Patent ID: 9404125
Filing Date: 2016-08-02
CPC Classification: A01K,C07K,C12N,C12P

Claim Text:
1. An isolated B cell of a transgenic animal that uses gene conversion for developing its primary antibody repertoire, wherein said B cell secretes an antibody comprising a heavy and a light chain that are naturally paired by the immune system of the transgenic animal, and wherein the transgenic animal comprises a genome comprising a recombinant immunoglobulin light chain (IgL) locus comprising: a) a functional (IgL) gene comprising a nucleic acid encoding a light chain variable region comprising: b) a plurality of pseudogenes that encode light chain variable regions each comprising: wherein said plurality of pseudogenes are operably linked to said functional IgL gene and donate nucleotide sequences to the nucleic acid of a) by gene conversion in said transgenic animal; and