Patent ID: 9506119
Filing Date: 2016-11-29
CPC Classification: C12Q

Claim Text:
1. A method of determining clonotypes of an immune repertoire, the method comprising the steps: (a) attaching sequence tags to molecules of recombined nucleic acids of I-cell receptor genes or immunoglobulin genes of T-cells and/or B-cells from a sample comprising cells and/or B-cells to form tag-molecule conjugates, wherein substantially every molecule of the tag-molecule conjugates has a unique sequence tag; (b) amplifying the tag-molecule conjugates; (c) sequencing by synthesis the tag-molecule conjugates to produce sequence reads comprising at least 1000 clonotypes per run, wherein each sequence read comprises a sequence tag portion and a clonotype portion; and (d) aligning like sequence tag portions of the sequence reads to determine a clonotype sequence from corresponding clonotype portions of the aligned sequence reads.