Patent ID: 9421279
Filing Date: 2016-08-23
CPC Classification: A61K,A61P,C12N

Claim Text:
1. A process for making a DNA preparation, comprising: (a) providing gram-negative bacteria containing said DNA and replicating said DNA in said gram-negative bacteria; (b) lysing said bacteria to obtain a lysate, wherein the lysate is a DNA-containing fraction; (c) filtering the lysate to obtain a filtrate and fractionating said filtrate by gel filtration; (d) chromatographing any DNA-containing fraction on an anion exchanger; and thereafter; (e) chromatographing said DNA-containing fraction on hydroxylapatite, (f) eluting said hydroxylapatite in step (e) with a solution of phosphate, citrate, sulfate or divalent metal ions in order to obtain said DNA preparation, wherein said DNA preparation comprises a bacterially produced DNA, has proteins in an amount of less than about 0.1% DNA, and endotoxins in an amount of less than about 1 EU/mg DNA; wherein the DNA preparation is free of ethidium bromide, phenol, cesium chloride, octylphenolpoly(ethylene glycol ether)