Patent ID: 11931405
Assignee: JANSSEN PHARMACEUTICA NV
Field: Pharmaceuticals (Chemistry)
Classification: CPC A  C  Y | IPC A  C

Claim 0:
1. A recombinant host cell for producing a bioconjugate of an E. coli glucosylated O4 antigen polysaccharide covalently linked to a carrier protein, wherein the glucosylated O4 antigen polysaccharide comprises the structure of Formula, wherein n is an integer of 5 to 40,
the host cell comprising:
(i) a nucleotide sequence of an rfb gene cluster for the E. coli O4 antigen polysaccharide;
(ii) a nucleotide sequence encoding a glucosyl transferase, wherein the glucosyl transferase is capable of modifying the E. coli O4 antigen polysaccharide to produce the E. coli glucosylated O4 antigen polysaccharide;
(iii) a nucleotide sequence encoding a translocase that is capable of translocating bactoprenol-linked glucose;
(iv) a nucleotide sequence encoding a glycosyltransferase that is capable of glucosylating bactoprenol;
(v) a nucleotide sequence encoding the carrier protein; and
(vi) a nucleotide sequence encoding an oligosaccharyl transferase capable of covalently linking the E. coli glucosylated O4 antigen polysaccharide to the carrier protein to produce the bioconjugate.