Patent ID: 11906721
Assignee: LEICA MICROSYSTEMS CMS GMBH
Field: Measurement (Instruments)
Classification: CPC G | IPC G

Claim 14:
15. A method for recording image stacks in a microscope, the method comprising:
manipulating at least one beam path by: determining a refractive index of a sample arranged in a sample volume and/or of an optical medium arranged in the sample volume, and setting at least one microscope parameter based on the determined refractive index for manipulating the beam path; and
capturing a change in a position of the sample with respect to an optical arrangement and/or capturing changes in an excitation wavelength before the at least one microscope parameter is set,
wherein determining the refractive index of the sample and/or of the optical medium arranged in the sample volume comprises:
(I) oblique irradiation of the sample volume with measurement light with respect to the optical axis using the optical arrangement;
(II) reflecting the irradiating light at a reflective element provided in the sample volume at a first position;
(III) imaging the reflected light onto a spatially resolving detector;
(IV) evaluating the signal detected by the detector with respect to a size and/or offset of the reflected light on the spatially resolving detector;
(V) displacing the reflective element along the optical axis to a second position and performing the method steps (III) and (IV); and
(VI) determining the refractive index based on the size and/or the offset of the reflected light for the first position and the second position of the reflective element.