Patent ID: 9446073
Filing Date: 2016-09-20
CPC Classification: A61K,C12N

Claim Text:
1. A method of producing a substantially pure in vitro cell culture of non-lineage committed precursor cells (n-LCP) isolated from the dental papillary annulus of teeth in any stage of development, comprising: (a) providing dental tissue in any stage of development from a mammal; (b) dissecting the dental tissue to isolate its dental papillary annulus; (c) culturing the annular tissue as adherent explants on collagen-coated and/or fibronectin-coated culture substrates to isolate n-LCPs characterized by the positive expression of one or more of the following markers: (d) culturing one or more of the isolated n-LCPs as adherent cells under suitable conditions to obtain a substantially pure population; and (e) expanding one or more of the isolated n-LCP populations as adherent cells by sub-culture or other suitable means.