Patent ID: 11932858
Assignee: nan
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 18:
19. The library of claim 15, wherein the library comprises two or more homologous recombination constructs, wherein each construct comprises:
a. a nucleic acid cassette specific for a distinct nucleic acid locus and comprising a nucleic acid expression construct encoding a gRNA of a CRISPR-based nucleic acid modification system specific for the nucleic acid locus, a nucleic acid expression construct encoding a gRNA of a CRISPR-based transcription activation system, and a donor polynucleotide encoding a reporter flanked by regions homologous to the nucleic acid locus; and
b. a modular homologous recombination construct comprising a backbone encoding additional components of the CRISPR-based nucleic acid modification system and the CRISPR-based transcription activation system.