Patent ID: 9259442
Filing Date: 2016-02-16
CPC Classification: A61K,A61L,A61P,C12N

Claim Text:
1. A method for obtaining non-embryonic mammalian stem cells comprising the steps of: a) collecting a first blood sample from a mammalian subject; b) preparing an autologous platelet-rich plasma (PRP) from the first blood sample; c) activating said autologous PRP by addition of an effective amount of soluble human thromboplastin to obtain a first fluid containing concentrated activated autologous PRP; d) selecting in the mammalian subject a target injection area comprising non-embryonic stem cells, wherein the target injection area is selected from bone marrow or fat tissue; e) injecting the first fluid into the target injection area of the mammalian subject to locally induce in said mammalian subject in vivo proliferation of the non-embryonic stem cells; and f) after an incubation time, collecting from the mammalian subject injected with the first fluid a second blood sample and/or a bone marrow sample comprising stem cells and recovering the stem cells from the second blood sample and/or the bone marrow sample, wherein the non-embryonic stem cells include mesenchymal stem cells and endothelial progenitor cells.