Patent ID: 11925725
Assignee: THE JOHNS HOPKINS UNIVERSITY
Field: Medical technology (Instruments)
Classification: CPC A  B  C | IPC A  B  C

Claim 9:
10. A method of making a porous extracellular matrix (ECM) scaffold, the method comprising:
(a) providing a 3D printable extracellular matrix (ECM) mixture comprising 30% by weight of ECM material having a particle size less than 40 pm and from 70% by weight of a biocompatible synthetic polymer material, wherein the biocompatible synthetic polymer material comprises poly(ε-caprolactone) (PCL), and wherein the ECM material comprises a mineral phase and a collagen phase of decellularized bone;
(b) printing the porous ECM scaffold with a 3D printing process; and
(c) seeding the 3D-printed porous ECM scaffold with a plurality of human adipose-derived stem cells (hASCs).