Patent ID: 9266934
Filing Date: 2016-02-23
CPC Classification: C07K,C12N,C12P,C12Y,G06Q

Claim Text:
1. A method for producing a target peptide, the method comprising: expressing a soluble heterologous fusion peptide in a genetically modified cell, the soluble heterologous fusion peptide comprising an affinity tag, a cleavable tag, and the target peptide, wherein the cleavable tag is tryptophan (Trp), and wherein the affinity tag or the cleavable tag is heterologous to the target peptide; binding the soluble heterologous fusion peptide to an affinity material via the affinity tag; and cleaving the soluble heterologous fusion peptide with N-chlorosuccinimide while bound to the affinity material to release the target peptide, thereby producing the target peptide.