Patent ID: 9493735
Filing Date: 2016-11-15
CPC Classification: A61F,A61L,C12M,C12N

Claim Text:
1. A method of producing organized skeletal muscle tissue from precursor muscle cells, comprising: (a) providing a decellularized tissue support in a tissue media in a bioreactor, said support comprising mammalian precursor muscle cells; then (b) cyclically stretching and relaxing said support at least twice along a first axis during a first time period, wherein said first time period is from 2 to 30 minutes in duration; and then (c) maintaining said support in a substantially static position during a second time period, wherein said second time period is from 10 to 100 minutes in duration; and then (d) repeating steps (b) and (c) for a number of times sufficient to enhance the functionality of the muscle tissue or produce organized skeletal muscle tissue on said support from said precursor muscle cells, wherein the repeating steps (b) and (c) is carried out for a time of up to two weeks; wherein said organized skeletal muscle tissue is characterized by enhancing cellular organization, contractile function and tissue formation when implanted in vivo.