Patent ID: 11860118
Assignee: BOARD OF REGENTS, THE UNIVERSITY OF TEXAS SYSTEM
Field: Measurement (Instruments)
Classification: CPC G  B | IPC B  G

Claim 15:
16. An aggregation-induced quantitative photothermal biosensing method of detecting a target, the method comprising:
irradiating a biosensor containing a sample with near-infrared radiation for a selected time, wherein the biosensor comprises a suspension of a hybridization buffer containing gold nanoparticles, sodium chloride, and single-stranded DNA probes configured to undergo DNA hybridization with a target, and wherein the near-infrared radiation is configured to induce a photothermal effect in any aggregated gold nanoparticles present in the biosensor;
determining a temperature change of the biosensor after irradiating the biosensor with the near-infrared radiation for the selected time;
determining a concentration of the target present in the sample using the temperature change of the biosensor; and
irradiating the suspension at a power density of 5.2 W/cm2 for an irradiation time, wherein the irradiation time is from about 5 minutes to about 10 minutes.