Patent ID: 11920188
Assignee: nan
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 0:
1. A method for detecting Glade variants in a Coronavirus disease 2019 virus (COVID-19) in a sample, comprising:
obtaining the sample;
harvesting viruses from the sample;
isolating a total RNA from the harvested viruses;
performing a combined reverse transcription and first amplification reaction on the total RNA using at least one first primer pair selective for all COVID-19 viruses to generate COVID-19 virus cDNA amplicons;
performing a second amplification using the COVID-19 virus cDNA amplicons as template and at least one fluorescent labeled second primer pair selective for a target nucleotide sequence in the COVID-19 virus cDNA to generate at least one fluorescent labeled COVID-19 virus amplicon;
hybridizing the fluorescent labeled COVID-19 virus amplicons to a plurality of nucleic acid probes, each having a sequence corresponding to a sequence determinant that discriminates among the Glade variants of the COVID-19 virus, said nucleic acid probes attached to a solid microarray support;
washing the microarray at least once; and
imaging the microarray to detect at least one fluorescent signal from the hybridized fluorescent labeled COVID-19 virus amplicons, thereby detecting the Glade variants of the COVID-19 virus in the sample.