Patent ID: 9459262
Filing Date: 2016-10-04
CPC Classification: G01N,Y10T

Claim Text:
1. A method for determining the percentage of glycated hemoglobin in a sample comprising: contacting hemoglobin released from cells in the sample with a first agent that binds hemoglobin and a second agent that binds only glycated hemoglobin, wherein the first agent is labeled with a first detectable marker and the second agent is labeled with a second detectable marker which is distinguishable from the first detectable marker: immobilizing the detectably labeled hemoglobin on a solid support; measuring signals from the first detectable marker with which the first agent is labeled and the second detectable marker with which the second agent is labeled, immobilized on the solid support; and determining the percentage of glycated hemoglobin in the sample based on the measured signals generated from the first and second detectable markers bound to the glycated hemoglobin; wherein the first agent is an antibody or antigen-binding fragment thereof and the second agent is a boronic acid, or a derivative thereof.