Patent ID: 9333251
Filing Date: 2016-05-10
CPC Classification: A61K,A61P,C12N,Y02A

Claim Text:
1. An immunoprotective composition comprising an attenuated bacterium expressing a first antigen useful for inducing an immunoprotective response against Shigella sonnei ( S. sonnei ) O-polysaccharide and a second antigen useful for inducing an additional immunoprotective response against S. sonnei and/or at least one bacterial strain selected from the group consisting of Shigella flexneri, Shigella dysenteriae, Shigella boydi, Salmonella typhi, Escherichia coli, Vibrio cholerae , and Yersinia , the second antigen comprising a heterologous surface protein antigen expressed in bacteria selected from the group consisting of S. sonnei, Shigella flexneri, Shigella dysenteriae, Shigella boydi, Salmonella typhi, Escherichia coli, Vibrio cholerae , and Yersinia, wherein the first antigen is the O-polysaccharide produced from enzymes encoded by an expression cassette comprising a polynucleotide fragment encoding the genes wbgT, wbgU, wzx, wzy, wbgV, wbgW, wbgX, wbgY, and wbgZ isolated from the wherein the expression cassette does not include sequences that naturally flank the rfb/rfc gene cluster, and wherein the second antigen is a heterologous surface protein antigen encoded by genes carried by the at least one bacterial strain.