Patent ID: 9476878
Filing Date: 2016-10-25
CPC Classification: A61P,C12N,G01N

Claim Text:
1. A method for the detection of polypeptide-specific immune cells, comprising the following steps: a) incubating polypeptides in a solution comprising cyanate ions and in the absence of urea, wherein the cyanate ions are present at a concentration in the range from about 1 to about 1000 mmol/l at temperatures in the range from −20° C. to 100° C. and at an incubation time of about several minutes to several days, b) incubating APC-containing cell cultures or body fluids with the polypeptides of step (a) in the presence of urea, c) incubating the APC-containing cell cultures or body fluids obtained according to step b) with immune cells or immune-cell-containing body fluids, and d) simultaneously and/or specifically detecting and/or quantifying various subtypes of immune cells which are specific against the polypeptides from step a).