Patent ID: 9522396
Filing Date: 2016-12-20
CPC Classification: B01L,G01N,G06K

Claim Text:
1. A method of automatic detection of plasmodium within a sample comprising: obtaining, for each of a plurality of fields, at least three digital images of a sample stained with a combination of acridine orange and Hoechst for differential staining of DNA and RNA, wherein the images are obtained under at least two different lighting conditions, including a brightfield lighting condition and a fluorescent lighting condition, wherein at least a first image is obtained under a lighting condition causing fluorescence of DNA in the sample, and at least a second image is obtained under a lighting condition causing fluorescence of RNA in the sample; performing image processing of the images using at least one classification algorithm, said image processing including identifying bodies showing fluorescent staining for DNA in the at least a first digital image and showing fluorescent staining for RNA in the at least a second digital image, extracting one or more visual classification features of the identified bodies, and using at least one machine-learning algorithm to determine a presence or absence of outputting an indication of said determination.