Patent ID: 9494593
Filing Date: 2016-11-15
CPC Classification: G01N

Claim Text:
1. A method for separating nanoparticles with a controlled number of active groups, comprising: providing a plurality of nanoparticles, wherein the surface of said nanoparticles comprises a plurality of first active groups; performing a modification process to modify the surface of said nanoparticles with said first active groups by an amphiphilic oligomer or polymer which has at least one —COOH group to form polymer coated nanoparticles with said first active groups; providing a plurality of functional ligands, wherein the molecular weight of said functional ligands is larger than 3000 g/mol, and said functional ligands comprises at least one second active group and at least one third active group, wherein said functional ligands further comprises a spacer, wherein said spacer comprises polyethylene glycol which has at least one —NH2 group; performing a binding process to bind said polymer coated nanoparticles and said functional ligands together through the connection between said first active groups of said polymer coated nanoparticles and said second active group of said functional ligands by controlling the concentration ratio of the polymer coated nanoparticles to the functional ligands to form polymer coated nanoparticles with a controlled number of functional ligands; and performing a separation process selected from one of the following group: size exclusion chromatography (SEC) and gel electrophoresis to isolate a plurality of polymer coated nanoparticles with a predetermined number of said third active groups from said polymer coated nanoparticles with a controlled number of functional ligand wherein the controlled number ranges from 0 to 10 and the predetermined number is an integer selected from 1 to 10.