Patent ID: 11866782
Assignee: FLUENT BIOSCIENCES INC.
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 6:
7. The method of claim 6 further comprising, prior to the step of amplifying and sequencing nucleic acid labels, lysing each of the single target cells contained within the monodisperse droplets to release a plurality of distinct mRNA molecules;
capturing the plurality of distinct mRNA molecules with the first capture probes;
extending the first capture probes with reverse transcriptase to form nascent first strand cDNAs;
annealing the nascent first strand cDNAs to the TSOs and copying the TSOs to generate cDNAs that are bound to the template particles and contain copies of the plurality of distinct mRNA molecules and the TSOs;
breaking the droplets to release the cDNA; and
amplifying and sequencing the cDNA to quantify the plurality of distinct mRNA molecules.