Patent ID: 9400283
Filing Date: 2016-07-26
CPC Classification: G01N

Claim Text:
1. A method for evaluating influence of ATP Binding Cassette (ABC) transporters on a penetration of a drug in human skin, the method comprising the steps of: (1) preparing ex vivo human skin samples, and maintaining skin samples in organoculture conditions; (2) contacting a first skin sample with a specific ABC transporter inhibitor solubilized in a vehicle in organoculture conditions; (3) contacting a second skin sample (control) with a vehicle in organoculture conditions; (4) contacting both control and ABC transporter inhibitor treated skin samples with a drug in organoculture conditions; (5) determining for each skin sample an amount of drug collected in a liquid receptor (absorption) after penetration through the skin; (6) determining for each skin sample an amount of drug located in the skin samples (distribution); and (7) comparing the level of uptake of the drug in the first skin sample (with inhibitor) with that of the control skin sample (without inhibitor).