Patent ID: 11969480
Assignee: TRANSLATE BIO, INC.
Field: Pharmaceuticals (Chemistry)
Classification: CPC A  B  C | IPC A  B  C

Claim 2:
3. A process of encapsulating messenger RNA (mRNA) in preformed empty lipid nanoparticles (LNPs), wherein the lipid component of the LNPs comprises or consists of a cationic lipid, a non-cationic lipid, a PEG-modified lipid, and optionally cholesterol and said process comprises the following steps:
adding a first volume of a suspension comprising the preformed empty LNPs to a solution comprising the mRNA such that a mixture comprising LNPs encapsulating the mRNA is formed;
adding one or more additional volumes of the suspension comprising the preformed empty LNPs to the mixture obtained in the preceding step until a desired molar ratio of cationic lipid to mRNA is achieved; and
wherein the first volume and the one or more additional volumes of the suspension comprising the preformed empty LNPs are added continuously without intervening periods of heating or mixing.