Patent ID: 11879162
Assignee: CALIFORNIA INSTITUTE OF TECHNOLOGY
Field: Biotechnology (Chemistry)
Classification: CPC C  Y | IPC C

Claim 21:
22. A medical system for detecting a presence or absence of at least one genetic variation in a first polynucleotide analyte in a sample comprising a first primer with a fluorophore attached thereto and a second primer with a quencher attached thereto, the medical system comprising:
a sensor;
a processor; and
a non-transitory computer-readable storage medium storing instructions that, when executed by the processor, cause the medical system to perform a method comprising:
a) measuring, using the sensor, a first signal generated by the fluorophore and quencher, wherein the first primer and the second primer are specific for the first polynucleotide analyte, and the fluorophore is different from the quencher;
b) measuring, using the sensor, a second signal generated by the fluorophore and the quencher from performing one or more first polymerization reactions with the first primer and the second primer using the first polynucleotide analyte as a template, wherein the one or more first polymerization reactions are performed after measuring the first signal;
c) comparing the first signal and the second signal to determine a first change in signal; and
d) determining the presence or absence of the at least one genetic variation in the first polynucleotide analyte by comparing the first change in signal to a second change in signal obtained by performing operations a) through c) for a second polynucleotide analyte without the at least one genetic variation and corresponding to the first polynucleotide analyte.