Patent ID: 9382533
Filing Date: 2016-07-05
CPC Classification: C12N,C12Q,G01N

Claim Text:
1. A method for identifying or producing an aptamer to a target in a tissue or cell sample, the method comprising: a) contacting a candidate mixture of nucleic acids with the tissue or cell sample, wherein at least one nucleic acid of the candidate mixture comprises at least one C-5 modified pyrimidine, and wherein nucleic acids having affinity to the target bind the target and form nucleic acid-target complexes; b) partitioning nucleic acid-target complexes from free nucleic acids in the candidate mixture; c) identifying nucleic acids that bind to the target in the tissue or cell sample, thereby identifying or producing an aptamer to a target in a tissue or cell sample; and wherein, the at least one C-5 modified pyrimidine improves the off-rate of the nucleic acid compared to the nucleic acid without the at least one C-5 modified pyrimidine.