Patent ID: 11913031
Assignee: YISSUM RESEARCH DEVELOPMENT COMPANY OF THE HEBREW UNIVERSITY OF JERUSALEM LTD.
Field: Measurement (Instruments)
Classification: CPC C  G  B | IPC C

Claim 0:
1. An in-vitro method of sustaining a synchronized circadian rhythm in mammalian cells a in cell culture, the method comprising:
exposing the mammalian cells to a continuous flow of medium with a constant total volumetric flow rate in which at least two hormones are present in an oscillating manner with a periodicity of 24±4 hours,
wherein a first hormone and a second hormone of said at least two hormones are distinct,
wherein said exposing comprises flowing over said mammalian cells in culture a first medium comprising said first hormone, a second medium comprising said second hormone, a third medium devoid of said first hormone and said second hormone or a combination of said media and wherein changing concentrations of said at least two hormones are exposed to said mammalian cells by varying a volumetric flow rate of each of said media while keeping a constant total volumetric flow rate over the cells,
wherein an interval between a maximum concentration of said first hormone exposed to said mammalian cells and a maximum concentration of said second hormone exposed to said mammalian cells is at least 2 hours,
and wherein said first hormone is selected from the group consisting of: cortisol, testosterone, adiponectin, insulin, thyroxine (T4), and fibroblast growth factor 21 (FGF21), and said second hormone is selected from the group consisting of: melatonin, growth hormone, Triiodothyronine (T3), ghrelin, prolactin, TSH, vasopressin, and leptin,
thereby sustaining a synchronized circadian rhythm in mammalian cells in a cell culture.