Patent ID: 11873530
Assignee: 10X GENOMICS, INC.
Field: Biotechnology (Chemistry)
Classification: CPC C  G | IPC C

Claim 0:
1. A method for analyzing a cellular metabolite, comprising:
(a) providing: (i) a nucleic acid barcode molecule comprising a barcode sequence and a capture sequence; (ii) a cellular metabolite; and (iii) a riboswitch capable of binding to the cellular metabolite, wherein the riboswitch comprises a nucleic acid sequence comprising a riboswitch capture sequence and a riboswitch identifier sequence, wherein the riboswitch capture sequence is capable of coupling to the capture sequence of the nucleic acid barcode molecule, wherein the riboswitch capture sequence is inaccessible to the capture sequence of the nucleic acid barcode molecule in an absence of the cellular metabolite bound to the riboswitch, and wherein the riboswitch capture sequence is accessible to the capture sequence of the nucleic acid barcode molecule when the cellular metabolite is bound to the riboswitch;
(b) binding the riboswitch to the cellular metabolite, thereby rendering the riboswitch capture sequence accessible to the capture sequence of the nucleic acid barcode molecule;
(c) coupling the riboswitch capture sequence to the capture sequence of the nucleic acid barcode molecule; and
(d) with the riboswitch capture sequence coupled to the capture sequence, using the riboswitch and the nucleic acid barcode molecule to synthesize a nucleic acid molecule comprising (1) a first nucleic acid sequence corresponding to the riboswitch identifier sequence, and (2) a second nucleic acid sequence corresponding to the barcode sequence.