Patent ID: 11884953
Assignee: SHUANG LIU
Field: Pharmaceuticals (Chemistry)
Classification: CPC C  A | IPC A  C

Claim 0:
1. A method for preparing an active protein peptide from an animal connective tissue, the method comprising:
washing and shredding the animal connective tissue, thereby obtaining shredded connective tissues;
extracting a crude protein product from the shredded connective tissues by adding a phosphate buffer solution, containing 0.05-3 M NaCl, of pH 6.0 to 8.0;
pulp grinding and/or homogenizing the crude protein product to obtain a homogenized sample;
adjusting a pH of the homogenized sample to 6.5-8.0 to obtain a connective tissue slurry;
conducting an enzymolysis of the connective tissue slurry, thereby obtaining a protein peptide enzymolysate;
carrying out filtration and nanofiltration of the protein peptide enzymolysate, thereby obtaining a filtrate;
degerming the filtrate, thereby obtaining a degermed sample; and
vacuum freeze-drying the degermed sample, thereby obtaining an active protein peptide.