Patent ID: 9404082
Filing Date: 2016-08-02
CPC Classification: C12N,G01N

Claim Text:
1. A method for producing human eosinophils from human induced pluripotent stem cells, said method comprising the steps of: (1) co-culturing, in the presence of vascular endothelial growth factor (VEGF), human induced pluripotent stem cells with stromal cells separated from the aorta gonad and mesonephros (AGM) region of a mammalian fetus; (2) performing suspension culture of the cells obtained in Step (1) using a medium comprising IL-3, IL-6, Fms-related tyrosine kinase 3 (Flt3) ligand, stem cell factor (SCF), thrombopoietin (TPO) and serum; (3) performing suspension culture of the cells obtained in Step (2) using a medium comprising IL-3, SCF, Granulocyte Macrophage colony-stimulating factor (GM-CSF) and serum; and, optionally, (4) performing suspension culture of the cells obtained in Step (3) using a medium comprising IL-3, IL-5 and serum.