Patent ID: 11959088
Assignee: UNIVERSITÉ LAVAL
Field: Food chemistry (Chemistry)
Classification: CPC C  A | IPC A  C

Claim 15:
16. A method of using 6-benzylaminopurine (BAP) to increase yield of a heterologous protein of interest in a Nicotiana plant per gram fresh leaf weight and per plant, the heterologous protein of interest comprising an influenza hemagglutinin (HA) protein, the method comprising:
a) cultivating the Nicotiana plant in the presence of 100 to 700 ppm of the BAP under growth conditions of 12 hours to 24 hours light per day and a light intensity of 60 μmol/m2·s to 350 μmol/m2·s to produce a treated plant;
b) introducing one or more than one nucleic acid into the treated plant, the nucleic acid comprising a nucleotide sequence encoding the hemagglutinin (HA) protein, the nucleotide sequence operatively linked to a regulatory region that is active in the plant;
c) incubating the treated plant under conditions that permit the expression of the nucleotide sequence encoding the HA protein, wherein the yield of the HA protein per gram fresh leaf weight and per plant is increased when compared with the yield of the HA protein obtained from the same plant tissue of a similar plant that is grown under the same conditions, but that has not been cultivated in the presence of 100 to 700 ppm of the BAP; and followed by
d) harvesting one or more of S3 leaves S2 leaves and S1 leaves from the treated plant;, wherein the step of treating, step a), is performed from 40 days prior to the step of introducing, step b), up to 9 days prior to the step of introducing, step b).