Patent ID: 9518275
Filing Date: 2016-12-13
CPC Classification: C12N,C12P,C12Y

Claim Text:
1. A genetically engineered microbe strain with enhanced secretion of pyruvate, wherein said microbe strain overexpresses wild type dihydrolipoamide acetyltransferase or dihydrolipoamide acetyltransferase mutant which has mutation at its conservative active site, which is selected from the group consisting of wild type dihydrolipoamide acetyltransferase of SEQ ID NO: 1 or SEQ ID NO: 2, dihydrolipoamide acetyltransferase mutant H409A or D413A of SEQ ID NO: 1, and dihydrolipoamide acetyltransferase mutant H442A or D446A of SEQ ID NO: 2.