Patent ID: 11970551
Assignee: WNTRESEARCH AB
Field: Biotechnology (Chemistry)
Classification: CPC C  Y | IPC C

Claim 0:
1. A solution phase method for preparing PG1-Met-Asp(OtBu)-Gly-Cys(Trt)-Glu(OtBu)-Leu-OtBu, the method comprising:
a. Sequentially coupling tripeptide Fmoc-Cys(Trt)Glu(OtBu)-Leu-OtBu (KT-2*) with Fmoc-Gly-OH, Fmoc-Asp-OtBu and PG1-Met, or
b. Sequentially coupling tetrapeptide Fmoc-Gly-Cys(Trt)-Glu(OtBu)-Leu-OtBu (SEQ_ID NO 6) with Fmoc-Asp-OtBu and PG1-Met,
wherein
i. Fmoc deprotection is achieved with DBU, and wherein
ii. DBU is removed from the reaction mass before coupling with Fmoc-Asp-OtBu, and wherein
iii. PG1 is a base sensitive protecting group.