Patent ID: 9316587
Filing Date: 2016-04-19
CPC Classification: C12Q,G01N

Claim Text:
1. A process for detecting qualitatively or quantitatively the presence of a single-stranded or double-stranded nucleic acid of interest in a sample, said process comprising the steps of: (a) providing (b) forming a reaction mixture comprising (i), (ii) and (iii) above; (c) contacting under hybridization conditions said first nucleic acid primer with one strand of said nucleic acid of interest; (d) extending said first oligonucleotide primer to form a primer-extended nucleic acid sequence; (e) synthesizing a second nucleic acid strand complementary to said primer-extended nucleic acid sequence or a portion thereof, thereby forming a double-stranded nucleic acid that comprises said phage RNA promoter; transcribing from said RNA promoter in said double-stranded nucleic acid formed in step (e) with a cognate phage RNA polymerase to incorporate said first and second ribonucleotides (iii) into transcripts; (g) detecting the presence or quantity of said nucleic acid of interest by means of energy transfer between said first energy transfer element and said second energy transfer element;