Patent ID: 11970708
Assignee: ARTICURE INC.
Field: Pharmaceuticals (Chemistry)
Classification: CPC C  A | IPC A  C

Claim 12:
13. A method for preparing a split-dual replicating-retrovirus vector system with minimized recombination occurrence comprising the following steps:
1) a step of preparing a first recombinant retroviral expression vector containing a Gag-Pol gene of MuLV (Murine Leukemia virus), a sEF1α promoter or a first MCMV promoter, and operably linked to a first therapeutic gene, wherein the first MCMV promoter is a minimized promoter consisting of a sequence selected from SEQ ID NO: 4, NO: 5, NO: 6 and NO: 7; and
2) a step of preparing a second recombinant retroviral expression vector containing an Env gene of a virus, and a second MCMV promoter, and operably linked to a second therapeutic gene, wherein the second MCMV promoter is a minimized promoter consisting of a sequence selected from SEQ ID NO: 4, NO: 5, NO: 6 and NO: 7.