Patent ID: 9274121
Filing Date: 2016-03-01
CPC Classification: C07K,C12N,G01N

Claim Text:
1. A method for the qualitative and quantitative detection of a Botulinum toxin, comprising in sequence: (i) providing a composition that includes an artificial construct and an enzyme, wherein the construct has (a) a reporter-containing portion comprising a first fluorophore that is degraded by the enzyme, (b) a cleavage site comprising at least a portion of a motif selected from the group consisting of SNAP-25, synaptobrevin, and syntaxin, wherein the motif interacts with a portion of the Botulinum toxin in a manner that produces a cleavage of the first fluorophore from a remainder of the construct, and (c) a second portion comprising a second fluorophore, wherein the cleavage site is interposed between the reporter containing portion and the second portion; (ii) obtaining a baseline signal emission measurement from the construct; (iii) exposing the Botulinum toxin composition to the composition; and (iv) obtaining a further emission measurement from the reporter fluorophore, wherein the reporter-containing portion and the second portion are positioned in the construct such that FRET emission from the first fluorophore does not show a decreasing trend relative to increasing concentrations of the Botulinum toxin composition, and wherein a normalization emission measurement is obtained from the second fluorophore.