Patent ID: 11872275
Assignee: ENGINEERED ANTIGENS INC.
Field: Pharmaceuticals (Chemistry)
Classification: CPC A  C  Y | IPC A  C

Claim 0:
1. A method of causing transport of a target protein from the cytosol to the extracellular surface of a host cell comprising the target protein, the method comprising:
(a) providing a chimeric polynucleotide comprising as operably linked components:
(i) a polynucleotide capable of controlling expression in the host cell; and
(ii) a polynucleotide encoding a Surface Lipoprotein Assembly Modulator (SLAM) polypeptide, wherein the polynucleotide comprises a polynucleotide sequence selected from the group consisting of SEQ.ID NO: 5; SEQ.ID NO: 199; SEQ.ID NO: 273; SEQ.ID NO: 385; SEQ.ID NO: 387; SEQ.ID NO: 1087; SEQ.ID NO: 1111; and SEQ.ID NO: 1181, ora polynucleotide sequence that encodes a polypeptide that is at least 90% identical to a polypeptide encoded by any of the foregoing polynucleotides; and

(b) introducing the chimeric polynucleotide in the host cell and growing the host cell to express and produce the SLAM polypeptide, thereby causing transport of the target protein from the cytosol to the extracellular surface of the host cell.