Patent ID: 11965888
Assignee: DEPUY SYNTHES PRODUCTS, INC.
Field: Measurement (Instruments)
Classification: CPC G  C | IPC C  G

Claim 19:
20. A method of identifying an infection from pathogenic gram-negative bacteria within a gram-negative bacterial biofilm comprising cellulose comprising:
enzymatically digesting a sample of the gram negative biofilm to form a digested biofilm sample using an enzyme comprising cellulase for the digestion of extracellular matrix of the pathogenic gram negative bacteria;
separating the digested biofilm sample into a supernatant and a cell pellet;
combining the cell pellet with a cell lysing agent to release a quantity of endotoxins, wherein the quantity of endotoxins has a lower limit of zero;
concentrating the quantity of endotoxins, and,
performing a bacterial endotoxin test (BET) assay on the concentration to obtain a sample endotoxin value, wherein a sample endotoxin value that is greater than zero is indicative of the presence of the pathogenic gram-negative bacteria in the biofilm.