Patent ID: 9340826
Filing Date: 2016-05-17
CPC Classification: C12N,C12Q

Claim Text:
1. A method of preparing nucleic acid molecules, comprising (a) providing nucleic acid fragments constituting at least a portion of the complete sequence of a target nucleic acid, wherein step (a) comprises (a-1) providing a pool of oligonucleotides, each containing enzymatic cleavage sites and generic flanking sequences at at least one end, (a-2) cleaving the enzymatic cleavage sites to provide a pool of mixtures comprising the oligonucleotides, wherein at least one cleaved oligonucleotide comprises the generic flanking sequence at at least one end, and (a-3) randomly synthesizing nucleic acid fragments by assembling the oligonucleotides in the mixture using the generic flanking sequences: (b) tagging the nucleic acid fragments with barcode sequences; (c) validating the sequences of the nucleic acid fragments tagged with the barcode sequences, wherein the sequences of the tagged nucleic acid fragments are validated by parallel sequencing; (d) recovering desired nucleic acid fragments among the sequence-validated nucleic acid fragments using the barcode sequences; (e) forming long nucleic acid molecules by assembling the recovered nucleic acid fragments.