Patent ID: 11858966
Assignee: THE UAB RESEARCH FOUNDATION
Field: Measurement (Instruments)
Classification: CPC C  G | IPC C  G

Claim 1:
2. A nucleic acid sequence encoding a mutant single-chain Mycobacterium smegmatis porin (Msp), wherein the nucleic acid sequence comprises:
(a) a first and second nucleotide sequence, wherein the first nucleotide sequence encodes a first Msp monomer sequence and the second nucleotide sequence encodes a second Msp monomer sequence; and
(b) a third, fourth, fifth, sixth, seventh, and eighth nucleotide sequence or any subset thereof, wherein the third, fourth, fifth, sixth, seventh, and eighth nucleotide sequences encode a third, fourth, fifth, sixth, seventh, and eighth Msp monomer sequence, respectively, wherein the first, second, third, fourth, fifth, sixth, seventh and eighth nucleotide sequence or any subset thereof are arranged consecutively in the nucleic acid; and
(c) at least one ninth nucleotide sequence encoding an amino acid linker sequence, wherein the ninth nucleotide sequence is positioned between any two consecutive nucleotide sequences encoding Msp monomer sequences, wherein at least one of the first and second Msp monomer sequences is the mutant Msp monomer sequence of claim 1.