Patent ID: 9422578
Filing Date: 2016-08-23
CPC Classification: C07C,C08F,C08J,C12N,C12P,C12Y,Y02E,Y02P

Claim Text:
1. A method for biosynthesizing butadiene, comprising culturing a recombinant microbial host in a culture medium in the presence of a fermentable carbon source; enzymatically forming, in the recombinant microbial host, a first terminal vinyl group in 2-butanol using a polypeptide with the activity of a desaturase or a polypeptide with the activity of a monooxygenase to produce 3-buten-2-ol, wherein said desaturase is a cytochrome P450 or said monooxygenase is a gene product of mdpJ; and enzymatically forming, in the recombinant microbial host, a second terminal vinyl group in 3-buten-2-ol using a polypeptide with the activity of a dehydratase classified under EC 4.2.1- which is capable of accepting 3-buten-2-ol to biosynthesize butadiene by dehydration.