Patent ID: 9429518
Filing Date: 2016-08-30
CPC Classification: C12N,C12Q,C12Y,G01N

Claim Text:
1. A homogenous method for real-time determination of deoxynucleoside kinase activity in a sample consisting of: a) contacting the sample, in a container, with a reaction mix comprising a DNA-dependent DNA polymerase, at least one natural deoxynucleoside, at least one of cytidylate kinase and/or dTMP kinase, nucleoside-diphosphate kinase, and a detection system comprising a DNA template molecule, a DNA primer molecule, and a fluorescent moiety capable of being incorporated in, displaced from, or bound to dsDNA synthesized by said DNA-dependent DNA polymerase; b) closing and incubating said container; c) measuring the signal from the fluorescent moiety during primer extension; and d) correlating the signal from the fluorescent moiety to the deoxynucleoside kinase activity in the sample, wherein the method does not include any temperature cycling shifts.