Patent ID: 9279770
Filing Date: 2016-03-08
CPC Classification: C12Q,G01N

Claim Text:
1. A method for detecting presence or absence of a target in a sample, the method comprising: contacting a nucleic acid capture probe attached to an addressable location on a solid infrared absorbing surface with the sample under conditions effective to form a hybridization complex between the nucleic acid capture probe and the target; conjugating the hybridization complex to a nanoparticle to form a conjugated hybridization complex; binding silver to the conjugated hybridization complex to provide silver enhancement of the conjugated hybridization complex; exposing the solid infrared absorbing surface containing the conjugated hybridization complex to light having a wavenumber of 1400 cm determining, in external reflection mode, any reflectance from the solid infrared absorbing surface, wherein measurable mid-infrared reflectance indicates the presence of the target in the sample.