Patent ID: 9382568
Filing Date: 2016-07-05
CPC Classification: C12Q

Claim Text:
1. A method for determining the resistance or sensitivity of a cell line or patient sample to a deoxyribonucleoside kinase-dependent drug, the method comprising the steps of: (i) treating a patient sample or cell line, or a portion thereof, with a deoxyribonucleoside kinase-dependent drug; (ii) lysing the cells of the patient sample or cell line from step (i); (iii) mixing a portion (1) of the cell lysate from (ii) with (A) a bioluminescent reporter bacteria incorporating a gene coding for a deoxyribonucleoside kinase and (B) a deoxyribonucleoside kinase transcription promoter, wherein the mixture of (iii) does not include a dephosphorylating agent; (iv) mixing a portion (2) of the cell lysate from step (ii) with (A) a bioluminescent reporter bacteria incorporating a gene coding for a deoxyribonucleoside kinase, (B) a deoxyribonucleoside kinase transcription promoter and (C) a dephosphorylating agent; and (v) measuring the bioluminescence of each of the mixtures from steps (iii) and (iv); and (vi) comparing the levels of bioluminescence of each of the mixtures from steps (iii) and (iv), wherein the comparative levels of bioluminescence of the two mixtures from steps (iii) and (iv) provide a measure of the resistance or sensitivity to the drug, wherein (A), (B) and (C) are three separate components.