Patent ID: 11959117
Assignee: NATURE'S TOOLBOX, INC.
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 0:
1. A system for the production of pseudouridine compounds comprising:
an isolated uracil nucleobase, and isolated ribose-5-phosphate, wherein the uracil nucleobase with the ribose-5-phosphate are converted to form pseudouridine-5′-monophosphate (ΨMP), wherein the formation of ΨMP is catalyzed by pseudouridine-5′-phosphate glycosidase, or a functional fragment or variant thereof;
a protecting agent that reacts with the hydroxyl groups of said ΨMP forming a protected ΨMP;
a methylating agent that reacts with the protected ΨMP, forming a protected N1-methyl -pseudouridine-5′-monophosphate (protected m1ΨMP) compound;
a deprotection agent that reacts with the protected m1ΨMP forming N1-methyl-pseudouridine-5′-monophosphate (m1ΨMP); and
a phosphorylating agent that catalyzes the sequential phosphorylation of m1MP forming N1-methyl-pseudouridine-5′-diphosphate (m1ΨTP), and N1-methyl-pseudouridine-5′-triphosphate (m1ΨTP).