Patent ID: 9238671
Filing Date: 2016-01-19
CPC Classification: C07H,C12N,C12P,C12Q,C40B

Claim Text:
1. A method for adding a tag to the double-stranded product of a tagmentation reaction, comprising the steps of: (a) providing a double-stranded target nucleic acid and a transposome having a transposase with two transposon end sequences: a “transferred strand” and a “non-transferred strand”; (b) allowing the transposome to fragment the target nucleic acid, whereby the transferred strand is covalently transferred to a first strand of a fragment, and the non-transferred strand remains hybridized to the transferred strand; (c) removing the non-transferred strand from the transferred strand; (d) providing a replacement oligonucleotide that comprises a tag sequence, to hybridize to the transferred strand; and (e) ligating the replacement oligonucleotide to the second strand of the fragment; thereby generating a tagmentation product having a transferred strand and a replacement oligonucleotide.