Patent ID: 11898142
Assignee: THE BROAD INSTITUTE, INC.
Field: Measurement (Instruments)
Classification: CPC C  B  G  Y | IPC B  C  G

Claim 48:
49. The method according to claim 48, wherein said nucleic acid detection system comprises an RNA-based masking construct comprising a first and a second molecule, and wherein said lateral flow immunochromatographic assay comprises detecting said first and second molecule, optionally at discrete detection sites on a lateral flow strip; wherein said first molecule and said second molecule are optionally detected by binding to an antibody recognizing said first or second molecule and detecting said bound molecule, preferably with sandwich antibodies; and wherein said lateral flow strip optionally comprises an upstream first antibody directed against said first molecule, and a downstream second antibody directed against said second molecule, and wherein uncleaved RNA-based masking construct is bound by said first antibody if the target nucleic acid is not present in said sample, and wherein cleaved RNA-based masking construct is bound both by said first antibody and said second antibody if the target nucleic acid is present in said sample.