Patent ID: 9339812
Filing Date: 2016-05-17
CPC Classification: B01L,B29C,B29L,C12M,C12N,C12Q

Claim Text:
1. A method for processing and detecting nucleic acids from a biological sample, with a cartridge including a fluidic pathway coupled to a sample port and a reagent port, wherein the method comprises: aligning the cartridge at a cartridge platform of a molecular diagnostic module, the cartridge platform having a set of slots, and the molecular diagnostic module having a cam module contacting a set of pins aligned with the set of slots; dispensing the biological sample into the fluidic pathway through the sample port of the cartridge; moving the cam module by transitioning the actuator into an extended configuration, thereby displacing a subset of the set of pins through the set of slots of the cartridge platform and occluding the fluidic pathway at a subset of occlusion positions to form a first truncated portion of the fluidic pathway for controlling the flow of the biological sample through the fluidic pathway; separating a volume of nucleic acids from the biological sample within the first truncated portion of the fluidic pathway; removing a portion of the volume of nucleic acids from the fluidic pathway through at least one of the reagent port and the sample port of the cartridge; combining the portion of the volume of nucleic acids with a molecular diagnostic reagent to produce a nucleic acid-reagent mixture; and delivering the nucleic acid-reagent mixture through a second portion of the fluidic pathway to a detection chamber.