Patent ID: 11898197
Assignee: WAINAMICS, INC.
Field: Chemical engineering (Chemistry)
Classification: CPC C  B | IPC B  C

Claim 5:
6. A device for performing a bioassay for one or more target polynucleotides comprising:
a sample chamber comprising a first inlet for accepting a biological sample, a vent port allowing the passage of air but not liquid, and an outlet connected to a first conduit;
a metering chamber (a) connected to and in fluid communication with a reagent chamber, (b) connected to and in fluid communication with the outlet of the sample chamber through the first conduit, (c) connected to a metering vent port, and (d) connected to and in fluid communication with a mixing chamber conduit;
a reagent chamber capable of containing assay reagents, the reagent chamber being connected to the metering chamber by a passage and connected to a reagent vent port allowing the passage of air but not liquid;
a mixing chamber for mixing the biological sample with the assay reagents, the mixing chamber having a top and a bottom and being in fluid communication with the metering chamber by a passage connecting the bottom of the mixing chamber to the metering chamber, the mixing chamber being connected at its top to a mixing vent port that allows the passage of air but not liquid, wherein the mixing chamber or the reagent chamber or both chambers contain a predetermined quantity of wax having a melting temperature such that the wax forms a bubble-preventing layer on a reaction mixture whenever the mixing chamber is above the melting temperature; and
a detection chamber is in fluid communication with the mixing chamber by a passage connecting the detection chamber to the bottom of the mixing chamber, and the detection chamber is connected at its top to a detection vent port that allows the passage of air but not liquid.