Patent ID: 11933707
Assignee: CHEMOMETEC A/S
Field: Measurement (Instruments)
Classification: CPC G | IPC G

Claim 0:
1. A method for characterising one or more objects in a sample, the method comprising the steps of:
collecting transmitted, refracted, scattered, diffracted, or emitted light from the sample, wherein the light comprises fluorescent or luminescent light, thus forming collected light,
forming by said collected light on a sensor surface
at least a first image and a second image of the object(s), wherein for at least the first and the second image the collected light is modulated asymmetrically differently after the sample,
processing
the at least first and second images by performing at least one of:
comparing the first image with the second image for determining a position of focus of an image cytometer or of a microscope based on a translation of a depiction of the one or more objects in the first image and the second image, and
subtracting the first image from the second image for increasing the contrast at the edges of the objects;

wherein the one or more objects is a cell or a particle, element or region of a cell.