Patent ID: 9365825
Filing Date: 2016-06-14
CPC Classification: C07K,C12N

Claim Text:
1. A method for producing a population of conditionally immortalized adult stem cells comprising: providing one or more adult stem cells with a) an exogenously synthesized Myc-fusion polypeptide that promotes one or more of cell survival or proliferation; and b) an exogenously synthesized Bcl-2 domain polypeptide that inhibits apoptosis; and wherein the Myc-fusion polypeptide is provided to the one or more adult stem cells at intervals of at least 24 hours, and wherein the Myc-fusion polypeptide is continuously provided to the cells for at least 24 hours; wherein the Bcl-2 domain polypeptide is provided to the one or more adult stem cells at intervals of at least 24 hours, so as to produce a population of conditionally immortalized adult stem cells; and wherein the Myc-fusion polypeptide comprises a Myc polypeptide fused to a protein transduction domain.