Patent ID: 9441247
Filing Date: 2016-09-13
CPC Classification: A61K,A61P,C07K,C12N

Claim Text:
1. A method for preparing propagation-defective TC-83 derived alphaviral replicon particles (ARPs), said method comprising: (a) introducing an alphaviral replicon nucleic acid into a host cell, said replicon nucleic acid comprising at least a virus packaging signal and at least one heterologous coding or functional sequence expressible in said alphaviral replicon nucleic acid, said replicon nucleic acid lacking sequences encoding an alphavirus structural protein, wherein said host cell comprises at least one helper function encoding the TC-83 structural proteins comprising the TC-83 capsid protein and the TC-83 glycoproteins, to produce a modified host cell; and (b) culturing said modified host cell under conditions allowing expression of the at least one helper function, allowing replication of said TC-83-derived alphaviral replicon nucleic acid and packaging of said alphaviral replicon nucleic acid to form ARPs.