Patent ID: 9353415
Filing Date: 2016-05-31
CPC Classification: C12P,C12Q,G16B

Claim Text:
1. A method for categorizing a breast cancer tissue sample, said method comprising: a) providing an article of manufacture, said article comprising oligonucleotides for measuring gene expression of genes in the following descriptor groups: (1) PSMD3, TCAP, ERBB2, THRAP4, GRB7, CRK7, GSDML, LENEP, PPARBP; (2) DNALI1, XBP1, AR, FOXA1; (3) GATA3, TFF3, ACADSB, ESR1, CAl2; (4) KRT17, SERPINB5, KRT5, GABRP, ANXA8, TRIM29, S100A2; (5) KRT18, KRT8, PPP2CA, KRT8L2; (6) COL11A1, WNT2, MMP14, PLAU, PLAUR; (7) FN1, THY1, BAPX1, COL5A2; (8) PSMB9, STAT1, PDCD1LG2, GBP1, CXCL10, UBE2L6, TAP1, LAP3; and (9) CEACAM3, CEACAM5, CEACAM6, CEACAM7, said oligonucleotides comprising primer pairs suitable for reverse-transcriptase polymerase chain reaction (RT-PCR) of the genes in the descriptor groups, wherein the primer pairs comprise primers having the sequences of SEQ ID NOs:1-100; b) measuring expression of genes of the descriptor groups in the tissue sample using the primer pairs to carry out an RT-PCR amplification of the genes; c) assigning a positive or negative status for each of the descriptor groups based on whether a majority of genes in a group are overexpressed in the sample; and d) categorizing the breast cancer tissue sample based on a combination of the positive and/or negative statuses so determined, wherein SEQ ID NOs:1-100 are: