Patent ID: 9499838
Filing Date: 2016-11-22
CPC Classification: A01K,A61K,C07K,C12N

Claim Text:
1. A method for producing a monoclonal antibody, which comprises: immunizing with a desired antigen, a human antibody-producing mouse which carries (1) a human artificial chromosome in which a SC20 chromosome vector is linked to an about 1 Mb to about 3.5 Mb fragment of human chromosome 22 comprising an unrearranged human antibody λ light chain locus, and (2) an unrearranged human antibody κ light chain locus retained on a KCo5 transgene or on fragment W23 of human chromosome 2 (hCF(2-W23)), wherein at least both alleles of endogenous heavy chain locus and κ light chain locus are disrupted or inactivated, and which expresses a human antibody heavy chain comprising a human antibody Ig γ isotype, a human antibody κ light chain, and a human antibody λ light chain; producing a hybridoma by fusing a mouse myeloma cell with the a spleen cell derived from the mouse; and thereby producing a monoclonal antibody comprising human immunoglobulin heavy chains and light chains against the antigen.