Patent ID: 11938227
Assignee: ARCTURUS THERAPEUTICS, INC.
Field: Pharmaceuticals (Chemistry)
Classification: CPC A  C | IPC A

Claim 0:
1. A method of producing a lipid-encapsulated RNA nanoparticle, comprising the steps
a) flowing an aqueous solution comprising an RNA through a 1st tube having an inner diameter (ID) of from about 0.01 inches to about 0.08 inches; wherein a pH of the aqueous solution is in a range from about 3.0 to about 4.5 with an optional NaCl concentration of up to about 300 mM;
wherein the RNA comprises from about 6,000 to about 13,000 nucleotides;

b) flowing an ethanol solution comprising lipids through a 2nd tube having an ID of from about 0.01 inches to about 0.04 inches at a flow rate of about 0.2 to about 1 times a flow rate of the aqueous solution through the 1st tube, wherein the lipids comprise a cationic lipid; and
c) mixing the ethanol solution with the aqueous solution;
wherein the mixing produces an output solution flowing in the 1st tube comprising a turbulent flow of the RNA and the lipids in about 10% to 75% ethanol v/v; and
wherein the lipid-encapsulated RNA nanoparticles have a bilayer structure.