Patent ID: 9453218
Filing Date: 2016-09-27
CPC Classification: C12N,C40B

Claim Text:
1. A method for producing a DNA sequence for a nucleic acid library comprising a plurality of elements, wherein the DNA sequence comprises at least three sections, wherein the first and the third sections in each element have defined sequences that are identical in each element of the nucleic acid library, wherein a central second section comprises at least two adjoining variable codon triplets, wherein at least two oligonucleotide libraries and at least two single stranded oligonucleotides with the following characteristics are used in this method: OR the method comprising: hybridizing the oligonucleotides of the first oligonucleotide library to the first complementary oligonucleotide to generate a first double-stranded DNA library and hybridizing the oligonucleotides of the second oligonucleotide library to the second complementary oligonucleotide to generate a second double-stranded DNA library; ligating the first double-stranded DNA library to the second double-stranded DNA library, thereby producing a library of double-stranded DNA ligation products, the library of double-stranded DNA ligation products comprising the following subsections: removing the spacer element from the library of double-stranded DNA ligation products, thereby producing a library of double-stranded DNA-product comprising the following subsections: