Patent ID: 11874452
Assignee: ALENTIC MICROSCIENCE INC.
Field: Measurement (Instruments)
Classification: CPC G | IPC G

Claim 0:
1. A method comprising:
moving a second surface towards a first surface, on which a fluid specimen is arranged, to form a single layer of sample units in the fluid specimen in at least a portion of a space between the first surface and the second surface, wherein the sample units comprise a light absorbing substance;
illuminating the fluid specimen with light having a first wavelength;
capturing an image of the fluid specimen illuminated with the light having the first wavelength;
identifying, in the image, a first sample unit of the single layer of sample units;
determining, based on the image, a first intensity corresponding to light passing through the first sample unit;
determining, based on the image, a background intensity at a location of the first sample unit;
determining a path length through the single layer of sample units; and
based on the first intensity, the background intensity, the first wavelength, and the path length, determining a concentration of the light absorbing substance.