Patent ID: 11912734
Assignee: THE JOHNS HOPKINS UNIVERSITY
Field: Organic fine chemistry (Chemistry)
Classification: CPC C | IPC C

Claim 23:
24. A method for synthesizing an oligonucleotide, the method comprising:
(a) providing a nucleoside phosphoramidite comprising a 2′-deoxyribose moiety having a 3′-hydroxyl position and a 5′-hydroxyl position, wherein the nucleoside phosphoramidite has a dimethoxytrityl protecting group at the 3′-hydroxyl position and a phosphoramidite moiety at the 5′-hydroxyl position, wherein the nucleoside phosphoramidite has the following structure:, and
(b) contacting the nucleoside phosphoramidite of (a) with one or more nucleoside residues attached to a solid phase support to form a solid support-bound product, wherein the synthesized oligonucleotide is selected from the group consisting of:

5′-d(T10×T4) SEQ ID NO: 1   17

5′-d(TTT AGG CGT GGT GAT GCT GTG TGC TAT GGT) SEQ ID NO: 2   18

5′-d(GCT GAT GCG X) SEQ ID NO: 3   19

5′-d(CGC AXC GCT GCG) SEQ ID NO: 4   20

5′-d(GTG CXT GTT TGT) SEQ ID NO: 5   21

5′-d(AAC CXG AGG CCC) SEQ ID NO: 6   22

5′-d(AAC CGG AXG CCC) SEQ ID NO: 7   23

5′-d(GGA AGC AAT XGT ACG G) SEQ ID NO: 8   24

5′-d(CCG ACX TCG CAT CAG C) SEQ ID NO: 13   25

5′-d(AGG GCG GTG TXG GAA GAG GGA) SEQ ID NO: 9   26

5′-d(AAC CXG AGG CCC ATC CTC AC) SEQ ID NO: 10   27

5′-d(GTG CXT GTT TGT GCC TGT CC) SEQ ID NO: 11   28

5′-d(TGT TCA TCA TGG GTC XTC GGT ATA TCC CAT) SEQ ID NO: 12   29

x=Fapy·dG.