates the biological sample into a first fluid layer comprising a first target material and a second fluid layer comprising a second target material using centrifugal force; a first sample channel connected to the sample chamber at a first location on the sample chamber; a second sample channel connected to the sample chamber at a second location on the sample chamber, wherein the first location is located further away from a rotation center of the apparatus than the second location; a first enriching unit connected to the sample chamber by the first sample channel and comprising a first fine particle that specifically binds to the first target material, wherein the first enriching unit receives the first fluid layer from the sample chamber via the first sample channel, wherein the first fine particle and the first target material form a first complex comprising the first fine particle and the first target material, and wherein the first enriching unit separates the first complex from the first fluid layer using a density difference with a density-gradient material (DGM) having a lower density than the first complex; and a second enriching unit connected to the sample chamber by the second sample channel, wherein the second enriching unit receives the second fluid layer from the sample chamber via the second sample channel, forms a second complex of a second fine particle and the second target material, and separates the second complex from the second fluid layer using a density difference between the second fluid layer and the second complex.