Patent ID: 9310370
Filing Date: 2016-04-12
CPC Classification: C12Q,G01N,G16B

Claim Text:
1. A method for detecting gastric cancer, comprising: (i) providing a sample of serum from a human being suspected of having gastric cancer; (ii) providing samples of serum from a group of control human beings not having gastric cancer; (iii) detecting the levels of zymogen granule protein 16 (“ZG16”) and mucin 17 (“MUC17”) in said sample in step (i) using rolling circle amplification; (iv) detecting the levels of a gastric cancer markers (“GTM”) zymogen granule protein 16 (“ZG16”) and MUC17 in said sample in step (ii) using rolling circle amplification, and normalizing expression data; (v) normalizing expression data obtained in step (iii) using quintile normalization; and (vi) comparing the expression of ZG16 and MUC17 in said sample in step (i) with the expression of ZG16 and MUC17 in step (ii), wherein an increase of normalized ZG16 expression and an increase of normalized MUC17 expression in the sample of serum from said human being compared to the normalized expression of ZG16 and MUC17 in a group of control samples of serum is indicative of gastric cancer.