Patent ID: 11965158
Assignee: ILLUMINA, INC.
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 0:
1. A system for obtaining captured contiguity information from a double-stranded target nucleic acid comprising:
a plurality of transposomes, wherein each transposome comprises a transposase and a transposon nucleic acid, wherein the transposase comprises an activity to nick only one strand of the double-stranded target nucleic acid;
the double-stranded target nucleic acid, wherein the target nucleic acid is nicked at a plurality of sites and single transposon nucleic acids are attached to at least one of the nicked sites; and
a plurality of polynucleotide fragments prepared with the plurality of transposomes, wherein the plurality of polynucleotide fragments is immobilized on a surface and comprises overlapping sequences in a linear representation of a single target nucleic acid sequence, and wherein at least one end of the plurality of polynucleotide fragments comprises the transposon nucleic acid.