Patent ID: 9228207
Filing Date: 2016-01-05
CPC Classification: A61K,A61P,C12N,C12Y

Claim Text:
1. A switchable single-guide RNA (sgRNA), comprising (1) an sgRNA backbone sequence, wherein the sgRNA backbone sequence comprises a nucleotide sequence that is at least 90% identical to the nucleotide sequence 5′- GUUUUAGAGC UAUGCUGAAA AGCAUAGCAA GUUAAAAUAA GGCUAGUCCG UUAUC -3′(nucleotides 79-133 of SEQ ID NO: 6) and/or at least 90% identical to the nucleotide sequence 5′- GUUUUAGAGC UAUGCUGAAA AGCAUAGCAA GUUAAAAU -3′(nucleotides 22-59 of SEQ ID NO: 9); (2) an RNA aptamer comprising a riboswitch, wherein the aptamer comprises 20-100 nucleotides, and wherein the riboswitch is selected from a theophylline riboswitch, a thiamine pyrophosphate (TPP) riboswitch, an adenosine cobalamin (AdoCbl) riboswitch, an S-adenosyl methionine (SAM) riboswitch, an SAH riboswitch, a flavin mononucleotide (FMN) riboswitch, a tetrahydrofolate riboswitch, a lysine riboswitch, a glycine riboswitch, a purine riboswitch, a GlmS riboswitch, or a pre-queosinel (PreQ1) riboswitch; and (3) a guide sequence that is complementary to a DNA target site,