Patent ID: 11860129
Assignee: ZOMEDICA BIOTECHNOLOGIES LLC
Field: Measurement (Instruments)
Classification: CPC G | IPC G

Claim 12:
13. A method of increasing a dynamic range of a bulk acoustic wave (BAW) immunoassay, comprising:
contacting an analyte or an analyte molecule to which a tag is linked, a first recognition component, and an amplification element-linked recognition component to generate a complex comprising the first recognition component and the amplification element-linked recognition component,
wherein the first recognition component is immobilized relative to a surface of a first BAW resonator sensor and is configured to selectively bind: (a) the analyte, (b) the analyte molecule to which the tag is linked, (c) the tag, or (d) any combination thereof, to which the amplification element-linked recognition component is bound;

contacting an analyte or an analyte molecule to which a tag is linked, a second recognition component, and an amplification element-linked recognition component to generate a complex comprising the second recognition component and the amplification element-linked recognition component,
wherein the second recognition component is immobilized relative to a surface of a second BAW resonator sensor and is configured to selectively bind: (a) the analyte, (b) the analyte molecule to which the tag is linked, (c) the tag, or any combination thereof, to which the amplification element-linked recognition component is bound;

wherein the first recognition component has an affinity, capacity, or combination thereof for binding: (a) the analyte, (b) the analyte molecule to which the tag is linked, (c) the tag, or (d) any combination thereof, that is different from the affinity, capacity, or combination thereof for binding (a) the analyte, (b) the analyte molecule to which the tag is linked, (c) the tag, or (d) any combination thereof, of the second recognition component;
contacting an amplification element of the amplification element-linked recognition component with an amplification precursor under conditions to convert the amplification precursor into a molecule that adds mass at a surface of the first BAW resonator sensor, the second BAW resonator sensor, or both; and
measuring mass added at the surface of the first BAW resonator sensor, the second BAW resonator sensor, or both, thereby increasing the dynamic range of the BAW immunoassay.