Patent ID: 9404929
Filing Date: 2016-08-02
CPC Classification: C12N,G01N

Claim Text:
1. A method of identifying an antibody, or a target, comprising the steps of: a) obtaining a sample of at least 10 b) using single, massive parallel sequencing technology to directly parallel sequence within 24 hours, without any prior cloning steps, the cDNAs encoding the variable heavy (VH) and variable light (VL) chains of the immunoglobulins from the sample, thereby obtaining sequence data of the VH and VL chains of the immunoglobulins present in the sample; c) determining the frequency of the VH and VL chain sequences present in the sample by counting or statistical analysis, d) identifying the most abundant VH and VL chain sequences present in the sample according to their frequency; e) synthesizing polynucleotides encoding the most abundant VH and VL chain sequences present in the sample and producing antibodies comprising said VH and VL chains by cloning the polynucleotides into an expression vector, introducing the expression vector into a host cell and expressing the antibodies; and f) identifying the antibody(ies) specific for a target of interest or identifying the target molecule to which the antibody(ies) is specific.