Patent ID: 9290739
Filing Date: 2016-03-22
CPC Classification: A61K,A61P,C12N

Claim Text:
1. A method for preparing equine amniotic fluid-derived multipotent stem cells, comprising Step 1 of isolating mononuclear cells from equine amniotic Step 2 of culturing the mononuclear cells in basal culture media for a period time, wherein the cultured mononuclear cells are adherent; and wherein the basal culture media comprises an endothelial cell growth media containing vascular endothelial growth factor (VEGF), long R Step 3 of culturing the adherent cells in Dulbecco's Modified Eagle's Medium (DMEM) after culturing in the basal media; and Step 4 of recovering the stem cells from the culture, wherein the recovered multipotent stem cells: (a) are negative for the human markers CD19, CD20, CD28, CD31, CD34, CD38, CD41a, CD62L, CD62P, and CD200, and (b) are positive the human markers CD44, CD90, and CD105; (c) have the ability to differentiate into, mesoderm derived cells; (d) have the ability to maintain an undifferentiated state for 14 passages or more; and (e) are more homogeneous than those before culture.