Patent ID: 9249439
Filing Date: 2016-02-02
CPC Classification: C12N,C12P

Claim Text:
1. A method of cultivating capsulated bacteria for production of capsular polyoses (CP), wherein said method comprises: (a) providing an inoculum of a strain of bacteria expressing the CP; (b) cultivating the strain in a culture medium to which a feed medium is added by fed-batch fermentation at a preset pH range of 6.9-7.3, whereby an alkali mixture is added to the culture medium at a rate equivalent to that of the feed medium for maintaining the pH range, and wherein the alkali mixture comprises sodium hydroxide and sodium carbonate at a ratio of 3:1; and c) fermenting the culture medium at 35-38° C. under stirring at 50-150 RPM with an air flow rate of 0.1-0.5 vvm.