Patent ID: 9334328
Filing Date: 2016-05-10
CPC Classification: A61K,C07H,C07K,C12N,C12P,G01N

Claim Text:
1. A method of synthesizing a ribonucleic acid (RNA), comprising the steps of: a) providing a complementary deoxyribonucleic acid (cDNA) that encodes a protein of interest; b) selecting a nucleotide that is known to disrupt a binding of a major groove binding partner with a RNA, wherein the nucleotide has decreased binding affinity to the major groove binding partner selected from the group consisting of toll-like receptor (TLR) 3, TLR7, TLR8, retinoic acid-inducible gene I (RIG-I), melanoma differentiation-associated gene 5 (MDA5) and laboratory of genetics and physiology 2 (LGP2), and wherein the nucleotide comprises a modification on the major groove face of the nucleobase where an atom of the major groove face of the nucleobase is replaced or substituted with an alkyl group; and c) contacting the provided cDNA and the selected nucleotide with an RNA polymerase, under conditions such that the RNA is synthesized.