Patent ID: 11921107
Assignee: ORTHO-CLINICAL DIAGNOSTICS, INC.
Field: Measurement (Instruments)
Classification: CPC G | IPC G

Claim 8:
9. A method of performing an assay on a liquid sample for the detection of one more analytes of interest, the method comprising:
providing a liquid sample addition zone;
providing a reagent zone downstream from and in fluid communication with the liquid sample addition zone, the reagent zone containing a reagent material;
providing a detection zone in fluid communication with the reagent zone;
providing a wicking zone in fluid communication with the detection zone having a capacity to receive a liquid sample flowing from the detection zone, wherein the liquid sample addition zone, the reagent zone, the detection zone, and the wicking zone define a fluid flow path;
further providing a reagent addition zone along and in fluid communication with the fluid flow path downstream from the liquid sample addition zone and upstream of the detection zone, the reagent addition zone having a width that is greater than a width of the fluid flow path and less than a width of the liquid sample addition zone to receive a reagent from a reagent source;
adding the liquid sample containing the analyte of interest onto the liquid sample addition zone;
adding the reagent to the reagent addition zone, thereby interrupting flow of the liquid sample through the fluid flow path for controlling a sample size of the liquid sample within the fluid flow path;
moving the liquid sample by capillary action into the reagent zone, wherein the liquid sample dissolves the reagent material;
flowing the liquid sample away from the reagent zone with the dissolved reagent material therein into the detection zone by capillary action, wherein the analyte of interest is detected in the detection zone by reading a signal that is generated; and
flowing the liquid sample and any unbound material into the wicking zone.