Patent ID: 9499866
Filing Date: 2016-11-22
CPC Classification: C12Q

Claim Text:
1. A method of detecting a single nucleotide polymorphism in a KRAS gene, comprising: (a) hybridizing a set of probes to a DNA sample from a human subject, the set of probes comprising: a first probe comprising a nucleic acid sequence set forth as SEQ ID NO: 91, wherein the nucleic acid sequence of the first probe comprises a nucleation sequence bound to templated metal nanoclusters that are fluorescent, and a second probe comprising a nucleic acid sequence set forth as SEQ ID NO: 92, wherein the nucleic acid sequence of the second probe comprises an enhancer sequence that enhances the fluorescence of the templated metal nanoclusters; and wherein: the first and second probes hybridized to the DNA sample form a three-way junction wherein the templated metal nanoclusters of the first probe are proximal to the enhancer sequence of the second probe; the polymorphism is positioned at a branch point of the three-way junction; the presence of the polymorphism causes a shift in alignment of the templated metal nanoclusters relative to the enhancer sequence compared to a wild-type control, resulting in a detectable change in a fluorescence emission wavelength of the metal nanoclusters; and wherein the probes do not hybridize to non-target DNA; (b) measuring the fluorescence emission wavelength of the probes hybridized to the DNA sample in response to ultraviolet light, visible light, and/or near infrared excitation light, and (c) detecting the presence of the single nucleotide polymorphism in the KRAS gene by detecting the detectable change in fluorescence emission wavelength of the metal nanoclusters compared to the wild-type control.