Patent ID: 9284587
Filing Date: 2016-03-15
CPC Classification: C07K,C12N,C12P,C12Y

Claim Text:
1. A method of treating a biomass comprising cellulose, the method comprising contacting the biomass with a host cell that expresses a recombinant chimeric polypeptide comprising at least two segments from at least two different parental cellobiohydrolase I (CBH I) polypeptides, wherein the chimeric polypeptide comprises from N- to C- terminus:(segment 1)-(segment 2)-(segment 3)-(segment 4)-(segment 5)-(segment 6)-(segment 7)-(segment 8); wherein: wherein x wherein the chimeric polypeptide is about 437 or 441 amino acid residues in length, and wherein the chimeric polypeptide has cellobiohydrolase activity and improved thermostability, pH stability and/or expression compared to a CBH I polypeptide comprising SEQ ID NO:2, 4, 6, 8 or 10.