Patent ID: 9353417
Filing Date: 2016-05-31
CPC Classification: C12Q

Claim Text:
1. A method of diagnosing large granular lymphocyte leukemia in a patient comprising: isolating RNA from a sample of the patient; generating cDNA from the isolated RNA; hybridizing a gene-specific probe selected from the group consisting of SEQ NO: 8 and SEQ ID NO: 9 to the cDNA wherein the probe is specific for detecting polynucleotide sequences encoding at least one granzyme H variant or fragment thereof having a sequence selected from the group consisting of SEQ ID NO: 4 and SEQ ID NO:5; determining an expression level of the at least one granzyme H variant in the sample from the patient; comparing the expression level of the at least one granzyme H variant from the patient to a predetermined control expression level; wherein an overexpression of the at least one granzyme H variant in the sample as compared to the control expression level is indicative of a diagnosis of large granular lymphocyte leukemia.