Patent ID: 11859245
Assignee: INSTITUTE OF ZOOLOGY, CHINESE ACADEMY OF SCIENCES
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 11:
12. The method of claim 11, wherein the PCR tube is treated under an extension reaction program and a PCR reaction program consecutively; the extension reaction program comprises incubation at 25° C. for 10-25 min, and denaturation at 94° C. for 5 min; the PCR reaction program comprises: a) denaturation at 94° C. for 2 min; b) denaturation at 94° C. for 30 s; c) annealing at 57-60° C. for 30 s; d) extension at 72° C. for 30 s; e) repeating operations b)-d) 45-50 times; and f) extension at 72° C. for 10 min.