Patent ID: 11856944
Assignee: TRANSMEDICS, INC.
Field: Basic materials chemistry (Chemistry)
Classification: CPC A | IPC A

Claim 15:
16. A method of preserving a donor lung comprising:
flushing the donor lung antegrade prior to excising the donor lung from a donor's body with a first flushing solution;
flushing the donor lung retrograde prior to excising the donor lung from a donor's body with a second flushing solution;
excising the donor lung from the donor's body;
placing the donor lung on an organ care system (OCS);
perfusing the donor lung in a lung perfusion circuit of the OCS with a perfusion solution including at least one nutrient, at least one colloid, at least one buffer, and magnesium anhydrate to maintain the donor lung ex-vivo at a near physiological temperature and in a near physiologic functioning condition and to permit normal cellular metabolism in the donor lung, wherein the perfusion solution is supplemented with a circulating hematocrit of between 15% to 30%;
wherein the first flushing solution and the second flushing solution each include at least one nutrient, at least one colloid, at least one buffer, and magnesium sulfate anhydrate; and
wherein the first flushing solution comprises nitroglycerin at a concentration higher than a concentration of nitroglycerin in the second flushing solution.