Patent ID: 9289742
Filing Date: 2016-03-22
CPC Classification: B01J,C07K,C12N,C40B

Claim Text:
1. A method of producing a protein multimer-nucleic acid complex comprising the protein multimer and a nucleic acid encoding any one target component of the protein multimer, which comprises the following steps: i) translating a nucleic acid encoding the target component into the target component by an in vitro translation system to give a translation product containing a target component-nucleic acid complex containing the target component and the nucleic acid encoding the target component, and ii) translating a nucleic acid encoding a non-target component constituting the protein multimer together with the target component into the non-target component by adding the nucleic acid encoding the non-target component to the translation product obtained in i) to give the non-target component, associating the non-target component with the target component in the target component-nucleic acid complex to form the protein multimer, thus affording the protein multimer-nucleic acid complex comprising the protein multimer and the nucleic acid encoding the target component, wherein the target component is a component of interest among components constituting the protein multimer, and the non-target component is a component other than the target component, constituting the protein multimer together with the target component.