Patent ID: 11913060
Assignee: EDENIQ, INC.
Field: Measurement (Instruments)
Classification: CPC C  G | IPC C  G

Claim 0:
1. A method of accurately quantifying a concentration of mixed-linkage beta-glucan in a plant biomass sample, the method comprising:
contacting the plant biomass sample with an alkaline mixture thereby generating a pretreated sample, wherein the alkaline mixture comprises sodium hydroxide or potassium hydroxide at a concentration between 0.2 N and 1 N;
wherein the contacting is at a pretreatment temperature between 10° C. and 35° C. and a pretreatment duration between 20 minutes and less than 3 hours;
hydrolyzing mixed-linkage beta-glucan in the pretreated sample to generate glucose;
measuring the concentration of glucose in the pretreated sample; and
transforming the measured glucose concentration to a calculated mixed-linkage beta-glucan concentration by multiplying the measured glucose concentration value by a conversion factor 1.11 g glucose/1 g mixed-linkage beta-glucan, wherein the calculated mixed-linkage beta-glucan concentration accurately quantifies the concentration of mixed-linkage beta-glucan in the plant biomass sample.