Patent ID: 11970544
Assignee: GENMAB A/S
Field: Pharmaceuticals (Chemistry)
Classification: CPC C  A | IPC A  C

Claim 27:
28. A nucleic acid construct, or combination of nucleic acid constructs, encoding a bispecific antibody comprising a first heavy chain and a first light chain comprising a first antigen-binding region which binds to human 5T4, and a second heavy chain and a second light chain comprising a second antigen-binding region which binds to human CD3, wherein:
(a) the first antigen-binding region comprises a variable heavy chain (VH) region comprising the amino acid sequence set forth in SEQ ID NO: 40 and a variable light chain (VL) region comprising the amino acid sequence set forth in SEQ ID NO: 44, and
(b) the second antigen-binding region comprises a VH region comprising the amino acid sequence set forth in SEQ ID NO: 68 and a VL region comprising the amino acid sequence set forth in SEQ ID NO: 60,
wherein the bispecific antibody comprises a human IgG1 Fc region, and wherein the first heavy chain comprises a constant region wherein the amino acid residues corresponding to L234, L235, D265 and K409 in a human IgG1 heavy chain according to EU numbering are F, E, A and R, respectively, and the second heavy chain comprises a constant region wherein the amino acid residues corresponding to L234, L235, D265 and F405 in a human IgG1 heavy chain according to EU numbering are F, E, A and L, respectively, and
wherein the first light chain and second light chain comprise light chain constant regions comprising the amino acid sequences set forth in SEQ ID NOs: 95 and 96, respectively.