Patent ID: 11918600
Assignee: SUZHOU RIBO LIFE SCIENCE CO., LTD.
Field: Pharmaceuticals (Chemistry)
Classification: CPC A  C | IPC A  C

Claim 0:
1. A siRNA capable of inhibiting the expression of an HBV gene, comprising a sense strand and an antisense strand, wherein each nucleotide in the sense strand and the antisense strand is a modified nucleotide, wherein the sense strand and the antisense strand both comprise fluoro modified nucleotides and non-fluoro modified nucleotides; a “fluoro modified nucleotide” refers to a nucleotide formed by substituting the 2′-hydroxy of the ribose group of the nucleotide with a fluoro group, and a “non-fluoro modified nucleotide” refers to a nucleotide formed by substituting the 2′-hydroxy of the ribose group of the nucleotide with a non-fluoro group, or a nucleotide analogue; the sense strand comprises a nucleotide sequence 1; the antisense strand comprises a nucleotide sequence 2; the nucleotide sequence 1 and the nucleotide sequence 2 are at least partly reverse complementary to form a double-stranded region; wherein the nucleotide sequence 1 and the nucleotide sequence shown in SEQ ID NO: 1 have an equal length and no more than 3 nucleotide differences; and the nucleotide sequence 2 and the nucleotide sequence shown in SEQ ID NO: 2 have an equal length and no more than 3 nucleotide differences:

5′-GAAAGUAUGUCAACGAAUZ-3′  (SEQ ID NO: 1),

5′-Z′AUUCGUUGACAUACUUUC-3′  (SEQ ID NO: 2),

wherein Z is U, Z′ is A;
the nucleotide sequence 1 comprises a nucleotide ZA at the position corresponding to Z; the nucleotide sequence 2 comprises a nucleotide Z′B at the position corresponding to Z′; the nucleotide Z′B is the first nucleotide from 5′ terminal of the antisense strand; and
the fluoro modified nucleotides are located within the nucleotide sequence 1 and the nucleotide sequence 2; wherein in the direction from 5′ terminal to 3′ terminal, the nucleotides at positions 7, 8 and 9 in the sense strand of the nucleotide sequence 1 are fluoro modified nucleotides; the nucleotides at positions 2, 6, 14 and 16 in the antisense strand of the nucleotide sequence 2 are fluoro modified nucleotides; and the nucleotides at the other positions in the antisense strand and sense strand are non-fluoro modified nucleotides.