Patent ID: 11867690
Assignee: ASEDASCIENCES AG
Field: Measurement (Instruments)
Classification: CPC G | IPC G

Claim 0:
1. A data storage and retrieval system for a non-transitory computer readable storage medium comprised of fingerprints that are multiparametric response tensors characteristic of the effects of agents on cells determined by cell cytometry, wherein the fingerprints are provided by a cell cytometry method for measuring the effect of an agent on cells, said method comprising:
contacting aliquots of a population of cells with K different control conditions, where κ is at least 1, and with i different concentrations of an agent, where i is at least 1;
measuring p different phenotypic parameters, ψ, in individual cells of each aliquot, where p is at least 3 and, where ψp denotes a particular phenotypic parameter, thereby obtaining distributions Ck of the measured values for each control condition K for each phenotypic parameter ψp and distributions Si of the measured values for each concentration condition i for each phenotypic parameter ψp,
wherein the phenotypic parameters are measured in the individual cells by cell cytometry using a cell cytometer,
and wherein a multiparametric tensor fingerprint indicative of the response of the cells to the agent is produced from said measurements without gating by,
calculating pairwise distances d between the distributions of each control condition Ck and each concentration condition Si separately for each phenotypic parameter ψp, where

dκ,i(ψ)=D(Cκ(ψ),Si(ψ)), and D is a distance function;, combining the calculated distances to form a multiparametric response tensor A, A
  =
  
   [
   
    
     
      
       a
       
        [
        
         
          κ
          1
         
         ,
         
          ψ
          1
         
        
        ]
       
      
     
     
      ⋯
     
     
      
       a
       
        [
        
         
          κ
          1
         
         ,
         
          ψ
          p
         
        
        ]
       
      
     
    
    
     
      ⋮
     
     
      ⋱
     
     
      ⋮
     
    
    
     
      
       a
       
        [
        
         
          κ
          m
         
         ,
         
          ψ
          1
         
        
        ]
       
      
     
     
      ⋯
     
     
      
       a
       
        [
        
         
          κ
          m
         
         ,
         
          ψ
          p
         
        
        ]
       
      
     
    
   
   ]
  
 

wherein each of a[κ, 104] comprises all the pairwise distances calculated for one phenotypic parameter and one control:, a
   
    [
    
     κ
     ,
     ψ
    
    ]
   
  
  =
  
   [
   
    
     
      
       
        d
        
         κ
         ,
         1
        
        
         (
         ψ
         )
        
       
       =
       
        D
        ⁡
        (
        
         
          C
          κ
          
           (
           ψ
           )
          
         
         ,
         
          S
          1
          
           (
           ψ
           )
          
         
        
        )
       
      
     
    
    
     
      
       
        d
        
         κ
         ,
         1
        
        
         (
         ψ
         )
        
       
       =
       
        D
        ⁡
        (
        
         
          C
          κ
          
           (
           ψ
           )
          
         
         ,
         
          S
          2
          
           (
           ψ
           )
          
         
        
        )
       
      
     
    
    
     
      ⋮
     
    
    
     
      
       
        d
        
         κ
         ,
         1
        
        
         (
         ψ
         )
        
       
       =
       
        D
        ⁡
        (
        
         
          C
          κ
          
           (
           ψ
           )
          
         
         ,
         
          S
          n
          
           (
           ψ
           )
          
         
        
        )
       
      
     
    
   
   ], wherein the multiparametric response tensor A is a fingerprint characteristic of the effects of the agent on the cells that can be compared directly to other such fingerprints generated in the same way,
wherein the cytometry and the fingerprint are free of gating bias.