Patent ID: 11952401
Assignee: LANZHOU VETERINARY RESEARCH INSTITUTE, CHINESE ACADEMY OF AGRICULTURAL SCIENCES
Field: Pharmaceuticals (Chemistry)
Classification: CPC C  A | IPC A  C

Claim 3:
4. A method for preparing a recombinant FMDV, comprising the following steps:
(1) constructing a full-length infectious clone of an FMDV;
(2) mutating the amino acids at position 17 for each of 3B1, 3B2, and 3B3 of a 3B protein to a polar amino acids using a gene mutation technology or a gene synthesis technology to obtain a recombinant plasmid;
(3) transfecting FMDV-sensitive cells with the recombinant plasmid, and culturing the transfected FMDV-sensitive cells to obtain the recombinant FMDV.