Patent ID: 9480982
Filing Date: 2016-11-01
CPC Classification: A61F,B01L,G02C

Claim Text:
1. A reactor for the quantitative analysis of target nucleic acids, comprising: a thermally conductive polypropylene substrate having a first planar opposing surface, and a cavity; a buffer layer comprising water-impermeable sealant arranged over and adhered to the first planar opposing surface of the substrate; a K9 optical glass cover plate with probes tethered to the interior surface of the cover plate in a known two-dimensional pattern, where the cover plate is arranged over the buffer layer, the cover plate in combination with the cavity, the first planar opposing surface, and buffer layer defining a reaction chamber; and at least one inlet port and at least one outlet port communicating with the reaction chamber through the substrate enabling the passage of fluid comprising a sample to be analyzed, analytical reagents, and/or reactants from an external source into and through the reaction chamber, and thereby defining a fluid flow path; wherein the reactor is configured for quantitative analysis of target nucleic acids using a polymerase chain reaction (PCR) process and an evanescent wave detection technique where the evanescent wave enters the reaction chamber through the cover plate to which the target nucleic acid probes are tethered.