Patent ID: 11964274
Assignee: BIOSURFIT, SA
Field: Measurement (Instruments)
Classification: CPC B  G | IPC B  G

Claim 8:
9. A method of sequencing liquid flow in a liquid handing device, the liquid handling device having an axis of rotation about which the device can be rotated to drive liquid flow in the device, the liquid handling device including:
an upstream chamber comprising an outlet;
a downstream chamber comprising a proximal portion radially inwards of a distal portion and comprising a first port disposed in the distal portion; and
a first conduit which connects the outlet of the upstream chamber to the first port of the downstream chamber;
wherein the first conduit extends radially inwards to a crest and radially outwards from the crest to the first port of the downstream chamber; and
wherein a distance between the axis of rotation and the crest is greater than or equal to a distance between the axis of rotation and the outlet of the upstream chamber,
wherein the method comprises:
filling the first conduit from the downstream chamber to a level equal to a fill level of liquid in the downstream chamber prior to liquid filling the first conduit from the upstream chamber;
filling the first conduit from the upstream chamber to trap gas in the first conduit between respective liquids from the upstream and downstream chambers; and
rotating the device at a first rotational frequency and then a second rotational frequency greater than the first rotational frequency to vent the trapped gas into the downstream chamber and transfer liquid from the upstream chamber to the downstream chamber.