Patent ID: 9512468
Filing Date: 2016-12-06
CPC Classification: C12Q

Claim Text:
1. A method for determining the presence of at least one nucleic acid in a sample, comprising: providing magnetic nanoparticles and detectable nanoparticles to the sample, wherein the magnetic nanoparticles and detectable nanoparticles respectively comprise oligonucleotides attached thereto, and the detectable nanoparticles comprise at least one kind of nanoparticles with detectable signals distinct from the others, and the oligonucleotides attached on each kind of the detectable nanoparticles are complementary to a region of one of the nucleic acids in the sample; reacting the magnetic and detectable nanoparticles with the sample to hybridize the oligonucleotides on the magnetic and detectable nanoparticles respectively to the nucleic acid and extend the oligonucleotides on the magnetic and detectable nanoparticles respectively to form complementary sequences of the nucleic acid; and detecting signals from each kind of the detectable nanoparticles to determine the presence of the at least one nucleic acid in the sample, wherein the detectable nanoparticles are SERS-active nanoparticles, and the SERS-active nanoparticles are gold nanoparticles with a diameter of 51 nm ±2 nm.