Patent ID: 11913056
Assignee: SHENZHEN BLUEPHA BIOSCIENCES CO., LTD.
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 2:
3. The method according to claim 2, wherein the method further comprises one or more of the following steps:
(1) expressing a polyhydroxyalkanoate polymerase variant capable of synthesizing poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) wherein the polyhydroxyalkanoate polymerase variant comprises the amino acid sequence of SEQ ID NO: 29; and
(2) enhancing expression and/or enzyme activity of (R)-enoyl-CoA hydratase, the enhanced expression and/or enzyme activity of (R)-enoyl-CoA hydratase is achieved by initiating the transcription of a gene encoding (R)-enoyl-CoA hydratase in the Ralstonia eutropha genome with a promoter comprising the nucleotide sequence of SEQ ID NO: 30.