Patent ID: 9488665
Filing Date: 2016-11-08
CPC Classification: G01N

Claim Text:
1. A method for detecting the presence or absence of an antigen on a blood cell in a liquid medium containing interfering proteins which comprises: 1. providing in a vessel, a mixture of: 2. allowing a complex of b) and d) to form in a negative test and a complex of b), d) and c) to form in a positive test, all in said first liquid zone, 3. applying a magnetic field to the complexes formed in Step 2 to move them into the second, denser, liquid zone and thereafter moving the complexes into contact with said immobilized ASG therein, wherein said ASG attaches to the antibody portion c) of said complex b) d) and c) if said antibody portion is present, thereby immobilizing said complex of b), d) and c) at said ASG, 4. removing the magnetic field from the immobilized complex, whereby any b) d) complex that may have formed, comprising any unreacted magnetic particles not bound at the ASG site, are dislodged from said ASG site due to the repugnant nature and buoyant effect of said second zone liquid on said unreacted unbound magnetic particles, 5. observing the immobilized ASG surface for the presence or absence of the fixation of cells, a positive test for said antigen sought to be determined being indicated by the fixation of cells.