Patent ID: 9441260
Filing Date: 2016-09-13
CPC Classification: C12M,C12Q,G01N

Claim Text:
1. A method for in situ real-time non-invasive monitoring of contaminations in a sealed platelet bag, said method including the steps of: (a) providing a sealed platelet bag comprising: (b) providing a detection apparatus comprising: (c) determining the concentration of carbon dioxide in the dead space of said sealed platelet bag or in said reservoir in fluid communication with said dead space by: wherein an increase of the concentration of carbon dioxide in said dead space or in said reservoir in fluid communication with said dead space relative to a reference concentration is indicative of a contamination, and wherein determining the concentration of carbon dioxide in step (c)(iii) is made via a nonlinear minimization model S(x, λi) as provided by function s(x) below: where ε is a noise level at the detector, and S(x, λi) is provided by the following equation: where b is a constant, ƒ(λ−λi) is the laser spectral distribution function around the central wavelength λi, αλ, is the absorption coefficient, x=cl wherein c is the gas concentration inside the container, l is the path length inside the container, c 0 is the concentration of the probed gas outside the container and l 0 is the path length outside the container between the infrared source and the detector.