Patent ID: 11905552
Assignee: KECK GRADUATE INSTITUTE OF APPLIED LIFE SCIENCES
Field: Measurement (Instruments)
Classification: CPC C  G | IPC C  G

Claim 0:
1. A biosensor for assaying a target nucleic acid, the biosensor comprising:
a substrate comprising a substrate surface and at least two electrodes for passing current through the substrate, wherein the substrate surface comprises graphene;
a pyrenebutanoic acid (PBA) linker molecule comprising a pyrene ring moiety and a carboxylate moiety, wherein the linker molecule is conjugated to the substrate surface via non-covalent interaction between the pyrene ring moiety and the substrate surface;
a ribonucleoprotein (RNP) conjugated to the carboxylate moiety of the linker molecule via covalent bonding, wherein the RNP comprises Cas9 protein, wherein the linker molecule is joined to the Recombination UV C (RuvC) domain of the Cas9 protein, and wherein the RNP is immobilized to the substrate surface via the linker molecule; and
a guide ribonucleic acid (gRNA) forming a portion of the RNP and comprising a first sequence capable of binding to a protein portion of the RNP and a second sequence capable of binding to the target nucleic acid,
wherein conductivity of the substrate surface is modulated upon binding of the target nucleic acid to the gRNA, thereby enabling a detectable current change for sensing the target nucleic acid.