Patent ID: 9376716
Filing Date: 2016-06-28
CPC Classification: C12N,C12Q,C40B,G16B

Claim Text:
1. A method for identifying one or more polymorphisms in nucleic acid samples, comprising the steps of: (a) providing a plurality of nucleic acid samples of interest; (b) performing a complexity reduction on each of the samples to provide a plurality of libraries of the nucleic acid samples, (c) ligating sequencing adapters carrying a 3′-T overhang to the complexity reduced nucleic acid samples in the libraries and combining the libraries; (d) sequencing at least a portion of the combined libraries to obtain sequences; (e) aligning each of the sequences obtained in step (d) to obtain an alignment; and (f) determining one or more polymorphisms between the plurality of nucleic acid samples in the alignment of step (e).