Patent ID: 11939637
Assignee: THE GENERAL HOSPITAL CORPORATION
Field: Measurement (Instruments)
Classification: CPC C  G | IPC C  G

Claim 0:
1. A method of treating cancer in a patient in need thereof, comprising
(a) selecting a patient suffering from cancer for immunotherapy by detecting cytolytic activity in tumor tissue from the patient, wherein detecting cytolytic activity comprises measuring cytolytic activity in tumor tissue from the patient by assaying the expression of granzyme A (GZMA) and perforin (PRF1), wherein the measuring comprises sequencing RNA expressed in the tumor tissue, and comprises (i) calculating the log-average of the transcript levels of granzyme A (GZMA) and perforin (PRF1), and (ii) assigning a cytolytic activity (CYT) score to the tumor based on the log average calculated in (i); and
(b) administering to the patient an agent that stimulates the patient's preexisting immune response if the cytolytic activity detected in the tumor is at least two-fold greater than the median value observed among patients diagnosed with the same histological cancer type.