Patent ID: 9249232
Filing Date: 2016-02-02
CPC Classification: C07K

Claim Text:
1. A method for obtaining at least one humanized antibody with specificity for a selected antigen comprising: (a) providing multiple donor antibody V (b) selecting for each donor region sequence of step (a), an appropriate human V (c) transferring CDR-H1, CDR-H2, CDR-H3, CDR-L1, CDR-L2 and CDR-L3 of each donor antibody region sequence onto each of the selected appropriate human acceptor framework sequences to obtain a panel of humanized antibody sequences, (d) expressing the humanized antibody sequences; (e) screening the expressed humanized antibody sequences for antigen-binding activity; and (f) selecting at least one screened high affinity humanized antibody sequence;