Patent ID: 9347098
Filing Date: 2016-05-24
CPC Classification: C12Q

Claim Text:
1. A nucleic acid amplification reaction mixture comprising at least two primers and at least two probes, wherein: (a) the at least two primers hybridize to the same strand of a target nucleic acid sequence but each primer hybridizes to a distinct nucleotide sequence on the strand, (b) each primer is capable of simultaneously and independently producing one of at least two amplicons from the target nucleic acid sequence under amplification conditions, (c) the at least two amplicons are detectable by hybridization with the at least two probes, wherein one of the at least two probes is specific to a nucleotide sequence common to both the first and second amplicons and another of the at least two probes is specific to a nucleotide sequence contained within the second amplicon but not within the first amplicon, and each probe is detectable within a detection range C