Patent ID: 9417234
Filing Date: 2016-08-16
CPC Classification: B05D,C12N,C12Q,G01N

Claim Text:
1. A method for forming a sensor for one or more analytes, comprising: a) adsorbing binary components comprised of 3-mercaptopropionic acid (MPA) and (1-mercapto-11-undecyl)tri(ethylene glycol) (PEG3) on a substrate; b) reductively desorbing MPA from the substrate of step a); c) forming a dithiobis-N-succinimidyl propionate (DTSP) layer on the substrate of step b); d) immobilizing at least one type of aptamer on the substrate of step c); and, e) removing unbound aptamer from the PEG3 on the substrate of step d), thus leaving aptamer attached to the DTSP layer of the substrate.