Patent ID: 11898161
Assignee: RIKEN
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC A  C

Claim 0:
1. A method of improving the differentiation efficacy of human pluripotent stem cells to ectodermal cells, which comprises culturing human pluripotent stem cells, which are dissociated single cells, in a serum-free culture medium which is suitable for induction of differentiation of pluripotent stem cells into ectodermal cells by a Serum-free Floating culture of Embryoid Body-like aggregates (SFEB) method,
wherein the culture medium comprises a Rho kinase (ROCK) inhibitor for at least 2 days,
wherein, during culturing, induction of differentiation occurs, and
wherein the ectodermal cells are neural cells, or neural precursor cells.