Patent ID: 9518925
Filing Date: 2016-12-13
CPC Classification: G01J,G01N

Claim Text:
1. A multi-colour luminescence detection system in a microfluidic system, said detection system comprising: a first high gain semiconductor optical sensor, for each luminescence colour component from a cellular or biological sample in the microfluidic system, adapted to be biased at an optimal operating voltage or gain for detecting low light levels of luminescence emitted from the cellular or biological sample with high resolution; a second, optimally biased, high gain semiconductor optical sensor for each colour component adapted to detect light levels of luminescence higher than the levels of luminescence detected by the first high gain semiconductor optical sensor emitted from the cellular or biological sample with high resolution; a splitter for splitting light into at least a first light path and a second light path for each detected colour of luminescence, the first light path coupling the light to the first high gain semiconductor optical sensor and the second light path coupling the light to the second high gain semiconductor optical sensor, wherein the split ratio of the splitter is adapted to attenuate the light in the second light path; and a processor for processing per luminescence colour by combining and weighting the outputs from the first and second high gain semiconductor optical sensors using a signal processing unit, and providing an instantaneous multi-colour high resolution detection system over an extended dynamic range; wherein the first and second high gain semiconductor optical sensors comprise a SiPM sensor.