Patent ID: 11931426
Assignee: SNIPR TECHNOLOGIES LIMITED
Field: Pharmaceuticals (Chemistry)
Classification: CPC A  C | IPC A  C

Claim 0:
1. An in vitro method comprising
(A) combining
(a) a first DNA molecule, wherein the first DNA molecule is a double-stranded DNA molecule or a single-stranded DNA molecule,
(b) a further DNA molecule, wherein the further DNA molecule is single-stranded DNA (ssDNA); and
(c) an RNA-guided nuclease and a guide RNA;
wherein the nuclease is a Cas3 or Cpf1; and
the first DNA molecule comprises a first protospacer sequence (PS1) and a protospacer adjacent motif (PAM) (P1), wherein P1 is cognate to the nuclease for cutting PS1 at a first cut site (CS1) and wherein PS1 is adjacent to P1;

wherein the first DNA molecule is exposed to the guide RNA, wherein the guide RNA hybridizes to PS1;
(B) cutting the further DNA molecule with the nuclease to form a new marker; and
(C) detecting the marker.