Patent ID: 9458454
Filing Date: 2016-10-04
CPC Classification: C12N,C12P

Claim Text:
1. An isolated viable Gram-negative bacterium with substantially reduced bacterial proteolytic activity, wherein said bacterium is Escherichia coli and comprises (a) a mutation in at least one of the genes selected from the group consisting of kdsA, kdsC, kdsB, and waaA, or a mutation in each of the kdsD and gutQ genes, wherein said mutation results in a disruption in the biosynthesis of (Kdo) 2 -lipid IV A , (b) a mutation in each of the genes pagP and eptA wherein said mutation substantially inactivates the enzymatic activity of each of the proteins encoded by the pagP and eptA genes, and (c) a suppressor mutation in the msbA gene or the yjhD gene, such that the outer membrane of the bacterium substantially lacks a ligand that acts as an agonist of TLR4/MD-2 signaling, and TLR4/MD-2 signaling is substantially abrogated.