Patent ID: 9285367
Filing Date: 2016-03-15
CPC Classification: G01N

Claim Text:
1. A reagent for measurement of CD4 positive lymphocytes in whole blood using flow cytometry, the reagent consisting essentially of: a) 7-aminoactinomycin D (7-AAD) configured to discriminate between erythrocytes and leucocytes; b) CD4 monoclonal antibody conjugated to a fluorescent label; and, c) CD14 monoclonal antibody conjugated to a fluorescent label; wherein the CD4 monoclonal antibody is produced by MT4 hybridoma clone of which is deposited with the DSMZ under Accession Number DSM ACC3178 and the CD14 monoclonal antibody is produced by MT14/3 hybridoma clone of which is deposited with the DSMZ under Accession Number DSM ACC3185; wherein the CD4 monoclonal antibody and the CD14 monoclonal antibody are differentially labeled using fluorescein isothiocyanate (FITC) and phycoerythrin (PE); and, wherein the reagent is configured to simultaneously detect and enumerate CD4 positive lymphocytes in a lymphocyte population while reducing contamination of monocytes present in the whole blood sample using flow cytometric analysis.