Patent ID: 9388456
Filing Date: 2016-07-12
CPC Classification: C12Q

Claim Text:
1. A method for performing a stringent wash step in a hybridization application comprising: a) providing a hybridization product comprising a first nucleic acid sequence hybridized to a second nucleic acid sequence within a cell having a sample morphology, b) providing an aqueous composition for performing a stringent wash, said composition comprising at least one polar aprotic solvent in an amount effective to denature non-complementary sequences in a hybridization product, a hybridization solution, and 5%-40% dextran sulfate, wherein the polar aprotic solvent is not dimethyl sulfoxide (DMSO) and has lactone, sulfone, sulfite, or carbonate functionality, and c) combining the hybridization product and the aqueous composition at a temperature of 82° C. or less for at least a time period sufficient to denature any non-complementary binding between the first and second nucleic acid sequences within the cell such that the sample morphology is preserved.