Patent ID: 9334534
Filing Date: 2016-05-10
CPC Classification: C07H,C12P,C12Q

Claim Text:
1. A process for the addition of a nucleotide having, after incorporation, the structure to the 3′-end of an oligonucleotide primer having a preselected sequence, wherein X is selected from the group consisting of —H and —OH, Z is the point of attachment to the 3′-end of said oligonucleotide primer, and B is selected from the group consisting of said process comprising contacting said primer in buffered aqueous solution with a template under conditions where said primer forms a duplex with said template, and incubating said duplex with a mixture of polymerase and polymerase substrates comprising the triphosphate wherein X and B are defined as above, and wherein said added nucleotide is incorporated by said polymerase opposite a non-complementary standard nucleoside in the template selected from the group consisting of adenosine, guanosine, cytidine, uridine, and thymidine.