Patent ID: 9336349
Filing Date: 2016-05-10
CPC Classification: C12Q,G16B

Claim Text:
1. A method for producing an RNA and DNA-containing probe for use in a method of detecting a target nucleic acid based on whether or not the probe is cleaved by ribonuclease H after the probe is hybridized to the target nucleic acid which comprises the step of preparing a nucleic acid fragment containing RNA and DNA having the same sequence as a partial nucleotide sequence obtained by the RNA nucleotide conversion which is generated by a the method for processing nucleotide sequence information, wherein the method for processing nucleotide sequence information is carried out by a device for processing the nucleotide sequence information and comprises the steps of: (1) by the device, outputting inputted data of a nucleotide sequence comprising position information of a target nucleotide wherein the inputted data of the nucleotide sequence comprise the target nucleotide, the nucleotide adjacent to the 5′ side of the target nucleotide, and the nucleotide adjacent to the 3′ side of the target nucleotide; (2) by the device, generating data of a nucleotide sequence comprising the position information of the target nucleotide which is complementary to the outputted data of the nucleotide sequence comprising the position information of the target nucleotide; (3) by the device, generating data of a nucleotide sequence which is obtained by converting the target nucleotide or the nucleotide adjacent to the target nucleotide in the nucleotide sequence into an RNA, in the data of the nucleotide sequence comprising the position information of the target nucleotide or the nucleotides each adjacent to the target nucleotide and the data of the complementary nucleotide sequence thereof; and (4) by the device, generating data of a partial nucleotide sequence comprising the position information of the target nucleotide or the nucleotides each adjacent to the target nucleotide, from the data of the nucleotide sequence and the data of the complementary nucleotide sequence thereof obtained by the RNA nucleotide conversion, wherein (a) the number of nucleotides in the partial nucleotide sequence is 7 to 14, (b) the Tm value of the partial nucleotide sequence is 25 to 40° C., wherein the Tm value is calculated under conditions having a DNA concentration of 500nM and a salt concentration of 50 mM, and (c) the target nucleotide or the nucleotide adjacent to the target nucleotide in the nucleotide sequence is located at a position between 3 and 5 nucleotides from the 3′ end of the nucleotide sequence or between 3 and 5 nucleotides from the 5′ end of the nucleotide sequence, (5) by the device, using the data of the partial nucleotide sequence obtained by the RNA nucleotide conversion to generate data of a nucleotide sequence of an RNA and DNA-containing probe used in a method of detecting a target nucleic acid based on whether or not the probe is cleaved by ribonuclease H after the probe is hybridized to target nucleic acid, and wherein the method more efficiently generates the data of the nucleotide sequence of an RNA and DNA-containing probe wherein the probe more efficiently detects the target nucleic acid and detects the target nucleic acid with a high level of specificity.