Patent ID: 9353409
Filing Date: 2016-05-31
CPC Classification: C12Q

Claim Text:
1. A reagent mixture comprising a ready to use reagent solution, wherein the solution comprises: (a) trehalose in a concentration between 7% and 22%; (b) a viral reverse transcriptase in a concentration sufficient for use in a reverse transcription reaction without adding additional reverse transcriptase, wherein the viral reverse transcriptase is selected from the group consisting of Avian Myeloblastosis Virus Reverse Transcriptase, Respiratory Syncytial Virus Reverse Transcriptase, Moloney Murine Leukemia Virus Reverse Transcriptase, Human Immunodeficiency Virus Reverse Transcriptase, Equine Infectious Anemia Virus Reverse Transcriptase, Rous-Associated Virus 2 Reverse Transcriptase, Avian Sarcoma Leukosis Virus Reverse Transcriptase, RNaseH (−) Reverse Transcriptase, SuperScript II Reverse Transcriptase, and ThermoScript Reverse Transcriptase; and (c) at least one DNA polymerases selected from the group consisting of Thermus Aquaticus, Thermus Thermophilus, Thermus Filiformis, Thermus Brockianus, Thermus Phage, Thermotogo Neapolitana, Pyrococcus Furiosus, Pyrococcus Woesei, Vent, Deep Vent, Kodakaraensis, and mutants thereof; and (d) at least one random primer or at least one oligo deoxy-thymine primer or both random primer and oligo-thymine primer, in a buffer suitable for use in a reverse transcription reaction, wherein the buffer comprises: a co-factor metal ion necessary for reverse transcriptase activity; and nucleoside triphosphates.