Patent ID: 9267143
Filing Date: 2016-02-23
CPC Classification: C12N

Claim Text:
1. A process of transiently expressing a sequence of interest in a plant, plant part, or plant cell culture, comprising: transforming a plant, plant part, or plant cell culture with a heterologous DNA having a sequence encoding an RNA replicon operably linked or linkable to a transcription promoter, wherein said sequence encoding an RNA replicon contains wherein said sequences for replicon function comprise within A/U-rich localities of the coding region of the RNA-dependent RNA polymerase insertion of one or more nuclear introns, whereby A/U-rich localities are sequence stretches of at least 20 nucleotides in length with at least 55% A/U-content or sequence stretches of 6-19 nucleotides in a row of purely A/U-containing sequences.