Patent ID: 11965153
Assignee: REGENERON PHARMACEUTICALS, INC.
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 0:
1. A method of controlling a bioreactor system, comprising:
providing a cell culture in a perfusion bioreactor, wherein conditions in the perfusion bioreactor enable the cell culture to produce a protein of interest;
measuring one or more process parameters of the cell culture by a RAMAN probe;
removing cell-free spent media from the cell culture using a first output conduit at a first specified rate;
removing cells from the cell culture using a second output conduit at a second specified rate;
introducing one or both of fresh media or nutrients into the cell culture using an input conduit at a third specified rate; and
adjusting at least one of the specified rates in response to a corresponding change in another one of the specified rates to maintain a perfusion rate of the perfusion bioreactor based on a constant perfusion rate setpoint, wherein the perfusion rate defines a rate of total flow through the perfusion bioreactor such that the rate of total flow remains substantially constant when the at least one specified rate is adjusted;
wherein the input and output conduits are adjusted based on the RAMAN probe measurements of the perfusion bioreactor to maintain one or more of the process parameters within predetermined ranges, and the third specified rate of the input conduit and the first and second specified rates of each of the output conduits within respective predetermined ranges.