Patent ID: 9404867
Filing Date: 2016-08-02
CPC Classification: G01N,G02B

Claim Text:
1. A luminescence microscopy method comprising: providing a sample having a sample surface and including a specific substance, wherein the specific substance can be repeatedly transformed from a first state in which it can be excited to emit luminescence radiation into a second state in which it cannot be excited to emit luminescence radiation; transforming the specific substance present in the sample from the second state into the first state by irradiation of switching radiation; and exciting the specific substance present in the sample to emit luminescence radiation by irradiation of excitation radiation and imaging the sample emitting luminescence radiation; wherein one or more sample areas extending perpendicular to the sample surface are selected with high resolution by irradiating one of the switching radiation and the excitation radiation as structured sample illumination being structured in a plane parallel to the sample surface, and wherein the one or more sample areas extending parallel to the sample surface selected with high resolution by irradiating the other one of the switching radiation and the excitation radiation as TIRF illumination of the sample.