Patent ID: 9491943
Filing Date: 2016-11-15
CPC Classification: A01N,C12N,C12P,C12Y,Y02P

Claim Text:
1. A bioengineering strain for production of microorganism-originated fungicides, wherein the bioengineering strain is obtained by transforming a phzH gene expression plasmid into a strain producing phenazine-1-carboxylic acid, wherein the bioengineering strain produces phenazine-1-carboxamide; the phzH gene expression plasmid is an expression plasmid cloned with a phzH gene fragment; the phzH gene expression plasmid expresses phzH gene in the strain producing phenazine-1-carboxylic acid where the phzH gene is encoded to produce PhzH (glutamine phenazine-1-carboxylic acid amidotransferase), the amino acid sequence of the PhzH is SEQ ID NO:1; the PhzH is a PhzH of Pseudomonas aeruginosa strains PAO1, and the phzH gene segment is a phzH gene segment of Pseudomonas aeruginosa strains PAO1; the nucleotide base sequence of the phzH gene fragment comprises a complete coding region of the phzH gene and a 5′-end noncoding region; the sequence of the 5′-end noncoding region comprises a polynucleotide segment starting from the first nucleotide base to the 683th nucleotide base of SEQ ID NO.2; and the strain producing phenazine-1-carboxylic acid is a growth-promoting antagonistic bacterium M18 or an engineering strain M18G.