Patent ID: 11953436
Assignee: PROTEINSIMPLE
Field: Measurement (Instruments)
Classification: CPC G | IPC A  B  C  G

Claim 0:
1. A method, comprising:
generating, from a sample containing a plurality of protein isoforms, a first sample portion and a second sample portion, at least some protein isoforms from the plurality of protein isoforms having one or more free thiol groups;
applying, to the first sample portion, a fluorescent detection binder configured to bind to free thiol groups to produce conjugated thiols, without reducing the plurality of protein isoforms included in the first sample portion;
separating the plurality of protein isoforms in the first sample portion to generate a first set of separated protein isoforms;
separating the plurality of protein isoforms in the second sample portion to generate a second set of separated protein isoforms;
detecting a first fluorescence signal associated with the conjugated thiols and an absorbance signal associated with the first set of separated protein isoforms;
identifying, based on the absorbance signal, at least one of a quantity or an identity of each separated protein isoform from the first set of separated protein isoforms;
detecting a second fluorescence signal associated with the second set of separated protein isoforms; and
measuring, based on a difference between the first fluorescence signal and the second fluorescence signal, a quantity of free thiol groups associated with each separated protein isoform from the first set of separated protein isoforms.