Patent ID: 11952624
Assignee: GENESENSE TECHNOLOGY INC., SHANGHAI (CN)
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 15:
16. A method of incorporating a nucleotide derivative into a nucleic acid sequence, comprising:
combining within a reaction vessel a thermophilic nucleic acid polymerase, a primer hybridized to a portion of the nucleic acid sequence, and a nucleotide derivative, and allowing said thermophilic nucleic acid polymerase to incorporate said nucleotide derivative into said primer thereby incorporating a nucleotide derivative into a nucleic acid sequence, wherein said nucleotide derivative is one of four compounds having the general formula:

NT-L1-L2-Lb, wherein NT is a nucleotide selected from A, T(U), C, and G, and the hydroxyl (—OH) at the 3′-position of ribose or deoxyribose of the nucleotide is protected by a reversible protecting group;, wherein L1 is a first linker, L2 is a second linker, and Lb is a molecular label binding to or reactive to a receptor in a detectable group comprising an luminescence-activating molecule and the receptor, the luminescence-activating molecule capable of causing emission of fluorescence when bound to a suitable substrate without being excited by external photoexcitation; and, wherein L2 is different for different nucleotides, and at least three of the four second linkers are each cleavable under a specific reaction condition under which L2 in other three of the four compounds as well as the first linker of any of the four compounds are not cleaved.