Patent ID: 9494595
Filing Date: 2016-11-15
CPC Classification: C12N,G01N

Claim Text:
1. A method for detecting an interaction between a first protein and a second protein, the method comprising the steps of: expressing in a cell a first fusion protein comprising the first protein and an association-inducing protein, and a second fusion protein comprising the second protein and a fluorescent protein having a multimerization ability and able of emitting fluorescence; determining that there exists an interaction between the first protein and the second protein by detecting a fluorescent focus, wherein the association-inducing protein is at least one protein selected from the group consisting of PB1 domain of p62, a PB1 domain of TFG, a PB1 domain of PKCiota, a SAM domain of TEL, a SAM domain of DGK delta, and a SAM domain of Tankyrase-1; the fluorescent protein having a multimerization ability is at least one fluorescent protein selected from the group consisting of a fluorescent protein capable of forming a homomultimer, monomeric Kusabira-Orange 2, monomeric Keima-Red, monomeric Midoriishi-Cyan1, monomeric Kusabira-Orange 1, and monomeric Kikume Green-Red1; and the fluorescent focus has a fluorescence intensity in a region of 0.2 to 5 μm, the fluorescence intensity being higher than a fluorescence intensity of the fluorescent protein which is present in a dispersed state in the cell, and wherein the association inducing protein is able to form a fluorescent focus when fused to the fluorescent protein having an multimerization ability.