Patent ID: 11913070
Assignee: ARIZONA BOARD OF REGENTS ON BEHALF OF ARIZONA STATE UNIVERSITY
Field: Measurement (Instruments)
Classification: CPC C  G | IPC C  G

Claim 0:
1. A method for sequencing a polynucleotide using a bioelectronic device, the method comprising:
(a) introducing a template polynucleotide to the bioelectronic device, wherein the bioelectronic device comprises a polymerase functionally coupled to at least one of a first electrode and a second electrode;
(b) introducing a solution comprising dNTP monomers to the device comprising the template polynucleotide, each dNTP present in the solution at a pre-defined concentration; and
(c) obtaining a bioelectronic signature of polymerase activity based on current fluctuations as each complementary dNTP monomer is incorporated into the template polynucleotide;
wherein at least one characteristic of the bioelectronic signature identifies each of the complementary dNTPs incorporated into to the template polynucleotide.