Patent ID: 11921118
Assignee: THE BOARD OF TRUSTEES OF THE LELAND STANFORD JUNIOR UNIVERSITY
Field: Measurement (Instruments)
Classification: CPC C  G | IPC C  G

Claim 2:
3. A method for labeling a protein or a portion of proteins produced by a cell, the method comprising:
(a) introducing into the cell two or more variant aminoacyl-tRNA synthetases that recognize one or more noncanonical amino acids, wherein the two or more variant aminoacyl-tRNA synthetases comprise two or more of ScTyrY43G of SEQ ID NO:4, the MmPheT413G of SEQ ID NO:5, and HsPheT413G of SEQ ID NO:6, and wherein the one or more noncanonical amino acids comprise 3-azido-L tyrosine (AzY), p-azido-L-phenylalanine (AzF), or a combination thereof;
(b) subsequently exposing the cell to the one or more noncanonical amino acids, thereby integrating the one or more noncanonical amino acids into the protein or population of proteins, and producing a modified protein or population of modified proteins; and
(c) contacting the modified protein or population of modified proteins with a detectable moiety, thereby producing a labeled protein or population of labeled proteins.