Patent ID: 9320779
Filing Date: 2016-04-26
CPC Classification: A61K,A61P,C07K,C12N

Claim Text:
1. A freeze-dried biological glue concentrate, obtained by a process comprising the steps of: i) equilibrating an anion exchanger of weak base type with a first buffer having a predetermined ionic strength of an alkaline pH, ii) loading said anion exchanger of weak base type with a Cohn fraction I, thus allowing the retention of the biological glue, iii) eluting the biological glue with a second buffer having an alkaline pH and an ionic strength greater than said first buffer, iv) concentrating the biological glue by ultrafiltration, and v) diafiltering the biological glue solution, followed by freeze-drying of said solution, wherein said concentrate contains a mixture from 10 to 12 g/l of trisodium citrate, from 1 to 5 g/l of lysine, from 1 to 5 g/l of glycine, from 2 to 5 g/l of Tris, from 25 to 50 g/l of arginine and from 5 to 15 g/l of isoleucine.