Patent ID: 11920186
Assignee: ULTIVUE, INC.
Field: Measurement (Instruments)
Classification: CPC C  G | IPC C  G

Claim 0:
1. A method comprising:
(1) contacting a sample being tested for the presence of one or more targets with one or more target-specific binding partners, wherein the sample is a cell or tissue, wherein each target-specific binding partner is linked to an antigen, and wherein target-specific binding partners of different specificity are linked to different antigens,
(2) optionally removing unbound target-specific binding partners,
(3) providing antigen-specific binding partners linked to docking strands,
(4) contacting the sample with antigen-specific binding partners linked to docking strands, wherein the docking strands are nucleic acid strands and different antigen-specific binding partners are linked to different docking strands, further wherein multiple antigen-specific binding partners bind to a single antigen,
(5) optionally removing unbound antigen-specific binding partners linked to docking strands;
(6) increasing the number of docking strands by a DNA amplification reaction and labeling of the docking strands using an optical label,
(7) imaging the sample to detect bound labeled antigen-specific binding partners,
(8) optionally extinguishing the signal from the optical label, and
(9)n optionally repeating steps (1)-(8), or any subset thereof.