Patent ID: 9465027
Filing Date: 2016-10-11
CPC Classification: A61P,B01D,G01N

Claim Text:
1. A method for measuring a percent of a neutralizing form of an autoantibody to an anti-TNFα drug in a sample, the method comprising: (a) contacting the sample with a labeled anti-TNFα drug and a labeled TNFα to form: (b) subjecting the first labeled complex and/or the second labeled complex to size exclusion chromatography to separate them from free labeled TNFα, free labeled anti-TNFα drug, and/or a complex of labeled anti-TNFα drug and labeled TNFα; (c) measuring an area under the curve (AUC) of free labeled TNFα in the sample (Free labeled TNFα (d) measuring the percent of a neutralizing form of the autoantibody by comparing the AUC of free labeled TNFα measured in step (c) to an AUC of free labeled TNFα in a first control sample (Free labeled TNFα wherein the first control sample is a reference sample that is normal human serum to which labeled TNFα and labeled anti-TNFα drug has been added, and the second control sample is a reference sample that is normal human serum to which only labeled TNFα has been added.