Patent ID: 11932860
Assignee: KIKKOMAN CORPORATION
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 7:
8. A vector for Aspergillus transformation, comprising:
(a) an open reading frame encoding an alkaline phosphatase (ALP); and
(b) an Aspergillus strain-derived N-terminal secretory signal peptide,
wherein the ALP comprises 90% or greater homology with either of the amino acid sequences set forth in SEQ ID NOs: 31 or 32 and has an amino acid substitution at position 122, 249, 251 and/or 410, wherein the Asn at position 122 is a substitution to Gln or Lys, the Asn at position 249 is a substitution to Gln, Asp or His, the Thr at position 251 is a substitution to Cys, and the Asn at position 410 is a substitution to Gln or Lys,
wherein the Aspergillus strain-derived N-terminal secretory signal peptide is 5′ to the open reading frame encoding the ALP and comprises 90% or greater homology with any one of the sequences set forth in SEQ ID NOs: 2 to 15, and
wherein the ALP has decreased protein glycosylation levels compared to a wild-type ALP.