Patent ID: 11915795
Assignee: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA
Field: Computer technology (Electrical engineering)
Classification: CPC G  C | IPC C  G

Claim 0:
1. A method of profiling a sequence of a nucleic acid in a sample, the method comprising:
combining a sample comprising a nucleic acid with
one or more amplification primers,
one or more DNA intercalating dyes,
a reference dye, and
reagents for amplifying a nucleic acid to form a reaction mixture, wherein the reagents for amplifying a nucleic acid comprise a polymerase;

partitioning the reaction mixture into 10,000 or greater partitions, wherein each partition has a volume of 1 nL or less of the reaction mixture;
amplifying the nucleic acid in each partition to produce an amplification product, wherein the amplification product comprises zero, one, or more amplicons;
performing melt analysis of the amplification product in each partition, whereby a melt curve for the amplification product in each partition is produced; and
profiling the sequence of the nucleic acid,
wherein the step of profiling comprises: excluding one or more melt curves from a plurality of melt curves based on both: a comparison of a shape of one or more target amplification product melt curves to a plurality of melt curves from different nucleic acid sequences, and a comparison of a peak height of the one or more target amplification product melt curves to a peak height threshold, and
wherein the excluded one or more melt curves correspond to an off-target amplification product melt curve, wherein the off-target amplification product consists of one or more of a host nucleic acid molecule, an environmental nucleic acid molecule, or a degraded nucleic acid molecule.