Patent ID: 9506049
Filing Date: 2016-11-29
CPC Classification: C07K,C12N,C12P,Y02E

Claim Text:
1. A method for degrading or converting a cellulosic material, comprising: treating the cellulosic material with an enzyme composition comprising a GH61 polypeptide having cellulolytic enhancing activity at a temperature of about 40° C. to about 70° C., wherein the cellulosic material is pretreated and wherein the GH61 polypeptide having cellulolytic enhancing activity is selected from the group consisting of: (a) a polypeptide comprising an amino acid sequence having at least 90% sequence identity to amino acids 26 to 253 of SEQ ID NO: 2; (b) a polypeptide encoded by a polynucleotide that hybridizes under very high stringency conditions with the full-length complement of nucleotides 76 to 832 of SEQ ID NO: 1, wherein very high stringency conditions are defined as prehybridization and hybridization at 42° C. in 5×SSPE, 0.3% SDS, 200 mg/ml sheared and denatured salmon sperm DNA, and 50% formamide for 12 to 24 hours, and washing three times each for 15 minutes using 2×SSC, 0.2% SDS at 70° C.; (c) a polypeptide encoded by a polynucleotide comprising a nucleotide sequence having at least 90% identity to nucleotides 76 to 832 of SEQ ID NO: 1; and (d) a fragment of SEQ ID NO: 2 having cellulolytic enhancing activity.