Patent ID: 11891414
Assignee: UNCHAINED LABS
Field: Chemical engineering (Chemistry)
Classification: CPC C  B  G | IPC B  C  G

Claim 5:
6. A method for automated exchange of buffer solutions from a biological sample comprising:
placing a sample plate comprising a plurality of reservoirs into a pressure chamber, wherein each reservoir of the plurality of reservoirs comprises a filter, and a filter attachment region comprising a thermal seal and an adhesive seal, and a ring shaped channel defined by a side wall and a ring shaped inner ridge of each reservoir, wherein the ring shaped channel comprises the thermal seal and is filled with an adhesive of the adhesive seal, and wherein the filter has a diameter configured to accommodate a height of the ring shaped inner ridge so that a peripheral edge of the filter may be secured to each reservoir at the filter attachment region; and
pressurizing a space in the pressure chamber above the reservoir plate to generate a pressure differential across the filter of at least about 30 psig to separate the biological sample from a first buffer solution.