Patent ID: 11926813
Assignee: MOLECULAR DEVICES, LLC
Field: Measurement (Instruments)
Classification: CPC C  G | IPC C  G

Claim 33:
34. The method of claim 1, wherein the step of determining a measure of likelihood that the candidate colony is monoclonal comprises
correlating the position of the candidate colony in a pick day image with the position of each of the plurality of single originator cells in a day zero volumetric image stack and cell movement downward drift over time comprising
determining a cell location probability cone for each of the single originator cells in the day zero volumetric image stack, wherein each cell location probability cone comprises an inverted cone with its apex at a starting position of one of the single originator cells;
determining a hemisphere defined by a selected radius centered on the candidate colony; and
observing how many cell location probability cones intersect the hemispherical volume defined by the selected radius centered on the candidate colony,
wherein the determining the measure of likelihood that the candidate colony is monoclonal comprises observing that no more than one originator cell was found in the first volumetric stack within the selected radius centered on the candidate colony.