Patent ID: 11911770
Assignee: INDIAN INSTITUTE OF TECHNOLOGY, KHARAGPUR
Field: Measurement (Instruments)
Classification: CPC B  G  C | IPC B  C  G

Claim 0:
1. A set of primers suitable for desired SARS-CoV-2 detection with enhanced specificity and sensitivity wherein the set is selected from each of the primer sets directed to each gene selected from ORF 1b, N gene, E gene and RNaseP as hereunder:, Target
Primer 

Region
Set
Sequence (5′ to 3′)

ORF1b
F3
GCCATTAGTGCAAAGAATAGAGC

SEQ.

ID

No. 1

 

B3
GGCATGGCTCTATCACATTTAGG

SEQ.

ID

No. 2

 

FIP
5′[Btn]TAGCTCCTCTAGTGGCGGCTATTGCACC

(F1c +
GTAGCTGGTGTCTC

F2)

SEQ.

ID

No. 3

 

BIP
TGTAGTAATTGGAACAAGCAAATTCTATGGTGGCC

(B1c +
AACCCATAAGGTGAGGG

B2)

SEQ.

ID

No. 4

 

Loop
TTTTTGATGAAACTGTCTATTGGTCATAGTACTAC

F
AG

SEQ.

ID

No. 5

 

Loop
GGCACAACATGTTAAAAACTGTTTATAGTGATGTA

B
G

SEQ.

ID

No. 6

 

BLP
5′[6FAM]TTGGCACAACATGTTAAAAACTGTTTA

Probe
TAGTGATG

SEQ.

ID

No. 7

 

BLP
5′[6FAM]TTGGCACAACATGTTAAAAACTGTTTA

3′-
TAGTGATG 3′-[3dG]

mod

Probe

SEQ.

ID

No. 8

 

FLP
5′-

Probe
[6FAM]GCGGCTATTGATTTCAATAATTTTTGATG

SEQ.
AAAC

ID

No. 9

 

N
F3
ACAATGTAACACAAGCTTTCG

gene
SEQ.

ID

No. 10

 

B3
TTGGATCTTTGTCATCCAATT

SEQ.

ID

No. 11

 

FIP
5′[Btn]GGCCAATGTTTGTAATCAGTTCCTTAGA

(F1c +
CGTGGTC CAGAACAA

F2)

SEQ.

ID

No. 12

 

BIP
GCTTCAGCGTTCTTCGGAATCACCTGTGTAGGTCA

(B1c +
ACC

B2)

SEQ.

ID

No. 13

 

Loop
TGGTCCCCAAAATTTCCTTGG

F

SEQ.

ID

No. 14

 

Loop
CGCGCATTGGCATGGAAGT

B

SEQ.

ID

No. 15

 

BLP
5′-[6FAM] TTGGCATGGAAGTCACACCTTC

Probe

SEQ.

ID

No. 16

 

BLP
5′-[6FAM] TTGGCATGGAAGTCACACCTTC 

3′-
3′-[3dC]

mod

Probe

SEQ.

ID

No. 17

 

FLP
5′-[6FAM] GATTAGTTCCTGGTCCCCAAAATTT

Probe
CC

SEQ.

ID

No. 18

 

E
F3
TTGTAAGCACAAGCTGATG

gene
SEQ.

ID

No. 19

 

B3
AGAGTAAACGTAAAAAGAAGGTT

SEQ.

ID

No. 20

 

FIP
5′[Btn]CGAAAGCAAGAAAAAGAAGTACGCTAGT

(F1c +
ACGAACTTATGTACTCATTCG

F2)

SEQ.

ID

No. 21

 

BIP
GGTATTCTTGCTAGTTACACTAGCCAAGACTCACG

(B1c +
TTAACAATATTGC

B2)

SEQ.

ID

No. 22

 

Loop
ATTAACGTACCTGTCTCTTCCGAAA

F

SEQ.

ID

No. 23

 

Loop
ATCCTTACTGCGCTTCGATTGTGTG

B

SEQ.

ID

No. 24

 

BLP
5′-[6FAM] ATCCTTACTGCGCTTCGATTGTGTG

Probe

SEQ.

ID

No. 25

 

BLP
5′-[6FAM] ATCCTTACTGCGCTTCGATTGTGTG

3′-
3′-[3dG]

mod

Probe

SEQ.

ID

No. 26

 

FLP
5′-[6FAM] ATTAACTATTAACGTACCTGTCTCT

Probe
TCC

SEQ.

ID

No. 27

 

RNaseP
F3
TTGATGAGCTGGAGCCA

SEQ.

ID

No. 28

 

B3
CACCCTCAATGCAGAGTC

SEQ.

ID

No. 29

 

FIP
5′[Btn]GTGTGACCCTGAAGACTCGGTTTTAGCC

(F1c +
ACTGACTCGGATC

F2)

SEQ.

ID

No. 30

 

BIP
CCTCCGTGATATGGCTCTTCGTTTTTTTCTTACAT

(B1c +
GGCTCTGGTC

B2)

SEQ.

ID

No. 31

 

Loop
ATGTGGATGGCTGAGTTGTT

F

SEQ.

ID

No. 32

 

Loop
CATGCTGAGTACTGGACCTC

B

SEQ.

ID

No. 33

 

BLP
5′-[6FAM] CATGCTGAGTACTGGACCTCG

Probe

SEQ.

ID

No. 34

 

FLP
5′-[6FAM] ATGTGGATGGCTGAGTTGTT

Probe

SEQ.

ID

No. 35