Patent ID: 9435739
Filing Date: 2016-09-06
CPC Classification: C12Q,G01N

Claim Text:
1. A method of detecting a biological activity, comprising: providing, forming a hydrogel comprising the first indicator reagent and the gelling agent; distributing the particles on at least a portion of a major surface of the hydrogel; bringing the sample into fluid communication with a portion of the hydrogel, wherein the first biological derivative partitions from hydrogel onto and/or into the plurality of particles; and detecting a presence or absence of the first biological derivative, wherein the first biological derivative is retained by a particle, wherein the presence of the first biological derivative is indicative of the presence of a first biological activity, wherein detecting the presence of the first biological derivative comprises counting a quantity of discrete fluorescent zones, and wherein the particles are between about 0.05 microns and about 20 microns.