Patent ID: 9297006
Filing Date: 2016-03-29
CPC Classification: C12N,C12Q

Claim Text:
1. A method for parallel amplification of a collection of single nucleic acid molecules having different sequences comprising: a) providing a vessel comprising the collection of single nucleic acid molecules, b) treating the single nucleic acid molecules in the vessel by attaching a known, common sequence at the 5′ and 3′ ends of each nucleic acid molecule of the collection, thereby generating a collection of treated nucleic acid molecules wherein each of the collection of treated nucleic acid molecules contains a different sequence and the known, common sequence at its 5′ and 3′ ends, c) separating the collection of treated nucleic molecules such that individual molecules from the collection of treated nucleic acid molecules are separated from each other, and d) performing a single amplification reaction in the presence of forward colony primers and reverse colony primers complementary to and/or identical to the known common sequence, wherein at least one of the colony primers is immobilized on a solid support, thereby simultaneously amplifying the different sequence of each of the treated nucleic acid molecules separated in step c) under conditions such that a plurality of nucleic acid colonies is formed on the solid support and the collection of single nucleic acid molecules having different sequences is amplified.