Patent ID: 9417246
Filing Date: 2016-08-16
CPC Classification: A61P,G01N

Claim Text:
1. A method for measuring the level of the Insulin Receptor Substrate 1 (IRS 1) protein in a human biological sample of formalin fixed tissue, comprising detecting and quantifying the amount of an IRS 1 fragment peptide in a protein digest prepared from said biological sample using mass spectrometry; and calculating the level of IRS 1 protein in said sample; wherein said protein digest comprises a protease digest, wherein the tissue is obtained from a tumor, wherein said IRS 1 fragment peptide has the sequence set forth in SEQ ID NO:76, wherein quantifying said IRS 1 fragment peptide comprises determining the amount of said IRS 1 fragment peptide in a biological sample by comparison to an added internal standard peptide of known amount, wherein said IRS 1 fragment peptide in the biological sample is compared to an internal standard peptide having the same amino acid sequence, wherein the internal standard peptide is an isotopically labeled peptide, and wherein said level of IRS1 protein in said biological sample is an absolute level.