Patent ID: 9464294
Filing Date: 2016-10-11
CPC Classification: C12N

Claim Text:
1. A method for producing a heterologous polypeptide in a recombinant bacterial host cell, which is subject to carbon catabolite repression and a phosphoenolpyruvate: carbohydrate phosphotransferase system and which is genetically altered such, that it is incapable of deactivating a transcriptional regulator protein ManR specific for a promoter inducible by a secondary carbon source mannose and which comprises a vector with a heterologous nucleic acid sequence encoding a polypeptide operably linked to the mannose-inducible promoter, said method comprising the steps of growing the bacterial host cell in a cell culture medium containing only a primary carbon source glucose but not the inducer mannose, whereby the expression of said polypeptide is not induced by mannose but by allowing the concentration of glucose to decrease during the duration of the a cell culture below a level that causes carbon catabolite repression, and recovering the polypeptide from the bacterial host cell or from the cell culture medium.