Patent ID: 11859243
Assignee: SEEGENE, INC.
Field: Computer technology (Electrical engineering)
Classification: CPC C  G | IPC C  G

Claim 3:
4. The method of claim 1, wherein (i) RVT1(D1D2) is obtained by (i-1) incubating the first target nucleic acid sequence with the first signal-generating means for detection of the first target nucleic acid sequence, (i-2) detecting signals at the first detection temperature and the second detection temperature, and (i-3) then obtaining a difference between the signals detected at the first detection temperature and the second detection temperature, and (ii) RVT2(D1D2) is obtained by (ii-1) incubating the second target nucleic acid sequence with the second signal-generating means for detection of the second target nucleic acid sequence, (ii-2) detecting signals at the first detection temperature and the second detection temperature, and (ii-3) then obtaining a difference between the signals detected at the first detection temperature and the second detection temperature; wherein RVT1(D1D2) is different from RVT2(D1D2).