Patent ID: 9441021
Filing Date: 2016-09-13
CPC Classification: A61K,A61P,C07K

Claim Text:
1. A process for the preparation of a monomeric, full-length hypoallergenic major birch pollen allergen rBet v 1 comprising chromatographic purification of a monomeric, full-length soluble rBet v 1 crude protein, said purification comprising employing an essentially unbuffered aqueous NaOH base in a concentration range from 5 mM to 40 mM as eluent; optionally adding NaHCO wherein said monomeric, full-length soluble rBet v 1 crude protein is prepared by recombinant methods and the chromatographic purification comprises a sequential (a) first gel filtration; (b) anion exchange chromatography; (c) hydrophobic interaction chromatography; and (d) second gel filtration.