Patent ID: 11859211
Assignee: PRESIDENT AND FELLOWS OF HARVARD COLLEGE
Field: Pharmaceuticals (Chemistry)
Classification: CPC C  A | IPC A  C

Claim 23:
24. A method for treatment of a muscle disorder in a subject in need thereof, the method comprising administering to the subject a pharmaceutical composition comprising therapeutically effective amount of an isolated population of non-naturally occurring satellite cells produced according to a method comprising:
contacting a population of cells comprising myoblasts in spin culture with at least one medium for a time sufficient to induce the dedifferentiation of at least one myoblast in the spin culture into a non-naturally occurring satellite cell, wherein:
the non-naturally occurring satellite cells produced have a gene expression profile that is different from the gene expression profile of an endogenous satellite cell;
the non-naturally occurring satellite cells produced express two or more genes selected from the group consisting of: Nr2f1, Dmpl, Wisp2/Ccn5, Ecm1, Map, Igfbp5, Itgb5, Sdc2 and Bgn, wherein the expression of the two or more genes is upregulated by greater than 2-fold relative to expression by myoblasts and the two or more genes are not expressed in an endogenous satellite cell.