Patent ID: 9321997
Filing Date: 2016-04-26
CPC Classification: A61K,A61P,C12N,C12Q,G01N

Claim Text:
1. A method of modeling or producing a cardiac tissue comprising: (a) obtaining cardiomyocytes, wherein a first population of pluripotent cells is differentiated into said cardiomyocytes, wherein said differentiation includes forming embryoid bodies and isolating cardiomyocytes from said embryoid bodies; (b) obtaining fibroblasts, wherein a second population of pluripotent cells is differentiated into said fibroblasts; (c) co-culturing the cardiomyocytes obtained in step (a) with the fibroblasts obtained in step (b); (d) allowing integration and alignment of the cardiomyocytes and fibroblasts into a viable cardiac tissue; wherein the cardiomyocytes acquire longitudinal morphology upon integration and alignment with the fibroblasts; and wherein said viable cardiac tissue exhibits contractions for at least 2 weeks.