Patent ID: 9493830
Filing Date: 2016-11-15
CPC Classification: B82Y,C12Q

Claim Text:
1. A method for determining a sequence of a nucleic acid template, comprising: (I) interrogating the nucleic acid template with a plurality of probes from at least two distinguishably labeled probe families, wherein each probe of the plurality of probes comprises a scissile linkage between a constrained portion of the probe and a distal portion of the probe that is not extendable by a ligase, and wherein the distal portion of the probe further comprises a label, wherein interrogating comprises: (II) recording the ordered series of signals, corresponding to the first and second region of the template, on a computer readable medium; (III) obtaining sequence information for the first region of the template from the first series of signals and sequence information for the second region of the template from the second series of signals; and (IV) determining a sequence of the nucleic acid template based in part on the sequence information from the first and second regions.