Patent ID: 11913942
Assignee: IDEXX LABORATORIES, INC.
Field: Measurement (Instruments)
Classification: CPC G  C | IPC C  G

Claim 6:
7. A method for conducting an assay for an analyte in a sample, comprising:
(a) conducting a sample first reaction sequence comprising:
i. forming a sample first reaction mixture by contacting the sample with an anti-analyte antibody, a conjugate comprising the analyte and an enzyme, and a substrate that produces a signal when in contact with the enzyme, and
ii. measuring signal from the sample first reaction mixture;
iii. normalizing a reaction rate for the sample first reaction mixture by accounting for background associated with the sample first reaction mixture,

(b) conducting a sample second reaction sequence comprising,
i. forming a sample second reaction mixture by contacting the sample with the substrate and an anti-analyte antibody,
ii. measuring signal from the sample second reaction mixture;
iii. normalizing a reaction rate from the sample second reaction mixture by accounting for background associated with the sample second reaction mixture,

(c) subtracting the normalized rate of step (b)(iii) from the normalized rate of step (a)(iii) to provide a final reaction rate for the sample containing the analyte,
(d) using the final reaction rate to determine the amount of the analyte in the sample.