Patent ID: 9359641
Filing Date: 2016-06-07
CPC Classification: C12Q,G06K,G06T

Claim Text:
1. A method for use in performing biochemical experiments comprising: providing a microarray comprising a solid planar substrate having a surface; preparing a substrate for imaging to establish location and registration of biochemical sites on the substrate by providing a predetermined two-dimensional spatial pattern of biochemical sites in periodic distance between adjacent sites on the substrate; detecting image field spots in a spatial two-dimensional array on the substrate corresponding to biochemical sites in an image on the substrate; aligning a spatial two-dimensional grid with image field spots of biochemical sites on the substrate to determine a grid offset in order to permit alignment using Moiré patterns; the surface further comprising a regular two-dimensional array of optically resolvable surface sites, the optically resolvable surface sites comprising: (a) attachment sites configured for attachment of biomolecules and (b) sites in a plurality of preselected deletion patterns configured to prevent attachment of the biomolecules, wherein each deletion pattern is configured to cross-correlate to at least one mask pattern, capturing an image of the microarray; registering the image comprising cross correlating the deletion patterns to the mask pattern to determine absolute location within a field.