Patent ID: 11885818
Assignee: ROCHE DIAGNOSTICS OPERATIONS, INC.
Field: Measurement (Instruments)
Classification: CPC C  G | IPC C  G

Claim 6:
7. A method for the mass spectrometric determination of an analyte molecule comprising the steps:
(a) reacting the analyte molecule with a compound of formula A as defined in claim 1, whereby a covalent adduct of the analyte molecule and the compound of formula A is formed, and
(b) subjecting the adduct from step (a) to a mass spectrometric analysis,
the mass spectrometric analysis comprising:
(i) subjecting an ion of the adduct to a first stage of mass spectrometric analysis, whereby the ion of the adduct is characterised according to its mass/charge (m/z) ratio,
(ii) causing fragmentation of the adduct ion, whereby a first neutral entity, is released and a daughter ion of the adduct is generated, wherein the daughter ion of the adduct differs in its m/z ratio from the adduct ion, and
(iii) subjecting the daughter ion of the adduct to a second stage of mass spectrometric analysis, whereby the daughter ion of the adduct is characterized according to its m/z ratio, and/or
wherein (ii) may further comprise alternative fragmentation of the adduct ion, whereby a second neutral entity different from the first neutral entity is released and a second daughter ion of the adduct is generated, and
wherein (iii) may further comprise subjecting the first and second daughter ions of the adduct to a second stage of mass spectrometric analysis, whereby the first and second daughter ions of the adduct are characterised according to their m/z ratios.