Patent ID: 11952586
Assignee: HOFFMANN-LA ROCHE, INC.
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 19:
20. The method according to claim 15, wherein the co-cultivating of the one or more B-cells is with about 2,500 to about 7,500 EL-4 B5 cells, which have been irradiated with gamma radiation of a dose less than 3 Gy, wherein the feeder mix comprises about 0.06 ng/ml to about 0.2 ng/ml IL-1beta, about 0.06 ng/ml to about 0.2 ng/ml TNFalpha, about 1.5 ng/ml to about 5 ng/ml IL-2, about 0.3 ng/ml to about 1 ng/ml IL-10, about 0.3 ng/ml to about 1 ng/ml IL-6, and about 0.43 ng/ml-0.73 ng/ml PMA.