Patent ID: 9422543
Filing Date: 2016-08-23
CPC Classification: C12N

Claim Text:
1. A method for isolating a nucleic acid, which comprises: (i) binding the nucleic acid to a solid phase at a first pH in the presence of a binding buffer, wherein the first pH is an acidic pH; (ii) washing the bound nucleic acid with a wash solution; and (iii) eluting the nucleic acid from the solid phase at a second pH which is higher than the first pH; wherein the wash solution comprises a buffer with a buffering range that encompasses a pH that is higher than the first pH, and the wash solution is at a pH that is within a buffering range of the binding buffer but lower than the buffering range of the buffer of the wash solution, wherein the pH of the wash solution is pH 6.0 or less, and wherein the method is carried out in the absence of an organic solvent.