Patent ID: 11859193
Assignee: NUSEED GLOBAL INNOVATION LTD.
Field: Basic materials chemistry (Chemistry)
Classification: CPC C  A  Y | IPC A  C

Claim 6:
7. A transgenic Sorghum species plant or transgenic Zea mays plant, or a vegetative part thereof, being transgenic for at least (a) a first exogenous polynucleotide which encodes a plant WRI1 polypeptide operably linked to a heterologous promoter which is effective to direct expression of the first exogenous polynucleotide in the Sorghum species plant or Zea mays plant or part thereof and increase the level of the WRI1 polypeptide relative to a corresponding wild type plant or part thereof, and (b) a second exogenous polynucleotide which encodes a plant DGAT polypeptide operably linked to a heterologous promoter which is effective to direct expression of the second exogenous polynucleotide in the Sorghum species plant or Zea mays plant or part thereof and increase the level of the DGAT polypeptide relative to the corresponding wild type plant or part thereof, whereby said vegetative part of said transgenic Sorghum species plant or said vegetative part of said transgenic Zea mays plant comprises a total fatty acid (TFA) content which comprises fatty acids esterified in the form of triacylglycerols (TAG) and fatty acids in the form of lipids other than TAG, wherein said vegetative plant part comprises a TFA content of between 6% and 20% (w/w dry weight) and a TAG/TFA Quotient (TTQ) of between 0.60 and 0.84, or between 0.84 and 0.95, and wherein said transgenic Sorghum species plant or said transgenic Zea mays plant comprising said vegetative plant part comprising one or more of:
(i) an exogenous polynucleotide which encodes an RNA molecule which is complementary to a portion of an mRNA molecule encoding an endogenous SDP1 TAG lipase polypeptide and which down-regulates production of the endogenous SDP1 TAG lipase polypeptide in the Sorghum species or Zea mays vegetative plant part, wherein the exogenous polynucleotide is operably linked to a heterologous promoter which is effective to direct expression of the exogenous polynucleotide in the Sorghum species or Zea mays vegetative plant part and the RNA molecule down-regulates production of the endogenous SDP1 TAG lipase polypeptide relative to a corresponding wild type vegetative plant part;
(ii) an exogenous polynucleotide which encodes a plant LEC2 polypeptide operably linked to a heterologous promoter which is effective to direct expression of the exogenous polynucleotide in the Sorghum species or Zea mays vegetative plant part and increase the level of the LEC2 polypeptide relative to the corresponding wild type vegetative plant part;
(iii) an exogenous polynucleotide which encodes a plant PDAT polypeptide operably linked to a heterologous promoter which is effective to direct expression of the exogenous polynucleotide in the Sorghum species or Zea mays vegetative plant part and increase the level of the PDAT polypeptide relative to the corresponding wild type vegetative plant part, an another exogenous polynucleotide which encodes an RNA molecule which is complementary to a portion of an mRNA molecule encoding an endogenous TGD5 polypeptide and which down-regulates production of the endogenous TGD5 polypeptide in the Sorghum species or Zea mays vegetative plant part, wherein said another exogenous polynucleotide is operably linked to a heterologous promoter which is effective to direct expression of said another exogenous polynucleotide in the Sorghum species or Zea mays vegetative plant part and the RNA molecule down-regulates production of the endogenous TGD5 polypeptide relative to the corresponding wild type vegetative plant part, and an additional exogenous polynucleotide which encodes an RNA molecule which is complementary to a portion of an mRNA molecule encoding an endogenous SDP1 TAG lipase polypeptide and which down-regulates production of the endogenous SDP1 TAG lipase polypeptide in the Sorghum species or Zea mays vegetative plant part, wherein said additional exogenous polynucleotide is operably linked to a heterologous promoter which is effective to direct expression of said additional exogenous polynucleotide in the Sorghum species or Zea mays vegetative plant part and the RNA molecule down-regulates production of the endogenous SDP1 TAG lipase polypeptide relative to the corresponding wild type vegetative plant part;
and
(iv) an exogenous polynucleotide which encodes an RNA molecule which is complementary to a portion of an mRNA molecule encoding an endogenous SDP1 TAG lipase polypeptide and which down-regulates production of the endogenous SDP1 TAG lipase in the Sorghum species or Zea mays vegetative plant part, wherein the exogenous polynucleotide is operably linked to a heterologous promoter which is effective to direct expression of the exogenous polynucleotide in the Sorghum species or Zea mays vegetative plant part and the RNA molecule down-regulates production of the endogenous SDP1 TAG lipase polypeptide relative to the corresponding wild type vegetative plant part, an another exogenous polynucleotide which encodes an RNA molecule which is complementary to a portion of an mRNA molecule encoding an endogenous TGD5 polypeptide and which down-regulates production of the endogenous TGD5 polypeptide in the Sorghum species or Zea mays vegetative plant part, wherein said another exogenous polynucleotide is operably linked to a heterologous promoter which is effective to direct expression of said another exogenous polynucleotide in the Sorghum species or Zea mays vegetative plant part and the RNA molecule down-regulates production of the endogenous TGD5 polypeptide relative to the corresponding wild type vegetative plant part, and an additional exogenous polynucleotide which encodes an RNA molecule which is complementary to a portion of an mRNA molecule encoding an endogenous TST1 polypeptide and which down-regulates production of the endogenous TST1 polypeptide in the Sorghum species or Zea mays vegetative plant part wherein said additional exogenous polynucleotide is operably linked to a heterologous promoter which is effective to direct expression of said additional exogenous polynucleotide in the Sorghum species or Zea mays vegetative plant part and the RNA molecule down-regulates production of the endogenous TST1 polypeptide relative to the corresponding wild type vegetative plant part.