Patent ID: 11873474
Assignee: WILSON WOLF MANUFACTURING
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 0:
1. A method of removing media and cells from a cell culture device, the method comprising:
using an apparatus to reduce a volume of a liquid media in the cell culture device containing cells and liquid media, said apparatus comprising;
a gas delivery component,
a first fluid detection component that is capable of determining when a liquid media moving within a media removal conduit through a media removal port to the cell culture device is replaced by a gas, said first fluid detection component capable of sending a signal to a first fluid flow control component that is capable of terminating the fluid flow through the media removal conduit,
a second fluid detection component that is capable of determining when a liquid media moving within a cell removal conduit that is connected to the cell culture device is replaced by a gas, said second fluid detection component capable of sending a signal to a second fluid flow control component that is capable of terminating the fluid flow through the cell removal conduit; said cell culture device comprising a gas permeable bottom upon which cells reside, a media removal conduit and a media removal port, a cell removal conduit and a cell removal port, and a filter;
increasing the concentration of cells per milliliter of media within said cell culture device by connecting said gas delivery component to said filter,
connecting said first fluid detection component to said media removal conduit,
connecting said first fluid flow control component to said media removal conduit,
initiating gas delivery from said gas delivery component, whereby gas moves into said cell culture device and displaces the liquid media from said cell culture device into a media collection vessel connected to said media removal conduit, said first fluid detection component determines when the liquid media that is moving through said media removal conduit is replaced by gas and upon making that determination said first fluid detection component sends a signal to said first fluid flow control component which then terminates the flow of fluid through said media removal conduit;
removing cells from said gas permeable cell culture device by connecting said second fluid detection component to said cell removal conduit,
connecting said second fluid flow control component to said cell removal conduit, and initiating gas delivery from said gas delivery component, whereby gas moves into said cell culture device and displaces liquid media and cells from said cell culture device into a cell collection vessel connected to said cell removal conduit, said second fluid detection component determines when the fluid that is moving through said cell removal conduit is replaced by gas and sends a signal to said second fluid flow control component which then terminates the flow of fluid through said cell removal conduit.