Patent ID: 9512428
Filing Date: 2016-12-06
CPC Classification: A61K,C12N,C12Y,G01N

Claim Text:
1. A culture comprising one or more isolated mature hepatocytes, wherein after nine days of culture, at least one of the hepatocytes maintains expression levels of tyrosine aminotransferase (TAT) that is at least 80% of the level that was exhibited when the culture was established, wherein the cells are expressing an exogenous sequence comprising (a) a first microRNA comprising a core sequence that is 18 to 24 nucleotides long, wherein the 10 nucleotide sequence on the 5′ end of the core sequence comprises a seed sequence consisting of any 6, 7, 8, 9 or 10 consecutive nucleotides of SEQ ID NO:5; or (b) an external vector comprising a DNA sequence coding for the first microRNA; or (c) a second microRNA comprising a core sequence that is 18 to 24 nucleotides long; wherein the 10 nucleotide sequence on the 5′ end of the core sequence comprises a seed sequence consisting of any 6, 7, 8, 9 or 10 consecutive nucleotides of SEQ ID NO:6; or (d) an external vector comprising a DNA sequence coding for the second microRNA; or (e) two or more of (a)-(d).