Patent ID: 11965173
Assignee: AUTOLUS LIMITED
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 0:
1. A plasmid system for transfection into a cell to create a producer cell, the system comprising:
a) a helper plasmid comprising a first nucleotide sequence encoding Murine leukemia virus (MLV)-derived Gag and Pol poly-proteins,
b) an envelope plasmid comprising a second nucleotide sequence encoding an Env protein,
c) a genome plasmid comprising a third nucleotide sequence comprising a retroviral genome,
wherein the first nucleotide sequence comprises the sequence selected from SEQ ID NO: 1, SEQ ID NO: 2 or SEQ ID NO: 3, and
wherein the second nucleotide sequence is codon-optimized for expression in the producer cell.