Patent ID: 9320761
Filing Date: 2016-04-26
CPC Classification: A61K,A61L,A61P

Claim Text:
1. A method of preparing an osteogenic composition comprising: obtaining a biological sample, said biological sample comprising cells and one or more osteogenic agents, wherein the biological sample comprises whole blood; contacting said biological sample with 0.15 N HCl, thereby lysing the biological sample to produce a lysate, said lysate comprising solid material and one or more osteogenic agents; neutralizing said lysate to a pH of from about 4 to 10 by contacting said lysate with an alkaline composition; separating the lysate into a first fraction and a second fraction, said first fraction substantially comprising the one or more osteogenic agents, and said second fraction substantially comprising the solid material, wherein the solid material of said second fraction including cellular debris and/or precipitated proteins; and collecting said first fraction, thereby obtaining an osteogenic composition having one or more osteogenic agents.