Patent ID: 11891626
Assignee: DISCGENICS, INC.
Field: Pharmaceuticals (Chemistry)
Classification: CPC C  A | IPC A  C

Claim 0:
1. A discogenic cell population comprising:
cells derived from mammalian nucleus pulposus disc tissue, wherein the cell population has been passaged at least one time in an anchorage dependent culture, and transferred and maintained in-vitro in anchorage independent culture comprising methylcellulose at a concentration of about 0.1% to about 5% or a low adhesion coating, wherein the cell population, after seven days in anchorage independent culture, expresses at least 2-fold more aggrecan gene and collagen 2 gene than a population of nucleus pulposus cells derived from mammalian disc tissue grown in anchorage dependent culture, and wherein less than about 40% of the cell population expresses the cell surface markers CD24 and CD105.