Patent ID: 11946911
Assignee: SHIMADZU CORPORATION
Field: Measurement (Instruments)
Classification: CPC G | IPC G

Claim 15:
16. The method according to claim 10, wherein, the step S2 further comprises:
step S21′ of separating a component of the sample to be analyzed by using supercritical chromatography and transporting the weakly polar substance to the first reverse phase liquid chromatography column of the reverse phase liquid chromatography part;
step S22′ of removing the supercritical mobile phase flowing out of the supercritical chromatography part in the first reverse phase liquid chromatography column; and
step S23′ of introducing into the first reverse phase liquid chromatography column, a mobile phase for transporting the weakly polar substance present in the first reverse phase liquid chromatography column to the second reverse phase liquid chromatography column to obtain a vitamin D as the component,
wherein transport of the weakly polar substance to the first reverse phase liquid chromatography column in the step S21′ is performed at the same time as the step S22′.