Patent ID: 11898186
Assignee: GENSCRIPT USA INC.
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC A  C

Claim 4:
5. The method of claim 1, wherein the reaction mixture comprises
a buffer substance in a concentration of from about 45 mM to about 55 mM,
an RNase inhibitor in a concentration of from about 0.01 U/μl to about 0.03 U/μl,
NTPs in a concentration of from about 3 mM to about 5 mM,
the cap analogue in a concentration of from about 6 mM to about 8 mM,
one or more magnesium salts in a concentration of from about 20 mM to about 30 mM,
a polyamine in a concentration of from about 1.5 mM to about 2.5 mM,
the DNA template in a concentration of from about 0.01 μg/μl to about 0.05 μg/μl,
a pyrophosphatase in a concentration of from about 0.1 mU/μl to about 0.5 mU/μl, and
an RNA polymerase in a concentration of from about 0.01 μg/μl to about 0.05 μg/μl.