Patent ID: 9516888
Filing Date: 2016-12-13
CPC Classification: A23C,A23L,A61K,A61P,C12N,C12Y

Claim Text:
1. An enzyme preparation comprising a mixture of isolated β-galactosidase enzymes obtained from Bacillus circulans, wherein the mixture of enzymes comprises enzymes selected from the group consisting of SEQ ID NO: 8, SEQ ID NO: 9, SEQ ID NO: 10, a polypeptide having at least 90% identity to SEQ ID NO: 8, a polypeptide having at least 90% identity to SEQ ID NO: 9, and a polypeptide having at least 90% identity to SEQ ID NO: 10, wherein SEQ ID NO: 8, SEQ ID NO: 9, and SEQ ID NO: 10 are c-terminus truncations of native β-galactosidase, and wherein the enzymes have decreased p-nitrophenyl-β-D-galactopyranoside (ONPG) hydrolyzing activity relative to the native β-galactosidase and/or increased lactose hydrolyzing activity relative to the native β-galactosidase.