Patent ID: 11919971
Assignee: PRESIDENT AND FELLOWS OF HARVARD COLLEGE
Field: Measurement (Instruments)
Classification: CPC C  G | IPC C  G

Claim 0:
1. A method of making a substrate having an entity attached thereto, the method comprising:

i) contacting the substrate with a CO2 plasma to form a modified substrate comprising a plasma-generated-moiety (PGM) comprising a carboxylic acid group, wherein the substrate comprises polysulfone (PS), polyarylethersulfone (PAES) or polyethersulfone (PES); and
ii) contacting the entity with the modified substrate under conditions sufficient for attachment of the entity to the modified substrate; wherein step ii) contacting is performed for about 4-16 hours;, thereby making a substrate having the entity attached thereto,
provided that
the entity comprises a lectin; and, provided that:
the modified substrate is not contacted with a crosslinking moiety of derivatized with a crosslinking moiety, prior to attachment of the entity;
the modified substrate is not contacted with an organic solvent prior to attachment of the entity; and
the entity is contacted with the modified substrate under aqueous conditions.