Patent ID: 9422603
Filing Date: 2016-08-23
CPC Classification: C12Q

Claim Text:
1. A method of forming a messenger RNA (mRNA) primer extension product and a micro RNA primer extension product in the same reaction mixture comprising; forming a reverse transcription reaction mixture comprising, a hot start mRNA primer, a micro RNA primer, a target mRNA, a target micro RNA, and a reverse transcribing enzyme, wherein the hot start mRNA primer comprises a self-complementary stem, and a loop, wherein a contiguous portion of the stem and loop forms a target specific region; hybridizing the micro RNA primer to the target micro RNA at a low temperature; extending the micro RNA primer at the low temperature to form a micro RNA extension product, wherein the self-complementary stem of the hot start mRNA primer is non-denatured; raising the temperature to a high temperature, wherein the self-complementary stem of the hot start mRNA primer is denatured; hybridizing the target specific region of the hot start mRNA primer to the target mRNA at the high temperature; extending the hot start mRNA primer to form a mRNA extension product; and, forming the mRNA primer extension product and the micro RNA primer extension product in the same reaction mixture.