Patent ID: 11898211
Assignee: LAWRENCE LIVERMORE NATIONAL SECURITY, LLC
Field: Biotechnology (Chemistry)
Classification: CPC C  G | IPC C  G

Claim 35:
36. A UO2F2-sensing genetic reportable component comprising:
one or more U sensitive promoters comprising a U-sensitive 1362 binding site having a DNA sequence, (SEQ ID NO: 1)

N1N2N3N4N5N6N7N8N9N10N11N12N13N14N15N16N17N18,

in which
N1 is C or T;
N2 is G or A;
N3 is T or C;
N4 is C;
N5 is A or G;
N6 is G or C;
N7 C or G;
N8 is any nucleotide;
N9 is any nucleotide;
N10 is any nucleotide;
N11 is any nucleotide;
N12 is T or C;
N13 is G;
N14 is T or C;
N15 is C;
N16 is A or C;
N17 is G; and
N18 is C or G,
and in which N1 to N17 are selected independently, together with
a U-sensing reportable molecular component,, wherein at least one of the one or more U-sensitive promoters and the U-sensing reportable molecular component, comprises an F-sensing riboswitch in a single output configuration wherein the U-sensing reportable genetic molecular component, is transcribed in presence of an effective amount of bioavailable fluoride,, and wherein the one or more U sensitive promoters and the U-sensing reportable molecular component are in a configuration wherein the one or more U sensitive promoters directly initiate expression of the U-sensing reportable molecular component in presence of bioavailable U and bioavailable Fluoride.