Patent ID: 11906523
Assignee: VENTANA MEDICAL SYSTEMS, INC.
Field: Measurement (Instruments)
Classification: CPC G  C | IPC C  G

Claim 9:
10. The stained biological sample according to claim 1, wherein the chromogenic moiety is selected from the group consisting of tartrazine, 7-diethylaminocoumarin -3-carboxylic acid, DABSYL, fluorescein, a fluorescein derivative, rhodamine, a rhodamine derivative, Rhodamine Green, Rhodamine Green carboxylic acid, an eosin derivative, eosin, 6-carboxy-2′,4,7,7′-tetrachlorofluorescein (TET), carboxyrhodamine 6G, tetramethylrhodamine, carboxytetramethylrhodamine (TMR), QSY 9, sulforhodamine B, a diarylrhodamine derivative, QSY 7, Texas Red, Fast Green FCF, Malachite Green, QSY 21, and Victoria Blue, wherein said chromogenic moiety is covalently linked to said phenolic moiety by:
(i) an amine-reactive group which is an isothiocyanate, an isocyanate, an acyl azide, an NHS ester, an acid chloride, a sulfonyl chloride, a succinimidyl ester, an aldehyde, a glyoxal, an epoxide, an oxirane, a carbonate, an arylating agent, an imidoester, a carbodiimide, or an anhydride;
(ii) a thiol-reactive group which is a haloacetyl halide, an alkyl halide, a maleimide, an aziridine, an acryloyl derivative, an arylating agent, a thiol-disulfide exchange reagent, a pyridyl disulfide, a TNB-thiol, or a disulfide reductant;
(iii) a carboxylate-reactive group which is a diazoalkane, a diazoacetyl compound, a carbonyldiimidazole compound, or a carbodiimide;
(iv) a hydroxyl-reactive group which is an epoxide, a carbonyldiimidazole, an N,N′-disuccinimidyl carbonate, an N-hydroxysuccinimidyl chloroformate, a periodate oxidizing compound, an alkyl halide, or an isocyanate;
(v) an aldehyde and ketone-reactive group which is a hydrazine, a Schiff base, a reductive amination product, or a Mannich condensation product;
(vi) an active hydrogen-reactive group which is a diazonium derivative, a Mannich condensation product, or an iodination reaction product; or
(vii) a photoreactive group which is an aryl azide, a halogenated aryl azide, a benzophenone, a diazo compound, or a diazirine derivative.