Patent ID: 9428730
Filing Date: 2016-08-30
CPC Classification: C12N

Claim Text:
1. A neuronal differentiation medium for culturing human adipose derived stem cells consisting of: a neural basal medium, wherein the neural basal medium consists of glycine, L-alanine, L-arginine, L-asparagine, L-cysteine, L-histidine, L-isoleucine, L-leucine, L-lysine, L-methionine, L-phenylalanine, L-proline, L-serine, L-threonine, L-tryptophan, L-tyrosine, L-valine, choline, D-calcium pantothenate, folic acid, niacinamide, pyridoxine hydrochloride, riboflavin, thiamine hydrochloride, vitamin B12, i-inositol, calcium chloride, ferric nitrate, magnesium chloride, potassium chloride, sodium bicarbonate, sodium chloride, sodium phosphate, zinc sulfate, dextrose, 4-(Z-hydroxyethyl)-1-piperazineethanesulfonic acid, sodium pyruvate, epidermal growth factor (EGF) at a concentration about 1 to about 100 ng/mL, b-fibroblast growth factor (b-FGF) at a concentration of about 1 to 100 ng/mL, and N2 supplement at a concentration of about 1x, wherein the medium promotes neurogenesis, and wherein the neuronal differentiation medium is substantially free of serum.