Patent ID: 9289502
Filing Date: 2016-03-22
CPC Classification: A61K,C07H,C07K,Y10S

Claim Text:
1. A method of preparing a compound of the formula comprising (i) attaching a conjugation component of the formula wherein R 2 is R 2a or R 2ap , to a solid support to form a compound of the formula (ii) when R 2 is R 2ap , converting (iii) contacting with a conjugation component of the formula wherein R 3 is R 3a , or R 3ap ; to form (iv) when R 3 is R 3ap , converting to and (v) contacting with a conjugation component of the formula to provide the compound of the formula wherein: is a solid support material; are independently an oligo, where each oligo is independently a polymer, having from 2 to about 150 covalently linked monomer units, that is characterized by a sequence of 2′-deoxyribosenucleotide residues (DNA), ribonucleotide residues (RNA), and peptide nucleic acids (PNAs), or a combination thereof; R (a) R or (b) R (c) R (d) R (e) R (f) R (g) R (h) R (i) R R 1b is thio, and L 1 is or (j) R or (k) R (l) R (m) R (n) R (o) R (p) R (q) R (r) R (s) R (t) R and L 1 is (u) R R (a′) R or (b′) R (c′) R (d′) R (e′) R (f) R (g′) R (h′) R (i′) R R 2b is thio, and L 2 is or (j′) R or (k′) R (l′) R (m′) R (n′) R (o′) R (p′) R (q′) R (r′) R (s′) R (t′) R and L 2 is or (u′) R R (a″) R or (b″) R (c″) R (d″) R (e″) R (f″) R (g″) R (h″) R (i″) R R 3b is thio, and L 3 is or (j″) R or (k″) R (l″) R (m″) R (n″) R (o″) R (p″) R (q″) R (r″) R (s″) R (t″) R and L 3 is or (u″) R R X is chlorine, bromine, fluorine, tosylate, mesylate, triflate, or dimethoxy triflate, n is 1, 2, 3, 4, 5, or 6; R Pr is an amino protecting group; R R n is 1, 2, 3, 4, 5, or 6; s is an integer of greater than 1; provided that R - - - represents the point of connection to the part of the solid support-bound conjugated molecule that is closer to and represents the point of connection to the part of the solid support-bound conjugated molecule that is further away from