Patent ID: 9243287
Filing Date: 2016-01-26
CPC Classification: C12Q

Claim Text:
1. A method for determining event zygosity of a cotton plant comprising a 3006-210-23 event, said 3006-210-23 event comprising a transgene construct comprising a cry1Ac gene, said method comprising: obtaining a sample of genomic DNA from said cotton plant, contacting said sample with wherein said first flanking primer and said second flanking primer form a wild-type amplicon when subjected to PCR conditions, wherein said transgene primer forms a transgene amplicon with said first flanking primer or said second flanking primer when subjected to PCR conditions, further contacting said sample with subjecting said sample to fluorescence-based endpoint TaqMan PCR conditions, quantitating said florescent event probe that hybridized to said event amplicon, quantitating said florescent wild-type probe that hybridized to said wild-type amplicon, comparing amounts of hybridized florescent event probe to hybridized florescent wild-type probe; and determining zygosity of said cotton tissue by comparing florescence ratios of hybridized fluorescent event probe and hybridized fluorescent wild-type probe.