Patent ID: 9528131
Filing Date: 2016-12-27
CPC Classification: C12N,C12P,C12Y,Y02E,Y02P

Claim Text:
1. A method for stereoselectively reducing 2,6-dichloro-3′-fluoroacetophenone substrate, optionally substituted at one or more positions selected from the group consisting of 4′ and 5′, to the corresponding substituted (S)-1-phenethanol, which comprises contacting the substrate with an engineered ketoreductase under reaction conditions suitable for stereoselectively reducing or converting the substrate to the corresponding substituted (S)-1-phenethanol product, wherein the engineered ketoreductase is derived from a wild-type Lactobacillus ketoreductase and wherein said engineered ketoreductase comprises an amino acid sequence at least 90% identical to the reference sequence of SEQ ID NO: 2, 4, or 98; and wherein said engineered ketoreductase is capable of stereoselectively reducing 2,6-dichloro-3′-fluoroacetophenone to (S)-1-(2,6-dichloro-3-fluorophenyl) ethanol.