Patent ID: 11965191
Assignee: CALIFORNIA INSTITUTE OF TECHNOLOGY
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 3:
4. The synthetic protein circuit of claim 1,
wherein the third protease comprises:
a first cut site specific for the first protease,
a second cut site specific for the second protease, and
an optional association domain of the third protease,
wherein the third protease is configured to be deactivated by cleavage of either of the first cut site specific for the first protease and the second cut site specific for the second protease;

wherein the first target protein comprises a first cut site specific for the third protease between the first degron of the first target protein and a distal part of the first target protein from the first degron, and wherein the first target protein is stabilized by cleavage of the first cut site specific for the third protease.