Patent ID: 11913028
Assignee: WISCONSIN ALUMNI RESEARCH FOUNDATION
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 14:
15. A zinc finger-based artificial transcription factor (ATF) library comprising a plurality of elements, wherein each element comprises:
one or more zinc finger domain-encoding sequences, wherein each of the one or more zinc finger domain-encoding sequences comprises a variable domain-encoding sequence encoding a sequence selected from the group consisting of SEQ ID NO:3 (X1-S-X2-X3-L-T-X6) and positions 3-9 of SEQ ID NO:69 (X1-S-X2-X3-R-K-X6), wherein each X1, X2, X3, and X6 in each instance is independently an encoded amino acid comprising one of R, H, K, D, Q, S, T, N, E, G, P, A, I, L, M, or V, wherein the one or more zinc finger domain-encoding sequences in each element comprises at least one zinc finger domain-encoding sequence comprising a variable domain-encoding sequence encoding SEQ ID NO:3 (X1-S-X2-X3-L-T-X6), wherein the variable domain-encoding sequence in at least one of the one or more zinc finger domain-encoding sequences in at least one element encodes one of SEQ ID NOS:123-341; and
an activation domain-encoding sequence.