Patent ID: 9506054
Filing Date: 2016-11-29
CPC Classification: C07K,C12N

Claim Text:
1. A method for acquiring a heat-stable antibody-displayed phage, the method comprising steps of: (a) supplying an antibody-displayed phage library aqueous solution containing plural types of antibody-displayed phages to a support comprising a polypeptide on the surface thereof, so as to bind the plural types of the antibody-displayed phages to the polypeptide specifically; wherein the polypeptide consists of an amino acid sequence represented by SEQ ID NO: 01; each antibody-displayed phage comprises proteins containing a phagemid vector in the inside thereof; each antibody-displayed phage comprises an antibody fragment on the external surface thereof; and the phagemid vector has a gene sequence coding for the antibody fragment; (b) heating the support to the temperature of not less than 37 degrees Celsius and not more than 70 degrees Celsius, so as to release a portion of the antibody-displayed phages from the support and so as to leave the other antibody-displayed phages on the support selectively; and (c) collecting the other antibody-displayed phages which has been left on the support selectively in the step (b) so as to obtain the heat-stable antibody-displayed phage.