Patent ID: 9249455
Filing Date: 2016-02-02
CPC Classification: C12Q

Claim Text:
1. A method comprising: (a) contacting a sample to be tested for the presence or absence of a target small RNA with: wherein the tail regions of the first primer, the second primer, or both the first primer and the second primer comprise a first label and a first non-natural base; (b) extending the first primer under conditions suitable to produce a first reaction product of the small RNA, if present in the sample; (c) amplifying the first reaction product with the first primer and the second primer under conditions suitable to produce an amplification product, wherein a second non-natural base conjugated to a second label is incorporated into the amplification product opposite the first non-natural base, and wherein the first non-natural base is iso-C or iso-G and the second non-natural base is the other of iso-C or iso-G; and (d) detecting the amplification products produced in step (c) by observing a signal from the first label, the second label, or both the first label and the second label to determine the presence or absence of the target small RNA in the sample.