Patent ID: 9290663
Filing Date: 2016-03-22
CPC Classification: C07D,C07H,C09B,C12Q

Claim Text:
1. A kit for amplifying and subsequently melting a plurality of target nucleic acids comprising: (a) deoxyribonucleotide triphosphates, (b) a polymerase, and (c) a dsDNA binding dye having a percent saturation of at least 90%, wherein the dye is operable to indicate the presence of heterozygous sequence alterations in the plurality of target nucleic acids and does not inhibit amplification of the plurality of target nucleic acids at 90% saturation, (d) a protocol for amplifying each of the plurality of target nucleic acids in the presence of the dsDNA binding dye using the deoxyribonucleotide triphosphates and the polymerase, to generate a plurality of amplified nucleic acids, and (e) a protocol for generating a fluorescence melting curve from each of the plurality of amplified nucleic acids and plotting the difference between fluorescence melting curves, wherein the kit is operable to distinguish a heterozygous genotype from a homozygous genotype or for scanning for heterozygous sequence alterations in the plurality of target nucleic acids.