Patent ID: 11959084
Assignee: AUTOLUS LIMITED
Field: Pharmaceuticals (Chemistry)
Classification: CPC C  A | IPC C

Claim 2:
3. A nucleic acid construct according to claim 1 which has the structure:
NOI1-TRM-CL-NOI2,
NOI1-TRM1-TRM2-CL-NOI2,
NOI1-CL1-TRM-CL2-NOI2,
NOI1-TRM-CL-SP-NOI2,
NOI1-TRM1-CL1-NOI2-TRM2-CL2-NOI3, or
NOI1-TRM1-CL1-TRM2-CL2-NOI2,
in which:
TRM1 and TRM2, which may be the same or different, are first and second translational readthrough motifs;
CL1 and CL2, which may be the same or different, are first and second nucleic acid sequences each encoding a cleavage site,
SP is an attenuated signal peptide, and
NOI3 is a third nucleotide sequence of interest.