Patent ID: 11946085
Assignee: AMGEN INC.
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 0:
1. A method for controlling Mannose-5 glycoform content of denosumab molecules, wherein said glycoform content is measured as percentage of Mannose-5 glycoforms, and wherein the percentage of Mannose-5 glycoforms in the denosumab molecules is increased as compared to a control value, comprising:
(a) establishing a CHO cell culture in a bioreactor with CHO cells that express denosumab and with a first cell culture medium that does not comprise mannose;
(b) growing the CHO cells in said first culture medium until viable cell density is at least 3×106 cells/mL;
(c) providing the CHO cell culture with a second culture medium, wherein said second culture medium comprises mannose and glucose, and wherein the ratio of mannose concentration to total hexose concentration in the second medium is between 0.1 and 0.4; and
(d) recovering the denosumab molecules from the cell culture.