Patent ID: 11933771
Assignee: SHIMADZU CORPORATION
Field: Measurement (Instruments)
Classification: CPC G | IPC G

Claim 4:
5. An analysis execution method performed by a liquid chromatograph that includes an analysis column, a junction and a fluorescence detector, including:
supplying a mobile phase to the fluorescence detector through the analysis column and the junction during an analysis of a sample;
suppling a fluorescent reaction liquid to the fluorescence detector through the junction during the analysis of the sample;
introducing the sample into the mobile phase at a position farther upstream than the analysis column during the analysis of the sample;
generating a chromatogram based on an output signal of the fluorescence detector during the analysis of the sample; and
determining a period from a point in time at which supply of the fluorescent reaction liquid starts to a point in time at which the supply of the fluorescent reaction liquid ends as a supply period during determination of an analysis condition,
wherein the supplying the fluorescent reaction liquid to the fluorescence detector through the junction includes starting supply of the fluorescent reaction liquid such that the fluorescent reaction liquid arrives at the junction later than a point in time at which supply of the mobile phase starts and before the sample introduced into the mobile phase arrives at the junction, and stopping the supply of the fluorescent reaction liquid after the sample passes through the junction,
the supplying the fluorescent reaction liquid to the fluorescence detector includes starting supply of the fluorescent reaction liquid at the start point in time of the determined supply period and stopping the su ply of the fluorescent reaction liquid at the end point in time of the supply period during the analysis of the sample, and
the determining the supply period includes:
detecting a period in which a peak of a component in the sample appears with respect to a point in time at which the sample is introduced into the mobile phase based on the generated chromatogram as a peak appearance period during the determination of the analysis condition;
calculating a moving time in which the sample moves from the junction to the fluorescence detector based on a total flow rate of the mobile phase, the sample and the fluorescent reaction liquid that are supplied from the junction to the fluorescence detector and a volume of a flow path from the junction to the fluorescence detector during the determination of the analysis condition; and
calculating a period including a period starting earlier than the detected peak appearance period by the calculated moving time as the supply period during the determination of the analysis condition.