Patent ID: 11882815
Assignee: UNIVERSITY OF MASSACHUSETTS
Field: Biotechnology (Chemistry)
Classification: CPC A  C | IPC A  C

Claim 5:
6. A method for facilitating genome editing in cells of a pre-implantation embryo comprising an intact zona pellucida, the method comprising transducing the pre-implantation embryo in an oviduct of a subject with:
a first rAAV comprising a capsid harboring a nucleic acid comprising a promoter operably linked to a transgene encoding a guide RNA; and
a second rAAV comprising a capsid harboring a nucleic acid comprising a promoter operably linked to a transgene encoding a CRISPR/Cas-associated protein,
wherein the transducing does not comprise injection of the first rAAV and second rAAVs into the pre-implantation embryo, and wherein the transducing comprises direct injection of the first and second rAAVs into the oviduct of the subject.