Patent ID: 9366685
Filing Date: 2016-06-14
CPC Classification: G01N

Claim Text:
1. A rapid, sensitive method for the detection and quantification of non-naturally occurring drugs for forensic drug testing in a post-mortem human subject using oral fluid from the post-mortem subject, comprising: collecting a sample of oral fluid from a post-mortem human subject; analyzing the oral fluid sample qualitatively using an enzyme-linked immunosorbent assay (ELISA) method to detect the presence of one or more of the non-naturally occurring drugs; analyzing the oral fluid sample quantitatively using a Liquid Chromatography-Mass Spectrometry/Mass Spectrometry (LC-MS/MS) method to determine the concentration of the one or more non-naturally occurring drugs in the post-mortem human subject; and identifying the one or more non-naturally occurring drugs in the post-mortem human subject, wherein detection and quantification in oral fluid is more sensitive and faster than detection and quantification of the non-naturally occurring drugs in blood, urine, bile, and liver tissue collected from the same post-mortem human subject using the LC-MS/MS method and the ELISA method, wherein the one or more non-naturally occurring drugs are selected from the group consisting of amphetamine, 6-acetylmorphine, alprazolam, cocaine/benzoylecgonine, buprenorphine, cannabinoids, carisoprodol, clonazepam, codeine, cyclobenzaprine, diazepam, fentanyl, hydrocodone, hydromorphone, methadone, 3,4-methylenedioxy-methamphetamine (MDMA), methamphetamine, meperidine, morphine, o-desmethyl-cis-tramadol, oxazepam, oxycodone, oxymorphone, propoxyphene, phencyclidine (PCP), tapentadol, temazepam and delta-9-tetrahydrocannabinol (THC), wherein qualitative and quantitative results are obtained in as soon as three hours.