Patent ID: 11859202
Assignee: UNIVERSITY OF MARYLAND, BALTIMORE
Field: Measurement (Instruments)
Classification: CPC C  B  G | IPC B  C  G

Claim 0:
1. A method of applying fluid flow to biological cells in a culturing well, comprising:
positioning a fluid flow device in a culturing well of a multiwell cell culture plate, the device comprising an elongate tubular body having an upper proximal end, a lower distal end, and a length therebetween, the distal end of the elongate tubular body configured for insertion into a well of a multiwell cell culture plate; at least one first source port positioned on the distal end; at least one first waste port positioned on the distal end; at least one second source port positioned on the elongate tubular body above the at least one first source port; at least one second waste port positioned on the elongate tubular body above the at least one first waste port; at least one source conduit positioned within the body connecting a single first source port out of the at least one first source port(s) to a single second source port out of the at least one second source port(s); and at least one waste conduit positioned within the body connecting a single first waste port out of the at least one first waste port(s) to a single second waste port out of the at least one second waste port(s), such that the at least one first source port and the at least one first waste port are positioned within the well; and
flowing a fluid through the device;
wherein the fluid applies a shear stress to the cells in the culturing well.