Patent ID: 9383373
Filing Date: 2016-07-05
CPC Classification: G01N

Claim Text:
1. A method of determining a presence of an ergosterol-containing organism in a substrate, by detecting a presence of ergosterol in the substrate, the method comprising: isolating a sterol-containing portion of the substrate, by subjecting the substrate to extraction, using an organic hydrophobic solvent; contacting said sterol-containing portion with a compound capable of selectively forming a conjugate with ergosterol in said sterol-containing portion of the substrate, said compound being a [1,2,4]-triazole-3,5-dione (TAD); and determining a presence of said conjugate by a spectroscopic assay, said presence of said conjugate being indicative for the presence of ergosterol and thereby indicative for the presence of an ergosterol-containing organism in the substrate.