Patent ID: 9447411
Filing Date: 2016-09-20
CPC Classification: C12N,C12Q

Claim Text:
1. A method of obtaining a single cell expression profile from a target mammalian cell, the method comprising: contacting a cellular sample comprising the target mammalian cell with a packaged viral barcoded trans-splicing library comprising a plurality of barcoded trans-splicing constructs under transduction conditions, wherein a barcoded trans-splicing construct comprises a trans-splicing element linked to a barcode element and the plurality of barcoded trans-splicing constructs comprises a set of constructs each having a distinct barcode element linked to a common trans-splicing element that is a spliceosome-recognized trans-splicing element comprising a splice acceptor site; and generating expression data from the resultant transduced target mammalian cell to obtain the single cell expression profile from the target mammalian cell, wherein the single cell expression profile comprises the expression level of 50 or more genes of interest.