Patent ID: 9476084
Filing Date: 2016-10-25
CPC Classification: C12Q

Claim Text:
1. A method for enumeration of microorganisms within a freeze-dried powder suspected of containing Lactobacilli or Bifidobacteria, the method comprising: rehydrating the powder with an MRS culture broth having a pH between about 7.2 and about 8.5 to form a rehydrated sample, wherein the powder includes alginate; holding the sample at a temperature between about 10° C. and about 35° C. for a length of time of about 20 minutes to about 60 minutes; serially diluting the sample to form a plurality of serial dilutions, wherein the serial dilutions each have a pH between about 7.2 and about 8.5; plating each serial dilution with an agar composition comprising cysteine hydrochloride in an amount of about 0.005% to about 0.5% by weight/volume percent of the agar composition to form a plurality of plates; incubating the plates; counting the colonies in each plate; and quantifying the microorganisms in the powder based on a correlation between the number of colonies and the dilution for each plate.