Patent ID: 9273364
Filing Date: 2016-03-01
CPC Classification: C12N,C12Q

Claim Text:
1. A method for detecting an alternative splicing of a specific gene in a mammalian multicellular organism, the method comprising: (a) introducing into the multicellular organism a DNA construct such that at least two different reporter genes are inserted into the specific gene which undergoes the alternative splicing, wherein the at least two different reporter genes are connected in tandem at the 3′ side of the specific gene in a different reading frame in the DNA construct, and wherein the transcripts of the at least two different reporter genes are each connected in frame to a different exon which is included in at least two different mature mRNAs generated by alternative splicing of the specific gene, wherein the transcripts of the at least two different reporter genes are both present on each of the at least two different mature mRNAs and wherein each reporter gene is translated only when the exon to which it is connected is present on the mature mRNA; and (b) detecting the alternative splicing of the specific gene in the multicellular organism by the expression of the reporter genes, wherein the mammalian multicellular organism is a rodent, and wherein the DNA construct comprises an N-terminal tag.