Patent ID: 11860349
Assignee: UNIVERSITAT DE BARCELONA
Field: Optics (Instruments)
Classification: CPC G | IPC G

Claim 8:
9. A method of operating the confocal microscope of claim 8, comprising
making the light source to emit a first light beam of a certain diameter (D1);
expanding the first light beam into a second light beam having a prescribed diameter (D2) to define an illumination window on the first acousto-optic deflector;
injecting a first hologram into the first acousto-optic deflector to modulate the second light beam and transform it into a third light beam;
imaging the third light beam on the second modulation plane;
collimating the third light beam at zero modulation, so that the diameter (D2) of the third light beam is that of the second light beam and defines an illumination window on the second acousto-optic deflector;
injecting a second hologram into the second acousto-optic deflector, to modulate the third light beam and transform it into a fourth light beam;
imaging the fourth light beam on the input pupil of the microscope objective;
focussing the fourth light beam on a reconstruction plane that intersects the sample; and
collecting the fluorescent light emitted by the sample and focussing said light on the imaging sensor.