Patent ID: 9512406
Filing Date: 2016-12-06
CPC Classification: A61K,C12N

Claim Text:
1. A method comprising administering to a subject one or more immature hepatocytes generated as follows: (a) incubating one or more cells with a first composition comprising an epidermal growth factor (EGF), a basic fibroblast growth factor (bFGF), an activator of WNT signaling, a nuclear receptor liver receptor homolog 1 agonist, a histone deacetylase (HDAC) inhibitor, a histone demethylase LSD1 inhibitor, and a DNA methyltransferase (DNMT) inhibitor to generate induced multipotent cells; (b) incubating the induced multipotent cells with a second composition comprising an activator of WNT signaling, a nuclear receptor liver receptor homolog 1 agonist, a histone deacetylase (HDAC) inhibitor, a histone demethylase LSD1 inhibitor, a DNA methyltransferase (DNMT) inhibitor, Activin A, or any combination thereof to generate one or more endodermal progenitor cells; (c) optionally incubating the one or more endodermal progenitor cells in a third composition comprising epidermal growth factor (EGF), basic fibroblast growth factor (bFGF), and a TGF-beta inhibitor for a time and in a sufficient amount of the third composition to generate an endodermal progenitor cell population; and (d) incubating the one or more endodermal progenitor cells or the endodermal progenitor cell population in a fourth composition comprising basic fibroblast growth factor (bFGF), a TGF-beta inhibitor, bone morphogenetic protein 4 (BMP4), and dexamethasone for a time and in a sufficient amount of the composition to generate one or more immature hepatocytes.