Patent ID: 9234173
Filing Date: 2016-01-12
CPC Classification: A61K,C12N

Claim Text:
1. A method of making a composition comprising: obtaining an initiating cell population (ICP) of at least ten thousand cells that have a density of less than 1.072 g/ml and at least 25% of which are CD31Bright; and in vitro stimulating the ICP by culturing the ICP in the presence of one or more factors selected from the group consisting of: autologous serum, vascular endothelial growth factor (VEGF), heparin, insulin-like growth factor (IGF), basic fibroblast growth factor (b-FGF), epidermal growth factor (EGF), and ascorbic acid, to differentiate into a progenitor/precursor cell population (PCP) comprising a subpopulation of cells that stain as CD31Bright, demonstrate uptake of Ac-LDL+ and secrete interleukin-8 (IL-8) and angiogenin, wherein the sub-population of cells comprises at least 10% of cells in the PCP, to provide for said composition.