Patent ID: 11970695
Assignee: ILLUMINA CAMBRIDGE LIMITED
Field: Biotechnology (Chemistry)
Classification: CPC C  B | IPC B  C

Claim 0:
1. A method of generating a solid support, the method comprising immobilizing a plurality of transposome complexes to the solid support, said transposome complexes comprising a transposase bound to a first polynucleotide and a second polynucleotide, said first polynucleotide comprising:
i. a 3′ portion comprising a transposon end sequence, and
ii. a first tag comprising a first tag domain;
wherein said second polynucleotide comprises a complementary transposon end sequence;
wherein said transposome complexes are assembled in solution; and wherein said immobilizing comprises (1) providing the transposase, the first polynucleotide, the second polynucleotide, and ligase in solution, and (2) ligating said first polynucleotide to an immobilized polynucleotide coupled to said solid support,
wherein said immobilized polynucleotide is hybridized to a splint oligonucleotide.