Patent ID: 9526771
Filing Date: 2016-12-27
CPC Classification: A61K,A61P,C12N

Claim Text:
1. A preparation method of an antigen composition, which comprises the following steps: (1) mixing soluble protein from melanoma cells with soluble protein from lung adenocarcinoma cells, to obtain tumor antigen protein; or mixing melanoma cells with lung adenocarcinoma cells and extracting soluble protein from the mixed cells, to obtain tumor antigen protein; (2) allowing said tumor antigen protein to get into contact with immature dendritic cells; said immature dendritic cells not only have surface marker phenotype characterized by CD11c negative, CD80 negative, CD86 negative, MHC class I H-2K (3) after contacting with tumor antigen protein, inducing the immature dendritic cells into mature dendritic cells; said mature dendritic cells have surface marker phenotype characterized by CD11c positive, CD80 positive, CD86 positive, MHC class I H-2K (4) separating membrane vesicle of said mature dendritic cells.