Patent ID: 9393324
Filing Date: 2016-07-19
CPC Classification: A61K

Claim Text:
1. A method of determining cardiac toxicity of a compound of interest, comprising the steps of a. optionally, i) determining from a heart or cardiac cell of a mammal peak levels of one or more indicators of cardiac energetics selected from inorganic phosphate, phosphocreatine, ATPγ, or a combination thereof; and ii) one or more indicators of cardiac function selected from contractility, relaxation, heart rate, conduction velocity, or a combination thereof, before administration of said compound of interest to the heart or cardiac cell; b. contacting said heart or cardiac cell of a mammal with said compound of interest; c. measuring after step (b), i) peak levels of said one or more indicators of cardiac energetics after administration of said compound of interest to the heart or cardiac cell of the mammal; and, optionally, ii) one or more indicators of cardiac function selected from contractility, relaxation, heart rate, conduction velocity, or a combination thereof; d. contacting said heart or cardiac cell of said mammal previously contacted with said compound of interest, after step (c) with an agent known to cause an increase in cardiac function, said cardiac function selected from contractility, relaxation, heart rate, conduction velocity, or a combination thereof; e. measuring after step (d), i) peak levels of said one or more indicators of cardiac energetics after administration of said agent known to cause an increase in cardiac function; and, optionally ii) one or more indicators of cardiac function selected from contractility, relaxation, heart rate, conduction velocity, or a combination thereof; f. comparing the levels of said one or more indicators obtained in step (c) with the levels of said one or more indicators obtained in step (e);