Patent ID: 11927523
Assignee: BECKMAN COULTER, INC.
Field: Measurement (Instruments)
Classification: CPC G | IPC G

Claim 0:
1. A method of flow cytometry comprising the steps of:
establishing a fluid stream;
entraining particles in said fluid stream;
detecting a first signal affiliated with individual particles in said fluid stream;
detecting at least one additional signal affiliated with said individual particles;
converting said first signal and said at least one additional signal affiliated with said individual particles into n-dimensional parameter data;
providing an n-dimensional space alteration function having alteration values based upon an angle of rotation to increase discrimination of at least two populations of said n-dimensional parameter data;
combining said n-dimensional parameter data with the n-dimensional space alteration function to mathematically achieve numerical results representative of a rotational alteration;
distinguishing said at least two populations of said n-dimensional parameter data;
real-time classifying said n-dimensional parameter data of each of said individual particles into one of said at least two populations based upon said numerical results representative of said rotational alteration; and
sorting said individual particles based upon said real-time classification.