Patent ID: 9274101
Filing Date: 2016-03-01
CPC Classification: C12N,C12Q,G01N

Claim Text:
1. A method for phenotyping animal cells, comprising the steps of: a) providing: b) introducing a first portion of said suspension medium into said positive control well and said negative control well and incubating said positive control well and said negative control well; c) introducing a first portion of said redox indicator into said positive control well and said negative control well, wherein an adaptation period length is determined by greater measurable color developed in said positive control well as compared to measurable color developed in said negative control well; d) introducing a second portion of said suspension medium into said plurality of testing wells; e) pre-incubating said second portion of said suspension medium in said plurality of testing wells for said adaptation period length; f) introducing a second portion of said redox indicator into said plurality of testing wells; g) incubating said second portion of said suspension medium such that said redox indicator is reduced in at least one testing substrate well to produce a color development; h) measuring said color development in said plurality of testing wells to create a metabolic profile; and i) analyzing said metabolic profile to determine phenotypes of said at least one animal cell.