Patent ID: 9322065
Filing Date: 2016-04-26
CPC Classification: A61P,C12Q

Claim Text:
1. A method of detecting incidence or recurrence of bladder cancer or a predisposition to the incidence or recurrence of bladder cancer in a sample comprising bladder cells, the method comprising: (a) reacting the sample with a reagent which selectively modifies unmethylated cytosine residues in DNA contained in the sample to produce detectable modified residues but which does not modify methylated cytosine residues; (b) determining methylation status of the promoter region of the TWIST1 gene in the sample comprising the primer binding sites for SEQ ID NO:22 and SEQ ID NO:23 and the probe binding site for SEQ ID NO:24, wherein determining methylation status comprises hybridizing to the promoter region of the TWIST1 gene comprising the primer binding sites for SEQ ID NO:22 and SEQ ID NO:23 and the probe binding site for SEQ ID NO:24 a primer and/or probe that hybridizes to the promoter region of the TWIST1 gene comprising the primer binding sites for SEQ ID NO:22 and SEQ ID NO:23 and the probe binding site for SEQ ID NO:24; and (c) detecting hypermethylation of the promoter region of the TWIST1 gene comprising the primer binding sites for SEQ ID NO:22 and SEQ ID NO:23 and the probe binding site for SEQ ID NO:24 to indicate incidence or recurrence of bladder cancer or a predisposition to the incidence or recurrence of bladder cancer.