Patent ID: 9285319
Filing Date: 2016-03-15
CPC Classification: C07H,C12Q,G01N

Claim Text:
1. A method of detecting formation of a base pair of artificial bases, the method comprising: observing a decrease in fluorescence of an artificial fluorescent base, wherein the decrease in fluorescence of an artificial fluorescent base is caused by formation of a base pair of an artificial fluorescent base and a quenching base, wherein the artificial fluorescent base is selected from the group consisting of: (i) a 7-(2,2′-bithien-5-yl)imidazo[4,5-b]pyridin-3-yl group (Dss); (ii) a 7-(2,2′,5′,2″-terthien-5-yl)imidazo[4,5-b]pyridin-3-yl group (Dsss); (iii) a 2-amino-6-(2,2′-bithien-5-yl)purin-9-yl group (ss); (iv) a 2-amino-6-(2,2′,5′,2″-terthien-5-yl)purin-9-yl group (sss); (v) a 4-(2,2′-bithien-5-yl)-pyrrolo[2,3-b]pyridin-1-yl group (Dsas); (vi) a 4-[2-(2-thiazolyl)thien-5-yl]pyrrolo[2,3-b]pyridin-1-yl group (Dsav); and (vii) a 4-[5-(2-thienyl)thiazol-2-yl]pyrrolo[2,3-b]pyridin-1-yl group (Dvas); and the quenching base having a 2-nitropyrrole structure is represented by Formula III or IV: wherein R 3 is selected from —H, iodine, —CH 3 , and: wherein R 4 is selected from —CH 3 , —CH 2 —NH 2 , and: wherein n is an integer of 0 to 12, and wherein the nitrogen atom at the 2-position of the 2-nitropyrrole structure is attached to a ribose or deoxyribose to form a nucleoside or nucleotide.