Patent ID: 9289533
Filing Date: 2016-03-22
CPC Classification: A61L

Claim Text:
1. A method for making a solid three-dimensional scaffold for cell therapy, tissue engineering and medico-surgical devices in the thoracic and surgical field, comprising: providing a scaffold support comprising a cross-linked collagen matrix, the collagen matrix being a collagen sponge and the collagen being purified from collagen-containing tissue; further crosslinking the collagen matrix; and modifying the scaffold support by covalently binding adhesion molecules to the collagen, wherein the adhesion molecules are RGD-containing peptides, said binding comprising covalently and uni-directionally binding primary amine groups present in the adhesion molecules to primary amine groups present in the collagen utilizing the heterobifunctional coupling reagent sulfosuccinimidyl 6-(3′-[2-pyridyldithio]-propionamido)hexanoate (Sulfo-LC-SPDP), said binding being performed by reacting the collagen with Sulfo-LC-SPDP, wherein the Sulfo-LC-SPDP covalently binds to a primary amine group of the collagen; reacting the adhesion molecules with Sulfo-LC-SPDP, wherein the Sulfo-LC-SPDP covalently binds to a primary amine group of the adhesion molecule; and reacting the Sulfo-LC-SPDP covalently bound to the collagen with the Sulfo-LC-SPDP covalently bound to the adhesion molecule, the Sulfo-LC-SPDP coupling reagents covalently binding together to form a disulfide bond, wherein the RGD-modified collagen matrix allows survival and differentiation of implanted contractile or potential contractile cells and forms a spontaneously contractile three dimensional scaffold.