Patent ID: 11889830
Assignee: ABS GLOBAL, INC.
Field: Basic materials chemistry (Chemistry)
Classification: CPC A | IPC A

Claim 16:
17. A method for dispensing a cryoprotectant (102) to a biological specimen (104), said method comprising:
a) fluidly coupling the cryoprotectant (102) to a controlled dispenser (115);
b) adding the biological specimen (104) into a vessel (110);
c) connecting the controlled dispenser (115) to the vessel (110) via a dispense tube (140), wherein a first end (142) of the dispense tube is fluidly connected to the controlled dispenser (115), and a second end (144) of the dispense tube is disposed inside the vessel (110) such that said second end (144) is contacting the biological specimen (104) or an internal surface (112) of the vessel;and
d) dispensing the cryoprotectant (102) into the vessel (110) at a desired flow rate via the controlled dispenser (115), thereby adding the cryoprotectant (102) to the biological specimen (104);
wherein by contacting the second end (144) of the dispense tube with the internal surface (112) of the vessel or the biological specimen (104), droplet formation is prevented and the cryoprotectant (102) is dispensed without being dripped into the vessel (110).