Patent ID: 11951171
Assignee: TIANJIN MEDICAL UNIVERSITY
Field: Pharmaceuticals (Chemistry)
Classification: CPC A  C | IPC A  C

Claim 17:
18. The method for purifying extracellular vesicle according to claim 17, comprising step 2, combining the polypeptide EXP labeled with His with 100 μg CP05 and 40 μl nickel bead in 200 μl binding buffer and incubating for 1 h at 4° C.; step 3, coating a nickel bead with the polypeptide EXP labeled with His, adding a His-CP05 to 1 ml pre-centrifuged serum and incubating at 4° C. for 30 min; step 4, discarding the serum and washing off any nonspecifically bound molecules with a washing solution three times for 10 min each; and step 5, utilizing 100 μl eluent to extract the extracellular vesicle.