Patent ID: 11857643
Assignee: LIFE TECHNOLOGIES CORPORATION
Field: Measurement (Instruments)
Classification: CPC A  G | IPC A  G

Claim 0:
1. A method for increasing fluorescence of a first fluorescently labeled antibody, the method comprising:
(a) conjugating two or more spacer molecules to a first antibody; and
(b) conjugating from two to ten fluorescent labels to the first antibody;
wherein steps (a) and (b) are done simultaneously or sequentially in any order to form the first fluorescently labeled antibody;
wherein the spacer molecules and fluorescent labels are not conjugated to each other;
wherein the first fluorescently labeled antibody has a fluorescence that is greater than a fluorescence of a second fluorescently labeled antibody that comprises a second antibody that is the same as the first antibody and has the same number and type of fluorescent labels conjugated thereto, but does not include the spacer molecules conjugated to the second antibody; and
wherein the spacer molecules are selected from sulfo-NHS-acetate, MS(PEG)4, or NHS-Betaine.