Patent ID: 9528105
Filing Date: 2016-12-27
CPC Classification: B01L,C12N,C12Q,G01N

Claim Text:
1. A method for treating a biological sample for nucleic acid extraction, the method comprising: obtaining a biological sample, the biological sample comprising one or more of stool, blood, saliva, or urine; diluting the biological sample in an extraction buffer comprising 0.5 to 20 wt. % organic solvent to form a mixture; incubating the mixture at a temperature from 15° C. to 35° C. for five seconds to thirty minutes, to cause nucleic acid present in the biological sample to be extracted from at least a portion of the biological sample for further processing; separating at least a portion of the extraction buffer and at least a portion of the nucleic acid from the biological sample; forming an amplification mixture comprising: the at least a portion of the extraction buffer; the at least a portion of the nucleic acid; and one or more amplification reaction buffers; and incubating the amplification mixture for 1 to 10 minutes at 25° C. to 70° C.