Patent ID: 9254318
Filing Date: 2016-02-09
CPC Classification: A61K,A61P,C07K,C12N,C12Y

Claim Text:
1. A plurality of influenza virus vectors for a reassortant, comprising a) a vector for vRNA production comprising a promoter operably linked to an influenza virus PA cDNA linked to a transcription termination sequence, a vector for vRNA production comprising a promoter operably linked to an influenza virus PB1 cDNA linked to a transcription termination sequence, a vector for vRNA production comprising a promoter operably linked to an influenza virus PB2 cDNA linked to a transcription termination sequence, a vector for vRNA production comprising a promoter operably linked to an influenza virus HA cDNA linked to a transcription termination sequence, a vector for vRNA production comprising a promoter operably linked to an influenza virus NP cDNA linked to a transcription termination sequence, a vector for vRNA production comprising a promoter operably linked to an influenza virus NA cDNA linked to a transcription termination sequence, a vector for vRNA production comprising a promoter operably linked to an influenza virus M cDNA linked to a transcription termination sequence, and a vector for vRNA production comprising a promoter operably linked to an influenza virus NS cDNA linked to a transcription termination sequence, wherein the vectors for the reassortant include cDNAs for PB1, PB2, PA, NP, M, and NA with sequences that encode a polypeptide having at least 97% contiguous amino acid sequence identity to a corresponding polypeptide encoded by SEQ ID NOs:1-5 and a polypeptide having at least 90% contiguous amino acid sequence identity to a corresponding polypeptide encoded by SEQ ID NO:8, a cDNA for NS that has a Glu residue at position 55 corresponding to position 55 in SEQ ID NO:38 and encodes a polypeptide having at least 97% contiguous amino acid sequence identity to a corresponding polypeptide encoded by SEQ ID NO:6, and a cDNA for HA that is from a different influenza virus strain than a strain having gene segments with sequences corresponding to the cDNAs for PB1, PB2, PA, NP, M, NS, and NA having SEQ ID Nos. 1-6 and 8, and wherein the cDNA for PB2 has a Tyr at residue 360; and b) a vector for mRNA production comprising a promoter operably linked to a DNA segment encoding influenza virus PA, a vector for mRNA production comprising a promoter operably linked to a DNA segment encoding influenza virus PB1, a vector for mRNA production comprising a promoter operably linked to a DNA segment encoding influenza virus PB2, and a vector for mRNA production comprising a promoter operably linked to a DNA segment encoding influenza virus NP, and optionally a vector for mRNA production comprising a promoter operably linked to a DNA segment encoding influenza virus HA, a vector for mRNA production comprising a promoter operably linked to a DNA segment encoding influenza virus NA, a vector for mRNA production comprising a promoter operably linked to a DNA segment encoding influenza virus Ml, a vector for mRNA production comprising a promoter operably linked to a DNA segment encoding influenza virus M2, or a vector for mRNA production comprising a promoter operably linked to a DNA segment encoding influenza virus NS2, which plurality of vectors, when introduced to an avian or mammalian cell, result in production of the reassortant which is capable of enhanced replication in embryonated eggs and MDCK cells relative to a corresponding influenza virus that does not have a NA with sequences that encode polypeptide having at least 90% contiguous amino acid sequence identity to a corresponding polypeptide encoded by SEQ ID NO:8 or does not have a NS with a Glu residue at position 55 corresponding to position 55 in SEQ ID NO:38 or a PB2 with a Tyr residue at position 360.