Patent ID: 11965184
Assignee: SIGMA-ALDRICH CO. LLC
Field: Biotechnology (Chemistry)
Classification: CPC C  Y | IPC C

Claim 2:
3. The fusion protein of claim 1, further comprising an optional second linker, wherein the nuclear localization signal, the marker protein, the first linker, the optional second linker (if present), and the Cas9 protein are arranged in the following order (N-terminus to C-terminus):
marker protein-first linker-nuclear localization signal-Cas9 protein;
marker protein-nuclear localization signal-first linker-Cas9 protein;
nuclear localization signal-marker protein-first linker-Cas9 protein;
marker protein-first linker-nuclear localization signal-first linker-Cas9 protein; or
nuclear localization signal-second linker-marker protein-first linker-Cas9 protein.