Patent ID: 11946162
Assignee: MASSACHUSETTS INSTITUTE OF TECHNOLOGY
Field: Computer technology (Electrical engineering)
Classification: CPC C  G | IPC C  G

Claim 0:
1. A method for scarless stitching comprising
identifying at least three consecutive units of a genetic construct, wherein each of the at least three consecutive units is in a partial plasmid and is flanked by a 4-10 nucleotide upstream and downstream flanking sequence that forms 5′ cohesive ends when digested with a restriction enzyme, wherein the at least three consecutive units comprises a first unit, a second unit, and a third unit, wherein the first unit, the second unit, and the third unit each comprise one or more nif cluster genetic components,
digesting the first and second units with a first restriction enzyme and digesting the third unit with a second restriction enzyme to produce cohesive ends, and
ligating the cohesive ends to produce a plasmid.