Patent ID: 9482660
Filing Date: 2016-11-01
CPC Classification: A61K,A61P,C12N,G01N

Claim Text:
1. A process of preparing a ready to use preparation of hES cells, derivatives of hES cells, or combinations thereof for human transplantation, wherein the derivatives of hES cells are selected from the group consisting of hematopoietic stem cell progenitors, neuronal stem cell progenitors, mesenchymal stem cell progenitors, insulin producing stem cell progenitors, hepatocytic stem cell progenitors, cardiac stem cell progenitors, epithelial stem cell progenitors, and combinations thereof, comprising: (a) introducing the cells in a cell culture medium consisting of minimal essential medium, a progestin, and a β-human chorionic gonadotropin (βhCG) agonist; (b) expanding the cells obtained in (a) at a temperature of about 34° C. to about 38° C. in an environment of about 3.5% to about 6% carbon dioxide for about 12 hours to about 48 hours; (c) centrifuging the cells obtained in (b) to obtain a pellet, and (d) suspending the pellet in a biocompatible solution.