Patent ID: 11931405
Assignee: GLAXOSMITHKLINE BIOLOGICALS SA
Field: Pharmaceuticals (Chemistry)
Classification: CPC A  C  Y | IPC A  C

Claim 13:
14. A recombinant host cell for producing a bioconjugate of an E. coli glucosylated O4 antigen polysaccharide covalently linked to a carrier protein, wherein the glucosylated O4 antigen polysaccharide comprises the structure of Formula, wherein n is an integer of 5 to 40,
the host cell comprising:
(i) a nucleotide sequence of an rfb gene cluster for the E. coli O4 antigen polysaccharide;
(ii) a gtrS gene encoding a glucosyl transferase enzyme specific for E. coli serotype O4 that glucosylates the O4 antigen polysaccharide;
(iii) a grtA gene encoding a bactoprenol-linked glucose translocase;
(iv) a gtrB gene encoding a bactoprenol glucosyl transferase;
(v) a nucleotide sequence encoding the carrier protein; and
(vi) a nucleotide sequence encoding an oligosaccharyl transferase capable of covalently linking the E. coli glucosylated O4 antigen polysaccharide to the carrier protein to produce the bioconjugate.