Patent ID: 11891641
Assignee: LALLEMAND USA, INC.
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 0:
1. A recombinant yeast host cell for making an increased amount of a heterologous protein, wherein the recombinant yeast host cell:
(i) (a) has a first heterologous nucleic acid encoding the heterologous protein; and
(b) expresses a variant CYR1 protein; and/or

(ii) is obtained by introducing the first heterologous nucleic acid encoding the heterologous protein in an ancestral yeast host cell that expresses a variant CYR1 protein;
wherein the variant CYR1 protein:
provides to the recombinant yeast host cell or the ancestral yeast host cell a similar intracellular cAMP production in the presence and in the absence of a cAMP stimulus known to stimulate intracellular cAMP production in a control yeast cell, and
comprises the amino acid sequence of SEQ ID NO: 8 with a substitution at position 869 or a variant thereof having 90% identity with the amino acid sequence of SEQ ID NO: 8 with a substitution at position 869.