Patent ID: 9494606
Filing Date: 2016-11-15
CPC Classification: G01N

Claim Text:
1. A method of detecting an apolipoprotein in a sample, the method comprising (a) contacting a sample comprising a lipoprotein particle with a first binding agent, wherein the lipoprotein particle comprises an integral apolipoprotein and may or may not also comprise a non-integral apolipoprotein, wherein the first binding agent specifically binds a non-integral apolipoprotein on the lipoprotein particle, and wherein the first binding agent is selected from the group consisting of antibody, antibody fragment, aptamer and adnectin; (b) removing the sample of (a) from contact with the first binding agent bound to the non-integral apolipoprotein-containing lipoprotein particle; (c) contacting the sample of (b) with a second binding agent that specifically binds an integral apolipoprotein, wherein the second binding agent is selected from the group consisting of antibody, antibody fragment, aptamer and adnectin; (d) dissociating the non-integral apolipoprotein-containing lipoprotein particle of (a) into two or more dissociated components, wherein the dissociated components comprise i) the integral apolipoprotein and ii) the first binding agent of (a) bound to the non-integral apolipoprotein; (e) contacting the dissociated components of (d) with a third binding agent that specifically binds an integral apolipoprotein, wherein the third binding agent is selected from the group consisting of antibody, antibody fragment, aptamer and adnectin; and (f) detecting an apolipoprotein in or on a lipoprotein particle bound to the second binding agent of (c) and/or an apolipoprotein bound to the third binding agent of (e).