Patent ID: 9301544
Filing Date: 2016-04-05
CPC Classification: A23L,A24B,A61K,A61Q,C07K

Claim Text:
1. A method for obtaining a protein-enriched material from a plant of the Nicotiana species or portion thereof, comprising: receiving a plant material of the contacting the plant material with a solvent for a time and under conditions sufficient to extract one or more proteins from the plant material into the solvent and form a liquid protein-containing extract; separating a solid extracted plant material from the liquid protein-containing extract; clarifying the liquid protein-containing extract to form a clarified protein-containing extract and a solids fraction; and treating the clarified protein-containing extract so as to provide a protein-enriched material comprising at least 60% protein by dry weight, wherein the treating step comprises adjusting the pH of the clarified protein-containing extract to a pH of between about 3 to about 4.5; isolating a precipitate from the acidic extract; and washing the precipitate to provide a RuBisCO-containing and F2 protein-containing protein-enriched material.