Patent ID: 11946087
Assignee: AMYRIS BIO PRODUCTS PORTUGAL, UNIPESSOAL, LDA
Field: Biotechnology (Chemistry)
Classification: CPC C  A | IPC A  C

Claim 21:
22. The method for co-production of claim 1, wherein the second isoprenoid is astaxanthin, and the host cell comprises:
(a) at least one of:
(i) a heterologous nucleic acid encoding a phytoene synthase;
(ii) a heterologous nucleic acid encoding a lycopene cyclase; or
(iii) a heterologous nucleic acid encoding a bifunctional enzyme having phytoene synthase and lycopene cyclase activities;

(b) a heterologous nucleic acid encoding a phytoene desaturase;
(c) at least one of:
(i) a heterologous nucleic acid encoding a β-carotene ketolase;
(ii) a heterologous nucleic acid encoding a β-carotene hydroxylase;
(iii) a heterologous nucleic acid encoding a cytochrome p450 hydroxylase and ketolase capable of converting β-carotene to echinenone and subsequently to β-cryptoxanthin and to astaxanthin; or
(iv) a heterologous nucleic acid encoding a cytochrome p450 reductase which interacts with the cytochrome p450 hydroxylase and ketolase.