Patent ID: 9322036
Filing Date: 2016-04-26
CPC Classification: C07K,C12N,C12P

Claim Text:
1. A method of inactivating an endogenous cellular FUT8 gene in an isolated cell, the method comprising: introducing, into an isolated cell, a first nucleic acid encoding a first polypeptide, wherein the first polypeptide comprises: (a) a first zinc finger DNA-binding domain, wherein the DNA-binding domain comprises four or five zinc finger recognition regions ordered F1 to F4, F1 to F5 or F1 to F6 from N-terminus to C-terminus, and wherein F1 to F4, F1 to F5 or F1 to F6 comprise amino acid sequences selected from the group consisting of: (b) a first cleavage domain; such that the polypeptide is expressed in the cell, whereby the polypeptide binds to the target site and cleaves the FUT8 gene; and introducing a nucleic acid encoding a second polypeptide, wherein the second polypeptide comprises: (i) a second zinc finger DNA-binding domain that is engineered to bind to a second target site in the FUT8 gene; and (ii) a second cleavage domain; such that the second polypeptide is expressed in the cell, whereby the first and second polypeptides bind to their respective target sites, dimerize and cleave the FUT8 gene, thereby inactivating the FUT8 gene.