Patent ID: 9493820
Filing Date: 2016-11-15
CPC Classification: C12N,C12Q,C40B,G16B

Claim Text:
1. A method for identifying one or more polymorphisms in nucleic acid samples, comprising: (a) providing a plurality of nucleic acid samples; (b) performing a reproducible complexity reduction on each of the nucleic acid samples to provide a plurality of libraries of the nucleic acid samples comprising amplified fragments, wherein said reproducible complexity reduction comprises amplifying fragments of the nucleic acid samples using at least one primer comprising at least one selective nucleotide at its 3′-end to obtain the amplified fragments, and wherein the amplified fragments in each library comprise a unique identifier sequence different from the selective nucleotide to indicate origin of each library obtained by the reproducible complexity reduction; (c) sequencing at least a portion of the libraries to obtain sequences; (d) aligning the sequences obtained in step (c) to obtain an alignment; and (e) determining one or more polymorphisms between the plurality of nucleic acid samples in the alignment of step (d).