Patent ID: 9260751
Filing Date: 2016-02-16
CPC Classification: C12Q

Claim Text:
1. A liquid composition comprising a plurality of microemulsions forming aqueous compartments wherein at least a portion of said aqueous compartments comprise: a bead; a polynucleotide template; and oligonucleotide primers for amplifying said template; wherein at least a portion of the oligonucleotide primers is bound to the bead, wherein said microemulsions comprise an oil phase and an aqueous phase, wherein the aqueous phase comprises from 10-30% (v/v) of the microemulsions and the oil phase comprises from 70-90% (v/v) of the microemulsions; wherein the oil phase comprises one or more low viscosity hydrocarbons with a viscosity less than 20 mPas at 25° C. in an amount from 60-85% (v/v) of the oil phase, one or more high viscosity hydrocarbons having a viscosity of greater than 20 mPas at 25° C. in an amount from 10-30% (v/v), and an emulsifier in an amount from 5-10% (v/v).