Patent ID: 9505822
Filing Date: 2016-11-29
CPC Classification: A61K,A61P,C07K

Claim Text:
1. A method of inhibiting angiogenesis, the method comprising contacting endothelial cells associated with the angiogenesis with a single chain vascular endothelial growth factor (VEGF) variant polypeptide, comprising: a first VEGF polypeptide comprising the amino acid sequence of SEQ ID NO: 1, and having at least one substitution selected from the group consisting of V15A, M18R, R23K, L32S, F36L, E44G, I76T, H86Y, Q87R, and Q89H, and a second VEGF polypeptide comprising the amino acid sequence of SEQ ID NO: 1, and having at least one substitution selected from the group consisting of K16R, D41N, E42K, P53S, D63N, M78V, and R82G, wherein the first VEGF polypeptide and the second VEGF polypeptide are covalently linked by an amino acid linker.