Patent ID: 9493829
Filing Date: 2016-11-15
CPC Classification: C12Q

Claim Text:
1. A method for determining a nucleic acid sequence in a double-stranded nucleic acid molecule, said method comprising: a) providing a sample containing said double-stranded nucleic acid molecule comprising said nucleic acid sequence; wherein: b) completely denaturing the double-stranded nucleic acid molecule of step a) by applying a magnetic field above the sample and generating a tension on the double-stranded nucleic acid molecule, thereby producing a completely denatured double-stranded nucleic acid molecule, wherein said completely denatured double-stranded nucleic acid molecule is a completely extended single-stranded nucleic acid molecule; c) measuring the distance (Z d) hybridizing a known single-stranded nucleic acid primer with said completely denatured double-stranded nucleic acid molecule obtained in step b); e) decreasing the tension to a value such that renaturation and reformation of the hairpin from said completely denatured double-stranded nucleic acid molecule is prevented and a new nucleic acid strand can be synthesized; f) synthesizing said new nucleic acid strand by incubating a product obtained in step e) with a polymerase and a reaction mix comprising: g) determining a position of said pause with respect to one end of the double-stranded nucleic acid molecule, the determining step comprising: h) disassembling the new nucleic acid strand, wherein the sequence of said double-stranded nucleic acid molecule is determined based on the position of said pause and the composition of the dNTPs or/and the ddNTP of the reaction mix of step f).