Patent ID: 9493773
Filing Date: 2016-11-15
CPC Classification: C07B,C07H,C12N

Claim Text:
1. A method for preparing a modified ribo-oligonucleotide that contains deuterium comprising the steps of: providing a ribose structure; converting said ribose structure to an α/β ribofuranoside; incorporating deuterium at one or more positions of said α/β ribofuranoside to form a deuterated α/β ribofuranoside having at least one deuterium at a 2 prime position, one deuterium at a 3 prime position, and 2 deuterium at a 5 prime position; incorporating a plurality of benzoyl groups at one or more positions of said deuterated α/β ribofuranoside structure; incorporating an acetate ion at one position of said deuterated α/β ribofuranoside structure; incorporating a nitrogenous base structure to said deuterated α/β ribofuranoside structure, thereby forming a first deuterated nucleoside; providing at least a second a ribose structure; converting said second ribose structure to a second α/β ribofuranoside; incorporating deuterium at one or more positions of said second α/β ribofuranoside to form a second deuterated α/β ribofuranoside having at least one deuterium at a 2 prime position, one deuterium at a 3 prime position, and 2 deuterium at a 5 prime position; incorporating a plurality of benzoyl groups at one or more positions of said second deuterated α/β ribofuranoside structure; incorporating an acetate ion at one position of said second deuterated α/β ribofuranoside structure; incorporating a nitrogenous base structure to said second deuterated α/β ribofuranoside structure, thereby forming a second deuterated nucleoside; and chemically linking said first deuterated nucleoside with said second deuterated nucleoside.