Patent ID: 11913002
Assignee: WUHAN HEALTHGEN BIOTECHNOLOGY CORP.
Field: Biotechnology (Chemistry)
Classification: CPC C  A | IPC A  C

Claim 9:
10. A method of preparing a recombinant protein using the plant endosperm-specific expression promoter of claim 1, the method comprising:
1) constructing a rice endosperm cell-specific intermediate vector comprising incorporation into the vector the non-natural plant endosperm-specific expression promoter of claim 1;
2) synthesizing a nucleic acid encoding the recombinant protein;
3) operably ligating the vector of step 1) with the nucleic acid of step 2), to obtain a vector for recombinant protein expression mediated by the promoter of claim 1;
4) transforming a plant comprising an endosperm with the expression vector of step 3) and expressing the recombinant protein in the plant endosperm;
5) extracting and/or purifying the recombinant protein from the plant.