Patent ID: 9523132
Filing Date: 2016-12-20
CPC Classification: C12Q

Claim Text:
1. A method for rapid diagnosis of infection by a RNA virus in a sample which comprises (a) adsorbing either (b) impaling cells in said sample on the filter or surface onto an array of a plurality of vertically aligned nanostructures carrying at least one DNA reporter construct thereon, wherein the at least one DNA reporter construct encodes an mRNA viral replicon, wherein said viral replicon comprises cis-acting RNA viral replication elements and any other adjacent regions necessary for viral replication of said RNA virus flanking and operably linked to a reporter gene or operon, wherein said viral replicon lacks a nucleic acid encoding at least one factor required for viral replication and provided in trans by cells infected by said RNA virus, and wherein the viral replicon is amplified in cells infected by said RNA virus but not in cells not infected by said virus; and (c) detecting expression of said reporter gene or operon, wherein the expression of said reporter gene is indicative of a RNA virus infection.