Patent ID: 9504936
Filing Date: 2016-11-29
CPC Classification: B01D,B01J,G01N

Claim Text:
1. A method for packing a chromatographic column comprising silica particles, the method comprising the steps of a) introducing the silica particles into the chromatographic column, wherein the chromatographic column has a length of 2 to 10 cm and an inner diameter of 25 μm to 5 cm, and the silica particles have a diameter of 100 nm to 900 nm; b) ultrasonicating the chromatographic column; and c) applying pressure to the silica particles, wherein steps a)-c) are performed simultaneously and wherein a packed chromatographic column with an eddy diffusion term of less than 1 μM is formed, wherein the silica particles are uniformly packed and exhibit no cracks or uninterrupted contact between the inner surface of the column and the silica particles, and wherein the packed chromatographic column is suitable for HPLC, UHPLC, or FPLC.