Patent ID: 11965199
Assignee: SANOFI
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 19:
20. A kit comprising:
(a) the cell of claim 18;
(b) a multicistronic expression vector of claim 19; or
(c) (i) an expression vector comprising a nucleic acid encoding for a CYP21A2 protein or a functional variant thereof;
(ii) one or more expression vectors comprising one or more nucleic acids encoding for at least one heterologous electron transfer system capable of transferring electrons to CYP21A2, wherein the at least one electron transfer system is selected from the group consisting of:
the combination of a flavodoxin reductase (Fpr) and an adrenotoxin;
the combination of a Fpr and the ferredoxin domain of an electron transfer domain (etpfd);
the combination of an adrenotoxin reductase homolog (Arh) and an adrenotoxin; and

the combination of an Arh and etpfd; and optionally
(iii) one or more expression vectors comprising one or more nucleic acids encoding for chaperones facilitating folding of CYP21A2.