Patent ID: 9474795
Filing Date: 2016-10-25
CPC Classification: A61K,A61P,Y02A

Claim Text:
1. A method for preparing a conjugate vaccine, the method comprising: reacting a polysaccharide with 1-cyano-4-dimethylammoniumpyridinium tetrafluoroborate, whereby a solution of a cyanate-activated polysaccharide is obtained; reacting a protein with carbohydrazide or a dihydrazide in the presence of 1-[3-(dimethylamino)propyl]-3-ethyl carbodiimide hydrochloride at a pH of from 6 to 7, whereby a solution of a hydrazide-activated protein is obtained; raising the pH of the solution of the hydrazide-activated protein to from 7.0 to 11; buffer exchanging the solution of the hydrazide-activated protein to a pH of from 10.0 to 11.0; reacting the cyanate-activated polysaccharide with the hydrazide-activated protein at a pH of from 6 to 8 to yield a conjugate vaccine capable of stimulating an immune response.