Patent ID: 11859210
Assignee: SCRIPPS HEALTH
Field: Pharmaceuticals (Chemistry)
Classification: CPC C  A | IPC A  C

Claim 0:
1. A method of regenerating cartilaginous tissue, the method comprising:
a. culturing pluripotent cells directly on tissue culture-grade plastic;
b. passaging and culturing the pluripotent cells at least two times to generate a population of cells expressing at least three proteins selected from CD90, CD44, CD105, and CD73;
c. culturing the population of cells expressing at least three proteins selected from CD90, CD44, CD105, and CD73 in a three-dimensional culture to generate chondrogenic precursor cells; and
d. transplanting the chondrogenic precursor cells into a bone or cartilage defect, wherein a regenerated cartilaginous tissue is produced in the bone or cartilage defect.