Patent ID: 9321825
Filing Date: 2016-04-26
CPC Classification: A61K,A61P,B01D,C07K,G01N

Claim Text:
1. An N-terminal modified PEG (Polyethylene glycol)-TRAIL (tumor necrosis factor-related apoptosis-inducing ligand) conjugate consisting of: a trimeric TRAIL comprising SEQ ID NO:2 amino acids 114 to 281 and a zipper amino acid motif present at the N-terminus of each TRAIL monomer; and a PEG or a derivative thereof selected from the group consisting of methoxypolyethylene glycol succinimidyl propionate, methoxypolyethylene glycol N-hydroxysuccinimide, methoxypolyethylene glycol aldehyde, methoxypolyethylene glycol maleimide and multiple-branched polyethylene glycol, wherein the PEG is bound via a linker having an average molecular weight between one and 30,000 daltons to an amino acid residue in SEQ ID NO:2 amino acids 114 to 281 or the zipper motif in the N-terminal domain of at least one monomer of the trimeric TRAIL, and wherein the PEG or the derivative thereof and linker has a molecular weight of between 5,000 and 100,000 daltons as determined by SDS-PAGE.