Patent ID: 11971401
Assignee: BIOLOGICAL LIFE, INC
Field: Measurement (Instruments)
Classification: CPC G  C | IPC C  G

Claim 15:
16. A method for identifying medicinally active chemical entities in ethnobotanical substances, comprising the steps of:
(1) providing an ethnobotanical natural substance occurring naturally in nature, the ethnobotanical substance comprising an existing land or marine plant source identified as having a previously unidentified active agent associated with a medicinal effect on humans, in the form of previously unidentified medicinally active chemical entities which are the product of bodily function which occurs when the ethnobotanical substance is ingested by the human user, wherein the land or marine plant source consists of roots or fruits or seeds or bark or leaves;
(2) performing an in-vitro assay with the ethnobotanical substance with one or more of the following: (a) human intestinal preparations and (b) human liver preparations to produce an array of human chemical entities;
(3) performing an in-viva dosing of a plurality of animal species with said ethnobotanical substance;
(4) obtaining a biological fluid sample from the plurality of animal species following the in-vivo dosing;
(5) performing an analysis of the biological fluid samples to determine the presence of chemical entities in the biological fluid samples;
(6) wherein, if there is not a match between the chemical entities from the biological fluid samples and the array of human chemical entities from the in-vitro assay, then steps (7)-(9) are performed;
(7) performing an in-vitro assay of said ethnobotanical substance using one or more of the following: (a) animal intestinal preparations and (b) animal liver preparations from the plurality of animal species to produce an array of animal chemical entities; and
(8) wherein the matched chemical entities are potentially medicinally active chemical entities associated with efficacy of the ethnobotanical substance.