Patent ID: 11926840
Assignee: TOKIWA-BIO INC.
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 0:
1. A stealth RNA gene expression system comprising:
a negative-sense single-stranded RNA (A),
a single-stranded RNA binding protein (B), and
an RNA-dependent RNA polymerase (C),
wherein the stealth RNA gene expression system is a complex that does not activate an innate immune system,
wherein the negative-sense single-stranded RNA (A) comprises RNA sequences (1) to (8):
(1) RNA sequences encoding any given protein or functional RNA,
(2) RNA sequences constituting noncoding region(s), the RNA sequences (2) having a length of 5 nucleotides to 49 nucleotides and being a partial mRNA(s) of an animal gene expressed in animal cells,
(3) transcription start signal sequences recognized by the RNA-dependent RNA polymerase,
(4) transcription termination signal sequences recognized by the RNA-dependent RNA polymerase,
(5) RNA sequences comprising replication origins recognized by the RNA-dependent RNA polymerase,
(6) RNA sequences encoding the RNA-dependent RNA polymerase with codons optimized for a biological species of cells for transfection,
(7) an RNA sequence encoding a protein that regulates activity of the RNA-dependent RNA polymerase with codons optimized for the biological species of the cells for transfection, and
(8) an RNA sequence encoding the single-stranded RNA binding protein with codons optimized for the biological species of cells for transfection,
wherein the RNA-dependent RNA polymerase encoded by the RNA sequences of the (6) consists of L protein and P protein of an RNA virus belonging to a paramyxovirus family,
wherein the protein that regulates activity of the RNA-dependent RNA polymerase encoded by the RNA sequence of the (7) is C protein of the RNA virus belonging to the paramyxovirus family,
wherein the single-stranded RNA binding protein encoded by the RNA sequence of the (8) is NP protein of the RNA virus belonging to the paramyxovirus family, and
wherein all of the RNA sequences of the (3) to (5) are RNA sequences comprising a transcription start signal, a transcription termination signal, or a replication origin sequence isolated from a genome of the same virus as the RNA virus.