Patent ID: 9513196
Filing Date: 2016-12-06
CPC Classification: B01L,C12N,G01N

Claim Text:
1. A method of purifying DNA in a sample in a microfluidic device comprising the steps of: (a) mixing the sample and a lysis buffer in a mixing region of a microfluidic device; (b) selectively lysing the cellular membranes of cells in the sample without lysing the nuclear membranes of cells in a cell lysing region of the microfluidic device to produce intact nuclei from the cells; (c) flowing a sample flow containing the intact nuclei into a cell trapping region and trapping the intact nuclei using a filter in the cell trapping region of the microfluidic device while flowing a cross-flow buffer and other components of the sample across the sample flow and through the filter into a waste collection region of the microfluidic device; (d) lysing the intact nuclei trapped by the filter; (e) releasing the DNA from the lysed nuclei; and (f) collecting the released DNA in a DNA collection region of the microfluidic device.