Patent ID: 9448237
Filing Date: 2016-09-20
CPC Classification: C07C,C07F,C12Q,G01N

Claim Text:
1. A method for detecting hydrolase enzyme activity in a sample, said method comprising the step of: contacting said sample with one or more modified tyrosine amino acids according to formula IV wherein Y is chosen from —PO y 2− with y equal to 2 or 3 and esters thereof; and wherein X 1 and/or X 2 is selected from —N 3 , —NO 2 , —Br, or H, wherein either X 1 or X 2 is not H; or contacting said sample with one or more synthetic peptides comprising between 2 and 100 amino acids, characterized therein that said peptide has incorporated therein at least one of said modified tyrosine amino acids detecting the product of the hydrolase enzyme activity on said amino acids or peptides, and optionally detecting the inhibition of hydrolase enzyme activity on said amino acids or peptides.