Patent ID: 11952619
Assignee: ILLUMINA, INC.
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 0:
1. A method of determining the density, the distribution, or the density and the distribution of an untagged primer nucleotide sequence grafted to a support comprising:
providing the support comprising:
a plurality of discrete wells;
a gel material positioned in each of the plurality of discrete wells;
a quality control tracer grafted to the gel material in each of the plurality of discrete wells; and
the untagged primer nucleotide sequence grafted to the gel material in each of the plurality of discrete wells;

wherein the quality control tracer comprises:
a cleavable nucleotide sequence comprising a remaining portion and a cleavable portion, the remaining portion being a non-reactive nucleotide sequence having a first end grafted to the gel material and a second end linked to the cleavable portion, and the cleavable portion including a cleavage site; and

a detectable label attached to a 3′ end of the cleavable nucleotide sequence;
the cleavable nucleotide sequence being selected from the group consisting of
5′-CATCTAGGCATCTAAGCATCAAUCTTACA-3′ (SEQ. ID NO. 3),
5′-polyT-CATCTAGGCATCTAAGCATCAAUCTTACA-3′ (SEQ. ID NO. 6),
5′-alkyne-CATCTAGGCATCTAAGCATCAAUCTTACA-3′ (SEQ. ID NO. 9),
5′-(alkyne)-(polyT or TTTTTT)AATGATACGGCGACCACCGAGAUCACAC-3′ (SEQ. ID NO. 19); and
wherein the quality control tracer and the untagged primer nucleotide sequence are grafted to the gel material in a predetermined ratio;
detecting a signal from the detectable label; determining the density and/or distribution of the quality control tracer based at least in part on the signal from the detectable label; and
determining the density and/or distribution of the grafted untagged primer nucleotide sequence based on the signal from the detectable label and the predetermined ratio.