Patent ID: 11885732
Assignee: VISIONGATE, INC.
Field: Measurement (Instruments)
Classification: CPC G  A | IPC G

Claim 11:
12. A method for morphometric genotyping of cells in fluid flow in a capillary tube using optical tomography, the method comprising:
a) applying pressure to a plurality of embedded cells until at least one of the plurality of embedded cells appears in a field of view of an optical tomography viewing subsystem;
b) operating the optical tomography system to acquire a plurality of pseudo-projection images of the at least one embedded cell that is in the field of view by rotating the capillary tube about a tube axis to generate a plurality of pseudo-projection images at different views;
c) repeating operations a) and b) to provide a set of pseudo-projection images for each embedded cell;
d) reconstructing each embedded cell using data from the set of pseudo-projection images to produce a set of 3D cell image reconstructions;
e) segmenting each 3D image of the set of 3D cell image reconstructions to separate a cell image from background;
f) further segmenting the cell image to separate a nucleus from the cell image;
g) computing a plurality of morphological features characteristic of mutation drivers from each cell image;
h) operating a biological specimen classifier to determine a feature value from the plurality of morphological features characteristic of mutation drivers, wherein the biological specimen classifier is the classifier generated by the supervised learning of claim 1; and
i) classifying the 3D cell image into a cell type category by comparing the feature value to a predetermined threshold value.