Patent ID: 11926667
Assignee: JANSSEN BIOTECH, INC.
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 19:
20. A method of enriching, isolating, separating, purifying, sorting, selecting, capturing or detecting a cell, wherein
(A) the cell is a CD8-expressing cell, wherein the method comprises:
(i) providing a sample comprising the CD8-expressing cell; contacting the sample with the CD8 antibody of claim 1; and enriching, isolating, separating, purifying, sorting, selecting, capturing or detecting the CD8-expressing cell bound to the CD8 antibody,
(ii) contacting a CD8-expressing cell with the CD8 antibody of claim 1; and enriching, isolating, separating, purifying, sorting, selecting, capturing or detecting the CD8-expressing cell bound to the CD8 antibody, or
(iii) contacting a CD8-expressing cell with the CD8 antibody of claim 1; and enriching, isolating, separating, purifying, sorting, selecting, capturing or detecting the CD8-expressing cell based on binding of the CD8-expressing cell to the CD8 antibody,
wherein optionally the CD8-expressing cell is a T cell,
wherein optionally the T cell is CD8+ cytotoxic T lymphocyte (CTL);

(B) the cell is a CD4-expressing cell, wherein the method comprises:
(i) providing a sample comprising the CD4-expressing cell; contacting the sample with the CD4 antibody of claim 1; and enriching, isolating, separating, purifying, sorting, selecting, capturing or detecting the CD4-expressing cell bound to the CD4 antibody,
(ii) contacting a CD4-expressing cell with the CD4 antibody of claim 1; and enriching, isolating, separating, purifying, sorting, selecting, capturing or detecting the CD4-expressing cell bound to the CD4 antibody, or
(iii) contacting a CD4-expressing cell with the CD4 antibody of claim 1; and enriching, isolating, separating, purifying, sorting, selecting, capturing or detecting the CD4-expressing cell based on binding of the CD4-expressing cell to the CD4 antibody,

wherein optionally the CD4-expressing cell is a T cell,
wherein optionally, the T cell is a CD4+T helper cell; or
(C) the cell is a CD4-expressing cell and/or a CD8-expressing cell, wherein the method comprises:
(i) providing a sample comprising the CD4-expressing cells and/or CD8-expressing cells; contacting the sample with the multispecific antibody that binds to CD8 and CD4 of claim 1; and enriching, isolating, separating, purifying, sorting, selecting, capturing or detecting the CD4-expressing cells and/or CD8-expressing cells bound to the multispecific antibody,
(ii) contacting CD4-expressing cells and/or CD8-expressing cells with the multispecific antibody that binds to CD8 and CD4 of claim 1; and enriching, isolating, separating, purifying, sorting, selecting, capturing or detecting the CD4-expressing cells and/or CD8-expressing cells bound to the multispecific antibody, or
(iii) contacting CD4-expressing cells and/or CD8-expressing cells with the multispecific antibody that binds to CD8 and CD4 of claim 1; and

enriching, isolating, separating, purifying, sorting, selecting, capturing or detecting the CD4-expressing cells and/or CD8-expressing cells based on binding of the CD4-expressing cells and/or CD8-expressing cells to the multispecific antibody,
wherein optionally the CD4-expressing cells and/or CD8-expressing cells are T cells,
wherein optionally the CD4-expressing cells are CD4+T helper cells,
wherein optionally the CD8-expressing cells are CD8+ CTL.