Patent ID: 9249392
Filing Date: 2016-02-02
CPC Classification: A61P,C12N

Claim Text:
1. A method for culturing cardiac stem cells for use in the repair or regeneration of cardiac tissue while reducing DNA damage during culturing, the method comprising: isolating cardiac stem cells, wherein said cardiac stem cells are isolated from healthy mammalian non-embryonic cardiac tissue; and culturing said isolated cardiac stem cells as a monolayer culture in a culture media comprising an antioxidant composition, said antioxidant composition comprising glutathione present in a concentration ranging from 5 to 20 μM, vitamin C present in a concentration ranging from 0.1 to 20 μM, and vitamin E present in a concentration ranging from 0.1 to 20 μM, wherein said antioxidant composition present in said culture media is sufficient to (i) reduce reactive oxygen species to a level which decreases oxidative stress induced DNA damage, (ii) not reduce markers of DNA repair mechanisms to a statistically significant degree, and (iii) not induce markers of DNA damage in said cardiac stem cells to a statistically significant degree, when compared to cardiac stem cells not contacted with said antioxidant composition.