Patent ID: 11884935
Assignee: KOREA INSTITUTE OF SCIENCE AND TECHNOLOGY
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 7:
8. A composition for inducing trans-differentiation of a fibroblast into a cardiomyocyte comprising first exosomes isolated from stem cells during a mesoderm induction process, and second exosomes isolated during a cardiac specification and maturation process in the differentiation of stem cells into cardiomyocytes, and a chemical agent for inducing cardiomyocyte differentiation, wherein the chemical agent is a mixture of CHIR99021 and PD0325901;
wherein the first exosomes comprise miR-291a-3p, miR-292a-3p, miR-292a-5p, miR-293-3p, miR-293-5p, miR-295-3p, miR-294-3p, miR-292b-5p, miR-148a-3p, miR-363-3p, miR429-3p, miR291a-5p, miR-200b-3p, miR-672-5p, miR-335-3p, miR-302b-3p, miR-200a-5p, miR-881-3p, and miR-135b-5p, and the expression of miR-291a-3p, miR-292a-3p and miR-292a-5p is more than twice that of the fibroblast;
wherein the second exosomes comprise miR-1a-3p, miR-302d-3p, miR-292a-3p, miR-291a-ep, miR-302c-3p, miR-335-3p, miR-431-5p, miR-376b-3p, miR-434-3p, miR-1b-5p, miR-434-3p, miR-293-3p, miR-291a-5p, miR-302b-3p, miR-126a-5p, miR-355-5p, miR-126b-3p, miR-148a-3p, miR-292b-3p, miR-292a-5p and miR-363-3p, and the expression of miR-1a-3p and miR-302d-3p is more than twice that of the fibroblast;
wherein the fibroblast is isolated from the same species as the stem cells; and
wherein a mixing ratio of the first exosomes and the second exosomes is from 1:19 to 19:1.