Patent ID: 9315774
Filing Date: 2016-04-19
CPC Classification: C12M,C12N

Claim Text:
1. A closed system suitable for the aseptic culture of therapeutic cells comprising: (i) a vessel comprising: wherein said vessel defines an internal volume for containing a requisite volume of medium to support a cell culture, (ii) a lid (iii) a vent in the lid comprising a conduit defining an interior orifice and an exterior orifice distal therefrom in fluid communication with each other which allows balancing of pressure during filling the vessel by allowing gases to exit and during the cell-emptying process allows gases to enter the interior volume, wherein the conduit extends from the exterior of the closed system through the lid and extends into the internal volume of the vessel and terminates therein with the interior orifice, and wherein the interior orifice is arranged not to contact the medium in the vessel for all orientations of the vessel including when the system is inverted, wherein the system is emptied under gravity, or wherein the interior orifice is covered with a liquid impermeable membrane or a liquid non-return valve, and wherein the exterior orifice of the vent conduit is attached to a sterile filter, and (iv) a port or ports arranged to provide aseptic introduction of fluids and cells into the vessel, an exit port located on the lid for fluids to exit the system without exposing the system to the external environment which allows cells grown therein to exit the system under gravity when the system is orientated to put the cells in fluid communication with the exit port; wherein the vent and said exit port are co-located in the lid; and wherein the vent is located centrally on the lid and the exit port is located to the side of the lid, wherein said vent conduit extends further into the interior orifice than said exit port does.