Patent ID: 9528097
Filing Date: 2016-12-27
CPC Classification: C12N,C12Y

Claim Text:
1. An improved method of extraction and detection of the enzyme Amy797E from a biological sample, wherein the biological sample comprises maize transgenic event 3272, comprising: a) homogenizing the biological sample in the presence of extraction buffer and a solubility-promoting compound, wherein the solubility-promoting compound is an amylolytic enzyme; b) incubating the sample to permit solubilization of the enzyme Amy797E; and c) centrifuging the sample to separate the soluble and insoluble fractions, wherein the enzyme Amy797E fractionates into the soluble fraction, and d) detecting the enzyme Amy797E in the soluble fraction by immunoassay, wherein the enzyme Amy797E extracted from the biological sample can be detected with a limit of detection of no greater than 1:1000 maize kernels.