Patent ID: 11971410
Assignee: ARIZONA BOARD OF REGENTS ON BEHALF OF ARIZONA STATE UNIVERSITY
Field: Measurement (Instruments)
Classification: CPC G  C  A | IPC C  G

Claim 17:
18. A method of modifying a treatment with an immunotherapeutic of a subject, comprising:
contacting a biological sample comprising antibodies from a subject to a frameshift array comprising a plurality of tumor-associated frameshift peptides,
wherein the tumor-associated frameshift peptides comprise a frameshift peptide longer than 15 amino acids represented by 2 or more tumor-associated frameshift peptides on the frameshift array, and
wherein the tumor-associated frameshift peptides comprise frameshift peptides derived from indels arising during RNA transcription or from mis-splicing of exons;
measuring binding of antibodies having affinity to one or more of the tumor-associated frameshift peptides in the contacted biological sample by detecting a fluorescent label;
for each of the plurality of tumor-associated frameshift peptides, quantifying levels of binding of the antibodies having affinity to one or more of the tumor-associated frameshift peptides by determining a quantitative amount of fluorescence; and
modifying a treatment of the subject with the immunotherapeutic.