Patent ID: 11952624
Assignee: GENESENSE TECHNOLOGY INC., SHANGHAI (CN)
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 16:
17. A kit for sequencing a nucleic acid molecule, comprising:
a primer having a sequence complementary to a portion of the nucleic acid molecule and hybridizable to the portion of the nucleic acid as a template for chain extension;
four compounds which are respectively derivatives of nucleotides A, (T/U), C and G, wherein the hydroxyl (—OH) at the 3′-position of ribose or deoxyribose of each of the four compounds is protected by a reversible protecting group; each of the four compounds comprises, in sequence, from the nucleotide end: a first linker, a second linker, and a terminal molecular label binding to or reactive to a receptor in a detectable group comprising an luminescence-activating molecule and the receptor, the luminescence-activating molecule capable of causing emission of fluorescence in the presence of a suitable substrate without being excited by external photoexcitation; wherein the second linker in each of the four compounds are different, designated as L2A, L2T(U), L2C and L2G, and at least three of the four second linkers are each cleavable under a specific condition under which the second linker in other three of the four compounds as well as the first linker of any of the four compounds are not cleaved;
a suitable substrate, in presence of which the luminescence-activating molecule undergoes a fluorescence reaction and emits fluorescence;
one or more reagents to carry out cleaving reactions to cause one of L2A, L2T(U), L2C and L2G to cleave at a condition without cleaving the other of the L2A, L2T(U), L2C and L2G; and
one or more reagents to cleave the first linker and for removing the protecting group at the 3′-position of the ribose or deoxyribose of the four compounds.