Patent ID: 9238843
Filing Date: 2016-01-19
CPC Classification: C12Q

Claim Text:
1. A method for detecting the presence of a BVDV-1b live virus, the method comprising: diluting a sample in (i) an assay plate containing cultured bovine cells in which live virus particles are capable of replicating and (ii) a corresponding reference plate without cells; contacting nucleic acids from at least one sample dilution with a BVDV-1b virus-specific primer/probe set, said virus-specific primer/probe set comprising a forward BVDV-1b virus-specific oligonucleotide amplification primer, a reverse BVDV-1b virus-specific oligonucleotide amplification primer, and a BVDV-1b virus-specific oligonucleotide detection probe; and performing at least one cycling step, wherein the at least one cycling step is performed using quantitative real-time polymerase chain reaction (qPCR); wherein said BVDV-1b virus-specific primer/probe set is useful in detecting the presence of a BVDV-1b viral-specific polynucleotide in a population of polynucleotides obtained from a plurality of bovine virus particles; wherein said plurality of bovine virus particles comprises at least one BVDV Type 1b virus particle; and wherein a higher level of a signal from a virus-specific oligonucleotide detection probe in the presence of nucleic acids from the assay plate compared to a signal from the virus-specific oligonucleotide detection probe in the presence of nucleic acids from the reference plate indicates the presence of a live bovine virus in the sample.