Patent ID: 9304065
Filing Date: 2016-04-05
CPC Classification: B01L,C12P,C12Q,G01N

Claim Text:
1. A method for multiple single-cell capturing and processing using microfluidics, the method comprising: a) loading a plurality of cells into a microfluidic device; b) flowing the plurality of cells to a first capture configuration of the microfluidic device; c) capturing a first single cell from the plurality of cells in the first capture configuration, wherein the first capture configuration comprises one or more physical barriers configured to capture a single cell from the plurality of cells and wherein the first capture configuration is coupled to a first multi-chamber reaction configuration; d) flowing a first remaining plurality of cells from the plurality of cells through one or more first bypass channels to a second capture configuration wherein the second capture configuration comprises one or more physical barriers configured to capture a single cell from the plurality of cells and wherein the second capture configuration coupled to a second multi-chamber reaction configuration; e) capturing a second single cell from the first remaining plurality of cells in the second capture configuration; f) contacting the first and second single cells with a lysis reagent to release one or more cell constituents of each respective captured cell; g) flowing the one or more constituents of the first captured cell into the first multi-chamber reaction configuration and flowing the one or more constituents of the second captured cell into the second multi-chamber reaction configuration; and h) processing the one or more constituents of the first captured single cell in the first multi-chamber reaction configuration and processing the one or more constituents of the second captured single cell in the second multi-chamber reaction configuration to produce harvest products with respect to at least the first captured single cell and the second captured single cell, wherein the multi-chamber reaction configurations each comprise: wherein steps (a)-(h) are carried out in the microfluidic device.