Patent ID: 11929532
Assignee: BIOCHEMINSIGHTS, INC.
Field: Surface technology, coating (Chemistry)
Classification: CPC H  C  Y | IPC C  H

Claim 0:
1. A device comprising:
a) an anode contained in an anode chamber and a cathode contained in a cathode chamber, the two chambers being separated by at least one membrane which allows water molecules, either neutral or positively charged, to pass through the at least one membrane from one chamber to the other chamber, wherein the anode chamber consists of deionized water therein in contact with the anode and optionally oxygen and proton;
b) an aqueous medium containing a biological system in the cathode chamber in contact with the cathode, said biological system being capable of performing a reductive biological process;
c) a loop for continuously recirculating the aqueous medium from an external vessel through the cathode chamber and back in to the external vessel;
d) an external power source providing a voltage between the anode and the cathode;
e) a detection instrumentation having a continuous fluorometric detector for monitoring intracellular NADH or NADPH so as to control a degree of reduction in the reductive biological process; wherein the device further comprises a computer-controlled peristaltic pump for adjusting a flow rate of the aqueous medium at a level above a transitional flow rate where a relationship between the flow rate and current is non-linear; and
f) one or more separation membranes situated between the cathode and the biological system such that the cathode is not in direct contact with the biological system.