Patent ID: 9410186
Filing Date: 2016-08-09
CPC Classification: C12Q,G01N

Claim Text:
1. A method for assessing the binding affinity of a substrate to a nucleic acid using a nucleic acid probe that comprises a fluorescent moiety linked to a nucleic acid binding moiety through a linker with the binding moiety capable of binding to the nucleic acid, the method comprising, incubating the nucleic acid with the probe at a predetermined ratio to form a complex between the nucleic acid and the probe, wherein the complex formation elicits a first florescence intensity change in the probe; incubating the complex with the substrate to elicits a second fluorescence intensity change in the probe; and comparing the first intensity change with the second intensity change for assessment of the binding affinity of the substrate to the nucleic acid, wherein the fluorescence intensity changes due to a pKa shift of the fluorescent moiety.