Patent ID: 11946163
Assignee: KSQ THERAPEUTICS, INC.
Field: Biotechnology (Chemistry)
Classification: CPC C  G | IPC C  G

Claim 0:
1. A method of detecting the presence of one or more single nucleotide polymorphisms (SNPs) comprising:
(a) introducing an optimized gRNA library comprised of high-fidelity gRNAs into a population of cells that have been modified to express Cas9 protein;
(b) culturing the cells from step (a) under conditions permitting the expression of the gRNAs and the Cas9 protein;
(c) amplifying gRNA target sequences by polymerase chain reaction (PCR) from the cells of step (b) to obtain a plurality of target amplicons;
(d) sequencing the plurality of target amplicons;
(e) detecting the presence of genetic alterations in the target sequence; and
(f) determining the presence of one or more SNPs based on the presence of genetic alterations in the target sequence or in an adjoining sequence.