Patent ID: 9492482
Filing Date: 2016-11-15
CPC Classification: A61K,A61P,C12N

Claim Text:
1. An in vitro engineered immune cell comprising an adenoviral vector for conditionally expressing proteins, the vector comprising a polynucleotide encoding an ecdysone gene switch, wherein said polynucleotide comprises (1) a polynucleotide sequence comprising a first transcription factor sequence and a second transcription factor sequence under the control of a promoter, wherein the proteins encoded by the first transcription factor sequence and second transcription factor sequence interact to form a ligand-dependent transcription factor complex, (2) a polynucleotide encoding a polypeptide at least 90% identical to IL-12; and (3) a polynucleotide encoding a polypeptide at least 90% identical to IL-21; wherein at least one polynucleotide of (2) or (3) are linked to a promoter capable of being activated by said ligand dependent transcription factor complex.