Patent ID: 9441028
Filing Date: 2016-09-13
CPC Classification: B01D,C07K

Claim Text:
1. A method for the chromatographic separation of a polypeptide of interest from a weaker bound component in a fluid mixture, comprising: providing an open loop simulated moving bed counter current purification system comprising a plurality of sections in fluid connection, wherein the sections comprise at least one solid phase and a first desorbent stream; introducing the fluid mixture as a feed stream into the counter current purification system to allow the fluid mixture to contact the at least one solid phase in a counter current mode, and wherein the concentration of the weaker bound component in the fluid mixture is lower than the concentration of the polypeptide of interest; controlling the isotherm resulting from the introducing step by adjusting the concentration of at least one modifier in the feed stream so that the initial slope of the isotherm varies up to 10% from the slope of the operating line when a plot is made of dimensionless flow (m separating the polypeptide of interest from the weaker bound component; wherein the chromatographic separation does not include size exclusion chromatography.