Patent ID: 9399801
Filing Date: 2016-07-26
CPC Classification: C12Q

Claim Text:
1. A method for generating at least one CRISPR variant comprising a tag, comprising the steps of: (a) exposing a parent bacterium comprising a CRISPR locus and a cas gene to at least one bacteriophage to produce a culture of bacteriophage resistant variant bacteria comprising a modified CRISPR locus, under conditions such that said modified CRISPR locus comprises an additional repeat-spacer unit, naturally inserted therein, wherein the spacer of said additional repeat-spacer unit has a length of between about 20 bp and about 58 bp and has 100% identity to a nucleotide sequence in the genome of said at least one bacteriophage wherein said additional repeat-spacer unit provides a tag; (b) selecting said bacteriophage resistant variant bacteria; (c) comparing said CRISPR locus or a portion thereof of said parent bacterium and said modified CRISPR locus of said bacteriophage insensitive variant bacteria, to identify bacteriophage insensitive variant bacteria comprising in their modified CRISPR locus, an additional repeat-spacer unit, is absent from said CRISPR locus of said parent bacterium; and (d) selecting said bacteriophage insensitive variant bacteria comprising in their modified CRISPR locus an additional repeat-spacer unit, wherein said additional repeat-spacer unit is not present in the parent bacterium; and (e) isolating and/or cloning and/or sequencing the additional repeat-spacer unit.