Patent ID: 9410171
Filing Date: 2016-08-09
CPC Classification: B01L,C12P,C12Q

Claim Text:
1. A method for polymerase chain reaction (PCR) cycling, comprising: receiving an electrolytic fluid including ions, primers, polymerase enzymes, nucleotides, and a double-stranded nucleic acid in a fluid chamber having a first electrode and a second electrode located on two opposing sides of the electrolytic fluid; applying an electric field across the first and the second electrodes to generate a first pH level of the electrolytic fluid to denature the double-stranded nucleic acid to at least partial single strands; and applying a second electric field across the first and second electrodes to produce a second pH level of the electrolytic fluid, wherein the second pH level enables binding of a polymerase enzyme and a primer with a corresponding segment of the single strands.