Patent ID: 11883475
Assignee: SANARIA INC.
Field: Pharmaceuticals (Chemistry)
Classification: CPC A  C  Y | IPC A  C

Claim 9:
10. A method of in vitro culturing the Plasmodium parasites of human host range of claim 1 during sporogonic development of the parasites, said method comprising:
a. culturing the Plasmodium parasites of human host range, which are at the gametocyte stage to zygote stage in the presence of red blood cells in an exflagellation culture medium;
b. collecting a mixture comprising zygotes, gametes, and gametocytes;
c. culturing the mixture on a substrate in an ookinete culture medium comprising Drosophila S2 cells, wherein the Plasmodium parasites of human host range differentiate to ookinetes;
d. exchanging the ookinete medium with an oocyst medium;
e. culturing the Plasmodium parasites of human host range in the oocyst medium, wherein the Plasmodium parasites of human host range differentiate to mature sporozoites; and
f. harvesting the Plasmodium parasites of human host range-produced thereby, wherein the Plasmodium parasites of human host range are at a mature sporozoite stage.