Patent ID: 11921049
Assignee: nan
Field: Measurement (Instruments)
Classification: CPC C  G | IPC C  G

Claim 7:
8. The plurality of pairs of forward and reverse 3′ blocked primers for multiplex pyrophosphorolysis activated polymerization of claim 1, a) wherein the first forward or reverse 3′ blocked primer comprises the universal quencher attached to the first non-extendable, blocked nucleotide at the 3′ end, and wherein the first non-extendable, blocked nucleotide is an acyclonucleotide, and b) wherein the second forward or reverse blocked 3′ blocked primer comprises the universal quencher attached to the second non-extendable, blocked nucleotide at the 3′ end, and wherein the second non-extendable, blocked nucleotide is an acyclonucleotide.