Patent ID: 9395283
Filing Date: 2016-07-19
CPC Classification: C12Q,G01N

Claim Text:
1. A method of making an in vitro solid composition comprising a homogenous mixture of cells evenly distributed throughout said composition for use as an experimental positive control, the steps comprising, a. pre-treating cells comprising the steps of, fixing the cells in paraformaldehyde or formaldehyde and phosphate buffered saline (PBS), then centrifuging and rinsing the cells; b. passing the pre-treated cells vertically through holes in a multi-chambered-tiered apparatus and mixing the pre-treated cells with a fluid simultaneously to create a homogenous cell mixture of immobilized cells, wherein the multi-chambered-tiered apparatus comprises, c. injecting the homogenous cell mixture into a first mold, letting the mixture set to immobilize the cell mixture, and removing a solid composition from the first mold; d. processing the solid composition with paraffin, removing the solid composition from the paraffin, and embedding the solid composition into a second mold to produce formalin fixed paraffin embedded (FFPE) cells; and, e. removing the solid composition from the second mold, wherein the solid composition comprises a homogenous mixture of formalin fixed paraffin embedded (FFPE) cells with a uniform density of cells throughout the solid composition.