Patent ID: 11965208
Assignee: BECTON, DICKINSON AND COMPANY
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 15:
16. The method of claim 14, further comprising:
stochastically labeling the cell indexing oligonucleotides and nucleic acid target molecules in the third partition using a plurality of stochastic labels to generate a plurality of stochastically labeled cell indexing oligonucleotides and a plurality of stochastically labeled nucleic acid target molecules,
wherein the stochastic labels comprise a cellular label, wherein the stochastic labels associated with the same cell comprise the same cellular label, and wherein the stochastic labels associated with different cells comprise different cellular labels;
obtaining the sequencing data of the plurality of stochastically labeled cell indexing oligonucleotides and the plurality of stochastically labeled nucleic acid target molecules,
wherein obtaining the sequencing data of stochastically labeled cell indexing oligonucleotides comprises sequencing at least a portion of the cellular label and at least a portion of a cell indexing sequence; and
determining the spatial location of each nucleic acid target molecule by associating the cellular label of the third partition and the cell indexing sequence of the third partition, wherein the spatial location comprises the identity of the third partition from which the nucleic acid target molecule originated.