Patent ID: 9434898
Filing Date: 2016-09-06
CPC Classification: C10L,C11B,C12N,C12P,Y02E

Claim Text:
1. A process of making an algal biomass comprising at least 67% total fat comprising culturing an algae in two or more types of culture medium sequentially, and wherein said culturing comprises: culturing the algae in a culture medium comprising a carbon source, yeast extract, a magnesium source, and a calcium source; and subsequently, culturing the algae in a culture medium comprising a carbon source, a yeast extract, a nitrogen source, a phosphate source, a magnesium source, a calcium source, and 4 g/L or less of sodium chloride, wherein the nitrogen and phosphate are in a ratio of 50:1 to 4:1, and wherein the type of algae is selected from the group consisting of Thraustochytrium, Schizochytrium, and Aurantiochytrium.