Patent ID: 11867706
Assignee: LABORATORY CORPORATION OF AMERICA HOLDINGS
Field: Measurement (Instruments)
Classification: CPC G | IPC G

Claim 14:
15. A computer-program product tangibly embodied in a non-transitory machine-readable storage medium, including instructions configured to cause one or more computers to perform actions to identify a subject as a CBD user or a THC user by measuring relative levels of at least one THC metabolite and at least one CBD metabolite in a urine sample comprising the following steps:
(a) performing tandem MS/MS on the urine sample to obtain MS/MS data comprising a predetermined MS/MS transition for the at least one THC metabolite and the at least one CBD metabolite, wherein the predetermined MS/MS transition comprises a transition for at least one predefined precursor ion of defined m/z and at least one quantitative fragment ion of a defined m/z for each of the at least one THC metabolite and the at least one CBD metabolite, and wherein the at least one THC metabolite comprises 11-carboxy Δ9-THC (COO-THC) and THC released from Δ9-THC glucuronide, and the at least one CBD metabolite comprises 7-carboxy-CBD (COO-CBD) and CBD released from CBD glucuronide;
(b) determining an amount of the at least one THC metabolite and the at least one CBD metabolite in the urine sample based on the transitions in step (a);
(c) determining a ratio of the amount of the at least one CBD metabolite to the amount of the at least one THC metabolite for the urine sample; and
(d) identifying the urine sample as being from a subject who has ingested THC and/or CBD and/or is indeterminate for ingesting either THC and/or CBD based on the ratio of the amount of the at least one CBD metabolite to the amount of the at least one THC metabolite for the urine sample.