Patent ID: 9458494
Filing Date: 2016-10-04
CPC Classification: C12N,C12Q

Claim Text:
1. A method for extracting nucleic acids from a wax embedded sample, comprising: (a) contacting the sample with a wax-solubilizing agent comprising at least one organic solvent immiscible with water, wherein the at least one organic solvent comprises tetradecane, pentadecane, or hexadecane to form a mixture, (b) optionally incubating the mixture obtained in step (a), (c) adding an aqueous lysis buffer to the mixture still comprising the wax-solubilizing agent, and (d) incubating the mixture obtained in step (c) to obtain a bi- or multi-phasic mixture, comprising (i) at least an organic phase, said organic phase consisting essentially of the dissolved wax and the wax-solubilizing agent, and (ii) an aqueous phase, said aqueous phase comprising the nucleic acids, thereby extracting nucleic acids from the sample, wherein steps (a) and (b) are optionally carried out as one combined step.