Patent ID: 9388457
Filing Date: 2016-07-12
CPC Classification: C12Q,C40B

Claim Text:
1. A method for amplifying a plurality of target nucleic acid sequences from an un-amplified nucleic acid sample and analyzing the amplified target sequences, said method comprising: (a) fragmenting the un-amplified nucleic acid sample to obtain un-amplified target nucleic acid fragments; (b) mixing the un-amplified target nucleic acid fragments obtained in (a) with an array of probes arranged in features, wherein a feature comprises multiple copies of a target specific probe, so that un-amplified target nucleic acid fragments hybridize to complementary target specific probes to form probe:target complexes; (c) extending the probes in the probe:target complexes using a polymerase, wherein the un-amplified target nucleic acid fragment is used as template for extension of the probe and wherein an extended probe is generated; (d) removing the un-amplified target nucleic acid fragment from the extended probe by denaturation; (e) generating a plurality of complementary copies of the extended probe, wherein said copies are not covalently attached to the surface of the solid support; (f) allowing the copies to hybridize to the complementary target specific probes in the same feature; and (g) analyzing the copies, wherein the plurality of target nucleic acid sequences comprises between 100 and 100,000 different target nucleic acid sequences.