Patent ID: 11957715
Assignee: MILTENYI BIOTEC B.V. &CO.KG
Field: Pharmaceuticals (Chemistry)
Classification: CPC C  A | IPC A  C

Claim 0:
1. A method for transducing human NK cells with a nucleic acid, the method comprising:
a) obtaining a cell population that comprises NK cells,
b) activating a plurality of NK cells in the population comprising contacting the NK cells with one or a combination of cytokines, including interleukin-2 (IL-2) and/or interleukin-15 (IL-15), and then
c) contacting the activated NK cells with a plurality of pseudotyped retroviral vectors each containing said nucleic acid,
wherein the pseudotyped retroviral vectors comprise either:
(1) a chimeric envelope glycoprotein that comprises a fusion of the transmembrane and extracellular domain of a baboon endogenous retrovirus (BaEV) envelope glycoprotein with the cytoplasmic tail domain of a murine leukemia virus (MLV) envelope glycoprotein; or
(2) a BaEV envelope glycoprotein that has been recombinantly modified from wild type BaEV envelope glycoprotein by removing the glycoprotein's fusion inhibitory R peptide;
wherein contacting said activated NK cells with said vectors transduces said nucleic acid into the activated NK cells.