Patent ID: 11932871
Assignee: YEDA RESEARCH AND DEVELOPMENT CO. LTD.
Field: Pharmaceuticals (Chemistry)
Classification: CPC C  A | IPC A  C

Claim 7:
8. A method of producing engineered T cells, the method comprising transducing T cells with a nucleic acid encoding an expression product of interest and further comprising culturing said T cells in a culture vessel in the presence of an exogenous chemokine, an exogenous adhesion molecule and a T cell stimulator, thereby producing the engineered T cells, wherein said chemokine is CCL21, and said adhesion molecule is ICAM1, and wherein each of said chemokine and adhesion molecule are immobilized to a solid support, wherein said T cell stimulator is an antigen-specific stimulator comprising a dendritic cell loaded with an antigen, wherein said immobilized CCL21 and ICAM 1 are attached to said culture vessel, and wherein said expression product of interest is a T cell receptor (TCR) or a chimeric antigen receptor (CAR).