Patent ID: 11905557
Assignee: LIFE TECHNOLOGIES CORPORATION
Field: Biotechnology (Chemistry)
Classification: CPC C  B | IPC B  C

Claim 9:
10. A method comprising:
performing a single-stranded DNA sequencing assay to yield a product mixture;
introducing the product mixture to an inlet of a flow channel in a microfluidic chip, the flow channel comprising a high-surface area, convoluted material located along a length of the flow channel, the high-surface area, convoluted material having a first section and a second section at different locations along the length of the flow channel, wherein the first section is coated with an anion-exchange reagent configured to capture negatively-charged single stranded and with a size-exclusion reagent configured to prevent single-stranded DNA fragments larger than a preselected size from being captured, and wherein the second section is coated with a cation exchange reagent configured to capture positively-charged single-stranded DNA fragments;
flowing the product mixture into contact with the first section of the high-surface area, convoluted material;
capturing negatively-charged single-stranded DNA fragments from the product mixture at the first section to yield a first filtrate;
after the capturing of the negatively-charged single-stranded DNA fragments, flowing the first filtrate into contact with the second section of the high-surface area, convoluted material; and
capturing positively-charged single-stranded DNA fragments from the first filtrate at the second section to yield a second filtrate.