Patent ID: 9315860
Filing Date: 2016-04-19
CPC Classification: C12Q

Claim Text:
1. A method for enzymatic synthesis of one or more nucleic acid chains, comprising the step of enzymatic incorporation of nucleotides into the complementary strand of at least one target sequence or equivalents thereof, wherein at least one of the nucleotides is a nucleotide-conjugate having the structure: (Nuc-linker)n- marker wherein: Nuc is a nucleotide (nuc-component); Linker is a linker component, wherein the linker links the nuc-component to a macromolecular marker component; Marker is a macromolecular marker component which comprises at least one target domain having an oligonucleotide sequence complementary to a portion of the target sequence or equivalents thereof; and n is a positive integer from 1 to 1000; and wherein binding of the at least one target domain of the nucleotide-conjugate to the complementary portion of the target sequence facilitates selective incorporation of the said nucleotide-conjugate into the synthesized strand of the one or more nucleic acid chains.