Patent ID: 11932846
Assignee: TECHNOLOGY INNOVATION MOMENTUM FUND (ISRAEL) LIMITED PARTNERSHIP
Field: Measurement (Instruments)
Classification: CPC C  G | IPC C

Claim 0:
1. A construct system comprising:
(i) a first nucleic acid construct comprising a first polynucleotide having a nucleic acid sequence that encodes a N-terminal fragment of chloramphenicol acetyl transferase (CAT), said N-terminal fragment comprising a first portion of the catalytic active site of said CAT, said N-terminal fragment being devoid of acetylating activity; and
(ii) a second nucleic acid construct comprising a second polynucleotide having a nucleic acid sequence that encodes a C terminal fragment of said CAT, said C-terminal fragment comprising a second portion of the catalytic active site of said CAT, said C-terminal fragment being devoid of acetylating activity; and
wherein said N-terminal fragment is capable of associating with said C-terminal fragment to generate an active CAT that is capable of acetylating chloramphenicol,
wherein said N terminal fragment consists of the amino acid sequence as set forth in SEQ ID NO: 2 or 6; and/or
said C-terminal fragment consists of the amino acid sequence as set forth in SEQ ID NOs: 3 or 7.