Patent ID: 9316641
Filing Date: 2016-04-19
CPC Classification: G01N

Claim Text:
1. A method of evaluating a subject for presence of JC virus antibodies, said method comprising: a. contacting a biological sample obtained from a subject with highly purified VP1 particles (HPVLPs) in solution under conditions suitable for binding of a JC Virus (JCV) antibody in the sample to an HPVLP, thereby providing a pre-incubated sample; b. contacting the pre-incubated sample with HPVLPs immobilized on a solid substrate under conditions suitable for binding of a JCV antibody in the sample to an HPVLP; c. detecting the level of JCV antibody in the pre-incubated sample binding to the immobilized HPVLPs; and d. comparing the detected level of JCV antibody in the pre-incubated sample binding to the immobilized HPVLPs to a reference value, which corresponds to a detected level of JCV antibody in a pre-incubated control sample, wherein the pre-incubated control sample was pre-incubated in the absence of HPVLPs in solution, thereby providing a pre-incubated control sample, and wherein the pre-incubated control sample was contacted with HPVLPs immobilized on a solid substrate under conditions suitable for binding of a JCV antibody in the sample to an HPVLP thereby evaluating the subject for presence of JC virus antibodies.