Patent ID: 11913030
Assignee: NATIONAL TAIWAN UNIVERSITY
Field: Biotechnology (Chemistry)
Classification: CPC C  B | IPC C

Claim 0:
1. A method of preparing a mimicking angiogenic co-spheroid, comprising:
(a) co-culturing a neural related cell and a cultured cell on a substrate comprising chitosan to form a co-spheroid of the neural related cell/the cultured cell; and
(b) mixing the co-spheroid of the neural related cell/the cultured cell with a hydrogel to form the mimicking angiogenic co-spheroid, wherein the hydrogel is in the form of a solution or a suspension when the hydrogel is mixed with the co-spheroid of the neural related cell/the cultured cell;
wherein the mimicking angiogenic co-spheroid has stemness, differentiation and angiogenesis ability; the neural related cell is a neural stem cell or a cancer cell; the cultured cell is an endothelial cell or an endothelial progenitor cell;
wherein the hydrogel further comprises a growth factor, and the growth factor is fibroblast growth factor 2 (FGF2);
wherein the hydrogel is a gelatin-based hydrogel;
wherein a capillary-like network is formed on a surface of the mimicking angiogenic co- spheroid encapsulated in the gelatin-based hydrogel after the FGF2 induces growth of the mimicking angiogenic co-spheroid;
wherein the gelatin-based hydrogel is allowed for gelation, and the gelation is performed within five minutes;
wherein the gelatin-based hydrogel is 1-5% gelatin-3,4-hydroxyphenyl-propionic acid (HPA) conjugate;
wherein the substrate comprising chitosan is a hyaluronan-grafted chitosan substrate, and the hyaluronan has a molecular weight ranging from 1500-2000 kDa; and
wherein the mimicking angiogenic co-spheroid enhances cell-cell interactions between the neural related cell and the cultured cell.