Patent ID: 9492572
Filing Date: 2016-11-15
CPC Classification: A61K,A61P,C07K,C12N,G01N

Claim Text:
1. A hetero-dimeric modified ubiquitin protein with binding capability to a non-natural target protein of human ubiquitin, comprising two ubiquitin monomers linked together in a head-to-tail arrangement, wherein both monomers of said hetero-dimeric protein are differently modified at least by substitutions of at least 5, 6, 7, or 8 amino acids corresponding to positions 2, 4, 6, 8, 62, 63, 64, 65, 66, and 68 of SEQ ID NO: 1, and wherein 2-15 amino acids are inserted in at least one ubiquitin monomer 0, 1, 2, or 3 amino acids distant from said amino acid substitutions corresponding to positions 2, 4, 6, 8, 62, 63, 64, 65, 66, and 68 of SEQ ID NO:1, and wherein said modified ubiquitin monomers have an amino acid identity to SEQ ID NO: 1 of at least 75% and said modified hetero-dimeric ubiquitin have a specific detectable binding affinity to said non-natural target protein of Kd=10