Patent ID: 9512458
Filing Date: 2016-12-06
CPC Classification: B01L,C12Q,G01N

Claim Text:
1. An electrochemical biosensor, comprising: a lower plate, upon which is constructed a working electrode coated with a reagent layer containing an electron transfer mediator and a redox enzyme, and a reference electrode; a middle plate having a precut pattern defining a sample introduction channel composed of a sample entrance for introducing a sample into said electrochemical biosensor, a sample passage and an air vent ; a lower insulator located between the middle plate and the lower plate; an upper insulator located above the middle plate wherein the upper insulator roofs over the air vent, the upper insulator preventing migration of the sample into the air vent; and an upper plate or a cover that forms a sample cell structure, wherein the sample entrance is tapered and narrows toward the sample passage, wherein at least a portion of the working electrode is exposed in the sample passage for direct contact with a sample introduced into the sample entrance; the lower insulator has a precut pattern having the same structure as the precut pattern of the sample passage aligned therewith, the precut pattern of the lower insulator having a bottom wall extending across the sample passage on the same plane as the lower insulator and directly below the sample passage with a hydrophobic sample collection barrier located directly below the sample entrance and between the sample entrance of the middle plate and the lower plate such that the hydrophobic sample collection barrier does not further extend to be below the sample passage; and the hydrophobic sample collection barrier protrudes from the lower plate toward the sample entrance, the hydrophobic sample collection barrier having a predetermined height of 1 μm to 10 μm and extends across the sample entrance when the lower plate, lower insulator middle plate and upper plate are assembled.