Patent ID: 9528988
Filing Date: 2016-12-27
CPC Classification: G01N

Claim Text:
1. A method of characterizing a subject to obtain a prognosis or a diagnosis wherein the subject is selected from a group consisting of a transplant candidate, a transplant recipient, an autoimmune patient, and an allergy patient, the method comprising: analyzing, in a sample that comprises proteins present in blood or tissue from the subject, respective amounts of antibodies directed to different antigens, consisting of peptides or amino acid sequences thereof, of a major histocompatibility complex (MHC) protein, wherein the antibodies are immunoglobulins selected from a plurality of MHC binding subclasses and isotypes, wherein the analyzing comprises contacting the sample with types of beads, each of the types of beads having immobilized thereto one of the different antigens of MHC, wherein each of the types of beads is differentially distinct from each of the other types of beads, to produce complexed beads (a), contacting the complexed beads (a) with a plurality of unlabeled monoclonal anti-subject immunoglobulin antibodies, wherein the monoclonal anti-subject immunoglobulin antibodies specifically bind one of the plurality of selected MHC binding subclasses and isotypes on the complexed beads (a) present in the sample, to produce complexed beads (b), contacting the complexed beads (b) with a detectably-labeled antibody that specifically binds to the plurality of unlabeled monoclonal anti-subject immunoglobulin antibodies on the complexed beads (b), to produce complexed beads (c), and for the complexed beads (c), multi-parametrically detecting with a device amounts of the detectably-labeled antibody bound to each of the differentially distinct types of beads, wherein the amount of label associated with each of the differentially distinct types of bead provides an indication of the amount of each of the plurality of selected MHC binding subclasses and isotypes of antibodies in the sample, wherein a distribution of the amounts of the plurality of selected MHC binding subclasses and isotypes in the sample provides an indication of the prognosis of transplant suitability or the diagnosis of allergy or autoimmune disease of the subject.