Patent ID: 11873487
Assignee: UNIVERSITY OF MASSACHUSETTS
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 8:
9. The method of claim 1, wherein the donor sequence is heat denatured and renatured following the steps comprising:
1) Incubating at 95° C. for about 10 seconds to about 10 minutes,
2) incubating at 85° C. for about 5 seconds to about 2 minutes,
3) Incubating at 75° C. for about 5 seconds to about 2 minutes,
4) incubating at 65° C. for about 5 seconds to about 2 minutes,
5) incubating at 55° C. for about 5 seconds to about 2 minutes,
6) incubating at 45° C. for about 5 seconds to about 2 minutes,
7) incubating at 35° C. for about 5 seconds to about 2 minutes, and
8) incubating at 25° C. for about 5 seconds to about 2 minutes.