Patent ID: 11884971
Assignee: PACIFIC BIOSCIENCES OF CALIFORNIA, INC.
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 15:
16. A method of characterizing a nucleic acid, comprising:
(a) extending a primer-template nucleic acid hybrid with a polymerase and a mixture of nucleotide cognates of first, second, third and fourth different base types to produce an extended primer hybrid, wherein the nucleotide cognate of the first base type comprises a reversible terminator, wherein at least one of the nucleotide cognates of the second, third or fourth base types is extendable;
(b) contacting the extended primer hybrid with at least one nucleotide cognate to form a stabilized ternary complex comprising a nucleotide cognate of the next base in the template;
(c) detecting the stabilized ternary complex to distinguish the next base, thereby determining the presence of a base multiplet in the template nucleic acid comprising the first base type followed by the next base.