Patent ID: 9249412
Filing Date: 2016-02-02
CPC Classification: C12N

Claim Text:
1. A method of genetic and epigenetic reprogramming of biological cells, which reprograms at least one cell without returning it to its embryonic state, said method comprising: (a) providing at least one cell, wherein said cell is a stem cell or an adult differentiated cell isolated from a donor tissue; (b) introducing into the cytoplasm of said cell an siRNA specific for a Heterochromatin protein 1α (HP1α), an siRNA specific for a Heterochromatin protein 1β (HP1β), and an siRNA specific for a Heterochromatin protein 1γ (HP1γ), to thereby decrease expression of said HP1α, HP1β and HP1γ, wherein the introduction of said siRNAs specific for HP1α, HP1β and HP1γ rejuvenates said cell by modifying the biological age of said cell without causing functional de-differentiation of said cell, with said cell remaining as a specialized functional cell that is immunologically autologous to said donor tissue, and wherein the rejuvenated cell exhibits a decrease in Senescence-Associated Heterochromatin Foci (SAHF) markers and/or Senescence-Associated Secretory Phenotype (SASP) components, as compared to the cell before rejuvenation.