Patent ID: 9341620
Filing Date: 2016-05-17
CPC Classification: B01L,C12Q,C12Y,G01N

Claim Text:
1. A method of conducting an assay on a sample, the method comprising the steps of: introducing a fluid sample into a microfluidic cartridge such that any analyte present in the sample is capable of being bound by an analyte binding agent and a label present in the cartridge, the cartridge comprising: a sample port for introducing said sample into the cartridge, a substrate comprising one or more microfluidic channels disposed therein, a binding agent within said channel(s) for binding any of said analyte within the sample, and a label for use in detecting an amount of the analyte present in the sample; and a detection area within or wholly comprising an area in which analyte, analyte binding agent, and label binding occurs to produce analyte binding agent/analyte/label complexes , detecting a total level of the label that is complexed or uncomplexed to the analyte present in the detection area in order to obtain a first reference/control value; removing any analyte binding agent/analyte/label complexes from the detection area; detecting a level of any uncomplexed label and any unreacted label that remains in the detection area after removal of said analyte binding agent/analyte/label complexes; and determining said analyte concentration by subtracting the level of uncomplexed/unreacted label from the first reference/control value.