Patent ID: 11955241
Assignee: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA
Field: Measurement (Instruments)
Classification: CPC G  Y | IPC G

Claim 0:
1. A method comprising:
obtaining a skin tissue biopsy sample(s) and/or extracellular vesicle (EV) serum sample(s) from a subject suspected of having or having a vascular anomaly or malformation selected from the group consisting of arterio-venous malformations (AVM), venous malformations (VM), port-wine stain (PWS), Sturge-Weber syndrome (SWS), Klippel-Trenaunay-Weber syndrome (KTWS), hemangioma, cavernoma, capillary telangiectasia, Dural AV fistula, and angiokeratoma;
measuring the expression profile of a set of pathogenic associated biomarkers and/or serum EV biomarkers from the sample(s) obtained from the subject,
wherein the set of pathogenic associated biomarkers and/or serum EV biomarkers comprises VAT1, IQGAP1, HSC70, CLH1, perlecan, spectrin α1, and GDIR1;

comparing the expression profile of the set pathogenic associated biomarkers and/or serum EV biomarkers from the subject's skin tissue biopsy sample(s) and/or EV serum sample(s) with the expression profile of the same set pathogenic associated biomarkers and/or serum EV biomarkers from control skin tissue biopsy sample(s) and/or EV serum sample(s) that do not have the vascular anomaly or malformation; and
treating the subject with a pulsed dye laser or intense pulsed light, based upon a significant difference in the protein expression profile of the set biomarkers from the subject samples in comparison with control skin tissue biopsy sample(s) and/or EV serum sample(s).