Patent ID: 11911452
Assignee: SANOFI PASTEUR INC.
Field: Pharmaceuticals (Chemistry)
Classification: CPC A  C | IPC A  C

Claim 0:
1. A method for preparing an immunogenic serotype 9N conjugate, the method comprising:
(a) lysing a bacterial cell producing Streptococcus pneumoniae serotype 9N capsular polysaccharide by fermentation;
(b) purifying Streptococcus pneumoniae serotype 9N capsular polysaccharide from the lysed bacterial cell;
(c) activating the Streptococcus pneumoniae serotype 9N capsular polysaccharide by reacting with an oxidizing agent to achieve a degree of oxidation of 2-19, wherein the activated Streptococcus pneumoniae serotype 9N capsular polysaccharide has a molecular weight of 200-700 kDa; and
(d) mixing the activated Streptococcus pneumoniae serotype 9N capsular polysaccharide with a CRM197 carrier protein to conjugate the CRM197 carrier protein to the activated Streptococcus pneumoniae serotype 9N capsular polysaccharide and thereby form the immunogenic serotype 9N conjugate.