Patent ID: 11883820
Assignee: APPLIED CELLS INC.
Field: Measurement (Instruments)
Classification: CPC B  A  C  G | IPC A  B  C  G

Claim 19:
20. A method for separating biological entities from a sample fluid using a microfluidic device comprising the steps of:
passing said sample fluid and a buffer fluid into a linear channel having an input end, an output end, a plurality of channel sections with different constant widths between said input and output ends, and one or more tapered transition sections with each tapered transition section interposed between two channel sections of said plurality of channel sections, wherein said buffer fluid flows along a center of said linear channel and said sample fluid flows along two side walls of said linear channel;
generating ultrasound in a form of a standing wave in said sample and buffer fluids in said plurality of channel sections;
passing a first group of said biological entities through a pair of side channels connected to one of said plurality of channel sections at a downstream end adjacent to one of said one or more tapered transition sections; and
passing a second group of said biological entities to a second outlet connected to said output end,
wherein said first group of said biological entities are smaller in physical size than said second group of said biological entities,
and
wherein said plurality of channel sections become progressively narrower from said input end to said output end.