Patent ID: 9416382
Filing Date: 2016-08-16
CPC Classification: C12N,C12P,Y02P

Claim Text:
1. A recombinant microbial cell for the production of at least one long-chain polyunsaturated fatty acid, said recombinant microbial cell comprising: (a) at least one polypeptide having acyl CoA:lysophosphatidylcholine acyltransferase (LPCAT) activity, wherein the polypeptide having LPCAT activity has at least 95% amino acid sequence identity with the polypeptide of SEQ ID NO: 4 (YILPCAT), based on the Clustal W method of alignment, and wherein the polypeptide having LPCAT activity is a polypeptide comprising at least one mutant membrane-bound O-acyltransferase protein family motif comprising the amino acid sequence set forth in SEQ ID NO: 39, wherein the amino acid residue at position 2 of SEQ ID NO: 39 is C, the amino acid residue at position 3 of SEQ ID NO: 39 is selected from the group consisting of A, C and G, the amino acid residue at position 6 of SEQ ID NO: 39 is selected from the group consisting of H, G, N, and Y, the amino acid residue at position 7 of SEQ ID NO: 39 is selected from the group consisting of A, N, G, H and M, the amino acid residue at position 16 of SEQ ID NO: 39 is selected from the group consisting of Q, N, and H, and the amino acid residue at position 17 of SEQ ID NO: 39 is selected from the group consisting of A, V and N; (b) at least one polypeptide having phospholipid:diacylglycerol acyltransferase (PDAT) activity, wherein the polypeptide having PDAT activity has at least 95% amino acid sequence identity with the polypeptide of SEQ ID NO: 32 (YIPDAT), based on the Clustal W method of alignment; and (c) a polyunsaturated fatty acid biosynthetic pathway capable of producing at least one long chain polyunsaturated fatty acid; wherein said polypeptides of (a) and (b) are expressed, and wherein the recombinant microbial cell comprises an increased amount of a polyunsaturated fatty acid measured as a weight percent of total fatty acids, when compared to a control cell, wherein the control cell is the recombinant microbial cell wherein said polypeptides of (a) and (b) are not expressed.