Patent ID: 11906301
Assignee: SCREEN HOLDINGS CO., LTD.
Field: Measurement (Instruments)
Classification: CPC G | IPC G

Claim 0:
1. A thickness evaluation method of a cell sheet, comprising:
capturing a plurality of tomographic images of the cell sheet using optical coherence tomography by causing light to be incident on the cell sheet in a in a direction intersecting a principal surface of the cell sheet; and
obtaining a thickness distribution of the cell sheet based on a result of the tomography imaging,
wherein the imaging step includes:
scanning repeatedly the light in a main scanning direction while moving an incident position of the light in a sub-scanning direction intersecting the main scanning direction at a feed pitch larger than a size corresponding to one pixel in the tomographic images; and
capturing a tomographic image of the cell sheet at each scan in the main scanning direction, thereby generating the plurality of the tomographic images corresponding to cross-sections at positions different from each other in the sub-scanning direction,

wherein the obtaining step includes:
obtaining one-dimensional thickness distributions of the cell sheet in the corresponding cross-sections based on each of the plurality of tomographic images; and
obtaining a two-dimensional thickness distribution of the cell sheet by interpolating the one-dimensional thickness distributions, and

wherein the method further comprises:
capturing a plurality of partial tomographic images corresponding to a partial region of the cell sheet while moving the light incident position in the sub-scanning direction at a standard interval determined in advance,
obtaining the one-dimensional thickness distributions of the partial region in cross-sections corresponding to the partial tomographic images, and
setting the feed pitch larger than the standard pitch based on the obtained one-dimensional thickness distributions.