Patent ID: 9366672
Filing Date: 2016-06-14
CPC Classification: C12M,C12Q,G01N,Y10S

Claim Text:
1. A method of detecting a microorganism in a sample comprising: (a) providing (1) a biosensor device for detecting the microorganism in the sample comprising a surface covalently bound to a capture agent having a saccharide moiety, (2) the sample, and (3) an unbound lectin capable of multivalently binding to an exposed cell wall of the microorganism and to the saccharide moiety of the capture agent; (b) applying the sample and an amount of an unbound lectin to the surface with the capture agent having the saccharide moiety, for a time to bind the lectin to the saccharide moiety of the capture agent and multivalently bind the lectin to the microorganism to attach the microorganism to the surface; and (c) detecting the microorganism attached to the surface with the biosensor device.