Patent ID: 9481902
Filing Date: 2016-11-01
CPC Classification: C07K,C12N,C12P,C12Y

Claim Text:
1. A composition comprising an aqueous buffer permitting glycosyltransferase enzymatic activity, the composition further comprising: (a) a glycosylated target molecule, the target molecule being selected from a glycoprotein and a glycolipid, the target molecule comprising a plurality of antennae, at least two of the antennae each having as terminal structure a β-D-galactosyl-1,4-N-acetyl-β-D-glucosamine moiety with a hydroxyl group at the C6 position in the galactosyl residue; (b) N-terminally truncated human β-galactoside-α-2,6-sialyltransferase I of SEQ ID NO:2 (Δ89 hST6Gal-I); (c) N-terminally truncated human β-galactoside-α-2,6-sialyltransferase I of SEQ ID NO:3 (Δ108 hST6Gal-I); and (d) cytidine-5′-monophospho-N-acetylneuraminic acid as donor compound for a sialyltransferase-catalyzed reaction.