Patent ID: 9249458
Filing Date: 2016-02-02
CPC Classification: C07H,C12Q

Claim Text:
1. A template-directed process for extending one of a plurality of primers by enzymatic polymerization, wherein the template is one of a plurality of oligonucleotides, wherein said process comprises (a) contacting in aqueous solution the said plurality of primers with one or more of said oligonucleotides, a polymerase, and standard nucleoside triphosphates, and (b) incubating the mixture for a preselected length of time, wherein each of said primers has the structure: wherein X is selected from the group consisting of OH, O-phosphate, O-oligonucleotide, —NH 2 , and a phosphate or an amino group linked to a biotin or a fluorescent tag, N is independently selected from the group consisting of adenine, thymine, guanine, cytosine, diaminopurine, uracil, A*, T*, G*, and C*, D is independently selected from the group consisting of A*, T*, G*, and C*, E is independently selected from the group consisting of A, T, G, and C, and F is independently selected from the group consisting of A, T, G, C, K, X, V, J, S, B, Z and P, wherein K, X, V, J, S, B, Z and P are the nucleobases disclosed in FIG. 1 or FIG. 2 , wherein at least one F is selected from the group K, X, V, J, S, B, Z and P, wherein n is an integer from 4 to 6, m is an integer from 10 to 20, and f is an integer from 5 to 30, wherein the sequence of said primer at its 3′-end is substantially complementary to a portion of the sequence of one of said oligonucleotides, where A* does not contribute to the stability of a duplex when paired with T* but does when it is paired with thymine, T* does not contribute to the stability of a duplex when paired with A* but does when it is paired with adenine, G* does not contribute to the stability of a duplex when paired with C* but does when it is paired with cytosine, and C* does not contribute to the stability of a duplex when paired with G* but does when it is paired with guanine.