Patent ID: 11885814
Assignee: FRED HUTCHINSON CANCER CENTER
Field: Measurement (Instruments)
Classification: CPC G  C | IPC C  G

Claim 31:
32. A method for detecting the binding of a chromatin-associated factor of interest to a sequence of chromatin DNA in a cell or nucleus, comprising:
contacting an unfixed permeabilized cell or nucleus with a first specific binding agent that specifically recognizes the chromatin-associated factor of interest, wherein the first specific binding agent is coupled to a nuclease in an inactive state;
activating the nuclease, thereby excising the DNA bound to the chromatin-associated factor of interest;
releasing the DNA to a supernatant;
isolating the excised DNA in the supernatant; and
determining the sequence of the excised DNA, thereby mapping binding of a chromatin-associated factor of interest to a sequence of DNA in the cell or nucleus.