Patent ID: 11859217
Assignee: DNA SCRIPT
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 5:
6. The TdT variant of claim 1 comprising the following combination of substitutions with respect to SEQ ID NO: 3:
(i) A17V+Q37E+D41R+L52F+G57E+M63R+S94R+G98E+A108V+S119A+L131R+S 146E+Q149R+C173G+R207L+K265T+G284P+E289V+R325P+Q326F+E328N+H337D+R351K+W377R;
(ii) A17V+Q37E+D41R+L52F+G57E+M63R+S94R+G98E+A108V+S146E+Q149R+C173G+F193Y+V199M+M201V+R207L+K265T+G284P+E289V+R325A+Q326F+E328N+R351K;
(iii) L52F+A108V+R351K+A17V+Q37E+D41R+G57E+C59R+L60D+M63R+S94R+G98E+S119A+L131R+5146E+Q149R+C173G+R207L+K265T+G284P+E289V+R325A+Q326F+E328N;
(iv) L52F+A108V+R351K+A17V+Q37E+D41R+G57E+C59R+L60D+M63R+S94R+G98E+K118Q+S119A+L131R+S146E+Q149R+C173G+R207L+K265T+G284P+E289V+R325A+Q326F+E328N+W377R;
(v) A17V+Q37E+D41R+L52F+G57E+C59R+L60D+M63R+S94R+G98E+A108V+S119A+L131R+5146E+Q149R+C173G+R207L+F259S+Q261L, G284P+E289V+R325A+Q326F+E328N+R351K+W377R;
(vi) A17V+Q37E+D41R+L52F+G57E+C59R+L60D+M63R+S94R+G98E+A108V+S119A+L131R+S146E+Q149R+C173G+R207L+E257D+F259S+K260R+Q261L+G284P+E289V+R325A+Q326F+E328N+R351K+W377R;
(vii) A17V+D41R+L53F+G57E+C59R+L60D+M63R+S94R+G98E+K118Q+S119A+L131R+S146E+Q149R+C173G+R207L+K265T+G284P+E289V+R325A+Q326F+R351K+W377R;
(viii) A17V+D41R+L52F+G57E+C59R+L60D+M63R+S94R+G98E+A108V+S119A+L131R+S146E+Q149R+R207L+K265T+G284P+E289V+R325A+Q326F+R351K;
(ix) A17V+L52F+M63R+A108V+C173G+R207L+K265T+G284P+E289V+R325P+E328N+R351K;
(x) A17V+D41R+L52F+G57E+M63R+S94R+G98E+A108V+S146E+Q149R+C173G+M184T+R207L+K209H+G284L+E289A+R325V+E328K+R351K;
(xi) A17V+L52F+G57E+M63R+A108V+C173G+R207L+K265T+G284P+E289V+R325P+E328N+R351K; or
(xii) A17V+L32T+Q37R+D41R+L52F+G57E+C59R+L60D+M63R+S67A+S94R+G98E+A108V+S119A+L131R+5146E+Q149R+V171A+5172E+C173R+V182I+5183E+R207L+K209H+M210K+T211I+E223G+A224P+E228D+Q261L+G284P+E289V+R325A+Q326F+E328N+R351K+D372E.