Patent ID: 9285297
Filing Date: 2016-03-15
CPC Classification: B01J,B01L,C12Q,F15C,F16K,G01N,Y10T

Claim Text:
1. A method comprising: diluting a sample into sample portions wherein at least one sample portion contains a single fragment of target analyte; forming in a conduit a plurality of aqueous sample slugs spaced apart from one another by slugs of spacing fluid, and at least one of the aqueous sample slugs comprising at least one target analyte, wherein the forming includes: amplifying a target analyte located in at least one aqueous sample slug; dispensing the aqueous sample slugs one-at-a-time from the conduit onto a substrate to form a pattern of spaced apart aqueous samples on the substrate, the substrate comprising an electrically conductive surface and an immersion fluid configured to have the dispensed aqueous sample slugs immersed therein, and the dispensing onto the substrate comprising dispensing the aqueous sample slugs directly onto the electrically conductive surface of said substrate; reacting the at least one target analyte with a reagent mixture to form at least one target analyte product; imaging the electrically conductive surface to differentiate a target analyte product from non-target analyte products; positioning a collection device comprising a capillary of a capillary electrophoretic analyzer, adjacent the at least one target analyte product on the substrate; injecting at least a portion of the at least one target analyte product into the collection device, wherein the injecting comprises applying a potential to the electrically conductive surface and the capillary; and performing nucleic acid sequencing on the at least one target analyte product with the capillary electrophoretic analyzer.