Patent ID: 11879160
Assignee: ANHUI AGRICULTURAL UNIVERSITY
Field: Biotechnology (Chemistry)
Classification: CPC C  Y | IPC C

Claim 0:
1. A method for identifying feed intake (FI), relative feed intake (RFI) and feed conversion of a duck, the method comprising:
a). extracting total DNA from the venous blood of duck wings;
b). conducting PCR amplification on the total extracted DNA using forward primer, SEQ ID NO: 2 and reverse primer, SEQ ID NO: 3;
c). subjecting the PCR product to polymerase chain reaction single strand conformation polymorphism (PCR-SSCP) and sequencing neuropeptide gene, NPY, comprising the nucleotide of SEQ ID NO 1 to determine both alleles at position 557 of SEQ ID NO 1 to obtain a genotype of the duck; and
d). determining the FI, RF1, and feed conversion based on the genotype of the duck determined in step c) wherein a CC genotype indicates a lower FI, lower RFI, and extremely high feed conversion than ducks with a CT and TT genotypes.