Patent ID: 9376705
Filing Date: 2016-06-28
CPC Classification: C12Q,G01N

Claim Text:
1. A method for determining the susceptibility of a microorganisms to chemical agents or antibiotics, comprising: (a) adding a quantity of microbial cells to a liquid nutrient medium; (b) exposing the microbial cells to a chemical or a physical stimulus capable of inducing a stress response, thereby inducing a stress response; (c) pre-incubating the microbial cells in the nutrient medium at a temperature for a time; (d) adding a quantity of a chemical agent or antibiotic to the liquid nutrient medium; (e) incubating the microbial cells at a temperature for a time; (f) removing the microbial cells from the incubated nutrient medium and collecting the supernatant; (g) precipitating the proteins from the supernatant with alcohol; (h) isolating the precipitated protein from the alcohol treated supernatant; (i) adding a quantity of a solvent to the isolated protein to dissolve the isolated protein for mass spectrometric analysis; (j) applying the dissolved protein and a matrix solution to a mass spectrometric sample support and drying the mixture of dissolved protein and matrix solution on the sample support to produce a mass spectrometric sample; (k) acquiring a mass spectrum of the mass spectrometric sample; and (l) comparing the acquired mass spectrum with at least one reference mass spectrum for the same microorganism to determine the susceptibility of the microorganism to the chemical agent or antibiotic.