Patent ID: 11880171
Assignee: COLDQUANTA, INC.
Field: Measurement (Instruments)
Classification: CPC G  H | IPC G  H

Claim 0:
1. A fluorescence detection process comprising:
forming an optical trap to localize molecular entities in a subregion of a cell, the subregion having a volume that is less than 1/10of the internal volume of the cell;
probing the trapped molecular entities using at least a first probe beam having a first probe wavelength so that the molecular entities emit fluorescence having a first fluorescence wavelength different from the first probe wavelength;
spectrally filtering the fluorescence using a spectral filter, the spectral filter being characterized by an effective angle of incidence on the spectral filter within which the filter can effectively block the first probe wavelength and transmit the first fluorescence wavelength, the filter being less effective at blocking the first probe wavelength or transmitting the first fluorescence wavelength at angles larger than the effective angle of incidence, at least half of the trapped molecular entities that emit fluorescence being within a conical volume defined by the effective angle of incidence; and
detecting spectrally filtered fluorescence transmitted by the spectral filter.