Patent ID: 9518273
Filing Date: 2016-12-13
CPC Classification: C12N,C12P,C12Y

Claim Text:
1. A method of producing butadiene from a fermentable carbon source, the method comprising: a.) providing a fermentable carbon source; b.) contacting the fermentable carbon source with a microorganism comprising one or more polynucleotides coding for enzymes in a pathway that catalyzes a conversion of the fermentable carbon source to one or more intermediates in a pathway for the production of butadiene, and one or more polynucleotides coding for enzymes in a pathway that catalyze a conversion of the one or more intermediates to butadiene in a fermentation media; and c.) expressing the one or more polynucleotides coding for the enzymes in the pathway that catalyzes a conversion of the fermentable carbon source to one or more intermediates in a pathway for the production of butadiene and the one or more polynucleotides coding for enzymes in a pathway that catalyze a conversion of the one or more intermediates to butadiene in the microorganism to produce butadiene, wherein the one or more intermediates in the pathway for the production of butadiene is crotonyl-CoA, and wherein all of the enzymes are required from either (i) crotonyl-CoA reductase (bifunctional) (E.C. 1.1.1), and crotonyl alcohol dehydratase (E.C. 4.2.1, 4.2.1.127) or (ii) crotonaldehyde dehydrogenase (E.C. 1.2.1), crotonyl alchol dehydrogenase (E.C. 1.1.1, 1.1.1.1), and crotonyl alcohol dehydratase (E.C. 4.2.1, 4.2.1.127).