Patent ID: 11939404
Assignee: AJINOMOTO CO., INC.
Field: Biotechnology (Chemistry)
Classification: CPC C  Y | IPC C

Claim 0:
1. A method for producing a cyclized peptide from a linear peptide, wherein:
the linear peptide comprises two or more SH groups as functional groups,
all SH groups are protected,
an N terminal amino group is optionally protected, and
all of a C-terminal carboxy group and other functional groups on the peptide are protected,
the method comprising:
(1-A) removing protecting groups of all functional groups other than the protected SH groups in the linear peptide;
(1-B) forming a temporary S—S bond to protect all SH groups of the linear peptide;
wherein the peptide obtained from following both (1-A) and (1-B) in any order or both simultaneously is an S-protected peptide,
wherein, in the S-protected peptide:
two or more SH groups are present as the functional groups on the peptide,
all SH groups are protected by formation of a temporary S—S bond, and
all protecting groups of other functional groups on the peptide are removed, and

(2) folding the S-protected peptide under oxidation and reduction conditions to re-form an S—S bond in the S-protected peptide, obtaining the cyclized peptide.