Patent ID: 11952571
Assignee: MASSACHUSETTS INSTITUTE OF TECHNOLOGY
Field: Pharmaceuticals (Chemistry)
Classification: CPC C  A | IPC A  C

Claim 0:
1. A system capable of site-specifically integrating an exogenous nucleic acid into a mammalian cell genome at a desired target site, wherein the system comprises, in a single composition:
(a) a nucleic acid encoding a DNA binding nickase domain linked to a reverse transcriptase domain;
(b) a nucleic acid encoding at least two guide RNAs (gRNAs) comprising, from 3′ to 5′,
i. a primer binding sequence,
ii. a sequence complementary to one strand of an integration recognition sequence, and
iii. a target binding sequence,

 wherein the gRNA is capable of guiding the linked nickase-reverse transcriptase domains to the genomic target site;
(c) nucleic acid encoding an integration enzyme; and
(d) an exogenous nucleic acid linked to a sequence that is an integration cognate of the integration recognition sequence.