Patent ID: 11965001
Assignee: CALIFORNIA INSTITUTE OF TECHNOLOGY
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 0:
1. A thermomer monomer comprising aft a modified amino acid sequence of TlpA from Salmonella typhimurium comprising a modified form of SEQ ID NO: 39 wherein position A341 is substituted with an aspartate and further comprising one of the following amino acid substitution mutations:
(a) E180 is substituted with R;
(b) R179 is substituted with E;
(c) E180 is substituted with R and E229 is substituted with R;
(d) R179 is substituted with E and R230 is substituted with E;
(e) E180 is substituted with R and R230 is substituted with E;
(f) R179 is substituted with E and E229 is substituted with R;
(g) E180 is substituted with R and E250 is substituted with R;
(h) 8179 is substituted with E and R251 is substituted with E;
(i) E180 is substituted with R and R251 is substituted with E;
(j) R179 is substituted with E and E250 is substituted with R;
(k) E229 is substituted with R;
(l) R230 is substituted with E;
(m) E250 is substituted with R; or
(n) R251 is substituted with E.