Patent ID: 9354238
Filing Date: 2016-05-31
CPC Classification: G01N,Y10T

Claim Text:
1. A method of screening for antibodies that bind native human leukocyte antigens, comprising the steps of: a. contacting a sample with a composition comprising 1) a solid substrate having linked thereto purified human leukocyte antigens (HLA) wherein at least 90% of the purified HLA linked to the solid substrate are native HLA and at most 10% of the purified HLA linked to the solid substrate are denatured HLA and 2) a neutralized enzyme selected from the group consisting of a trypsin-like clan protease, trypsin, chymotrypsin and elastase, and b. detecting binding of an antibody to the solid substrate, wherein binding of an antibody to the solid substrate is indicative of a sample comprising antibodies specific for said native human leukocyte antigens.