Patent ID: 11938475
Assignee: ILLUMINA CAMBRIDGE LIMITED
Field: Chemical engineering (Chemistry)
Classification: CPC B  C | IPC B  C

Claim 0:
1. A method, comprising:
applying a functionalized coating layer in depressions of a patterned flow cell substrate, wherein the depressions are separated by interstitial regions and wherein the patterned flow cell substrate includes a bonding region;
grafting a primer to the functionalized coating layer to form a grafted functionalized coating layer in the depressions;
applying a non-grafted hydrogel on at least the grafted functionalized coating layer, the non-grafted hydrogel being selected from the group consisting of crosslinked polyacrylamide, an agarose gel, and crosslinked polyethylene glycol; and
bonding a lid to the patterned flow cell substrate at the bonding region, thereby defining a flow channel between the patterned flow cell substrate and the lid.