Patent ID: 9487821
Filing Date: 2016-11-08
CPC Classification: B01J,C07B,C07H,C12N,C12Q,C40B,G01N

Claim Text:
1. A library of linear double-stranded nucleic acids, wherein said linear double-stranded nucleic acids consist of at least one inherent universal detection target (UDT) proximate to one end of said double strand, at least one non-inherent production center comprising a bacteriophage RNA promoter proximate to the other end of said double strand, and between the at least one inherent UDT and the at least one non-inherent production center, nucleic acid sequences complementary or identical in part or whole to inherent sequences of a library obtained from a sample, wherein said inherent UDT allows detection of the presence of sequences of interest, said sequences of interest being located between said inherent UDT proximate to one end and said bacteriophage promoter proximate to the other end of said double strand in a linear double-stranded nucleic acid of the library, wherein said at least one inherent UDT is selected from the group consisting of (i) a 3′ poly A segment, (ii) a consensus sequence, and (iii) a combination of a 3′ poly A segment and a consensus sequence, wherein said consensus sequence is selected from the group consisting of a signal sequence for poly A addition, a splicing element, a multicopy repeat, and any combination thereof, and wherein said double-stranded nucleic acids do not comprise a non-inherent homopolymeric sequence.