Patent ID: 9284546
Filing Date: 2016-03-15
CPC Classification: A61K,A61P,C12N,C12Y

Claim Text:
1. A method to produce a recombinant human cystathionine β-synthase comprising: a) transfecting a recombinant host cell with a recombinant nucleic acid molecule comprising a first nucleic acid sequence encoding a human cystathionine β-synthase or homologue thereof having human cystathionine β-synthase biological activity, wherein the recombinant nucleic acid molecule comprises a recombinant expression vector operatively linked to the first nucleic acid sequence, the expression vector comprising: b) culturing the transfected host cell from (a) under conditions effective to produce the recombinant human cystathionine β-synthase fusion protein; c) contacting the recombinant human cystathionine β-synthase fusion protein with a human rhinovirus 3C protease to cleave the fusion segment from the recombinant human cystathionine β-synthase; and d) recovering the recombinant human cystathionine β-synthase as a substantially purified recombinant protein wherein the nucleic acid sequence encoding a human cystathionine β-synthase encodes a human cystathionine β-synthase protein having an amino acid sequence having an amino terminal deletion or a carboxyl terminal deletion or both an amino terminal and carboxyl terminal deletion and spanning from a starting position of one of amino acid residues from about 1-83 of SEQ ID NO: 2 at the amino terminus to an ending position of one of amino acid residues 382-550 SEQ ID NO: 2 at the carboxyl terminus.