Patent ID: 11899023
Assignee: REGENERON PHARMACEUTICALS, INC.
Field: Measurement (Instruments)
Classification: CPC G | IPC G

Claim 0:
1. A method for identifying at least one dimer species, said method comprising:
contacting a sample including the dimer species to a chromatographic system having a size-exclusion chromatography resin;
washing said size-exclusion chromatography resin using a mobile phase to provide an eluent including the dimer species;
identifying the dimer species in said eluent using an electrospray ionization mass spectrometer under native conditions, wherein at least one splitter with at least three paths is used to allow fluid communication between an ultraviolet detector to the chromatographic system having the size-exclusion chromatography resin; and
wherein the eluent from washing the size-exclusion chromatography resin is introduced in the ultraviolet detector through the at least one splitter at a flow rate of about 0.2 mL/min to about 0.4 mL/min.