Patent ID: 9228012
Filing Date: 2016-01-05
CPC Classification: C07K,C12N,C12Y

Claim Text:
1. A method of making a multi-subunit protein comprising the step of culturing a recombinant host cell in medium, wherein the host cell comprises a recombinant polynucleotide comprising nucleotides 1881-3656 of SEQ ID NO: 16 which encodes a mouse endoplasmic reticulum degradation-enhancing alpha-mannosidase-like protein 2 (mEDEM2) operably linked to [i] a human ubiquitin C promoter, and [ii] a polynucleotide which encodes a multi-subunit protein, wherein the multi-subunit protein is secreted by the cell into the medium, and wherein the host cell comprising said recombinant polynucleotide increases the production of multi-subunit proteins compared to a host cell lacking the overexpression of mEDEM2 under otherwise identical conditions.