Patent ID: 11926817
Assignee: NUTCRACKER THERAPEUTICS, INC.
Field: Measurement (Instruments)
Classification: CPC A  C  B  G | IPC B  C  G

Claim 1:
2. A microfluidic apparatus for forming a therapeutic polynucleotide, the apparatus comprising:
a seating mount for removably holding a microfluidic path device;
a plurality of pressure lines;
a plurality of fluid vials, wherein each fluid vial either comprises a fluidic line or is configured to couple with the fluidic line,
wherein each fluidic line and at least a subset of the pressure lines are configured to be biased against the microfluidic path device held in the seating mount to form a closed fluid path;
an optical sensor drive configured to move one or more of a plurality of optical sensors around the seating mount or around the plurality of vials; and
a controller comprising one or more processors, the one or more processors programmed to control the application of pressure through the pressure lines to drive fluidic movement in the microfluidic path device when the microfluidic path device is held in the seating mount, wherein the controller is configured to determine the contents of the fluid vials, transfer sub-microliter amounts of material from the fluid vials to one or more reactors in the microfluidic path device held in the seating mount, direct the synthesis of a synthetic template, direct an in vitro transcription (IVT) reaction using the template to form a therapeutic polynucleotide, and direct purification of the therapeutic polynucleotide in one or more microfluidic path devices held in the seating mount.