Patent ID: 9504719
Filing Date: 2016-11-29
CPC Classification: A61K,A61P,C12N

Claim Text:
1. A method of making a cell lysate comprising lysing cells of a homogeneous umbilical cord cell population isolated from human umbilical cord tissue substantially free of blood, wherein said cell population self-renews and expands in culture, provides trophic support to a soft tissue cell, and has the following characteristics: a) secretes MCP-1, IL-6, IL-8, GCP-2, HGF, KGF, FGF, HB-EGF, BDNF, TPO, MIP1b, RANTES, and TIMP1; b) production of CD10, CD13, CD44, CD73, CD90, PDGFr-alpha, PD-L2 and HLA-A,B,C; c) lacks production of CD31, CD34, CD45, CD80, CD86, CD 117, CD141, CD178, B7-H2, HLA-G, and HLA-DR,DP,DQ; d) has increased expression, relative to a human cell that is a fibroblast, a mesenchymal stem cell, or an iliac crest bone marrow cell, of interleukin 8, reticulon 1 and chemokine (C-X-C motif) ligand 3; and e) has the ability to undergo at least 40 population doublings in culture, wherein a cell lysate is produced.