Patent ID: 11892397
Assignee: BOARD OF REGENTS, THE UNIVERSITY OF TEXAS SYSTEM
Field: Measurement (Instruments)
Classification: CPC G | IPC G

Claim 10:
11. An endotoxin assay method comprising the steps of providing an analyte sample suspected of containing an endotoxin from lipopolysaccharide cell wall component of Gram-negative bacteria in an analyte; adding the analyte sample to at least one sample well optically coupled to and interfacing with a reflecting surface of a total internal reflection open cavity photonic crystal biosensor optically coupled to an optical prism; adding Limulus Amoebocyte Lysate (LAL) reagent to the analyte sample; exposing the analyte sample in the at least one sample well to a focused probe laser signal across the total internal reflection open cavity photonic crystal biosensor; reflecting an output beam from the photonic crystal biosensor through the optical prism; imaging the output beam; and monitoring changes in a refractive index of the analyte sample.