Patent ID: 11951233
Assignee: ACRO BIOMEDICAL COMPANY. LTD.
Field: Medical technology (Instruments)
Classification: CPC A  Y | IPC A

Claim 0:
1. A method of producing an acellular organ, comprising:
(a) subjecting an organ to a first supercritical fluid (SCF) in the presence of a first co-solvent under a first pressure of 200-500 bar at a first temperature between 30-50° C. for a first period of 10-100 minutes; and
(b) subjecting the first SCF treated organ of the step (a) with a continuous flow of a second SCF in the presence of a second co-solvent under a second pressure of 200-500 bar at a second temperature between 30-50° C. for a second period of 10-100 minutes, wherein the flow rate of the second SCF is 10-30 liter per minute;
wherein, the method does not comprise the step of treating the organ with an agent selected from the group consisting of an enzyme, an ion chelating agent, a detergent, a glycerol, and a combination thereof.