Patent ID: 9346873
Filing Date: 2016-05-24
CPC Classification: A01K,C07K,C12N

Claim Text:
1. A non-human mammalian cell whose genome comprises an immunoglobulin (Ig) heavy chain locus comprising a transgene comprising a portion of a syngeneic Ig heavy chain locus, said portion comprising all or part of constant region genes encoding a chimeric Ig heavy chain, the transgene comprising a DNA fragment comprising in operable linkage from 5′ to 3′ a DNA sequence of a human variable heavy (VH) segment gene operably linked to a constant region gene comprising a human CH1, human C upper hinge , human C middle hinge , syngeneic mammalian non-human CH2 and syngeneic mammalian non-human CH3 exons, wherein the human CH1 exon is selected from the group consisting of a human mu constant region (Cμ), a human delta constant region (Cδ), a human gamma-1 constant region (Cγ1), a human gamma-2 constant region (Cγ2) and a human gamma-4 constant region (Cγ4), wherein the transgene comprising said human VH segment gene and said syngeneic Ig heavy chain locus replaces all or a portion of the endogenous Ig heavy chain locus, thereby said non-human mammalian cell comprises a genome encoding the chimeric Ig heavy chain.