Patent ID: 9259396
Filing Date: 2016-02-16
CPC Classification: A61K,A61P,C07D,Y02P

Claim Text:
1. A method of preparing elongated soy isoflavone nanoparticles having a length/width ratio of 8.3 to 10.8 for improving in vivo bioavailability based on precipitation with compressed antisolvent, the method comprising: providing a supercritical fluid as the compressed antisolvent to a precipitation chamber where said precipitation of the elongated soy isoflavone nanoparticles takes place, wherein said supercritical fluid is supercritical or near supercritical CO dissolving raw soy isoflavone in to an organic solvent to form a solution of soy isoflavone at a concentration of 0.4 mg/mL to 4 mg/mL, wherein said organic solvent is acetone; presetting said precipitation chamber to reach conditions comprising a temperature ranging of 31° C. to 60° C., and a pressure ranging of 85 to 120 bar; introducing said solution of soy isoflavone into the precipitation chamber through a co-axial nozzle after the conditions are reached in said precipitation chamber; mixing said supercritical fluid and said solution of soy isoflavone in said precipitation chamber to precipitate said elongated soy isoflavone nanoparticles with size reduction from the dissolved raw soy isoflavone in the organic solvent under the conditions in said precipitation chamber, wherein a feed rate ratio of said supercritical fluid to said solution of soy isoflavone ranges from 30 to 90 g/mL; providing excess supercritical fluid into the precipitation chamber to remove remaining organic residue from said elongated soy isoflavone nanoparticles; and collecting said elongated soy isoflavone nanoparticles from a metal filter at the bottom of said precipitation chamber after gradual depressurization to atmospheric pressure in said precipitation chamber.