Patent ID: 9512465
Filing Date: 2016-12-06
CPC Classification: C12Q,G01N

Claim Text:
1. A method of differentially labeling DNA, comprising steps of: contacting a first cell with an effective amount of a first nucleoside analogue that comprises a first azide group such that the nucleoside analogue is incorporated into DNA of the first cell; contacting a second cell with an effective amount of a second nucleoside analogue that comprises a second azide group such that the nucleoside analogue is incorporated into DNA of the second cell; contacting the first cell with a first reagent comprising a first label attached to a first substituted triarylphoshine, such that a Staudinger ligation occurs between the first azide and first substituted triarylphosphine; and contacting the second cell with a second reagent comprising a second label attached to a second substituted triarylphosphine, such that a Staudinger ligation occurs between the second azide and second substituted triarylphosphine.