Patent ID: 9315788
Filing Date: 2016-04-19
CPC Classification: A61K,A61P,C07K,C12N,C12P

Claim Text:
1. A compact Transcription Activator-Like Effector Nuclease (TALEN) monomer comprising: (i) one core TALE scaffold comprising Repeat Variable Dipeptide regions (RVDs) having DNA binding specificity onto a specific double-stranded DNA target sequence of interest; and (ii) at least one I-TevI nuclease domain, wherein said I-TevI nuclease domain consists essentially of: wherein said I-TevI nuclease domain is capable of cleaving DNA a few base pairs away from said double-stranded DNA target sequence of interest when fused to the C-terminus or N-terminus of said core TALE scaffold from (i); wherein said compact TALEN monomer is assembled to bind said target DNA sequence and cleave double-stranded DNA without requiring dimerization.