Patent ID: 9499813
Filing Date: 2016-11-22
CPC Classification: C12N,C40B

Claim Text:
1. A method of amplifying a library of replicable bacteriophage clones while maintaining diversity of the library, the method comprising: a. distributing a library of replicable bacteriophage clones comprising a plurality of distinguishable replicable bacteriophage clones into a plurality of monodisperse microfluidic droplets-such that substantially no more than one replicable bacteriophage clone is contained in each of the monodisperse microfluidic droplets and wherein each of the monodisperse microfluidic droplets further comprises at least one bacterial cell capable of supporting replication of the respective replicable bacteriophage clones; and b. culturing the library of step (a) for a sufficient period of time such that the bacterial cell in each of the monodisperse microfluidic droplets replicates to reach substantially the same number therein, and wherein the library of the replicable bacteriophage clones is amplified when the replicable bacteriophage clones present in the respective monodisperse microfluidic droplets-infect the replicated bacterial cells therein; wherein the replicable bacteriophage clones in the library have different amplification rates, and wherein the ratio of bacteriophage clones of different amplification rates is maintained upon amplification thereby maintaining diversity of the library of replicable bacteriophage clones upon amplification.