Patent ID: 9523129
Filing Date: 2016-12-20
CPC Classification: C12Q

Claim Text:
1. A method for determining a clonotype profile of T cell receptors and/or B cell receptors of a subject, the method comprising the steps of: a) spatially isolating individual nucleic acid molecules, wherein the individual nucleic acid molecules are derived from nested sets of templates, wherein each of the nested sets of templates is generated from a nucleic acid in a sample comprising nucleic acids from T-cells and/or B-cells of the subject and contains a somatically rearranged region or a portion thereof, whereby each nested set of the nested sets of templates comprises a plurality of overlapping templates such that every template of the plurality of overlapping templates has a common end and a different end, and wherein each nested set is capable of producing a plurality of sequence reads each extending in the same direction and each starting from a different position on the nucleic acid from which the nested set was generated; b) sequencing the spatially isolated individual nucleic acid molecules, wherein the sequencing produces sequence reads with a sequence length of at least 30 nucleotides, and wherein each nested set is represented by a plurality of sequence reads; c) combining information from the plurality of sequence reads from each nested set to form a clonotype from each nested set, whereby the combining forms at least 1000 clonotypes from the nested sets of templates; and d) determining abundances of each of clonotype from each nested set to generate the clonotype profile.