Patent ID: 9420148
Filing Date: 2016-08-16
CPC Classification: B01L,C12M,C12N,G02B,G06T,H04N

Claim Text:
1. An apparatus for isolating individual target cells, the apparatus comprising: a body structure comprising a main channel, a collection channel, and a waste channel fluidly coupled at a first fluid junction, the body structure further comprising a plurality of trapping channels intersecting the collection channel, each of the trapping channels having a diameter at a location adjacent to the intersection of the trapping channel with the collection channel that is less than a diameter of an individual target cell; an imaging system configured to image each of a plurality of individual target and non-target cells within the main channel, thereby producing imaging data; a processor configured to perform real-time, multivariate analyses of the imaging data; a directing system configured to direct the individual target cells; and a pressure source in fluid communication with one or more of the collection channel, and the waste channel; wherein the pressure source is operable to apply a pressure selected from the group consisting of a positive pressure on the collection channel, a negative pressure on the waste channel, and a combination of the positive pressure and the negative pressure, the pressure having a first pressure and a second pressure greater than the first pressure.