Patent ID: 11898139
Assignee: OKAYAMA PREFECTURAL GOVERNMENT
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 7:
8. A method for inhibiting an increase in viscosity of a culture solution of Rhizobium bacteria,
the method comprising culturing a Rhizobium bacterium by aeration-agitation culture using a medium comprising one or more carbon sources, wherein a disaccharide accounts for at least 25 mass % of the one or more carbon sources in the medium while maintaining a pH of 5.0 to 7.0 in a culture solution from a beginning of the culturing to an end of the culturing,
wherein the disaccharide is at least one selected from the group consisting of maltose, trehalose, and lactose, and
wherein the Rhizobium bacterium is a Rhzizobium vitis ARK-1 strain with accession number FERM BP-11426, a Rhizobium vitis ARK-2 strain with accession number FERM BP-11427, a Rhizobium vitis ARK-3 strain with accession number FERMI BP-11428, or an Agrobacterium radiobacter K84 strain,
whereby, at the time of culturing a Rhizobium bacterium, and, at the time of isolating and recovering a viable cell thereof, the viscosity of culture solution of Rhizobium bacteria is maintained by inhibiting the increase in viscosity.