Patent ID: 9295689
Filing Date: 2016-03-29
CPC Classification: A61K,A61P,C07K,C12N,C12P

Claim Text:
1. A method of producing a protein of interest in a cell comprising contacting the cell with a PLGA microsphere particle consisting essentially of PLGA and having no amine-containing polymers or amino acids, said PLGA microsphere particle comprising a modified mRNA greater than 30 nucleotides-in length encoding said protein of interest, wherein said modified mRNA consists of nucleoside modifications where 25% of uridine residues are replaced with the nucleoside modification 2-thiouridine and 25% of cytosine residues are replaced with the nucleoside modification 5-methylcytosine and wherein said modified mRNA is formulated in the PLGA microsphere particle such that the PLGA microsphere has a modified mRNA percent encapsulation efficiency of at least 68.1%, a particle size of at least 25 μm and an actual RNA loading of at least 0.31 weight percent.