Patent ID: 9458207
Filing Date: 2016-10-04
CPC Classification: C07K

Claim Text:
1. A method for refolding granulocyte colony stimulating factor (G-CSF) from inclusion bodies, comprising: a) solubilising G-CSF in the presence of a solubilising agent selected from the group consisting of a detergent, a tenside and a surfactant; b) performing an oxidation and first refolding step, comprising incubating the solubilised G-CSF in the presence of an oxidizing agent and the solubilising agent; c) removing the solubilising agent by ion exchange resin adsorption and/or ion exchange chromatography, and optionally performing an acid precipitation; and d) performing a second refolding step at a pH value above pH 7, comprising diluting the G-CSF of step (c) with a low conductivity buffer or water and incubating the diluted G-CSF at a conductivity below 2 mS/cm for more than 12 hours, wherein the solubilizing agent after step c) is below 0.01 mg/ml.