Patent ID: 9249440
Filing Date: 2016-02-02
CPC Classification: C12N,C12P

Claim Text:
1. A genetically modified microbe comprising four exogenous polynucleotides, wherein the exogenous polynucleotides each encode a subunit, wherein a first subunit comprises an amino acid sequence, and the amino acid sequence of the first subunit and the amino acid sequence of SEQ ID NO:6 have at least 80% identity, wherein a second subunit comprises an amino acid sequence, and the amino acid sequence of the second subunit and the amino acid sequence of SEQ ID NO:8 have at least 80% identity, wherein a third subunit comprises an amino acid sequence, and the amino acid sequence of the third subunit and the amino acid sequence of SEQ ID NO:2 have at least 80% identity, wherein a fourth subunit comprises an amino acid sequence, and the amino acid sequence of the fourth subunit and the amino acid sequence of SEQ ID NO:4 have at least 80% identity, and wherein the four subunits form a tetrameric polypeptide having hydrogenase activity, wherein the four subunits are expressed during growth in anaerobic conditions.