Patent ID: 9353368
Filing Date: 2016-05-31
CPC Classification: A61P,C12N,C12Q

Claim Text:
1. A non-naturally occurring miR-196a-2 miRNA comprising a nucleic acid having a stem-loop structure comprising a miR-196a-2 scaffolding and a mature strand-star strand duplex, wherein the sequence of said mature strand of said duplex is derived from a mature endogenous miRNA but is distinct from the sequence of the endogenous mature strand of miR-196a-2, and wherein position 1 of the mature strand is U and the opposite nucleotide in the star strand is G and the duplex contains a mismatch at position 12 of the mature strand and either no additional mismatches or wobble pairs at any position other than positions 1 and 12 of the mature strand, or mismatches or wobble pairs at one or more of positions 5, 18, 19, 20, 21, 22 or 23 of the mature strand of the duplex and no mismatches or wobble pairs at any of positions 2, 3, 4, 6, 7, 8, 9, 10, 11, 13, 14, 15, 16 and 17 within the duplex, wherein positions of the mature strand are numbered relative to the 5′ end of the mature strand, the non-naturally occurring miR-196a-2 is a non-naturally occurring miRNA, and the mature strand is 19-25 nucleotides in length.