Patent ID: 9267931
Filing Date: 2016-02-23
CPC Classification: G01N,Y10T

Claim Text:
1. A method of differentiating and accurately counting a plurality of cells in a sample of whole blood, the method comprising: (i) introducing a first portion of a sample of whole blood into a flow cell of a hematology analyzer at a first flow rate and directing a laser to the flow cell to generate a plurality of first flow rate optical data using a plurality of detectors for detecting light scattering by cells of the sample of whole blood on a plurality of in-flow optical measurement channels, wherein the laser has a wavelength that ranges from 400 nm to 450 nm; (ii) introducing a second portion of the same sample of whole blood into the flow cell at a second flow rate and directing the laser to the flow cell to generate a plurality of second flow rate optical data using the plurality of detectors, wherein the first flow rate and the second flow rate are different; (iii) differentiating and counting a plurality of white blood cells in the sample of whole blood using the first flow rate optical data and at least one algorithm to differentiate and count different types of white blood cells; and (iv) differentiating and counting a plurality of red blood cells and platelets in the sample of whole blood using the second flow rate optical data.