Patent ID: 9303285
Filing Date: 2016-04-05
CPC Classification: C12Q,G01N

Claim Text:
1. A method of detecting the presence of a Botulinum toxin, comprising: providing an assay with a cell having (a) a molecule with a cleavage sequence that is cleaved by a light chain of said Botulinum toxin and (b) a reporter that reports a signal responsive to cleavage of said molecule at said cleavage sequence induced by the light chain of said Botulinum toxin; and incubating the cell in a culture media that contains a compound effective to increase sensitivity of the assay by at least 0.5 log; treating the cell with said Botulinum toxin, then detecting the signal, and correlating the signal with the presence or absence of said Botulinum toxin, wherein said compound is an isoquinolynyl compound that inhibits protein kinase C (PKC), and wherein said cleavage sequence comprises a SNARE protein, SNARE motif, or SNARE mutein.