Patent ID: 9435743
Filing Date: 2016-09-06
CPC Classification: C12N,C12Q,G01N

Claim Text:
1. A method for detecting a target molecule, comprising: a) contacting an immobilized target molecule with a modified bacteriophage comprising SEQ ID NO: 1, SEQ ID NO:2, SEQ ID NO:3, or SEQ ID NO:4, wherein the bacteriophage specifically binds the target molecule, under conditions that promote binding of the bacteriophage to the target molecule, to produce a target molecule-bacteriophage complex; b) contacting the target molecule-bacteriophage complex with a bacterial strain susceptible to infection by the bacteriophage; c) incubating the target molecule-bacteriophage complex with the bacterial strain under conditions that promote the propagation of the bacteriophage in the bacteria and expression of the luciferase, to produce a bacteriophage-infected culture; d) adding to the bacteriophage-infected culture a substrate for the luciferase; and e) measuring the presence and/or magnitude of fluorescence produced from the action of the luciferase on its substrate.