Patent ID: 9464267
Filing Date: 2016-10-11
CPC Classification: C12N

Claim Text:
1. A process for control of multiple target bacteria in a water source comprising the following sequential steps: (a) culturing a first dominant bacteria in a first mixed bacteria solution obtained from the water source, said first mixed bacteria solution containing dominant bacteria and sub-dominant bacteria, isolating a virulent phage for the first dominant bacteria, and lysing the first dominant bacteria from the first mixed bacteria solution to remove the first dominant bacteria from the mixed solution, thereby generating a second mixed bacteria solution lacking the first dominant bacteria; (b) culturing the next dominant bacteria from the second mixed bacteria solution lacking the first dominant bacteria; isolating a virulent phage for the next dominant bacteria and lysing the next dominant bacteria from the second mixed bacterial solution to remove the next dominant bacteria, thereby generating a subsequent mixed bacterial solution lacking both the first and next dominant bacteria; (c) performing the culturing, isolating, and lysing steps on each subsequent mixed bacterial solution to provide a set of bacteriophage that will lyse the first dominant bacteria and multiple subdominant bacteria in the first, second and subsequent mixed bacteria solutions; and (d) applying an effective amount of the bacteriophage of said set either as a mixture or sequentially to the water source.