Patent ID: 9309560
Filing Date: 2016-04-12
CPC Classification: C12P,C12Q

Claim Text:
1. A method for preparing a plurality of nucleic acids, comprising: a) producing a plurality of circular nucleic acids, by intramolecularly ligating a plurality of nucleic acid fragments each having one or more first adaptors joined to the 5′ and 3′ ends of a linear nucleic acid fragment; b) fragmenting the plurality of circularized nucleic acids by shearing, thereby producing a plurality of linear paired tag fragments containing the one or more first adaptors and a paired tag having the 5′ end and the 3′ end of the linear nucleic acid fragment in step (a); and c) joining a second adapter to both ends of the plurality of linear paired tag fragments thereby producing a plurality of adaptor-joined paired tag fragments.