Patent ID: 9296799
Filing Date: 2016-03-29
CPC Classification: B01D,B01J,C07K,C12M

Claim Text:
1. A chromatography process for decontaminating a target protein of infectious proteins (PrP SC ) comprising the steps of contacting a potentially PrP setting buffer conditions so that the target protein binds and the PrP i) employing a pH 7.0±0.1 loading and equilibration buffer containing at least one of tri-n-butylphosphate and octoxynol (TRITON X-100) in a concentration ranging of from 0.1 to 10% (w/w), ii) employing a pH 6.5±0.1 wash buffer without tri-n-butylphosphate and/or octoxynol (TRITON X-100), and iii) employing a pH 6.5±0.1 wash buffer containing 5 to 30% (w/w) of at least one of ethylene glycol and 0.2. to 1.5 M lysine/arginine, and then to obtain the target protein in a PrP SC decontaminated fraction of HEK 293 cell-derived proteins.