Patent ID: 11919006
Assignee: INDIAN INSTITUTE OF TECHNOLOGY, KHARAGPUR
Field: Measurement (Instruments)
Classification: CPC B  G  C | IPC B  C  G

Claim 5:
6. The method, as claimed in claim 1, wherein said microcontroller controlled isothermal heating unit operations following RT LAMP reactions is carried out for SARS-CoV-2 and influenza A detection from direct swab/saliva and wherein said method comprises carrying out the microcontroller based isothermal heating operations by ramping up and ramping down of temperature of the heating block by performing: a) for the initial RT LAMP reaction: (i) ramping-up of the heating block from room temperature to a targeted temperature of 62-68° C. for 4-5 minutes; (ii) placing the enclosed reaction microchamber hosting the RT LAMP reaction mixture into the unit and continuing said microcontroller controlled piecewise isothermal heating operations for the next 25 to 35 minutes whereby the pathogenic RNA gets converted into cDNA and subsequently gets amplified; and (iii) automatically further ramping up of the heating block after completion of the amplification process at said temperature of 62-68° C., to reach the temperature of 93-98° C. and continuing termination process of 3-8 minutes at 95° C.; and (b) for subsequent hybridization: automatically ramping down of the heating block temperature to 48-55° C. after the termination process is over, to facilitate the hybridization process occurring with the 6-fluorescein amidite and di-deoxy nucleotide labelled DNA oligonucleotide probe specifically binding to the amplified 5′-biotin labelled target DNA and generating said dual labelled-RT LAMP-based hybridized reaction products.