Patent ID: 9273350
Filing Date: 2016-03-01
CPC Classification: C12Q

Claim Text:
1. A detection system for detection of a template nucleic acid wherein the detection system comprises a primer which comprises, in 3′ to 5′ order; wherein the tail further comprises at least two regions of internal hybridization; wherein said tail is not copiable; wherein the linker comprises a blocking group; wherein said template nucleic acid to be detected has a first target region and a second target region; wherein said first binding region is complementary to and binds to said first target region on said nucleic acid to be detected; wherein said second binding region has the same sequence as the second target region and binds to the complement of said second target region; wherein said primer is extendible when bound to said first target region to produce a primer extension product which comprises a sequence complementary to said second target region sequence and which primer extension product binds to said second binding region; wherein the detection system further comprises an integral signaling system provided on the primer molecule that indicates binding of said primer extension product to said second binding region; and wherein in the absence of the primer extension product or the presence of the primer extension product bound to the template nucleic acid, the at least two internal hybridization regions hybridize with each other to stabilize said integral signaling system in a given position, wherein in the presence of the primer extension product, wherein the primer extension product is not bound to the template nucleic acid, the second binding region of the primer hybridizes to the primer extension product and stabilizes the integral signaling system in a given position.