Patent ID: 9371382
Filing Date: 2016-06-21
CPC Classification: C07K,C12N,G01N,Y10S

Claim Text:
1. A process for determining concentration of N-terminal pro-brain natriuretic peptide (NT-proBNP) in a bodily fluid sample, comprising: (a) providing isolated polyclonal antibodies specific for the sequence of amino acid residues 26-51 of SEQ ID No: 1, wherein the isolated polyclonal antibodies are immobilized on a solid support; (b) providing a bodily fluid sample from a patient, wherein said bodily fluid sample is suspected of containing antigenic fragments of NT-proBNP; (c) contacting said bodily fluid sample to said isolated polyclonal antibodies immobilized on the solid support to form a first mixture wherein said antigenic fragments of NT-proBNP in said bodily fluid sample are captured by said isolated polyclonal antibodies; (d) washing the first mixture, wherein the first mixture comprises said antigenic fragments of NT-proBNP captured by said isolated polyclonal antibodies immobilized on the solid phase, thereby forming a washed first mixture; (e) providing monoclonal antibodies produced by a hybridoma cell line designated as 6G11-F11-D12 and deposited with the American Type Culture Collection (ATCC) as Accession Number PTA-4844, wherein said monoclonal antibodies are specific for the sequence of amino acid residues 1-25 of SEQ ID NO:1; (f) labeling said monoclonal antibodies with a detector, thereby forming labeled monoclonal antibodies; (g) adding the labeled monoclonal antibodies of step (f) to the washed first mixture of step (d) wherein said monoclonal antibodies bind to said antigenic fragments of NT-proBNP captured by the isolated polyclonal antibodies immobilized on the solid phase in step (c), thereby forming a second mixture; (h) washing the second mixture, wherein the second mixture comprises captured antigenic fragments of NT-proBNP bound by the labeled monoclonal antibodies; (i) adding a reporter that reacts with said detector of the labeled monoclonal antibodies to form a reaction product; and (j) measuring said reaction product to determine concentration of NT-proBNP in said sample.