Patent ID: 11913049
Assignee: RAMON GONZALEZ
Field: Biotechnology (Chemistry)
Classification: CPC C  Y | IPC C

Claim 10:
11. The method of claim 1, wherein the sequence of reactions for the formation of the product from the product precursor acetyl-CoA is selected:
a. a reverse beta oxidation (BOX-R) cycle comprised of:
i. an overexpressed thiolase that catalyzes the non-decarboxylative condensation of an acyl-CoA primer with a 2-carbon donor acetyl-CoA to produce a ß-ketoacyl-CoA;
ii. an overexpressed 3-oxoacyl-[acyl-carrier-protein] reductase or overexpressed 3-hydroxyacyl-CoA dehydrogenase that catalyzes the reduction of a ß-ketoacyl-CoA to a ß-hydroxyacyl-CoA;
iii. an overexpressed 3-hydroxyacyl-[acyl-carrier-protein] dehydratase or an overexpressed enoyl-CoA hydratase or 3-hydroxyacyl-CoA dehydratase that catalyzes the dehydration of a (3R)-ß-hydroxyacyl-CoA to a transenoyl-CoA;
iv. an overexpressed enoyl-[acyl-carrier-protein] reductase or acyl-CoA dehydrogenase or trans-enoyl-CoA reductase that catalyzes the reduction of said transenoyl-CoA to an acyl-CoA that is two carbons longer than said acyl-CoA primer; and
v. an overexpressed termination pathway that catalyzes the conversion of an intermediate from said BOX-R cycle to said product; or

b. a fatty acid biosynthesis (FAS) pathway comprised of:
i. an overexpressed acetyl-CoA carboxylase that catalyzes the conversion of acetyl-CoA to malonyl-CoA;
ii. an overexpressed malonyl-CoA-[acyl-carrier-protein] (“ACP”) transacylase that catalyzes the conversion of said malonyl-CoA to malonyl-ACP;
iii. an overexpressed β-ketoacyl-ACP synthase that catalyzes the decarboxylative condensation of said malonyl-ACP with an acyl-ACP primer to produce a ß-ketoacyl-ACP;
iv. an overexpressed 3-oxoacyl-ACP reductase that catalyzes the reduction of said ß-ketoacyl-ACP to a ß-hydroxyacyl-ACP;
v. an overexpressed 3-hydroxyacyl-ACP dehydratase that catalyzes the dehydration of a (3R)-ß-hydroxyacyl-ACP to a transenoyl-ACP;
vi. an overexpressed enoyl-ACP reductase that catalyzes the reduction of a transenoyl-ACP to an acyl-ACP that is two carbons longer than said acyl-ACP primer; and
vii. an overexpressed termination pathway that catalyzes the conversion of an intermediate from said FAS pathway to said product.