Patent ID: 11923045
Assignee: TRIPLEBAR BIO, INC.
Field: Computer technology (Electrical engineering)
Classification: CPC G  C | IPC C  G

Claim 0:
1. A method comprising:
(a) subjecting a first plurality of partitions comprising proteins encoded by a first library of different genetic variants to different conditions to generate
a first dataset comprising data on each protein encoded by the first library of different genetic variants in each of the different conditions;
(b) based on the first dataset, identifying one or more of the first library of different genetic variants encoding a first protein that is improved in at least one performance criteria;
(c) generating a second plurality of partitions comprising proteins encoded by a second library of different genetic variants, the proteins encoded by the second library of genetic variants having at least 70% homology to the first protein that is improved in the at least one performance criteria;
(d) subjecting the second plurality of partitions comprising proteins encoded by the second library of different genetic variants to the different conditions to generate a second dataset comprising data on each protein encoded by the second library of different genetic variants in each of the different conditions;
(e) based on the second dataset, identifying one or more of the second library of different genetic variants encoding a second protein that is improved in the at least one performance criteria compared to the first protein that is improved in the at least one performance criteria;
(f) generating a third plurality of partitions comprising proteins encoded by a third library of different genetic variants, the proteins encoded by the third library of different genetic variants having at least 70% homology to the second protein that is improved in the at least one performance criteria;
(g) subjecting the third plurality of partitions comprising proteins encoded by the third library of different genetic variants to the different conditions to generate a third dataset comprising data on each protein encoded by the third plurality of different genetic variants in each of the different conditions;
(h) using a feedback controller to (i) maintain a frequency of generation of the second plurality of partitions or third plurality of partitions or (ii) regulate a threshold gate used to select a target percentage of the first plurality of partitions in (a), the second plurality of partitions in (d), or third plurality of partitions in (g); and
(i) based on the third dataset, identifying one or more of the third library of different genetic variants encoding a third protein that is improved in the at least one performance criteria compared to the second protein that is improved in the at least one performance criteria.