Patent ID: 9365816
Filing Date: 2016-06-14
CPC Classification: C12M,C12N

Claim Text:
1. A mechanical cell lysing apparatus with single cell resolution, comprising: an inlet port having an inlet valve configured to permit a source of biological material having a cell of interest to enter said mechanical single cell lysing apparatus; a drain port having a drain valve configured to permit said source of biological material to exit said mechanical single cell lysing apparatus; a trap structure configured to trap said cell of interest in said single cell lysing apparatus; the trap structure having an inlet and an outlet; a stress raiser configured to raise a mechanical stress on a membrane of said cell of interest trapped in said trap structure, wherein the stress raiser is positioned within the outlet of the trap structure; a pressure application element having a pressure application valve configured to apply a pressure to said cell of interest, said pressure application element configured to cooperate with said stress raiser to provide sufficient mechanical stress to lyse said cell of interest trapped in said trap structure; and an outlet port having an outlet valve configured to allow components of said lysed cell of interest to exit said mechanical single cell lysing apparatus to be analyzed.