Patent ID: 9404155
Filing Date: 2016-08-02
CPC Classification: C12Q,C40B,G16B

Claim Text:
1. A method for sequencing a polynucleotide in a preparation by a sequencing instrument, wherein the sequencing instrument comprises a processor, said method comprising: obtaining a polynucleotide preparation comprising the polynucleotide, comprising amplifying the polynucleotide in a semisolid support, determining, by the sequencing instrument, a first apparent sequence of a first region of a polynucleotide by subjecting a sample of the polynucleotide preparation to a first type of sequencing technique; simultaneously determining, by the sequencing instrument, a second apparent sequence of a second region of the polynucleotide that overlaps with the first region, by subjecting a sample of the same polynucleotide preparation to a second different type of sequencing technique, wherein the first and second sequencing techniques are selected from the group consisting of Maxam-Gilbert sequencing, chain termination methods, dye termination methods, sequencing using reversible terminators, sequencing of nucleic acid by pyrophosphate detection, sequencing by ligation, closed complex single molecule sequencing, nanoscale fluidic sequencing, and force spectroscopy platform sequencing; identifying, by the processor, any discrepancies between the first apparent sequence and the second apparent sequence; and selecting, by the processor, the least error prone sequence from the first apparent sequence and the second apparent sequence based upon the likelihood of an error or error rate in one sequencing technique compared to the other sequencing technique.