Patent ID: 9493836
Filing Date: 2016-11-15
CPC Classification: C12Q

Claim Text:
1. A method for the diagnosis in vitro, of an acute myeloid leukemia or a myelodysplastic disorder selected from the group consisting of refractory anemia with ringed sideroblasts (RARS), refractory cytopenia with multilineage dysplasia (RCMD), and refractory anemia with excess blasts (RAEB), said method comprising the steps of: a) obtaining a bone marrow sample from a human subject, b) extracting nucleic acid from said bone marrow sample, c) detecting the expression level of 6 genes in said nucleic acid, wherein said 6 genes consist of the nucleotide sequences of SEQ ID NOs: 1 to 6, and wherein said detecting is carried out by contacting said nucleic acid with SEQ ID NOs: 25 to 36, d) comparing the expression level of each of said 6 genes with the expression level of said 6 genes in control samples obtained from healthy bone marrow to establish a gene expression level ratio for each gene of said 6 genes, e) determining that the ratio established in step d) for at least 3 genes from said 6 genes is either ≧2 or ≦0.5, and f) diagnosing said human subject as having an acute myeloid leukemia or a myelodysplastic disorder selected from the group consisting of RARS, RCMD, and RAEB.