Patent ID: 9234887
Filing Date: 2016-01-12
CPC Classification: G01N,Y10T

Claim Text:
1. Method for the determining of content of lactic acid or lactate in biological fluids including the following steps: providing a biological fluid, providing a selected amount of Fe(III), or other redox couple oxidized metal/reduced metal oxidant reagent able to react selectively with lactic acid or lactate, mixing of the biological fluid and the oxidant reagent; irradiating of the above-mentioned mixing of the biological fluid and the oxidant reagent with UV light in the wavelength range of 280-400 nm; determining the amount of lactic acid contained in the biological fluid by determining of the reduced species of the oxidant reagent, wherein the oxidant reagent includes a complexing agent of Fe(II) or of the above-mentioned reduced metal species, and the determination of the amount of lactic acid contained in the biological fluid is performed by the determination of the complexed reduced species of the oxidant reagent by spectrophotometry of the above-mentioned complex, characterized in that the complexing agent is 1,10-phenantroline, and the photometric detection of the absorbance being performed at about 512 nm wavelength.