Patent ID: 9249460
Filing Date: 2016-02-02
CPC Classification: C12Q,Y02A

Claim Text:
1. A method for obtaining a sequence, the method comprising: ligating adapters to a plurality of nucleic acid fragments in a sample, each nucleic acid fragment having a length between 5 kilobases and 40 kilobases; quantifying the amount of nucleic acid fragments present in the sample to which adapters have been ligated on each end, thereby quantifying the amount of amplifiable nucleic acid fragments; determining a dilution factor based on the quantified amount of amplifiable nucleic acid fragments; partitioning the sample based on the dilution factor such that each partitioned portion comprises, on average, at least two amplifiable nucleic acid fragments that are a subset of unique sequences; performing a polymerase chain reaction on the partitioned nucleic acid fragments; sequencing the nucleic acid fragments to obtain sequence reads; and obtaining a sequence from the reads.