Patent ID: 9334520
Filing Date: 2016-05-10
CPC Classification: C12Q,G01N

Claim Text:
1. A method of detecting mycoplasma in a bioprocess manufacturing application that uses recombinant technology to modify materials within bacteria comprising: collecting a sample from a cell culture used by the bioprocess manufacturing application to catalyze a biochemical reaction within a microorganism; controlling a Raman spectrometer, by a processor, to collect a Raman spectrum of a targeted volume within the sample so as to collect a Raman spectrum of a single cell of a known cell line of interest; obtaining a reference spectra uniquely associated with the known cell line, the reference spectra comprising at least two of: spectral measurements of comparing, using a processing device, the reference spectra to the collected spectrum; identifying whether there are unnatural molecular compositions within the collected spectrum based upon the comparison of the reference spectra to the collected spectrum; providing an indication as to whether stopping the bioprocess manufacturing application when