Patent ID: 11911411
Assignee: GLYCOLOGIX, INC.
Field: Pharmaceuticals (Chemistry)
Classification: CPC A  C | IPC A  C

Claim 0:
1. A method of preparing a water-soluble sulfated GAG conjugate, comprising the steps of:
i) contacting a sulfated GAG in aqueous solution at a concentration of 2 wt %-20 wt % with an effective amount of linking agent, wherein the molar ratio of GAG hydroxyl groups to linking agent is less than that required for gel formation, under conditions sufficient to react the linking agent with the sulfated GAG to form a solution comprising a soluble polymer characterized by pendant linking groups;
ii) adding additional sulfated GAG to the solution of step (i) in an amount sufficient to react with a portion of the pendant linking groups to form a soluble polymer conjugate with a branched architecture characterized by remaining pendant linking groups; and
iii) adding a modifier characterized by a reactive moiety under conditions sufficient to react the remaining pendant linking groups with the reactive modifier to form a water-soluble sulfated GAG branched conjugate bearing the modifier.