Patent ID: 9272003
Filing Date: 2016-03-01
CPC Classification: A61F,A61K,A61L,A61P,Y10T

Claim Text:
1. A method for preparing a dehydrated tissue graft, comprising the steps: a) obtaining a placenta comprising an amnion and a chorion; b) separating the amnion from the chorion; c) washing and cleaning the amnion and optionally the chorion, wherein the amnion comprises an epithelial cellular side and a fibroblast cellular side; d) substantially removing all of the epithelial cells from the epithelial cellular side of the amnion while retaining fibroblast cells in the fibroblast cellular side; e) forming a multilayered graft by placing the amnion or the chorion on a surface to form a first layer and then placing one or more additional layers over the first layer to produce a multilayered tissue graft, wherein at least one layer comprises the amnion with the epithelial cells substantially removed while retaining fibroblast cells, and wherein the one or more additional layers is not Wharton's jelly; and f) dehydrating the multilayered tissue graft.