Patent ID: 11959927
Assignee: AMPRION, INC.
Field: Measurement (Instruments)
Classification: CPC G | IPC G

Claim 14:
15. The method of claim 14, wherein:
(i) the monomeric α-syn protein is present in a concentration of from 10 μM±10% to 30 μM±10%;
(ii) the buffer composition comprises PIPES and has a pH of from 6.2±10% to 6.5±10%;
(iii) the salt comprises NaCl in a concentration of from 500±10% mM to 700 mM±10%;
(iv) the borosilicate glass bead has a diameter of 2.45 mm±10%;
(v) the fluorescent protein aggregation indicator comprises thioflavin T (ThT) in a concentration of from 5 μM to 10 μM; and
(vi) the detection comprises measuring ThT fluorescence.