Patent ID: 9388454
Filing Date: 2016-07-12
CPC Classification: C12P,C12Q,G01N

Claim Text:
1. A method for producing a protein-nucleic acid conjugate, the method comprising: a 3′-terminal addition step of adding at least one nucleoside derivative to a 3′-terminal of a nucleic acid using a 3′-terminal addition enzyme which adds a nucleotide to a 3′-terminal of a nucleic acid, the nucleoside triphosphate derivative comprising a glutamine (Gln) residue, and a protein binding step of binding a protein to the glutamine (Gln) residue of the nucleoside triphosphate derivative, using a transglutaminase (TGase), the protein comprising a lysine (Lys) residue and a labeling moiety, wherein the nucleoside triphosphate derivative is represented by formula (1) shown below: wherein each of X and Y independently represents an alkylene group having a carbon number of 1 to 48 or an alkenylene group having a carbon number of 2 to 48 which may be substituted with an ethenylene group, —(C wherein Z represents an alkyl group having a carbon number of 1 to 48, an alkoxy group having a carbon number of 1 to 48, an aryl group having a carbon number of 6 to 48, an aryloxy group having a carbon number of 6 to 48, an arylalkyl group having a carbon number of 7 to 48, or an arylalkyloxy group having a carbon number of 7 to 48, which may be substituted with a dinitrophenyl group or an L-3,4-dihydroxyphenyl group, wherein at least one of Y and Z may be independently substituted with an amino acid other than Lys, wherein B represents a hydrogen atom or a hydroxyl group, wherein C represents a hydrogen atom or a hydroxyl group, wherein m represents 0 or 1, and wherein the 3′-terminal addition enzyme introduces the at least one nucleoside triphosphate derivate in a non-template dependent manner.