Patent ID: 11938227
Assignee: ARCTURUS THERAPEUTICS, INC.
Field: Pharmaceuticals (Chemistry)
Classification: CPC A  C | IPC A

Claim 1:
2. A method of producing a lipid-encapsulated RNA nanoparticle, comprising the steps
a) flowing an aqueous solution comprising an RNA through a 1st tube having a first inner diameter (ID);
wherein the RNA comprises from about 6,000 to about 13,000 nucleotides;

b) flowing an ethanol solution comprising lipids through a 2nd tube having a second inner diameter (ID), at a flow rate of about 0.2 to about 1 times a flow rate of the aqueous solution through the 1st tube, wherein the lipids comprise a cationic lipid; and
c) mixing the ethanol solution with the aqueous solution;
wherein the first ID and second ID and flow rates through the 1st tube and 2nd tube are selected to produce a shear force sufficiently low to preserve the integrity of the RNA;
wherein the mixing produces an output solution flowing in the 1st tube comprising a turbulent flow of the RNA and the lipids in between about 10% to 75% ethanol v/v; and

wherein the lipid-encapsulated RNA nanoparticles have a bilayer structure.