Patent ID: 11933729
Assignee: ABBERIOR INSTRUMENTS GMBH
Field: Measurement (Instruments)
Classification: CPC G | IPC G

Claim 0:
1. A method for adapting an estimator for use in a microscope for estimating a position of an emitter in a sample based on a method, in which the sample is illuminated with light at one or more sets of probe positions (Pi) and fluorescence photons are acquired for the sets of probe positions (Pi), comprising:
illuminating the sample with light at one or more sets of probe positions (Pi);
acquiring fluorescence photons for the sets of probe positions;
adding photon counts of the acquired photons to vectors of photon counts or sums of photon counts for the sets of probe positions;
determining a value representative of background noise from the vectors of photon counts or the sums of photon counts; and
adapting the estimator in real-time using the value representative of background noise.