Patent ID: 11926867
Assignee: 10X GENOMICS, INC.
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 2:
3. The method of claim 2, wherein the amplifying comprises:
(a) hybridizing the capture domain of the capture probe to the sequence complementary to the capture domain of the second primer;
(b) extending the second primer using the capture probe as a template to generate a copy of the template oligonucleotide immobilized on the substrate;
(c) releasing the copy of the template oligonucleotide from the capture probe;
(d) hybridizing the copy of the template oligonucleotide to an unextended first primer; and
(e) extending the unextended first primer using the copy of template oligonucleotide as a template to generate a second capture probe immobilized on the substrate, thereby generating the clonal cluster of capture probes.