Patent ID: 9476093
Filing Date: 2016-10-25
CPC Classification: C07H,C12N,C12Q,C12Y

Claim Text:
1. A method for amplifying a target nucleic acid by an activation by polyphosphorolysis (APP) reaction comprising the steps of: (a) annealing to a template strand of the target nucleic acid a complementary activatable oligonucleotide P* having a non-extendable nucleotide at its 3′ terminus and having no mismatched nucleotides at or near its 3′ terminus, so that the terminal nucleotide is hybridized to the template strand when the oligonucleotide P* is annealed, (b) polyphosphorolyzing the resulting duplex with: (c) polymerizing a nucleic acid by extending the activated oligonucleotide P* on the template strand in presence of four nucleoside triphosphates and a nucleic acid polymerase to synthesize the desired nucleic acid strand; and (d) repeating the steps (a)-(c) as necessary to amplify the target nucleic acid.