Patent ID: 11918642
Assignee: ARIZONA BOARD OF REGENTS ON BEHALF OF ARIZONA STATE UNIVERSITY
Field: Pharmaceuticals (Chemistry)
Classification: CPC A  C | IPC A  C

Claim 17:
18. The method of claim 17, wherein
the geminivirus-based plant expression vector comprises a T-DNA region comprising:
a first nucleic acid sequence encoding Gag and a first promoter region upstream of the first nucleic acid sequence encoding Gag; and
a second nucleic acid sequence encoding a fragment of gp41 and a second promoter upstream of the second nucleic acid sequence encoding a fragment of gp41; and

the at least one replicating but highly attenuated vaccinia virus vector is selected from the group consisting of:
a first replicating but highly attenuated vaccinia virus vector comprising a third nucleic acid sequence encoding Gag,
a second replicating but highly attenuated vaccinia virus vector comprising a fourth nucleic acid sequence encoding a fragment of gp41, and
a third replicating but highly attenuated vaccinia virus vector comprising the third nucleic acid sequence encoding Gag and the fourth nucleic acid sequence encoding fragment of gp41.