Patent ID: 9309569
Filing Date: 2016-04-12
CPC Classification: C12Q

Claim Text:
1. A method for sequencing nucleic acids comprising, a) incorporating a nucleotide into one or more nucleic acids of a plurality of nucleic acids in one or more reaction chambers in contact with one or more ion detectors, wherein said nucleotides comprise a removable 3′-OH blocking group, said blocking group preventing any further nucleotide incorporation and any further extension of the nucleic acids in which the nucleotide is incorporated unless removed, wherein said nucleic acids comprise incorporated nucleotides in a chain and a portion of the sequence of said nucleic acids comprises consecutive identical bases of the formula X n , where X is any base and n is a whole number between 3 and 10, b) detecting hydrogen ions released upon said nucleotide incorporation by said one or more ion detectors, said hydrogen ions coming from the 3′-OH group of an already incorporated nucleotide in the chain when the new nucleotide is incorporated, and c) sequencing the nucleic acids by determining the identity of the incorporated nucleotide.