Patent ID: 9273354
Filing Date: 2016-03-01
CPC Classification: B01J,B01L,C12Q,C40B,G01N

Claim Text:
1. A method of sequencing multiple target polynucleotides in parallel using a sequencing system, comprising: providing a flow chamber comprising a plurality of ridges within the flow chamber, the flow chamber comprising multiple microparticles immobilized between the ridges, each microparticle of the multiple microparticles including a target polynucleotide to be sequenced immobilized on the microparticle, the average distance between centers of adjacent microparticles being less than two microparticle diameters, and the ridges being of a height less than a diameter of a microparticle and of a length greater than a plurality of said diameters; performing a sequence determination of the target polynucleotides immobilized on the microparticles in the flow cell; and obtaining data that provides information about the plurality of target polynucleotides based on the sequence determination.