Patent ID: 9492527
Filing Date: 2016-11-15
CPC Classification: A61K,C12N,C12Y

Claim Text:
1. A method of inducing an immune response against a Human Papilloma Virus (HPV) antigen-expressing tumor or a HPV antigen-expressing cancer in a subject, said method comprising the step of administering to the subject a recombinant Listeria strain auxotrophic for D-alanine synthesis, comprising a mutation in a D-alanine racemase gene and in a D-amino acid transferase gene in the chromosome of said recombinant Listeria , wherein said Listeria further comprises a plasmid, wherein the plasmid comprises: a first nucleic acid sequence encoding a polypeptide, wherein said polypeptide comprises a heterologous antigen, and a second nucleic acid sequence encoding a D-alanine racemase enzyme, wherein said plasmid does not confer antibiotic resistance upon said recombinant whereby said D-alanine racemase gene complements said mutation in said chromosome of said recombinant