Patent ID: 9404857
Filing Date: 2016-08-02
CPC Classification: G01N

Claim Text:
1. A method for obtaining a tomographic phase image of a specimen, the method comprising: a. illuminating the specimen with substantially temporally incoherent light through an objective of high-numerical aperture, the temporally incoherent light characterized by a focus; b. stepping the focus of the temporally incoherent light to a sequence of successive focal planes within the specimen; c. forming a scattered phase image of the specimen in an image plane where the scattered phase image of the specimen corresponds to each of the successive focal planes; d. solving for the scattered field in wavevector space; e. deconvolving a derived instrument function to obtain specimen susceptibility in wavevector space; and f. transforming the specimen susceptibility to derive a three-dimensional phase tomogram of the specimen.