Patent ID: 9248180
Filing Date: 2016-02-02
CPC Classification: A61K,A61P,C12N,Y02A

Claim Text:
1. A method of producing a pharmaceutical composition in an aqueous mixture form, wherein the pharmaceutical composition comprises a peptide with the amino acid sequence KLKL 5 KLK (SEQ ID NO:1) and an oligodeoxynucleotide with the nucleic acid sequence (dIdC) 13 (SEQ ID NO:2) wherein the peptide and the oligodeoxynucleotide are present as stable complexes, comprising mixing the peptide and the oligodeoxynucleotide, wherein: the peptide is present at a concentration of at least 100 nmol/mL and the oligodeoxynucleotide is present at a concentration of at least 4 nmol/mL, the molar ratio of the peptide to the oligodeoxynucleotide is between 20:1 and 50:1, the mean particle size of the stable complexes comprising the peptide and the oligodeoxynucleotide is less than 1 μm, and applying an energy input to the aqueous mixture, wherein the energy input is applied as a heating step to 40° C. to 60° C. or as a combination of a heating step and homogenization and/or sonication; and wherein: optionally, the composition comprises sodium ions at a concentration from 0 to 25 mM, optionally, the composition comprises Ca optionally, the composition comprises a buffer system or a combination of buffer systems, with a pH of 5.5-9.5, optionally, the composition has a viscosity of less than 15 cP, and optionally, the composition is sterile.