Patent ID: 11946054
Assignee: PRECIGEN, INC.
Field: Pharmaceuticals (Chemistry)
Classification: CPC C  A  G | IPC C  G

Claim 0:
1. A method for integrating heterologous gene(s) into the genome of a peripheral blood mononuclear cell (PMBC), the method comprising delivering to the cell:
(a) a non-naturally occurring polynucleotide comprising (i) an AttP or AttB recombination sequence and (ii) the heterologous gene(s); and
(b) a polynucleotide encoding a SF370, SPβc2, Bxb1, A118, or φRv1 serine recombinase;, wherein the heterologous gene(s) encode: (i) a chimeric antigen receptor; and (ii) a cell tag comprising an amino acid sequence having at least 90% identity with any one of SEQ ID NOs: 190-195 and 197-200.