Patent ID: 9518132
Filing Date: 2016-12-13
CPC Classification: A61K,A61P,C07K,G01N

Claim Text:
1. A polypeptide complex comprising: a first polypeptide comprising from N-terminus to C-terminus: a first antigen-binding domain, a first protein monomer, a first thiol residue-containing peptide linker and a second antigen-binding domain, and a second polypeptide comprising from N-terminus to C-terminus: a third antigen-binding domain, a second protein monomer, a second thiol residue-containing peptide linker and a fourth antigen-binding domain, wherein the first protein monomer forms a dimer with the second protein monomer, and each of the first and the second protein monomers comprises a CH3 domain from an immunoglobulin; wherein the first peptide linker and the second peptide linker form a disulfide bond, and each of the first thiol peptide linker and the second peptide linker independently comprises a sequence selected from the group consisting of: SEQ ID NO: 68, SEQ ID NO: 69, and SEQ ID NO: 70; wherein the first antigen-binding domain and the third antigen-binding domain are capable of binding to a first antigen, and the second antigen-binding domain and the fourth antigen-binding domain are capable of binding to a second antigen, and one of the first and the second antigens is human CD3 and the other is human EpCAM or human CD19, and wherein each of the first, second, third, and fourth antigen-binding domains is independently selected from the group consisting of VH, VL, Fab, and scFv.