Patent ID: 9416427
Filing Date: 2016-08-16
CPC Classification: A61P,C12Q

Claim Text:
1. A kit of parts for the discrimination between and quantification of a vaccine strain and a virulent strain of Marek's Disease Virus Serotype-1 (MDV-1) in a sample from a bird, the kit comprising: (i) primers that will amplify a region of the pp38 gene incorporating a consistent single nucleotide polymorphism (SNP) difference between vaccine and virulent strains of MDV-1 said SNP being a G/A polymorphism located at position 320 of the sequences of (ii) a detectable nucleic acid probe specific for the SNP of the vaccine strain of MDV-1; and (iii) at least one detectable nucleic acid probe specific for the SNP of the virulent strain of MDV-1, wherein the specific nucleic acid probes of (ii) and (iii) are each labelled with at least one signal-generating dye and wherein the probes compromise the following sequences: vaccine strain specific probe sequence: 5′ CCCACCGTGACAGCC 3′ (SEQ ID NO: 1); and at least one of the following virulent strain-specific probe sequences: 5′ CCCACTGTGACAGCC 3′ (SEQ ID NO: 2) and 5′ CTCCCACTGTGACAGCC 3′ (SEQ ID NO: 3).