Patent ID: 9408961
Filing Date: 2016-08-09
CPC Classification: A61M,B01D,G16H

Claim Text:
1. A method for washing biological cells comprising: providing a separator comprising a relatively rotatable cylindrical housing and an internal member; wherein said cylindrical housing has an interior surface and said internal member has an exterior surface, said surfaces defining a gap therebetween, wherein at least one of said surfaces includes a porous membrane; introducing cells suspended in a liquid medium into said gap; rotating at least one or both of said housing and said internal member; separating said cells from said liquid medium; concentrating said cells; removing at least some of said concentrated cells from said separator through a first outlet to a first integrally connected in-process container; removing at least some of said separated liquid medium from said separator through a second outlet; resuspending said concentrated cells in the first in-process container with a replacement fluid; introducing said resuspended cells into the gap of said separator; concentrating said cells and removing said concentrated cells to a second integrally connected in-process container; removing at least some of the separated replacement fluid from said separator through said second outlet; determining whether an acceptable volume of concentrated cells has been attained in said second integrally connected container; and transferring said concentrated cells from said second integrally connected in-process container to an integrally connected final product container upon attainment of an acceptable volume of concentrated cells.