Patent ID: 9422361
Filing Date: 2016-08-23
CPC Classification: C07K

Claim Text:
1. A process for sequential production of (a) a first concentrate consisting essentially of fibronection (Fn) and (b) a second concentrate consisting essentially of von Willebrand Factor (vWF) comprising: (i) purifying a human plasma cryoprecipitate containing FVIII, Fn and vWF on an anion exchange chromatographic resin to obtain a plasma fraction enriched in Fn and vWF and poor in FVIII, said plasma fraction enriched in Fn and vWF being a waste fraction of a process for isolating Factor VIII (FVIII) from plasma; (ii) sequentially separating Fn and vWF by subjecting said plasma fraction enriched in vWF and Fn to only one subsequent chromatographic purification, wherein the one subsequent chromatographic purification is on strong anionic exchange resin, (a) eluting a first concentrate consisting essentially of Fn from said strong anionic exchange resin with a first elution buffer having an ionic strength suitable for obtaining said first concentrate consisting essentially of Fn, and (b) then eluting a second concentrate consisting essentially of vWF from said strong anionic exchange resin using a second elution buffer having an ionic strength stronger than said first elution buffer for obtaining said second concentrate consisting essentially of vWF.