Patent ID: 9309542
Filing Date: 2016-04-12
CPC Classification: C12N,C12P,Y02E

Claim Text:
1. A recombinant Caldicellulosiruptor microbe genetically modified to produce a greater amount of ethanol than a comparable wild type control, wherein the Caldicellulosiruptor microbe: comprises a knock-out of the coding region of a lactate dehydrogenase and produces lactate in an amount less than a comparable wild type control; comprises at least one heterologous polynucleotide that encodes an enzyme in a metabolic pathway that converts acetyl-CoA to ethanol, wherein the enzyme is an acetaldehyde dehydrogenase that can convert acetyl-CoA to acetaldehyde, an aldehyde dehydrogenase that can convert acetyl-CoA to acetaldehyde, or a combination thereof; and wherein the