Patent ID: 9416398
Filing Date: 2016-08-16
CPC Classification: C12Q

Claim Text:
1. An automated process for amplifying and determining the quantity of at least a first and a second target nucleic acid that may be present in at least one fluid sample, said process comprising the steps of: a) separately incubating in at least two reaction vessels said target nucleic acids with a solution comprising amplification reagents, and oligonucleotides specific for said first or second target nucleic acid, wherein said amplification reagents comprise a polymerase with reverse transcriptase activity, for a period of time and under conditions suitable for transcription of RNA by said polymerase with reverse transcriptase activity to occur, b) separately incubating in said at least two reaction vessels said target nucleic acids with said solution comprising amplification reagents, and oligonucleotides specific for said first or second target nucleic acid for a period of time and under conditions sufficient for an amplification reaction of said first and second target nucleic acid to occur, and c) determining the quantity of said first and second target nucleic acid by referencing to an external calibration or by implementing an internal quantitative standard; wherein said solutions in said first and in said second reaction vessels comprise the same concentration of amplification reagents, and wherein oligonucleotides for said first target nucleic acid are present in the first of said at least two reaction vessels and absent in the second of said at least two reaction vessels, and oligonucleotides for said second target nucleic acid are present in the second of said at least two reaction vessels and absent in the first of said at least two reaction vessels, wherein said first target nucleic acid is an RNA target nucleic acid and said second target nucleic acid is a DNA target nucleic acid, and wherein the conditions for transcription in step a) and for amplification in step b) are identical for said at least first and second target nucleic acids comprised in said first and second reaction vessels.