Patent ID: 9284534
Filing Date: 2016-03-15
CPC Classification: C12N,C12Q,C12Y,G01N

Claim Text:
1. A cholesterol oxidase mutant modified at one or more amino acid positions selected from: (a). a position corresponding to position 159 of SEQ ID NO: 1 by substituting the amino acid residue Met with an amino acid residue selected from the group consisting of Phe, Leu, Val, Cys, Ile, Ala, Gln, Tyr, Lys, and Ser or by substituting the amino acid residue Ile with an amino acid residue selected from the group consisting of Phe, Leu, Val, Cys, Ala, Gln, Tyr, Lys and Ser; (b). a position corresponding to position 228 of SEQ ID NO: 1 by substituting the amino acid residue Val, Met or Ile with an amino acid residue selected from the group consisting of Ala, Thr, Lys, Cys, Ser, Gly, Glu, Tyr, Pro, Asn, Gln, Trp and His; and (c). a position corresponding to position 396 of SEQ ID NO: 1 by substituting the amino acid residue Phe with an amino acid residue selected from the group consisting of Trp, Ser, Thr, Lys, Ala, Asn, His and Asp, wherein the cholesterol oxidase mutant has at least about 95% sequence identity to any one of SEQ ID NOS: 1-5.