Patent ID: 9488637
Filing Date: 2016-11-08
CPC Classification: A61N,C12M,C12N,G01N

Claim Text:
1. A method for stimulating and measuring exocytosis from a cell, comprising: applying at least one voltage pulse to a cell that is in the presence of an exogenous inorganic anion and in the absence of exogenous free calcium ions through an electrode on a device to provide a voltage gradient in solution surrounding the cell, said cell residing in a drop of solution such that said cell is adjacent to said electrode, said solution drop comprising said exogenous inorganic anion, prepared without addition of a calcium ion source compound, and being confined to a part on the device where the electrode is located such that said solution drop is sitting on a surface of said electrode, the applied voltage and concentration of said inorganic anion being sufficient to cause a stimulation of said cell; and, recording, through said same electrode while the cell remains adjacent to the electrode, a current signal indicative of a release of an electrochemically active substance from said stimulated cell.