Patent ID: 11898159
Assignee: UNIVERSITY OF LOUISVILLE RESEARCH FOUNDATION
Field: Biotechnology (Chemistry)
Classification: CPC C  B | IPC B  C

Claim 4:
5. A pre-vascularized spheroid comprising one or more microvascular fragments surrounding a composition that comprises one or more biologically-relevant materials, said biologically-relevant materials and microvascular fragments being dispersed in a hydrogel comprising alginate, said spheroid having uniform dimensions and a diameter between 500 and 2,500 microns, wherein said spheroid is produced by a method comprising:
providing a droplet of a suspension on a delivery pen tip, wherein the suspension comprises one or more biologically-relevant materials dispersed within a biocompatible medium, wherein the biocompatible medium is a hydrogel comprising alginate;
bringing the droplet into contact with a surface of a salt solution by bringing the delivery pen tip toward the salt solution; and
compressing the droplet against the surface of the salt solution for a period of time until the spheroid is formed;
wherein the biologically-relevant materials comprise stromal vascular fraction cells or stem cells.