Patent ID: 9441030
Filing Date: 2016-09-13
CPC Classification: C07K

Claim Text:
1. A method for producing recombinant Factor VIII from mammalian host cells transformed with an expression DNA vector containing cDNA coding for FVIII or FVIII derivatives in a culture medium and purifying said factor VIII to at least 95% purity using factor VIII specific affinity molecules linked to a solid support, comprising (a) culturing said mammalian host cells in a culture medium, which is supplemented with a sulfated polysaccharide having protease-inhibiting activity, wherein said sulfated polysaccharide is dextran sulfate having a molecular weight of about 20,000 to about 5,000,000 Da, and wherein the amount of said dextran sulfate in said culture medium is 25 mg/L to 200 mg/L; (b) concentrating said culture medium containing factor VIII through ultrafiltration; and (c) purifying said factor VIII from the concentrated culture medium by immunoaffinity chromatography, wherein said chromatography comprises: wherein the factor VIII is purified to at least 95% purity.