Patent ID: 9447163
Filing Date: 2016-09-20
CPC Classification: C07K

Claim Text:
1. A chromatographic process for separating protein components said process comprising the steps of: a. applying a protein-containing solution to a column of a chromatographic solid phase material, wherein the protein-containing solution comprises an insulin peptide and one or more di- or polyvalent metal ions, wherein the loading of the insulin peptide is at least 6.0 g per liter of column volume (g/L b. eluting the insulin peptide from said solid phase material by means of an eluent having a pH of at the most 8.5; and collecting a pool of the insulin peptide corresponding to at least 75% by weight of the insulin peptide applied to the column in step (a); and wherein the insulin peptide is capable of self-association and/or structural change in the presence of the di- or polyvalent metal ions.