Patent ID: 11938186
Assignee: INDIAN INSTITUTE OF TECHNOLOGY HYDERABAD (IITH)
Field: Pharmaceuticals (Chemistry)
Classification: CPC A | IPC A

Claim 10:
11. A method for preparing the thermosensitive hydrogel as claimed in claim 1 containing gold-coated liposomal nanoparticles and doxorubicin, comprising:
a. adding polyvinyl alcohol to a solution of poloxamer 407 under constant stirring at a temperature in the range from 2-6° C., wherein the concentration of polyvinyl alcohol is in a range of 2-5% v/v and the concentration of poloxamer 407 is in a range of 22-25% wt/v;
b. adding 2-8% solution of ice cold bovine serum albumin to the reaction mixture obtained in step (a) under vigorous stirring for about 6 hrs to obtain a thermosensitive hydrogel;
c. adding gold coated liposomal nanoparticles to the hydrogel obtained in step (b) at a temperature about 4° C. under constant stirring for 10 minutes, wherein the concentration of gold-coated nanoparticles ranges from 1-3 mg/mL to obtain hydrogel containing gold-coated liposomal nanoparticles; and
d. adding doxorubicin to the gold-coated liposomal nanoparticles liposomal nanoparticles, wherein doxorubicin is present at concentration in the range from 50-500 μg/mL to obtain hydrogel containing gold-coated liposomal nanoparticles and doxorubicin.