Patent ID: 9333249
Filing Date: 2016-05-10
CPC Classification: A61K,A61P,C07K,C12N,Y02A

Claim Text:
1. A method of producing a transfer vector comprising: inserting a fusion DNA sequence comprising (A) a DNA fragment of the DNA sequence encoding the amino acid sequence of a malaria antigen protein having immunogenicity; and (B) a DNA fragment of the DNA sequence encoding the amino acid sequence of a baculovirus gp64 protein that is a component of the viral particle, linked downstream of and under the control of a dual promoter comprising a polyhedrin promoter and a CAG promoter directly linked to each other, to produce a dual promoter expression cassette, (1) wherein the DNA fragment of (A) is a gene encoding an amino acid sequence consisting of 19-373 amino acid residues of SEQ ID NO: 104, and the DNA fragment of (B) is a gene encoding an amino acid sequence of SEQ ID NO: 114 (pCAP-PfCSP), (2) wherein the DNA fragment of (A) is a gene encoding an amino acid sequence consisting of 19-373 amino acid residues of SEQ ID NO: 104, and the DNA fragment of (B) is a gene encoding an amino acid sequence consisting of 447-492 amino acid residues of SEQ ID NO: 114 (pCAP-PfCSP/467), (3) wherein the DNA fragment of (A) is a gene encoding an amino acid sequence consisting of 205-373 amino acid residues of SEQ ID NO: 104, and the DNA fragment of (B) is a gene encoding an amino acid sequence of SEQ ID NO: 114 (pCAP-CO/205), (4) wherein the DNA fragment of (A) is a gene encoding an amino acid sequence consisting of 76-373 amino acid residues of SEQ ID NO: 104, and the DNA fragment of (B) is a gene encoding an amino acid sequence consisting of 447-492 amino acid residues of SEQ ID NO:114 (pCAP-CO/76/467), (5) wherein the DNA fragment of (A) is a gene encoding an amino acid sequence consisting of 76-373 amino acid residues of SEQ ID NO: 104, and the DNA fragment of (B) is a gene encoding an amino acid sequence of SEQ ID NO: 114 (pCAP-CO/76), or (6) wherein the DNA fragment of (A) is a gene encoding an amino acid sequence consisting of 1-397 amino acid residues of SEQ ID NO: 104, and the DNA fragment of (B) is a gene encoding an amino acid sequence of SEQ ID NO: 114 (pCAP-CO/full); and inserting said dual promoter expression cassette into an expression vector to produce the transfer vector.