Patent ID: 9410182
Filing Date: 2016-08-09
CPC Classification: C12Q,G01N

Claim Text:
1. A method of screening a test substance as an inhibitor of sialyltransferase enzyme activity comprising: combining a sugar donor, an acceptor substrate, a sialyltransferase enzyme, a test substance, and a phosphatase in a buffer solution to produce a product, wherein the phosphatase is 5′-nucleotidase, wherein the phosphatase is not alkaline phosphatase, and wherein the product includes an amount of free phosphate; measuring the amount of free phosphate present in the product; using the measured amount of free phosphate to calculate the activity of the sialyltransferase enzyme in the presence of the test substance; comparing the calculated activity of the sialyltransferase enzyme in the presence of the test substance to a known or measured activity of the sialyltransferase enzyme without the test substance; and designating the test substance as an inhibitor of the activity of the sialyltransferase enzyme when the calculated activity of the sialyltransferase enzyme in the presence of the test substance is lesser than the known or measured activity of the sialyltransferase enzyme without the test substance.