Patent ID: 9234875
Filing Date: 2016-01-12
CPC Classification: C07K,G01N

Claim Text:
1. A method of purifying at least one cellular component from a biological sample comprising one or more cells or a lysate thereof, wherein the one or more cells or lysate thereof comprises a plurality of cellular components of the one or more cells, the method comprising, providing into a chamber the sample; and generating a pH gradient or pH step in the chamber with one or more proton injector(s) and/or hydroxide injector(s), such that at least two different cellular components from the plurality of cellular components are positioned in different positions in the chamber based on the isoelectric point (pI) of the at least two different components, thereby purifying at least one cellular component from a different cellular component, wherein the plurality of cellular components are electrophoretically moved through the inside of the chamber.