Patent ID: 9435721
Filing Date: 2016-09-06
CPC Classification: B01L,C12N,G01N

Claim Text:
1. A method for purifying a fluid sample comprising extracellular calcium and cells of a mammalian subject, the cells comprising viable normal cells, the method comprising: applying an initial pulse of fluid shear stress to the sample in vitro, wherein the initial pulse of fluid shear stress is sufficient to induce a fluid shear stress resistance in viable cancerous cells present in the sample such that the magnitude of shear stress required to kill the viable cancerous cells is greater than the magnitude of shear stress required to kill the viable normal cells; and subsequently applying one or more additional pulses of fluid shear stress to the sample in vitro, said fluid shear stress sufficient to selectively kill the viable normal cells relative to the viable cancerous cells such that the ratio of the viable cancerous cells to the viable normal cells is increased.