Patent ID: 9422580
Filing Date: 2016-08-23
CPC Classification: C12N,C12P,C12Y,Y02E,Y02P

Claim Text:
1. A method for the biosynthesis of butadiene comprising converting a butanol or butene to 3-buten-2-ol using (a) a polypeptide having the activity of a desaturase or (b) using a polypeptide having the activity of a monooxygenase to form a first terminal vinyl group, wherein said desaturase or monooxygenase forms a first vinyl group, and wherein said desaturase is a cytochrome P450 or said monooxygenase is a gene product of mdpJ, and forming a second terminal vinyl group in the 3-buten-2-ol using a polypeptide having the activity of a dehydratase in enzyme class EC 4.2.1 capable of accepting a hydroxylated substrate to biosynthesize butadiene.