Patent ID: 9238795
Filing Date: 2016-01-19
CPC Classification: C12N

Claim Text:
1. A method for producing a population of embryonic-lineage specific smooth muscle cells (SMCs) comprising; (i) providing a population of human pluripotent stem cells, (ii) culturing the population of human pluripotent stem cells in an early mesoderm induction medium to produce a population of early mesoderm cells, wherein the early mesoderm induction medium is a chemically defined medium (CDM) which has fibroblast growth factor activity, stimulates SMAD1, SMAD5 and SMAD9 mediated signalling pathways and inhibits phosphatidylinositol 3-kinase (PI3K) activity, (iii) either; a) culturing the population of early mesoderm cells in a lateral mesoderm induction medium to produce a population of lateral mesodermal progenitor cells, wherein the lateral mesoderm induction medium is a chemically defined medium (CDM) which has fibroblast growth factor activity and stimulates SMAD1, SMAD5 and SMAD9 mediated signalling pathways; or b) culturing the population of early mesoderm cells in a paraxial mesoderm induction medium to produce a population of paraxial mesodermal progenitor cells, wherein the paraxial mesoderm induction medium is a chemically defined medium (CDM) which has fibroblast growth factor activity and inhibits phosphatidylinositol 3-kinase (PI3K) activity, and; (iv) culturing the population of lateral or paraxial mesodermal progenitor cells in an SMC induction medium to produce a population of lateral or paraxial mesodermal SMCs of the embryonic lineage.