Patent ID: 11912988
Assignee: CHINA NATIONAL RICE RESEARCH INSTITUTE
Field: Organic fine chemistry (Chemistry)
Classification: CPC C | IPC C

Claim 0:
1. A method for constructing a simplified genomic library, wherein the method consists of:
performing a non-specific PCR amplification on a whole genome with one first pair of primers annealing at 25° C. to obtain random amplified fragments;
performing a specific PCR amplification on the random amplified fragments with one second pair of primers to obtain the simplified genomic library;
wherein each primer of the first pair of primers sequentially comprises a bridging sequence, a first tag sequence, and a sequence for non-specific amplification in the 5′ to 3′ direction, wherein the first pair of primers is
SEQ ID NO:4 and SEQ ID NO:8;
wherein each primer of the second pair of primers sequentially comprises a sequencing adaptor sequence, a sequencing primer sequence, and the bridging sequence in the 5′ to 3′ direction.