Patent ID: 11952636
Assignee: VISBY MEDICAL, INC.
Field: Measurement (Instruments)
Classification: CPC C  G | IPC C

Claim 16:
17. A probe set for detecting presence and susceptibility of a target organism to a drug, comprising:
a first oligonucleotide probe substantially complementary to a non-polymorphic locus in a polynucleotide of the target organism;
a second oligonucleotide probe substantially complementary to a polymorphic locus in a polynucleotide of the target organism, the second probe comprising a nucleotide sequence matched to an allele of the polymorphic locus associated with resistance to the drug; and
a third oligonucleotide probe substantially complementary to the polymorphic locus, the third probe comprising a nucleotide sequence matched to an allele of the polymorphic locus associated with susceptibility to the drug, wherein
the second oligonucleotide probe has a second probe melting temperature for hybridization to the polymorphic locus comprising the allele of the polymorphic locus associated with resistance to the drug,
the third oligonucleotide probe has a third probe melting temperature for hybridization to the polymorphic locus comprising the allele of the polymorphic locus associated with susceptibility to the drug, and
the second oligonucleotide probe melting temperature is different from the third oligonucleotide probe melting temperature.