Patent ID: 11951155
Assignee: ZHONGSHI DUQING(SHANDONG) BIOTECH CO., LTD
Field: Pharmaceuticals (Chemistry)
Classification: CPC A | IPC A

Claim 7:
8. The method according to claim 5, wherein in step 6), the separation and purification by analytical liquid chromatography is conducted using a 10 μm C18 100A 4.6×250 mm chromatographic column, the predetermined flow rate is 15 mL/min, the trifluoroacetic acid aqueous solution has a volume concentration of 0.1%, and the trifluoroacetic acid acetonitrile solution has a volume concentration of 0.1%; wherein the chromatographic column is operated at a flow rate of 1.0 mL/min with an injection volume of 10 μL, and an absorbance wavelength of 220 nm.