Patent ID: 9494666
Filing Date: 2016-11-15
CPC Classification: G01R,G06F,G16B

Claim Text:
1. A method of measuring ionized constituents in a biosample, comprising: transforming a biosample by adding EDTA to the biosample; subjecting, via a nucleic magnetic resonance (NMR) spectrometer, the transformed biosample to single-pulse NMR, the obtained NMR signal data including: generating an NMR spectrum based on the electronically obtained NMR signal data, the NMR spectrum including: deconvolving, using one or more processors, at least part of the NMR spectrum, the at least part of the NMR spectrum including: the first spectrum data corresponding to the ethylenic proton singlet region for ionized calcium; and the second data corresponding to the ethylenic proton singlet region for ionized magnesium, wherein the deconvolving resolves at least one of the ionized calcium or ionized magnesium ethylenic proton singlet regions from background due to lipoproteins in the biosample; identifying, using the one or more processors, a single peak for each of one or both of the ionized calcium and the ionized magnesium based on the deconvolution; calculating, using the one or more processors, at least one of: generating an electronic report that includes the at least one of the quantitative concentration of ionized calcium or the quantitative concentration of the ionized magnesium, wherein the NMR spectrometer is a 400 MHz spectrometer; first signal data corresponding to the ethylenic proton singlet region for ionized calcium is centered at 2.52 ppm and the second signal data corresponding to the ethylenic proton singlet region for ionized magnesium is centered at 2.66 ppm; the calculating the at least one of the quantitative concentration of ionized calcium and the quantitative concentration of ionized magnesium includes: using a relationship for determining the quantitative concentration of ionized calcium that is linear up to about 6.0 mM and has a within run and total precision with a coefficient of variation (% cv) that is less than 3%; or using a relationship for determining the quantitative concentration of ionized magnesium that is linear up to about 4.0 mM and has a within run and total precision with a coefficient of variation (% cv) that is less than 4%.