Patent ID: 11932874
Assignee: NATURECELL CO., LTD.
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 0:
1. A method for culturing mesenchymal stem cells in a culture medium, comprising the steps of (a)-(c) or (a′)-(c′):
(a) preparing vitamin C pre-treated gamma-sterilized fetal bovine serum (FBS) by irradiating gamma rays to FBS pre-treated with 0.1-1% of vitamin C;
(b) culturing mesenchymal stem cells for 2 days in a stem cell culture medium containing the vitamin C pre-treated gamma-sterilized FBS; and
(c) further culturing in a stem cell culture medium containing gamma-irradiated FBS and 0.1-1% of vitamin C or a stem cell culture medium containing the vitamin C pre-treated gamma-sterilized FBS; or
(a′) preparing vitamin C post-treated gamma-sterilized FBS by treating 0.1-1% of vitamin C to gamma-irradiated FBS;
(b′) culturing the mesenchymal stem cells for 2 days in a stem cell culture medium containing the vitamin C post-treated gamma-sterilized FBS; and
(c′) further culturing in a culture medium containing gamma-irradiated FBS and 0.1-1% vitamin C;
wherein the stem cell culture medium is a mixture of KSFM-P(Keratinocyte Serum Free Medium-P) medium and DMEM(Dulbecco's Modified Eagle's Medium) medium; and
wherein the cell number, viability, and doubling time of the cultured mesenchymal stem cells are at the same level as mesenchymal stem cells that have been cultured by medium supplemented with gamma-irradiated FBS for 2 days, and thereafter cultured by medium supplemented with non-irradiated FBS.