Patent ID: 9249449
Filing Date: 2016-02-02
CPC Classification: C07K,C12N,C12Q

Claim Text:
1. A probe reagent comprising, in order from the N-terminus to the C-terminus, the amino acid sequences of: (1) a fluorescent protein I; (2) a peptide capable of terminating protein degradation,which is a degradation-terminating peptide derived from p105, wherein the degradation-terminating peptide consists essentially of amino acid residues 1-29, 7-29, or 17-29 of SEQ ID NO: 1; (3) a spacer peptide, which consists essentially of amino acid residues 30-34, 30-45, 30-59, or 30-65 of SEQ ID NO: 1 or the amino acid sequence of SEQ ID NO: 15; (4) a fluorescent protein II; and (5) a protein to be degraded, wherein the protein to be degraded is a protein degraded by the ubiquitin-proteasome system, and the probe reagent is degraded from the C-terminus but the degradation of the probe reagent is terminated at the degradation-terminating peptide.