Patent ID: 9481718
Filing Date: 2016-11-01
CPC Classification: A61K,C07K

Claim Text:
1. A method for extracting hirudin from leeches in vivo, the method comprising following steps in a chronological order: (1) contacting the leeches with inducing membranes containing an inducing agent for from about 10 minutes to 60 minutes, the inducing agent comprising an aqueous solution containing about 3% w/v glycine, about 3% alanine, about 3% w/v uridine monophosphosphate, about 5% w/v trehalose, and about 8% w/v glucose, the inducing membranes made from at least one of large intestine, small intestine, or casings, the leeches comprising a (2) removing the inducing membranes from the leeches; (3) contacting the leeches with a regurgitation agent for from about 10 minutes to 30 minutes in a container such that the leeches are induced to regurgitate regurgitation contents at a room temperature, the regurgitation agent containing 50% ethanol, the regurgitation contents containing unrefined hirudin; (4) collecting the regurgitation contents containing unrefined hirudin; (5) purifying the regurgitation contents to obtain refined hirudin; and (6) culturing the leeches without feeding food to the leeches for a predetermined time period after the collecting the regurgitation contents.