Patent ID: 11898204
Assignee: KAPA BIOSYSTEMS, INC.
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 10:
11. A method of determining the sequence of a library for separately sequencing each strand of a target nucleic acid comprising the steps of:
a) in a reaction mixture, joining the ends of double stranded target nucleic acids to adaptors to form doubly-adapted target nucleic acids wherein the adaptor is a single strand forming a double stranded stem and a single-stranded loop, and the loop comprises a primer binding site and the adaptor further comprises strand synthesis termination site;
b) contacting the reaction mixture with an exonuclease thereby enriching for the doubly-adapted target nucleic acids and forming a library of doubly-adapted target nucleic acids;
c) contacting the library with a primer capable of hybridizing to the primer binding sites;
d) extending the primers thereby separately sequencing each strand of the target nucleic acids in the library, wherein extension is terminated at the strand synthesis termination site and does not proceed onto the complementary strand.