Patent ID: 9428789
Filing Date: 2016-08-30
CPC Classification: C12Q,G01N

Claim Text:
1. A method for classifying a kinase inhibitor as a type I or type II kinase inhibitor based on a conformational change that the kinase inhibitor induces in the structure of a kinase labeled with a second harmonic-active label, wherein the label has a net orientation at an interface, the method comprising: a. contacting the kinase with a kinase inhibitor, wherein the kinase specifically interacts with said kinase inhibitor; b. detecting an interaction between the kinase and said kinase inhibitor in solution and in real-time by measuring a first signal or signal change generated by the second harmonic-active label using a surface-selective technique, wherein the first signal or signal change indicates a conformational change in the structure of the kinase that is specific for the kinase inhibitor; and c. classifying the kinase inhibitor as a type I or type II kinase inhibitor by comparing the first signal or signal change of (b) with a second signal or signal change detected by an interaction between the kinase and a known type I or type II inhibitor of the kinase, wherein the second signal or signal change indicates a conformational change in the structure of the kinase that is specific for the known type I or type II inhibitor of the kinase.