Patent ID: 9499861
Filing Date: 2016-11-22
CPC Classification: C12Q

Claim Text:
1. A method for assaying at least one target nucleic acid molecule, comprising: (a) providing a reaction mixture comprising a nucleic acid sample containing at least one template nucleic acid molecule, a primer pair and a polymerase, wherein said primer pair has sequence complementarity with said template nucleic acid molecule, and wherein said primer pair comprises a limiting primer and an excess primer; (b) subjecting said reaction mixture to a nucleic acid amplification reaction under conditions that are sufficient to yield said at least one target nucleic acid molecule as an amplification product of said template nucleic acid molecule and said limiting primer, which at least one target nucleic acid molecule comprises said limiting primer; (c) bringing said reaction mixture in contact with a sensor array having (i) a substrate comprising a plurality of probes immobilized to a surface of said substrate at different individually addressable locations, wherein said probes have sequence complementarity with said limiting primer and are capable of capturing said limiting primer, and (ii) an array of detectors configured to detect at least one signal from said addressable locations, wherein said at least one signal is indicative of said limiting primer binding with an individual probe of said plurality of probes; (d) using said array of detectors to detect said at least one signal from one or more said addressable locations at multiple time points during said nucleic acid amplification reaction; and (e) detecting said target nucleic acid molecule based on said at least one signal indicative of said limiting primer binding with said individual probe of said plurality of probes.