Patent ID: 11859207
Assignee: THE CATHOLIC UNIVERSITY OF KOREA INDUSTRY-ACADEMIC COOPERATION FOUNDATION
Field: Pharmaceuticals (Chemistry)
Classification: CPC A  C | IPC A  C

Claim 0:
1. A method to make one or more antigen-presenting cells, comprising:
a) eliminating HLA-A, HLA-B and HLA-C in one or more 293T cells using a CRISPR-Cas9 system to make one or more HLA-null 293T cells,
wherein the CRISPR-Cas9 system comprises single guide RNAs(sgRNAs) targeting each of:
HLA-A exon 2(SEQ ID NO: 1) and exon 3(SEQ ID NO: 2),
HLA-B exon 2(SEQ ID NO: 3) and exon 3(SEQ ID NO: 4),
HLA-C exon 2(SEQ ID NO: 5) and exon 3(SEQ ID NO: 6); and

b) introducing a co-stimulatory molecule group into the one or more HLA-null 293T cells to make one or more antigen-presenting cells,
wherein the co-stimulatory molecule is selected from the group consisting of: CD80; CD83; CD54; CD32; 4-1BBL; and CD70.