Patent ID: 11932860
Assignee: KIKKOMAN CORPORATION
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 0:
1. A method for producing an alkaline phosphatase (ALP), comprising:
transforming an Aspergillus host with a vector comprising an open reading frame encoding an ALP and an Aspergillus strain-derived N-terminal secretory signal peptide,
culturing the Aspergillus host in appropriate culture medium and culture conditions to produce the ALP, and
wherein the amino acid sequence of the ALP comprises 90% or greater homology with either of the amino acid sequences set forth in SEQ ID NOs: 31 or 32 and has an amino acid substitution at position 122, 249, 251 and/or 410, and decreased protein glycosylation levels compared to a wild-type ALP, and wherein the Asn at position 122 is a substitution to Gln or Lys, the Asn at position 249 is a substitution to Gln, Asp or His, the Thr at position 251 is a substitution to Cys, and the Asn at position 410 is a substitution to Gln or Lys.