Patent ID: 11919002
Assignee: 10X GENOMICS, INC.
Field: Chemical engineering (Chemistry)
Classification: CPC B | IPC B

Claim 2:
3. A method for producing droplets of a first liquid in a second liquid, the method comprising steps (a) to (d) in the following order:
a) providing a device comprising:
i) a first channel having a first depth, a first width, a first proximal end, and a first distal end;
ii) a droplet formation region in fluid communication with the first channel;
iii) a collection reservoir in fluid communication with the droplet formation region and configured to collect droplets formed in the droplet formation region, wherein the first channel and droplet formation region are configured to produce first droplets of the first liquid in the second liquid, wherein the first droplets comprise a sample; and
iv) a droplet or particle source in fluid communication with the collection reservoir, wherein the droplet or particle source does not comprise the sample;
b) allowing the first liquid to flow from the first channel to the droplet formation region to produce the first droplets;
c) collecting the first droplets in the collection reservoir; and
d) allowing the droplet or particle source to provide second droplets and/or particles to the collection reservoir, wherein each second droplet and/or particle does not comprise the sample, and wherein the particles are solid or semi-solid.