Patent ID: 9518993
Filing Date: 2016-12-13
CPC Classification: C07K,G01N

Claim Text:
1. A method of determining a haptoglobin (Hp) phenotype of a subject, comprising: contacting a biological sample of said subject with an isolated anti-haptoglobin monoclonal antibody, wherein the isolated anti-haptoglobin monoclonal antibody, comprises at least one light chain variable region and at least one heavy chain variable region, said light chain variable region comprising: the complementarity determining region light chain (CDRL) 1 consisting of SEQ ID NO:3; a CDRL2 consisting of SEQ ID NO:4; and a CDRL3 consisting of SEQ ID NO:5, and said heavy chain variable region comprising: a complementarity determining region heavy chain (CDRH) 1 consisting of SEQ ID NO:8; a CDRH2 consisting of SEQ ID NO:9; and a CDRH3 consisting of SEQ ID NO:10; forming a bound complex between the isolated anti-haptoglobin monoclonal antibody and haptoglobin in the biological sample; quantitatively determining a binding affinity between said haptoglobin and said isolated anti-haptoglobin monoclonal antibody; and comparing said quantitatively determined binding affinity with a value obtained from quantitatively determining the binding affinity of said isolated anti-haptoglobin monoclonal antibody to an isolated Hp 1-1, Hp 2-1, or Hp 2-2 isoform, wherein the binding affinity determined is indicative of the Hp allele type, thereby determining the phenotype of Hp in said subject.