Patent ID: 11952432
Assignee: DANA-FARBER CANCER INSTITUTE, INC.
Field: Pharmaceuticals (Chemistry)
Classification: CPC C  A | IPC A  C

Claim 6:
7. A method of delivering an agent into a cell, the method comprising:
contacting the cell with a compound comprising (1) the agent and (2) a cell-permeable stapled peptide, wherein the agent is linked to the cell-permeable stapled peptide, wherein the cell-permeable stapled peptide comprises an internally cross-linked polypeptide comprising any one of the amino acid sequences: (i) X1R2R3R2X5R2B (SEQ ID NO: 3), (ii) X1R2R3R2X5B (SEQ ID NO: 4), (iii) X1R2R2R2X5R2B (SEQ ID NO: 7), (iv) X1R2R3R2X4R2BGBR2X5R2R3R2X6 (SEQ ID NO: 8), or (v) X1R2R3R2X4R2BGX5R2R3R2X6R2B (SEQ ID NO: 9),
wherein B is Norleucine;
wherein R2 is L-Arg, R3 is D-Arg;
wherein X1, X4, X5, and X6 are each (S)-2-(4′-pentenyl) alanine; and
wherein X1 and X4 and X5 and X6 are cross-linked.