Patent ID: 9453198
Filing Date: 2016-09-27
CPC Classification: A61P,C12N,G01N

Claim Text:
1. A kit comprising a first signaling inhibitor, a second signaling inhibitor and a third signaling inhibitor, wherein said first inhibitor is capable of lowering transforming growth factor beta (TGFβ)/Activin-Nodal signaling, said second inhibitor is capable of lowering Small Mothers Against Decapentaplegic (SMAD) signaling and said third inhibitor is capable of lowering glycogen synthase kinase 3β (GSK3β) for activation of wingless (Wnt) signaling, further comprising instructions for inducing directed differentiation of a pluripotent stem cell into a differentiated neural crest lineage cell or a neuronal lineage cell, in vitro, comprising (i) contacting said stem cell with said first and said second inhibitors for up to 96 hours and (ii) contacting said stem cell with said third inhibitor for up to 240 hours.