Patent ID: 11891645
Assignee: NOVOZYMES A/S
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 9:
10. A protease variant, comprising a substitution at one or more positions corresponding to positions 20, 27, 29, 34, 40, 42, 45, 46, 50, 53, 54, 55, 58, 59, 60, 61, 62, 66, 69, 76, 81, 87, 93, 96, 98, 101, 102, 103, 109, 110, 115, 117, 122, 124, 125, 127, 128, 130, 136, 141, 142, 145, 146, 148, 149, 150, 153, 154, 157, 159, 161, 164, 167, 182, 183, 184, 185, 188, 199, 200, 207, 208, 209, 210, 211, 213, 216, 219, 228, 231, 237, 244, 249, 252, 253, 261, 266, 267, 272, 275, 276, 277, 280, 285, 288, 291, 292, 293, 294, 296, 299, 303, 304, 305, 306, 307, 308, 310, 317, 318, 319, 321, 326, 327, 336, 338, 341, 344, 345, 348, 356, 358, 359, 361, 362, 363, and 364 of the polypeptide of SEQ ID NO: 3, and the variant has protease activity and wherein the variant has at least 90%, but less than 100% sequence identity to the mature polypeptide of SEQ ID NO: 3, wherein the at least one substitution is selected from the group consisting of: G20L, G20R, T27S, S29A, S29C, S29L, S29N, S29P, S29R, A34L, A34N, A34R, D40S, D40Y, F42Y, K45L, K45Y, A46E, A46K, 550A, S50C, S50I, S50L, S50T, A53I, A53K, A53L, A53S, Q54L, F55R, D58M, I59M, I59P, 560C, S60E, S60P, S61D, S61P, S62C, S62N, S66F, S66L, S66M, S66T, T69C, D76A, D76G, D76K, D76L, D76N, D76P, D76V, D76Y, E81S, N87F, T93S, L96F, L96S, L96T, T98L, P101F, P101L, P101M, P101R, T102C, T102I, T103V, D109P, D110W, N115A, N115D, N115G, N115P, E117D, E117F, E117G, E117L, E117N, E117P, E117S, E117W, E117Y, 1122L, N124D, N124I, N124K, N124L, N124M, N124T, N124V, F125K, F125L, F125M, L127C, L127P, G128N, G128R, G128S, G128W, G128Y, S130D, S130F, S130I, S130N, L136C, G141A, G141C, G141F, G141L, G141M, G141Q, G141R, G141V, Q142A, Q142L, Q142M, Q142S, Q142W, N145F, N145G, N145I, N145L, N145P, N145R, N145S, N145V, N145W, T146A, T1460, T146H, T146I, T146M, T146N, T146Q, T146R, T146V, T146W, S148G, A149V, K150F, N153D, N153F, N153G, N153R, N153Y, Q154C, Q154F, Q154G, Q154I, Q154K, Q154L, Q154M, Q154R, Q154T, Q154V, Q154W, Q154Y, N157A, N157E, N157F, N157M, N157Y, Y159F, YI59G, Y159H, Y159S, Q161A, A164L, T167D, T167V, Q182F, S183A, S183F, S183L, S183M, A184D, A184F, A184H, A184I, A184K, A184L, A184Q, A184R, A184S, A184T, A184V, A184W, A184Y, H185A, H185L, H185P, H185S, N188L, N188Q, F199L, M200F, M200L, Q207F, G208H, G208Y, V209F, V209G, V209H, V209L, V209N, V209W, V209Y, S210E, S210L, S210R, S210T, S210Y, P211F, T213F, T213H, T213I, T213L, T213N, T213R, T213S, T213W, T213Y, A216F, A216L, S219M, I228F, I228H, I228L, I228M, I228Q, I228V, I228Y, Y231F, Y231S, S237F, S237I, S237K, S237L, G244L, G244N, G244P, G244R, G249C, N252F, N252K, N252S, R253C, V261A, V261G, V266C, V266R, D267S, S272I, S272K, S272L, S272M, S272Q, S272R, S272T, S272Y, Q275A, Q275F, Q275K, Q275L, Q275R, Q275V, T276F, T276R, T276V, I277L, I277V, D280F, D280H, D280M, D280N, D280S, D280T, A285L, A285S, T288C, T288I, T288V, S291V, V292C, I293M, I293Y, S294T, S294V, V296L, R299P, A303C, A303F, A303H, A303S, A303V, G304H, G304K, G304P, G304R, G304S, G304Y, K3050, K305F, K305H, K305I, K305L, K305M, K305S, K305T, K305Y, S306L, S306M, S306R, P307C, L308P, L308T, F310M, F310P, F310Y, S317A, S317C, S317G, S317H, S317I, S317K, S317L, S317R, S317W, S317Y, S318N, S318R, A319F, A319W, K321R, K321S, D326P, V327C, V327T, S336R, N338H, N338S, N338T, P341I, P341N, P341R, A344K, A344L, A344P, G345K, G345S, P348G, P348L, P348S, N356L, N356Y, A358F, A358L, A358P, K359C, K359L, K359S, K359W, L361E, L361M, L361P, L361R, L361S, L361T, L361V, T362A, T362C, T362H, T362I, T362K, T362L, T362M, T362P, T362Q, T362R, T362V, T362Y, A363E, A363F, A363L, A363M, A363V, V364F, V364I, V364L, V364M, and V364S, and wherein the substitution at the one or more positions provides a protease variant having an increase in specific activity measured as improvement factor, AIF, of at least 1.37.