Patent ID: 9487825
Filing Date: 2016-11-08
CPC Classification: C12N,C12Q

Claim Text:
1. A mixture of 3′ DNA adapters for small RNA characterization comprising: a plurality of modified adapter sequences that are covalently extended at the 5′ ends by addition of at least two random nucleotides, wherein the modified adapter sequences comprise the structure NNe, wherein e is the adaptor sequence, and wherein NN are at least two random nucleotides that have been covalently attached to the 5′ end of the adapter sequence; wherein at least one modified adapter sequence further comprises an activated riboadenylation (rAPP) at the 5′ end of the modified adapter sequence, wherein the at least two random nucleotides are internal to the activated riboadenylation (5′rAPP-NNe-3′); wherein the mixture comprises at least sixteen modified adapter sequences for each unique adapter sequence wherein the at least sixteen modified adapter sequences comprise AAe, ACe, AGe, ATe, CAe, CCe, CGe, CTe, GAe, GCe, GGe, GTe, TAe, TCe, TGe, and TTe; and wherein A is a deoxyadenylate nucleotide; C is a deoxycytidylate nucleotide; G is a deoxyguanylate nucleotide; and T is a thymidylate nucleotide.