Patent ID: 9434992
Filing Date: 2016-09-06
CPC Classification: A01K,A61K,A61P,C07K,C12Q

Claim Text:
1. A method for diagnosing amyotrophic lateral sclerosis in a human subject, comprising: (a) obtaining a sample from a human subject suspected of amyotrophic lateral sclerosis, (b) isolating nucleic acids from the sample, (c) amplifying DNA fragments from exon 12 of the OPTN (Optineurin) gene by polymerase chain reaction using primers having the sequence of SEQ ID NO: 5 and 6, (d) determining whether the base in exon 12 of the OPTN at position 13207995 of chromosome 10 is mutated, and (e) diagnosing that the subject has amyotrophic lateral sclerosis if the base is mutated.