Patent ID: 9447188
Filing Date: 2016-09-20
CPC Classification: C07K,C12N,C12P

Claim Text:
1. A method of controlling quality and quantity of posttranslational modification of a recombinantly produced polypeptide/protein (glycoprotein), wherein the posttranslational modification affects the glycosylation profile and/or the acidic variants profile, as manifested in CEX profiles, wherein the glycosylation profiles are selected from bG0 structures and bG1 structures, and wherein the polypeptide/protein (glycoprotein) production is in eukaryotic host cells, the method comprising the following steps: a) cultivating the eukaryotic cells in a suitable medium under conditions which allow the expression of the polypeptide/protein, wherein the content of the dissolved CO b) increasing the content of the dissolved CO wherein the amount of bG0 structures is increased and the amount of bG1 structures is decreased, relative to the amounts obtained by a method with no pCO2 regulation.