Patent ID: 11898148
Assignee: CARRYGENES BIOENGINEERING, LLC
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 19:
20. The method of claim 12, further comprising the steps of:
identifying a third group of cells in which the first, the second and the third genes of interest have been loaded onto the autonomously replicating synthetic chromosome and the recyclable marker gene cassette is no longer present;
in the identified third group of cells, using the first recombination system to integrate a fourth delivery vector comprising the recyclable marker gene cassette and a fourth gene of interest into a fourth of the multiple recombination target sites on the autonomously replicating synthetic chromosome; and
with the second recombination system using signal sites delta, excising the recyclable marker gene cassette from the autonomously replicating synthetic chromosome, wherein the fourth gene of interest remains on the autonomously replicating synthetic chromosome.