Patent ID: 11946067
Assignee: IFP ENERGIES NOUVELLES
Field: Biotechnology (Chemistry)
Classification: CPC C  Y | IPC C

Claim 11:
12. The process according to claim 11, characterized in that the process further comprises one or more additional steps (d), subsequent to step (b) or to step (c), comprising
(i) introducing an nth nucleic acid containing a repair template distinct from that already introduced and one or more expression cassettes for guide RNAs allowing the integration of an nth sequence of interest contained in said distinct repair template into a targeted region of the bacterium's genome, in the presence of the inducer of expression of the anti-CRISPR protein,
(ii) culturing the bacterium thus transformed on a medium not containing the inducer of expression of the anti-CRISPR protein, and
(iii) allowing expression of a Cas9/gRNA ribonucleoprotein complex.