Patent ID: 11891454
Assignee: ICHNOS SCIENCES SA
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 13:
14. An in vitro method for the production of the hetero-dimeric immunoglobulin or fragment thereof of claim 1 comprising the following steps:
ia) preparing a DNA vector encoding a heavy chain of the first polypeptide and a DNA vector encoding a heavy chain of the second polypeptide wherein one or both DNA vectors or a third DNA vector optionally encode a common light chain or a light chain that assembles with a heavy chain of the first or second polypeptide; or
ib) preparing one DNA vector encoding heavy chains of the first and second polypeptides wherein the DNA vector optionally encodes a common light chain or a light chain that assembles with a heavy chain of the first or second polypeptide; and
wherein said DNA vectors are suitable for transient or stable expression in a mammalian host cell;
ii) transfecting or co-transfecting the DNA vector(s) from (ia) or (ib) in a mammalian host cell line;
iii) culturing the transfected cell line or stably selected clone therefrom and harvesting the cell culture supernatant;
iv) contacting the cell culture supernatant on a Protein A affinity chromatography resin; and
v) eluting and collecting the hetero-dimeric immunoglobulin of interest.