Patent ID: 11932886
Assignee: ENZYMASTER (NINGBO) BIO-ENGINEERING CO., LTD.
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 4:
5. A process of preparing a chiral amine product of formula (I):, wherein the amine products of formula (I) have the indicated stereochemical configuration shown at the chiral center marked with an* and the amine products of formula (I) are in an enantiomeric excess over the other isomer, further wherein
R1 is an optionally substituted or unsubstituted aryl or heteroaryl, or optionally substituted or unsubstituted C1-C8 hydrocarbyl;
R2 is an optionally substituted or unsubstituted C1-C6 hydrocarbyl, a halogen selected from among —F, —Cl, —Br, and —I, an —NO2, —NO, —SO2R′ or —SOR′, —SR′, —NR′R′, —OR′, —CO2R′ or —COR′, —C(O)NR′, —SO2NH2 or —SONH2, —CN, CF3; wherein each R′ is independently selected from —H or (C1-C4) hydrocarbyl,
said the process comprising the steps of contacting, under suitable reaction conditions comprising a reaction solvent, an amine donor and the ketone substrate of formula (II), with the transaminase polypeptide of claim 1 and converting the ketone substrate to the chiral amine product.