Patent ID: 9512451
Filing Date: 2016-12-06
CPC Classification: C12P

Claim Text:
1. A process for production of fatty acids, sn-regio mono-acylglycerol (MAG), sn-regio diacyl-glycerols (DAG), and glycerol, comprising: preparing a homogenous mixture of fat, polar organic solvent, and water, wherein the homogenous mixture is prepared by mixing the fat, a polar organic solvent and water in a ratio of 1:4:0.15 to 1:7:0.5 under conditions to form a single phase system; subjecting the homogenous mixture to a first enzymatic hydrolysis with an immobilized lipase to obtain a partial hydrolysate comprising fatty acids, MAG, DAG, and glycerol; subjecting the partial hydrolysate to an ion exchange resin to obtain a first product comprising MAG and DAG; and subjecting the first product to a second enzymatic hydrolysis with the immobilized lipase to obtain a second product comprising fatty acids, glycerol, and less than 5% MAG, wherein said polar organic solvent is selected from the group consisting of t-butanol, iso-amyl alcohol, di-acetone alcohol, ethanol, propanol and t-pentanol.