Patent ID: 11866703
Assignee: LANZHOU VETERINARY RESEARCH INSTITUTE, CHINESE ACADEMY OF AGRICULTURAL SCIENCES
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 4:
5. The knockout method according to claim 2, further comprising constructing the pEtNMT::DHFR plasmid before step a, comprising:
i. ligating upstream of a 5′UTR of an EtNMT gene, DHFR-TS*, and downstream of a 3′UTR of the EtNMT gene into a pcDNA3.1 (+) vector to obtain a ligated vector;
ii. transforming the ligated vector into competent cells; and
iii. extracting a resulting plasmid to obtain the pEtNMT::DHFR plasmid.