Patent ID: 9399796
Filing Date: 2016-07-26
CPC Classification: C12Q

Claim Text:
1. A pre-amplification reaction mixture for use in simultaneously amplifying at least two different target nucleic acid sequences contained in a sample to generate first amplification products, wherein the pre-amplification reaction mixture comprises (a) an enzyme selected from the group consisting of: a polymerase, an RNA-dependent DNA polymerase, a DNA-dependent DNA polymerase, a DNA-dependent RNA polymerase, a reverse transcriptase, an RNase, or a combination thereof; (b) an immobilized probe attached to a solid support; (c) at least two different target capture oligomers, wherein each of said target capture oligomers comprises (i) a target specific sequence and (ii) an immobilized probe-binding region that binds to the immobilized probe; and (d) at least two different amplification oligomer complexes, wherein each of said amplification oligomer complexes comprises a first amplification oligomer member having a first target specific sequence that is joined to a second amplification oligomer member having a second target specific sequence, wherein the first amplification oligomer member is a non-promoter primer and the second amplification oligomer member is a promoter primer, wherein each of said target capture oligomers and each of said amplification oligomer complexes specifically hybridizes to a different target nucleic acid sequence, and wherein each target nucleic acid sequence is specifically targeted by one of said target capture oligomers and one of said amplification oligomer complexes, and wherein the pre-amplification reaction mixture does not contain any target-specific amplification oligomers that are not joined to form one of the at least two amplification oligomer complexes.