Patent ID: 9528090
Filing Date: 2016-12-27
CPC Classification: C12N

Claim Text:
1. An in vitro cell culture comprising an isolated population of cells and a culture medium suitable for differentiating pluripotent stem cells, wherein greater than 85% of the cells are definitive endoderm cells, wherein said population of cells is obtained by differentiating in vitro pluripotent stem cells, wherein the culture medium lacks serum and is supplemented with BSA, GDF-8, 14-Prop-2-en-1-yl-3,5,7,14,17,23,27-heptaazatetracyclo[19.3.1.1˜2,6˜0.1˜8,12˜]heptacosa-1(25),2(27),3,5,8(26),9,11,21,23-nonaen-16-one and a factor selected from the group consisting of insulin and from about 1 ng/ml to about 50 ng/ml of IGF-1, and wherein said definitive endoderm cells express CXCR4 and do not express CD9.