Patent ID: 11959069
Assignee: PROTEONIC BIOTECHNOLOGY IP B.V.
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 0:
1. A method for separating a polynucleotide insert from a polynucleotide vector backbone, the method comprising the steps of
i) providing a recombinant polynucleotide vector comprising the insert and the vector backbone, wherein the vector backbone comprises a first plurality of cleavage sites that divide the vector backbone into fragments, wherein all fragments are selected from a length of at most 1000 bp and at least 40 bp;
ii) contacting the recombinant vector with cleavage means capable of specifically cleaving the first plurality of cleavage sites to produce backbone fragments and the insert; and optionally,
iii) separating the insert from the backbone fragments of step ii).