Patent ID: 9238842
Filing Date: 2016-01-19
CPC Classification: C12Q

Claim Text:
1. A quick method for detecting the genus Bacillus in samples from oil reservoirs, which comprises: (a) collecting a sample from an oil reservoir; (b) inactivating microbes through high temperature; (c) collecting spores from the inactivated sample; (d) incubating the collected spores in an appropriate medium, and stimulating to resurrect the (e) detecting the resurrected wherein said step of inactivating microbes through high temperature comprises transferring 500 ml of the sample into a sterile flask in a sterile condition, sealing the flask with a sterile ventilate membrane, and placing the flask on an electric heater and boiling the sample for 10 minutes, then cooling the sample at room temperature, wherein said step of collecting spores from the inactivated sample comprises, after the cooling step, transferring the inactivated sample into 50 ml sterile centrifuge tubes in a sterile condition and centrifuging at 10,000×g for 15 minutes to produce a supernatant and a pellet, transferring the supernatant to a sterile container for medium preparation, resuspending the pellet with 1 ml sterile water, and combining the suspension from all the centrifuge tubes into a 10 ml mixture, the mixture containing spores, wherein said medium is compatible with the environment from which the sample was collected, and can provide nutrients for stimulating spores, the medium prepared by solving 2 g molasses, 0.16 g (NH