Patent ID: 11926877
Assignee: THE BOARD OF TRUSTEES OF THE UNIVERSITY OF ILLINOIS
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 13:
14. A method of detecting polynucleotides from SARS-CoV-2 in a saliva sample comprising:
mixing the saliva sample with Tris Borate Ethylenediaminetetraacetic acid (TBE) in a 1:1 ratio prior to heating the saliva sample, to form a mixture;
heating the mixture at about 95 degrees Celsius for about 15-30 minutes;
after heating, contacting the mixture with polysorbate-20, wherein the polysorbate-20 is present in a final concentration of 0.5% or less by weight to provide a test sample; and
subjecting the test sample to conditions that amplify target polynucleotides of SARS-CoV-2 in the test sample using RT-qPCR, wherein the target polynucleotides comprise at least a portion of one or more of ORF1ab, N1-gene, N2-gene, and S-gene, and detecting the polynucleotides from SARS-CoV-2 in the saliva sample.