Patent ID: 9328372
Filing Date: 2016-05-03
CPC Classification: C12N,C12Q,C12Y,G01N

Claim Text:
1. A method for producing a biosensor for measuring glucose in a sample liquid comprising: (i) obtaining a flavin adenine dinucleotide (FAD)-linked glucose dehydrogenase (GLD) produced by cultivating a host cell transformed with a nucleic acid encoding the GLD, and (ii) forming a biosensor comprising an electrode system and an enzyme reaction layer on an electrode of the electrode system, the enzyme reaction layer comprising the GLD and an electron acceptor, wherein: (a) the GLD has an activity toward glucose of catalyzing dehydrogenation of glucose in the presence of the electron acceptor and has an activity toward maltose of 5% or less with respect to the activity toward glucose, and (b) the GLD has an amino acid sequence comprising the sequences set forth in SEQ ID NOs. 8 to 12.