Patent ID: 9290807
Filing Date: 2016-03-22
CPC Classification: C01G,C07H,C12Q

Claim Text:
1. A method of identifying a modified cytosine residue in a sample nucleotide sequence comprising the steps of: (i) providing a population of polynucleotides which comprise the sample nucleotide sequence, (ii) oxidising or reducing a first portion of said population, (iii) treating the oxidised or reduced first portion of said population and a second portion of said population with bisulfite, (iv) sequencing the polynucleotides in the first and second portions of the population following steps (ii) and (iii) to produce first and second nucleotide sequences, respectively, and (v) identifying a residue in the first and second nucleotide sequences which corresponds to a cytosine residue in the sample nucleotide sequence.