Patent ID: 11862296
Assignee: NATIONAL KAOHSIUNG UNIVERSITY OF SCIENCE AND TECHNOLOGY
Field: Computer technology (Electrical engineering)
Classification: CPC G | IPC G

Claim 4:
5. The method for designing the multi-objective primer pair as claimed in claim 1, wherein the at least two objectives include the fourth objective confirming whether nucleotide types in 3′ ends of both the forward primer and the reverse primer are ‘G’ or ‘C’, and if yes, a GC clamp of the primer pair formed by the forward primer and the reverse primer is equal to 0;
confirming whether nucleotide types in the 3′ ends of either the forward primer or the reverse primer are ‘G’ or ‘C’, and if yes, the CG clamp is equal to 1;
confirming whether nucleotide types in the 3′ ends of the forward primer and the reverse primer are neither ‘G’ nor ‘C’, and if yes, the GC clamp is equal to 2;
calculating a length difference between the forward primer and 10 the reverse primer and taking an absolute value thereof to obtain a first length;
setting an optimal length of the forward primer and an optimal length of the reverse primer and calculating a difference between the optimal length of the forward primer and the optimal length of the reverse primer and taking an absolute value thereof to obtain a second length, wherein if the first length is not greater than the second length, a length difference is equal to 0, and if the first length is greater than the second length, the length difference is equal to a difference between the first length and the second length, and the fourth objective is equal to a sum of the GC clamp and the length difference.