Patent ID: 9447459
Filing Date: 2016-09-20
CPC Classification: C12N,C12Q,C40B,G16B

Claim Text:
1. A method for identifying one or more polymorphisms, comprising: (a) ligating sequencing adapters carrying a 3′-T overhang to at least a first library of amplified fragments comprising sequences of a first nucleic acid sample and a second library of amplified fragments comprising sequences of a second nucleic acid sample to obtain first and second libraries of sequencing adapter-ligated fragments; (b) combining the first and second libraries of sequencing adapter-ligated fragments; (c) sequencing at least a portion of the combined libraries to obtain sequences; (d) aligning the sequences obtained in step (c) to obtain an alignment; and (e) determining one or more polymorphisms between the first and second nucleic acid samples in the alignment of step (d).