Patent ID: 9334531
Filing Date: 2016-05-10
CPC Classification: C12N,C12Q,C12Y

Claim Text:
1. A method for nucleic acid amplification, comprising: a) distributing at least two different polynucleotide templates onto an array of reaction sites on a support wherein the array of reaction sites comprise the same first universal primer that does not include a template sequence by introducing a single one of each of said different polynucleotide templates into at least two different reaction sites of the array, wherein the polynucleotide templates each include the same first universal primer binding site, the same second universal primer binding site, and are present within a reaction mixture in a single continuous liquid phase; and b) forming at least two substantially monoclonal nucleic acid populations by amplifying, by partially denaturing and without compartmentalizing and within the same reaction mixture of step (a) in the continuous liquid phase, each of the single polynucleotides within said at least two reaction sites, wherein the amplifying is performed using a second universal primer in solution that can hybridize to the second universal primer binding site within the polynucleotide templates, and wherein said at least two reaction sites are in fluid communication with each other during the amplifying.