Patent ID: 9309568
Filing Date: 2016-04-12
CPC Classification: A61K,A61P,C12N,C12Q

Claim Text:
1. A method for detecting a ligand or analyte in a sample, comprising: forming a mixture comprising said sample, a target template, and a nucleic acid comprising (i) a priming sequence that hybridizes to said target template to form a primer:template pair, and (ii) an aptamer that binds to said ligand, if said ligand is present, wherein binding of said ligand to said aptamer causes a conformational change in the nucleic acid that allows said priming sequence to hybridize to said target template; incubating said mixture under conditions which allow said priming sequence to hybridize to said target template; extending said primer sequence to synthesize an extension product that is complementary to said target template; and detecting said extension products, wherein the presence of said extension products is indicative of detection of said ligand.