Patent ID: 11920169
Assignee: SANGAMO THERAPEUTICS, INC.
Field: Biotechnology (Chemistry)
Classification: CPC C  A | IPC C

Claim 23:
24. A dimer comprising first and second fusion molecules, the first fusion molecule comprising a DNA binding domain that binds to a first target site and a first wild-type or engineered cleavage domain and the second fusion molecule comprising a DNA-binding domain that binds to a second target site and a second wild-type or engineered cleavage domain, wherein the first and second cleavage domains dimerize when the DNA-binding domains are bound to the first and second target sites separated by 9 base pairs, wherein the first fusion molecule comprises an amino acid linker between the DNA-binding domain and the first cleavage domain, wherein the linker is selected from the group consisting of:, (SEQ ID NO: 276)

QPTWMKSPRKKRYPTGRLTAQ;

 

(SEQ ID NO: 277)

DPEYLSQRVPRRRQKPPMTRPY;

 

(SEQ ID NO: 278)

MEQRVRATNPRRRLAILTTESFM;

 

(SEQ ID NO: 279)

QSPNMIQRTPKRRTPLTIPLR;

 

(SEQ ID NO: 280)

AGNCARLTVRPPFSHP;

and

 

(SEQ ID NO: 281)

ERPPRSLASPPHALLL.