Patent ID: 11951474
Assignee: LIFE TECHNOLOGIES CORPORATION
Field: Measurement (Instruments)
Classification: CPC B  C  G  Y | IPC B  C  G

Claim 18:
19. An apparatus comprising:
a reagent source;
one or more wash solution sources;
a flowcell including a fluidics interface member mounted on and sealingly attached to a housing, the flowcell defining a plurality of separate flow chambers, each flow chamber having an inlet and an outlet, the inlet in fluid communication with the reagent source and the outlet connected to waste, each flow chamber having interior walls formed by attachment of the fluidics interface member to the housing;
the reagent source to provide a nucleotide solution to the flowcell; and
for each flow chamber of the plurality of separate flow chambers:
a passage in fluid communication with the inlet;
a junction in fluid communication with the passage, the reagent source, and a wash solution source of the one or more wash solution sources;
a valve to control passage of a wash solution to the junction; and

a fluidic circuit comprising:
a fluidics node having an outlet in fluid communication with the reagent source;
a plurality of fluid inlets;
at least one waste port; and
a plurality of passages, each fluid inlet of the plurality of fluid inlets in fluid communication with a unique passage of the plurality of passages, the at least one waste port in fluid communication with the fluidics node by each passage of the plurality of passages without intervening valves, the plurality of passages having a fluid resistance selected so that when a fluid is selected to flow solely through a single fluid inlet of the plurality of fluid inlets a first portion of the fluid flows to the at least one waste port via an associated passage uniquely associated with the single fluid inlet without entering the fluidics node and a second portion of the fluid flows via the associated passage to the fluidics node, a part of the second portion exits the fluidics node through the outlet and the remainder of the second portion exits the fluidics node to the at least one waste port through the plurality of passages other than the associated passage uniquely associated with the single fluid inlet, another fluid entering one of the plurality of passages from an unselected fluid inlet of the plurality of fluid inlets is directed to the at least one waste port without entering the fluidics node.