Patent ID: 9297029
Filing Date: 2016-03-29
CPC Classification: C12N,C12P,C12Y,Y02E,Y02P

Claim Text:
1. A method comprising: a) providing a recombinant yeast host cell that expresses an engineered biosynthetic isobutanol pathway, wherein the engineered biosynthetic isobutanol pathway comprises an acetolactate synthase (ALS) enzyme, a ketol-acid reductoisomerase (KARI) enzyme, a dihydroxy-acid dehydratase (DHAD) enzyme, a branched chain keto acid decarboxylase enzyme (DC), and an alcohol dehydrogenase (ADH) enzyme, each of which is encoded by a heterologous gene that lacks a mitochondrial targeting sequence, and wherein the yeast host cell is provided in a growth phase; b) growing the yeast host cells in fermentation medium, whereby isobutanol is bioproduced; and c) recovering the bioproduced isobutanol; wherein the yeast host cells are capable of producing 7- to 8-fold more isobutanol when grown on glucose under aerobic conditions compared to a recombinant yeast host cell lacking said engineered biosynthetic isobutanol pathway.