Patent ID: 9474807
Filing Date: 2016-10-25
CPC Classification: A61K,A61P,C07K,C12N,C12Y

Claim Text:
1. A non-cytotoxic protein conjugate for inhibition or reduction of exocytic fusion in a nociceptive sensory afferent cell, comprising: (i) a Targeting Moiety (TM), wherein said TM binds to a Binding site on a nociceptive sensory afferent cell, wherein said Binding site undergoes endocytosis to be incorporated into an endosome within the nociceptive sensory afferent cell, and wherein the TM is dynorphin; (ii) a non-cytotoxic protease or a fragment thereof, wherein the non-cytotoxic protease or fragment thereof cleaves a protein of the exocytic fusion apparatus of said nociceptive sensory afferent cell and wherein the non-cytotoxic protease or fragment thereof is a clostridial neurotoxin; and (iii) a Translocation Domain, wherein the Translocation Domain translocates the non-cytotoxic protease or fragment thereof from within the endosome, across the endosomal membrane, and into the cytosol of the nociceptive sensory afferent cell and wherein the Translocation Domain is a botulinum H wherein said conjugate comprises an amino acid sequence selected from the group consisting of SEQ ID NOs: 1 10-119, 141 and 152-162.