Patent ID: 11867688
Assignee: DANA-FARBER CANCER INSTITUTE, INC.
Field: Measurement (Instruments)
Classification: CPC G | IPC G

Claim 7:
8. A kit comprising:
a multi-well plate for automated drug screening, the multi-well plate comprising an array of at least 384 wells, wherein a plurality of wells in the array are coated with an adhesive agent, wherein at least one well of the plurality comprises a test therapeutic agent and a BH3 domain peptide; and
a vial containing a BH3 profiling buffer at a concentration of 2×, 3×, 4×, or 5×,
wherein the BH3 profiling buffer is a Derived from Trehalose Experimental Buffer (DTEB) at a concentration of 2×, 3×, 4×, or 5×, wherein a 1× concentration of DTEB comprises: 135 mM trehalose, 10 mM HEPES, 50 mM KCl, 0.02 mM EGTA, 0.02 mM EDTA, 0.1% BSA, and 5 mM succinate, or
wherein the BH3 profiling buffer is a Mannitol Experimental Buffer (MEB) at a concentration of 2×, 3×, 4×, or 5×, wherein a 1× concentration of MEB comprises: 150 mM mannitol, 10 mM HEPES, 50 mM KCl, 0.02 mM EGTA, 0.02 mM EDTA, 0.1% BSA, and 5 mM succinate.