Patent ID: 9453194
Filing Date: 2016-09-27
CPC Classification: B01F,C12M

Claim Text:
1. A method for performing a cell culture growth process, comprising: providing a containment vessel for holding a cell culture medium, the containment vessel comprising a generally semi-cylindrical bottom wall defining a semi-cylindrical concavity therewithin, the containment vessel having a rotatable mixing wheel mounted within for rotation about a horizontal axis approximately coincident with an axis of curvature of the semi-cylindrical bottom wall, the mixing wheel having a rotational diameter that extends into close proximity with the semi-cylindrical bottom wall; sterilizing the containment vessel and the mixing wheel so that at least the interior of the containment vessel and the mixing wheel are sterile; providing a cell culture medium within the containment vessel; providing nutrients within the containment vessel to support growth of a cell culture within the cell culture medium; enclosing the containment vessel such that the cell culture medium is not exposed to the surrounding environment; and rotating the mixing wheel about the horizontal axis to mix the contents of the containment vessel.