Patent ID: 9523073
Filing Date: 2016-12-20
CPC Classification: A61K,C07K,C12N,G01N

Claim Text:
1. A method for detecting a soluble Δ9 isoform of the human IL-23 receptor (IL-23Rα) in a biological sample, wherein said Δ9 isoform consists of the amino acid sequence of SEQ ID NO:2, the method comprising the steps of: (a) obtaining a biological sample; (b) immobilizing on a solid support with a capture reagent, said capture reagent comprises a first antibody that binds to said soluble Δ9 isoform of the human IL-23Rα at the amino acid residues 318-348 of SEQ ID NO:2; (c) incubating said biological sample with said immobilized capture reagent to allow said soluble Δ9 isoform of IL-23Rα to bind to said immobilized capture reagent; (d) incubating a second antibody that binds to the extracellular domain of the human IL-23Rα and is coupled with a detecting agent, to allow said second antibody to bind to the captured soluble Δ9 isoform of IL-23Rα; (e) washing said solid support; and (f) adding a detectable reagent to detect the signal generated by the bound second antibody.