Patent ID: 9410951
Filing Date: 2016-08-09
CPC Classification: B01J,C40B,G01N

Claim Text:
1. A method for producing a substrate for making a microarray, the method comprising: a step of forming a monomolecular film having orientated oxysilanyl groups toward an outmost surface on a substrate; and a step of forming a monomolecular film having orientated amino groups toward an outmost surface on the substrate by applying to the oxysilanyl groups a solution containing a diamine compound represented by formula (1): where: Z represents a cyclic divalent arylene group-containing group having 6 to 20 carbon atoms with a hydrogen atom in the group being optionally substituted by a halogen atom, a hydroxyl group, or a cyano group, and a methylene group in the group being optionally substituted by an oxygen atom (—O—) or a carbonyloxy group (—O—CO—); wherein: the step of forming the monomolecular film having orientated oxysilanyl groups toward an outmost surface on the substrate is performed by soaking the substrate in a solution containing a silane compound having an oxysilanyl group, the silane compound being represented by formula (2): wherein: m represents an integer of 3 to 16; X represents an oxysilanyl group; and Y independently represents a halogen atom or an alkoxy group having 1 to 4 carbon atoms; and a catalyst that is a nitrogen-containing organic base is mixed into the solution containing the silane compound, the catalyst being selected from the group consisting of: a. a nitrogen-containing organic base represented by the following general formula (a)-1: wherein: n′ represents 1, 2, or 3; a side chain X may be the same or different, represented by the following general formulae (X1) to (X3); a side chain Y may be the same or different, representing a hydrogen atom, or a linear, a branched, or a cyclic alkyl group having 1 to 20 carbon atoms optionally containing an ether group or a hydroxyl group; and X may form a ring by bonding with each other; wherein: R R R R b. a nitrogen-containing organic base having a cyano group represented by the following general formulae (a)−3 to (a)−6: wherein: X and n′ are as previously defined; R R c. a nitrogen-containing organic base having an imidazole skeleton and a polar functional group, as represented by the following general formula (a)-7: wherein: R R d. a nitrogen-containing organic base having a benzimidazole skeleton and a polar functional group, as represented by the following general formula (a)-8: wherein: R R e. a nitrogen-containing organic base having a polar functional group, as represented by the following general formulae (a)-9 and (a)-10: wherein: A represents a nitrogen atom or≡C—R B represents a nitrogen atom or≡C—R R R R R R f. a nitrogen-containing organic base having an aromatic carboxylate ester structure, as represented by the following general formulae (a)-11 to (a)-14: wherein: R R R R R n represents 0, 1, 2, 3,or 4; R X represents a nitrogen atom or a CR Y represents a nitrogen atom or a CR Z represents a nitrogen atom or a CR each of R g. a nitrogen-containing organic base having a 7-oxanorbornane-2-carboxylate ester structure, as represented by the following general formula (a)-15: wherein: R R R