Patent ID: 9328369
Filing Date: 2016-05-03
CPC Classification: C12N,C12P

Claim Text:
1. A method for producing a substance, comprising: culturing a bacterium in a culture solution comprising a surfactant mixed therein, wherein said substance is synthesized by said bacterium and said substance is secreted into the culture solution by said bacterium, and maintaining said bacterium in the culture solution at a dried cell density of 1.5 to 500 g/L based on a volume of the culture solution, wherein said surfactant is at least one surfactant selected from the group consisting of polyoxyethylene (3 mol) tridecyl ether sodium acetate, palm oil fatty acid amide propyl dimethyl amino acetate betaine, lauryl dimethylamino acetate betaine, cured palm oil fatty acid amidopropyl dimethyl aminoacetate betaine, sodium cocaminopropionate, sodium polyoxyethylene (3 mol) laurylether acetate, sodium polyoxyethylene (2.5 mol) laurylether sulfate, and sodium N-lauroyl-N′-carboxymethyl-N′-hydroxyethyl ethylenediamine, wherein said substance is a protein, and wherein said bacterium is a gram negative bacterium.