Patent ID: 11912982
Assignee: MODERNATX, INC.
Field: Measurement (Instruments)
Classification: CPC C  G | IPC C  G

Claim 0:
1. A method for separating a nucleic acid having a hydrophobic portion from a mixture comprising one or more additional nucleic acids or impurities, the method comprising:
(i) contacting a hydrophobic stationary phase of a reverse phase chromatography column with the mixture; and
(ii) eluting the nucleic acid having a hydrophobic portion with a mobile phase, wherein the mobile phase: (a) comprises an ion pairing agent selected from (i) Tris, (ii) inorganic cations, and (iii) biological buffers, and (b) lacks triethylammonium salts, tetrabutylammonium salts, hexylammonium salts, and dibutylammonium salts; such that the nucleic acid having a hydrophobic portion traverses the column with a retention time that is different than the one or more additional nucleic acids or impurities of the mixture.