Patent ID: 9347935
Filing Date: 2016-05-24
CPC Classification: C07K,G01N

Claim Text:
1. A method for determining a functional effect of a compound upon a cell expressing a first recombinant polypeptide and a second recombinant polypeptide, the method comprising the steps of: (a) expressing the first recombinant polypeptide and the second recombinant polypeptide in a plasma membrane of the cell, (b) assaying a parameter in the cell, the parameter indirectly or directly under the influence of a T1R GPCR protein or a sweet and/or an amino acid taste receptor comprising one or more T1R GPCR proteins wherein the parameter is ligand binding or transduction of a cellular signal in response to ligand binding; (c) contacting the cell with the compound; (d) assaying the cell for an increase or decrease in the parameter, thereby determining the functional effect, if any, of the compound.