Patent ID: 11932858
Assignee: nan
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 0:
1. A homologous recombination composition for identifying a cell comprising an accurate homologous recombination event, the recombination composition comprising:
a. a homologous recombination system comprising:
i. an expression construct comprising a promoter operably linked to a nucleic acid sequence encoding a programmable nucleic acid modification system, wherein the modification system targets a nucleic acid locus in a gene of interest; and
ii. a donor polynucleotide comprising a nucleic acid sequence encoding a reporter flanked by regions homologous to the nucleic acid locus in the gene of interest; and

b. an expression construct comprising a promoter operably linked to a nucleic acid sequence encoding a transcription activation system specific for inducing expression of the gene of interest;
wherein expression of the transcription activation system in a cell induces expression of the gene of interest, wherein expression of the transcription activation system in the cell does not induce expression of the reporter indicates an inaccurate recombination event, wherein expression of the transcription activation system in the cell induces expression of the reporter indicates an accurate recombination event, and wherein expression of the reporter identifies a cell comprising an accurate homologous recombination event.