Patent ID: 11926815
Assignee: S2 GENOMICS, INC.
Field: Measurement (Instruments)
Classification: CPC C  B  G | IPC C  G

Claim 36:
37. A method of creating a microtissue comprising:
(a) providing a cartridge comprising:
(i) a preprocessing chamber comprising an opening and, positioned in the opening, a tissue disruptor configured for mechanical disruption of tissue;
(ii) a strain chamber comprising a strainer, wherein the strain chamber communicates with the preprocessing chamber;
(iii) a first processing chamber communicating with the strain chamber, wherein the first processing chamber comprises an element configured to produce a hanging drop of liquid from the strain chamber;
(iv) an optional second processing chamber communicating with the first processing chamber;
(v) one or more cartridge ports communicating with the processing chamber and the second processing chamber if present;

and further comprising a tissue;
(b) disrupting the tissue with the tissue disruptor to produce a cell suspension;
(c) straining the cell suspension with the strainer to produce strained cell suspension; and
(d) forming a hanging drop from the strained cell suspension using the element.