Patent ID: 9290752
Filing Date: 2016-03-22
CPC Classification: A61P,C07K,C12N

Claim Text:
1. A method of purifying alpha-amylase inhibitor protein from a Phaseolus vulgaris bean extract material, comprising the steps of: (a) suspending a bean extract material derived from a (b) adding a preservative to the solution; (c) heating the solution to about 175° F. to about 195° F., for from about 10 to about 120 seconds; (d) cooling the solution to a temperature of from about 65° F. to about 85° F. and letting the solution sit, under static non-agitated conditions, at a temperature within that defined range for from about 3 to about 24 hours to allow bean extract particles formed therein to settle out of the solution; and (e) separating the particles from the solution to obtain a supernatant, wherein the supernatant contains the alpha-amylase inhibitor protein.