Patent ID: 11866726
Assignee: EDITAS MEDICINE, INC.
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 0:
1. An isolated nucleic acid for homologous recombination with a target nucleic acid having a cleavage site, wherein:
(a) a first strand of the target nucleic acid comprises, from 5′ to 3′, P1-H1-X-H2-P2, wherein
P1 is a first priming site;
H1 is a first homology arm;
X is the cleavage site;
H2 is a second homology arm; and
P2 is a second priming site; and
(b) a first strand of the isolated nucleic acid comprises, from 5′ to 3′, A1-S1-P2′-N-P1′-S2-A2, wherein
A1 is a homology arm that is substantially identical to H1;
P2′ is a priming site that is substantially identical to P2;
N is a cargo;
P1′ is a priming site that is substantially identical to P1;
A2 is a homology arm that is substantially identical to H2;
wherein S1 is a first stuffer, wherein S2 is a second stuffer, and wherein each of S1 and S2 comprise a random or heterologous sequence having a GC content of approximately 40%.