Patent ID: 9404156
Filing Date: 2016-08-02
CPC Classification: C12Q,G16B

Claim Text:
1. A method for obtaining genomic copy number information from genomic nucleic acids, comprising: a) obtaining segments of the genomic nucleic acids; b) tagging the segments with substantially unique tags, thereby generating tagged nucleic acid molecules, such that each of the tagged nucleic acid molecules comprises one segment of the segments of the genomic nucleic acids from step (a) and a substantially unique tag having a unique nucleotide sequence of the substantially unique tags, so as to permit one substantially unique tag having a unique nucleotide sequence of the substantially unique tags to differentiate from another tag having a unique nucleotide sequence of the substantially unique tags; c) subjecting the tagged nucleic acid molecules to a polymerase chain reaction (PCR), thereby generating amplified tagged nucleic acid molecules; d) generating tag associated sequence reads by sequencing the amplified tagged nucleic acid molecules of step (c); e) assigning each of the amplified tagged nucleic acid molecules to a location on a genome associated with the genomic nucleic acids by mapping the subsequence of each tag associated sequence read of said tag associated sequence reads of step (d) corresponding to one segment of the segments of the genomic nucleic acids to a location on the genome; and f) in the amplified tagged nucleic acid molecules, counting the number of the amplified tagged nucleic acid molecules having a different substantially unique tag that have been assigned to the same location on the genome, thereby obtaining counts of the amplified tagged nucleic acid molecules having a different substantially unique tag that have been assigned to the same location of the genome and the genomic copy number information from the genomic nucleic acids, wherein the different substantially unique tag is from the substantially unique tags, and wherein the genome has multiple locations.