Patent ID: 9315816
Filing Date: 2016-04-19
CPC Classification: C12N,C12Q

Claim Text:
1. A selection method for a genetic switch and a genetic circuit, the selection method comprising: incubating cells transfected with in a presence or absence of an activating compound that activates the genetic switch, with addition of a first death-determining compound that induces cell death under expression of the controlled gene sequence, and collecting viable cells, and/or in a presence or absence of the activating compound with addition of a second death-determining compound that induces cell death under non-expression of the controlled gene sequence; and collecting viable cells that survive the incubation, wherein the controllable expression vector comprises a first promoter sequence operably linked to and upstream of the controlled gene sequence, and the controlled gene sequence encodes an alkylated DNA repair enzyme; and the genetic circuit expression vector comprises a target sequence of the genetic switch operably linked to the transcription regulatory factor gene sequence and a second promoter sequence different from the first promoter sequence, the transcription regulatory factor gene sequence encoding a transcription factor being operable on the first promoter sequence, and the second promoter sequence being operably linked to the genetic switch expression sequence, incubation being performed (1) when the transcription regulatory factor is a transcription repression factor, in the absence of the activating compound with addition of an alkylating agent, or (2) when the transcription regulatory factor is a transcription activation factor, in the presence of the activating compound with addition of the alkylating agent.