Patent ID: 9382516
Filing Date: 2016-07-05
CPC Classification: A61P,C12N

Claim Text:
1. A method of preparing a self-propagating dopaminergic neuron cell specific to a human patient, the method comprising: (a) obtaining a human cancer cell; (b) treating the human cancer cell with a DNA interchelating agent to obtain a DNA interchelating agent treated human cancer cell, (c) treating the DNA interchelating agent treated human cancer cell with an agglutinin to obtain an agglutinin-treated human cancer cell; (d) fusing the agglutinin-treated human cancer cell from step (c) with a human dopaminergic neuron cell to obtain a self-propagating human dopaminergic neuron cytoplasmic donor cell; (e) treating the self-propagating human dopaminergic neuron cytoplasmic donor cell from step (d) with a DNA interchelating agent to obtain a DNA interchelating agent treated human dopaminergic neuron cytoplasmic donor cell; (f) treating the DNA interchelating agent treated human dopaminergic neuron cytoplasmic donor cell from step (e) with an agglutinin to obtain an agglutinin-treated human dopaminergic neuron cytoplasmic donor cell; and (g) fusing the agglutinin-treated human dopaminergic neuron cytoplasmic donor cell from step (f) with a histone deacetylase inhibitor treated somatic cell from a human patient whereby a self-propagating dopaminergic neuron cell specific to the human patient is obtained.