Patent ID: 9339561
Filing Date: 2016-05-17
CPC Classification: A01K,A61K,C12N,C12Q,C12Y

Claim Text:
1. A polynucleotide encoding a biosensor polypeptide comprising a modified circularly permuted thermostable luciferase and a peptide linker linking the C-terminal portion of the circularly-permuted thermostable luciferase to the N-terminal portion of the same circularly-permuted thermostable luciferase, wherein the modified circularly-permuted thermostable luciferase is modified relative to an unmodified circularly-permuted thermostable luciferase by one or more amino acid substitutions or insertions, the peptide linker comprising a sensor region capable of interacting with a target molecule in a cell, wherein the modified circularly-permuted thermostable luciferase has an enhanced response after interaction of the biosensor with the target molecule relative to at least one of: i) the unmodified circularly-permuted thermostable luciferase in the presence of the target molecule, or ii) the modified circularly-permuted thermostable luciferase in the absence of the target molecule, wherein the modified circularly-permuted thermostable luciferase comprises a substitution of an amino acid at a position that corresponds to position 507 of SEQ ID NO:2 and wherein the encoded amino acid sequence of the modified thermostable luciferase has at least 90% amino acid sequence identity with the amino acid sequence of SEQ ID NO:2.