Patent ID: 9410165
Filing Date: 2016-08-09
CPC Classification: C07K,C11D,C12N,C12P,C12Y,Y02E

Claim Text:
1. A nucleic acid construct comprising a polynucleotide comprising a nucleotide sequence that encodes a GH61 polypeptide having cellulolytic enhancing activity, wherein the polynucleotide is operably linked to one or more heterologous control sequences that direct the production of the GH61 polypeptide in an expression host, and wherein the GH61 polypeptide is selected from the group consisting of: (a) a GH61 polypeptide comprising an amino acid sequence having at least 95% identity to amino acids 20 to 258 of SEQ ID NO: 6; and (b) a GH61 polypeptide encoded by a polynucleotide which hybridizes under high stringency conditions with (i) nucleotides 126 to 978 of SEQ ID NO: 5, (ii) the cDNA sequence contained in nucleotides 126 to 978 of SEQ ID NO: 5, or (iii) a full-length complement of (i) or (ii), wherein high stringency conditions are defined as prehybridization and hybridization at 42° C. in 5× SSPE, 0.3% SDS, 200 μg/ml sheared and denatured salmon sperm DNA, and 50% formamide, and washing three times each for 15 minutes using 2× SSC, 0.2% SDS preferably at least at 65° C.