Patent ID: 11953436
Assignee: PROTEINSIMPLE
Field: Measurement (Instruments)
Classification: CPC G | IPC A  B  C  G

Claim 18:
19. A method, comprising:
applying, to a sample containing a plurality of protein isoforms, a fluorescent detection binder configured to bind to free thiols without reducing protein isoforms;
separating the sample to generate a set of separated protein isoforms;
obtaining a fluorescence signal and an absorbance signal associated with the set of separated protein isoforms;
identifying, based on the absorbance signal, at least one of a quantity or an identity of each separated protein isoform from the set of separated protein isoforms; and
measuring, based on the fluorescence signal, a quantity of free thiols associated with each separated protein isoform from the set of separated protein isoforms.