Patent ID: 9458490
Filing Date: 2016-10-04
CPC Classification: C12Q

Claim Text:
1. A method to detect microbes from water samples using cultivation, said method comprising the steps of: a) collecting at least one microbiological sample in at least one container; b) introducing a gas into said container to promote microbe growth in said sample collected in said container; c) performing step b) at a time selected from the group consisting of prior to said collecting of said sample, and during said collection of said sample; d) starting said microbe growth in said sample in a static cultivation to maximize a division of microbe cells in said sample during a time period to reduce environmental stress on said sample when said sample interacts with said gas; e) continuing said static cultivation in a Portable Microbe Enrichment Unit (PMEU) device with an application of a cultivation gas flow so that a transfer from said gas used in said step (b) to said cultivation gas flow is at a condition determined to said microbe cells in a static state; f) monitoring at least one hygiene indicator; and g) detecting at least one microbe from said sample; wherein said gas is selected from the group consisting of carbon dioxide and hydrogen.