Patent ID: 9291535
Filing Date: 2016-03-22
CPC Classification: B01J,B01L,G01N

Claim Text:
1. An apparatus for isolating metabolites of vitamin D or small molecules from a non-acidized serum or plasma sample comprising proteins, metabolites of vitamin D, small molecules, phospholipids and interfering compounds in an aqueous media, said apparatus comprising: at least one well having a generally tubular side wall defining an interior well space having an upper inlet and a lower outlet; a first protein crash frit disposed in the interior well space between the upper inlet and the lower outlet defining an introduction zone above the first protein crash frit and between the upper inlet and the first protein crash frit; a first sorbent zone comprising a cation exchanged silica sorbent disposed adjacent to and below the first protein crash frit; a second sorbent zone disposed below said first adsorbent zone comprising an acidized alumina sorbent; a second protein crash frit disposed in the interior well space between the first sorbent zone and the second sorbent zone; and, a lower membrane zone being a hydrophobic protein crash plate disposed adjacent to and below the second sorbent zone, wherein the hydrophobic protein crash plate is a multi-layer protein crash plate constructed of at least three layers selected from the group consisting of polypropylene, polyethylene (PE), polyvinylidene difluoride (PVDF), polytetrafluoroethylene (PTFE), glass, and combinations thereof.