Patent ID: 11965158
Assignee: ILLUMINA, INC.
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 18:
19. A system for obtaining captured contiguity information from a double-stranded target nucleic acid comprising:
a polynucleotide comprising a single target nucleic acid sequence;
a plurality of transposomes, wherein each transposome comprises a transposase and a transposon nucleic acid, wherein the transposase comprises an activity to nick only one strand of the double-stranded target nucleic acid; and
a plurality of polynucleotide fragments prepared with the plurality of transposomes, wherein the plurality of polynucleotide fragments is immobilized on a surface and comprises overlapping sequences in a linear representation of the single target nucleic acid sequence, wherein at least one end of the plurality of polynucleotide fragments comprises the transposon nucleic acid, and wherein immobilized polynucleotide fragments are distributed on the surface such that immobilized polynucleotide fragments immobilized at sites closer to one another on the surface comprise sequences located closer to one another in the linear representation of the target nucleic acid sequence compared to immobilized polynucleotide fragments immobilized at sites less close to one another on the surface and comprising sequences located less close to one another in the linear representation of the target nucleic acid sequence.