Patent ID: 9528087
Filing Date: 2016-12-27
CPC Classification: A61K,A61P,C12N

Claim Text:
1. A method of obtaining a neural multipotent or unipotent cell, comprising: i) providing a cell of a first type which is not a neural multipotent or unipotent cell; ii) introducing into the cell of a first type an agent capable of remodeling the chromatin and/or DNA of the cell, wherein the agent capable of remodeling the chromatin and/or DNA is a histone acetylator, an inhibitor of histone deacetylation, a DNA demethylator, and/or a chemical inhibitor of DNA methylation; iii) introducing into the cell of a first type a reprogramming polypeptide and/or a polynucleotide encoding said reprogramming polypeptide, wherein the reprogramming polypeptide comprises Musashi1 (Msi1) and/or Neurogenin 2 (Ngn2); and iv) placing or maintaining the cell in a neural cell culture medium and maintaining intracellular levels of the reprogramming polypeptide or the polynucleotide encoding the reprogramming polypeptide for a sufficient period of time to allow a neural multipotent or unipotent cell to be obtained.