Patent ID: 9453844
Filing Date: 2016-09-27
CPC Classification: G01N,Y10T

Claim Text:
1. A method of selectively detecting S-sulfhydration of cysteine residues in a protein, polypeptide or peptide, comprising i) exposing said protein, polypeptide or peptide to a first reagent that forms a covalent bond with —SH and —SSH groups, thereby forming disulfide adducts; then ii) exposing said protein, polypeptide or peptide to a second reagent comprising a nucleophile that selectively reacts with disulfide adducts formed from persulfide groups, wherein said second reagent further comprises a reporting molecule linked to said nucleophile via a linking molecule; and then iii) detecting said reporting molecule, wherein positive detection of said reporting molecule indicates that said cysteine residues of said protein, polypeptide or peptide were S-sulfhydrated.