Patent ID: 9273327
Filing Date: 2016-03-01
CPC Classification: A61K,C12N,Y02A

Claim Text:
1. A method for inserting a nucleic acid sequence that encodes a foreign peptide into a poxvirus genome, said method comprising inserting the nucleic acid sequence that encodes the foreign peptide at a position downstream of a poxvirus promoter of an open reading frame (ORF) non-essential to the viability of a poxvirus, wherein said ORF corresponds to modified vaccinia virus Ankara (MVA) ORFs 176R, 041L, 005R, and 050L, or wherein said ORF corresponds to the orthologous vaccinia virus ORFs B8R, F11L, C11R, and E3L; wherein following said insertion, (i) the nucleic acid that encodes the foreign peptide is operably-linked to the poxvirus promoter of said non-essential ORF and expression of said nucleic acid is driven by said poxvirus promoter; and (ii) translation of the foreign peptide is initiated at an ATG start codon located at the same position as the ATG start codon of said non-essential ORF.