Patent ID: 9513288
Filing Date: 2016-12-06
CPC Classification: A61K,C12N,C12Q,C12Y,G01N

Claim Text:
1. A method of identifying an isoform-selective regulator of PDE3, said method comprising: (a) obtaining a first isolated polypeptide (PDE3A2), wherein said first isolated polypeptide has an amino-acid sequence that is at least 95% homologous to the amino-acid sequence of SEQ ID NO:2; (b) identifying at least one test compound that binds to said first isolated polypeptide (PDE3A2); (c) assaying the at least one test compound for its ability to interfere with binding of said first isolated polypeptide (PDE3A2) to cAMP, cGMP, or another polypeptide; (d) assaying the at least one test compound for its ability to interfere with binding of a second isolated polypeptide (PDE3A1) to cAMP, cGMP, or another polypeptide; (e) identifying said at least one test compound as an isoform-selective regulator of PDE3 when said ability to interfere with binding of cAMP, cGMP, or another polypeptide to said first isolated polypeptide (PDE3A2) is greater than said ability to interfere with binding of cAMP, cGMP, or another polypeptide to said second isolated polypeptide (PDE3A1).