Patent ID: 11964997
Assignee: REALSEQ BIOSCIENCES, INC.
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 3:
4. The method of claim 1, comprising ligating the combo adapter to the 5′ end of said plurality of sample polynucleotides wherein (a) said plurality of combo adapters comprising 5′-p and 3′-p ends is ligated to a 5′-OH end of a sample polynucleotide having a 3′-OH end or a 2′,3′>p end, or (b) said plurality of combo adapters comprises 5′-OH and 3′-OH ends, and is ligated to a 5′-p end of a sample polynucleotide comprising a phosphorylated 3′-end selected from 2′,3′>p, 3′-p, or 2′-p.