Patent ID: 9493778
Filing Date: 2016-11-15
CPC Classification: C07K,C12N

Claim Text:
1. A method for enhancing homologous recombination in a population of bacteria of the class Clostridia comprising combinatorially expressing in the bacteria at least one of recombinant RecO with RecU, recombinant RecA with RecU, or recombinant RecG with RecU, thereby producing recombinant Clostridia bacteria, wherein: (a) a site-specific single-crossover chromosomal integration occurs at a greater frequency in the recombinant Clostridia bacteria than in Clostridia bacteria lacking recombinant RecU, recombinant RecA, recombinant RecO and recombinant RecG; (b) a site-specific double-crossover chromosomal integration occurs at a greater frequency in the recombinant Clostridia bacteria than in Clostridia bacteria lacking recombinant RecU, recombinant RecA, recombinant RecO and recombinant RecG; or (c) the homologous recombination frequency is greater in the recombinant Clostridia bacteria than in Clostridia bacteria that does not overexpress recombinant RecU.