Patent ID: 9388465
Filing Date: 2016-07-12
CPC Classification: C12N,C12Q,G01N

Claim Text:
1. A method of synthesizing a library of polynucleotides comprising barcode sequences, said method comprising: synthesizing a plurality of polynucleotides comprising barcode sequences; separating said polynucleotides into a plurality of partitions, thereby generating partitioned polynucleotides, wherein each partition comprising a polynucleotide comprises multiple copies of the same polynucleotide comprising a barcode sequence prior to amplifying said partitioned polynucleotides; amplifying said partitioned polynucleotides, thereby generating a library of amplified polynucleotides, comprising at least about 100,000 different barcode sequences; wherein said amplified polynucleotides are attached to a bead; and isolating partitions comprising amplified polynucleotides.