Patent ID: 11873326
Assignee: KOREA ADVANCED INSTITUTE OF SCIENCE AND TECHNOLOGY
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 0:
1. A transgenic CHO cell line wherein a bone morphogenetic protein (BMP) type I receptor gene (BMPRIA) or a BMP type II receptor gene (BMPRII) is knocked out by introducing into the CHO cell line:
a) a single vector comprising a nucleotide sequence of sgRNA represented by SEQ ID NO:6 targeting the 2nd exon nucleotide sequence of the BMPRIA gene represented by SEQ ID NO:1 and a nucleotide sequence encoding Cas9 protein represented by SEQ ID NO: 9; or
b) a single vector comprising a nucleotide sequence of sgRNA represented by SEQ ID NO:7 targeting the 2nd exon nucleotide sequence of the BMPRII gene represented by SEQ ID NO:2, a nucleotide sequence of sgRNA represented by SEQ ID NO: 8 targeting the 5th exon nucleotide sequence of the BMPRII gene represented by SEQ ID NO:5, and a nucleotide sequence encoding Cas9 protein represented by SEQ ID NO:9.