Patent ID: 11952587
Assignee: MEGAKARYON CORPORATION
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 0:
1. A method for producing platelets, comprising the steps of:
(1) culturing megakaryocytes in a platelet producing medium, and
(2) harvesting platelets obtained by the culturing;
wherein the step of culturing comprises:
(a) promoting a release of one or more platelet production promoting factor(s), wherein the factor(s) comprise MIF, NRDc, IGFBP2, TSP-1, PAI-1, or CCL5, from megakaryocytes by applying mechanical stress including turbulence by vortex; and/or
(b) externally adding platelet production promoting factors including MIF, NRDc, and IGFBP2,
wherein the step of culturing comprises step (b) and wherein step (b) further comprises externally adding at least one platelet production promoting factor(s) comprising TSP-1, PAI-1, or CCL5.