Patent ID: 9249465
Filing Date: 2016-02-02
CPC Classification: C12Q

Claim Text:
1. A method for detecting the presence and/or severity of lung and/or colorectal cancer, comprising the steps of: (a) obtaining a test sample of bodily fluid comprising a nucleic acid from a subject; (b) isolating RNA molecules from the sample; (c) reverse-transcribing the RNA molecules to synthesize cDNA fragments; (d) amplifying the cDNA fragments of at least six cancer gene markers with primers that comprise a fluorescent label, wherein the at least six cancer gene makers are selected from: (e) measuring the quantity of the amplified cDNA fragments of the at least six cancer gene markers; (f) normalizing the measured quantity of the amplified cDNA fragments of each of the at least six cancer gene markers to a housekeeping gene to obtain a normalized quantity of the amplified cDNA fragments of each of the at least six cancer gene markers; (g) providing a logistic regression prediction model containing a positive coefficient for MDM2, GRB2, MCM4, NF1and DUSP6 each and a negative coefficient for EIF2S3, RNF4, and MMD each; (h) applying the normalized quantity of the amplified cDNA fragments of each of the at least six cancer gene markers to the logistic regression prediction model to calculate the probability of cancer and/or cancer recurrence risk; and