Patent ID: 9506935
Filing Date: 2016-11-29
CPC Classification: G01N

Claim Text:
1. A method for estimating the quantity of an analyte contained in a liquid, wherein the method comprises introducing the liquid into a fluid chamber; mixing the liquid with a bi-specific reagent, the bi-specific reagent being configured for grafting on both a particle and an analyte present in the liquid; lighting the fluid chamber using an excitation beam emitted by a light source, the beam extending through the fluid chamber; acquiring at least one image using a matrix photodetector, the image being formed by radiation transmitted by the lighted fluid chamber; and estimating, from at least one acquired image, the quantity of said analyte in the liquid; wherein the method further comprises: computing an histogram for a predetermined region of the acquired image, the histogram representing the number of pixels for each intensity value; and calculating an indicator for said acquired image, said indicator being dependent on the computed histogram, wherein said histogram includes at least one peak being a darkest peak corresponding to the lowest intensity values, said indicator being a function of the most represented intensity value of the darkest peak; and wherein the quantity of analyte is estimated according to said indicator.