Patent ID: 11918957
Assignee: DONALDSON COMPANY, INC.
Field: Chemical engineering (Chemistry)
Classification: CPC B  C | IPC B  C

Claim 14:
15. A method for preparing adsorptive media, the method comprising:
contacting a macroporous support with a first solution comprising a coupling agent and a first solvent to form a macroporous support having coupling groups; and
contacting the macroporous support having coupling groups with an incubating solution comprising water, an organic solvent, and a molecule having an affinity to a biological target to couple the ligand with at least some of the coupling groups,
wherein the organic solvent is present in the incubating solution in an amount ranging from 75% to 125% of amount of organic solvent at a cloud point of the ligand, and
wherein the molecule comprises a nucleotide, an oligonucleotide, a peptide, a polypeptide, a protein, or any combination thereof.