Patent ID: 9499837
Filing Date: 2016-11-22
CPC Classification: C12N,C12Q,C12Y

Claim Text:
1. A method for making a targeted modification in a male fertility gene in the genome of a plant, said method comprising: (a) contacting at least one plant cell comprising, in a MS26 male fertility gene, a target sequence comprising SEQ ID NO.: 1 with an engineered meganuclease that is capable of inducing a double-strand break at the target sequence in the MS26 male fertility gene, wherein the engineered meganuclease is modified to specifically cut at the target sequence, and wherein the engineered meganuclease no longer cuts at its wild-type meganuclease target sequence; and (b) identifying at least one cell from step (a) comprising an alteration in its genome at the target sequence wherein the alteration is selected from the group consisting of (i) replacement of at least one nucleotide, (ii) a deletion of at least one nucleotide, (iii) an insertion of at least one nucleotide, and (iv) any combination of (i) through (iii); wherein the alteration of the male fertility gene is a null mutation.