Patent ID: 9487755
Filing Date: 2016-11-08
CPC Classification: C12N

Claim Text:
1. A cell culture medium for clinical growth of adipose stromal cells from a species to be cultivated for clinical and therapeutic applications, said medium comprising: (a) a basal medium suitable for mammalian cell culture and human albumin for clinical growth of human adipose stromal cells for human clinical and therapeutic applications from the species to be cultivated; (b) at least one of (i) growth promoting amounts of insulin from the species to be cultivated, (ii) transferrin from the species to be cultivated, (iii) recombinant epidermal growth factor from the species to be cultivated at a concentration of about 10.0 ng/ml, (iv) recombinant platelet-derived growth factor-BB from the species to be cultivated at a concentration of about 5.0 ng/ml, and (v) human recombinant basic fibroblast growth factor; (c) L-ascorbate-2-phosphate solution in a concentration of 100 μM; (d) 2-mercaptoethanol in a concentration of 100 μM; (e) dexamethasone solution in a concentration of 1 nM; and (f) phenol red.