Patent ID: 9243242
Filing Date: 2016-01-26
CPC Classification: C12N,C12Q

Claim Text:
1. A method of making a di-tagged single-stranded DNA from a double-stranded DNA, said method comprising the steps of: a) providing a double-stranded double-tagged oligonucleotide (DTO) sequentially comprising a sequence tag A, a breaking site, a sequence tag B, and a single nick or gap in one of the two strands; b) ligating two ends of said double-stranded DNA to said double-tagged oligonucleotide to generate a circular DNA-DTO; c) removing the nicked/gapped strand using an exonuclease to generate a circular single-stranded DNA; d) breaking said circular single-stranded DNA at said breaking site to generate a linear di-tagged single-stranded DNA.