Patent ID: 11898167
Assignee: THE J. DAVID GLADSTONE INSTITUTES, A TESTAMENTARY TRUST ESTABLISHED UNDER THE WILL OF J. DAVID GLADSTONE
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 0:
1. A device for confining mammalian cells, forcing cellular/tissue alignment and tissue self-assembly, comprising:
a cell adhesion substrate; and
a removable elastomeric stencil overlay;
wherein the elastomeric stencil has one or more cut-out patterned microwells comprising two or more circular, oval, rectangular, square, V-shaped, or triangular holes, each hole joined to an adjacent hole by a canal (shaft); and
wherein the cell adhesion substrate binds cells within the holes and the canals of the cut-out pattern; and
wherein at least one of the microwells has at least two square holes with a side length “L” of 250 μm to 1000 μm, and the canal joining the two square holes has a longitudinal length “X” of 250-1000 μm and a traverse width “Y” of 50-200 μm.