Patent ID: 9481895
Filing Date: 2016-11-01
CPC Classification: C12N

Claim Text:
1. A kit for the introduction of modular vector elements comprising: (a) a preference vector, wherein said preference vector comprises a promoter sequence, a selection marker sequence, and a reporter sequence, wherein the preference vector is selected from the group consisting of pGK-GFP, pCAGGS-LUC, SV40-CYN and pGK-RFP and the preference vector further comprises an Ori site and an EM7-Zeo site; and (b) a content vector, wherein said content vector comprises a genomic content of interest, a first LTR sequence that is 5′ of the genomic content of interest and a second LTR sequence that is 3′ of the genomic content of interest, and wherein said content vector does not comprise any promoter sequence and said preference vector does not comprise said genomic content of interest and further wherein said preference vector contains one preference vector site specific recombination sequence and said content vector contains one content vector site specific recombination sequence, wherein the preference vector and the content vector are capable of a single cross-over event at the preference vector site specific recombination sequence and the content vector site specific recombination sequence to form a lentiviral vector, wherein the lentiviral vector comprises the preference vector and the content vector.