Patent ID: 11892446
Assignee: KING ABDULLAH UNIVERSITY OF SCIENCE AND TECHNOLOGY
Field: Measurement (Instruments)
Classification: CPC G | IPC G

Claim 0:
1. A method for assaying cell adhesion or cell rolling of multiple cell specimens comprising:
(a) contacting each cell specimen with a primary binding agent that specifically binds to an epitope on the surface of cells in the cell specimen;
(b) contacting each cell specimen with a second binding agent conjugated to a fluorescent label, wherein each cell specimen is contacted with a second binding agent comprising a different fluorescent label and the second binding agent specifically binds to the primary agent bound to an epitope on the surface of cells in the cell specimen;
(c) perfusing the cell specimens contacted with the second binding agents through a parallel plate flow chamber comprising a monolayer of cells;
(d) exciting the fluorescent labels with an amount of electromagnetic energy; and
(e) capturing real-time fluorescence images of the multiple cell specimens as the multiple cell specimens pass through the parallel plate flow chamber.