Patent ID: 9429566
Filing Date: 2016-08-30
CPC Classification: G01N

Claim Text:
1. A method for determining whether a test compound inhibits the degradation of a Cip/Kip protein by the Ubiquitin-Proteasome system and may be useful for treating cancer, said method comprising (a) contacting said test compound with a cell expressing a fusion protein in the presence of an eukaryotic protein synthesis inhibitor, said fusion protein comprising (i) a Cip/Kip polypeptide; and (ii) a reporter protein linked to the C-terminal of said Cip/Kip polypeptide, wherein said fusion protein has a half-life that is similar to that of said Cip/Kip polypeptide, and (b) measuring a readout signal from the reporter protein, wherein a higher readout signal from the reporter protein in the presence of said test compound, relative to the readout signal in the absence of said test compound, is indicative that said test compound inhibits the degradation of a Cip/Kip protein by the Ubiquitin-Proteasome system and may be useful for treating cancer.