Patent ID: 9359612
Filing Date: 2016-06-07
CPC Classification: C07K,C12N,C12P

Claim Text:
1. Recombinant host cells that have been modified to improve production of itaconate, said cells having been modified only to contain a nucleic acid that encodes protein wherein said encoded protein consists of the amino acid sequence of an itaconate transporter protein that transports itaconate acids from the cytosol to the extracellular medium; and optionally nucleic acid that encodes protein wherein said encoded protein consists of the amino acid sequence of the enzyme cis aconic acid decarboxylase (CAD), and optionally nucleic acid that encodes protein wherein said encoded protein consists of the amino acid sequence of a protein capable of transporting di/tricarboxylic acids from the mitochondrion, wherein the itaconate transporter protein has the amino acid sequence of SEQ ID NO:3 or an amino acid sequence at least 95% identical thereto or SEQ ID NO:6, wherein the host cells are heterologous to said nucleic acid.