Patent ID: 9410183
Filing Date: 2016-08-09
CPC Classification: C12Q,G01N

Claim Text:
1. A method of detecting the presence of O-GlcNAc (O-linked β-N-acetvlglucosamine) modification of a peptide or protein comprising sequential steps of: a) combining the peptide or protein with EOGT (extracellular O-GlcNAc transferase) or OGT (O-GlcNAc transferase) and UDP-GlcNAc (Uridine 5′-diphospho-N-acetylglucosamine) to produce a first reaction product; b) combining the first reaction product with PAP c) removing background labeling with a glycosidase; d) separating the e) detecting isotopic emissions from the