Patent ID: 9255126
Filing Date: 2016-02-09
CPC Classification: A61K,A61P,C07K,C12N,C12P,C12Q

Claim Text:
1. An alphavirus replicon RNA, comprising: (1) a 5′ alphaviral nucleotide sequence required for nonstructural protein-mediated amplification, (2) one or more alphaviral nucleotide sequence(s) encoding nonstructural proteins nsP1, nsP2, nsP3, and nsP4, (3) an alphaviral nucleotide subgenomic promoter sequence, (4) one or more heterologous protein-encoding nucleotide sequence(s), and (5) a 3′ alphaviral nucleotide sequence required for nonstructural protein-mediated amplification, wherein the one or more alphaviral nucleotide sequence(s) encoding the nonstructural proteins is derived from a alphavirus, wherein the alphaviral nucleotide sequence(s) exhibit sequence identity to at least one third but no more than two-thirds of a genome of an alphavisus, and wherein the alphavirus replicon RNA further comprises a modification in a nonstructural protein selected from the group consisting of: (a) deletion of amino acid residues 101-120 of the nsP1 protein, (b) deletion of amino acid residues 450-470 of the nsP1 protein, (c) deletion of amino acid residues 460-480 of the nsP1 protein, (d) deletion of amino acid residues 470-490 of the nsP1 protein, (e) deletion of amino acid residues 480-500 of the nsP1 protein, (f) deletion of amino acid residues 9-29 of the nsP2 protein, (g) deletion of amino acid residues 613-633 of the nsP2 protein (h) deletion of amino acid residues 650-670 of the nsP2 protein, (i) deletion of amino acid residues 740-760 of the nsP2 protein, (j) deletion of amino acid residues 8-28 of the nsP4 protein, and (k) deletion of amino acid residues 552-570 of the nsP4 protein, wherein the amino acid residues are numbered relative to wild-type Venezuelan Equine Encephalitis (VEE) virus encoded by SEQ ID NO:140.