Patent ID: 11897959
Assignee: BIOATLA, INC.
Field: Pharmaceuticals (Chemistry)
Classification: CPC C  A | IPC A  C

Claim 0:
1. An antibody or antibody fragment that specifically binds to Axl protein, said antibody or antibody fragment comprising a heavy chain variable region including three complementarity determining regions having H1, H2, and H3 sequences, and a light chain variable region including three complementarity determining regions having L1, L2, and L3 sequences, wherein:
(a) the H1 sequence is X1GX2X3MX4 (SEQ ID NO: 1);
(b) the H2 sequence is LIKX5SNGGTX6YNQKFKG (SEQ ID NO: 2); and
(c) the H3 sequence is GX7X8X9X10X11X12X13X14DYX15X16 (SEQ ID NO: 3),, wherein
X1 is T or A or W,
X2 is H or A,
X3 is T,
X4 is N,
X5 is P,
X6 is S,
X7 is H,
X8 is Y,
X9 is E,
X10 is S,
X11 is Y,
X12 is E,
X13 is A,
X14 is M,
X15 is W, and
X16 is G, and
(d) the L1 sequence is KASQDX17X18SX19VX20 (SEQ ID NO: 4);
(e) the L2 sequence is X21X22X23TRX24T (SEQ ID NO: 5); and
(f) the L3 sequence is QEX25X26SX27X28X29X30 (SEQ ID NO: 6),, wherein
X17 is V,
X18 is S or V,
X19 is A,
X20 is A,
X21 is W,
X22 is Q,
X23 is D,
X24 is H,
X25 is H,
X26 is F,
X27 is T or P,
X28 is P,
X29 is L, and
X30 is T or R;
wherein the isolated antibody or antibody fragment has a higher binding affinity to human Axl protein in a tumor microenvironment of pH 5.8 to 7.0 in comparison with a non-tumor microenvironment of pH 7.2 to 7.8; and
the antibody or antibody fragment has a ratio of binding affinity to the human Axl protein in the tumor microenvironment to a binding affinity to the human Axl protein in the non-tumor microenvironment of at least 1.5:1.