Patent ID: 11913070
Assignee: ARIZONA BOARD OF REGENTS ON BEHALF OF ARIZONA STATE UNIVERSITY
Field: Measurement (Instruments)
Classification: CPC C  G | IPC C  G

Claim 18:
19. A method of calibrating a bioelectronic device, the method comprising:
(a) introducing a template polynucleotide to the bioelectronic device, wherein the bioelectronic device comprises a polymerase functionally coupled to at least a first electrode and a second electrode;
(b) introducing a solution comprising dNTP monomers to the device comprising the template polynucleotide, each dNTP present in the solution at a saturating concentration;
(c) obtaining a bioelectronic signature of polymerase activity based on current fluctuations as each complementary dNTP monomer is incorporated into the template polynucleotide, wherein the bioelectronic signature comprises an open period corresponding to the polymerase being in an open state; and
(d) measuring or determining the intrinsic distribution of the open periods for each dNTP, wherein the bioelectronic device is calibrated based on the distribution of open periods.