Patent ID: 9458491
Filing Date: 2016-10-04
CPC Classification: C12N,C12Q,C12R,G01N

Claim Text:
1. A method for measuring a concentration of an analyte in a test solution wherein the analyte is i) mevalonic acid and/or 3-hydroxymethylglutaryl coenzyme A, or ii) coenzyme A, the method comprising the following steps (p) and (q): (p) a step of allowing an enzyme that catalyzes a reaction represented by Reaction Formula 1′: and an enzyme that catalyzes a reaction represented by Reaction Formula 2′: to act on the test solution containing the analyte (i) in the presence of a hydrogen acceptor X, a hydrogen donor Y, and coenzyme A, where the analyte is mevalonic acid and/or 3-hydroxymethylglutaryl coenzyme A, to produce both a reduced hydrogen acceptor X and an oxidized hydrogen donor Y, wherein the hydrogen donor Y and the reduced hydrogen acceptor X are not the same or (ii) in the presence of a hydrogen acceptor X, a hydrogen donor Y, and mevalonic acid, where the analyte is coenzyme A, to produce both a reduced hydrogen acceptor X and an oxidized hydrogen donor Y, wherein the hydrogen donor Y and the reduced hydrogen acceptor X are not the same; and (q) a step of measuring an amount of: a reduced hydrogen acceptor X that is produced; or an oxidized hydrogen donor Y that is produced; or a hydrogen acceptor X that is decreased; or a hydrogen donor Y that is decreased.