Patent ID: 9359411
Filing Date: 2016-06-07
CPC Classification: A61K,C07K,C12N

Claim Text:
1. A subunit vaccine for protecting pigs against classical swine fever virus (CSFV) infection, which comprises a recombinant truncated yE2 subunit and a veterinarily acceptable adjuvant, wherein the recombinant truncated yE2 subunit is yE2 subunit E2N330 having the amino acid residues 1-330 of yE2 glycoprotein produced by a method comprising the steps of: (a) cloning a coding region for amino acid residues 1-330 of E2 glycoprotein with the first arginine codon changed to AGA into the yeast expression vector pGAPZαC to construct a recombinant expression plasmid; (b) transforming the recombinant expression plasmid obtained in step (a) into Pichia pastoris host cells to obtain transformant cells; (c) cultivating the transformant cells under a condition for expression and secretion of truncated yE2 glycoprotein into a culture medium; and (d) isolating and purifying the recombinant truncated yE2 glycoprotein from the supernatant of the culture medium of step (c).