Patent ID: 9410175
Filing Date: 2016-08-09
CPC Classification: A23K,C07K,C12C,C12N,C12P,C12R,C12Y,G01N

Claim Text:
1. A process for the increased production of an enzyme comprising: (a) cultivating a filamentous fungus belonging to the species wherein said cultivating growth phase is conducted in the presence of all filamentous fungal nutrients in an amount sufficient to avoid the establishment of a nutrient limitation in the growth of the filamentous fungus, wherein the filamentous fungal nutrients comprise a carbon source, a nitrogen source as well as additional compounds required for growth of the filamentous fungus and/or the production of the enzyme, wherein said additional compounds comprise phosphate, sulfate, trace elements and/or vitamins and said cultivating production phase is conducted in the presence of all filamentous fungal nutrients except oxygen in an amount sufficient to avoid the establishment of a limitation of nutrients other than oxygen in the production phase, and wherein the cultivating production phase is conducted in the presence of an amount of oxygen suitable to establish and maintain conditions of oxygen limitation during the cultivating production phase, wherein the suitable amount of oxygen is fed to the culture broth during the cultivating production phase to ensure that the oxygen transfer rate (OTR) of the culture broth is identical to the oxygen uptake rate (OUR) of the culture broth with a deviation of plus or minus 10%, and wherein the suitable amount of oxygen is fed to the culture broth to further ensure that the OTR is as high as possible; and (b) recovering from the culture broth the enzyme obtained in the cultivating production phase, said enzyme yield obtained in the cultivating production phase being greater than obtainable by a similar process wherein said cultivating production phase is conducted under conditions such that all nutrients including oxygen are provided to the culture broth in an amount sufficient to avoid the establishment of a limitation of nutrients.