Patent ID: 9267175
Filing Date: 2016-02-23
CPC Classification: C12Q,G01N

Claim Text:
1. A method of classifying an adult patient with septic shock as high risk or low risk, the method comprising: identifying an adult patient with septic shock; obtaining a sample from the patient; analyzing the sample to determine the level(s) of one or more biomarkers selected from the group consisting of the biomarkers listed in Table 1, wherein the one or more biomarkers comprise all of C-C chemokine ligand 3 (CCL3), heat shock protein 70 kDa 1B (HSPA1B), interleukin-8 (IL8), C-C chemokine ligand 4 (CCL4), and granzyme B (GZMB); determining whether the level(s) of the one or more biomarkers are elevated above a cut-off level, wherein the presence of an elevated level of one or more biomarkers associated with septic shock in adult patients indicates that the patient has an elevated likelihood of being classified as high risk and the absence of an elevated level of one or more biomarkers associated with septic shock in adult patients indicates that the patient has a reduced likelihood of being classified as high risk, wherein a classification of high risk comprises: a) an elevated level of CCL3 and a highly elevated level of IL8, or b) an elevated level of CCL3, a non-highly elevated level of IL8, and a highly elevated level of GZMB, or c) non-elevated levels of CCL3, HSPA1B, and CCL4, and an elevated level of lactate, or d) a non-elevated level of CCL3, elevated levels of HSPA1B and IL8, and a positive patient history of chronic disease, or e) non-elevated levels of CCL3, IL8, and GZMB, an elevated level of HSPA1B, and a positive patient history of chronic disease, or f) a non-elevated level of CCL3, an elevated level of HSPA1B, a highly elevated level of lactate, a negative patient history of chronic disease, and a patient age of older than 36years, and wherein a classification of low risk comprises: g) non-elevated levels of CCL3, HSPA1B, and lactate, or h) non-elevated levels of CCL3and HSPA1B, and elevated levels of lactate and CCL4, or i) non-elevated levels of CCL3 and IL8, elevated levels of HSPA1B and GZMB, and a positive patient history of chronic disease, or j) a non-elevated level of CCL3, an elevated level of HSPA1B, a negative patient history of chronic disease, and a patient age of 36 years or younger, or k) a non-elevated level of CCL3, a non-highly elevated level of lactate, an elevated level of HSPA1B, a negative patient history of chronic disease, and a patient age of over 36 years, or l) an elevated level of CCL3 and non-highly elevated levels of IL8 and GZMB.