Patent ID: 9290794
Filing Date: 2016-03-22
CPC Classification: C12N,C12Q

Claim Text:
1. A polynucleotide encoding a circularly-permuted thermostable luciferase comprising a peptide linker linking the original N- and C-termini of the non-permuted thermostable luciferase, wherein the peptide linker comprises a granzyme B recognition site, wherein the circularly-permuted thermostable luciferase has increased luminescence following cleavage by granzyme B when compared to an uncleaved circularly-permuted thermostable luciferase, wherein the granzyme B recognition site is selected from the group consisting of GRIEADSE (SEQ ID NO:80), KSVGPDFG (SEQ ID NO:81), and GIETDSG (SEQ ID NO:82), and wherein the non-permuted thermostable luciferase has at least 90% identity to the amino acid sequence of SEQ ID NO:2 or at least 90% identity to the amino acid sequence of SEQ ID NO:4.