Patent ID: 11933707
Assignee: CHEMOMETEC A/S
Field: Measurement (Instruments)
Classification: CPC G | IPC G

Claim 16:
17. A method for characterising one or more objects in a sample performed by an image cytometer or a microscope, the method comprising the steps of:
collecting transmitted, refracted, scattered, diffracted, or emitted light from the sample, wherein the light comprises fluorescent or luminescent light, using one or more lens(es), thus forming collected light,
forming by said collected light on a sensor surface
at least a first image and a second image of the object(s), wherein for at least the first and the second image the collected light is modulated asymmetrically differently after the sample, and

processing
the at least first and second images by comparing the first image with the second image for
determining a position along an axis of at least one of the objects based on how much the at least one object has moved between the at least first image and the at least second image, wherein the axis is defined by a direction of the light from the sample along the one or more lens(es);

wherein the one or more objects is a cell or a particle, element or region of a cell.