Patent ID: 11873507
Assignee: REPLICATE BIOSCIENCE, INC.
Field: Pharmaceuticals (Chemistry)
Classification: CPC C  A | IPC A  C

Claim 0:
1. A nucleic acid construct comprising a nucleic acid sequence encoding a modified alphavirus genome or self-replicating RNA (srRNA) obtained from an alphavirus genome or srRNA, wherein said modified alphavirus genome or srRNA is produced by replacing at least a portion of the nucleic acid sequence encoding the viral structural proteins of the alphavirus genome or srRNA with a coding sequence for a polypeptide construct comprising: a) a coding sequence for a p35 subunit of interleukin 12 (p35 or IL-12A) or a functional variant thereof; b) a coding sequence for a p40 subunit of interleukin 12 (p40 or IL-12B) or functional variant thereof; and c) a coding sequence for an interleukin-1 receptor antagonist (IL-1RA) or a functional variant thereof, wherein the coding sequences for IL-12A, IL-12B, and IL-1RA, or functional variants thereof, are operably linked to one another, and wherein the coding sequences for IL-12A, IL-12B, and IL-1RA express protein that is functional in a bioactivity assay, and wherein the nucleic acid sequence has at least 80%, at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% sequence identity to the nucleic acid sequence of SEQ ID NO: 10.