Patent ID: 11890396
Assignee: ADVANCED SOLUTIONS LIFE SCIENCES, LLC
Field: Medical technology (Instruments)
Classification: CPC A  B | IPC A  B

Claim 17:
18. A method of treating a segmental bone defect in a patient, the method comprising:
providing a bone graft comprising a 3D-printed graft core formed of a pre-bone constituent comprising demineralized bone matrix (DBM) and a carrier, and a pre-vascularized shell at least partially enrobing the graft core, the pre-vascularized shell formed of a pre-vascularized shell constituent comprising from about 80,000 to about 500,000 microvessels/ml isolated intact microvessel fragments, mesenchymal stem cells, and from about 1.5 to about 7 mg/ml collagen; and
placing the bone graft in a segmental bone defect region of the patient; wherein the microvessel fragments inosculate and the bone graft progresses to native, mature bone in the patient.