Patent ID: 9382519
Filing Date: 2016-07-05
CPC Classification: C12N,C12P,C12Y,Y02P

Claim Text:
1. An engineered ketoreductase polypeptide capable of converting the substrate, methyl-2-benzamidomethyl-3-oxobutyrate, to the product, 2S,3R-methyl-2-benzamidomethyl-3-hydroxybutyrate, with a percent stereomeric excess of at least 60%, wherein said ketoreductase polypeptide comprises an amino acid sequence that is at least 85% identical to SEQ ID NO: 87, wherein the engineered ketoreductase polypeptide comprises the following features: the residue corresponding to X94 is threonine; the residue corresponding to X199 is a histidine, alanine, or asparagine, and the residue corresponding to X202 is valine or leucine.