Patent ID: 9279163
Filing Date: 2016-03-08
CPC Classification: C12N,C12P,C12Y

Claim Text:
1. An isolated cellobiohydrolase enzyme comprising a modified Family 7 catalytic domain, the modified Family 7 catalytic domain comprising one or more amino acid substitution selected from the group consisting of: X46A, X46L, X46T, X51I, X52R, X52W, X53A, X53M, X53R, X53W, X54S, X54I, X54D, X75S, X87T, X93V, X95L, X95Y, X102R, X111T, X129S, X130N, X130E, X139E, X139M, X139Q, X139S, X139R, X143L, X143G, X144A, X144V, X150N, X181L, X183N, X184S, X197L, X197V, X197Q, X197W, X219S, X237T, X241L, X241R, X241V, X253R, X260D, X264C, X264Y, X271I, X343L, X351R, X353M, X374V, X375A, X378E, X379C, X379E, X382L, X382Q, X382I, X383S, X385I, X385L, X390A, X390G, X390K, X390W, X390C, X390L, X390V, X406P, and X419F, wherein the modified Family 7 catalytic domain comprises an amino acid sequence that exhibits from about 80% to about 99.9% identical to amino acids 1-436 of SEQ ID NO: 1 or to amino acids 1-438 of SEQ ID NO: 2, and wherein the isolated cellobiohydrolase enzyme exhibits a. increased specific activity, b. reduced inhibition by glucose, c. reduced inactivation by lignin, d. increased activity in the presence of lignin, e. increased activity in the presence of lignocellulose hydrolysate, or f. any combination of a through e, relative to a cellobiohydrolase comprising a corresponding parental Family 7 catalytic domain from which the modified Family 7 catalytic domain is obtained.