Patent ID: 9518295
Filing Date: 2016-12-13
CPC Classification: C12N,C12Q

Claim Text:
1. A method for constructing a methylated DNA library, consisting of: A) fragmenting a DNA sample; B) ligating auxiliary adapters to the ends of the fragmented DNA obtained in A); C) enriching methylated DNA fragments; D) removing fragments comprising moderately and highly repetitive sequences from the DNA fragments obtained in C); E) converting unmethylated cytosines in the product obtained in D) into uracils by bisulfite treatment; F) amplifying the DNA obtained in E) with primers; and G) removing the auxiliary adapters by digesting the product obtained in step F) with a restriction enzyme, a) an adapter that is free of restriction enzyme cutting sites and has a non-joining end that is an overhang structure and a joining end that is a blunt end; b) an adapter that is free of restriction enzyme cutting sites and has a non-joining end that is a forked structure and a joining end that is a blunt end; c) an adapter that is free of restriction enzyme cutting sites and has a non-joining end that is an overhang structure and a joining end that is a sticky end; or d) an adapter that is free of restriction enzyme cutting sites and has a non-joining end that is a forked structure and a joining end that is a sticky end;