Patent ID: 9265845
Filing Date: 2016-02-23
CPC Classification: A61K,C07K,G01N

Claim Text:
1. A method of imaging a tissue highly expressing an endothelial integrin that is at least one of αvβ5 integrin, αvβ3 integrin and α5β1 integrin, comprising contacting said tissue with an integrin binding peptide, said integrin binding peptide comprising: (a) a binding sequence specific to bind to at least one of αvβ5 integrin, αvβ3 integrin and α5β1 integrin, said binding sequence further being 11 amino acids long and comprising the sequence RGD; and (b) a knottin scaffold substantially identical to EETI-II except that a portion of the knottin scaffold is replaced with the binding sequence, said integrin binding peptide further having at least 90% identity to a peptide that is one of SEQ ID NO: 23 through SEQ ID NO: 52, inclusive, comprising cysteine disulfide linkages adjacent to the binding sequences, said integrin binding peptide further comprising a label that can be detected when the integrin binding peptide is bound to the tissue, thereby imaging the tissue.