Patent ID: 9255928
Filing Date: 2016-02-09
CPC Classification: G01N

Claim Text:
1. A method for determining presence in a population of molecules of at least one first member of a binding pair having the capacity to bind specifically to at least one second member of said binding pair, the method comprising: (i) fractionating the population of molecules in a first support based on at least one physicochemical property of said at least one first member of the binding pair, (ii) transferring the fractionated molecules from said first support to a second support, wherein said second support is selected from the group consisting of a membrane, a silicone support, a glass support, a quartz support, a polyimide support, an acrylate support, a polymethylmethacrylate support, a ceramic support, a nitrocellulose support, a metal support, an amorphous silicon carbide support, and a polystyrene support that is uniformly coated by a set of microarrays, wherein each microarray of the set of microarrays comprises a plurality of second members of the binding pair with different affinities for the at least one first member of the binding pair and wherein said second members of the binding pair are immobilized in said second support, wherein said transferring of fractionated molecules is carried out under conditions suitable for maintaining in the second support a two-dimensional organization of the molecules fractionated in the first support and for allowing interaction between molecules of the population of molecules and the at least one second member of the binding pair coinciding spatially in the second support with said molecules, and (iii) detecting presence of molecules of the population of molecules in association with at least one second member of the binding pair, wherein the presence of molecules of the population of molecules associated with at least one second member of said binding pair in at least one microarray of the set of microarrays is indicative of the presence in the population of molecules of a first member of the binding pair with the capacity to bind specifically to said second member of the binding pair having physicochemical properties corresponding to a position in the first support coinciding spatially with one or more microarrays in which said first member of the binding pair is detected, and further wherein a first member of the at least one first member of the binding pair, the presence of which is to be determined in the population of molecules, is a glycoprotein, and the second member of the binding pair with the capacity to bind specifically to said first member of the binding pair is a lectin.