Patent ID: 9322055
Filing Date: 2016-04-26
CPC Classification: C12Q,G01N

Claim Text:
1. A method for quantification of a target nucleic acid in a sample, the method comprising: forming a plurality of discrete sample portions, each of the plurality of discrete sample portions comprising a portion of the sample, and a reaction mixture; amplifying the plurality of discrete sample portions to form a plurality of discrete processed sample portions including at least one discrete processed sample portion containing nucleic acid amplification reaction products; detecting fluorescence signals from the at least one of the plurality of discrete processed sample portions to determine a presence of the at least one target nucleic acid; determining the respective volumes of the plurality of discrete processed sample portions, wherein the determining the respective volumes comprises imaging the plurality of discrete processed sample portions; and estimating the number of copies-per-unit-volume of the at least one target nucleic acid in the sample based on the number of discrete processed sample portions determined to contain the at least one target nucleic acid therein.