Patent ID: 9493789
Filing Date: 2016-11-15
CPC Classification: A61K,C12N

Claim Text:
1. A process for generation of genetically modified stem cells comprising the steps: a) providing a cell sample in suspension comprising stem cells in a centrifugation chamber comprising a base plate and cover plate connected by a cylinder; b) adjusting the volumetric concentration of stem cells in the cell sample to at least 1×10 c) Introducing viral and/or non-viral vectors to the centrifugation chamber for genetically modifying the stem cells; and d) adjusting the spatial concentration of stem cells in the centrifugation chamber by rotating the centrifugation chamber at a speed where the cell sample is located at the outermost 35% of the radius of the base plate of the centrifugation chamber, thereby inducing gene modification of the stem cells.