Patent ID: 9523677
Filing Date: 2016-12-20
CPC Classification: G01N,Y10T

Claim Text:
1. A flow cytometer system for verifying preparation of a sample fluid, prepared with a reference bead concentration, the system comprising: a sample injection probe, in communication with the sample fluid, for allowing the sample fluid with the reference bead concentration to flow through the system; an interrogation zone including a pathway fluidly coupled to the sample injection probe; a pump system fluidly coupled to the pathway and configured to draw the sample fluid with the reference bead concentration from the sample injection probe and through the interrogation zone, wherein the pump system includes a sheath pump, a waste pump, and at least one sensor, in communication with fluid flow associated with at least one of the sample injection probe, the sheath pump, and the waste pump, that provides a flow dataset indicative of sample fluid volumetric flow through the interrogation zone; a light source that illuminates the sample fluid, with the reference bead concentration, within the interrogation zone; a photodetector that outputs signals upon detection of light from particles, including the reference bead concentration, illuminated by the light source within the interrogation zone; and a processor in communication with the pump system and the photodetector and configured to direct drawing of the sample fluid through the interrogation zone, the processor configured to: