Patent ID: 11898199
Assignee: UNIVERSAL DIAGNOSTICS, S.A.
Field: Biotechnology (Chemistry)
Classification: CPC C  G | IPC C

Claim 8:
9. A method of using methylation sensitive restriction enzyme quantitative polymerase chain reaction (MSRE-qPCR), the method comprising:
(a) contacting cell-free DNA from blood or plasma of a human subject suspected of having colorectal cancer and/or advanced adenoma with one or more methylation sensitive restriction enzymes;
(b) performing qPCR of enzyme-digested DNA, or amplicons thereof; and
(c) detecting the methylation status of at least one methylation site found within each of three or more differentially methylated regions (DMRs) of DNA, wherein the three or more DMRs comprise SEQ ID NO: 192, SEQ ID NO: 201, and SEQ ID NO: 210.