Patent ID: 11860118
Assignee: BOARD OF REGENTS, THE UNIVERSITY OF TEXAS SYSTEM
Field: Measurement (Instruments)
Classification: CPC G  B | IPC B  G

Claim 16:
17. An aggregation-induced quantitative photothermal biosensing method of detecting a target, the method comprising:
irradiating a biosensor containing a sample with near-infrared radiation for a selected time, wherein the biosensor comprises a suspension of a hybridization buffer containing gold nanoparticles, sodium chloride, and single-stranded DNA probes configured to undergo DNA hybridization with a target, and wherein the near-infrared radiation is configured to induce a photothermal effect in any aggregated gold nanoparticles present in the biosensor;
determining a temperature change of the biosensor after irradiating the biosensor with the near-infrared radiation for the selected time; and
determining a concentration of the target present in the sample using the temperature change of the biosensor,
wherein the target is a genomic DNA sequence extracted from one of M. tuberculosis genomic DNA, B. pertussis, E. coli, breast cancer cells (MCF-7), cancer associated miRNA (miRNA-141), or parasitic infection related G. lamblia.