Patent ID: 9387478
Filing Date: 2016-07-12
CPC Classification: B01L,C12Q

Claim Text:
1. A micro-fluidic device, comprising: a substrate having a top surface and a backside defining a thickness thereof; a trench extending through an entirety of the thickness of the substrate; a plurality of heaters on the substrate for heating the substrate, the plurality of heaters defining a plurality of temperature regions having distinct temperatures, wherein the trench exists between the temperature regions thereby thermally isolating the plurality of heaters on either sides of the trench along a length of the substrate; a flow feature layer defining a channel above the substrate between a fluid inlet and outlet disposed at ends of the length of the substrate and having an intermediate portion, the channel extending across the substrate through each temperature region, wherein the channel resides under a cover defining a serpentine path in the intermediate portion having a plurality of cycles along an extent of the serpentine path, wherein the cycles pass through each of the disposed in the cycles along the extent of the serpentine path in the intermediate portion; and at least one pump between the fluid inlet and the intermediate portion of the channel but not disposed in any of the temperature regions for pumping fluid in the channel to flow from one temperature region to a next temperature region to allow the fluid to undergo thermal cycling.