Patent ID: 9399798
Filing Date: 2016-07-26
CPC Classification: B01L,C07H,C12Q

Claim Text:
1. A method of identifying a plurality of bases in a target nucleic acid comprising the following steps: (i) attaching the 5′-end of a primer to a solid surface; (ii) hybridizing a target nucleic acid to the primer attached to the solid surface to form a hybridized primer/target nucleic acid complex; (iii) obtaining a polymerase and a compound of formulas I-VII, with the proviso that compounds of different formulas I-VII have different reporters: (iv) reacting the hybridized primer/target nucleic acid complex with a polymerase and one or more of the compounds of step (iii) to form a growing primer strand via a polymerase reaction; (v) imaging the growing primer strand to identify the incorporated compound of step (iv) via its reporter; (vi) exposing the solid surface with the growing primer strand to a light source to remove a photocleavable terminating moiety of the formula: (vii) repeating steps (iv) through (vi) one or more times to identify a plurality of bases in the target nucleic acid, where the extended primer of step (vi) of the previous cycle reacts in place of the hybridized primer/target nucleic acid complex in step (iv) of the subsequent cycle.