Patent ID: 9353375
Filing Date: 2016-05-31
CPC Classification: C12N,C12P

Claim Text:
1. A composition comprising a recombinant cell of an Escherichia coli ( E. coli ) strain or progeny thereof, wherein the cell comprises (i) one or more copies of a heterologous nucleic acid(s) encoding a 6-phosphogluconolactonase (PGL) polypeptide wherein the nucleic acid is integrated in the E. coli chromosome and (ii) one or more heterologous nucleic acid(s) encoding isoprene synthase, wherein prior to the integration, the E. coli does not contain nucleic acids(s) encoding a PGL polypeptide, and wherein the resulting cell produces isoprene at a greater titer than that of the same cells that do not comprise (i).