Patent ID: 9395370
Filing Date: 2016-07-19
CPC Classification: C07K,C40B,G01N

Claim Text:
1. A method of identifying a pair of monoclonal antibodies for use in a sandwich immunoassay prior to the establishment of individual hybridoma clones, the method comprising in the following order, (a) contacting an array of non-clonal different hybridoma culture supernatants to an array of antibody-binding molecules, wherein the antibody-binding molecules are linked to a solid support, under conditions to allow for binding of antibodies in the supernatant to the antibody-binding molecule, wherein hybridomas in the hybridoma cultures were not previously isolated to form clonal lines; (b) separating unbound components of the supernatants from the support; (c) altering the conditions of solution surrounding the solid support, thereby eluting polyclonal antibodies from the antibody-binding molecules, (d) separating the solid support from the solution comprising the eluted polyclonal antibodies of step (c) to create an array of elutions comprising antibodies from the hybridomas; and (e) determining the ability of a first antibody in the array of elutions to bind to an antigen in the presence of a second antibody that binds the antigen, thereby identifying a pair of monoclonal antibodies for use in a sandwich immunoassay.