Patent ID: 9475870
Filing Date: 2016-10-25
CPC Classification: A61K,C07K

Claim Text:
1. A single domain specific binding molecule comprising the structure FW1-CDR1-FW2-HV2-FW3a-HV4-FW3b-CDR3-FW4 in which the Framework Regions FW1, FW2, FW3a, FW3b, and FW4, the Complementarity Determining Regions CDR1 and CDR3, and the Hypervariable Regions HV2, and HV4 comprise amino acid sequences in which FW1 comprises the sequence TRVDQTPRTATRETGESLTINCVLT (SEQ ID NO: 1) or a humanized derivative of SEQ ID NO: 1 in which at least one amino acid of SEQ ID NO: 1 is replaced by a corresponding amino acid of SEQ ID NO: 65, wherein the humanized derivative is at least 50% identical to SEQ ID NO: 1; FW2 comprises the sequence TYWYRKNPGS (SEQ ID NO: 2); FW3a comprises the sequence GRYVESVN (SEQ ID NO: 3) or a humanized derivative of SEQ ID NO: 3 in which one amino acid of SEQ ID NO: 3 is replaced by a corresponding amino acid of SEQ ID NO: 65, wherein the humanized derivative is at least 85% identical to SEQ ID NO: 3; FW3b comprises the sequence FSLRIKDLTVADSATYICRA (SEQ ID NO: 4) or a humanized derivative of SEQ ID NO: 4 in which at least one amino acid of SEQ ID NO: 4 is replaced by a corresponding amino acid of SEQ ID NO: 65, wherein the humanized derivative is at least 55% identical to SEQ ID NO: 4; FW4 the sequence GAGTVLTVN (SEQ ID NO: 5) or a humanized derivative of SEQ ID NO: 5 in which at least one amino acid of SEQ ID NO: 5 is replaced by a corresponding amino acid of SEQ ID NO: 65, wherein the humanized derivative is at least 44% identical to SEQ ID NO: 5; CDR1 comprises a sequence at least 60% identical to the sequence DTSYPLYS (SEQ ID NO: 6); CDR3 comprises a sequence selected from the group consisting of MGTNIWTGD (SEQ ID NO: 7), MATNIWTGD (SEQ ID NO: 8), and MSTNIWTGD (SEQ ID NO: 11); HV2 comprises the sequence SNKEQISIS (SEQ ID NO: 26); and HV4 comprises a sequence at least 60% identical to the sequence KGTKS (SEQ ID NO: 27).