Patent ID: 9234894
Filing Date: 2016-01-12
CPC Classification: G01N,H01J

Claim Text:
1. A method for an analytical determination of Adenosine and Deoxyadenosine and a plurality of metabolites selected from the group consisting of purines, pyrimidines, amino acids, free carnitine, acylcarnitines and any combinations thereof from a dried blood sample, said method comprising: providing a dried blood sample; extracting Adenosine and Deoxyadenosine from the dried blood sample concurrently with the plurality of metabolites selected from the group consisting of purines, pyrimidines, amino acids, acylcarnitines, free carnitine, and any combinations thereof using an extraction mixture comprising a mixture of water and C1-3 linear or branched chain monoalcohol, wherein water is present at least at 10% v/v, wherein the extracting is performed by first contacting the dried blood sample with the C1-3 linear or branched chain monoalcohol corresponding to 100-x % v/v of the final volume v/v of the extraction mixture, and second adding water to the sample corresponding to x % v/v of the final volume v/v of the extraction mixture, wherein x is the v/v water percentage and ranges from about 10% to about 90%; and determining the presence or amount of Adenosine and Deoxyadenosine concurrently with the plurality of metabolites selected from the group consisting of purines, pyrimidines, amino acids, acylcarnitines, free carnitine, and any combinations thereof using MS directly on the extract.