Patent ID: 11971336
Assignee: SHIMADZU CORPORATION
Field: Measurement (Instruments)
Classification: CPC G  C  H | IPC C  G  H

Claim 14:
15. A method for preparing a sample comprising a glycan, the method comprising:
performing a lactonization reaction in which at least a part of sialic acids included in the glycan is lactonized; and
performing an amidation reaction in which lactones of lactonized sialic acids are amidated through addition of an amidation reaction solution to the sample, wherein the amidation reaction solution comprises ammonia, an amine, or a salt thereof is reacted with the lactonized sialic acids to open lactone rings and amidate lactones of the lactonized sialic acids, and wherein:
the amine is a primary amine;
pH of the amidation reaction solution is 7.7 or higher; and
in the lactonization reaction, at least one selected from the group consisting of α2,3-sialic acid, α2,8-sialic acid, and α2,9-sialic acid among the sialic acids is lactonized, wherein the amidation reaction solution does not include a dehydration condensation agent that is reacted with the lactones, and wherein an operation that reacts the sample with a dehydration condensation agent is not performed after the addition of the amidation reaction solution to the sample.