Patent ID: 9366631
Filing Date: 2016-06-14
CPC Classification: B01L,C12Q,G01N,Y10T

Claim Text:
1. A system to detect components separated by electrophoresis comprising: (i) an integrated biochip comprising a thermal cycling chamber adapted for multiplexed amplification in one PCR reaction, said thermal cycling chamber in fluid communication with at least one channel on said biochip, wherein each channel comprises a detection position; (ii) primers labeled with 6 or more fluorescent dyes for generating amplified nucleic acid fragments labeled with said 6 or more fluorescent dyes; (iii) no more than one light source positioned for illuminating said plurality of detection positions over a physical space on said biochip; (iv) one or a plurality of first optical elements selected from the group consisting of lenses, pinholes, mirrors and objectives, positioned over said physical space for collecting and directing light emanating from the plurality of detection positions on said substrate; (v) a scanning mirror galvanometer for sequentially illuminating and collecting light from said light source at each one of said plurality of detection positions; and (vi) a wavelength dispersive element selected from the group consisting of prisms, diffraction gratings, transmission gratings, holographic diffraction gratings and spectrographs positioned to disperse the wavelength components of the fluorescence over a physical space according to light wavelength, and to provide at least a portion of the dispersed wavelength components by reflecting a predetermined wavelength of light which essentially corresponds to a fluorescence emission maximum of a fluorescent dye present in at least one of the detection positions to at least six detectors or a multi-element PMT positioned to detect fluorescence from said labeled amplified nucleic acid fragments.