Patent ID: 11939630
Assignee: SHAANXI LIFEGEN CO., LTD.
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 0:
1. A specific primer probe combination for HLA-B*15:02 allele, comprising:
HLA-B*15:02 allele-specific forward primer Fm, the nucleotide sequence of Fm is set forth in SEQ ID NO: 1;
non-HLA-B*15:02 allele-specific forward primer Fn1, the nucleotide sequence of Fn1 is set forth in SEQ ID NO: 2;
non-HLA-B*15:02 allele-specific forward primer Fn2, the nucleotide sequence of Fn2 is set forth in SEQ ID NO: 3;
reverse primer R, the nucleotide sequence of R is set forth in SEQ ID NO: 4; and
probe P: 5′-Carboxy-X-Rhodamine-ACGCAGCCTGGGACCCTGTGTGCCAGCA quenching group-3′, the nucleotide sequence between Carboxy-X-Rhodamine and the quenching group is set forth in SEQ ID NO: 5;, wherein Carboxy-X-Rhodamine is a fluorescent group, and the quenching group has a chemical structure of,