Patent ID: 11891439
Assignee: ASTUTE MEDICAL, INC.
Field: Measurement (Instruments)
Classification: CPC C  G | IPC C  G

Claim 12:
13. The method of claim 11, wherein
(a) the first monoclonal antibody or antigen binding fragment thereof is conjugated to a signal development element;
(b) the first monoclonal antibody or antigen binding fragment thereof forms a reaction mixture with the biological sample, and the biological sample is applied to the disposable test device by applying the reaction mixture to the disposable test device;
(c) the second monoclonal antibody or antigen binding fragment thereof is immobilized at the predetermined zone of a solid support;
(d) each of the first and second monoclonal antibodies or antigen binding fragments thereof is a rabbit, mouse, chicken, goat, sheep, donkey, human, llama or camelid antibody or antigen binding fragment thereof;
(e) at least one of the first and second monoclonal antibodies or antigen binding fragment thereof is a rabbit antibody or antibody fragment;
one or both of the first or second monoclonal antibodies or antigen binding fragment thereof is an F(ab) or F(ab′)2 fragment;
(g) the second monoclonal antibody or antigen binding fragment thereof forms a sandwich complex with CCL14 and the first monoclonal antibody or antigen binding fragment thereof; or
(h) the first and second monoclonal antibodies or antigen binding fragments thereof are a pair selected from the group consisting of a 10H3/10K1-2H3/2K1 pair, and a 10H3/10K1-31H1/31K1 pair, or in each case a corresponding antigen binding fragment thereof,
wherein 2H3/2K1 comprises three CDRs of a heavy chain variable region comprising the amino acid sequence of SEQ ID NO: 1, and three CDRs of a light chain variable region comprising the amino acid sequence of SEQ ID NO: 2, and
wherein 31H1/31K1 comprises three CDRs of a heavy chain variable region comprising the amino acid sequence of SEQ ID NO: 19, and three CDRs of a light chain variable region comprising the amino acid sequence of SEQ ID NO: 20.