Patent ID: 11913137
Assignee: BRISTOL-MYERS SQUIBB COMPANY
Field: Measurement (Instruments)
Classification: CPC C  G | IPC C  G

Claim 3:
4. A method for selecting a target-binding protein with favorable biophysical properties comprising:
(i) selecting a first population of target-binding protein variants consisting of at most 105 target-binding protein variants from a library comprising at least about 1012 protein variants using mRNA display, comprising conducting a number of rounds of selection sufficient to obtain the at most 105 target-binding protein variants, wherein the library of protein variants is a library of 10th domain of fibronectin type III (10Fn3) variants;
(ii) determining whether a significant number of the at most 105 target-binding protein variants have favorable biophysical properties, wherein the favorable biophysical properties are one or more of solubility, thermostability, high expression levels, low levels of aggregation, proper folding, and binding specificity; and
(iii) if a significant number of the at most 105 target-binding protein variants do not have favorable biophysical properties, then selecting a population of at most 103 target-binding protein variants from the at most 105 target-binding protein variants obtained during the selection in step (i) using yeast surface display.