Patent ID: 11906408
Assignee: THERMO FINNIGAN LLC
Field: Measurement (Instruments)
Classification: CPC G | IPC G

Claim 17:
18. A product for preparing a protein-containing sample for mass spectrometry analysis, comprising:
a closeable reaction vessel having an internal volume; and
a reagent mixture disposed within the internal volume of the closeable reaction vessel and comprising:
an immobilized proteolytic enzyme;
a reducing agent; and
an alkylating agent, wherein the reducing agent is tris(2-carboxyethyl) phosphine (TCEP) at a concentration between 1 mM and 5 mM, and wherein the alkylating agent is one of: chloroacetamide (CAA) at a concentration between 10 mM and 25 mM; iodacetic acid (IAC) at a concentration between 1 mM and 5 mM; and Iodoacetamide (IAA) at a concentration between 1 mM and 15 mM.