Patent ID: 11926850
Assignee: CARGILL, INCORPORATED
Field: Biotechnology (Chemistry)
Classification: CPC C  Y | IPC C

Claim 0:
1. A genetically modified yeast comprising:
a heterologous gene encoding a polypeptide with monocarboxylic/monocarboxylate transporter activity, wherein the polypeptide with monocarboxylic/monocarboxylate transporter activity has an amino acid sequence with at least 90% sequence identity to at least one of SEQ ID NO: 24, SEQ ID NO: 26, SEQ ID NO: 28, SEQ ID NO: 76, or SEQ ID NO: 78;
one or more heterologous genes encoding a polypeptide with D-lactate dehydrogenase (DLD) activity, wherein the polypeptide with DLD activity has an amino acid sequence with at least 90% sequence identity to at least one of SEQ ID NO: 40, SEQ ID NO: 42, SEQ ID NO: 64, SEQ ID NO: 66, SEQ ID NO: 68, SEQ ID NO: 70, SEQ ID NO: 72, or SEQ ID NO: 74; and
one or more heterologous genes encoding a polypeptide with lactate dehydrogenase cytochrome b2 (CYB2) activity, wherein the polypeptide with CYB2 activity has an amino acid sequence with at least 90% sequence identity to at least one of SEQ ID NO: 15, SEQ ID NO: 17, SEQ ID NO: 54, SEQ ID NO: 56, SEQ ID NO: 58, SEQ ID NO: 60, or SEQ ID NO: 62;
wherein the yeast is capable of consuming lactate and producing ethanol at a titer greater than 80 g/L when the yeast is present in a fermentation medium comprising lactate and hexose.