Patent ID: 11866728
Assignee: RENAGADE THERAPEUTICS MANAGEMENT INC.
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 23:
24. The gene editing system of claim 1, wherein the engineered retron ncRNA comprises:
A) a pre-msr sequence having a first complementary region of the retron ncRNA;
B) an msr sequence including an msr stem-loop structure;
C) an msd sequence including an msd stem-loop structure and comprising the HDR nucleotide sequence, wherein said msd sequence templates a single strand DNA product (RT-DNA) in the presence of the retron reverse transcriptase; and
D) a post-msd sequence having a second complementary region, wherein the first and second complementary regions form an a1/a2 duplex region of the retron ncRNA.