Patent ID: 9329130
Filing Date: 2016-05-03
CPC Classification: G01J,G01N

Claim Text:
1. A system for imaging microscopic objects, comprising: a covered sample chamber having a fixed depth ranging from about 1 μm to about 200 μm, the covered sample chamber being configured to hold about 1 μL to about 1,000 μL of a liquid suspension of microscopic objects to be imaged in a liquid sample, wherein the covered sample chamber comprising an optically clear window allowing exposure of the liquid sample; at least one fluorescent light source capable of providing a fluorescent excitation light beam to the liquid sample through the window; a bright-field light source capable of providing a bright-field light beam to the liquid sample; and at least one light detection device for detecting a light signal from the liquid sample thereby forming at least one image of the microscopic objects, wherein the fluorescent excitation light beam is at an oblique angle to the bright-field light beam and is oblique to the window's plane of surface, and the fluorescent excitation light beam comprises a wavelength ranging from about 300 nm to about 10,000 nm.