Patent ID: 11913055
Assignee: FUDAN UNIVERSITY
Field: Biotechnology (Chemistry)
Classification: CPC C  Y | IPC C

Claim 0:
1. A continuous-flow method for preparing (R)-3-hydroxy-5-hexenoate of formula (I) using a micro reaction system, the micro reaction system comprising a microchannel reactor,, the method comprising:
(1) co-immobilizing a carbonyl reductase and an isopropanol dehydrogenase onto an inert solid medium to prepare a carbonyl reductase/isopropanol dehydrogenase co-immobilized catalyst; and filling the microchannel reactor with the co-immobilized catalyst;
(2) pumping a substrate solution containing 3-carbonyl-5-hexenoate of formula (II) into the microchannel reactor filled with the carbonyl reductase/isopropanol dehydrogenase co-immobilized catalyst prepared in step (1) to perform an asymmetric carbonyl reduction reaction; and, (3) collecting the reaction mixture flowing out of the microchannel reactor followed by separation and purification to obtain a target product (R)-3-hydroxy-5-hexenoate;
wherein R is linear or branched C1-C8 alkyl group, linear or branched C3-C8 cycloalkyl group, monosubstituted or polysubstituted aryl group, or monosubstituted or polysubstituted aralkyl group;
wherein an amino acid sequence of the carbonyl reductase is SEQ ID NO: 1; an amino acid sequence of the isopropanol dehydrogenase is SEQ ID NO: 2; and
wherein the yield of the product (R)-3-hydroxy-5-hexenoate is increased from 80% to more than 95% compared to a method using a batch reactor.