Patent ID: 11891620
Assignee: MAYO FOUNDATION FOR MEDICAL EDUCATION AND RESEARCH
Field: Pharmaceuticals (Chemistry)
Classification: CPC C  A | IPC C

Claim 0:
1. A cell culture medium composition for culturing primary cells, said cell culture medium composition, comprising:
a basal medium at a concentration of from about 20% (v/v) to about 99% (v/v);
a lyophilized human platelet lysate reconstituted at a concentration of from about 2.5% (v/v) to about 20% (v/v) and wherein said lyophilized human platelet lysate retains its growth properties after reconstitution;
an anticoagulant; and
a supplemental exogenous growth factor at a concentration of at least 1 fold to about 2,000 fold of the concentration of the growth factor present in the lyophilized human platelet lysate,
wherein the cell culture medium composition is free from bulking agents selected from a group consisting of sucrose, mannitol, trehalose and combinations thereof and free from an animal serum, and wherein the cell culture medium composition when culturing primary cells yields at least 80% confluence by 72 hours of the primary cells.