Patent ID: 9375467
Filing Date: 2016-06-28
CPC Classification: A61K,A61P,C12N

Claim Text:
1. A composition for providing immunological protection from an enteric septicemia caused by Edwardsiella ictaluri which comprises a live attenuated strain of Edwardsiella ictaluri , wherein said live attenuated strain of Edwardsiella ictaluri is selected by introducing a gene disrupting mutation in a gene coding for a protein selected from the list consisting of glycine cleavage system (gcvP), serine hydroxymethyltransferase, succinate dehydrogenase, malate dehydrogenase, 2-oxoglutarate dehydrogenase, negative regulator of sigma E activity (rseB), hypothetical protein pEI1_p1, electron transport complex protein RnfB, Fimbrial chaperon protein, Putative RNA one modulator protein pEI1_p4, UDP-glucose 6-dehydrogenase, fumarate reductase (frdA), genes encoding enzymes in the tricarboxylic acid (TCA) cycle, and combinations thereof into an Edwardsiella ictaluri strain, wherein said strain of Edwardsiella ictaluri with said introduced gene disrupting mutation has been screened for virulence by challenging one or more live fish with said strain of Edwardsiella ictaluri with said introduced gene disrupting mutation, wherein a reduction in virulence as compared to a wild type Edwardsiella ictaluri strain identifies said strain of Edwardsiella ictaluri with said introduced gene disrupting mutation as said live attenuated strain of Edwardsiella ictaluri.