Patent ID: 9476094
Filing Date: 2016-10-25
CPC Classification: C12Q

Claim Text:
1. A method for determining the sequence of a polynucleotide, consisting essentially of the sequential steps of: (i) contacting a polynucleotide processive enzyme immobilised in a fixed position having a metal nanoparticle positioned on or proximal to the enzyme with a target polynucleotide to form a complex; (ii) contacting the complex with one or more nucleoside triphosphates selected from the group consisting of dATP, dTTP, dGTP, and dCTP, under conditions sufficient to induce polynucleotide processive enzyme activity; (iii) generating a non-linear optical signal in the area encompassing at least a portion of the immobilized enzyme; and (iv) detecting an effect as the complex interacts with the one or more nucleoside triphosphates, by measurement of the non-linear optical signal, wherein the non-linear optical signal measurement consists of measuring the second or third harmonic signals, thereby determining the sequence of the polynucleotide.