Patent ID: 9453845
Filing Date: 2016-09-27
CPC Classification: B01D,G01N,H01J

Claim Text:
1. A method for determining the presence of at least one distinct polypeptide in a biological sample, wherein the at least one distinct polypeptide contains a mutation or post-translational modification relative to a wild type or unmodified polypeptide, respectively, the method comprising: a. Contacting the biological sample with a hydrolyzing agent, wherein the hydrolyzing agent is capable of hydrolyzing the distinct polypeptide in a sequence-specific manner such that at least one distinct peptide, comprising said mutation or post-translational modification and having a predetermined peptide measured accurate mass, would result if the at least one distinct polypeptide were present in the biological sample, to obtain a hydrolyzed sample; b. Bringing the hydrolyzed sample in contact with a substrate comprising at least one immobilized binding partner, wherein the at least one immobilized binding partner is capable of specifically binding the distinct peptide; c. Removing the hydrolyzed sample from the substrate in a manner such that the distinct peptide would remain bound to the immobilized binding partner; d. Contacting the substrate with an elution solution, wherein the distinct peptide would dissociate from the immobilized binding partner into the elution solution, to obtain an elution solution comprising a plurality of molecules; e. Segregating the plurality of molecules by liquid chromatography to obtain sorted molecules; f. Determining the measured accurate mass of at least one sorted molecule present in the elution solution; and g. Determining the presence of the at least one distinct polypeptide in the biological sample when a measured accurate mass of at least one sorted molecule is substantially equal to the predetermined peptide measured accurate mass of the at least one distinct peptide.