Patent ID: 9267941
Filing Date: 2016-02-23
CPC Classification: G01N,Y10S

Claim Text:
1. A method for identifying the presence of an antigen or an antibody in a sample, the method comprising: contacting the sample with a complementary capture antibody or a capture antigen in an aqueous solution, specifically binding the antigen or the antibody to the complementary capture antibody or capture antigen, respectively; and detecting the presence of the antigen or antibody bound to the complementary capture antibody or capture antigen with a detector antibody that binds to either (a) the antigen, which is bound to the complementary capture antibody, or (b) the antibody, which is bound to the capture antigen, the sample, the capture antibody or capture antigen, the aqueous solution, and the detector antibody forming a matrix, the matrix comprising a first protein that binds to said capture antibody, capture antigen, detector antibody, antigen or antibody in the sample to be detected with a K said aqueous solution comprising: a) a buffer to control pH; b) a compound A selected from the group consisting of polyalkylene glycol, polypropylene glycol, propylene glycol, polyethylene glycol, ethylene glycol, monosaccharides, disaccharides, trisaccharides, saccharose, mannose, trehalose, polyol, glycerol and mixtures thereof; and c) a non-ionic detergent; wherein the concentration of compound A is from 2.0-15.0% and the concentration of the non-ionic detergent is from 0.1-1.0%, wherein the concentration of compound A is in terms of % (v/v) when compound A is a liquid or the concentration of compound A is in terms of % (w/v) when compound A is a solid wherein the ratio of the non-ionic detergent to the compound A is from 1:15 to 1:25: wherein the non- ionic detergent is a compound selected from the group consisting of: a) a substituted phenyl residue having substituents R b) wherein n, x, y and z together is an integer of 5 to 40, R is a fatty acid residue; c) Dodecylpoly(ethyleneglycolether) d) 1-O-n-Octyl-β-D-glucopyranoside (n-Octylglucoside); and e) 1-O-n-Dodecyl-β-D-glucopyranosyl (1-4)alpha-D-glucopyranoside; wherein said aqueous solution reduces the binding of said first protein or said second protein in the sample to any one or more of the following: complementary capture antibody or capture antigen or detector antibody or the antigen or the antibody in the sample to be detected, thereby reducing interference by the first protein or the second protein with the specific binding of said antigen or antibody to the complementary capture antibody or capture antigen or detector antibody, compared to conducting said specific binding in the absence of said aqueous solution.