Patent ID: 11884972
Assignee: CARLSBERG A/S
Field: Biotechnology (Chemistry)
Classification: CPC C  A | IPC A  C

Claim 12:
13. The method according to claim 12, comprising:
Following step c), obtaining a fraction of each gDNA sample from each sub-pool; and
Combining a plurality of fractions into super-pools, thereby obtaining gDNA sample super-pools comprising gDNA samples from a plurality of sub-pools, wherein gDNA from each sub-pool is only present in one super-pool; and, wherein steps d) and e) comprise:
Performing a plurality of PCR amplifications, each amplification comprising (i) a gDNA sample super-pool, (ii) one or more sets of primers, each set flanking a target sequence, and (iii) PCR reagents; thereby amplifying one or more target sequences, wherein each PCR amplification comprises a plurality of compartmentalised PCR amplifications, each comprising part of said gDNA sample super-pool; and
Detecting PCR amplification products comprising one or more target sequences comprising the one or more predetermined mutations in the one or more NOIs, thereby identifying one or more super-pools that comprise said one or more predetermined mutations.