Patent ID: 9500641
Filing Date: 2016-11-22
CPC Classification: A61K,A61P,C07K,C12N,G01N,Y10S

Claim Text:
1. A vertebrate cell comprising a light-activated ion pump polypeptide that when expressed in a mammalian excitable cell and contacted with a red light silences the excitable cell and the light-activated ion pump polypeptide is fused to one or more cell trafficking signal polypeptides that direct the location of the light-activated ion pump polypeptide expressed in the mammalian excitable cell; wherein the light-activated ion pump polypeptide comprises an amino acid sequence set forth herein as SEQ ID NO:2, with one, two, or more amino acid sequence modifications and has at least 70% amino acid identity to the sequence set forth as SEQ ID NO:2; and wherein the light-activated ion pump polypeptide sequence comprises one or more of: a) a K→R, K→H, or K→Q substitution at an amino acid residue corresponding to amino acid 200 of the amino acid sequence of Halo57 (SEQ ID NO:2); b) a T→S substitution at an amino acid residue corresponding to amino acid 111 of the amino acid sequence of Halo57 (SEQ ID NO:2); c) a T→S substitution at an amino acid residue corresponding to amino acid 203 of the amino acid sequence of Halo57 (SEQ ID NO:2); or d) a K→Q +W→F double substitution at the amino acid residues corresponding to amino acid 200 and 214, respectively, of the amino acid sequence of Halo57 (SEQ ID NO:2).