Patent ID: 11931417
Assignee: NANSHA BIOLOGICS (HONG KONG) LIMITED
Field: Pharmaceuticals (Chemistry)
Classification: CPC A  C | IPC A  C

Claim 0:
1. A method of preparing an interleukin 11 (IL-11) conjugate, comprising:
contacting an IL-11 polypeptide chain with a PEG moiety comprising an amine-selective reactive group at an equimolar to 2-fold molar excess of the PEG moiety relative to the IL-11 peptide a pH of from 4.5 to 5.0 and ambient temperature for at least 24 hours, wherein the PEG moiety has an average molecular weight of 10 kD to 50 kD and has at least two branches; and
collecting a resulting IL-11 conjugate preparation, wherein the resulting IL-11 conjugate preparation comprises a monosubstituted IL-11 conjugate at a purity of greater than 93%,
wherein the IL-11 polypeptide chain is a human IL-11 or truncated human IL-11 polypeptide chain.