Patent ID: 9359630
Filing Date: 2016-06-07
CPC Classification: C12N,C12P

Claim Text:
1. A method for producing a polypeptide, comprising: (a) cultivating a fungal host cell in a medium conducive for the production of the polypeptide, wherein the fungal host cell comprises a polynucleotide encoding the polypeptide operably linked to a promoter selected from the group consisting of (i) a promoter comprising SEQ ID NO: 1 or a subsequence thereof that retains promoter activity, (ii) a promoter comprising a nucleotide sequence that hybridizes under at least high stringency conditions with SEQ ID NO: 1 or the full-length complement thereof, wherein high stringency conditions are defined as prehybridization and hybridization at 42° C. in 5×SSPE, 0.3% SDS, 200 micrograms/ml sheared and denatured salmon sperm DNA, and 50% formamide, and washing three times each for 15 minutes using 2×SSC, 0.2% SDS at 65° C.; and (iii) a hybrid or tandem promoter of (i) or (ii); wherein the polynucleotide encoding the polypeptide is foreign to the promoter; and (b) isolating the polypeptide from the cultivation medium.