Patent ID: 9447384
Filing Date: 2016-09-20
CPC Classification: C12N,C12Q,C12Y,G01N

Claim Text:
1. A device for detecting the presence of arsenite in a sample, comprising at least one electrode, a redox mediator, and an arsenite oxidase enzyme modified to prevent translocation to the periplasm, wherein the enzyme comprises [1] the native AioA subunit from NT-26, or a variant, homologue or derivative thereof having at least 90% sequence homology to the amino acid sequence of SEQ ID NO:4, and [2] the native AioB subunit from NT-26, or a variant, homologue or derivative thereof having at least 90% sequence homology to the amino acid sequence of SEQ ID NO:3, wherein a portion of the native AioB subunit corresponding to the translocation signal sequence, or a functional fragment thereof, is modified, and wherein said enzyme has arsenite oxidase activity.