Patent ID: 11904011
Assignee: ZOETIS SERVICES LLC
Field: Pharmaceuticals (Chemistry)
Classification: CPC A  C | IPC A  C

Claim 0:
1. A method of reducing Porcine Reproductive and Respiratory Syndrome Virus (PRRS)-induced reproductive symptoms in a sow, the method comprising administering to said sow:
a) an isolated Porcine Reproductive and Respiratory Syndrome Virus (PRRS) wherein the protein thereof encoded by ORF1a is selected from a group consisting of those amino acid sequences that contain:
i) one, two, three or four of:
an amino acid N within the amino acid sequence AMANVYD (SEQ ID NO: 9);
an amino acid N within the amino acid sequence IGHNAVM (SEQ ID NO: 12);
an amino acid Y within the amino acid sequence CVVWYLFD (SEQ ID NO: 18); and
an amino acid H within the amino acid sequence HGVHGKY (SEQ ID NO: 21); and

ii) one or both of:
an amino acid T within the amino acid sequence PSATDTS (SEQ ID NO: 27); and
an amino acid C within the amino acid sequence APMCQDE (SEQ ID NO: 33); and

iii) one or both of:
an amino acid T within the amino acid sequence CAPTGMD (SEQ ID NO: 36); and
an amino acid A within the amino acid sequence PKVAKVS (SEQ ID NO: 39); and

iv) an amino acid N within the amino acid sequence ADFNPEK (SEQ ID NO: 45); or

b) a plasm id capable of directly transfecting a suitable host cell and expressing a Porcine Reproductive and Respiratory Syndrome Virus (PRRS) from the suitable host cell so transfected, which plasm id comprises: (1) a DNA sequence encoding an infectious RNA molecule encoding the PRRS virus, and (2) a promoter capable of transcribing said infectious RNA molecule, wherein the protein encoded by ORF1a of said virus has an amino acid sequence that contains:
(i) one, two, three, or four of
an amino acid N within the amino acid sequence ANV (see SEQ ID NO: 9);
an amino acid N within the amino acid sequence HNA (see SEQ ID NO: 12);
an amino acid Y within the amino acid sequence WYL (see SEQ ID NO: 18); and
an amino acid H within the amino acid sequence VHG (see SEQ ID NO: 21); and
(ii) one or both of

an amino acid T within the amino acid sequence ATD (see SEQ ID NO: 27); and
an amino acid C within the amino acid sequence MCQ (see SEQ ID NO: 33); and
(iii) one or both of

an amino acid T within the amino acid sequence PTG (see SEQ ID NO: 36); and
an amino acid A within the amino acid sequence VAK (see SEQ ID NO: 39); and
(4) an amino acid N within the amino acid sequence FNP (see SEQ ID NO: 45).