Patent ID: 11913946
Assignee: INDUSTRY-UNIVERSITY COOPERATION FOUNDATION HANYANG UNIVERSITY
Field: Measurement (Instruments)
Classification: CPC G  A | IPC A  G

Claim 12:
13. A method for detecting a target material based on surface-enhanced Raman scattering (SERS), comprising:
a) preparing a sample solution comprising a target material to be detected;
b) immobilizing a first antibody for the target material onto a magnetic nanoparticle;
c) immobilizing a second antibody for the target material onto the metal nanoprobe according to claim 5;
d) forming an immune complex wherein the target material and the first antibody of the magnetic nanoparticle are conjugated by adding the first antibody-immobilized magnetic nanoparticle of b) to the sample solution of a);
e) forming a sandwich immune complex of the second antibody of the metal nanoprobe, the target material and the first antibody of the magnetic nanoparticle by adding the second antibody-immobilized metal nanoprobe of c) to the solution comprising the first antibody-conjugated immune complex of d);
f) separating the magnetic nanoparticle and the metal nanoprobe not forming the sandwich immune complex using a magnetic field; and
g) measuring a Raman signal of the sandwich immune complex.