Patent ID: 9465029
Filing Date: 2016-10-11
CPC Classification: C12Q,G01N

Claim Text:
1. A colorimetric method of determining the percentage of inhibition of Lp-PLA2 activity, the method comprising: preparing a solution comprising a substrate for Lp-PLA2 having a colorimetric detectable moiety, which substrate is 1-myristoyl-2-(4-nitrophenylsuccinyl)phosphatidylcholine; contacting a first blood sample that is taken from a human subject at a first time with the solution; colorimetrically detecting Lp-PLA2 activity from the first blood sample wherein the first blood sample is diluted less than 33 times; contacting a second blood sample that is taken from the subject at a second time one hour or more after the first time with the solution, wherein the second blood sample is taken after the subject has been administered a drug that inhibits Lp-PLA2; colorimetrically detecting Lp-PLA2 activity from the second blood sample wherein the second blood sample is diluted less than 33 times; and detecting greater than 30% inhibition of Lp-PLA2 activity between the first and second blood samples by comparing the activity of the Lp-PLA2 between the first and second blood samples, indicating that the subject has taken a drug that inhibits Lp-PLA2.