Patent ID: 11879128
Assignee: CONSEJO SUPERIOR DE INVESTIGACIONES CIENTÍFICAS (CSIC)—DELEGACIÓN ANDALUCÍA
Field: Biotechnology (Chemistry)
Classification: CPC C  A | IPC A  C

Claim 17:
18. A method for obtaining the genetically altered plant as defined in claim 9, the method comprising:
a. selecting a part of the plant;
b. transfecting at least one cell of the part of the plant of paragraph (a) with the nucleic acid construct as defined in from SEQ ID Nos 25 to 28 and 30 to 48 and 807 to 809 and 811 to 818 or a variant thereof or at least one sgRNA molecule as defined in any of SEQ ID NOs 75 to 78 and 80 to 99 or a variant thereof wherein the variant has at least 90% sequence identity over the full length non-variant sequence;
c. regenerating at least one plant derived from the transfected cell or cells;
d. selecting one or more plants obtained according to paragraph (c) that show silencing or reduced expression and/or content of at least one of alpha-, gamma- and/or omega gliadins, reduced total gliadin content, reduced gluten content, a reduced gliadin to glutenin ratio and/or increased expression and/or content of glutenins.