Patent ID: 11898148
Assignee: CARRYGENES BIOENGINEERING, LLC
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 1:
2. The method of claim 1, further comprising the steps of:
identifying a second group of cells in which the first and the second genes of interest have been loaded onto the autonomously replicating nucleic acid sequence and the recyclable marker gene cassette is no longer present;
in the identified second group of cells, using the first recombination system, integrating a third delivery vector comprising the recyclable marker gene cassette and a third gene of interest into a third of the multiple recombination target sites on the autonomously replicating nucleic acid sequence; and with the second recombination system using signal sites gamma, excising the recyclable marker gene cassette from the autonomously replicating nucleic acid sequence wherein the third gene of interest remains on the autonomously replicating nucleic acid sequence.