Patent ID: 11895960
Assignee: KWS SAAT SE & CO. KGAA
Field: Biotechnology (Chemistry)
Classification: CPC A  C  Y | IPC A  C

Claim 0:
1. A plant having biological activity of a haploid inducer and comprising a nucleotide sequence encoding a centromere histone H3 (CENH3) protein comprising a CATD domain, wherein the nucleotide sequence comprises a mutation encoding a polypeptide having one or more amino acid substitutions at positions 2, 6, and 12 of a loop1 consensus sequence, wherein said alteration confers the biological activity of a haploid inducer, wherein the plant is homozygous with respect to the mutation and is not able to express a native CENH3 protein,
wherein the loop1 consensus sequence comprises: T, S, or A at position 1: H, Q, N, A, Y, F, G, D or E at position 2; M, Q, I, F, Y, A, E, N, R, L, H or G at position 3; L, F, V, I or Y at position 4; A, T, S, C or M at position 5; P, N, D, R, A, T, F, R, H, S or K at position 6; Q, Y, D, K, R, E, G, S, P, H, N or A at position 8; I, V or P at position 9; N, G, T, E or S at position 10; R or P at position 11; W or Y at position 12; and T, Q or S at position 13, and
wherein the plant is selected from the group consisting of Sorghum bicolor, Saccharum officinarium, Zea mays, Arabidopsis thaliana, Brassica rapa, and Brassica juncea.