Patent ID: 9399664
Filing Date: 2016-07-26
CPC Classification: C07K

Claim Text:
1. A process for purification of pneumocandin B 0 with at least 90% purity, the process consisting of: a) extracting pneumocandin B b) washing the concentrated extract with a second solvent selected from the group consisting of water, petroleum ether and cyclohexane to obtain an organic layer; c) charcoalizing and filtering the organic layer through a celite bed to obtain a filtrate; d) crystallizing the filtrate to obtain a solid, wherein the crystallizing is carried out by solvent-antisolvent method, wherein the solvent is selected from group consisting of methanol, n-propanol, iso-propanol, n-butanol, sec-butanol, tertiary-butanol and any combinations thereof and the antisolvent is selected from group consisting of acetone, acetonitrile, pet-ether, cyclohexane, ethyl acetate, water and heptane or any combinations thereof; e) loading the solid in a column with an adsorbent; f) eluting with 100% water first, followed by alkanol/water, followed by 100% alkanol to obtain eluted pneumocandin B g) concentrating and crystalizing the eluted pneumocandin B