Patent ID: 9353165
Filing Date: 2016-05-31
CPC Classification: C07K,C12N,C12Q

Claim Text:
1. A method of separating a colorant from a cell culture derived alpha 1 proteinase inhibitor (recA1PI), wherein the colorant is co-purified with the recA1PI from the cell culture, the method comprising: (a) incubating a sample with a reducing agent, wherein the sample comprises the recA1PI and the colorant, wherein the reducing agent is selected from the group consisting of cysteine and dithiothreitol, whereby a mixture of the recA1PI, the colorant and the reducing agent is obtained, and (b) removing the recA1PI from the colorant and the reducing agent in the mixture of step (a), whereby the colorant is separated from the recA1PI.