Patent ID: 9322051
Filing Date: 2016-04-26
CPC Classification: C12Q,G01N

Claim Text:
1. A method of probing multiple targets in a biological sample comprising: (a) providing a biological sample containing multiple targets; (b) binding a set of probes to the multiple targets wherein two or more of the probes in said set of probes comprises independently: (c) detecting at least one signal from at least one signal generator from the set of probes bound in step (b); (d) contacting the sample comprising the bound probes of step (b) with a chemical agent, electromagnetic radiation, or a combination thereof and simultaneously deactivating the at least one signal generator detected in step (c) by cleavage of a linker bound to said signal generator and activating at least one signal generator from the set of probes previously quenched by a masking agent wherein the linker and masking agent are linker-mask pairs; (e) detecting at least one signal from the activation of the at least one newly activated signal generators in step (d); (f) contacting the sample comprising the bound probes of the prior step with a chemical agent or electromagnetic radiation or a combination of both and simultaneously deactivating the at least one signal generator detected in the prior step by cleavage of a linker bound to said signal generator and activating at least one signal generator previously quenched by a masking agent wherein the linker and masking agent are orthogonal linker-mask pairs; (g) detecting at least one signal from the activation of the at least signal generator in step (f); and (h) optionally repeating steps (f)-(g).