Patent ID: 9399015
Filing Date: 2016-07-26
CPC Classification: A61K,A61P,A61Q,Y10S

Claim Text:
1. A method for making a nanolipidic particle (NLP) population, comprising the steps of: a. preparing a hydrous monophasic precursor solution having phospholipids having phosphatidylcholine (PC), a first concentration of an aqueous solution and a first concentration of non-aqueous solvent selected from ethanol, 1-propanol and 2-propanol; b. adding to said hydrous monophasic precursor solution one or more passenger molecules selected from the group consisting of lipophilic and amphipathic molecules to form a loaded vehicle population of passenger-loaded particles; c. diluting said loaded vehicle population with one or more dilutions of a second concentration of non-aqueous solvent at a ratio of about 1 part passenger-loaded particles to about 20 parts solvent to about 1 part passenger-loaded particles to about 0.5 parts solvent to form a passenger-loaded solvent diluted precursor solution wherein the step of diluting with said non-aqueous solvent is conducted prior to the step of adding a second concentration of said aqueous solution, and d. adding a second concentration of said aqueous solution to an aliquot of said loaded particle population to form an NLP assembly population having a mean particle diameter from about 20 to 300 nm, said NLP assembly population retaining a concentration of said non-aqueous solvent from about 0.5% to about 14%.