Patent ID: 9528154
Filing Date: 2016-12-27
CPC Classification: C12Q

Claim Text:
1. A method for determining the differentiation state of a pluripotent stem cell, wherein said stem cell is one of a population of stem cells, said method comprising: (a) contacting said stem cell with a heterogeneous Cot-1 nucleic acid probe under conditions for hybridization of the heterogeneous Cot-1 nucleic acid probe to a ribonucleic acid molecule in the nucleus of said stem cell; and (b) detecting the hybridization of said heterogeneous Cot-1 nucleic acid probe to the ribonucleic acid molecule in the stem cell, wherein the hybridization of the heterogeneous Cot-1 nucleic acid probe to the ribonucleic acid molecule in the stem cell is detected as a teloRNA mark in situ using fluorescent in situ hybridization (FISH), wherein the presence of two teloRNA marks, one on each sex chromosome, identifies said stem cell as a stem cell that is undifferentiated and the presence of only one teloRNA mark on an inactive X chromosome in a female stem cell or a Y chromosome in a male stem cell identifies said stem cell as a stem cell that is differentiated.