Patent ID: 9267168
Filing Date: 2016-02-23
CPC Classification: C12N,C12Q

Claim Text:
1. A method for isolating circular template nucleic acids containing one or more target sequences from a mixture of nucleic acids, said method comprising: (a) providing a population of nucleic acid constructs, said constructs comprising circular template nucleic acids, wherein a plurality of said circular template nucleic acids comprises said one or more target sequences; (b) amplifying said population of nucleic acid constructs with a strand displacing polymerase to produce amplified template nucleic acids, wherein said amplified template nucleic acids each comprise one of said circular template nucleic acids hybridized to a linear nascent strand comprising at least one copy of a sequence complementary to said one or more target sequences; (c) associating one or more affinity handles with said amplified template nucleic acids, wherein said one or more affinity handles comprises an affinity oligonucleotide moiety and a retrieval moiety, wherein said affinity oligonucleotide moiety hybridizes to said at least one copy of a sequence complementary to said one or more target sequences; (d) separating amplified template nucleic acids associated with said affinity handles from amplified template nucleic acids not associated with said affinity handles using said retrieval moieties; (e) after said separating, treating said amplified template nucleic acids such that said circular template nucleic acids are separated from said linear nascent strands; and (f) isolating said circular template nucleic acids containing one or more target sequences from the mixture of nucleic acids.