Patent ID: 11913046
Assignee: INBIOSE N.V.
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 11:
12. A yeast cell comprising:
a) at least one heterologous polynucleotide encoding a transporter protein that facilitates export of 2′ fucosyllactose from the yeast cell, wherein the transporter protein is selected from the group consisting of a Sugars Will Eventually be Exported Transporter (SWEET) family transporter, a Sugar Efflux Transporter A (SetA) family transporter, and a sugar porter family transporter;
b) at least one heterologous polynucleotide encoding a guanosine diphosphate (GDP)-mannose-4,6-dehydratase (EC 4.2.1.47);
c) at least one heterologous polynucleotide encoding a GDP-4-keto-6-D-deoxymannose epimerase-reductase (EC 1.1.1.271);
d) at least one heterologous polynucleotide encoding a 2-α-L-fucosyltransferase (EC 2.4.1.69);
wherein the yeast cell produces 2′ fucosyllactose, and

e) at least one polynucleotide encoding a lactose transporter having at least 90% identity to a peptide selected from the group consisting of SEQ ID NO: 24, 25, 26, 27, and 28.