Patent ID: 9493814
Filing Date: 2016-11-15
CPC Classification: C12N,C12Q

Claim Text:
1. A flavin-binding glucose dehydrogenase having the following amino acid substitutions in SEQ ID NO: 1 or amino acid sequence having a 90% or more sequence identity with the amino acid sequence of SEQ ID NO: 1: the amino acid at the position corresponding to methionine at position 78 is selected from the group consisting of glutamic acid, glutamin, cysteine and asparagine, the amino acid at the position corresponding to tyrosine at position 79 is selected from the group consisting of phenylalanine and asparagine, the amino acid at the position corresponding to glutamine at position 81 is selected from the group consisting of leucine, phenylalanine and asparagine, the amino acid at the position corresponding to leucine at position 121 is selected from the group from the group consisting of cysteine and methionine, the amino acid at the position corresponding to valine at position 122 is selected from the group consisting of threonine, alanine and cysteine, the amino acid at the position corresponding to tryptophan at position 123 is selected from the group consisting of cysteine, phenylalanine, histidine, valine and serine, the amino acid at the position corresponding to glutamic acid at position 465 is selected from the group consisting of arginine and aspartic and, the amino acid at the position corresponding to tryptophan at position 569 is tyrosine, the amino acid at the position corresponding to serine at position 612 is selected from the group consisting of cysteine and threonine, the amino acid at the position corresponding to valine at position 232 in the amino acid sequence of SEQ ID NO: 1 is glutamic acid, the amino acid at the position corresponding to threonine at position 387 is alanine, the amino acid at the position corresponding to isoleucine at position 545 is threonine, and the amino acid at the position corresponding to tryptophan at position 569 is tyrosine, the amino acid at the position corresponding to valine at position 232 in the amino acid sequence of SEQ ID NO: 1 is glutamic acid, the amino acid at the position corresponding to threonine at position 387 is alanine, the amino acid at the position corresponding to isoleucine at position 545 is threonine, and the amino acid at the position corresponding to serine at position 612 is cysteine, the amino acid at the position corresponding to valine at position 232 in the amino acid sequence of SEQ ID NO: 1 is glutamic acid, the amino acid at the position corresponding to threonine at position 387 is alanine, the amino acid at the position corresponding to isoleucine at position 545 is threonine, and the amino acid at the position corresponding to serine at position 612 is threonine, the amino acid at the position corresponding to valine at position 232 in the amino acid sequence of SEQ ID NO: 1 is glutamic acid, the amino acid at the position corresponding to threonine at position 387 is alanine, the amino acid at the position corresponding to glutamic acid at position 465 is aspartic acid, the amino acid at the position corresponding to isoleucine at position 545 is threonine, and the amino acid at the position corresponding to tryptophan at position 569 is tyrosine, the amino acid at the position corresponding to tryptophan at position 569 is tyrosine and the amino acid at the position corresponding to methionine at position 78 is glutamic acid, the amino acid at the position corresponding to trytophan at position 569 is tyrosine and the amino acid at the position corresponding to methionine at position 78 is asparagine, the amino acid at the position corresponding to tryptophan at position 569 is tyrosine and the amino acid at the position corresponding to leucine at position 121 is methionine, the amino acid at the position corresponding to tryptophan at position 569 is tyrosine and the amino acid at the position corresponding to tryptophan at position 123 is phenylalanine, the amino acid at the position corresponding to tryptophan at position 569 is tyrosine and the amino acid at the position corresponding to tryptophan at position 123 is valine, the amino acid at the position corresponding to tryptophan at position 569 is tyrosine and the amino acid at the position corresponding to serine at position 612 is cysteine, or the amino acid at the position corresponding to tryptophan at position 569 is tyrosine and the amino acid at the position corresponding to serine at position 612 is threonine.