Patent ID: 11873515
Assignee: TF BIOSYN BIOTECHNOLOGY CO., LTD.
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 2:
3. A method for preparing the dehydrogenase mutant according to claim 1, comprising the following steps:
S1: ligating a starting gene encoding a Mesorhizobium 6-phosphoglycerate dehydrogenase (MesPDH) enzyme into a plasmid to obtain a recombinant plasmid, wherein the MesPDH enzyme comprises the amino acid sequence as shown in SEQ ID NO: 3;
S2: performing PCR amplification to produce a mutant gene as a product by contacting two mutation primers with the recombinant plasmid as a template, wherein PCR amplification results in the mutant gene encoding SEQ ID NO: 1 so that 283rd and 286th amino acids of SEQ ID NO: 3 encoded by the starting gene are both simultaneously mutated to encode valine, and recovering the mutant gene; and
S3: transforming the mutant gene into a host cell, culturing the host cell, and inducing expression of the mutant gene in the host cell to obtain the dehydrogenase mutant.