Patent ID: 11908130
Assignee: PROTEIN METRICS, LLC
Field: Measurement (Instruments)
Classification: CPC G | IPC G

Claim 9:
10. A method for generating image data for a set of stained slides that comprises a tissue sample, the method comprising:
selecting a plurality of sub-regions from one of the set of stained slides, wherein at least one of the sub-regions includes a structure that is substantially free of cells;
identifying, for each imaging channel of a first plurality of imaging channels: a first number of variations of contrast thresholds, a first number of variations of thresholds of marker positivity, or a first number of variations of contrast thresholds and a first number of variations of thresholds of marker positivity, wherein each imaging channel is associated with a corresponding biomarker or plurality of biomarkers;
generating a plurality of masks comprising a mask for each of the plurality of sub-regions corresponding to each combination of the variations of contrast thresholds and the variations of thresholds of marker positivity for each imaging channel of the first plurality of imaging channels, wherein each mask is segmented to identify cells using the variations of contrast thresholds and the variations of thresholds of marker positivity;
ranking the plurality of masks based on an ability of each of the plurality of masks to accurately identify cells that are positive for the corresponding biomarker or plurality of biomarkers of each imaging channel of the first plurality of imaging channels;
selecting a set of final mask parameters from the plurality of masks based on the rankings; and
generating a set of slide images for all slides of the set of stained slides using the set of final mask parameters.