Patent ID: 9283264
Filing Date: 2016-03-15
CPC Classification: A61K,A61P

Claim Text:
1. A method, comprising providing a composition comprising recombinant paramyosin, contacting said composition with a strand separation agent to yield a solution of paramyosin monomers, removing low molecular weight moieties from said solution of monomers, and removing said strand separation agent to yield a product comprising purified full length Schistosomal paramyosin coiled coil dimer, wherein said paramyosin is selected from the group consisting of Schistosoma japonicum 97 (Sj97) and Schistosoma mansoni (Sm97), and wherein said strand separation agent comprises Guanidium Hydrochloride (Gu-HCl) with a concentration of about 7M, urea with a concentration of at least 9M, or an ionic detergent, wherein said ionic detergent comprises at least 0.02% (w/w) sodium dodecyl sulfate (SDS), at least 0.2% (w/w) N-lauryl sarcosine, or deoxycholic acid.