Patent ID: 9290787
Filing Date: 2016-03-22
CPC Classification: A23L,A23V,A61K,A61P,C12P

Claim Text:
1. A method of producing and purifying β-carotene by Blakeslea trispora fermentation, comprising the following steps: a) separately inoculating strains of b) culturing and propagating spores of the fresh spore suspension in a seeding tank at pH=6.3˜6.8 and the cultivation temperature of 25-30° C. to seeds for fermentation, wherein the seed medium comprises corn starch, dextrose, vegetable oil, thiamine hydrochloride, magnesium sulfate and emulsifier; c) inoculating the seeds for fermentation in the seeding tank of step b) onto a fermenter and then fermenting so as to produce a fermentation liquid at pH=6.5˜6.8 and the cultivation temperature of 27-30° C.; wherein the medium is high-speed sheared and emulsified by a colloidal mill before inoculating, the medium of the fermenter comprises corn starch, corn syrup, meal bread flour, vegetable oil, dipotassium hydrogen phosphate, magnesium sulfate, thiamine hydrochloride, and/or emulsifier; d) adjusting the fermentation liquid of step c) to be pH=8.0˜8.5 by using an organic or inorganic base, and then filtrating so as to obtain wet mycelia; e) treating the wet mycelia of step d) with hydrophobic non-polar organic solvent at 0° C.˜60° C., extracting and continuously filtering so as to obtain a wet mycelia without fat-soluble impurities; f) mixing the wet mycelia of step e) with an organic solvent of esters at the temperature from 10° C. to a boiling point of the solvent, and then concentrating to dryness in vacuum at the temperature ≦40° C. to obtain a concentrated solution; and g) adding a C1˜C6 saturated monohydric alcohol to the concentrated solution at 10° C.˜80° C., filtering and crystallizing so as to obtain pure β-carotene.