Patent ID: 9315559
Filing Date: 2016-04-19
CPC Classification: A61K,A61P,C07K,C12N,C12Y

Claim Text:
1. A method for activating an antigen-presenting cell, which comprises: transfecting or transducing an antigen-presenting cell with a nucleic acid comprising a promoter operably linked to a polynucleotide that encodes a chimeric protein, wherein the chimeric protein comprises (i) a membrane targeting region, (ii) a multimeric ligand-binding region, (iii) a cytoplasmic CD40 polypeptide region lacking the extracellular domain, and (iv) a truncated MyD88 polypeptide lacking the TIR domain comprising the amino acid sequence SEQ ID NO: 6, wherein the chimeric protein is expressed in the antigen-presenting cell; and contacting the antigen-presenting cell with a non-protein multimeric ligand that binds to the ligand-binding region; whereby the antigen-presenting cell is activated.