Patent ID: 11959909
Assignee: SOGANG UNIVERSITY RESEARCH FOUNDATION
Field: Measurement (Instruments)
Classification: CPC G  A  B  C | IPC A  B  C  G

Claim 0:
1. A basal ganglia-on-a-chip for screening therapeutic agents for dopamine-dependent brain and nervous system diseases, the basal ganglia-on-a-chip comprising:
graphene-conjugated magnetic nanoparticles patterned on a substrate;
a first layer comprising (i) a first hydrogel containing glutamatergic neurons and (ii) a second hydrogel containing GABAergic neurons, the first and second hydrogels being disposed in parallel on a pattern of the graphene-conjugated magnetic nanoparticles;
a second layer comprising a third hydrogel in contact with the second hydrogel, the third hydrogel containing GABAergic neurons and neuronal membrane protein-specific antibody-conjugated magnetic nanoparticles; and
a third layer comprising a fourth hydrogel in contact with the third hydrogel, the fourth hydrogel containing dopaminergic neurons and neuronal membrane protein-specific antibody-conjugated magnetic nanoparticles,
wherein the third hydrogel is on the second hydrogel and the fourth hydrogel is on the third hydrogel, and
wherein the GABAergic neuron of the third hydrogel and the dopaminergic neurons of the fourth hydrogel induced vertical growth towards the substrate,
wherein the first hydrogel and the second hydrogel have a concentration of gelatin methacrylate (GeIMA) of more than 3.0 w/w % and less than 5.0 w/w %, and
wherein the GeIMA is synthesized by adding 0.4 mL/g methacrylate to Dulbecco's phosphate-buffered saline (DPBS) in which 10 w/v % gelatin is dissolved.