Patent ID: 9383368
Filing Date: 2016-07-05
CPC Classification: B01L,G01N

Claim Text:
1. A method for diagnosing heparin-induced thrombocytopenia (HIT) in a human subject suspected of having HIT, the method comprising: (a) incubating a liquid sample from the subject in a reaction cell adapted to receive the liquid sample to obtain a reaction mixture, the liquid sample comprising at least one of plasma and serum, and the reaction cell comprising particles complexed to platelet factor 4 (PF4) by adsorption of PF4 directly to the surface of the particles, wherein the particle-complexed PF4 reacts specifically with heparin/platelet factor 4 antibodies in the liquid sample forming specific aggregates in the reaction mixture; (b) applying the reaction mixture obtained in step (a) to a filter well of an assay plate, the assay plate comprising: (c) detecting a presence or absence of the unaggregated particles in the observation well, wherein the detection of the absence of the unaggregated particles in the observation well indicates the presence of the heparin/platelet factor 4 antibodies in the subject, thereby diagnosing HIT in the subject.