Patent ID: 9494568
Filing Date: 2016-11-15
CPC Classification: A61D,B01L,C12M,C12N,G01N,Y10T

Claim Text:
1. A passive counter flow free microfluidic device for sorting a sample of flagellated cells by means of the flagellated cells' intrinsic motility difference, the passive microfluidic device comprising: an input reservoir configured to receive the sample of flagellated cells; an output reservoir positioned downstream of the input reservoir, the output reservoir configured to collect the flagellated cells that are sorted; and a plurality of microchannel sections arranged sequentially and in fluid communication with each other between the input reservoir and the output reservoir, and the plurality of microchannel sections designed to define a closed microchannel system between the input reservoir and the output reservoir, wherein each microchannel section comprises at least one microchannel, wherein an inlet of the microchannel of a first microchannel section of the plurality of microchannel sections is connected to the input reservoir, wherein an outlet of the microchannel of a last microchannel section of the plurality of microchannel sections is connected to the output reservoir, and wherein, for each microchannel section, the total volume of the microchannels of one microchannel section of the plurality of microchannel sections, is larger than that of a subsequent microchannel section disposed in the downstream direction, such that the passive microfluidic device is configured to sort the flagellated cells by means of the flagellated cells' intrinsic motility difference when the flagellated cells move through a stationary fluid medium from the input reservoir to the output reservoir.