Patent ID: 11969727
Assignee: CHINA MEDICAL UNIVERSITY
Field: Measurement (Instruments)
Classification: CPC B  G  C | IPC B  C  G

Claim 4:
5. A production method of a biochip comprising steps of:
producing a first cavity recessed in a top surface of a carrier by three-dimensional printing, reversal molding, laser processing, computer numerical control (CNC) processing or injection molding, the first cavity defining a first culture area, the first culture area having a bottom surface and a side wall;
producing a plurality of second cavities recessed in the bottom surface of the first cavity, each of the plurality of second cavities is a second culture area;
producing a through hole in a side wall of the carrier, the through hole extending through the side wall of the carrier and the side wall of the first culture area, the through hole positioned above the bottom surface of the first culture area;
placing a second biological cell in at least one second culture area of the plurality of second culture areas;
covering the at least one second culture area and the bottom surface of the first culture area with a middle layer having a third biological cell; and
placing a first biological cell in the first culture area.