Patent ID: 11906432
Assignee: ESSENLIX CORPORATION
Field: Measurement (Instruments)
Classification: CPC G | IPC G

Claim 65:
66. A device for a rapid homogenous detection of an analyte inside a membrane of a cell in a sample, comprising:
(a) a first plate and a second plate, each has a sample contact area on its surface, wherein the sample contact surfaces contact a sample comprising a cell that contains or is suspected of containing an analyte inside of the cell membrane;
(b) a detection probe that specifically binds the analyte and is capable of emitting a light at a wavelength, wherein the detection probe diffuses in the sample;
(c) a permeabilization agent that makes a membrane of the cell permeable to the detection probe;
wherein the first and second plates are configured to sandwich the sample, the detection probe, and the permeabilization agent between the two sample contact areas of the two plates to form a thin layer of a thickness of 200 microns (um) or less;
wherein the thin layer sample thickness is configured so that for a given concentration of the cell in the sample, each individual cell does not substantially overlap other cells in an imaging, wherein the imaging is performed without a washing step;
wherein the thickness of the thin layer and the concentration of the detection probe in the sample, are configured to make, in the imaging, in the thin layer, the location having the detection probe that bound to the analyte inside the cell membrane is distinguishable from the locations that do not have the cell; and
wherein the washing step is a step for removing unbound detection probe, permeabilization reagent, or both from the sample contact areas.