Patent ID: 9506056
Filing Date: 2016-11-29
CPC Classification: A61K,A61P,C12N

Claim Text:
1. A method of increasing cellular uptake of a first plurality of nanoparticles relative to a second plurality of nanoparticles, the method comprising: producing the first plurality of nanoparticles comprising a first mixed monolayer on the surface of the nanoparticles, the first mixed monolayer comprising a first oligonucleotide and a first diluent, wherein the first plurality consists essentially of nanoparticles having oligonucleotide functionalized on the nanoparticle at surface density X which is at least about 1.5 picomoles/cm producing the second plurality of nanoparticles comprising a second mixed monolayer on the surface of the nanoparticles, the second mixed monolayer comprising a second oligonucleotide and a second diluent, wherein the second plurality consists essentially of nanoparticles having oligonucleotide functionalized on the nanoparticle at surface density Y which is at least about 0.3 pmol/cm wherein X is greater than Y; contacting a first cell with the first plurality and contacting a second cell with the second plurality; wherein uptake of the first plurality of nanoparticles by the first cell is increased relative to uptake of an equal concentration of the second plurality by a second cell, wherein the first cell and the second cell are of the same type, wherein the first oligonucleotide and the second oligonucleotide do not comprise a cell-specific recognition element, and wherein the nanoparticles in the first plurality of nanoparticles and the nanoparticles in the second plurality of nanoparticles have a mean diameter of from about 5 to about 50 nanometers.