Patent ID: 9388389
Filing Date: 2016-07-12
CPC Classification: A61K,C07K,C12N,C12Q,G01N

Claim Text:
1. A RNA comprising structural genes (Core, E1 and E2), p7 and non-structural gene NS2 from a human hepatitis C virus selected from the group consisting of genotypes 1a, 1b, 2a, 3a, 4a, 5a, 6a and 7a, non-structural genes NS3, NS4A, NS4B and NS5B from the human hepatitis C virus genotype 2a strain JFH1, an HCV 5′ UTR and an HCV 3′ UTR, and non-structural gene NS5A from the human hepatitis C virus genotype selected from the group consisting of 1a, 1b, 2a, 3a, 4a, 5a, 6a and 7a with the proviso that the NS5A gene from a genotype 2a strain is not JFH1, wherein the genotype of Core-NS2 correspond to that selected of the NS5A genotype, wherein the RNA is capable of infection in vitro, and wherein the corresponding cDNA sequence of said RNA comprises a sequence selected from the group consisting of SEQ ID NO:44, SEQ ID NO:46, SEQ ID NO:47, SEQ ID NO:49, SEQ ID NO:52, SEQ ID NO:54, SEQ ID NO:56, and SEQ ID NO:58 except for one or more adaptive mutations encoding amino acid substitutions selected from the group consisting of C1185S, Y1644H, E2264G, 12274N, C2422R, and C2439R.