Patent ID: 9458497
Filing Date: 2016-10-04
CPC Classification: C12Q,G01N

Claim Text:
1. A method comprising: (a) providing an array comprising a solid surface with a plurality of nucleic acid probes at independently addressable locations, which plurality of nucleic acid probes comprises 5 or more different nucleotide sequences; (b) using the array to measure an amount of 5 or more amplicons corresponding to the 5 or more different nucleotide sequences in a single fluid volume during or after multiple amplification cycles to determine amplicon amount-amplification cycle values; and (c) using the amplicon amount-amplification cycle values to determine a presence or amount of the 5 or more nucleotide sequences in a sample, wherein the amount of the 5 or more amplicons is measured by measuring kinetics of binding of the 5 or more amplicons to nucleic acid probes.