Patent ID: 9271996
Filing Date: 2016-03-01
CPC Classification: A61K,A61P,C07K,C12N,C12P

Claim Text:
1. A method of producing a protein of interest in a cell comprising contacting the cell with a PLGA microsphere particle, said PLGA microsphere particle consisting essentially of PLGA and having no amine-containing polymers or amino acids, said PLGA microsphere particle comprising a modified mRNA greater than 30 nucleotides in length encoding said protein of interest, wherein said modified mRNA consists of a nucleoside modification which is N1-methylpseudouridine in place of uridines, wherein the PLGA microsphere particle has a percent encapsulation efficiency of at least 68.1%, a particle size of at least 40 um and an actual RNA loading of at least 0.31 weight percent.