Patent ID: 11891595
Assignee: WILSON WOLF MANUFACTURING
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 23:
24. A method of assessing functional characteristics of T cells comprising:
Adding cancer cells into a matrix bio scaffold that is attached to the bottom of a compartment of a multi-compartment cell culture apparatus comprising: at least a first compartment, a second compartment, and a third compartment, each compartment separated from adjacent compartments by a common wall, wherein the wall is mated to a bottom of the compartments, and adapted to hold a volume of medium at a medium volume to bottom footprint ratio of at least 2, each compartment including at least one permanently open passage, wherein the permanently open passage is an opening through the common wall, connecting the bottom of adjacent compartments that allows the contents of one compartment to communicate with another, the bottom of the compartments being gas permeable, the bottom of the compartments and the bottom of the permanently open passages being flat in a common horizontal plane, and not including medium mixing equipment, medium perfusion equipment, or gas pumping equipment;
adding a volume of medium into the multi-compartment cell culture apparatus, allowing an engraftment period for the cancer cells to engraft into said matrix bio scaffold;
adding T-Cells into a compartment that differs from the compartment that the matrix bio scaffold is attached, allowing a period of time for the T cells to follow a chemokine gradient expressed by the cancer cells; and
measuring the presence of the T Cells in the compartment containing the matrix bio scaffold.