Patent ID: 9493826
Filing Date: 2016-11-15
CPC Classification: B01F,B01L,C12Q

Claim Text:
1. A method of binary quantification of nucleic acids in a sample, comprising: a. providing a microfluidic device comprising a first substrate comprising a first population of areas and a second substrate comprising a second population of areas; b. partitioning a sample comprising nucleic acid molecules into said first and second populations of areas in said microfluidic device by exerting the sample through a conduit in fluidic communication with each area of the first population of areas and each area of the second population of areas via a continuous fluidic pathway within said first and second substrates, such that a plurality of said first and second populations of areas each comprise only one nucleic acid molecule; c. loading nucleic acid amplification reagents into said microfluidic device; d. effecting relative motion between the first substrate and the second substrate, thereby isolating the first population of areas from the second population of areas; and e. conducting a nucleic acid amplification reaction in said microfluidic device in both said first and second populations of areas, wherein during said nucleic acid amplification reaction the temperature of said sample and the temperature of said nucleic acid amplification reagents remain within 10° C. of their temperature at the beginning of said nucleic acid amplification reaction.