Patent ID: 11879027
Assignee: MIPSALUS APS
Field: Pharmaceuticals (Chemistry)
Classification: CPC C  A  B | IPC A  B  C

Claim 2:
3. The MIP according to claim 1, which is obtained by a method comprising the steps of
a) polymerization of a mixture comprising
2-methylprop-2-enoic acid (MAA),
1,4-bis(acryloyl)piperazine (DAP), and
a template molecule consisting of L-Phe or a L-Phe derivative exposing a phenylalanine motif in the presence of a catalyst and an oxidizing agent,

so as to obtain a cross-linked imprinted polymer,
b) if necessary subsequently fragmenting the cross-linked imprinted polymer to obtain a first fragmented polymer, and collecting the MIPs having particle sizes smaller than 63 μm,
c) optionally washing and drying the polymer fraction obtained from step b),
d) fragmenting the polymer fraction obtained from step b) or c) and collecting a second fragmented polymer having particle sizes in the range 150-250 nm,
e) subjecting the second fragmented polymer obtained from step d) to affinity chromatography where Phe constitutes the affinity tag in a chromatographic matrix, and
f) recovering MIPs binding to Phe in step e).