Patent ID: 11906521
Assignee: LONZA WALKERSVILLE, INC
Field: Measurement (Instruments)
Classification: CPC G  C | IPC C  G

Claim 0:
1. A method of making limulus amebocyte lysate (LAL) substantially free of coagulogen, wherein the LAL substantially free of coagulogen comprises less than 5% (wt/wt) of coagulogen relative to total protein in the LAL as measured by SDS-PAGE with protein stain, comprising:
subjecting a composition comprising LAL, wherein the LAL comprises coagulogen, to tangential flow filtration (TFF) using a 20 kDa to 50 kDa TFF filter, wherein the TFF produces a retentate and a filtrate; and
collecting the retentate, thereby obtaining the LAL substantially free of coagulogen.