Patent ID: 11898183
Assignee: nan
Field: Pharmaceuticals (Chemistry)
Classification: CPC C  A  Y | IPC A  C

Claim 15:
16. A method for constructing the recombinant microbe as claimed in claim 1, said method comprising: (a) obtaining one or more recombinant vector, said recombinant vector encoding a repertoire of genes encoding phenyl alanine ammonia-lyase (PAL), Cinnamate-4-hydroxylate (C4H), 4-coumaroyl CoA-ligase (4CL), hydroxycinnamoyl-CoA quinate hydroxycinnamoyltransferase (HCT), p-coumaroyl quinate 3′-hydroxylase (C3H), caffeoyl CoA O-methyltransferase (CCoAOMT), bifunctional pinoresinol-lariciresinol reductase (DIRPLR), secoisolariciresinol dehydrogenase (SDH), O-methyltransferase (OMT), 2-oxoglutarate/Fe(II)-dependent dioxygenase (2-ODD), 2-Deoxy-d-ribose-5-phosphate aldolase, Cytochrome P450 oxidoreductase CYP719, Cytochrome P450 oxidoreductase CYP71, Cytochrome P450 oxidoreductase CYP82D, UDP glucosyl transferase, and at least one gene encoding a protein transporter selected from the group consisting of ATP-Binding Cassette (ABC) transporter, Major Facilitator Superfamily (MFS) transporters, SMR (small multidrug resistant) family, RND (Resistance-Nodulation-Cell Division) family, and the MATE (multidrug and toxic compound extrusion) family; and (b) transforming a host microbe with the recombinant vector obtained in step (a), to obtain the recombinant microbe as claimed in claim 1.