Patent ID: 9388463
Filing Date: 2016-07-12
CPC Classification: C07H,C12P,C12Q

Claim Text:
1. A method for controlling the incorporation of nucleotides into a polynucleotide strand, comprising the steps of: (a) providing a nucleotide having a ribose or deoxyribose sugar moiety, wherein an enzymatically removable protecting group is attached to the 3′ or the 2′ oxygen atom of the ribose or deoxyribose sugar moiety of the nucleotide; (b) providing a first enzyme to incorporate the nucleotide into the strand, whereby the protecting group prevents incorporation of another nucleotide into the strand, and detecting the incorporated nucleotide; and (c) providing a second enzyme to remove the protecting group to expose a 3′-OH group on the first nucleotide, thereby allowing incorporation of another nucleotide into the strand; (d) repeating steps (a) to (c) at least once; thereby controlling the incorporation of nucleotides into the strand; wherein the protecting group comprises a disulfide.