Patent ID: 11965002
Assignee: THE BD OF TRUSTEES OF THE UNIVERSITY OF ILLINOIS
Field: Pharmaceuticals (Chemistry)
Classification: CPC C  A | IPC A  C

Claim 18:
19. A method of reversibly and allosterically regulating the activity of a non-receptor tyrosine phosphatase comprising:
inserting into the β10 sheet of the catalytic domain of the non-receptor tyrosine phosphatase a light-responsive construct comprising a first photoreceptive polypeptide domain and a second photoreceptive polypeptide domain, wherein said first and second photoreceptive polypeptide domains:
(i) are operatively-linked via a flexible linker;
(ii) independently comprise
(a) amino acid residues 37-184 of SEQ ID NO:1,
(b) amino acid residues 40-186 of SEQ ID NO:1,
(c) a variant of (a) or (b) comprising one or more amino acid substitutions selected from the group consisting of Ile74Val, Ile85Val, Met135Ile, and Met165Ile, or
(d) a variant of amino acids 37-186 of SEQ ID NO:1 comprising one or more amino acid substitutions selected from the group consisting of Ile74Val, Ile85Val, Met135Ile, and Met165Ile; and

(iii) are capable of dimerizing with each other thereby producing a fusion protein; and

exposing the fusion protein to an effective dose of a light to modulate a conformation of the fusion protein, wherein the modulation of the conformation of the fusion protein regulates the activity of the non-receptor tyrosine phosphatase.