Patent ID: 9434924
Filing Date: 2016-09-06
CPC Classification: C12M,C12N

Claim Text:
1. A cell culture method of culturing adherent cells, comprising: holding a liquid culture medium in a culture bag which is formed by shaping at least one sheet having flexibility and permeability into a bag shape, the sheet having an inner cell-contacting surface having hydrophilicity sufficient to promote a culture of cells that adhere to the inner cell-contacting surface, whereby upon sowing, the cells adhere directly to the inner cell-contacting surface having hydrophilicity; incubating the cells; discharging the culture medium from the culture bag, and then filling the culture bag with a dissociation solution which does not contain trypsin, the dissociation solution containing ornithine or a derivative of ornithine selected from N-[amino(hydroxyimino)methyl]-ornithine or a structural isomer thereof, N-acetyl-ornithine, N-acyl-ornithine, N-(L-carboxyethyl)-ornithine, N-succinyl-ornithine, N,N-dibenzoyl-ornithine, (difluoromethyl)ornithine and N-[(hydroxy) (imino)methyl]-ornithine, the dissociation solution filling the culture bag through a solution sending circuit which connects the culture bag and a bag holding the dissociation solution to each other; bending the at least one sheet of the culture bag filled with the dissociation solution to dissociate the adherent cells from the inner cell-contacting surface; and collecting the adherent cells dissociated from the inner cell-contacting surface.