Patent ID: 11971336
Assignee: SHIMADZU CORPORATION
Field: Measurement (Instruments)
Classification: CPC G  C  H | IPC C  G  H

Claim 0:
1. A method for preparing a sample comprising a glycan, the method comprising:
performing a lactonization reaction in which at least a part of sialic acids included in the glycan is lactonized; and
performing an amidation reaction in which lactones of lactonized sialic acids are amidated by aminolysis based on an interaction between an amino group and a lactone through addition of an amidation reaction solution to the sample, wherein the amidation reaction solution comprises ammonia, an amine, or a salt thereof is reacted with the lactonized sialic acids, wherein the concentration of ammonia, an amine, or a salt thereof in the amidation reaction solution is 0.5 M or more, wherein:
the amine is a primary amine;
pH of the amidation reaction solution is 7.7 or higher; and
in the lactonization reaction, at least one selected from the group consisting of α2,3-sialic acid, α2,8-sialic acid, and α2,9-sialic acid among the sialic acids is lactonized, wherein an operation that reacts the sample with a dehydration condensation agent is not performed after the addition of the amidation reaction solution to the sample, wherein: the amidation reaction solution does not include a dehydration condensation agent that is reacted with the lactones, and wherein only contacting the sample with the amidation reaction solution is performed for the amidation reaction.