Patent ID: 9382582
Filing Date: 2016-07-05
CPC Classification: C12Q

Claim Text:
1. A method comprising: a) contacting a mixed sample comprising a major template and a minor template with a first oligonucleotide primer and a second oligonucleotide primer, the second oligonucleotide primer is labeled and comprises a modification, the modification comprising a 4-oxo-tetradecyl group or a 2-(N-formyl-N-methyl)aminotheyl group or both, the first and second oligonucleotides primers priming amplification of a same target species, the target species being a short tandem repeat (STR); b) performing a nucleic acid amplification reaction and thereby forming a reacted mixed sample; c) subjecting the reacted mixed sample to capillary electrophoresis; and d) detecting and comparing a labeled product from the reacted mixed sample to an allelic ladder, the label attributable to an amplicon from a minor template being relatively greater than in an amplification reaction comprising the first oligonucleotide primer and the second oligonucleotide primer without a 4-oxo-tetradecyl group or a 2-(N-formyl-N-methyl)aminotheyl group or both.