Patent ID: 9410187
Filing Date: 2016-08-09
CPC Classification: C12Q

Claim Text:
1. A method for detecting the production of a primer extension product in a primer extension reaction mixture, said method comprising: (a) producing a primer extension mixture comprising a template nucleic acid, a nucleic acid polymerase having 3′→5′ exonuclease activity, and a FET labeled oligonucleotide comprising a target-binding sequence, a fluorophore, a nucleic acid intercalator, and a quencher, wherein said nucleic acid intercalator and said quencher are covalently bonded to the 3′ end of said FET labeled oligonucleotide; (b) subjecting said primer extension mixture to primer extension reaction conditions; and (c) detecting a fluorescent signal from said FET labeled oligonucleotide in said mixture, whereby a change in said fluorescent signal is indicative of the presence of a primer extension product in said mixture.