Patent ID: 11898195
Assignee: THE UNIVERSITY OF CHICAGO
Field: Measurement (Instruments)
Classification: CPC C  G  A | IPC C  G

Claim 0:
1. A nucleic acid complex comprising:
i) a first single-stranded nucleic acid molecule comprising the nucleic acid sequence:, (SEQ ID NO. 8)

5′-CCCTAACCCCTAAC*CC*CTAACC*CC*ATATATATCCTAGAACGAC

 

AGACAAACAGTGAGTC-3;

 

(SEQ ID NO. 9)

5′-CCC*CTAACC*CCTAACCC*CTAACC*CCATATATATCCTAGAACGA

 

CAGACAAACAGTGAGTC-3′;

 

(SEQ ID NO. 10)

5′-C*CCCTAACCCC*TAAC*CCCTAACCCC*ATATATATCCTAGAACGA

 

CAGACAAACAGTGAGTC-3′;

or

 

(SEQ ID NO. 11)

5′-C*C*C*C*TAACCCCTAACCCCTAACCCCATATATATCCTAGAACGA

 

CAGACAAACAGTGAGTC-3′;

wherein C* is a 5′-bromocytosine or 5′-methylcytosine and wherein the first single-stranded nucleic acid molecule is conjugated to a first fluorophore which comprises a donor fluorophore as a first label; and
ii) a second single-stranded nucleic acid molecule that is partially or fully complementary to the first single-stranded molecule, wherein the second single-stranded nucleic acid molecule comprises the nucleic acid sequence:

5′-GACTCACTGTTTGTCTGTCGTTCTAGGATAT*ATATTTTGTTATGTGTTATGTGTTA T-3′ (SEQ ID NO:19)(P comp) and wherein T* is conjugated to a second fluorophore which comprises an acceptor fluorophore as a second label,
wherein the first and the second labels arc a donor-acceptor pair.