Patent ID: 11866470
Assignee: INDUSTRY-UNIVERSITY COOPERATION FOUNDATION HANYANG UNIVERSITY
Field: Biotechnology (Chemistry)
Classification: CPC C  A | IPC A  C

Claim 0:
1. A triblock polypeptide having multi-stimuli responsiveness, wherein the triblock polypeptide is consisted of
a calmodulin block; and
polypeptide blocks having a phase transition behavior linked to both ends of the calmodulin block, and is represented by Formula 4 below:

[hydrophobic EBP]m-[hydrophilic EBP]x-Cal[hydrophilic EBP]x-[hydrophobic EBP]m, wherein  Formula 4

the [hydrophobic EBP]m refers to a polypeptide block having a phase transition behavior;
the [hydrophobic EBP]m-[hydrophilic EBP]x refers to a polypeptide block having a phase transition behavior;
the hydrophobic EBP is consisted of a hydrophobic polypeptide which is a pentapeptide of Val-Pro-Ala-Xaa-Gly (SEQ ID NO: 2);
the hydrophilic EBP is consisted of a hydrophilic polypeptide which is a pentapeptide of Val-Pro-Ala-Xaa-Gly (SEQ ID NO: 2);
wherein Xaa is a natural amino acid other than proline;
m or x, each respectively, is an integer of 2 or more, and is the number of repeats of the hydrophobic polypeptide and hydrophilic polypepteide having a phase transition behavior;
the CalM is a calmodulin block, wherein an amino acid sequence encoding the calmodulin is SEQ ID NO: 38,
wherein each Xaa of the pentapeptide repeats is consisted of A(Ala), G(Gly), I(Ile); K(Lys), G(Gly), I(Ile); D(Asp), G(Gly), I(Ile); and E(Glu), G(Gly), I(Ile) in a ratio of 1:4:1;
each Xaa of the pentapeptide repeats is consisted of G(Gly), A(Ala), F(Phe); K(Lys), A(Ala), F(Phe); and D(Asp), A(Ala), F(Phe) in a ratio of 1:3:2;
each Xaa of the pentapeptide repeats is consisted of K(Lys) and F(Phe) in a ratio of 3:3;
each Xaa of the pentapeptide repeats is consisted of D(Asp) and F(Phe) in a ratio of 3:3;
each Xaa of the pentapeptide repeats is consisted of H(His), A(Ala), and I(Ile) in a ratio of 3:2:1;
each Xaa of the pentapeptide repeats is consisted of H(His) and G(Gly) in a ratio of 5:1; or
each Xaa of the pentapeptide repeats is consisted of G(Gly), C(Cys), and F(Phe) in a ratio of 1:3:2;
wherein the polypeptide block is further linked a cysteine block with both end of thereof;
wherein the cysteine block is consisted of an amino acid sequence in which (Gly-Ala-Cys) repeats one or more times.