Patent ID: 11873531
Assignee: ALCEDIAG
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 14:
15. The method according to claim 1, wherein ADARs specific isoforms are determined in the method, and wherein the pair of primers specific for the human ADAR mRNA PCR amplification are selected from the group consisting of:
(A) for ADAR1-150 isoform mRNA amplification:
Forward: 5′-GCCTCGCGGGCGCAATGAATCC-3′ (SEQ ID NO. 14),
Reverse: 5′-CTTGCCCTTCTTTGCCAGGGAG-3′ (SEQ ID NO. 15);
(B) for ADAR1-110 isoform mRNA amplification:
Forward: 5′-CGAGCCATCATGGAGATGCCCTCC-3′ (SEQ ID NO. 16),
Reverse: 5′-CATAGCTGCATCCTGCTTGGCCAC-3′ (SEQ ID NO. 17); and
(C) for ADAR2 mRNA amplification:
Forward: 5′-GCTGCGCAGTCTGCCCTGGCCGC-3′ (SEQ ID NO. 18),
Reverse: 5′-GTCATGACGACTCCAGCCAGCAC-3′ (SEQ ID NO. 19).