Patent ID: 9458223
Filing Date: 2016-10-04
CPC Classification: A61K,A61P,C07K

Claim Text:
1. A polypeptide comprising a modified von Willebrand Factor (VWF), wherein the amino acid sequence of the modified VWF is identical to a human VWF sequence except for a modified D′ domain, wherein the modified D′ domain has at least one mutation relative to the amino acid sequence of positions 764 to 865 of SEQ ID NO:2, wherein the at least one mutation within the D′ domain comprises at least one amino acid substitution at a position selected from the group consisting of 779, 781, 793, 794, 796, 798, 802, 818, 819, 825, 835, 838 and 853 of the VWF amino acid sequence as shown in SEQ ID NO:2, and wherein the binding affinity of the polypeptide comprising the modified VWF to Factor VIII (FVIII) is higher than that of a reference human VWF polypeptide, wherein the amino acid sequence of the reference polypeptide comprises positions 764 to 865 of SEQ ID NO:2.