Patent ID: 9234247
Filing Date: 2016-01-12
CPC Classification: C12Q

Claim Text:
1. A method for detecting mutation(s) in a nucleotide sequence comprising: performing a nucleic acid amplification reaction in the presence of DNA polymerase having exonuclease activity by using any one of the following oligonucleotides (a) to (d) or a salt thereof as a primer, using a probe labeled with a reporter fluorescent dye and a quencher dye, a dye labeled probe, or an intercalator, and using a nucleic acid in a sample as a template, and detecting a reaction product in real time; (a) an oligonucleotide, or a salt thereof; (b) an oligonucleotide, or a salt thereof; (c) an oligonucleotide, or a salt thereof; (d) an oligonucleotide, or a salt thereof, wherein the nucleotide having a modification that inhibits the reaction of nucleic acid synthesis is represented by formula [I] or [II], wherein B represents a nucleic acid base; R wherein B represents a nucleic acid base; and R wherein no reaction products are obtained if the 3′ end nucleotide of the oligonucleotide is cleaved as a result of the exonuclease activity of the DNA polymerase and the nucleotide having the modification which inhibits a reaction of nucleic acid synthesis at the second position from the 3′ end of the oligonucleotide becomes the 3′ end nucleotide of the oligonucleotide.