Patent ID: 11963989
Assignee: GUANGDONG XINGHAI BIOTECHNOLOGY CO., LTD.
Field: Pharmaceuticals (Chemistry)
Classification: CPC A  C  Y | IPC A  C

Claim 0:
1. A method of preparing a blood lipid-lowering drug, comprising:
providing a medical cell Lactobacillus rhamnosus CMU-pb-7, the medical cell Lactobacillus rhamnosus CMU-pb-7 being deposited at the China Center for Type Culture Collection (CCTCC) under accession number M 2022220,
wherein providing the medical cell Lactobacillus rhamnosus CMU-pb-7 comprises:
(1) collecting fresh feces of healthy people in a morning of a day through sampling, and separating fecal bacteria immediately;
(2) adding an MRS broth medium containing glycerol as a cryoprotectant to the separated fecal bacteria to obtain a crude fecal bacteria solution;
(3) adding the crude fecal bacteria solution into normal saline, and mixing thoroughly to a concentration of 10−3 to 10−7;
(4) applying the crude fecal bacterial solution to an MRS agar medium, a TPY agar medium and an M17 agar medium, and culturing at 37° C. under anaerobic conditions for 48 to 72 hours;
(5) selecting pure cultures of single colonies on corresponding agar media to obtain the medical cell Lactobacillus rhamnosus CMU-pb-7.