Patent ID: 9506089
Filing Date: 2016-11-29
CPC Classification: C12N,C12P,C12Y,Y02P

Claim Text:
1. A genetically engineered microorganism that produces acrylate, wherein the genetically engineered microorganism comprises: an exogenous polynucleotide encoding a coenzyme A (CoA) transferase catalyzing conversion of 3-hydroxypropionic acid (3-HP) to 3-hydroxypropionyl-CoA (3-HP-CoA) and belonging to EC 2.8.3.8, EC 3.1.2-, or EC 6.2.1.7, an exogenous polynucleotide encoding a 3-HP-CoA dehydratase catalyzing conversion of 3-HP-CoA to acrylyl-CoA and belonging to EC 4.2.1, an exogenous polynucleotide encoding a 3-HP-CoA hydrolase or a 3-hydroxyisobutyryl-CoA hydrolase belonging to EC 3.1.2, a polynucleotide encoding an enzyme catalyzing the conversion of glycerol to 3-propionic aldehyde (3-HPA), and a polynucleotide encoding an enzyme catalyzing the conversion of 3-HPA to 3-HP, and wherein the genetically engineered microorganism has increased expression of the exogenous polynucleotides as compared to a microorganism of the same type that does not comprise the exogenous polynucleotides, wherein the genetically engineered microorganism is an wherein the