Patent ID: 9260752
Filing Date: 2016-02-16
CPC Classification: A61K,A61P,C12N,C12Q

Claim Text:
1. A method for detecting if two complexes are in proximity to one another comprising: contacting a first target nucleic acid with a first complex, wherein said first complex comprises a first site-directed polypeptide, a first modified nucleic acid-targeting nucleic acid comprising a first nucleic acid sequence complementary to the first target nucleic acid, and a first effector protein, wherein said first effector protein is adapted to bind to said first modified nucleic acid-targeting nucleic acid, and wherein said first effector protein comprises a first non-native sequence that comprises a first portion of a split system; contacting a second target nucleic acid with a second complex, wherein said second complex comprises a second site-directed polypeptide, a second modified nucleic acid-targeting nucleic acid comprising a second nucleic acid sequence complementary to the second target nucleic acid, and a second effector protein, wherein said second effector protein is adapted to bind to said second modified nucleic acid-targeting nucleic acid, and wherein said second effector protein comprises a second non-native sequence that comprises a second portion of a split system; and detecting an interaction between said first portion and said second portion, wherein said detecting indicates said first complex and said second complex are in proximity to one another.