Patent ID: 9394557
Filing Date: 2016-07-19
CPC Classification: C12P,C12Q

Claim Text:
1. A method of linearly amplifying a multiplicity of different short nucleic acid sequences, wherein the multiplicity of short nucleic acid sequences are from the 5′ ends of a multiplicity of target nucleic acids, said method comprising; forming a reaction mixture comprising a multiplicity of different target nucleic acids, a collection of target-specific primers, and an enzyme that catalyzes target-specific primer extension, wherein the target-specific primers can hybridize to the target nucleic acids about 15-35 nucleotides away from the 5′ end of the target nucleic acids; annealing the collection of target-specific primers to the multiplicity of target nucleic acids; extending the multiplicity of target-specific primers with the enzyme that catalyzes target-specific primer extension; and cycling the reaction mixture for at least 20 cycles between an annealing temperature segment of 10° C.-30° C., an extension segment, and a denaturation temperature segment of 35° C.-60° C.; thereby linearly amplifying the multiplicity of different short nucleic acid sequences.