Patent ID: 9493791
Filing Date: 2016-11-15
CPC Classification: C12N,C12P,C12Y,Y02P

Claim Text:
1. Recombinant microbial cells capable of producing of isoprene, wherein the cells comprise one or more heterologous nucleic acids encoding a polypeptide having phosphoketolase activity and (i) one or more nucleic acids encoding one or more polypeptides of the complete MVA pathway and (ii) a heterologous nucleic acid encoding an isoprene synthase polypeptide, wherein culturing of said recombinant cells in a suitable media provides for the production of isoprene, and wherein the production of isoprene occurs with lower acetate accumulation in the media as compared to cells that do not express one or more heterologous nucleic acids encoding a polypeptide having phosphoketolase activity.