Patent ID: 11970716
Assignee: ELIGO BIOSCIENCE
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 0:
1. A method of transferring, by transduction, conjugation or transformation, a genetic circuit from a donor bacterial cell to a target bacterial cell comprising:
contacting the donor bacterial cell with the target bacterial cell for a sufficient amount of time to allow transfer, by transduction, conjugation or transformation, of the genetic circuit into the target bacterial cell,
wherein said donor bacterial cell expresses a selected repressor protein that is not encoded by the genetic circuit and is absent in the target bacterial cell,
wherein the genetic circuit comprises a nucleic acid sequence of interest under the transcriptional control of a repressor binding sequence recognized by said repressor protein, and
wherein said repressor protein and/or the repressor binding sequence originate from a different bacterial species than the donor bacterial cell.