Patent ID: 11965205
Assignee: VEDABIO, INC.
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 13:
14. The method of claim 1, further comprising the steps of:
providing third ribonucleoprotein complexes (RNP1-NAs), wherein each of the RNP1-NAs comprises a third nucleic acid-guided nuclease and third gRNAs (gRNA1-NAs); wherein the gRNA1-NAs comprise a sequence complementary to a nucleic acid target of interest, and wherein the third nucleic acid-guided nuclease exhibits trans-cleavage activity;
contacting the reaction mix with the sample under conditions that allow nucleic acid targets of interest in the sample to bind the RNP1-NA, wherein:
upon binding of the nucleic acid target to one of the RNP1-NAs, the nucleic acid target-bound RNP1-NA becomes active and trans-cleaves at least one of the blocked nucleic acid molecules, thereby producing at least one unblocked nucleic acid molecule that can complex with one of the RNP2s; and
upon binding of the at least one unblocked nucleic acid molecule to one of the RNP2s, the unblocked nucleic acid molecule-bound RNP2 becomes active and trans-cleaves at least one more of the blocked nucleic acid molecules and at least one of the reporter moieties in a cascade;

allowing the cascade to continue; and
detecting the reporter moieties, thereby detecting the nucleic acid target of interest in the sample.