Patent ID: 9353343
Filing Date: 2016-05-31
CPC Classification: B01L,C12M,C12Q

Claim Text:
1. A microfluidic device comprising: a first flow channel containing a first substance flowing through; a first perfusion barrier separating the first flow channel from a cell chamber, the first substance perfusing through the first perfusion barrier into the cell chamber; a second perfusion barrier opposite from said first perfusion barrier and separating the cell chamber from a second flow channel, the second flow channel containing a second substance flowing through, the second substance perfusing through the second perfusion barrier into the cell chamber; a gradient formed within the cell chamber comprising the first substance perfusing through the first perfusion barrier and the second substance perfusing through the second perfusion barrier; such that a plurality of cells within the cell chamber are exposed to the gradient of the first substance and the second substance.