Patent ID: 9228206
Filing Date: 2016-01-05
CPC Classification: C12N

Claim Text:
1. A method of transferring a foreign gene into a target cell using a retroviral vector, the method comprising the following steps (a) to (e): (a) immobilizing a retrovirus-binding substance on an inner surface of a bag for cell culture; (b) placing a liquid containing a retroviral vector carrying a foreign gene in the bag obtained by step (a), followed by incubation with shaking at a temperature of less than 25° C. for 8 to 48 hours to obtain the bag to which the retroviral vector is bound; (c) adding a target cell into the bag obtained by the step (b), followed by incubation for 1 to 4 hours; (d) turning the bag obtained by step (c) upside down; and then (e) incubating the bag obtained by step (d) while upside down.