Patent ID: 11896005
Assignee: OSSIUM HEALTH, INC.
Field: Basic materials chemistry (Chemistry)
Classification: CPC A  C | IPC A  C

Claim 21:
22. A method comprising:
(a) placing a cadaver bone in a closed container comprising a cryoprotectant solution;
(b) reducing the pressure in the closed container and, optionally, holding the closed container at reduced pressure, to remove at least a portion of the water present in the cadaver bone;
(c) raising the pressure in the closed container and holding the closed container at a raised pressure to allow infiltration of the cryoprotectant solution into the cadaver bone;
(d) removing the cadaver bone from the closed container;
(e) chilling the cadaver bone to a temperature of at least below 0° C., thereby cryopreserving the cadaver bone;
(f) storing the cryopreserved cadaver bone at a temperature of at least below 0° C.;
(g) dividing the cryopreserved cadaver bone to obtain fragments of the cryopreserved bone; and
(h) transferring the fragments of the cryopreserved bone into a grinding medium having a temperature of from about 35° C. to about 45° C. for a time sufficient to warm the cadaver bone fragments to a surface temperature of about 20° C.