Patent ID: 9316635
Filing Date: 2016-04-19
CPC Classification: G01N

Claim Text:
1. A particle and intracellular organelle alignment liquid (PIOAL) for use in a combined viscosity and geometric hydrofocusing analyzer, the PIOAL directing flow of a blood sample fluid of a given viscosity that is injected into a narrowing flowcell transition zone of the visual analyzer so as to produce a sample fluid stream enveloped by the PIOAL, the PIOAL comprising: a fluid having a higher viscosity than the viscosity of the blood sample fluid, a pH adjusting agent, and sodium sulfate, sodium chloride, Procaine HCl, potassium phosphate monobasic, sodium phosphate dibasic, disodium EDTA, and deioinzed water, wherein the pH adjusting agent is sodium hydroxide, wherein a viscosity hydrofocusing effect induced by an interaction between the PIOAL fluid and the sample fluid associated with the viscosity difference, in combination with a geometric hydrofocusing effect induced by an interaction between the PIOAL fluid and the sample fluid associated with the narrowing flowcell transition zone, is effective to provide a target imaging state in at least some of the plurality of particles at an imaging site of the visual analyzer while a viscosity agent in the PIOAL retains viability of cells in the sample fluid stream leaving structure and content of the cells intact when the cells extend from the sample fluid stream into the flowing sheath fluid, and wherein the viscosity agent of the sheath fluid comprises glycerol at a concentration between about 1 to about 50% (v/v).