Patent ID: 11921045
Assignee: NATIONAL UNIVERSITY CORPORATION KOBE UNIVERSITY
Field: Measurement (Instruments)
Classification: CPC G  C | IPC C  G

Claim 11:
12. A holographic three-dimensional multi-spot light stimulator method comprising:
irradiating a plurality of stimulation objects with fluorescence excitation light;
acquiring hologram information of a three-dimensional fluorescence distribution using a self-interfering fluorescence signal light of the stimulation objects;
reconstructing the acquired hologram information of the three-dimensional fluorescence distribution with a computer, thereby facilitating observation of a state of at least one stimulation object;
generating a hologram for light stimulation that simultaneously forms a plurality of light spots at the positions of a plurality of stimulation target objects identified with the same or substantially the same spatial resolution and three-dimensional observation range as the acquired hologram of the three-dimensional fluorescence distribution;
controlling a spatial light modulator with respect to the pattern of the hologram for light stimulation;
spatially forming a plurality of light spots using the hologram for light stimulation and simultaneously applying light stimulation to a plurality of the stimulation target objects; and
after applying light stimulation to the plurality of the stimulation target objects, reconstructing the acquired hologram information of the three-dimensional fluorescence distribution with a computer, thereby facilitating observation of a state of at least one stimulation object after application of light stimulation.