Patent ID: 11860354
Assignee: SCANOGEN INC.
Field: Optics (Instruments)
Classification: CPC G | IPC G

Claim 9:
10. A method of determining a position or a movement of a particle in a sample, comprising the steps of:
generating a blurred image of the particle, wherein a dimension of the blurred image of the particle is greater than the dimension of the particle multiplied by the magnification of the lens, and wherein a field of view in an image plane of the lens is larger than twice a size of the particle;
sensing the image of the particle on at least two pixels of an image sensor having a pixel size of less than about the dimension of the image of the particle wherein an intensity of the image of the particle is distributed on at least two pixels to form an intensity distribution; and
processing the image of the sample, including: determining a center of the intensity distribution associated with a first position of the particle, and determining a displacement from the first position of the particle to a second position of the particle in a second configuration of the sample; the displacement being indicative of a binding of the particle to a target-probe complex.