Patent ID: 11884915
Assignee: AGILENT TECHNOLOGIES, INC.
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 0:
1. A prime-editing guide RNA (pegRNA) comprising:
a guide sequence that is capable of hybridizing to a target sequence in a target region of a nucleic acid;
a segment that interacts with a Cas protein;
a primer-binding site segment that can bind to the target region;
and a reverse transcriptase template segment including a sequence with desired edit(s) to the target region;
wherein the pegRNA comprises a 5′ end and a 3′ end, one of which is a prime-editing end and the other is referred to as a distal end, and
the pegRNA comprises at least two modified nucleotides within 5 nucleotides of the prime-editing end, said at least two modified nucleotides comprising 2′-O-methyl-3′-phosphonoacetate (“MP”).