Patent ID: 9499797
Filing Date: 2016-11-22
CPC Classification: C12N

Claim Text:
1. A method of producing an induced pluripotent stem cell, comprising the steps of: concurrently introducing two or more plasmids into a mammalian somatic cell in vitro, wherein the two or more plasmids encode the following reprogramming factors: Oct 3/4, Sox 2, Klf4, and a Myc family member selected from the group consisting of c-Myc, N-Myc, L-Myc and T58A mutant of c-Myc, wherein at least one plasmid vector encodes two or more of the reprogramming factors via an intervening sequence enabling polycistronic expression, and culturing the mammalian somatic cell in a medium that supports pluripotent stem cells such that iPS cells are obtained, wherein at least a portion of the one or more plasmids are not integrated into a chromosome of the somatic cell.