Patent ID: 9284527
Filing Date: 2016-03-15
CPC Classification: A61K,A61Q,C12N

Claim Text:
1. A method of whitening skin using a stem cell conditioned medium, comprising: applying at least 2.5 wt. % of the stem cell conditioned medium to the skin of a subject in need thereof so as to decrease tyrosinase activity and melanin synthesis thereby whitening the skin; and wherein said stem cell conditioned medium is produced by (a) culturing human Wharton's jelly mesenchymal stem cells in a cell culture dish containing a complete growth medium, wherein the complete growth medium includes α-MEM, fetal bovine serum, and human-basic fibroblast growth factors; and (b) sub-culturing the Wharton's jelly mesenchymal stem cells in the complete growth medium and transferring the stem cells to a basal medium to obtain said stem cell conditioned medium, wherein the basal medium includes α-MEM and human-basic fibroblast growth factors.