Patent ID: 11867685
Assignee: CHEMCOM S.A.
Field: Measurement (Instruments)
Classification: CPC G | IPC G

Claim 0:
1. A method for identifying an agent or a sample comprising one or more agent(s) that interfere with the binding between an OR5A2 receptor and musk compounds, said method comprising:
a) contacting a chimeric receptor with said agent or sample, wherein said chimeric receptor comprises a central region of the OR5A2 receptor, encompassing the transmembrane domains 2 to 7 having the amino acid sequence of SEQ ID NO: 11, or a polypeptide sequence having at least 95% sequence identity to SEQ ID NO: 11, with the proviso that said polypeptide has a proline at position 114 of said polypeptide sequence having at least 95% sequence identity to SEQ ID NO: 11 and,
wherein the central region is fused at its N-terminus to the N-terminal extracellular moiety, the transmembrane domain 1 and the intracellular loop 1 of a G protein-coupled receptor, and which is fused at its C-terminus to the intracellular C-terminal end of a G protein-coupled receptor,
wherein the chimeric receptor is capable of binding musk compounds selected from the group consisting of: nitromusks, macrocyclic musks, polycyclic musks, and linear musks;
b) measuring a signaling activity of the chimeric receptor in the presence of said agent or sample; and
c) comparing the activity measured in the presence of said agent or sample to the activity measured in a reaction in which the chimeric receptor is contacted with one or more musk compound(s) at its/their EC50, wherein said agent or sample is identified as an agent or a sample, that modulates the activity of said chimeric receptor as defined herein when the amount of the activity measured in the presence of the agent or sample is at least 10% of the amount induced by said musk compound(s) at its/their EC50.