Patent ID: 9435786
Filing Date: 2016-09-06
CPC Classification: C12N,C12Q,G01N,G06T

Claim Text:
1. A method comprising the steps of: i) measuring flatness of at least one cultured pluripotent stem cell to determine whether the cultured pluripotent stem cell or a cultured pluripotent stem cell population is differentiated by applying a Student's t-test to compare the flatness of the cultured pluripotent stem cell or the cultured pluripotent stem cell population with those of undifferentiated pluripotent stem cells and differentiated cells, and ii) identifying and removing at least one cultured pluripotent stem cell that is determined to be differentiated, wherein the flatness of the cultured pluripotent stem cell or the cultured pluripotent stem cell population is measured from at least one tomographic image of a three-dimensional image of the cultured pluripotent stem cell or the cultured pluripotent stem cell population, wherein the measured flatness is flatness of a surface of the cultured pluripotent stem cell roughly parallel to a bottom surface of the cultured pluripotent stem cell attached to a culture vessel or flatness of a surface of the cultured pluripotent stem cell population roughly parallel to a bottom surface of the cultured pluripotent stem cell population attached to a culture vessel, and wherein the measured flatness of a differentiated cell or a differentiated cell population is statistically different from the measured flatness of an undifferentiated pluripotent stem cell or an undifferentiated pluripotent stem cell population.