Patent ID: 9340770
Filing Date: 2016-05-17
CPC Classification: C12N

Claim Text:
1. A method for achieving stable, long-term culture and expansion of human pluripotent stem cells or human multipotent stem cells in suspension culture in vitro, the method comprising: (i) attaching human pluripotent stem cells or human multipotent stem cells to a plurality of microcarriers to form microcarrier-stem cell complexes, wherein the surface of the microcarriers is coated in a matrix comprising an extracellular matrix component; (ii) culturing the microcarrier-stem cell complexes in suspension culture; (iii) passaging the cultured cells from (ii); and (iv) repeating steps (i)-(iii) through at least 7 passages, wherein stem cells in the culture after step (iv) are respectively pluripotent or multipotent, thus achieving stable, long-term culture and expansion of human pluripotent stem cells or human multipotent stem cells in suspension culture in vitro.