Patent ID: 9394538
Filing Date: 2016-07-19
CPC Classification: A61P,C12N

Claim Text:
1. A method for using a recombinant nucleic acid composition to induce tumor suppression effects in at least a targeted cancer or tumor cell, comprising the steps of: (a) providing the recombinant nucleic acid composition capable of being delivered and processed to provide at least a gene silencing effector capable of concurrently suppressing the expression of CDK2, cyclin D and BMI-1 oncogene, wherein said gene silencing effector is a hairpin RNA having a sequence consisting of SEQ ID NO: 9, SEQ ID NO: 10, SEQ ID NO: 11, SEQ ID NO: 12, SEQ ID NO: 13, SEQ ID NO: 14, SEQ ID NO: 15 and SEQ ID NO: 16 and wherein said gene silencing effector can be further processed to provide mir-302a, mir-302b, mir-302c and mir-302d in the targeted cancer or tumor cell; and (b) treating a cell substrate containing the targeted cancer or tumor cell with said recombinant nucleic acid composition under a condition that the concentration of said gene silencing effector is at least 1.3 fold higher than the natural expression level of mir-302 found in human embryonic stem cell lines H1 or H9 and is sufficient to suppress said CDK2, cyclin D and BMI-1 oncogene; wherein the cancer or tumor cell is identified as a cell type derived from human neoplastic Tera-2-derived teratocarcinomas.