Patent ID: 11878082
Assignee: CHENGDU BANGJIALEJUN BIOTECHNOLOGY CO., LTD
Field: Pharmaceuticals (Chemistry)
Classification: CPC A  Y | IPC A

Claim 5:
6. A method of encapsulating probiotics, comprising the following steps:
Step S1, mixing a metal salt aqueous solution with a probiotics solution fully, adding a biological macromolecular aqueous solution, adjusting a pH value, and carrying out washing to obtain a probiotics solution containing the probiotics coated with a first layer of a nano-film wherein a concentration of the probiotics solution obtained is 1*106-1*109 CFU/mL, a concentration of the metal salt aqueous solution is 1-10 mg/mL, a concentration of the biological macromolecular aqueous solution is 10-100 mg/mL, a volume ratio of the metal salt aqueous solution to the probiotics solution is 1:50, and a volume ratio of the biological macromolecular aqueous solution to the probiotics solution obtained in the step S1 is 1:50;
Step S2, adjusting the concentration of the probiotics solution containing the probiotics coated by the first layer of the nano-film obtained in the step S1, and repeatedly operating the step S1 for 0-N times; and
Step S3, adjusting the concentration of the probiotics solution containing the probiotics coated by the first layer of the nano-film obtained in the step S2, adding a bio-enzyme aqueous solution, to form the probiotics coated with the second layer of the nano-film, and carrying out freeze drying to obtain the encapsulated probiotics;
wherein the concentration of the probiotics solution containing the probiotics coated with the first layer of the nano-film after adjustment in the steps S2 and S3 is 1*106-1*109 CFU/mL, a concentration of the bio-enzyme aqueous solution in the step S3 is 1-20 mg/mL, and a volume ratio of the bio-enzyme aqueous solution to the probiotics solution containing the probiotics coated with the first layer of the nano-film is 1:50,
wherein the first layer is formed from covalent cross-linking or metal chelating action in situ between metal ions of the metal salt aqueous solution and biological macromolecules of the macromolecular aqueous solution, wherein the metal ions are selected from the group consisting of cations of Al, Fe, Zn, Mn, Ni, Co and V and combinations thereof, wherein the biological macromolecules are selected from the group consisting of polyphenol, dopamine, dopamine derivatives, polysaccharide biomass, and combinations thereof, and
wherein the bio-enzymes of the bio-enzyme aqueous solution are selected from the group consisting of β-galactosidase, amylopsin, α-glucosaccharase, β-glucosaccharase, pancreatic lipase, trypsin, cellulose, and combinations thereof.