Patent ID: 9322829
Filing Date: 2016-04-26
CPC Classification: C07K,C12N,C40B,G01N

Claim Text:
1. A method for selecting bioactive peptides, said method comprising: a) expressing a plurality of stabilized polypeptides, wherein each said stabilized polypeptide is encoded by a different vector, and wherein each said stabilized polypeptide comprises a randomized peptide and a first stabilizing group linked to one of said randomized peptide's N- and C-termini, wherein said first stabilizing group is Xaa-Pro-, Xaa-Pro-Pro-, -Pro-Xaa, or -Pro-Pro-Xaa, wherein Xaa is alanine, wherein the first stabilizing group lacks the capacity to participate in the formation of an intramolecular disulfide bond within the polypeptide, and wherein said plurality of stabilized polypeptide comprises at least two different polypeptides; b) screening each said stabilized polypeptide of said plurality for a bioactivity; and c) selecting said bioactive peptides with said bioactivity.