Patent ID: 11900573
Assignee: CARL ZEISS MICROSCOPY GMBH
Field: Computer technology (Electrical engineering)
Classification: CPC G | IPC G

Claim 0:
1. A method for generating an overview contrast image of a sample carrier in a microscope, which images a sample arranged on the sample carrier, in which
a) the sample carrier is illuminated in transmitted light from a two-dimensional illumination array, which has individually switchable single light sources,
b) multiple different raw overview images of the sample carrier are recorded,
wherein:
each of the raw overview images is generated in that precisely one of the single light sources is switched on, wherein an individual one of the single light sources is switched on for each raw overview image, so that N raw overview images are recorded,
c) intermediate images are generated from the raw overview images, in that
c1) brightness ranks are defined for pixels of the raw overview images,
c2) each intermediate image is assigned to precisely one brightness rank, and
c3) in each intermediate image, for each pixel of this intermediate image, the raw overview image is searched out in the raw overview images which has the brightness rank at the corresponding pixel which is assigned to the respective intermediate image, and the brightness value of the corresponding pixel of the found raw overview image is used, and

d) the intermediate images are combined to form the overview contrast image, wherein the intermediate image assigned to the first brightness rank is not taken into consideration and is not incorporated into the overview contrast image.