Patent ID: 9399658
Filing Date: 2016-07-26
CPC Classification: C07H,C12N

Claim Text:
1. A method of preparing an oligonucleotide of formula (I), wherein V V W W W R or wherein two R M X is NH, NR Z represents a capture tag which is a long-chain alkyl residue, a perfluoroalkyl entity, an azide or an alkynyl group, Y represents a bond or a linker connecting the capture tag to X, and ON represents an oligonucleotide comprising at least 4 nucleotide or nucleotide analogue building blocks, comprising the steps: with an oxidizing agent to obtain a compound of formula (IIb) (b) reacting a compound of formula (IIb) with a capture tag agent of formula (III), to obtain a reaction product comprising the oligonucleotide of formula (I), and (c) contacting the reaction product of step (b) with a capture reagent capable of interacting with the capture tag, wherein the contacting takes place under conditions which allow separation of the oligonucleotide (I) from other species contained in said reaction product.