Patent ID: 9371557
Filing Date: 2016-06-21
CPC Classification: C12Q

Claim Text:
1. A method for nucleic acid amplification, comprising: (a) forming a reaction mixture including a continuous liquid phase containing (i) a plurality of separate supports containing a first universal primer that does not include any target-specific sequence, (ii) a second universal primer in solution, (iii) a plurality of different polynucleotide templates containing both a first primer binding sequence and a second primer binding sequence, wherein the first primer binding sequence is complementary or identical to at least a portion of the first universal primer and the second primer binding sequence is complementary or identical to at least a portion of the second universal primer, and (iv) a polymerase; and (b) forming two or more substantially monoclonal populations, each linked to a different support, by clonally amplifying, within the same reaction mixture of step (a) in the continuous liquid phase, at least two of the polynucleotide templates on different separate supports under isothermal amplification conditions using the first and second universal primers by partially denaturing and without compartmentalizing the polynucleotide templates.