Patent ID: 9428548
Filing Date: 2016-08-30
CPC Classification: C07K

Claim Text:
1. A method for purifying a polypeptide comprising a C H 2/C H 3 region, comprising the steps of: (a) binding the polypeptide to Protein A; and (b) eluting the polypeptide with a pH gradient starting at or below 5.0 using an elution buffer, wherein the elution buffer contains a high pH buffer and a low pH buffer and wherein the pH gradient is formed by adjusting a percentage of each pH buffer in the elution buffer, wherein the high pH buffer is at about 5.0 and wherein the low pH buffer is at about 2.7, wherein the pH gradient ends at about 3.7, and wherein an aggregate, a host cell impurity, a virus filter foulant, a virus particle and a virus-like particle are removed from the desired polypeptide.