Patent ID: 9494573
Filing Date: 2016-11-15
CPC Classification: C12N,G01N

Claim Text:
1. A method for preparing a Tumor in Dish model comprising (a) combining normal cells consisting of isolated human umbilical vein endothelial cells, isolated human lymphatic endothelial cells, isolated human fibroblast cells, and isolated human epithelial cells and with isolated cancer cells; and (b) culturing the combination of cells in culture medium for a time sufficient to form a tumor thereby preparing a Tumor in Dish model, wherein the culture medium consists of Microvascular Endothelial Cell Growth Medium; Airway Epithelial Basal Medium; Dulbecco's Modified Eagle's Medium, Mammary Epithelial Cell Basal Medium, or Roswell Park Memorial Institute 1640 Medium; Fibroblast Growth Medium; Hydrocortisone; Fibroblast growth factor 6; R3-Insulin-Like Growth Factor-I; Ascorbic Acid; Vascular Endothelial Growth Factor; Human Epidermal Growth Factor; Heparin; Fetal Bovine Serum; Antibiotics; L-Alanyl-L-Glutamine; Extract P; Plasma Protein Fraction; Epinephrine; Transferrin; Thyroxine 3; Insulin; and B27 supplement; wherein there are no other culture medium ingredients added during the time sufficient to form a tumor.