Patent ID: 9322054
Filing Date: 2016-04-26
CPC Classification: B01L,C12P,C12Q,G01N

Claim Text:
1. A microfluidic cartridge, comprising: at least one nucleic acid analysis portion, each nucleic acid analysis portion including: a fluidic network defined within the nucleic acid analysis portion, the fluidic network being configured for micro-liter volumes or less, the fluidic network including at least one frangible seal formed from a depression defined in a fluidic channel with a fluid flow resistance determined by a depth and a width of the depression; a sample input at a beginning of the fluidic network, the sample input having a fitting that is configured to be mated to a complementary fitting of a sample acceptor to form a fluid-tight seal; a plurality of vent ports and fluidic channels in the fluidic network configured to effectuate hydrodynamic movement within the fluidic network; an extraction mixture reservoir in the fluidic network, the extraction mixture reservoir being configured to hold an enzymatic mixture for performing nucleic acid extraction on a sample provided by the sample acceptor; a mixing chamber in the fluidic network, the mixing chamber being configured to mix amplification reagents and a portion of an extracted nucleic acid mixture; and an amplification chamber in the fluidic network, the amplification chamber being configured to hold an amplification mixture during nucleic acid amplification.