Patent ID: 9296802
Filing Date: 2016-03-29
CPC Classification: A61K,A61P,C07K,C12N,G16B,G16C

Claim Text:
1. A method for preparing an EGFR (Epidermal Growth Factor Receptor) domain 2 specific modified non-antibody protein comprising the steps of: (a) selecting non-antibody proteins having a structural complementarity with the target site of EGFR domain 2 in a non-antibody protein library; (b) calculating a binding energy of the selected non-antibody protein and EGFR domain 2; (c) selecting a non-antibody protein having a favorable binding energy among the selected non-antibody proteins; (d) selecting amino acid residue having high binding energy among the interfacial amino acid residues of the selected non-antibody protein and EGFR domain 2, wherein the selected amino acid residue having high binding energy is selected from 44Lys, 107Glu, 110Glu, 114Asn, and 118Asp; and (e) substituting the amino acid residue selected in step (d) with the amino acid residue having low binding energy, wherein step (e) is performed by phage display and bio-panning.