Patent ID: 9249388
Filing Date: 2016-02-02
CPC Classification: A61K,A61P,C12N,G01N

Claim Text:
1. A method for producing pluripotent cells that can be used to produce differentiated cells in vitro comprising: (a) obtaining an oocyte in metaphase II, which optionally may be genetically modified; (b) activating said oocyte by a method selected from the group consisting of (1) culturing the oocyte under conditions that do not result in second polar body extrusion; (2) culturing the oocyte in the presence of an agent that inhibits polar body extrusion, and (3) culturing the oocyte under conditions that prevent the initial cleavage; (c) culturing said activated oocyte to produce a gynogenetic embryo comprising a discernable trophectoderm and an inner cell mass; (d) isolating said inner cell mass or cells derived therefrom and transferring said inner cell mass or cells derived therefrom to an in vitro media that inhibits differentiation; and (e) culturing said inner cell mass or cells derived therefrom to maintain said inner cell mass or cell derived therefrom in an undifferentiated pluripotent state; wherein the activation conditions include use of a compound that inhibits microfilament or protein production.