Patent ID: 11970786
Assignee: BIOO SCIENTIFIC CORPORATION
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 4:
5. A method for improving library sequencing quality in a sample, the method comprising:
forming a reaction composition in a partition, the reaction composition comprising:
at least one unique combination of a spike in control and corresponding at least one spike in control primer, the at least one spike in control primer comprising a synthetic, unique nucleotide barcode,
at least one nucleic acid adapter, and
the sample, the sample comprising a plurality of sample nucleic acids;

fragmenting the plurality of sample nucleic acids to generate sample nucleic acid fragments;
ligating the at least one nucleic acid adapter to the sample nucleic acid fragments to generate ligation products;
amplifying the reaction composition using the at least one spike in control primer to generate a multiplicity of amplification products comprising a multiplicity of ligation product amplicons and a multiplicity of spike in control amplicons, each spike in control amplicon comprising a nucleotide tag that is unique to the spike in control amplicons generated using the corresponding at least one spike in control primer;
quantifying the ligation product amplicons and the spike in control amplicons for sequencing;
detecting nucleic acid contamination in the sample, the contamination comprising an amplified spike in control not associated with the reaction composition, if the sequencing data exceeds a predetermined rejection parameter value indicative of one or more contaminating nucleic acids; and
excluding contaminated library fragment products from sequencing, wherein contaminated library fragment products include an amplified spike in control not associated with the reaction composition, and wherein excluding the contaminated library fragment products improves the library sequencing quality.