Patent ID: 11911457
Assignee: BHARAT BIOTECH INTERNATIONAL LTD.
Field: Pharmaceuticals (Chemistry)
Classification: CPC A  C  Y | IPC A  C

Claim 0:
1. A method for producing Enteroviral particles useful for preparation of vaccine compositions, wherein the method comprises the steps of:
a. producing Enteroviral particles in Vero cells;
b. purification of the Enteroviral particles by two step chromatography;
c. stabilization of purified Enteroviral particles; and
d. chemical inactivation of the Enteroviral particles in presence of formalin (0.025%) at 37° C. for 13 days in presence of an inactivation buffer to obtain Enteroviral particles;
wherein the said method comprises addition of one or more stabilizers before the inactivation step, wherein recovery of the Enteroviral particles is in the range of 80% to 99%, and wherein the two step chromatography is carried out by first running size exclusion chromatography followed by ion exchange chromatography.