Patent ID: 9279153
Filing Date: 2016-03-08
CPC Classification: C12Q,G01N

Claim Text:
1. A system for sequencing a polymer comprising: a nanopore substrate assembly having a donor labeled nanopore which spans a substrate, the nanopore substrate assembly having a negatively charged electrode on a cis side and a positively charged electrode on a trans side; a polymer comprising monomers each having an acceptor label, wherein the polymer is negatively charged and is loaded on the cis side of the nanopore substrate assembly, so that the labeled monomers of the polymer are translocated in single file through the nanopore from the cis side to the trans side; an energy source for exciting the nanopore donor label, wherein the nanopore donor label is configured to emit energy at a first wavelength in response to such excitation such that energy is transferred from the nanopore donor label to a monomer acceptor label whenever such monomer acceptor label exits the nanopore within an energy transfer distance of the nanopore donor label, each acceptor label being configured to emit energy at a second wavelength which is different from the first wavelength after such energy transfer; and an optical detector for detecting the energy emitted from the monomer acceptor label as a result of the transfer of energy from the nanopore donor label to the monomer acceptor label, wherein the emitted energy is associated with a single monomer and facilitates the sequencing of the polymer.