Patent ID: 9399773
Filing Date: 2016-07-26
CPC Classification: C12N

Claim Text:
1. A method of producing microRNA precursor (pre-miRNA) containing a shared sequence SEQ ID NO: 3 capable of reprogramming the malignant properties of human cancer cells into a low-grade benign or normal-like state, comprising: (a) providing at least a chemical inducer agent, containing a molecular structure similar to of 3-morpholinopropane-1-sulfonic acid (MOPS), ethanol, glycerin, or a mixture thereof; (b) providing at least a line of transformed prokaryotic cells that carry at least a plasmid vector containing at least a eukaryotic pol-2 or pol-2-like RNA promoter and are capable of expressing said pre-miRNA containing the sequence SEQ ID NO: 3 through the eukaryotic promoter, wherein the expression of said pre-miRNA from said plasmid vector is inducible by the at least a chemical agent provided in (a), and wherein said transformed prokaryotic cells are (c) mixing the at least a chemical agent from (a) and the at least a line of transformed prokaryotic cells from (b) together under a condition to induce the eukaryotic promoter-driven transcription of said pre-miRNA.