Patent ID: 9423350
Filing Date: 2016-08-23
CPC Classification: G01N

Claim Text:
1. A method for determining a concentration (c) of a fluorescent substance in a medium, wherein an excitation radiation having an excitation wavelength (λ ex ) is radiated through the medium having the fluorescent substance so that the fluorescent substance is excited in such a way that the fluorescent substance emits a fluorescence radiation having a fluorescence wavelength (λ em ), wherein the intensity of the fluorescence radiation (I fluor ) is measured, from which the concentration (c) of the fluorescent substance in the medium is determined, characterised in that additionally the intensity (I trans,ex ) of a transmitted portion of the excitation radiation is measured, wherein the concentration (c) of the fluorescent substance is determined from the intensity of the transmitted portion of the excitation radiation (I trans,ex ) and the intensity of the fluorescence radiation (I fluor ); wherein furthermore a fluorescence absorption radiation having the fluorescence wavelength (λ wherein the intensity of the fluorescence radiation (I