Patent ID: 9416416
Filing Date: 2016-08-16
CPC Classification: A61P,C12N,C12Q

Claim Text:
1. A one-step method of maintaining the integrity of a biological sample for nucleic acid testing in solution, comprising providing an aqueous mixture at a pH of about 6 to 7 that contains: a control nucleic acid sequence, one or more chaotropes; one or more detergents; one or more reducing agents; one or more chelators; and one or more buffers, collectively and in an amount sufficient to denature proteins, inactivate nucleases, kill pathogens, and release nucleic acid from an organism or cell contained within the biological sample when contacted with the biological sample in solution, wherein: upon contact with the biological sample in solution, the released nucleic acid and the control nucleic sequence acid are each detectable in solution by nucleic acid testing for at least 7 days when stored at a temperature of greater than 4° C. and the control nucleic acid sequence is distinguishable from the released nucleic acid; and the control nucleic acid sequence and the released nucleic acid in solution are detectable by a single nucleic acid testing.