Patent ID: 9347106
Filing Date: 2016-05-24
CPC Classification: C12Q

Claim Text:
1. A method for detecting the presence or absence of nine citrus pathogens Citrus tristeza virus (CTV) universal, CTV genotype T30, CTV genotype VT, Citrus psorosis virus, Citrus tatter leaf virus, Citrus leaf blotch virus, Citrus exocortis viroid, Hop stunt viroid, and Citrus leprosis virus in a plant sample, the method comprising: extracting RNA from said sample; performing a multiplex branched DNA signal amplification reaction; wherein the reaction comprises a probe set that targets Citrus tristeza virus (CTV) universal, a probe set that targets CTV genotype T30, a probe set that targets CTV genotype VT, a probe set that targets Citrus psorosis virus, a probe set that targets Citrus tatter leaf virus, a probe set that targets Citrus leaf blotch virus, a probe set that targets Citrus exocortis viroid, a probe set that targets Hop stunt viroid, and a probe set that targets Citrus leprosis virus, wherein each probe set comprises at least one capture extender probe and a at least one label extender probe, and wherein; detecting the presence or absence of a signal above background from at least one of the probe sets, wherein the presence of the signal is indicative of the presence of the pathogen that the probe set targets.