Patent ID: 11859247
Assignee: OXFORD NANOPORE TECHNOLOGIES PLC
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 0:
1. A method of purifying a transmembrane pore comprising at least one transmembrane construct, the transmembrane construct comprising a transmembrane protein pore subunit and a nucleic acid handling enzyme, wherein the subunit is covalently attached to the enzyme, wherein the subunit retains its ability to form a pore and wherein the enzyme retains its ability to handle nucleic acids, said method comprising:
(a) providing the at least one construct and other subunits required to form the pore;
(b) oligomerising the at least one construct and other subunits on lipid vesicles;
(c) contacting the vesicles with a non-ionic surfactant; and
(d) recovering the oligomerised pore.