Patent ID: 11946077
Assignee: EMENDOBIO INC.
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 17:
18. The method of claim 15, wherein the CRISPR nuclease effects a DNA break in a DNA strand adjacent to a NGR or NGG PAM sequence and effects a DNA break in a DNA strand adjacent a sequence that is complementary to the PAM sequence, or
wherein the CRISPR nuclease is a nickase formed by an amino acid substitution at D8, E502, H735 or D738 of SEQ ID NO: 5, and effects a DNA break in a DNA strand adjacent to the PAM sequence, or
wherein the CRISPR nuclease is a nickase formed by an amino acid substitution at D586, H587 or N610 of SEQ ID NO: 5, and effects a DNA break in a DNA strand adjacent to a sequence that is complementary to the PAM sequence.