Patent ID: 11918640
Assignee: THE UNITED STATES OF AMERICA, AS REPRESENTED BY THE SECRETARY, DEPARTMENT OF HEALTH AND HUMAN SERVICES
Field: Pharmaceuticals (Chemistry)
Classification: CPC A  C | IPC A  C

Claim 0:
1. A population of 1.2×1010 to 4.3×1010 HPV-specific T-cells produced by a method comprising:
(a) dividing an HPV-positive tumor sample obtained from a patient into multiple fragments;
(b) culturing the multiple fragments in the presence of at least one cytokine;
(c) obtaining T-cells from the cultured fragments; and
(d) expanding the number of T-cells to produce an expanded population of HPV-specific T-cells using:
one or both of (i) irradiated allogenic feeder cells and (ii) irradiated autologous feeder cells; and
one or both of (iii) OKT3 antibody and (iv) interleukin-2 (IL-2), wherein the T-cells have not been depleted of CD4+ cells.