Patent ID: 9513293
Filing Date: 2016-12-06
CPC Classification: A61K,B82Y,C07K,G01N

Claim Text:
1. A method of detecting or diagnosing pancreatic cancer comprising: a) obtaining a sample from an individual; b) extracting the sample with an organic phase to remove an inhibitor of antibody binding to MUC-5ac; and c) performing an immunoassay with an anti-MUC-5ac antibody or antigen-binding fragment thereof that binds to the same epitope as or competes for binding to MUC-5ac with a chimeric PAM4 (cPAM4) antibody that comprises the light chain variable region CDR sequences CDR1 (SASSSVSSSYLY, (SEQ ID NO:1); CDR2 (STSNLAS, SEQ ID NO:2); and CDR3 (HQWNRYPYT, SEQ ID NO:3); and the heavy chain variable region CDR sequences CDR1 (SYVLH, SEQ ID NO:4); CDR2 (YINPYNDGTQYNEKFKG, SEQ ID NO:5) and CDR3 (GFGGSYGFAY, SEQ ID NO:6); to detect the presence of MUC-5ac in the sample; wherein the presence of MUC-5ac in the sample is indicative of pancreatic cancer in the individual.