Patent ID: 9388384
Filing Date: 2016-07-12
CPC Classification: A61K,A61P,C12N

Claim Text:
1. A method for producing mesenchymal stem cells from human pluripotent stem cells, the method comprising: a) culturing the human pluripotent stem cells in an embryonic stem cell culture medium free from bFGF for 14 days to form embryonic bodies, wherein the embryonic body cells express one or more marker(s) selected from brachyury, BMPR, and Sox17; b) attaching the embryonic bodies of step a) to a culture dish and culturing the embryonic bodies comprised of embryonic body cells in Dulbecco's Modified Eagle's Medium (DMEM) containing fetal bovine serum (FBS) to induce differentiation of the embryonic body cells into mesenchymal stem cells; and c) maintaining and culturing the mesenchymal stem cells of step b) using a culture medium containing human epidermal growth factor (hEGF), vascular endothelial growth factor (VEGF), human fibroblast growth factor-basic (hFGF-B), insulin-like growth factor (IGF-1), hydrocortisone, and ascorbic acid, wherein the mesenchymal stern cells of steps b) and c) express one or more markers selected from the group consisting of CD29, CD44, CD73, CD90 and CD105, and wherein the mesenchymal stem cells of step c) are capable of being differentiated into cells selected from the group consisting of adipocytes, osteocytes, chondrocytes, myocytes, neurocytes, and cardiomyocytes.