Patent ID: 11898165
Assignee: MINK THERAPEUTICS, INC.
Field: Measurement (Instruments)
Classification: CPC C  A  G | IPC C  G

Claim 0:
1. An isolated cell derived from a mouse lymphocyte, the isolated cell expressing a chimeric CD8 on the cell surface, wherein the isolated cell comprises:
(a) a first polynucleotide encoding a chimeric CD8α chain comprising an extracellular region of human CD8α and transmembrane and cytoplasmic regions of mouse CD8α,
(b) a second polynucleotide encoding a chimeric CD8β chain comprising an extracellular region of human CD8β and transmembrane and cytoplasmic regions of mouse CD8β, wherein the isolated cell expresses CD3 but does not express endogenous TCR on the cell surface; and
(c) one or more reporter system for measurement of TCR signaling;
wherein the chimeric CD8α chain comprises the amino acid sequence of SEQ ID NO: 1, and/or the chimeric CD8β chain comprises the amino acid sequence of SEQ ID NO: 2.