Patent ID: 9523112
Filing Date: 2016-12-20
CPC Classification: C12Q

Claim Text:
1. A method for reducing the duration of a microbial growth inhibition test, that comprises an indicator, for determining the presence or absence of a beta-lactam antibiotic, a tetracycline antibiotic, a sulfonamide antibiotic, an aminoglycoside antibiotic or a quinolone antibiotic in a sample, the method comprising: (a) contacting a sample with a test organism, (b) growing the test organism in the presence of the indicator by adding nutrients, and/or raising the temperature, and/or providing for a pH-value at which the test organism is able to grow, (c) detecting an amount of growth of the test organism by observing the presence or absence of a change of the indicator, wherein lack of growth of the test organism is determined by absence of a change of the indicator, and wherein lack of growth reflects a presence of a beta-lactam antibiotic, a tetracycline antibiotic, a sulfonamide antibiotic, an aminoglycoside antibiotic and/or a quinolone antibiotic in the sample, wherein between 50 and 20,000 units/ml of a beta-lactamase is added to the test organism before the test is started, and/or added to the sample before, during or after the sample is contacted with the test organism and wherein the beta-lactamase reduces the duration of the microbial growth inhibition test compared to the duration of the microbial growth inhibition test performed without beta-lactamase.