Patent ID: 9310380
Filing Date: 2016-04-12
CPC Classification: G01N

Claim Text:
1. A method for assessing the presence of arteriosclerosis in a subject suspected of having arteriosclerosis, the method comprising: (a) obtaining a serum sample from the subject; (b) measuring an antibody titer of an autoantibody against an IL-5 polypeptide present in the sample, wherein the IL-5 polypeptide consists of a polypeptide sequence selected from the group consisting of SEQ ID NO:2 and SEQ ID NO:3, and wherein the IL-5 polypeptide is obtained by Cell Free Protein Synthesis System using wheat germ; and (c) comparing the antibody titer of the autoantibody against IL-5 in the serum from the subject with an antibody titer of an autoantibody against IL-5 in a serum sample from a healthy subject; wherein a significant increase in the titer of the autoantibody against IL-5 in the serum sample from the subject as compared to the titer of the autoantibody against IL-5 in the serum sample from the healthy subject is indicative of the presence of arteriosclerosis in the subject.