Patent ID: 11883508
Assignee: ONCOCLEW CO., LTD.
Field: Measurement (Instruments)
Classification: CPC A  C  G | IPC A  C

Claim 0:
1. A method for preparing a patient-derived lung cancer organoid xenograft animal model, comprising the steps of:
(a) culturing lung cancer cells, which have been obtained by cellizing lung cancer tissue isolated from a lung cancer patient, with a cell culture substrate, to obtain a lung cancer organoid;
(b) subjecting the obtained organoid to treatment with an enzyme and then allowing reaction to proceed;
(c) adding, to the lung cancer organoid after step (b), a cell culture substrate, and performing culture so that a 3-dimensional lung cancer organoid is formed;
(d) removing the cell culture substrate from the 3-dimensional lung cancer organoid;
(e) encapsulating the 3-dimensional lung cancer organoid, from which the cell culture substrate has been removed, in a membrane and then solidifying the same; and
(f) transplanting, into a subject animal, the 3-dimensional lung cancer organoid obtained in step (e), wherein the transplanting into a subject animal does not comprise injection of the 3-dimensional lung cancer organoid by syringe.