Patent ID: 9474254
Filing Date: 2016-10-25
CPC Classification: A01K,C12N

Claim Text:
1. A method of introducing a transgene or a mutation into the genome of a male rat wherein said male rat is DAZL-deficient, comprising: a) providing a cultured rat spermatogonial stem cell line of a predetermined genetic background; (b) genetically modifying the rat spermatogonial stem cell line by gene-trap insertion mutagenesis with a transposon plasmid containing a gene-trap selection cassette comprising transposase binding sites necessary for transposition to generate a rat donor haplotype, wherein a transposon insertion site can be remobilized by the expression of a transposase recognizing the transposase binding sites in the stem cell line in culture or at a later stage in vivo; (c) transplanting the genetically modified rat spermatogonial stem cell line of a predetermined genetic background into a testicle of the male rat; and (d) allowing a time sufficient for the genetically modified rat spermatogonial stem cell line to develop into fertilization-competent, haploid male gametes, wherein DAZL-deficient rats transmitted the rat donor haplotype more efficiently than from wildtype rat recipient testes.