Patent ID: 11971354
Assignee: MOLECULAR DEVICES, LLC
Field: Measurement (Instruments)
Classification: CPC G | IPC G

Claim 14:
15. The method of claim 1, wherein providing the one or more samples comprises contacting the one or more samples with:
a first antibody that specifically binds the first analyte,
a second antibody that specifically binds the second analyte,
a third antibody that specifically binds the third analyte,
a fourth antibody that specifically binds the fourth analyte,
a first fluorescent antibody conjugate that specifically binds the first antibody, wherein the first fluorescent antibody conjugate comprises the first fluorescent label,
a second fluorescent antibody conjugate that specifically binds the second antibody, wherein the second fluorescent antibody conjugate comprises the second fluorescent label,
a third fluorescent antibody conjugate that specifically binds the third antibody, wherein the third fluorescent antibody conjugate comprises the third fluorescent label including the UCP, and
a fourth fluorescent antibody conjugate that specifically binds the fourth antibody, wherein the fourth fluorescent antibody conjugate comprises the fourth fluorescent label including the infrared dye; and further comprising
incubating the one or more samples under conditions and for a time sufficient to allow the antibodies and the antibody conjugates to form immunocomplexes.