Patent ID: 11873326
Assignee: KOREA ADVANCED INSTITUTE OF SCIENCE AND TECHNOLOGY
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 3:
4. A method for preparing a transgenic CHO cell line, wherein the BMP receptor gene BMPRIA or BMPRII is knocked-out, the method comprising the following steps:
1) constructing a vector to knock-out the BMP receptor gene BMPRIA or BMPRII;
2) introducing the vector of step 1) into a CHO cell line, wherein the vector is
a) a nucleotide sequence of sgRNA represented by SEQ ID NO: 6 targeting the 2nd exon nucleotide sequence of BMPRIA gene represented by SEQ ID NO: 1 and a nucleotide sequence encoding Cas9 protein represented by SEQ ID NO: 9; or
b) a nucleotide sequence of sgRNA represented by SEQ ID NO: 7 targeting the 2nd exon nucleotide sequence of BMPRII gene represented by SEQ ID NO: 2, a nucleotide sequence of sgRNA represented by SEQ ID NO: 8 targeting the 5th exon nucleotide sequence of BMPRII gene represented by SEQ ID NO: 5 and a nucleotide sequence encoding Cas9 protein represented by SEQ ID NO: 9; and
3) selecting the cell line in which the BMP receptor gene BMPRIA or BMPRII has been knocked-out from those CHO cell lines introduced with the vector of step 2).