Patent ID: 9429501
Filing Date: 2016-08-30
CPC Classification: C08G,G01N

Claim Text:
1. A method for separating or detecting a tumor cell and/or a cancer cell in a sample containing blood components, the method comprising treating the sample by mixing the sample with a liquid containing a surfactant A and a liquid containing a surfactant B, or with a liquid containing the surfactant A and the surfactant B, wherein the surfactant A is a nonionic surfactant of formula (I) wherein: R EO is an oxyethylene group, n is an average addition mole number of EO in the range of 23 to 50, and R the surfactant B is a nonionic surfactant selected from the group consisting of a sorbitan fatty acid ester, a compound of formula (III), a compound of formula (IV), a compound of formula (VI) and combinations thereof, wherein: R R AO is an oxyalkylene group having a carbon number in the range of 3 to 6, EO is an oxyethylene group, m and n are average addition mole numbers of AO and EO respectively, where m is a number in the range of 1 to 100 and n is a number in the range of 0 to 50, and “/” indicates that the AO group and the EO group may be added at random or as a block regardless of the order; wherein: R EO is an oxyethylene group, n is an average addition mole number of EO in the range of 8 to 22, and R wherein: R EO is an oxyethylene group, n is an average addition mole number of EO in the range of 6 to 160, and R where the surfactant B has a lytic property with respect to an erythrocyte higher than the lytic property of the surfactant A with respect to an erythrocyte, and wherein the method eliminates erythrocytes and leukocytes present in the sample while suppressing damage to the tumor cell and/or the cancer cell.