Patent ID: 11866790
Assignee: BEIJING ORIGINPOLY BIO-TEC CO., LTD.
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 0:
1. A detection primer set for early screening and diagnosis of ovarian cancer, wherein the detection primer set comprises target gene detection primers for detecting the methylated statuses of a combination of methylated fragments,
wherein the combination of methylated fragments comprises at least one methylated fragment of each of the following four genes, including PCDHB18P, CDO1, HOXA9 and LYPD5:
wherein the methylated fragment for the PCDHB18P gene consists of the nucleotide sequence of:
SEQ ID NO: 1,
SEQ ID NO: 2, or
SEQ ID NO: 3;
wherein the methylated fragment for the CDO1 gene consists of the nucleotide sequence of:
SEQ ID NO: 4,
SEQ ID NO: 5, or
SEQ ID NO: 6;
wherein the methylated fragment for the HOXA9 gene consists of the nucleotide sequence of:
SEQ ID NO: 7,
SEQ ID NO: 8, or
SEQ ID NO: 9;
and wherein the methylated fragment for the LYPD5 gene consists of the nucleotide sequence of:
SEQ ID NO: 10,
SEQ ID NO: 11, or
SEQ ID NO: 12,
wherein each of the target gene detection primers has added at its 5′ terminus a nucleotide sequence with a length of 5-10 bp which is complementary to and pairs with a nucleotide sequence at the 3′ terminus of the same primer to form a hairpin structure, wherein the last two bases at the 3′ terminus of each primer are a CG dinucleotide and the 5′ terminal sequence does not pair with the CG bases at the end of the 3′ terminus; and wherein the nucleotide sequence of each of the target gene detection primers has a Tm value which is 2-4° C. higher than the annealing temperature at which the target gene detection primer anneals to the methylated fragment of the target gene in a PCR reaction system.