Patent ID: 9243235
Filing Date: 2016-01-26
CPC Classification: C12N,C12P,C12Y

Claim Text:
1. A recombinant protein comprising mannose-terminated N-glycans expressed by a host cell line according to a method which includes steps of: (a) introducing a nucleic acid encoding a recombinant protein into a host cell comprising a mutation in the GnT I gene leading to loss of GnT I function, wherein the host cell is a Chinese Hamster Ovary (CHO) cell selected with Ricinus communis agglutinin I (RCA-I) or a descendent thereof; and (b) expressing the recombinant protein from the host cell, wherein the expressed recombinant protein comprises mannose-terminated N-glycans and the method does not include a step of introducing functional GnT I into the host cell.