Patent ID: 9261460
Filing Date: 2016-02-16
CPC Classification: C12Q,G01N

Claim Text:
1. A composition of matter comprising at least two parts: a first part comprising at least two segments, wherein said first segment comprises a first nucleic acid primer and at least one first energy transfer element, wherein said first energy transfer element is: (a) an energy donor and (b) covalently attached directly or via a linker arm to said first nucleic acid primer and said second segment comprises a primer-extended nucleic sequence complementary to at least a portion of a nucleic acid target sequence; and a second part comprising at least two segments, wherein said first segment of said second part comprises a second nucleic acid primer and at least one second energy transfer element, wherein said second energy transfer element is: (a) an energy acceptor and (b) covalently attached directly or via a linker arm to said first nucleic acid primer; and wherein said second segment of said second part comprises a primer-extended nucleic acid sequence which is identical to at least a portion of said nucleic acid target sequence; wherein said first nucleic acid primer does not comprise said second energy transfer element, and wherein said second nucleic acid primer does not comprise said first nucleic acid energy element; wherein said first primer is not complementary to said second primer or nucleic acid in the absence of a target dependent extension step; and wherein neither of said nucleic acid primers is fixed or immobilized to a solid support.