Patent ID: 11946066
Assignee: CALIFORNIA INSTITUTE OF TECHNOLOGY
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 19:
20. A method of controlling virus vector, comprising:
causing a polynucleotide of a viral vector to be internalized into a cell of a subject, wherein the polynucleotide encodes a nucleoprotein (N), a phosphoprotein (P), a matrix protein (M), an RNA-dependent RNA polymerase (L), and one or more transgenes; and
causing a stabilizing molecule to be present in or expressed by the cell, wherein at least one of the N, P, M, or L is a conditionally stable fusion protein comprising a stabilizing molecule binding domain, thereby the conditionally stable fusion protein changes from a destabilized state to a stabilized state when the stabilizing molecule binding domain binds the stabilizing molecule, and thereby a copy of the polynucleotide is generated from the polynucleotide; and/or

causing a protease inhibitor to be absent in the cell, wherein two of the N, P, M, or L are expressed as a protease fusion protein comprising a first protein and a second protein of the two of the N, P, M, or L separated from a protease by a first cut site and a second cut site, respectively, thereby the protease cuts the first cut site and the second cut site to generate the first protein and the second protein are in active states, and thereby a copy of the polynucleotide is generated from the polynucleotide.