Patent ID: 9528981
Filing Date: 2016-12-27
CPC Classification: A61P,G01N

Claim Text:
1. Method for the isolation of immune system cells having cytolytic activity from a population of immune system cells previously collected from a human being, comprising the steps of: a) interacting the population of immune system cells with target cells by inducing the contact of a single cell from the population of immune system cells with a target cell, wherein the immune system cells having cytolytic activity are capable of inducing lysis in the target cells; b) identifying which target cells have undergone lysis; and c) selecting immune system cells having cytolytic activity by displacing on a single cell basis only those immune system cells that have induced said lysis of the target cells within 20 minutes of interacting the population of immune system cells with target cells; wherein said steps a), b), and c) are carried out by displacing single cells using a miniaturized device based on a dielectrophoresis-based array which allows real-time monitoring and manipulation of single immune system cells having cytolytic activity without altering the cytolytic activity of the immune system cells having cytolytic activity.