Patent ID: 9476888
Filing Date: 2016-10-25
CPC Classification: C07K,G01N

Claim Text:
1. A method of detecting a ubiquitinated site in a sample protein or a mixture of sample proteins that comprises (i) cleaving a sample protein or a mixture of sample proteins with a protease comprising LysC that cleaves ubiquitin to form a mixture of cleavage peptides, wherein the protease comprises LysC that cleaves ubiquitin also cleaves protein(s) in the sample or the mixture of sample protein; (ii) contacting the cleavage peptides with an antibody that specifically binds to an epitope having the sequence ESTLHLVLRLRGG (SEQ ID NO 1), said antibody being able to specifically distinguish between proteins and peptides that are ubiquitinated and proteins and peptides that are modified by ubiquitin-like modifiers; (iii) analysing the peptide(s) that bind to the antibody thereby detecting and identifying an ubiquitinated site in the test sample protein of the mixture of the sample proteins; (iv) sequencing the cleavage peptide to which the antibody binds to thereby determine the amino acids sequence of the cleavage peptide; and (v) identifying the ubiquitinated site in the cleavage peptide,