Patent ID: 9422602
Filing Date: 2016-08-23
CPC Classification: C12Q,Y10T

Claim Text:
1. A method of detecting degradation of mRNA, the method comprising: (a) contacting a sample comprising mRNA with at least one agent to covalently attach an oligonucleotide label to a first end of RNA molecules having a first end structure and to covalently attach an oligonucleotide label to a second end of mRNA molecules having a second end structure; (b) separating the sample into a plurality of spatially isolated partitions; (c) enumerating a first number of spatially isolated partitions comprising an oligonucleotide label covalently attached to the first end of an mRNA molecule and an oligonucleotide label covalently attached to the second end of an mRNA molecule, and a second number of spatially isolated partitions comprising an oligonucleotide label covalently attached only to the first end or only to the second end of an mRNA molecule; and (d) determining an amount of degradation for the mRNA based on the enumerating, wherein the step of determining comprises comparing the first number and the second number to one another.