Patent ID: 9506105
Filing Date: 2016-11-29
CPC Classification: B01J,B01L,C07H,C12Q,C40B,Y10S,Y10T

Claim Text:
1. A method for amplifying a target nucleic acid present in a sample, the method comprising: introducing, via an entrance opening of a planar fluidic assembly, a sample containing one or more target nucleic acids into a flow channel of the planar fluidic assembly, the flow channel extending from the entrance opening to an exit vent, the flow channel having a substantially uniform cross-section from the entrance opening to the exit vent, wherein a plurality of nucleic acid primers that are complementary to a portion of the one or more target nucleic acids are preloaded and retained at locations within and along the flow channel from the entrance opening to the exit vent; subjecting the sample introduced into the flow channel to a primer-based amplification reaction using the nucleic acid primers, wherein the primer-based amplification reaction produces amplified product of the one or more target nucleic acids; retaining the amplified product at one or more of the locations within the flow channel during the primer-based amplification reaction; and detecting localized accumulation of the retained amplified product, the localized accumulation occurring at the one or more locations.