Patent ID: 9493753
Filing Date: 2016-11-15
CPC Classification: C12N,C12P,C12Y,Y02P

Claim Text:
1. A modified α-glucosidase which (a) consists of an amino acid sequence which is identical to SEQ ID NO: 2 except for a substitution at a position corresponding to position 343 of SEQ ID NO: 2, or (b) comprises an amino acid sequence which has 95% or more identity with SEQ ID NO: 2 and comprises a substitution corresponding to position 343 of SEQ ID NO: 2, wherein the amino acid at position 343 is substituted by a cysteine, aspartic acid, methionine, histidine, alanine, phenylalanine, glycine, threonine, glutamic acid, valine, glutamine, asparagine or isoleucine, and wherein the modified α-glucosidase has at least one of: improved transglycosylation activity compared to the α-glucosidase of SEQ ID NO: 2, and decreased hydrolysis activity compared to the α-glucosidase of SEQ ID NO: 2.