Patent ID: 9476082
Filing Date: 2016-10-25
CPC Classification: C12N,C12P,C12Y,Y02P

Claim Text:
1. A method of producing an isoprenoid compound in a yeast cell, comprising cultivating the yeast cell in a suitable medium, wherein the yeast cell comprises: a) one or more heterologous nucleic acids encoding MEV-1 of SEQ ID. NO: 36, MEV-6 of SEQ ID. NO: 41, MEV-15 of SEQ ID. NO: 50, MEV-18 of SEQ ID. NO: 53, MEV-21 of SEQ ID. NO: 56, or MEV-23 of SEQ ID. NO: 58; b) a heterologous promoter substituted for the endogenous promoter of the cellular aconitase (ACO1) gene; and c) a heterologous nucleotide sequence encoding an ATP-citrate lyase enzyme; wherein the yeast cell has reduced inherent aconitase (ACO1) expression relative to an unaltered yeast cell.