Patent ID: 11958886
Assignee: UNIVERSITY OF FLORIDA RESEARCH FOUNDATION, INC.
Field: Pharmaceuticals (Chemistry)
Classification: CPC C  A | IPC A  C

Claim 0:
1. A method of treating osteoarthritis in a subject, the method comprising:
intra-articularly administering a pharmaceutical composition comprising:
(a) a recombinant adeno-associated virus (rAAV) particle comprising a codon-modified gene encoding interleukin-1 receptor antagonist (IL-1Ra), and
(b) a pharmaceutically acceptable carrier,
wherein the codon-modified gene is selected from the group consisting of:
(i) a codon-modified gene encoding human IL-1Ra and having a nucleotide sequence mismatch of 10-30% compared to the wild-type human IL-1Ra gene as set forth in SEQ ID NO: 9; and
(ii) a codon-modified gene encoding equine IL-1Ra and having a nucleotide sequence mismatch of 10-30% compared to the wild-type equine IL-1Ra gene as set forth in SEQ ID NO: 1;

wherein the codon-modified gene increases expression of IL-1Ra compared to a wild-type gene encoding IL-1Ra, and
wherein the administering is to a joint of the subject to treat osteoarthritis.