Patent ID: 9329169
Filing Date: 2016-05-03
CPC Classification: G01N

Claim Text:
1. A method for comparing the relative abundance of one or more glycans of interest in a modulated biological sample compared to a control sample comprising: culturing a first biological sample to be modulated in a first culture medium further comprising culturing a control sample in a second culture medium identical to said first culture medium further comprising modulating said first sample during culturing; processing said first sample and said control sample to extract proteins and/or lipids therefrom; separating said proteins and/or lipids from said samples after said processing step; optionally digesting said proteins into a plurality of peptides; optionally releasing glycans from said proteins, peptides and/or lipids and separating said released glycans from said proteins, peptides and/or lipids; subjecting said proteins, said peptides, said lipids and/or said optionally released glycans to mass spectroscopy to develop a mass spectrum; and determining the relative abundance of said glycans of interest from each sample based upon said mass spectrum.