Patent ID: 9428767
Filing Date: 2016-08-30
CPC Classification: A61K,C07K,C12N,C12Y

Claim Text:
1. A polynucleotide comprising a transposon comprising inverted repeats of a piggyBac-like transposon flanking a heterologous polynucleotide, the inverted repeats being flanked by copies of the target site 5′-TTAT-3′ at each end, such that the transposon can be excised leaving a single copy of the 5′-TTAT-3′ target site in place of the transposon in the polynucleotide, wherein the transposon comprises at least 16 contiguous nucleotides from SEQ ID NO: 1 to provide a copy of the 5′TTAT-3′ target site and one inverted repeat and at least 16 contiguous nucleotides from SEQ. ID NO: 2 to provide the other copy of the 5′-TTAT-3′ target site and the other inverted repeat.