Patent ID: 11879139
Assignee: PORTON ADVANCED SOLUTIONS LTD.
Field: Chemical engineering (Chemistry)
Classification: CPC C  B | IPC C

Claim 20:
21. A method of purifying a population of recombinant lentivirus particles, said method comprising:
obtaining a sample comprising the recombinant lentivirus particles and a contaminant;
subjecting the sample to a multimodal chromatography column comprising a multimodal chromatographic medium which retains the contamiant;
collecting a flow-through liquid from the multimodal chromatography column, wherein the flow-through liquid comprises the recombinant lentivirus particles;
subjecting the flow-through liquid to a SXC column comprising a hydrophilic medium in the presence of a PEG rendering the recombinant lentivirus particles bound to the hydrophilic medium; and
eluting the SXC column with an elution buffer to obtain a preparation of the recombinant lentivirus particles,
wherein the method does not comprise a step which retains the recombinant lentivirus particles to an ion exchange chromatography column.