Patent ID: 9395367
Filing Date: 2016-07-19
CPC Classification: G01N

Claim Text:
1. A method comprising: collecting a sample suspected of containing at least one B cell comprising at least one B Cell Receptor (“BCR”); stimulating the at least one B cell with a first aliquot of an antigen; washing away any excess antigen of the first aliquot; labeling the at least one BCR with at least one receptor probe comprising a fluorescent molecule bound to a ligand, wherein the ligand is bound to the at least one BCR; imaging the sample to obtain at least one first image; re-stimulating the at least one B cell with a second aliquot of the antigen; re-imaging the sample to obtain at least one second image; comparing the at least one first image and the at least one second image to determine that the at least one B cell was re-stimulated in response to the second aliquot of the antigen; isolating the at least one B cell that has been re-stimulated; sequencing the at least one BCR from said cell; and generating a monoclonal antibody based on the sequence of said the at least one BCR.