Patent ID: 9458431
Filing Date: 2016-10-04
CPC Classification: C12M,C12N

Claim Text:
1. A method of propagating stem cells in suspension culture in vitro, the method comprising: (i) attaching stem cells to a plurality of microcarriers to form microcarrier-stem cell complexes, wherein the surface of the microcarriers is coupled to poly-L-lysine and is coated in laminin or vitronectin; (ii) prior to agitation, culturing the microcarrier-stem cell complexes under static culture conditions, such that stem cell-microcarrier aggregates are formed; and iii) culturing the microcarrier-stem cell complexes in suspension culture, wherein the suspension culture is subject to at least 24 hours continuous agitation, wherein the number of stem cells in the culture is thereby expanded, wherein stem cells in the culture after step (iii) are pluripotent or multipotent.