Patent ID: 9376464
Filing Date: 2016-06-28
CPC Classification: C07K

Claim Text:
1. A method for purifying an IgG antibody produced in a microbial host cell, the method comprising the steps of: a. providing a mixture comprising an IgG antibody produced in a microbial host cell; b. adding one or more stimuli responsive polymers to the mixture under pH conditions such that the one or more polymers are solubilized and are capable of reversibly and selectively binding to the IgG antibody, said one or more polymers being one or more polyvinyl pyridine-containing polymers; c. mixing the one or more solubilized stimuli responsive polymers throughout the mixture; d. changing the pH conditions such that to precipitate the one or more polymers and bound IgG antibody out of solution; and e. separating the precipitated polymer and bound IgG antibody from the mixture.