Patent ID: 9487752
Filing Date: 2016-11-08
CPC Classification: A61K,A61P,C12N

Claim Text:
1. A method for producing human neural cells, comprising: a) culturing a population of human pluripotent stem cells in a medium comprising transforming growth factor β (TGFβ) and basic fibroblast growth factor (bFGF) that maintains cell pluripotency; b) priming the pluripotent stem cells, prior to aggregate formation, in an adherent culture and in a serum-free culture medium essentially free of externally added TGFβ and bFGF and in the absence of murine feeder cells; wherein priming occurs for at least one day; and wherein the levels of TGFβ and bFGF are gradually reduced; c) forming aggregates from the cells in step b) in a suspension culture; and d) further differentiating the aggregates into a cell population comprising neural cells, thereby producing human neural cells.