Patent ID: 11895962
Assignee: PHYTOTEQ, INC.
Field: Biotechnology (Chemistry)
Classification: CPC A  C | IPC A  C

Claim 0:
1. A method for cultivating agave cells for the product of tequila, comprising:
(a) prepare a callus introduction media including sucrose, naphthalene acetic acid, and water;
(b) place agave cells into the introduction media to incubate a callus formation;
(c) establish a mixture by placing the callus formation into an accelerator media including sucrose, naphthalene acetic acid, and water, supplemented by a vitamin stock solution, and modify the mixture to obtain a pH of between 5.5 and 6;
(d) heat the mixture of (c);
(e) use cultures obtained from the mixture of (d) to cultivate subcultures from accelerator media and supplement with citric acid to obtain a pH of between 3.5 and 4.5, and introduce light to the subcultures;
(f) add carbon dioxide and yeast, and ferment;
whereupon an ethanol concentration is adjusted to produce tequila.