Patent ID: 9238814
Filing Date: 2016-01-19
CPC Classification: C12N,C12Q

Claim Text:
1. A method for the cleavage of a target RNA comprising: reacting a member selected from the group consisting of a hammerhead ribozyme represented by the formula of SEQ ID NO. 1: a hammerhead ribozyme represented by the formula of SEQ ID NO. 3: and a hammerhead ribozyme represented by the formula of SEQ ID NO. 5: with a member selected from the group consisting of a target RNA represented by formula: 5′-GGAUCGGUAUGUAGCAAUACGU-A-AUCCUAUUGAGCAUGCC-3′ (SEQ ID NO:2), a target RNA represented by formula: 5′-UUCUAGCCUU-C-AGGAGUCAGGGC-3′ (SEQ ID NO:4) and a target RNA represented by formula: 5′-GCAGACAUAUAUGAU-A-CAAUUUGAUCAG-3′ (SEQ ID NO:6), respectively, to give a hammerhead ribozyme-target RNA construct represented by the formula of SEQ ID NOs. 1 and 2[IIIb]: a hammerhead ribozyme-target RNA construct represented by the formula of SEQ ID NOs. 3 and 4[IIIc]:  and a hammerhead ribozyme-target RNA construct represented by the formula of SEQ ID NOs. 5 and 6 [IIId]: wherein arrowhead symbol means a cleaving site.