Patent ID: 9499858
Filing Date: 2016-11-22
CPC Classification: C12Q

Claim Text:
1. A kit for detecting an analyte in a sample, comprising: (a) a first proximity member, comprising a first antigen that is conjugated to a single stranded first oligonucleotide comprising, from 3′ to 5′, a first portion and a second portion, wherein the first antigen is capable of forming a complex with an antigen-binding site of an analyte comprising at least two antigen-specific binding sites; and (b) a second proximity member, comprising a second antigen, different from the first antigen, that is conjugated to a single stranded second oligonucleotide comprising, from 5′ to 3′, a first portion and a second portion, where the second antigen is capable of forming a complex with another antigen-binding site of the analyte, and wherein upon complexation of the first antigen and second antigen with the analyte, both the first oligonucleotide and the second oligonucleotide do not directly bind to the analyte and the first portion of the first oligonucleotide hybridizes to the second portion of the second oligonucleotide, leaving the first portion of the second oligonucleotide unbound to either the another antigen-binding site of the analyte or the first oligonucleotide and forming an oligonucleotide bridge from an analyte binding site of the second antigen to the second portion of the second oligonucleotide such that only the 3′ terminus of the second portion of the second oligonucleotide is capable of being extended via a polymerase to form a complement of the second portion of the first oligonucleotide.