Patent ID: 11945879
Assignee: JEAN KADOUCHE
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 0:
1. A multispecific antibody construct comprising at least two Fab fragments with different CH1 and CL domains, wherein each Fab fragment recognizes a different epitope of interest, and wherein at least one Fab fragment is a mutated Fab fragment that comprises:
the VH and VL domains of an antibody recognizing an epitope of interest;
a CH1 domain which is derived from the CH1 domain of a human immunoglobulin by substitution of the leucine residue at position 143 of said CH1 domain with a glutamine residue and substitution of the serine residue at position 188 of said CH1 domain with a valine residue; and
a CL domain of the kappa type which is derived from the CL-kappa domain of a human immunoglobulin by substitution of the valine residue at position 133 of said CL-kappa domain with a threonine residue and substitution of the serine residue at position 176 of said CL-kappa domain with a valine residue; wherein the position numbers used for the CH1 and CL-kappa domains refer to Kabat numbering.