Patent ID: 11970786
Assignee: BIOO SCIENTIFIC CORPORATION
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 0:
1. A method for determining a nucleic acid contamination in a sample, the method comprising:
forming a reaction composition in a partition, the reaction composition comprising:
at least one unique combination of a spike in control and corresponding at least one spike in control primer, the at least one spike in control primer comprising a synthetic, unique nucleotide barcode,
at least one nucleic acid adapter, and
the sample, the sample comprising a plurality of sample nucleic acids;

fragmenting the plurality of sample nucleic acids to generate sample nucleic acid fragments;
ligating the at least one nucleic acid adapter to the sample nucleic acid fragments to generate ligation products;
amplifying the reaction composition using the at least one spike in control primer to generate a multiplicity of amplification products comprising a multiplicity of ligation product amplicons and a multiplicity of spike in control amplicons, each spike in control amplicon comprising a nucleotide tag that is unique to the spike in control amplicons generated using the corresponding at least one spike in control primer;
sequencing the multiplicity of ligation product amplicons and the multiplicity of spike in control amplicons to generate sequencing data;
detecting nucleic acid contamination in the sample, the contamination comprising an amplified spike in control not associated with the reaction composition, if the sequencing data exceeds a predetermined rejection parameter value indicative of one or more contaminating nucleic acids; and
rejecting the sample if nucleic acid contamination in the sample is determined,
wherein a concentration of the at least one spike in control in the reaction composition is between 0.1% to 2.0% (weight/weight %) of the plurality of sample nucleic acids in the reaction composition.