Patent ID: 11921123
Assignee: WUHAN UNIVERSITY OF TECHNOLOGY
Field: Measurement (Instruments)
Classification: CPC G | IPC G

Claim 2:
3. The method according to claim 1, wherein in the step (b),
said separately treating the at least one test biological tissue sample of the group to be tested, and the at least one control biological tissue sample of the blank control group with the acid solution comprises:
measuring a mass or volume of the at least one test biological tissue sample in the group to be tested and a mass or volume of the at least one control biological tissue sample in the blank control group separately;
adding the acid solution to the at least one test biological tissue sample in the group to be tested and the at least one control biological tissue sample in the blank control group separately to obtain the homogenates of the group to be tested and the blank control group;
taking an amount of the homogenate in the group to be tested and an amount of the homogenate in the blank control group, wherein the amount of the homogenate in the group to be tested and the amount of the homogenate in the blank control group are equivalent;

said diluting the supernatants of the group to be tested and the blank control group with the fluorescent enhancement liquid comprises:
mixing the supernatants of the group to be tested and the blank control group with the acid solution according to a proportion to obtain supernatant diluents of the group to be tested and the blank control group, respectively;
mixing the supernatant diluents of the group to be tested and the blank control group with the fluorescent enhancement liquid according to a proportion to obtain mixed liquids of the group to be tested and the blank control group, respectively; and

said performing the significant difference analysis on the values T and T0 of the fluorescent intensities per unit mass or volume between the at least one test biological tissue sample of the group to be tested and the at least one control biological tissue sample of the blank control group comprises:
when P is greater than or equal to 0.05, indicating that significant difference does not exist between the at least one test biological tissue sample in the group to be tested and the at least one control biological tissue sample in the blank control group, and determining that the at least one test biological tissue sample in the group to be tested does not contain the RE-nCaP; and
when P is smaller than 0.05, indicating that the significant difference exists between the at least one test biological tissue sample in the group to be tested and the at least one control biological tissue sample in the blank control group, determining that the at least one test biological tissue sample in the group to be tested contains the RE-nCaP.