Patent ID: 9290799
Filing Date: 2016-03-22
CPC Classification: C12Q,C40B,G01N

Claim Text:
1. A method of identifying one or more target nucleic acids, the method comprising: providing a sample containing nucleic acid molecules; mixing the sample with one or more captures and one or more probes to obtain a mixture, wherein (i) each capture is labeled with one or more detectable labels such that it is distinguishable from the other captures by its detectable labels, and contains a capture nucleic acid sequence complementary to a first target sequence in a target nucleic acid, and (ii) each probe is labeled with one or more detectable labels such that it is distinguishable from the other probes by its detectable labels, and contains a probe sequence that is complementary to a second target sequence in a target nucleic acid, whereby a combination of detectable labels identifies a target nucleic acid molecule; allowing the one or more captures and the one or more probes to hybridize to the nucleic acid molecules, thereby complexes each containing a target nucleic acid, a capture, and a probe are formed; and determining the detectable labels bound to the complexes by imaging the detectable labels separately to obtain individual images, whereby one or more target nucleic acids are identified; wherein the hybridization step is carried out in an emulsion.