Patent ID: 9458433
Filing Date: 2016-10-04
CPC Classification: A61K,A61P,C07D,C12N

Claim Text:
1. A method for preparing dense bodies, comprising (1) preparing isolated biological cells that are infected with human cytomegalovirus (HCMV) in a medium, and (2) incubating the infected biological cells with one of the following substances (A), (B), (C) or (D) at a concentration of the substance (A), (B), (C) or (D) of approximately 1 nmol/l to approximately 1 μmol/l: substance (A), which is (4S)-{8-fluoro-2-[4-(3-methoxyphenyl)-1-piperazinyl]-3-[2-methoxy-5-(trifluoromethyl)-phenyl]-3,4-dihydro-4-quinazolinyl}acetic acid; substance (B), which is N-{1-methyl-2-[(4-(5-methyl-pyridin-2-yl)-piperazin-1-yl)carbonyl]-1H-imidazol-4-yl}-N′-[4-(trifluoromethoxy)phenyl]urea; substance (C), which is 1-[6-fluoro-8-methoxy-3-({[2-methyl-4-(trifluoromethoxy)benzyl]amino}carbonyl)-4-oxo-1-(2,2,2-trifluoroethyl)-1,4-dihydroquinolin-7-yl]piperidine-4-carboxylic acid; or substance (D), which is N-{3-[({4-[5-(6-aminopyridin-2yl)-1,2,4-oxadiazol-3-yl]phenyl}sulfonyl)amino]-5-fluorophenyl}-1-cyanocyclopropane carboxamide, and then (3) isolating the resultant dense bodies from the cell supernatant without lysing the cells.