Patent ID: 9382528
Filing Date: 2016-07-05
CPC Classification: C12N,C12P,C12Y,Y02P

Claim Text:
1. An isolated cDNA encoding a nitrile hydratase protein having higher heat resistance and acylamide resistance than heat resistance and acylamide resistance of the wild type nitrile hydratase protein comprising (1) a β subunit amino acid sequence having at least 90% homology to the β subunit amino acid sequence set forth in SEQ ID NO: 2 wherein amino-acid residues corresponding to amino acid residues at positions 57, 107, 114, 167 and 219 in the amino-acid sequence of SEQ ID NO: 2 are substituted with amino-acid residues that are different than the amino acid residues at the corresponding positions in SEQ ID NO: 2, and (2) an α subunit amino acid sequence having at least 90% homology to the α subunit amino acid sequence of SEQ ID NO: 4, and (3) wherein said encoded nitrile hydratase protein comprises one or more further amino acid substitutions in either or both of said α and β subunit amino acid sequences at: