Patent ID: 9238831
Filing Date: 2016-01-19
CPC Classification: C12Q

Claim Text:
1. A method for quantifying nucleic acids comprising: a) labelling a universal reference oligonucleotide having a predetermined length with a detectable marker; b) generating a standard curve using serial dilutions of the labelled universal reference oligonucleotide by plotting the intensity of the detectable marker against concentration of labelled universal reference oligonucleotide; c) amplifying a target nucleic acid in the presence of the detectable marker which labels the amplified target nucleic acid, d) comparing in real time the intensity of the detectable marker associated with the labelled amplified target nucleic acid, with the standard curve and determining the quantity of the amplified target nucleic acid, wherein said labelled universal reference oligonucleotide is not amplified during generation of the standard curve.