Patent ID: 9309552
Filing Date: 2016-04-12
CPC Classification: C12Q

Claim Text:
1. A method for specifically detecting and identifying Streptococcus agalactiae from among other bacteria species having esterase activity in a sample suspected of containing Streptococcus agalactiae , the method comprising: inoculating the sample on a reaction medium comprising: at least one synthetic esterase enzymatic substrate selected from the group consisting of halogenated indoxyloctanoate derivatives, halogenated indoxylnonanoate derivatives, and halogenated indoxyldecanoate derivatives that a synthetic non-esterase enzymatic substrate capable of being used by wherein: use the esterase substrate and not the non-esterase substrate; or do not use the esterase substrate and do not use the non-esterase substrates; or use both the esterase substrate and the non-esterase substrate; the esterase and non-esterase enzymatic substrates are present in the reaction medium at a concentration of 10 to 2000 mg/L; a bacteria exhibiting a detectable modified appearance attributable to the esterase enzymatic substrate at 18 hours after inoculation indicates that the colony is not of the species a presence of a colony that does not exhibit a detectable modified appearance attributable to the esterase enzymatic substrate at 18 hours of incubation and exhibits a detectable modified appearance attributable to the non-esterase enzymatic substrate indicates that the sample contains and the detectable modified appearance is a change of color of the colony that is visualized by the naked eye, or fluorescence of the colony.