Patent ID: 9511172
Filing Date: 2016-12-06
CPC Classification: A61L

Claim Text:
1. A method of manufacturing an allogeneic soft-tissue transplant having autologous stem cells transplanted therein, the method comprising: obtaining an allogeneic soft-tissue support from a body of a human or an animal; washing and sterilizing the obtained allogeneic soft-tissue support; removing cells involved in an immunological rejection response by treating the washed and sterilized allogeneic soft-tissue support with an enzyme solution containing a mixture of trypsin, collagenase and protease; forming at least one pore in the allogeneic soft-tissue support by applying a superfine fiber needle to the allogeneic soft-tissue support treated with the enzyme solution and from which the cells involved in the immunological rejection response are removed; injecting autologous stem cells of a recipient into the at least one pore in the allogeneic soft-tissue support before or at a time of injecting an autologous stem cell loading promoter; and incubating the allogeneic soft-tissue support, into which the autologous stem cells are injected, to obtain an allogeneic soft-tissue transplant having autologous stem cells transplanted therein, wherein the allogeneic soft-tissue support is incubated by providing a physical stimulus to the allogeneic soft-tissue support.