Patent ID: 9435805
Filing Date: 2016-09-06
CPC Classification: C12Q,G01N

Claim Text:
1. A device for detecting the presence or absence of a bacterium in a sample, comprising: (a) a reaction chamber configured to hold the sample and comprising a first amplifier configured to release a substrate in response to an enzyme produced and/or secreted by the bacterium; (b) a membrane; (c) a second amplifier in fluid communication with the first amplifier, configured to bind to the released substrate and to produce a signal in the presence of the bacterium; and (d) a lateral flow cassette comprising a conjugate pad in fluid communication with the membrane, a lateral flow strip, and a wicking pad; wherein the membrane is configured to allow the sample to flow to the conjugate pad and lateral flow strip under conditions that allow the released substrate to bind to the second amplifier, wherein the membrane or conjugate pad filters the first amplifier away from the released substrate such that the first amplifier is prevented from entering the lateral flow strip, and wherein the lateral flow strip is configured to indicate the signal in the presence of the bacterium.