Patent ID: 11969523
Assignee: CONTURA INTERNATIONAL A/S
Field: Medical technology (Instruments)
Classification: CPC A  C | IPC A  C

Claim 6:
7. A process for making the composition according to claim 1, comprising:
i. providing stem cells, a cell culture medium and a polyacrylamide hydrogel, wherein the polyacrylamide hydrogel comprises acrylamide monomers, a cross-linking agent, and N-hydroxyethyl acrylamide (HEA), wherein a weight percent of the HEA is greater than 0% and less than 0.02% of a total weight of the polyacrylamide gel,
wherein an elasticity modulus of the polyacrylamide hydrogel is 1 to 200 Pa,
wherein a molar ratio of the acrylamide monomers and the cross-linking agent is from 150:1 to 1000:1,
wherein the stem cells are mesenchymal stem cells; and
ii. mixing the components of i) thereby achieving the composition,
wherein the mesenchymal stem cells are encapsulated in the polyacrylamide hydrogel such that the polyacrylamide hydrogel supports viability and proliferation of the stem cells.