Patent ID: 11965179
Assignee: nan
Field: Pharmaceuticals (Chemistry)
Classification: CPC C  A | IPC A  C

Claim 5:
6. A method of creating a pooled platelet lysate product, the method comprising:
obtaining whole blood from a source:
separating the platelets from the whole blood comprises using a pressurized rotating microfluidic filtration system, the pressurized rotating microfluidic filtration system including an outer tube surrounding an inner tube and rotating relative to the inner tube, wherein the inner tube is porous and is configured to prevent transfer of larger sized molecules into the outer tube, thus separating the platelets from the whole blood,
creating platelet rich plasma (PRP) by concentrating the platelets in plasma and removing red blood cells and white blood cells using centrifugation;
centrifuging the PRP, removing a portion of a volume of the plasma, and re-suspending the PRP in the remaining plasma to create concentrated PRP (C-PRP); laser activating the C-PRP to create an activated C-PRP product; freezing and thawing the activated C-PRP product at least 3 times, wherein vortexing is performed after each thaw; and reconstituting the activated C-PRP in a saline to create a platelet lysate product.