Patent ID: 9340836
Filing Date: 2016-05-17
CPC Classification: C12Q

Claim Text:
1. A method of identifying and differentiating MRSA strains in a sample, comprising: receiving the sample; adding one or more strain specific primer sets, wherein each primer set comprises a first and a second isolated oligonucleotide, each comprising a sequence selected from the group consisting of SEQ ID NO. 9, SEQ ID NO. 10, SEQ ID NO. 13, SEQ ID NO. 14, SEQ ID NO. 17, SEQ ID NO. 18, SEQ ID NO. 21, and SEQ ID NO. 22 to a reaction mixture comprising the sample, wherein the first isolated oligonucleotide is different from the second isolated oligonucleotide; subjecting the mixture to conditions that allow nucleic acid amplification to produce amplification products; and identifying and differentiating MRSA isolates by obtaining an allelic identification of amplification products that signifies the sample as containing MRSA USA100, USA300, USA600, non-MRSA USA100, USA300, or USA600 strains.