Patent ID: 9506112
Filing Date: 2016-11-29
CPC Classification: C12Q

Claim Text:
1. A method comprising: providing a plate including a plurality of wells; providing a first mastermix that emits a first spectrum in at least a first well of the plate, wherein the first mastermix includes a passive fluorescence dye that is a free, non-specific fluorescence dye not coupled to a sequence-specific probe; and providing a second mastermix that does not emit the first spectrum in at least a second well of the plate, wherein the second mastermix includes an active fluorescence dye that is initially coupled to a sequence-specific probe that includes a quencher that suppresses fluorescence of the active fluorescence dye; wherein: the second mastermix is not provided in a same well as the first mastermix; the active fluorescence dye can be changed to emit the first spectrum by decoupling the active fluorescence dye from the sequence-specific probe; and the first mastermix is devoid of the active fluorescence dye coupled to the sequence-specific probe that includes the quencher and the second mastermix is devoid of the passive fluorescence dye that is not coupled to a sequence specific probe.