Patent ID: 9315863
Filing Date: 2016-04-19
CPC Classification: C12Q

Claim Text:
1. A method for generating a nucleic acid signal extension product during a loop-mediated isothermal amplification reaction, comprising: hybridizing portions of a first nucleic acid signal primer, a first nucleic acid amplification primer, and a first nucleic acid displacement primer to complementary portions of a nucleic acid target sequence, wherein the first nucleic acid signal primer hybridizes to the target sequence downstream of the first nucleic acid amplification primer; wherein the first nucleic acid amplification primer comprises i) a 3′ terminal portion complementary to a portion of the nucleic acid target sequence, and ii) a 5′ terminal portion that does not hybridize but is complementary to a portion of the target sequence; and wherein the first nucleic acid displacement primer hybridizes to the target sequence upstream of the first nucleic acid amplification primer; extending the hybridized first nucleic acid signal primer on the nucleic acid target sequence to produce a first signal extension product; extending the hybridized first nucleic acid amplification primer on the nucleic acid target sequence wherein such extension displaces the first signal extension product from the nucleic acid target sequence and produces a first amplification extension product; extending the hybridized first nucleic acid displacement primer, wherein such extension displaces the first amplification extension product from the nucleic acid target sequence and produces a first displacement extension product; and detecting the first signal extension product.