Patent ID: 9296999
Filing Date: 2016-03-29
CPC Classification: C12N,C12P,C12Q,C12Y,Y02P

Claim Text:
1. A composition comprising a φ29-type (phi29-type) recombinant DNA polymerase, which recombinant polymerase comprises an amino acid sequence that is at least 80% identical to SEQ ID NO:1, and which recombinant polymerase comprises one or more mutation selected from the group consisting of an amino acid substitution at position E20, an amino acid substitution at position R308, a Q99W substitution, a K131S substitution, an R261Q substitution, a C290F substitution, an R306Q substitution, a C455A substitution, an E466K substitution, an E466R substitution, and an E508Q substitution, wherein identification of positions is relative to SEQ ID NO:1, and wherein said polymerase exhibits polymerase activity.