Patent ID: 11905310
Assignee: UCB BIOPHARMA SRL
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 10:
11. A process for the purification of a protein of interest from a mixture comprising the steps of:
a) in an operational chromatography cycle loading a first volume of a mixture containing the protein of interest from a first container to a chromatography matrix operated such that the dynamic binding capacity of the chromatography matrix for the protein of interest is exceeded;
b) collecting flow-through containing unbound protein of interest in a second container, and
c) in a further operational chromatography cycle re-loading the flow-through collected in the second container to the same chromatography matrix followed by loading a second volume of the protein of interest from the first container to the same chromatography matrix, operated such that the dynamic binding capacity of the chromatography matrix for the protein of interest is exceeded.