Patent ID: 11913042
Assignee: WENMAN WU
Field: Pharmaceuticals (Chemistry)
Classification: CPC C  A | IPC A  C

Claim 4:
5. A method for preparing a highly active blood coagulation factor XI protein, comprising the steps of:
(1) inserting a human coagulation factor XI gene of human blood coagulation factor XI Gly397Ser mutant into a vector to obtain a recombinant vector;
(2) transforming a host cell with the above recombinant vector to obtain a cell clone expressing the recombinant blood coagulation factor XI Gly397Ser mutant;
(3) cultivating the above recombinant cell clone in a serum-free medium by continuous perfusion to induce expression of the recombinant blood coagulation factor XI Gly397Ser mutant protein;
(4) performing isolation, purification, filtration, final filling and lyophilization to obtain the expressed highly active blood coagulation factor XI Gly397Ser mutant protein, wherein the position of the mutation is relative to the wild type human factor XI sequence of SEQ ID NO: 8.