Patent ID: 11932676
Assignee: KLOTHO THERAPEUTICS, INC.
Field: Pharmaceuticals (Chemistry)
Classification: CPC C  A | IPC A  C

Claim 19:
20. A method of treating acute kidney injury or chronic kidney disease in a mammalian subject, the method comprising:
measuring a concentration of soluble Klotho protein in a bodily fluid of the subject;
calculating a first dosage of a pharmaceutical composition sufficient to raise the concentration of soluble Klotho protein in the bodily fluid of the subject above a predetermined level and to maintain the concentration of soluble Klotho protein in the bodily fluid of the subject above the predetermined level for a predetermined period of time, the pharmaceutical composition comprising:
a pharmaceutically acceptable carrier or excipient; and
an effective amount of a recombinant fusion protein comprising:
a synthetic signal sequence having at least 99% amino acid sequence identity to SEQ ID NO: 72;
a Klotho protein sequence having at least 99% amino acid sequence identity to SEQ ID NO: 4; and
an immunoglobulin Fc domain having at least 99% amino acid sequence identity to SEQ ID NO: 74; and
optionally, a linker disposed between the Klotho protein sequence and the immunoglobulin Fc domain;

administering the first dosage of the pharmaceutical composition to the mammalian subject, wherein the step of administering the first dosage is sufficient to raise the concentration of soluble Klotho protein in the bodily fluid of the subject above the predetermined level and to maintain the concentration of soluble Klotho protein in the bodily fluid of the subject above the predetermined level for the predetermined period of time, wherein the predetermined period of time is at least 6 hours; and
administering one or more subsequent dosage(s) of the pharmaceutical composition to the subject, wherein the step of administering one or more subsequent dosage(s) is sufficient to maintain the concentration of soluble Klotho protein in the bodily fluid of the subject above the predetermined level for an extended period of time, wherein the extended period of time is at least 24 hours.