Patent ID: 9243290
Filing Date: 2016-01-26
CPC Classification: C12Q

Claim Text:
1. A method for obtaining the sequence of a polynucleotide, the method comprising: providing a polynucleotide library comprising a plurality of polynucleotide fragments immobilized to a substrate, wherein providing the polynucleotide library comprises: hybridizing a first probe having known nucleic acid sequences to at least a portion of the immobilized polynucleotide library fragments; identifying at least a portion of the first probe hybridized to the immobilized polynucleotide library fragments; assigning to each hybridized fragment a nucleic acid sequence that is the complement of a plurality of nucleotides of the nucleic acid sequence of the first probe hybridized to the immobilized polynucleotide library fragment, wherein the assigning does not require a ligation step; hybridizing a second probe having known nucleic acid sequences to at least a portion of the immobilized polynucleotide library fragments; identifying at least a portion of the second probe hybridized to the immobilized polynucleotide library fragments; and assigning to each hybridized fragment a nucleic acid sequence that is the complement of a plurality of nucleotides of the nucleic acid sequence of the second probe hybridized to the immobilized polynucleotide library fragment, wherein the assigning does not require a ligation step.