Patent ID: 11911765
Assignee: ILLUMINA, INC.
Field: Chemical engineering (Chemistry)
Classification: CPC B  C  G | IPC B  C  G

Claim 0:
1. A method, comprising:
flowing an incorporation reagent, through a reagent management system and a flow cell of an instrument, the flow cell having a first polynucleotide positioned therein, wherein the incorporation reagent adds a first base onto a sequence of bases, the sequence of bases comprising a second polynucleotide complementary to the first polynucleotide;
capturing an image, of an identification signal emanating from the first base, after the first base has been added onto the second polynucleotide;
flowing a cleavage reagent, through the reagent management system and flow cell, to remove a first terminator from the first base in order to enable a subsequent base in the sequence of bases to be added to the second polynucleotide; and
flowing a buffer reagent, through the reagent management system and flow cell in a plurality of cycles of consecutive forward and reverse flow directions to remove the cleavage reagent from the flow cell.