Patent ID: 11921274
Assignee: CARL ZEISS MICROSCOPY GMBH
Field: Measurement (Instruments)
Classification: CPC G | IPC G

Claim 0:
1. A method for detecting emission light in a laser scanning microscope, in which emission light coming from a sample is guided by way of an imaging optical unit to a two-dimensional matrix sensor that is situated in an image plane and has a multiplicity of pixels, the method comprising:
detecting a detection point spread function, wherein the detecting includes spatially oversampling the detection point spread function using the matrix sensor,
spectrally separating the emission light coming from the sample using a dispersion device,
separately detecting the spectrally separated emission light using the matrix sensor, and evaluating intensities measured by the pixels of a pixel region of the detection point spread function, wherein the evaluating includes reversing the spectral separation for at least some of these pixels,
wherein the reversing the spectral separation for the at least some pixels of a pixel region includes assigning intensity values measured by the pixels to a location in the image plane that has been displaced relative to the respective pixel, using a displacement vector depending on the location of the respective pixel and a wavelength associated with that location.