Patent ID: 9422553
Filing Date: 2016-08-23
CPC Classification: C12N

Claim Text:
1. A method for inactivating a target polynucleotide in a cell comprising: introducing into a cell a psiRNA comprising at least 23 nucleotides, wherein the psiRNA comprises a psiRNA-tag and a guide sequence, wherein the psiRNA-tag is the first 5 to 10 nucleotides of the psiRNA and comprises a nucleotide sequence chosen from nucleotides of a repeat from a CRISPR locus that are immediately upstream of a spacer present in a microbe comprising the CRISPR locus, wherein the guide sequence is located immediately downstream of the psiRNA-tag and comprises the remaining nucleotides of the psiRNA, and wherein the guide sequence is complementary to, and hybridizes to, a target polynucleotide which is cleaved.