Patent ID: 9512463
Filing Date: 2016-12-06
CPC Classification: C12Q,G01N

Claim Text:
1. A method for assaying enzymatic activity of one or more lysosomal enzymes, comprising: (a) contacting a sample with a first solution to provide a solution comprising one or more lysosomal enzymes; (b) adding an enzyme substrate for each lysosomal enzyme to be analyzed to the solution comprising the enzymes and incubating the substrates with the enzymes in an enzyme reaction solution for a time sufficient to provide a solution comprising an enzyme product for each lysosomal enzyme present in the sample, wherein the enzyme reaction solution comprises: (c) optionally quenching the enzyme reaction; (d) determining the quantities of the enzyme products; and (e) determining the enzymatic activity of the one or more lysosomal enzymes based on the quantities determined in step (d).