Patent ID: 11896034
Assignee: NOVOZYMES A/S
Field: Pharmaceuticals (Chemistry)
Classification: CPC A  C | IPC A  C

Claim 6:
7. A method of hydrolysing peptidoglycan in a bacterial cell wall, comprising treating the bacterial cell wall with one or more Glycoside Hydrolase Family 25 (GH25) polypeptides having lysozyme activity, wherein the polypeptide has improved activity compared to the activity of the polypeptide of SEQ ID NO: 39 and wherein the polypeptide is selected from the group consisting of:
(a) a polypeptide having at least 90% sequence identity to the polypeptide of SEQ ID NO: 3;
(b) a polypeptide having at least 85% sequence identity to the polypeptide of SEQ ID NO: 6;
(c) a polypeptide having at least 85% sequence identity to the polypeptide of SEQ ID NO: 9;
(d) a polypeptide having at least 85% sequence identity to the polypeptide of SEQ ID NO: 12;
(e) a polypeptide having at least 85% sequence identity to the polypeptide of SEQ ID NO: 18;
(f) a polypeptide having at least 85% sequence identity to the polypeptide of SEQ ID NO: 21;
(g) a polypeptide having at least 85% sequence identity to the polypeptide of SEQ ID NO: 24;
(h) a polypeptide having at least 85% sequence identity to the polypeptide of SEQ ID NO: 30;
(i) a polypeptide having at least 85% sequence identity to the polypeptide of SEQ ID NO: 33;
(j) a polypeptide having at least 85% sequence identity to the polypeptide of SEQ ID NO: 38;
(k) a variant of the polypeptide selected from the group consisting of SEQ ID NO: 3, SEQ ID NO: 6, SEQ ID NO: 9, SEQ ID NO: 12, SEQ ID NO: 18, SEQ ID NO: 21, SEQ ID NO: 24, SEQ ID NO: 30, SEQ ID NO: 33 and SEQ ID NO: 38, wherein the variant has lysozyme activity and comprises one or more amino acid substitutions, and/or one or more amino acid deletions, and/or one or more amino acid insertions or any combination thereof in 1-20 positions;
(l) a polypeptide comprising the polypeptide of (a), (b), (c), (d), (e), (f), (g), (h), (i), (j), or (k), and a N-terminal and/or C-terminal histidine tag (His-tag) and/or histidine-glutamine tag (HQ-tag);
(m) a polypeptide comprising the polypeptide of (a), (b), (c), (d), (e), (f), (g), (h), (i), (j), or (k) and a N-terminal and/or C-terminal extension of between 1 and 10 amino acids; and
(n) a fragment of the polypeptide of (a), (b), (c), (d), (e), (f), (g), (h), (i), (j), or (k) having lysozyme activity and having at least 90% of the length of the mature polypeptide.