Patent ID: 11879898
Assignee: DH TECHNOLOGIES DEVELOPMENT PTE. LTD.
Field: Measurement (Instruments)
Classification: CPC G | IPC G

Claim 0:
1. A method for analyzing total testosterone in a sample comprising the steps:
creating a sample solution by combining:
a tagging reagent reactive with testosterone,
an aqueous precipitation agent that precipitates proteins from solution,
an internal standard solution containing a known concentration of an isotopically enriched testosterone, and
a sample containing or suspected to contain sample testosterone,

mixing the sample solution to precipitate proteins and react any testosterone present with the tagging reagent to form a mixture of a precipitate and a reacted liquid solution,
separating the precipitate from the reacted liquid solution,
analyzing the reacted liquid solution using tandem mass spectrometry to identify and/or quantitate the sample testosterone.