Patent ID: 9273298
Filing Date: 2016-03-01
CPC Classification: C12N,C12P,C12Y

Claim Text:
1. A recombinant microbial host cell comprising a nucleic acid encoding a polypeptide having isoprene synthase activity and providing improved growth activity when the polypeptide is expressed in a host cell, wherein the polypeptide has at least 90% sequence identity to SEQ ID NO:1 and comprises a substitution at a residue corresponding to K134, K138, L143, I156, E159, F163, S166, H167, E170, K414, or Q421 of SEQ ID NO:1, wherein the substitution is selected from the group consisting of K134P, K138C, L143F, L143V, I156G, E159G, E159Q, F163C, F163E, F163Q, F163V, F163Y, S166C, S166D, S166G, S166P, S166V, H167M, E170G, E170H, E170K, E170N, E170R, E170S, E170W, K414F, K414G, K414N, K414P, and Q421R, and wherein a host cell expressing the polypeptide has at least 50% more growth compared to the growth of a host cell expressing a parent polypeptide which does not comprise said substitution at a residue corresponding to SEQ ID NO:1 under similar growth conditions.