Patent ID: 9322813
Filing Date: 2016-04-26
CPC Classification: G01N

Claim Text:
1. A method of detecting and collecting one or more sample components within a first sample stream in a chromatography system during a chromatographic run comprising: passing the first sample stream through a chromatography column; actively splitting a portion of the first sample stream exiting the column to form a second sample stream that is in fluid communication with an evaporative particle detector, the first sample stream being in fluid communication with an optical absorbance detector, where the first sample stream does not pass through the evaporative particle detector and the second sample stream does not pass through the optical absorbance detector, and where the optical absorbance detector monitors an absorbance spectrum or a range of wavelengths within a spectrum and generates response values from each of the monitored wavelengths; generating a first signal resulting from the response values associated with each of the wavelengths monitored by the optical absorbance detector during the chromatographic run; generating a second signal resulting from a response from the evaporative particle detector during the chromatographic run; and collecting at least one component from the first stream in a fraction collector during the chromatographic run in response to information generated during the chromatographic run, said information consisting of a change in at least one of said signals; wherein the splitting step is performed with use of a splitter pump or a shuttle valve.