Patent ID: 11958880
Assignee: LUDWIG-MAXIMILIANS-UNIVERSITÄT MÜNCHEN
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 2:
3. A method for the production of a polypeptide comprising
(a′) introducing or adding at the C-terminus of a polypeptide a recognition sequence for tubulin tyrosine ligase, wherein the recognition sequence comprises the amino acid sequence X4X3X2X1 (SEQ ID No. 11), wherein X4 is E, D, A, K, or P X3 is G, S, A, V or F, X2 is E, D or C and X1 is E; and
(b′) contacting the polypeptide obtained in step (a′) in the presence of tubulin tyrosine ligase and a tyrosine derivative conjugated to a moiety under conditions suitable for the tubulin tyrosine ligase to tyrosinate said polypeptide with said tyrosine derivative conjugated to said moiety,
wherein said polypeptide is an antibody or fragment thereof,
wherein said tyrosine derivative is tyrosine derivative 1:,