Patent ID: 9394332
Filing Date: 2016-07-19
CPC Classification: C07H,C12Q

Claim Text:
1. A method for the conversion of cytosine bases in a DNA molecule to uracil bases, comprising: a) binding a double stranded DNA directly to an unmodified solid phase without a compound mediating the binding to the solid phase, wherein the unmodified solid phase is selected from the group consisting of glass, silica, and a glass or silica containing material, b) denaturing the double stranded DNA, c) incubating the solid phase bound DNA in the presence of sulfite ions whereby the DNA is deaminated, yielding a solid phase bound deaminated DNA, d) optionally washing the solid phase bound deaminated DNA, e) incubating the solid phase bound deaminated DNA under alkaline conditions whereby the deaminated DNA is desulfonated, f) washing the solid phase bound deaminated and desulfonated DNA, and g) eluting the washed deaminated and desulfonated DNA from the solid phase.