Patent ID: 9267953
Filing Date: 2016-02-23
CPC Classification: G01N

Claim Text:
1. A method for determining an analyte in a sample wherein the analyte is an antigen-specific antibody of the immunoglobulin class G (IgG), said method comprising the steps of: (a) contacting the sample with a first analyte-specific receptor, said receptor comprising an antigen comprising at least two epitopes for the analyte, and a second analyte-specific receptor, said receptor comprising an antibody having low affinity and high avidity for the analyte, such that binding of at least two of the analyte molecules to the first receptor forms an arrangement and selective binding of the second receptor to the arrangement forms an immune complex, wherein one of the two receptors is bound to a solid phase or is capable of binding to a solid phase and the other receptor carries a signal-generating group or is capable of binding to a signal-generating group, and (b) determining the presence or/and the amount of the analyte by detecting the signal-generating group on the solid phase while not detecting either free IgG that is non-specific for the antigen or free, uncomplexed Ig that is specific for the antigen,