Patent ID: 11891618
Assignee: REGENERON PHARMACEUTICALS, INC.
Field: Biotechnology (Chemistry)
Classification: CPC A  C | IPC A  C

Claim 27:
28. A method of making the mouse of claim 1, comprising:
(I) (a) modifying the genome of a pluripotent mouse cell to comprise the genetically modified endogenous Ttr locus;
(b) identifying or selecting the genetically modified pluripotent mouse cell comprising the genetically modified endogenous Ttr locus;
(c) introducing the genetically modified pluripotent mouse cell into a mouse host embryo; and
(d) gestating the mouse host embryo in a surrogate mother; or

(II) (a) modifying the genome of a mouse one-cell stage embryo to comprise the genetically modified endogenous Ttr locus;
(b) selecting the genetically modified mouse one-cell stage embryo comprising the genetically modified endogenous Ttr locus; and
(c) gestating the genetically modified mouse one-cell stage embryo in a surrogate mother.