Patent ID: 11952627
Assignee: 10X GENOMICS, INC.
Field: Biotechnology (Chemistry)
Classification: CPC C  G | IPC C

Claim 0:
1. A method of identifying a location of an RNA in a biological sample, the method comprising:
(a) contacting the biological sample with an array comprising a plurality of capture probes, wherein a capture probe of the plurality of capture probes comprises a capture domain and a spatial barcode;
(b) releasing the RNA from the biological sample, wherein the RNA released from the biological sample is bound by the capture domain of the capture probe;
(c) extending a 3′ end of the capture probe using the RNA bound to the capture domain as a template, thereby generating an extended capture probe hybridized to the RNA;
(d) performing random-primed DNA synthesis, thereby replacing the RNA with a second strand DNA molecule hybridized to the extended capture probe to generate a double-stranded DNA product;
(e) ligating a double-stranded sequencing adaptor to the double-stranded DNA product and
(f) determining a nucleic acid sequence of (i) all or a part of the second strand DNA molecule or a complement thereof, and (ii) the spatial barcode or a complement thereof, and using the determined nucleic acid sequences of (i) and (ii) to identify the location of the RNA in the biological sample.