Patent ID: 11965172
Assignee: CALIFORNIA INSTITUTE OF TECHNOLOGY
Field: Biotechnology (Chemistry)
Classification: CPC C  A | IPC C

Claim 7:
8. A method of reversibly modifying a population, the method comprising:
obtaining a wild type organism,
positioning a two-vector system of claim 1 in the wild type organism,
generating an altered organism by inducing one or more sequence modifications in an essential gene by a DNA sequence modifying complex in the two-vector system that result in a defect in survival, growth control, fertility, or differentiation in one or more cells in the organism, and rescuing the defect in survival, growth control, fertility, or differentiation by a rescue transgene in the two-vector system,
introducing the altered organism in an environment wherein an increase in a frequency of the altered organism is desired relative to a frequency of the wild type organism in a population,
replacing the wild type organism with the altered organism in the population in the environment,
thereby obtaining a modified population,
reintroducing the wild type organism in an environment wherein an increase in a frequency of the wild type organism is desired relative to a frequency of the altered organism in the modified population,
replacing the altered organism with the wild type organism in the modified population in the environment,
thereby reversibly modifying the population.