Patent ID: 9493835
Filing Date: 2016-11-15
CPC Classification: C12Q

Claim Text:
1. A method for identifying the presence of a polymorphic target sequence variant, comprising: (a) combining a sample suspected of containing a first or second variant of a polymorphic target sequence with a labeled probe, one excess reverse primer, and two or more limiting forward primers to generate a combined sample, wherein the limiting forward primers are not perfect matches to the target sequence; (b) treating the combined sample under amplification conditions such that a single stranded amplicon is generated, wherein the single-stranded amplicon comprises: (c) detecting signal from the labeled probe at two temperatures; (d) calculating an experimental signal ratio of the signal at the two temperatures; and (e) comparing the experimental signal ratio with known signal ratios for the first and second variants of the polymorphic sequence, wherein a match between the experimental signal ratio and the known signal ratio for the first or second variant of the polymorphic sequence identifies the presence of said first or second variant.