Patent ID: 11952586
Assignee: HOFFMANN-LA ROCHE, INC.
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 9:
10. The method according to claim 1, wherein the co-cultivating of the one or more B-cells is with about 10,000 to about 30,000 EL-4 B5 cells, which have been irradiated with gamma radiation of the dose in the range of about 3 Gy to about 6 Gy, wherein the feeder mix comprises about 0.06 ng/ml IL-1beta, about 0.06 ng/ml TNFalpha, about 1.5 ng/ml IL-2, about 0.3 ng/ml IL-10, about 0.3 ng/ml IL-6, and about 0.25 ng/ml-0.5 ng/ml PMA.