Patent ID: 9494585
Filing Date: 2016-11-15
CPC Classification: C07K,C12Q,G01N

Claim Text:
1. A detection system comprising: a) a first coupling product (i) comprising a monomer (A) of a whole or membrane form of a protein selected from the group consisting of: Lingo-1, Lingo-2, Lingo-3 and Lingo-4, and (ii) a first probe which is a fluorescent protein selected from the group consisting of GFP (“Green Fluorescent Protein”), YFP (“Yellow Fluorescent Protein”), Enhanced Yellow Fluorescent Protein (eYFP), eGFP, GFP2, GFP10, RGFP ( b) a second coupling product comprising (i) a monomer (B) of a whole or membrane form of a protein selected from the group consisting of: Lingo-1, Lingo-2, Lingo-3 and Lingo-4, and (ii) a second probe which is a luciferase selected from the group consisting of wherein said first coupling product and said second coupling product form a dimer by interaction between said monomers (A) and (B) and, said dimer undergoes a conformational change upon binding to a candidate molecule, and said conformational change causes a change of the detectable signal emitted by said first and second probes, generated by a transfer of energy from the first probe to the second probe, and wherein said system is in the form of a cell suspension, or a culture of adherent cells, membranes or tissues.