Patent ID: 11874280
Assignee: SONY GROUP CORPORATION
Field: Measurement (Instruments)
Classification: CPC G | IPC G

Claim 0:
1. A method for forming a covalent conjugate of a polymeric dye and a targeting moiety, the method comprising preparing a mixture comprising the polymeric dye, the targeting moiety, and a magnesium salt, and allowing the mixture to age for a time and at a temperature sufficient to form the covalent conjugate, wherein:
a) the polymeric dye comprises:
i) two or more fluorescent or colored moieties;
ii) at least one negatively charged phosphate group; and
iii) a reactive group Q capable of forming a covalent bond with a complementary reactive group Q′ on the targeting moiety,
wherein each of the two or more fluorescent or colored moieties are joined to an adjacent fluorescent or colored moiety via a linker comprising the at least one negatively charged phosphate group; and

b) the targeting moiety has affinity for a target analyte and comprises the complementary reactive group Q′.