Patent ID: 9528153
Filing Date: 2016-12-27
CPC Classification: B82Y,C12Q,G01N,Y10S

Claim Text:
1. A method for sequencing a double strand molecule, the method comprising: driving the double strand molecule into a first Y-channel and a second Y-channel of a membrane by a first voltage pulse, the first Y-channel includes a first stem and first branches, the first branches being connected to the first stem at a first junction; wherein the second Y-channel includes a second stem and second branches formed in the membrane, the second branches being connected to the second stem at a second junction, wherein at least one of the second branches of the second Y-channel is connected to at least one of the first branches of the first Y-channel such that one or more of the first branches feeds into the second Y-channel; slowing the double strand molecule at the first junction of the first Y-channel based on the first voltage pulse being weaker than a force required to break a base pair of the double strand molecule; splitting the double strand molecule into a first single strand and a second single strand by driving the double strand molecule into the first junction of the first Y-channel at a second voltage pulse; and sequencing the first single strand in one of the branches and sequencing the second single strand in another one of the branches.