Patent ID: 9346722
Filing Date: 2016-05-24
CPC Classification: A23L,A61K,A61P,A61Q,B01D,C07C,C11B,C12P,Y02E,Y02P

Claim Text:
1. A method for preparing a composition with a squalene content above 95 wt % produced by fermentation of a microalgae belonging to the family Thraustochytriales, wherein said method comprises the following steps: a) preparing a biomass of said microalgae, b) treating the biomass so as to obtain a crude oil containing at least 10 wt % of squalene, c) optionally refining the crude oil thus obtained by a sequence of steps of degumming, deacidification, bleaching and deodorizing, d) extracting the squalene by “short-path” molecular distillation so as to obtain a light fraction having a squalene content above 60 wt %, e) refining this light fraction by a sequence of steps of saponification, two-phase separation, washing, bleaching and deodorizing, so as to obtain a raffinate having a squalene content between 95 and 100%, and f) collecting the squalene composition thus obtained.