Patent ID: 11926854
Assignee: MERCK PATENT GMBH
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 1:
2. The split intein-based affinity chromatography system of claim 1, wherein the N-intein polypeptide is part of a fusion protein comprising the N-intein polypeptide and a N-intein solubilization partner joined by a peptide bond wherein the N-intein solubilization partner polypeptide has a molecular weight of less than about 15 kDa, an Aliphatic Index value less than about 60, and a Grand Average Hydropathy value less than −1, and enhances solubility of the N-intein polypeptide compared to the N-intein polypeptide expressed in the absence of the solubilization partner polypeptide; and maintains the catalytic activity of the N-intein polypeptide when complexed with a C-intein domain, the C-intein domain not being a part of the fusion protein.