Patent ID: 9285361
Filing Date: 2016-03-15
CPC Classification: G01N

Claim Text:
1. A method for the analysis of circulating antibodies, said method comprising the steps of: a. providing an analysis device comprising a substrate, and provided on said substrate at least one sample addition zone, at least one retaining zone, at least one sink, and at least one flow path connecting the at least one sample addition zone, the at least one retaining zone and the at least one sink, wherein the at least one flow path is open and comprises projections substantially vertical to the surface of said substrate and having a height (H), diameter (D) and reciprocal spacing (t b. binding cells or cell structures onto projections in said at least one retaining zone, said cells or cell structures forming binding surfaces, c. adding a liquid sample to said sample addition zone, d. binding circulating antibodies onto said formed binding surfaces, and e. reading a result, wherein circulating antibodies directed against bound cell structures are determined, in which the projections are defined by a tapered configuration and in which the projections are narrower at the top of the projections than the bottom and in which the reciprocal spacing between projections is larger between the top of adjacent projections than the bottom thereof.