Patent ID: 11926854
Assignee: MERCK PATENT GMBH
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 0:
1. A split intein-based affinity chromatography system, comprising:
a) a fusion protein comprising a C-intein polypeptide joined to a target molecule by a peptide bond; and
b) an affinity chromatography matrix comprising an N-intein polypeptide capable of forming an active intein complex by associating with a fusion protein comprising the C-intein polypeptide and comprising at least one cysteine substituted with an amino acid selected from the group consisting of: threonine, lysine, asparagine, serine, methionine, and tyrosine, wherein the N-intein polypeptide is attached by covalent attachment to a solid support.