Patent ID: 9415368
Filing Date: 2016-08-16
CPC Classification: B01J,C12Q

Claim Text:
1. An assembly for creating nucleic acid cluster arrays, the assembly comprising: a flow cell having a bottom side and a top side that face in opposite directions, the flow cell including an inlet hole and an outlet hole along the top side and a flow channel that fluidicly couples the inlet and outlet holes, the flow channel being defined by a surface that includes a plurality of oligonucleotides immobilized thereto for nucleic acid cluster generation; and a disposable manifold that is separable from the flow cell, the manifold including a main body configured to be mounted onto the flow cell, the main body comprising an outer side and an under side that face in opposite directions, the under side including an input port and an output port that align with the inlet hole and the outlet hole of the flow cell, respectively, when the under side is mounted onto the top side of the flow cell during the nucleic acid cluster generation, the manifold also including an input tube and an output tube that are coupled to the main body and in flow communication with the input and output ports, respectively, wherein fluid flows from the input tube, through the flow channel, and through the outlet tube during the nucleic acid cluster generation.