Patent ID: 11946865
Assignee: BECTON, DICKINSON AND COMPANY
Field: Measurement (Instruments)
Classification: CPC G  A  C | IPC A  C  G

Claim 5:
6. The method of claim 4, further comprising, prior to inoculating the blood culture test vial with the blood sample:
introducing a second reference fluid into a second reference vial comprising a second reference vial sensor comprising a fluorophore and a pH indicator, the second reference fluid exposing the second reference vial sensor in the second reference vial to a second performance boundary condition, wherein the first performance boundary condition is a low pH condition and the second performance boundary condition is a high pH condition;
transmitting light at an excitation frequency of the second reference vial sensor to the second reference vial to cause the second reference vial to emit a second reference vial fluorescence signal;
filtering the second reference vial fluorescence signal using the first emission filter and the second emission filter;
measuring an intensity of the second reference vial fluorescence signal that was filtered using the first emission filter and an intensity of the second reference vial fluorescence signal that was filtered using the second emission filter to generate a second reference ratio.