Patent ID: 11965877
Assignee: BIO-RAD LABORATORIES, INC.
Field: Measurement (Instruments)
Classification: CPC G  C | IPC C  G

Claim 5:
6. A method for barcoding a target sequence from a single cell, the method comprising:
introducing a plurality of nucleic acid constructs not attached to microbeads into a plurality of aqueous droplets in oil, each droplet containing a plurality of RNA molecules from a single cell and a plurality of the nucleic acid constructs not attached to microbeads, wherein each of the nucleic acid constructs within the same droplet comprises a universal priming site, a barcode sequence, and a random hexamer, wherein the barcode sequence is a unique barcode that allows sequence reads to be assigned to one molecule from the cell;
hybridizing a nucleic acid construct to a target sequence in at least one droplet to form a hybridized nucleic acid construct that is not attached to a microbead;
extending the hybridized nucleic acid construct to produce a barcoded extension product not attached to a microbead; and
amplifying, using the universal priming site, the barcoded extension product to yield an amplified product.