Patent ID: 9228172
Filing Date: 2016-01-05
CPC Classification: A61K,A61P,C12N

Claim Text:
1. A method of generating and expanding an inducible T regulatory cell (iTreg), said method comprising isolating a non-Treg from peripheral blood, wherein said non-Treg is selected from the group consisting of a CD4 contacting the non-Treg with a combination of agents capable of converting said non-Treg into an iTreg, wherein the combination of agents comprise a tryptophan catabolite, and a demethylating agent selected from the group consisting of 5-aza-2′-deoxycitidine, 5-Azacytidine, and any combination thereof; contacting the iTreg with a mTOR inhibitor separate from the combination of agents, wherein the mTOR inhibitor inhibits non-Treg growth and is selected from the group consisting of tacrolimus, rapamycin , rapamycin derivative, and any combination thereof; and expanding the iTreg under appropriate growth conditions.