Patent ID: 11883429
Assignee: ENLIVEX THERAPEUTICS RDO LTD
Field: Pharmaceuticals (Chemistry)
Classification: CPC A  C | IPC A  C

Claim 20:
21. A method for producing a pharmaceutical composition comprising an irradiated mononuclear apoptotic cell preparation comprising a mononuclear cell population in an early apoptotic state, said method comprising the following steps,
(a) obtaining a mononuclear-enriched cell population of peripheral blood;
(b) freezing said mononuclear-enriched cell population in a freezing medium comprising an anticoagulant;
(c) thawing said mononuclear-enriched cell population;
(d) incubating said mononuclear-enriched cell population in an apoptosis inducing incubation medium comprising methylprednisolone at a final concentration of about 10-100 μg/mL and an anticoagulant, wherein induction produces an early apoptotic cell population;
(e) resuspending said induced early apoptotic cell populations obtained in step (d) in an administration medium; and
(f) inactivating said early apoptotic cell populations from step (e), wherein said inactivation comprises irradiating said apoptotic cell populations from step (e), and wherein said irradiation comprises gamma irradiation or UV irradiation at about 20-60 Grey units (Gy);, wherein said method produces a pharmaceutical composition comprising an irradiated mononuclear apoptotic cell preparation, wherein said irradiated mononuclear-enriched, early apoptotic cell preparation comprises
a decreased number of non-quiescent non-apoptotic cells;
a suppressed cellular activation of any living non-apoptotic cells; or
a reduced proliferation of any living non-apoptotic cells;
or any combination thereof compared with a preparation comprising non-irradiated cell populations.