Patent ID: 11970694
Assignee: PEPTIDREAM INC.
Field: Organic fine chemistry (Chemistry)
Classification: CPC C | IPC C

Claim 0:
1. A process for preparing an [mRNA]-[linker]-[peptide] conjugate in which an mRNA and a peptide as a translation product thereof are coupled via a linker in a reconstituted in vitro protein synthesis reaction solution, which comprises
hybridizing, in the reconstituted in vitro protein synthesis reaction solution, the linker to the mRNA without first ligating the linker to the mRNA; and
translating, in the reconstituted in vitro protein synthesis reaction solution, the mRNA without first ligating the linker to the mRNA,
wherein said mRNA comprises a sequence encoding the peptide sequence and 3′ end region downstream thereof, and
wherein said linker comprises
a chimeric oligonucleotide consisting of a single-stranded structure region that hybridizes with the 3′-end region of the mRNA sequence; said single-stranded structure region is attached at its 3′-end to the 5′ end of an oligo RNA having the sequence 5′-ACCA-3′ via a polyethylene glycol moiety; and
an amino acid attached to the 3′-0 of ribose of the 3′-terminal adenosine of the oligo RNA via an ester bond.