Patent ID: 9348127
Filing Date: 2016-05-24
CPC Classification: G01N,G02B

Claim Text:
1. A method for high-resolution imaging of a sample labeled with a fluorescent dye by means of a microscope, the method comprising: illuminating the sample sequentially in a plurality of phases by structured, pulsed excitation light; and recording fluorescent light emitted by the sample for each phase in a respective structured individual image; reconstructing a result image of the sample with enhanced resolution from the individual images; wherein an optimal adjustment is determined for at least one parameter of the illumination and/or at least one parameter of the recording, and wherein a pulse sequence of the illumination is varied pixel by pixel based on intermediate images of the fluorescent light recorded with a higher frame rate than the individual images, and the result image is reconstructed after intensity correction of the individual images is carried out based on the variation of the pulse sequence.