Patent ID: 11913054
Assignee: BASF SE
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 1:
2. A process for fermentative production of n-BA comprising the steps of:
I) growing in a fermenter a recombinant microorganism comprising a nucleic acid molecule encoding an AAT gene encoding an AAT enzyme having an n-butylacrylate (n-BA) forming activity, wherein the microorganism also comprises a butanol producing pathway and an acryloyl-CoA producing pathway, and wherein the microorganism has an introduced, increased, or enhanced n-butylacrylate forming activity and/or expression of said AAT enzyme;
wherein the AAT gene encoding an AAT enzyme having an n-BA forming activity is selected from the group consisting of:
(I) a nucleic acid molecule comprising a sequence selected from the group consisting of SEQ ID NO: 1, 3, 5, 7, 9, 11 and 13;
(II) a nucleic acid molecule having at least 95% identity to a nucleic acid molecule selected from the group consisting of SEQ ID NO: 1, 3, 5, 7, 9, 11 and 13;
(III) a nucleic acid molecule hybridizing to the full complement of a nucleic acid molecule having a sequence selected from the group consisting of SEQ ID NO: 1, 5, 7, 9, 11 and 13;
(IV) a nucleic acid molecule encoding a polypeptide selected from the group consisting of SEQ ID NO: 2, 6, 8, 10, 12, and 14; and
(V) a nucleic acid molecule encoding a polypeptide having at least 95% identity to a polypeptide selected from the group consisting of SEQ ID NO: 2, 6, 8, 10, 12, and 14; and
II) recovering n-BA from a fermentation broth obtained in step I).