Patent ID: 11939562
Assignee: PLURI BIOTECH LTD.
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 0:
1. A three-dimensional (3D) bioreactor for large scale expansion of immune cells, the 3D bioreactor comprising:
at least one packed bed chamber;
at least one porous scaffold surrounded by said at least one packed bed chamber, wherein said at least one porous scaffold is coated with one or more extra cellular matrix protein (ECM);
at least one container surrounding said at least one packed bed chamber; and
a fluid media having at least one population of immune cells suspended in said fluid media contained in said at least one container;
wherein said fluid media having at least one population of immune cells suspended in said fluid media flows through said packed bed chamber and said at least one porous scaffold coated with one or more ECM
wherein, said at least one porous scaffold coated with one or more ECM creates a stationary niche having low shear forces that imitate the natural growth environment of said population of immune cells; and
wherein said at least one porous scaffold coated with one or more ECM has a porosity which allows expansion of the at least one population of immune cells that flow through said at least one porous scaffold coated with one or more ECM in large scale without immobilizing said at least one population of immune cells.