Patent ID: 11913049
Assignee: RAMON GONZALEZ
Field: Biotechnology (Chemistry)
Classification: CPC C  Y | IPC C

Claim 2:
3. The method of claim 1, wherein said enzymes catalyzing the sequence of reactions for the oxygen-independent activation and conversion to the acyl-CoA and generation of the product precursor acetyl-CoA and the acyl-CoA or the keto-acid comprises:
a. the alkyl succinate synthase, wherein the alkyl succinate synthase is overexpressed;
b. an overexpressed 2-methyl-alkyl-succinyl-CoA synthetase that catalyzes the conversion of said 2-methyl-alkyl-succinate to a 2-methyl-alkyl-succinyl-CoA;
c. an overexpressed 2-methyl-alkyl-succinyl-CoA dehydrogenase that catalyzes the conversion of said 2-methyl-alkyl-succinyl-CoA to 2-methyl-alkyl-2-butenoyl-CoA;
d. an overexpressed mesaconyl-C1-CoA-C4-CoA transferase that catalyzes the conversion of said 2-methyl-alkyl-2-butenoyl-CoA to 3-methyl-alkyl-2-butenoyl-CoA;
e. an overexpressed mesaconyl-C4-CoA hydratase that catalyzes the conversion of said 3-methyl-alkyl-2-butenoyl-CoA to 3-methyl-alkyl-3-hydroxy-succinyl-CoA; and
f. an overexpressed citramalyl-CoA lyase that catalyzes the conversion of said 3-methyl-alkyl-3-hydroxy-succinyl-CoA to the product precursor acetyl-CoA and the keto-acid.