Patent ID: 11921105
Assignee: nan
Field: Measurement (Instruments)
Classification: CPC C  G | IPC G

Claim 16:
17. A method for identifying a compound that enhances the activity of an olfactory receptor induced by an agonist odorant compound comprising:
(i) screening one or more compounds in a binding assay in the presence of the agonist odorant compound;
(ii) selecting one or more of the compounds screened that specifically enhances the specific binding or the effect of the binding of an agonist odorant compound to a mammalian olfactory receptor; and
(iii) identifying compounds that potentially modulate the perception of the agonist odorant compound based on their enhancement of the specific binding or the effect of the binding of the agonist odorant compound to the olfactory receptor,
wherein the olfactory receptor comprises a polypeptide that
comprises an amino acid sequence having at least 95% sequence identity to SEQ ID NO: 2, 4, 6, or 8; or
is encoded by a nucleic acid molecule comprising a nucleotide sequence having at least 95% sequence identity to SEQ ID NO: 1, 3, 5, or 7, or the reverse complement thereof,
wherein the agonist odorant compound is a musk or a floral muguet compound,
wherein the compound has the structure:, in the form of any one of its stereoisomers or a mixture thereof,
wherein R represents a C3-11 linear alkyl group optionally substituted by a C1-4 alkyl carboxylester group or a hydroxyl group, a C4-11 branched alkyl group optionally substituted by a C1-3 alkoxy group, a C6-12 linear or branched alkenyl or alkadienyl group, a phenyl group substituted by one or two C1-3 alkyl groups, a C5-8 alicyclic alkenyl group or a benzyloxymethyl group, or
wherein the compound is at least one positive allosteric modulator selected from the group consisting of (E)-dec-2-enal, 2-phenylpropanal; (E)-but-2-enal; 3-methylbenzaldehyde; 3-(1,3-benzodioxol-5-yl)-2-methylpropanal; (+−)-3-(4-methyl-3-penten-1-yl)-3-cyclohexene-1-carbaldehyde, (+−)-4-(4-methyl-3-penten-1-yl)-3-cyclohexene-1-carbaldehyde, and mixtures thereof; heptanal, 4-Propan-2-ylbenzaldehyde; (3R)-3,7-dimethyloct-6-enal; (+)-(3S)-3-[(1R)-4-methyl-3-cyclohexen-1-yl]butanal, (+)-(3R)-3-[(1R)-4-methyl-3-cyclohexen-1-yl]butanal, and mixtures thereof, hexanal; 2,6-dimethylhept-5-enal; benzaldehyde; 2-methyl-3-(4-methylphenyl)propanal; 3,5,6-trimethyl-3-cyclohexene-1-carbaldehyde, 2,4,6-trimethyl-3-cyclohexene-1-carbaldehyde, and mixtures thereof, 4-ethylbenzaldehyde; 6-methoxy-2,6-dimethylheptanal; (E)-non-2-enal; and combinations thereof.