Patent ID: 11860170
Assignee: PROCISEDX INC.
Field: Measurement (Instruments)
Classification: CPC G | IPC G

Claim 0:
1. A homogeneous assay method for detecting the presence or amount of vascular cell adhesion protein 1 (VCAM-1) using only a sandwich assay and alpha-2-macroglobulin (A2M) using only a competitive assay in a sample, the method comprising:
in the sandwich assay, contacting the sample with a first anti-VCAM-1 antibody, which binds to a first epitope of VCAM-1, wherein the first anti-VCAM-1 antibody is labeled with a first donor fluorophore;
contacting the sample with a second anti-VCAM-1 antibody, which binds to a second epitope of VCAM-1, wherein the second anti-VCAM-1 antibody is labeled with a first acceptor fluorophore;
in the competitive assay, contacting the sample with an anti-A2M antibody, which binds to an epitope of A2M, wherein the anti-A2M antibody is labeled with a second donor fluorophore;
contacting the sample with an isolated A2M protein, wherein the isolated A2M protein is labeled with a second acceptor fluorophore;
incubating the sample for a time sufficient to obtain dual antibody labeled VCAM-1 and antibody labeled A2M; and
exciting the sample having dual antibody labeled VCAM-1 and antibody labeled A2M using one or more light sources to detect a single fluorescence emission signal associated with fluorescence resonance energy transfer (FRET) for the first acceptor fluorophore and a single fluorescence emission signal associated with FRET for the second acceptor fluorophore,
wherein the first and second acceptor fluorophores are different.