Patent ID: 9404154
Filing Date: 2016-08-02
CPC Classification: C12Q

Claim Text:
1. A method for replicating a polynucleotide target, comprising the steps of: hybridizing to a polynucleotide target an amplification primer comprising a 3′ target-specific nucleotide sequence and a 5′ non-target-specific universal sequencing tag comprising at least one natural nucleobase that is not target specific and at least three contiguous non-natural nucleobases, each of which is capable of being replicated during amplification, and extending the amplification primer under PCR conditions, thereby producing a polynucleotide product that is complementary to the polynucleotide target and that further comprises a 5′ non-target-specific universal sequencing tag comprising at least one natural nucleobase that is not target specific and at least three contiguous non-natural nucleobases, wherein the at least three contiguous non-natural nucleobases present in the polynucleotide product are not capable of being sequenced and cause termination of primer extension by a sequencing polymerase, whereby the portion of the 5′ non-target-specific universal sequencing tag that is 5′ to the at least three contiguous non-natural nucleobases cannot be sequenced.