Patent ID: 9493811
Filing Date: 2016-11-15
CPC Classification: C12Q,G01N,Y02A

Claim Text:
1. An in vitro method for diagnosing, in a newborn, a vertically transmitted infectious disease caused by a pathogen, the method comprising steps of: identifying the pathogen; obtaining a blood sample from the newborn between the time of birth and the 9 isolating the pathogen-specific IgG3s present in the blood sample obtained from the newborn, the blood sample comprising pathogen-specific IgG3s of the newborn and pathogen-specific IgG3s of maternal origin transmitted to the newborn during pregnancy; submitting the isolated pathogen-specific IgG3s to an enzymatic digestion in the presence of the endoproteinase AspN and of trypsin in order to obtain a mixture of proteotypic peptides of IgG3; and detecting, by mass spectrometry, among the mixture of proteotypic peptides of IgG3, the presence of at least one peptide distinctive of G3m and/or IGHG3 alleles in order to determine the G3m and IGHG3 alleles of the pathogen-specific IgG3s present in the blood sample obtained from the newborn, wherein the at least one peptide distinctive of G3m and/or IGHG3 alleles belongs to the group consisting of the peptides of SEQ ID NOs: 1 to 32, and any combination thereof; comparing the G3m and IGHG3 alleles of the pathogen-specific IgG3s present in the blood sample obtained from the newborn to the G3m and IGHG3 alleles of the IgG3s present in a blood sample obtained from the mother; and detecting, and optionally quantifying, the pathogen-specific IgG3s of the newborn.