Patent ID: 11896480
Assignee: WISCONSIN ALUMNI RESEARCH FOUNDATION
Field: Medical technology (Instruments)
Classification: CPC A  C | IPC A  C

Claim 8:
9. The method of claim 1, wherein the human VFFs for use in the step (a) are obtained by:
(a) seeding human vocal fold mucosa (HVFM) cells onto an extracellular matrix (ECM)-coated surface, whereby some of the HVFM cells adhere to the ECM-coated surface and some of the HVFM cells remain free-floating and non-adherent to generate HVFM adherent cells and HVFM non-adherent cells; and
(b) culturing the HVFM adherent cells in a fibroblast-oriented medium, resulting in a subpopulation of human VFFs.