Patent ID: 11935240
Assignee: ULTIVUE, INC.
Field: Measurement (Instruments)
Classification: CPC G  C | IPC C  G

Claim 0:
1. A quantitative method of validating at least one candidate imaging method or candidate imaging reagent for use in evaluating a biological sample for the presence of one or more targets comprising:
a. obtaining a first imaging signal using a first imaging method and/or first imaging reagents,
b. obtaining a second imaging signal using a second imaging method and/or second imaging reagents,
wherein
i. the first imaging method and the second imaging method are the same methods, and the first imaging reagents and the second imaging reagents are different reagents;
ii. the first imaging method and the second imaging method are different methods, and the first imaging reagents and the second imaging reagents are the same reagents; or
iii. the first imaging method and the second imaging method are different methods, and the first imaging reagents and the second imaging reagents are different reagents;

c. optionally aligning the first imaging signal and the second imaging signal to adjust for signal orientation, image parity, scale, rotation, and/or translation mismatch,
d. identifying a first imaging zone in both the first imaging signal and second imaging signal, wherein the first imaging zone in both imaging signals represents the same location in the biological sample;
e. comparing the first imaging zone in the first imaging signal and the first imaging zone in the second imaging signal by performing a first cross-correlation;, wherein the biological sample was treated with a fixative.