Patent ID: 9376719
Filing Date: 2016-06-28
CPC Classification: C12Q,G16B

Claim Text:
1. A method for the detection of a mutation in a target sequence in one or more members of a mutagenized population, comprising: (a) providing a plurality of pools of amplification products each comprising the target sequence amplified from genomic DNA of a subset of the one or more members of the mutagenized population, wherein each pool of the amplification products shares a unique pool identifier; (b) determining the nucleotide sequence of the amplification products using high throughput sequencing; and (c) identifying mutations by clustering/aligning the sequences of the amplified products without the use of an enzyme which recognizes and cuts single nucleotide sequence mismatches and without performing heteroduplex analysis, and identifying the one or more members carrying the mutation using the pool identifier.