Patent ID: 9476033
Filing Date: 2016-10-25
CPC Classification: A61K,A61P,C12N

Claim Text:
1. An antigenomic RNA of Newcastle disease virus, consisting of: NP gene, P gene, M gene, F gene, HN gene and L gene in this order from a 5′ to 3′ direction, a 55 nucleotide 3′ leader, a 113 nucleotide 5′ terminus, and n foreign nucleotide complexes inserted (a) before the NP gene, (b) between the P and M genes, and/or (c) between the HN and L genes, wherein n is 1, 2, 3 or 4; each of the foreign nucleotide complexes comprising a Newcastle disease virus gene start sequence, an open reading frame of a foreign gene and a Newcastle disease virus gene end sequence in this order from the 5′ to 3′ direction, wherein the foreign gene is a gene not found naturally in the Newcastle disease virus; wherein when 2, 3 or 4 foreign nucleotide complexes are inserted together before the NP gene, between the P and M genes, or between the HN and L genes, the foreign nucleotide complexes are sequentially linked directly or indirectly; wherein the antigenomic RNA of Newcastle disease virus has a length of m nucleotides, wherein m is a multiple of 6, wherein three G residues are inserted before the 5′ terminus, and wherein the Newcastle disease virus has an F