Patent ID: 11915796
Assignee: SEEGENE, INC.
Field: Computer technology (Electrical engineering)
Classification: CPC G  C | IPC C  G

Claim 22:
23. A non-transitory computer-readable storage medium containing instructions to configure a processor to perform a method for reducing inter-and intra-instrument signal variations of a data set of a target analyte in a sample, the method comprising:
(a) providing a normalization coefficient for calibrating the data set by using a reference value, a reference cycle and the data set;
wherein the data set is obtained from a signal-generating process for the target analyte using a signal-generating means; wherein the signal-generating process is a polymerase chain reaction (PCR) or a real-time polymerase chain reaction (real-time PCR); wherein the data set comprises a plurality of data points comprising cycles of the signal-generating process and signal values at the cycles;
wherein step (a) comprises the following steps:
performing PCR or real-time PCR on the sample;
obtaining the data set via by the PCR or real-time PCR;
selecting the reference cycle from the cycles of the data set; and
determining a signal value at the reference cycle;
wherein the reference value is a value arbitrarily determined from a real number except zero;
providing the normalization coefficient by defining a relationship between the reference value and a signal value at a cycle of the data set corresponding to the reference cycle;
wherein the relationship between the signal value at the cycle of the data set corresponding to the reference cycle and the reference value is a difference between the signal value at the cycle of the data set corresponding to the reference cycle and the reference value; and

(b) providing a calibrated data set by obtaining calibrated signal values by applying the normalization coefficient to the signal values of the data set.