Patent ID: 11873499
Assignee: INSTITUTE OF GENETICS AND DEVELOPMENTAL BIOLOGY, CHINESE ACADEMY OF SCIENCES
Field: Biotechnology (Chemistry)
Classification: CPC C  A  Y | IPC C

Claim 3:
4. A recombinant nucleic acid construct comprising:
a nucleic acid sequence encoding at least one protospacer element, wherein the sequence of the protospacer element is selected from SEQ ID NOs: 50, 53, 58, 61, 66, 69, 74, 77, 82, 86, 91, 94, 99, 102, 107, 110, 115, 118, 123, 126, 131, 134, 139, 142, 147, 150, 155, 158, 163, 166, 171, 174, 179, 182, 187 and 190, and
a nucleic acid sequence encoding at least one tracrRNA sequence, wherein the sequence of the tracrRNA is SEQ ID NO: 46; and/or
wherein the recombinant nucleic acid construct comprises at least one DNA donor nucleic acid selected from SEQ ID NOs: 48, 56, 64, 72, 80, 84, 89, 97, 105, 113, 121, 129, 137, 145, 153, 161, 169, 177 and 185.