Patent ID: 9334476
Filing Date: 2016-05-10
CPC Classification: A61L,C12M,C12N

Claim Text:
1. A method for growing and differentiating a neural cell or tissue in a subject comprising the steps of (a) isolating at least one differentiable neural cell from a donor subject; (b) preparing a three-dimensional matrix of electrospun fibers formed of a biocompatible synthetic permanently piezoelectric polymeric material to form a cell scaffold; (c) annealing, either thermally or chemically, the electrospun fibers, wherein after annealing the fibers demonstrate enhanced piezoelectric characteristics, crystal organization or a combination of both; (d) seeding the cell scaffold with the isolated differentiable neural cell; (e) growing the isolated differentiable neural cell on the cell scaffold ex vivo or in vitro; and (f) implanting the scaffold comprising the differentiable neural cell, wherein the differentiable neural cell differentiates into a mature neural cell phenotype on the scaffold.