Patent ID: 9453045
Filing Date: 2016-09-27
CPC Classification: B01D,C07K

Claim Text:
1. A process of purifying the Growth Factor Protein Granulocyte Colony Stimulating Factor (G-CSF) in a purification sequence employing chromatography comprising performing at least one chromatography step using a multimodal resin which comprises a negatively charged 2-(benzoylamino) butanoic acid ligand, binding the G-CSF to the multimodal resin at a pH from 4 to 6.2, and eluting the G-CSF at a pH greater than 6.3, wherein the elution is performed with an arginine buffer having a concentration in the range of from 0.1 M to 2.0 M, optionally in combination with an affinity ligand chromatography step wherein the affinity ligand is a yeast derived Fab fragment directed toward the G-CSF.