Patent ID: 11941861
Assignee: VENTANA MEDICAL SYSTEMS, INC.
Field: Measurement (Instruments)
Classification: CPC G | IPC G

Claim 15:
16. A system for removing contributions of autofluorescence in one or more channels of a multichannel image of a biological sample stained with one or more fluorescent labels, the system comprising:
one or more processors, and
one or more memories coupled to the one or more processors, the one or more memories to store computer-executable instructions that, when executed by the one or more processors, cause the system to perform operations comprising:
independently calculating a maximum autofluorescence correction in each channel of each pixel of the multichannel image, wherein the calculated maximum autofluorescence correction in each channel of each pixel is derived from: (i) at least two different retrieved autofluorescence spectral; and (ii) normalized measured spectra for pixels of the multichannel image; and
subtracting from each measured pixel spectrum of the multichannel image the calculated maximum autofluorescence correction in each channel for that pixel.