Patent ID: 11965154
Assignee: INSCRIPTA, INC.
Field: Measurement (Instruments)
Classification: CPC C  B  G | IPC B  C  G

Claim 8:
9. A method for performing enrichment of cells edited by a nucleic acid-guided nuclease comprising:
(a) providing transformed cells in growth medium, wherein the cells comprise a first round of nucleic acid-guided editing components, wherein the first round of nucleic acid-guided editing components comprises a first gRNA under the control of a first inducible promoter;
(b) allowing the transformed cells to grow until the cells are in stationary phase;
(c) inducing transcription of the first gRNA;
(d) allowing the cells to edit, wherein an editing efficiency is improved compared to inducing transcription of the first gRNA when the transformed cells are in log phase; and
(e) transforming the cells with a second round of nucleic acid-guided editing components, wherein the second round of nucleic acid-guided editing components comprises a second gRNA under the control of a second inducible promoter.