Patent ID: 11891655
Assignee: BATTELLE MEMORIAL INSTITUTE
Field: Measurement (Instruments)
Classification: CPC C  G | IPC C  G

Claim 0:
1. A method, comprising:
exposing a subject or a sample to a probe for a time sufficient to allow the probe to bind to an enzyme involved in xenobiotic metabolism such that a probe-enzyme conjugate is formed; and
analyzing the probe-enzyme conjugate using a fluorescent detection technique, a colorimetric detection technique, a mass spectrometry technique, or a combination thereof;, wherein the probe comprises a compound of Formula II, , wherein
ERG, if present, is, , S(O)2OH, or an anionic form thereof;, P(O)(OH)2, or an anionic form thereof; a halogen; or —OPh-CH2—ONO2;
EBG has a structure satisfying a formula selected from Formulas IIAEBG-IIJEBG, wherein
Y is CH3 or CF3;
Y′ is O, NO2, or —N═NR″, wherein R″ is a dye or other reporting moiety;
t is an integer selected from 0 to 5; and
R′ is selected from aldehyde, ketone, ester, carboxylic acid, acyl, acyl halide, cyano, sulfonate, nitro, nitroso, quaternary amine, CF3, alkyl halide, or combinations thereof;

Linkera comprises an aliphatic group, a heteroaliphatic group, an aromatic group, an aliphatic-aromatic group, a heteroaliphatic-aromatic group, a heteroaromatic group, an aliphatic-heteroaromatic group, or a heteroaliphatic-heteroaromatic group;
each of Linkerb and Linkerc, if present, independently comprises an aliphatic group, a heteroaliphatic group, an aromatic group, an aliphatic-aromatic group, a heteroaliphatic-aromatic group, a heteroaromatic group, an aliphatic-heteroaromatic group, or a heteroaliphatic-heteroaromatic group;
R, if present, is a group comprising a carbamate or a carbonate;
each Tag, if present, independently comprises a functional group or molecule capable of generating a detectable signal;
each pTag, which is present if a Tag is not present, independently comprises a clickable functional group; and
each of n, m, p, and q independently is 0 or 1.