Patent ID: 9499851
Filing Date: 2016-11-22
CPC Classification: C12Q,G01N

Claim Text:
1. A method for detecting bell-shaped nuclei in metakaryotic stem cells in a subject, the method comprising: a) obtaining a vascular tissue sample from the subject suspected of having neointimal hyperplasia or restenosis; b) partially dissociating the tissue sample by macerating and spreading the tissue sample by a method that substantially preserves the integrity of bell-shaped nuclei of metakaryotic stem cells in the tissue sample, the nuclei having maximum diameters from about 10-50 microns; c) fixing and staining the tissue sample to visualize the bell-shaped nuclei of the metakaryotic stem cells; d) detecting the presence and/or absence of metakaryotic stem cells in the subject tissue sample and in a control tissue sample.