Patent ID: 11898179
Assignee: PRESIDENT AND FELLOWS OF HARVARD COLLEGE
Field: Pharmaceuticals (Chemistry)
Classification: CPC C  A | IPC A  C

Claim 24:
25. A method of editing a polynucleotide encoding an ion channel in a DRG neuron, the method comprising contacting the ion channel-encoding polynucleotide with:
(i) a fusion protein comprising: (a) a guide nucleotide sequence-programmable DNA binding protein domain; and (b) a cytosine deaminase domain; and
(ii) a guide nucleic acid molecule targeting the fusion protein of (i) to a target C base in the ion channel-encoding polynucleotide;
whereby the contacting results in deamination of the target C base by the fusion protein, resulting in a C to T change in the ion channel-encoding polynucleotide, and
wherein the guide nucleic acid molecule is selected from SEQ ID NOs: 339-1456.