Patent ID: 11866779
Assignee: NORTHWESTERN UNIVERSITY
Field: Electrical machinery, apparatus, energy (Electrical engineering)
Classification: CPC C  H | IPC C

Claim 10:
11. A mass spectrometry method for detecting a canonical nucleobase in a nucleic acid species, comprising
a) providing a sample, wherein the sample comprises:
(i) a canonical nucleobase attached in N-glycosidic linkage to a ribose or deoxyribose moiety of a nucleic acid, nucleoside, nucleotide or oligonucleotide;
(ii) a matrix, wherein the matrix comprises at least one Bøonsted acidic proton source; and
(iii) the molar ratio of the overall nucleobases in the sample to the matrix is about 0.03 to about 0.3; and

b) subjecting the sample to at least one laser pulse, wherein the laser fluence is about 70% to about 95% of the matrix storm level.