Patent ID: 9469861
Filing Date: 2016-10-18
CPC Classification: A61P,C12N,C12P,C12Y,Y02P

Claim Text:
1. A method of generating a compound of Formula (I-a): or a salt or tautomer thereof, or a combination thereof; the method comprising: (1) providing one or more cell lysates comprising: or isozymes thereof; wherein the one or more cell lysates are contacted with glucose and glycine, or salts thereof, to provide a compound of Formula (I-a) or a salt or tautomer thereof or a combination thereof, wherein R (2) providing one or more cell lysates comprising: or isozymes thereof; wherein the one or more cell lysates are contacted with glucose and glycine, or salts thereof, to provide a compound of Formula (I-a) or a salt or tautomer thereof or a combination thereof, wherein R (3) providing one or more cell lysates comprising: or isozymes thereof; wherein the one or more cell lysates are contacted with glucose and an optionally substituted glutamate of the formula (i): or salts thereof, to provide a compound of Formula (I-a) or a salt or tautomer thereof or a combination thereof, wherein R 1 is hydrogen and R 2 is —CH 2 CH 3 ; or (4) providing one or more cell lysates comprising: or isozymes thereof; wherein the one or more cell lysates are contacted with glucose and an optionally substituted glutamate of the formula (i): or salts thereof, to provide a compound of Formula (I-a) or a salt or tautomer thereof or a combination thereof, wherein R 1 is hydrogen and R 2 is hydrogen; or (5) providing one or more cell lysates comprising: or isozymes thereof; wherein the one or more cell lysates are contacted with glucose and a proline compound of formula (iv): or salts thereof, wherein R 5 is hydrogen, to provide a compound of Formula (I-a) or a salt or tautomer thereof or a combination thereof, wherein R 1 is hydrogen, R 2 is —CH 2 CH 3 , and R 5 is —OH; or (6) providing one or more cell lysates comprising: or isozymes thereof; wherein the one or more cell lysates are contacted with glucose and a proline compound of formula (iv): or salts thereof, wherein R 5 is hydrogen, to provide a compound of Formula (I-a) or a salt or tautomer thereof or a combination thereof, wherein R 1 is hydrogen, R 2 is hydrogen, and R 5 is —OH; or (7) providing one or more cell lysates comprising: or isozymes thereof; wherein the one or more cell lysates are contacted with glucose and a proline compound of formula (iv): or salts thereof, to provide a compound of Formula (I-a) or a salt or tautomer thereof or a combination thereof, wherein R 1 is hydrogen and R 2 is —CH 2 CH 3 ; or (8) providing one or more cell lysates comprising: or isozymes thereof; wherein the one or more cell lysates are contacted with glucose and a proline compound of formula (iv): or salts thereof, to provide a compound of Formula (I-a) or a salt or tautomer thereof or a combination thereof, wherein R 1 is hydrogen and R 2 is hydrogen; wherein: the dashed line each instance of R R each instance of R R wherein one or more enzymes selected from the group consisting of carboxymethyl-Pro synthase, carbapenam synthetase, beta-lactam synthetase, carbapenem synthase, and isozymes thereof, is sequestered in the periplasmic space of the cell prior to lysing; and wherein the one or more cell lysates are bacterial cell lysates.