Patent ID: 9249182
Filing Date: 2016-02-02
CPC Classification: B01D,C07K

Claim Text:
1. A method for producing a preparation comprising a protein of interest and a reduced amount of at least one impurity, said method comprising: (a) contacting a sample mixture comprising the protein of interest and the at least one impurity to a hydrophobic interaction chromatography (HIC) media in the presence of a load buffer and collecting a flow through fraction, such that the protein of interest binds to the HIC media at a Kp of at least 90; and (b) contacting said hydrophobic interaction chromatography media with a wash buffer solution having a salt concentration within 20% of the salt concentration of the load buffer and collecting a wash fraction; wherein the flow through and/or wash fractions constitute a preparation comprising a protein of interest and having a reduced amount of the impurity relative to the sample mixture.