Patent ID: 11913039
Assignee: ALEXION PHARMACEUTICALS, INC.
Field: Pharmaceuticals (Chemistry)
Classification: CPC C  A | IPC C

Claim 2:
3. The method of claim 1, wherein:
(i) the culturing of the CHO cells in the culture medium is at a temperature of about 36° C. to about 38° C.;
(ii) the nutrient supplement is added to the culture medium at least one day after inoculation of CHO cells into the culture medium;
(iii) the nutrient supplement is added at more than 2 different times;
(iv) the culture medium is selected from the group consisting of serum-free medium; CD DG44 Medium; SFM4CHO Medium; and combinations thereof;
(v) the culturing of the CHO cells is in a 0.25 L to a 25,000 L bioreactor;
(vi) the temperature of the culture medium is decreased about 80 hours to about 120 hours after the inoculation;
(vii) step (d) of (I) or (II) occurs about 10 to about 14 days after inoculation;
(viii) the TSAC of the HCCF is about 2.2 mol/mol to about 3.6 mol/mol; and/or
(ix) the at least one additional purification step comprises at least one of harvest clarification, filtration, ultrafiltration, diafiltration, viral inactivation, affinity capture, and combinations thereof.