Patent ID: 9274060
Filing Date: 2016-03-01
CPC Classification: G01N,Y10T

Claim Text:
1. A method for transmembrane measurement of the oxygen concentration within a space enclosed by a membrane, comprising the steps of: (a) obtaining a transmembrane photoluminescent oxygen probe comprising at least (i) a transmembrane photoluminescent oxygen probe comprising an oxygen impermeable support layer carrying a spot of an oxygen-sensitive photoluminescent dye and a layer of a pressure-sensitive adhesive on a first major surface, (b) adhering the probe to the exterior surface of the membrane with the first major surface of the probe facing towards the membrane, (c) allowing the oxygen concentration in sensible communication with the spot of oxygen-sensitive photoluminescent dye to equilibrate with the oxygen concentration within the space, and (d) ascertaining oxygen concentration within the enclosed space by: (i) repeatedly exposing the equilibrated probe to excitation radiation over time, (ii) measuring radiation emitted by the excited probe after at least some of the exposures, (iii) measuring passage of time during the repeated excitation exposures and emission measurements, and (iv) converting at least some of the measured emissions to an oxygen concentration based upon a known conversion algorithm.