Patent ID: 9238821
Filing Date: 2016-01-19
CPC Classification: A01N,C07K,C12N

Claim Text:
1. A transgenic legume plant comprising a heterologous nucleic acid sequence that increases expression of flavone synthase or isoflavone synthase, wherein synthesis of medicarpin or 7,4′-dihydroxyflavone is up-regulated in the roots of the plant relative to an otherwise identical plant not comprising the heterologous nucleic acid sequence, wherein expression of the heterologous nucleic acid sequence is up-regulated in response to infection by a fungal plant pathogen or a fungal mutualist, wherein the heterologous nucleic acid sequence comprises a promoter selected from the group consisting of root-preferred promoter and fungal-inducible promoter, wherein the promoter is operably linked to a sequence selected from the group consisting of: a) a nucleic acid sequence encoding the polypeptide of SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:7, or SEQ ID NO:9; b) a nucleic acid sequence comprising the sequence of SEQ ID NO:3; SEQ ID NO:6, or SEQ ID NO:8; c) a nucleic acid sequence having at least 98% sequence identity to the nucleic acid sequence of SEQ ID NO:3; SEQ ID NO:6, or SEQ ID NO:8 and which encodes a polypeptide having flavone synthase or isoflavone synthase activity; and d) a nucleic acid sequence encoding a polypeptide having at least 90% sequence identity to SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:7, or SEQ ID NO:9 and having flavone synthase or isoflavone synthase activity.