Patent ID: 11865539
Assignee: MURSLA LIMITED
Field: Measurement (Instruments)
Classification: CPC B  G | IPC B  G

Claim 0:
1. A method of detecting one or more target biological entities in a biofluid using a sensor, wherein the biofluid comprises a plurality of the target biological entities and nanoparticles, the sensor comprising a substrate bearing a pair of electrodes having an affinity with the nanoparticles, and wherein a region between the electrodes defines a sensing region, the method comprising:
treating the biofluid with a suspension comprising a plurality of nanoparticles to obtain a treated mixture comprising bound nanoparticle-entity assemblies;
introducing the treated mixture to the sensor, wherein introducing the treated mixture to the sensor comprises applying an electric field to the treated mixture to concentrate the bound nanoparticle-entity assemblies in the sensing region;
conditioning the sensor in the presence of the treated mixture by applying an activation voltage between the electrodes to increase a degree of connection between a surface of the pair of electrodes and at least one bound nanoparticle-entity assembly in contact with the surface of the pair of electrodes, wherein the conditioning comprises applying an activation voltage sufficient to fuse the bound nanoparticle-entity assemblies into a link or chain of one or more of the bound nanoparticle-entity assemblies linking the pair of electrodes; and
detecting a presence of the one or more target biological entities by using the pair of electrodes to detect a current through the at least one bound nanoparticle-entity assembly.