Patent ID: 9291614
Filing Date: 2016-03-22
CPC Classification: B01L,G01N

Claim Text:
1. A method of assaying hemostasis, the method comprising: providing a device comprising: a) central channel having an inlet and an outlet; b) one or more outer channels adjacent to the central channel, each outer channel having an inlet and an outlet; c) a wall located between the central channel and one or more outer channels; d) at least one aperture in the wall between the central channel and one or more outer channels so as to fluidly couple the central channel and one or more outer channels; and e) a degradable matrix located in the at least one aperture so as to fluidly isolate the central channel and one or more outer channels from each other; culturing endothelial cells in the central channel; culturing tissue cells in the one or more outer channels; degrading the degradable matrix in the at least one aperture so as to fluidly couple the central channel and the one or more outer channels; flowing blood, whole or diluted, through the central channel; and determining whether or not hemostasis occurs at the at least one aperture by determining whether or not the at least one aperture becomes occluded after the degradable matrix has been degraded from the at least one aperture, and, when the at least one aperture becomes occluded, hemostasis is determined to have occurred.