Patent ID: 11905557
Assignee: LIFE TECHNOLOGIES CORPORATION
Field: Biotechnology (Chemistry)
Classification: CPC C  B | IPC B  C

Claim 0:
1. A method comprising:
performing a single-stranded DNA sequencing assay to yield a product mixture;
introducing the product mixture to an inlet of a flow channel in a microfluidic chip;
flowing the product mixture through a first section along a length of the flow channel and into contact with a first microstructure in the first section, the first microstructure coated with a first reagent comprising an anion-exchange reagent configured to capture negatively-charged single-stranded DNA fragments from the product mixture, and the first microstructure coated with a second reagent comprising a size-exclusion reagent configured to prevent single-stranded DNA fragments larger than a preselected size from being captured from the product mixture;
capturing negatively-charged single-stranded DNA fragments from the product mixture via the first microstructure to yield a first filtrate;
after the capturing of the negatively-charged single-stranded DNA fragments, flowing the first filtrate through a second section along the length of the flow channel and into contact with a second microstructure in the second section, the second microstructure coated with a third reagent comprising a cation-exchange reagent configured to capture positively-charged single-stranded DNA fragments; and
capturing positively-charged single-stranded DNA fragments from the first filtrate via the second microstructure to yield a second filtrate.