Patent ID: 9440980
Filing Date: 2016-09-13
CPC Classification: A61K,A61P,C07D,C12Q,G01N

Claim Text:
1. A method for screening a hypoglycemic compound which enhances enzymatic activity of phosphoinositide 3-kinase β (PI3-kinase β) comprising 1) reacting a Gβ protein which is selected from the group consisting of Gβ1 subtype, Gβ2 subtype, Gβ3 subtype, Gβ4 subtype, and Gβ5 subtype with PI3-kinase β and at least two substrates, 2) measuring activity of the PI3-kinase β by at least one method selected from the group consisting of: 3) repeating 1) and 2) in the presence of a test compound; 4) comparing the activity of the PI3-kinase β measured in 2) with the activity of the PI3-kinase β measured in 3); 5) determining whether the test compound has an effect of enhancing the enzymatic activity of PI3-kinase β wherein the activity in 3) is greater than the activity in 2); and 6) determining the hypoglycemic effect of the test compound which is found to enhance enzymatic activity of PI3-kinase β by administering the test compound to an animal and determining whether said test compound enhances the glucose uptake activity of a adipocyte.