Patent ID: 9315815
Filing Date: 2016-04-19
CPC Classification: C12N

Claim Text:
1. A method of high-throughput sorting of protein-expression cells, comprising the following steps: (a) constructing a vector comprising a recombinant nucleotide sequence with a detectable label, which comprises, in 5′ to 3′ direction, a first sequence encoding a protein or a polypeptide of interest, a second sequence encoding a self-processing cleavage site and a third sequence encoding an extracellular-transmembrane domain, wherein the second sequence is located between the first sequence and the third sequence, and wherein the detectable label is located at the first sequence or the third sequence; (b) transfecting a cell with the vector of (a); (c) adding a self-processing cleavage enzyme inhibitor so that the recombinant protein encoded by the recombinant sequence of the vector of (a) can be transiently anchored on a cell surface; and (d) determining the detectable label to sort the recombinant protein expressing cells that express anchored recombinant protein on the cell surface to obtain protein-expression cells.