Patent ID: 11951162
Assignee: SK BIOSCIENCE CO., LTD.
Field: Pharmaceuticals (Chemistry)
Classification: CPC A  C | IPC A  C

Claim 6:
7. A method of preparing the immunogenic composition of claim 1, the method comprising:
(a) fermenting and dissolving bacterial cells which produce capsular polysaccharides derived from Streptococcus pneumoniae serotypes 1, 2, 3, 4, 5, 6A, 6B, 7F, 8, 9N, 9V, 10A, 11A, 12F, 14, 15B, 17F, 18C, 19A, 19F, 20, 22F, 23F, and 33F;
(b) purifying the capsular polysaccharides from the dissolved bacterial cells;
(c) activating the capsular polysaccharides with an oxidizing agent; and
(d) combining each of the capsular polysaccharides with a carrier protein to form a Streptococcus pneumoniae capsular polysaccharide-protein conjugate,
wherein the capsular polysaccharides from serotypes 1 and 5 are conjugated to tetanus toxoid and the capsular polysaccharides from serotypes 2, 3, 4, 6A, 6B, 7F, 8, 9N, 9V, 10A, 11A, 12F, 14, 15B, 17F, 18C, 19A, 19F, 20, 22F, 23F, and 33F are conjugated to CRM197,
wherein the capsular polysaccharide from serotype 2 has a molecular weight of 100 to 400 kDa and a degree of oxidation of 2 to 18,
wherein the capsular polysaccharide from serotype 9N has a molecular weight of 200 to 700 kDa and a degree of oxidation of 2 to 19,
wherein the capsular polysaccharide from serotype 17F has a molecular weight of 400 to 900 kDa and a degree of oxidation of 1 to 22, and
wherein the capsular polysaccharide from serotype 20 has a molecular weight of 400 to 800 kDa and a degree of oxidation of 4 to 16.