Patent ID: 11873534
Assignee: ELECTROCHAEA GMBH
Field: Biotechnology (Chemistry)
Classification: CPC C  Y | IPC C

Claim 15:
16. A quality control kit for use in detecting the presence of methanogenic microorganism Methanothermobacter thermoautotrophicus DSM3590 within a given sample, the quality control kit comprising:
at least one container;
a primer pair used to PCR amplify a DNA sequence of the methanogenic microorganism Methanothermobacter thermoautotrophicus DSM3590 wherein the primer pair comprises:
a first primer serving as a forward primer and comprising SEQ ID NO: 1 and a second primer serving as a reverse primer and comprising SEQ ID NO: 4, wherein at least one of the first primer and the second primer is coupled to a detectable marker selected from the group consisting of a radioisotope, a fluorescent compound, an enzyme, and an enzyme co-factor; or
a first primer serving as a forward primer and comprising SEQ ID NO: 2 and a second primer serving as a reverse primer and comprising SEQ ID NO: 5, wherein at least one of the first primer and the second primer is coupled to a detectable marker selected from the group consisting of a radioisotope, a fluorescent compound, an enzyme, and an enzyme co-factor; or
a first primer serving as a forward primer and comprising SEQ ID NO: 3 and a second primer serving as a reverse primer and comprising SEQ ID NO: 6, wherein at least one of the first primer and the second primer is coupled to a detectable marker selected from the group consisting of a radioisotope, a fluorescent compound, an enzyme, and an enzyme co-factor;

at least one first restriction enzyme or at least one first and at least one second restriction enzyme;
optionally at least one buffer.