Patent ID: 11866712
Assignee: CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE
Field: Measurement (Instruments)
Classification: CPC C  G | IPC C  G

Claim 0:
1. A reporter system for the trans-translational activity of a bacterial species selected from the group consisting of Escherichia coli, Enterococcus faecium, Staphylococcus aureus, Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa, Enterobacter cloacae, and Mycobacterium tuberculosis, comprising:
a nucleotide sequence encoding the first 10 domains of GFP and not comprising a stop codon;
a nucleotide sequence encoding the SmpB protein of said bacterial species; and
a modified tmRNA, wherein the modified tmRNA is the tmRNA of said bacterial species, in which the portion of sequence encoding the proteolysis tag is replaced with a nucleotide sequence consisting of sequence SEQ ID NO: 19 which encodes the 11th domain of GFP of sequence SEQ NO: 18.