Patent ID: 9453205
Filing Date: 2016-09-27
CPC Classification: C12N

Claim Text:
1. An in vitro human neural multipotent or unipotent cell possessing all of the following characteristics: i) has the ability to proliferate for more than 50 population doublings, and has an average population doubling time of less than 3 days; ii) is not a cancerous cell; iii) is stable and not artificially maintained by forced gene expression and may be maintained in standard neural stem cell media; iv) can differentiate to a progenitor, precursor, or somatic cell; v) has the characteristics of a neural stem cell, neural precursor cell, neural progenitor cell or neuroblast; vi) does not exhibit uncontrolled growth, teratoma formation, and tumor formation in vivo; vii) expresses one or more markers of a multipotent, unipotent or somatic cell not characteristic of a neural stem cell, neural precursor cell, neural progenitor cell or neuroblast; viii) is capable of maintaining telomerase activity through to at least about 30 population doublings; ix) is derived from the reprogramming of a somatic cell, a progenitor cell or a stem cell that exhibits at least a transient increase in intracellular levels of at least one reprogramming agent; and x) wherein the cell comprises at least one polypeptide or an expression vector encoding at least one polypeptide selected from the group consisting of: Musashi1 (Msi1); Ngn2; Msi1 and Ngn2; Msi1 and methyl-CpG binding domain protein 2 (MBD2); Ngn2 and MBD2; Msi1, Ngn2 and MBD2; Achaete-Scute Homolog 1 (Ascl1); Msi1, Ngn2 and Ascl1; Msi1, Ngn2, MBD2 and Ascl1; Sox2; Msi1, Ngn2 and Sox2; and Msi1, Ngn2, MBD2 and Sox2; wherein the expression vector is transiently expressed.