Patent ID: 9340823
Filing Date: 2016-05-17
CPC Classification: C12Q

Claim Text:
1. A method for classifying a kidney tumor sample obtained from a human subject as oncocytoma, clear cell renal cell carcinoma (RCC), papillary RCC, or chromophobe RCC, the method comprising: (a) obtaining a kidney tumor sample from the human subject; (b) determining by real-time PCR (RT-PCR) an expression profile of SEQ ID NOS: 1, 4, 6, 8, 10, 12, 14, 16, 18, 20, 22, 24-26, 28, 30, 32, 34, 36, 38, 41, 45, 47, 48, 56, 58, 60, 62, and 66 in the sample, wherein said RT-PCR comprises contacting the sample with forward and reverse primers, wherein each forward primer comprises at least 15-21 nucleotides identical to one of SEQ ID NOS: 1, 4, 6, 8, 10, 12, 14, 16, 18, 20, 22, 24-26, 28, 30, 32, 34, 36, 38, 41, 45, 47, 48, 56, 58, 60, 62, and 66, and wherein the forward primer comprising at least 15-21 nucleotides identical to SEQ ID NO: 14 comprises the sequence of SEQ ID NO: 84; (c) comparing said expression profile to one or more reference values; and (d) classifying the kidney tumor sample as oncocytoma, clear cell RCC, papillary RCC, or chromophobe RCC based on the expression profile of SEQ ID NOS: 1, 4, 6, 8, 10, 12, 14, 16, 18, 20, 22, 24-26, 28, 30, 32, 34, 36, 38, 41, 45, 47, 48, 56, 58, 60, 62, and 66 in the sample relative to the reference value, wherein: