Patent ID: 9389219
Filing Date: 2016-07-12
CPC Classification: C12Q,G01N

Claim Text:
1. A method of screening for a candidate compound, wherein said method comprises: contacting the candidate compound with a cell expressing both an aggregation-prone engineered member protein and one or more wild-type member proteins of a tumor suppressor protein aggregome, wherein the tumor suppressor protein aggregome comprises member proteins that form aggregation-specific interactions; and measuring co-aggregation of one or more member proteins of said tumor suppressor protein aggregome and/or the activity of one or more wild-type member proteins of said tumor suppressor protein aggregome expressed by the cell; and screening for a candidate compound that inhibited or disrupted co-aggregation of one or more member proteins of the tumor suppressorrm expressed by the cell, wherein said co-aggregation is mediated by the exposure of a beta-aggregating region present in said member proteins, said beta-aggregating region comprising a peptide that comprises: a stretch of 4 to 16 contiguous amino acids, at least 50% of which are hydrophobic amino acids, and in which at least one aliphatic residue or F is present, and if only one aliphatic residue or F is present, at least one residue selected from the group consisting of Y, W, A, M and T; and in which no more than one P, R, K, D or E residue is present.