Patent ID: 9353377
Filing Date: 2016-05-31
CPC Classification: C12N

Claim Text:
1. A method of producing multiple transgenic wheat plants from a single explant comprising: providing an explant presenting a plurality of meristems wherein said explant comprises a scutellar node; culturing said explant on a first multiple bud inducing media suitable for inducing production of a plurality of buds from at least one of said meristems; introducing exogenous DNA into at least one of said plurality of buds; removing said plurality of buds from said first media and transferring said plurality of buds to a second media suitable for induction of elongation of said buds into shoots; harvesting and transferring said shoots to a culture medium that promotes root development; and culturing said transferred shoots to produce multiple transgenic wheat plants, wherein the multiple bud inducing medium comprises a cytokinin and an auxin wherein the cytokinin is thidiazuron.