Patent ID: 9416426
Filing Date: 2016-08-16
CPC Classification: C12Q

Claim Text:
1. A method for detecting Chlamydia trachomatis in a biological sample comprising: providing a biological sample; contacting the biological sample with a substance comprising an oligonucleotide set that comprises at least one oligonucleotide probe that is detectably labeled and has a nucleotide sequence length of about 10 to about 50 and at least two oligonucleotide primers each having a nucleotide sequence length of about 10 to about 150, under conditions such that the probes and primers anneal to SEQ ID NO:2 at the location between about base pairs 1835-2176 of the pmpA gene; wherein the probe comprises a sequence selected from the group consisting of SEQ ID NO:12, SEQ ID NO:15 and oligonucleotide sequences that are at least 70% homologous to SEQ ID NO:12, and SEQ ID NO:15 and the primers comprise oligonucleotide sequences selected from the group consisting of SEQ ID NO:10, SEQ ID NO:11, SEQ ID NO:13, SEQ ID NO:14, SEQ ID NO:16 and oligonucleotide sequences that are at least 70% homologous to SEQ ID NO:10, SEQ ID NO:11, SEQ ID NO:13, SEQ ID NO:14 and SEQ ID NO:16; amplifying the target sequence between the two primers using polymerase chain reaction; and detecting the label as an indication of the hybridization of the probe set to the target sequence thereby indicating the presence or amount of