Patent ID: 9340829
Filing Date: 2016-05-17
CPC Classification: B82Y,C12Q,G01N

Claim Text:
1. A method of detecting a target material, the method comprising: a step of preparing a complex, the complex including an aptamer to which a target material in a specimen specifically binds, a first nucleic acid fragment that has a base sequence complementary to the aptamer, and a fixing member to which a part of the aptamer and a part of the first nucleic acid fragment are fixed, in which the aptamer has a double strand-forming site capable of forming a double strand with the first nucleic acid fragment; a step of separating the first nucleic acid fragment from the double strand-forming site of the aptamer by binding the target material to the aptamer; and a step of detecting cleavage of the double strand where the first nucleic acid fragment is separated from the aptamer; wherein a base sequence of the aptamer to which the target material binds comprises at least a part of a base sequence of a double strand-forming site formed between the aptamer and the first nucleic acid fragment.