Patent ID: 9448242
Filing Date: 2016-09-20
CPC Classification: G01N

Claim Text:
1. A screening method for identifying an antalgic compound without any constipating and/or respiratory depressive effect, the method consisting of the following steps: a) providing a candidate compound; b) determining whether said candidate compound activates a potassium channel selected from TREK-I, TRAAK and TREK-2; c) measuring an antalgic effect of said candidate compound from step (b) that activates a potassium channel selected from TREK-I, TRAAK and TREK-2, in vivo on non-human model animal in terms of whether the candidate compound attenuates or abolishes pain generated during the step; and d) measuring the constipating and/or respiratory depressive effect of said candidate compound from step (b) that activates a potassium channel selected from TREK-I, TRAAK and TREK-2 in vivo on non-human model animal, wherein reduction of defecation is measured for the constipating effect and wherein decrease of respiratory frequency is measured for the respiratory depressive effect.