Patent ID: 9347050
Filing Date: 2016-05-24
CPC Classification: A61K,A61P,C12N,C12P,C12Y,Y02P

Claim Text:
1. A method of directing a glycoprotein to the interior of a mammalian cell, the glycoprotein comprising a mannose-1-phospho-6-mannose moiety, wherein the mannose residue that has a phosphate residue bound at the 6 position is linked to a terminal mannose residue at the 1 position of the terminal mannose residue, the method comprising contacting the cell with the glycoprotein after it has undergone: (a) uncapping of the mannose-1-phospho-6-mannose moiety to mannose-6-phosphate on the glycoprotein; and (b) removal of the terminal mannose residue, wherein the glycoprotein that has undergone (a) and not (b) or (b) but not (a) does not substantially bind to a mannose-6-phosphate receptor on the cell, wherein the glycoprotein that has undergone (a) and (b) does substantially bind to a mannose-6-phosphate receptor on the cell, and wherein steps (a) and (b) are catalyzed by a single mannosidase enzyme that is a family 38 glycosyl hydrolase (GH38 family) mannosidase.