Patent ID: 9523088
Filing Date: 2016-12-20
CPC Classification: C12N,C12Y

Claim Text:
1. An isolated polynucleotide encoding a modified protease, wherein the polynucleotide comprises a first polynucleotide sequence 5′ and operably linked to a second polynucleotide sequence, wherein the first polynucleotide sequence encodes a modified pro sequence of the protease and the second polynucleotide sequence encodes a mature sequence of the protease, wherein the amino acid sequence encoded by the first polynucleotide sequence comprises at least 95% sequence identity to SEQ ID NO:7, SEQ ID NO:15 or SEQ ID NO:246 and comprises an amino acid substitution at amino acid position E6 of SEQ ID NO:7, SEQ ID NO:15 or SEQ ID NO:246, wherein the substitution of E6 is selected from the group consisting of E6D, E6I, E6S, E6N, E6K, E6H, E6Q and E6R, and the second polynucleotide sequence encodes a mature sequence of the protease, wherein the amino acid sequence encoded by the second polynucleotide comprises at least 70% amino acid sequence identity with a mature protease comprising an amino acid sequence of SEQ ID NO:8, 16 or 247.