Patent ID: 11913049
Assignee: RAMON GONZALEZ
Field: Biotechnology (Chemistry)
Classification: CPC C  Y | IPC C

Claim 3:
4. The method of claim 1, wherein said enzymes catalyzing the sequence of reactions for the oxygen-independent activation and conversion to the acyl-CoA and generation of the product precursor acetyl-CoA and the acyl-CoA comprises:
a. the alkyl succinate synthase, wherein the alkyl succinate synthase is overexpressed;
b. an overexpressed succinyl-CoA:2-methyl-alkyl-succinyl-CoA transferase or 2-methyl-alkyl-succinyl-CoA synthetase that catalyzes the conversion of said 2-methyl-alkyl-succinate to a 2-methyl-alkyl-succinyl-CoA;
c. an overexpressed 2-methyl-alkyl-succinyl-CoA dehydrogenase that catalyzes the conversion of said 2-methyl-alkyl-succinyl-CoA to 2-methyl-alkyl-2-butenoyl-CoA;
d. an overexpressed mesaconyl-CoA hydratase/β-methylmalyl-CoA dehydratase that catalyzes the conversion of said 2-methyl-alkyl-2-butenoyl-CoA to 3-hydroxy-2-methyl-alkyl-succinyl-CoA;
e. an overexpressed β-methylmalyl-CoA lyase that catalyzes the conversion of said 3-hydroxy-2-methyl-alkyl-succinyl-CoA to glyoxylate and the acyl-CoA;
f. an overexpressed glyoxylate carboligase that catalyzes the conversion of said glyoxylate to tartronate semialdehyde;
g. an overexpressed tartronate semialdehyde reductase that catalyzes the conversion of said tartronate semialdehyde to D-glycerate;
h. an overexpressed glycerate kinase that catalyzes the conversion of said D-glycerate to 3-phospho-D-glycerate;
i. glycolytic enzymes that catalyze the conversion of said 3-phospho-D-glycerate to pyruvate, wherein the glycolytic enzymes are selected from the group consisting of phosphoglycerate mutase, enolase, and pyruvate kinase; and
j. a pyruvate formate lyase or pyruvate dehydrogenase that catalyze the conversion of said pyruvate to the acetyl-CoA.