Patent ID: 9428557
Filing Date: 2016-08-30
CPC Classification: A61K,C07K,C12N

Claim Text:
1. A process for the preparation of biologically active Tat suitable for human use, comprising the steps of: 1) culturing a host organism capable of expressing Tat when induced in a culture volume of at least 15 L, wherein the host organism is 2) culturing said host organism in a culture media that comprises a vegetable peptone and does not comprise components of animal origin; 3) inducing the expression of Tat during the logarithmic growth phase of the host organism; wherein the induction occurs at 0.6 OD of the host culture, 4) purifying Tat from the culture, wherein the yield is at least 15.8 mg of Tat for a culture volume of 15 L; and wherein the Tat produced is considered biologically active if at a concentration of 100 ng/ml it is taken up by at least 49% of a cell culture of monocyte-derived dendritic cells (MDDC).