Patent ID: 11940441
Assignee: PRESIDENT AND FELLOWS OF HARVARD COLLEGE
Field: Measurement (Instruments)
Classification: CPC G  C  B | IPC C  G

Claim 0:
1. A method for culturing cells comprising:
1) providing a microfluidic device comprising:
a. a body comprising a central channel therein; and
b. an at least partially porous membrane positioned within the central channel, the membrane configured to separate the central channel to format at least one microchannel and at least one mesochannel, wherein a height ratio of the at least one mesochannel to the at least one microchannel ranges from 10:1 to about 50:1;

2) seeding human epithelial cells from a patient on said membrane facing the at least one mesochannel; and
3) culturing the seeded human cells from step 2) on said membrane submerged within a first liquid.