Patent ID: 9465036
Filing Date: 2016-10-11
CPC Classification: G01N,H01J,Y10T

Claim Text:
1. A method of producing polymeric particles incorporating in the particles two or more staining elements to facilitate determination of said particles by FC-MS, said method comprising: (a) combining two or more staining element-containing complexes with a solvent mixture comprising at least one organic monomer in which the two or more staining element-containing complexes are soluble and at least one different solvent in which said at least one organic monomer and said two or more staining element-containing complexes are less soluble, wherein at least one of said two or more staining element-containing complexes comprises a tris-4,4,4-trifluoro-1-(2-naphthyl-1,3-butanedione) ligand, (b) emulsifying the mixture of step (a) for a period of time sufficient to provide a uniform emulsion; (c) initiating polymerization and continuing reaction until a substantial portion of monomer is converted to polymer; and (d) incubating the product of step (c) for a period of time sufficient to obtain a latex suspension of stained polymeric particles with the two or more staining element-containing complexes incorporated in the particles therein, wherein said two or more staining element-containing complexes are selected such that upon FC-MS interrogation of each individual particle of said stained polymeric particles, a distinct MS signal is obtained from said two or more staining element-containing complexes, wherein the intensity of said distinct MS signal is proportional to the amount of said two or more staining element-containing complexes in said individual particle of said stained polymeric particles.