Patent ID: 11940381
Assignee: REVVITY HEALTH SCIENCES, INC.
Field: Measurement (Instruments)
Classification: CPC G  C | IPC C  G

Claim 15:
16. A method for reducing a false positive measurement in a biological sample using a multispectral analysis system, the method comprising:
calibrating the system using a first signal from a first calibration region of a calibration sample, wherein at least N fluorescent entities comprise a first fluorescent entity and N-1 different fluorescent entities, wherein the calibration sample comprises N calibration regions on a substrate, and wherein the N calibration regions comprises:
the first calibration region corresponding to the first fluorescent entity, wherein the first calibration region comprises a concentration of the first fluorescent entity in the first calibration region that is larger than a non-zero concentration of at least one other fluorescent entity in the first calibration region, wherein a fraction of the at least one other fluorescent entity in the first calibration region relative to a total amount of the first fluorescent entity and the at least one other fluorescent entity in the first calibration region is between 0.02 and 0.08, and wherein said concentration of the first fluorescent entity in the first calibration region and said fraction are configured to reduce spectral cross-talk of the fluorescence emission of the first fluorescent entity in the biological sample into a spectral emission channel for at least one of the N-1 different fluorescent entities in the biological sample; and
N-1 calibration regions, wherein each calibration region of the N-1 calibration regions corresponds to a single fluorescent entity of the N-1 different fluorescent entities and comprises a concentration of the single fluorescent entity that is larger than concentrations of any other fluorescent entities in the each calibration region;
wherein none of the N calibration regions comprises all of the N fluorescent entities; wherein at least one of the N calibration regions comprises multiple members of the N fluorescent entities; and wherein N is 2 or more;

measuring a fluorescence emission from the biological sample, wherein the fluorescence emission comprises a first measurement from the first fluorescent entity in the biological sample; and
reducing the false positive measurement arising from spectral cross-talk from the first fluorescent entity in the biological sample into the spectral emission channel for the at least one of the N-1 different fluorescent entities in the biological sample by correcting the first measurement from the biological sample with the first signal from the first calibration region.