Patent ID: 11891651
Assignee: ELEMENT BIOSCIENCES, INC.
Field: Measurement (Instruments)
Classification: CPC C  G | IPC C  G

Claim 13:
14. The method of claim 1, comprising prior to (a):
i) contacting in-solution a plurality of single-stranded circular nucleic acid library molecules with a plurality of first soluble amplification primers, a plurality of a strand displacing polymerases, and a plurality of nucleotides which include dATP, dCTP, dGTP, dTTP and nucleotides having a scissile moiety that can be cleaved to generate an abasic site, under conditions suitable to form a plurality of library-primer duplexes and conduct a rolling circle amplification reaction, thereby generating a plurality of single stranded nucleic acid concatemers, wherein one or more individual single stranded nucleic acid concatemers within the plurality have at least one nucleotide with a scissile moiety;
ii) distributing the rolling circle amplification reaction onto a support having a plurality of the first surface primers immobilized thereon, under conditions suitable for hybridizing one or more portions of individual single stranded concatemers to one or more immobilized first surface primers, wherein the first surface primers lack a nucleotide having a scissile moiety; and
iii) continuing the rolling circle amplification reaction on the support to generate a plurality of immobilized single stranded concatemer template molecules.