Patent ID: 11891600
Assignee: ILLUMINA, INC.
Field: Biotechnology (Chemistry)
Classification: CPC C  G | IPC C  G

Claim 16:
17. A sample-indexed nucleic acid library preparation, comprising:
a first nucleic acid library prepared from a first sample, wherein the first nucleic acid library comprises a first plurality of dual-indexed nucleic acid fragments having respective different target sequences, wherein the first nucleic acid library comprises a first sample 5′ set of different 5′ index sequences and a first sample 3′ set of different 3′ index sequences, and wherein each nucleic acid fragment of the first plurality comprises a 5′ index sequence selected from the first sample 5′ set and a 3′ index selected from the first sample 3′ set, and wherein the different 5′ index sequences and the different 3′ index sequences are randomly combined among the first plurality of dual-indexed nucleic acid fragments such that a plurality of possible combinations of the 5′ index sequence and the 3′ index sequence are represented in the first nucleic acid library and wherein at least one possible combination is present in more than one fragment of the first nucleic acid library; and
a second nucleic acid library prepared from a second sample, wherein the second nucleic acid library comprises a second plurality of dual-indexed nucleic acid fragments having respective different target sequences, wherein the second nucleic acid library comprises a second sample 5′ set of different 5′ index sequences and a second sample 3′ set of different 3′ index sequences, and wherein each nucleic acid fragment of the second plurality comprises a 5′ index sequence selected from the second sample 5′ set and a 3′ index selected from the second sample 3′ set, and wherein the different 5′ index sequences and the different 3′ index sequences are randomly combined among the second plurality of dual-indexed nucleic acid fragments such that a plurality of possible combinations of the 5′ index sequence and the 3′ index sequence are represented in the second nucleic acid library, wherein at least one possible combination is present in more than one fragment of the second nucleic acid library, and wherein the first sample 5′ set of different 5′ index sequences, the first sample 3′ set of different 3′ index sequences, the second sample 5′ set of different 5′ index sequences and the second sample 3′ set of different 3′ index sequences are all distinguishable from one another.