Patent ID: 11905516
Assignee: ELIGO BIOSCIENCE
Field: Pharmaceuticals (Chemistry)
Classification: CPC C  A  Y | IPC A  C

Claim 6:
7. The engineered bacteriophage or packaged phagemid according to claim 1, wherein the bacteria of interest are selected from the group consisting of E. coli, E. coli STEC, Neisseria gonorrhoeae, Neisseria meningitides, Salmonella enteritidis, Streptococcus pneumoniae, Streptococcus pyogenes, Vibrio cholera, Haemophilus influenza, Klebsiella pneumoniae, Pseudomonas aeruginosa, Staphylococcus aureus, Vibrio cholera, Brucella suis, Mycobacterium tuberculosis, Enterobacter cloacae, Bacillus cereus, Corynebacterium diphtheria, Campylobacter jejuni, Listeria monocytogenes, Campylobacter coli, Bacillus thuringiensis, Yersinia enterocolitica, Helicobacter pylori, Bacteroides fragilis, Clostridium butyricum, Streptococcus agalactiae, Leuconostoc lactis, Shigella sonnei, Zymomonas mobilis, Treponema denticola, Lactobacillus plantarum, Enterococcus faecium, Bacillus liquefaciens, Staphylococcus epidermidis, Serratia marcescens, Citrobacter freundii, Parvimonas micra, Prevotella intermedia, Brucella melitensis, Aeromonas caviae, Clostridium botulinum, Clostridium perfringens, Mycoplasma pneumonia, Salmonella typhimurium, Salmonella paratyphi, Shigella flexnerii, Vibrio parahaemolyticus, Yersinia pseudotuberculosis, Actinobacter baumanii, Bacteroides distasonis, Clostridium leptum, Brevibacterium lactofermentum, Actinobacillus actinobycetemcomitans, Selnomonas ruminatium, Mycoplasma mycoides, Staphylococcus lugdunensis, Lactobacillus rhamnosus, Lactobacillus casei, Lactobacillus acidophilus, Bacillus coagulans, Pyrococcus abyssi, Selenomonas nominantium, Streptococcus fetus, Streptomyces phaechromogenes, Streptomyces ghanaenis, Enterobacter aerogenes, Morganella morganii, Fusobacterium nucleatum, Porphyromonas endodontalis, Porphyromonas gingivalis Nicrococcus luteus, Aeromonas hydrophila, Bacillus anthracis, Bartonella henselae, Bordetella pertussis, Borrelia garinii, Brucella canis, Campylobacter fetus, Clostridium tetani, Francisella tularensis, Legionella pneumophila, Leptospira interrogans, Leptospira santarosai, Leptospira weilii, Mycobacterium leprae, Shigella dysenteriae, Staphylococcus saprophyticus, Streptococcus viridans, Treponema pallidum, Ureaplasma urealyticum, Yersinia pestis and Propionibacterium acnes and the engineered bacteriophage or phagemid does not comprise, in non-coding regions and optionally in coding regions, at least one restriction site selected from the group of restriction sites consisting of the sequences SEQ ID NO: 1, SEQ ID NO: 2, SEQ ID NO: 4, SEQ ID NO: 3, SEQ ID NO: 36, SEQ ID NO: 37, SEQ ID NO: 34, SEQ ID NO: 80, GGTCTC, ACCACC, GAACC and CACAG when the group of bacteria of interest is E. coli; the sequences SEQ ID NO: 6, SEQ ID NO: 1, SEQ ID NO: 37, SEQ ID NO: 34 and SEQ ID NO: 79 when the group of bacteria of interest is E. coli STEC; the sequences SEQ ID NO: 81, SEQ ID NO: 82, GGCC, GGNNCC, GGTGA, GCCGGC, SEQ ID NO: 83, RGCGCY, CCGCGG, GCSGC, CCGG, CCACC, and AGAAA when the group of bacteria of interest is Neisseria gonorrhoeae, the sequences GGNNCC, GCGCGC, CCGG, RCCGGY, CCTTC, CCAGA, GACGC and ACACC when the group of bacteria of interest is Neisseria meningitides; the sequences SEQ ID NO: 84-88, GATCAG, GGWCC, CGGCCG and CAGAG when the group of bacteria of interest is Salmonella enteritidis; the sequence TCTAGA when the group of bacteria of interest is Streptococcus pneumoniae; the sequences SEQ ID NO: 89-91 when the group of bacteria of interest is Streptococcus pyogenes, the sequence SEQ ID NO: 02 when the group of bacteria of interest is Vibrio cholera; the sequences RGCGCY, CGCG, AAGCTT, GCGC, GTYRAC, and GTCGAC when the group of bacteria of interest is Haemophilus influenza; the sequences SEQ ID NO: 93-96 and CGCATC when the group of bacteria of interest is Klebsiella pneumoniae; the sequences SEQ ID NO: 97-99, GTCGAC, CTCGAG, CTGCAG and GGCGCC when the group of bacteria of interest is Pseudomonas aeruginosa; the sequences SEQ ID NO: 100-102, CCNGG, GCNGC and GGNCC when the group of bacteria of interest is Staphylococcus aureus; the sequence SEQ ID NO: 103 when the group of bacteria of interest is Vibrio cholera; the sequences CTCGAG and GACGAG when the group of bacteria of interest is Brucella suis; the sequences SEQ ID NO: 104 and CACGCAG when the group of bacteria of interest is Mycobacterium tuberculosis; the sequence GTCGAC when the group of bacteria of interest is Enterobacter cloacae; the sequences GCNGC, GCSGC, ACGGC and CACAG when the group of bacteria of interest is Bacillus cereus; the sequence GCGGAG when the group of bacteria of interest is Corynebacterium diphtheria; the sequences SEQ ID NO: 105-108 and GKAAYG, when the group of bacteria of interest is Campylobacter jejuni; the sequences SEQ ID NO: 109-112, TAGRAG, GTATCC and GTCGAC when the group of bacteria of interest is Listeria monocytogenes; the sequence SEQ ID NO: 113 when the group of bacteria of interest is Campylobacter coli; the sequences GGWCC and ACGGC when the group of bacteria of interest is Bacillus thuringiensis; the sequences GGCGCC and GCGCGC when the group of bacteria of interest is Yersinia enterocolitica; the sequences CCGG, GTNNAC, GTAC, CATG, TCNNGA, TGCA, GANTC, ACNGT, ACGT, CCATC, CCTC, CTNAG, GAAGA, GTSAC, CCNNGG, GAATTC, GGCC, TCNGA, CCTTC, CTGCAG and TCAG when the group of bacteria of interest is Helicobacter pylori; the sequences SEQ ID NO: 114, CCTTC, GGNNCC, GCGCGC, CCGG, RCCGGY, CCAGA, GACGC and ACACC when the group of bacteria of interest is Neisseria meningitides; the sequences SEQ ID NO: 115-121 and TCTAGA when the group of bacteria of interest is Streptococcus pneumoniae; the sequences SEQ ID NO: 89-91 and CCNGG when the group of bacteria of interest is Streptococcus pyogenes; the sequences SEQ ID NO: 92, CTGCAG, TCCGGA, GGCGCC, CTCGAG and GGNCC when the group of bacteria of interest is Vibrio cholera; the sequences SEQ ID NO: 122-124, RGCGCY, CGCG, AAGCTT, GCGC, GTYRAC, GTCGAC, CGAG, AGAAA, ACAGC and CGAAT when the group of bacteria of interest is Haemophilus influenza; the sequences SEQ ID NO: 97-99, SEQ ID NO: 125-131, GTCGAC, CTCGAG, CTGCAG, GGCGCC, ACGACC and GCCCAG when the group of bacteria of interest is Pseudomonas aeruginosa; the sequences SEQ ID NO: 100-102, CCNGG, GCNGC, GGNCC, SEQ ID NO: 132-133 and GCTGA when the group of bacteria of interest is Staphylococcus aureus; the sequences SEQ ID NO: 134-135, CTCGAG and GACGAG when the group of bacteria of interest is Brucella suis; the sequences SEQ ID NO: 104, CACGCAG and SEQ ID NO: 136 when the group of bacteria of interest is Mycobacterium tuberculosis; the sequences SEQ ID NO: 137-140, GTCGAC and ACGAAG when the group of bacteria of interest is Enterobacter cloacae; the sequences SEQ ID NO: 141-147, GCNGC, GCSGC, GCWGC, ACGGC, CTCGAG and CACAG when the group of bacteria of interest is Bacillus cereus; the sequences SEQ ID NO: 148-152 and GCGGAG when the group of bacteria of interest is Corynebacterium diphtheria; the sequences SEQ ID NO: 105-108, CATG and GKAAYG when the group of bacteria of interest is Campylobacter jejuni; the sequences SEQ ID NO: 109-110, SEQ ID NO: 112, SEQ ID NO: 153-155, TAGRAG, GTATCC, GTCGAC and ACANNNNCATC when the group of bacteria of interest is Listeria monocytogenes; the sequences SEQ ID NO: 156, GGWCC, ACGGC, GCNGC, GGWCC, ACGGC, GCNGC and CACAG when the group of bacteria of interest is Bacillus thuringiensis; the sequences GGCGCC, GCGCGC and CCGAG when the group of bacteria of interest is Yersinia enterocolitica; the sequences SEQ ID NO: 157-164, CCGG, GTNNAC, CATG, GTAC, TCNNGA, GANTC, TGCA, ACNGT, ACGT, CCATC, CCTC, CTNAG, GAAGA, CCNNGG, GTSAC, GAATTC, GGCC, TCNGA, CCTTC, CTGCAG and TCAG when the group of bacteria of interest is Helicobacter pylori; the sequences SEQ ID NO: 165 and ATGCAT when the group of bacteria of interest is Bacteroides fragilis; the sequences SEQ ID NO: 166, GCNGC and GCTNAGC when the group of bacteria of interest is Clostridium butyricum; the sequences SEQ ID NO: 167, GGNCC, GGCC and CCGG when the group of bacteria of interest is Streptococcus agalactiae; the sequences CAA TAYG, TNAGCC, CCNGG, GCNGC and CCCGC when the group of bacteria of interest is Leuconostoc lactis; the sequences SEQ ID NO: 168-169, CTGCAG, CCNGG, GACGTC, GAATTC and GTCGAC when the group of bacteria of interest is Shigella sonnei; the sequences SEQ ID NO: 170-172 and GATATC when the group of bacteria of interest is Zymomonas mobilis; the sequence SEQ ID NO: 173 when the group of bacteria of interest is Treponema denticola; the sequences SEQ ID NO: 174-75, GGNCC, CTCTTC, GCATC, GC SGC and CGRYCG when the group of bacteria of interest is Lactobacillus plantarum; the sequences SEQ ID NO: 176-177, CCGG, CCWWGG and GCWGC when the group of bacteria of interest is Enterococcus faecium; the sequences SEQ ID NO: 178-179, ATCGAT, GCWGC, GCNGC, GCGCGC, GGATC and GGGAC when the group of bacteria of interest is Bacillus liquefaciens; the sequences SEQ ID NO: 180-182, GGTGA and CCTC when the group of bacteria of interest is Staphylococcus epidermidis; the sequences SEQ ID NO: 183-186, GCNGC, CTGCAG, CCCGGG, GCCGGC, CCGCGG and CAGAG when the group of bacteria of interest is Serratia marcescens; the sequences SEQ ID NO: 187-190 and CGATCG when the group of bacteria of interest is Citrobacter freundii; the sequences SEQ ID NO: 191, GGCC, GCGATG, GTCGAC, CCGG and GCNGC when the group of bacteria of interest is Parvimonas micra; the sequences SEQ ID NO: 192-200, GGATG and GCCGGC when the group of bacteria of interest is Prevotella intermedia; the sequences SEQ ID NO: 201-202, GCWGC, TGATCA and CACAG when the group of bacteria of interest is Brucella melitensis; the sequences SEQ ID NO: 203, CTGCAG, CRRTAAG, CCGG, GTCGAC and GCYYGAC when the group of bacteria of interest is Aeromonas caviae; the sequences SEQ ID NO: 204-209, GRCGYC, GCWGC, GCSGC, CCGG and GCNGC when the group of bacteria of interest is Clostridium botulinum; the sequences SEQ ID NO: 210-211, GCNGC, GGWCC, GTCTC, GGCC, ACGGC and VGACAT when the group of bacteria of interest is Clostridium perfringens; the sequences SEQ ID NO: 212-213, CCGG, CCWWGG and GCWGC when the group of bacteria of interest is Enterococcus faecium; the sequence SEQ ID NO: 214 when the group of bacteria of interest is Mycoplasma pneumonia; the sequences SEQ ID NO: 215-217, GATCAG, CCNGG, CCWWGG and CAGAG when the group of bacteria of interest is Salmonella typhimurium; the sequences SEQ ID NO: 218-219, GGNCC, GGATG, CWTCCAG, GCSGC, GTCGAC, GTCGAC and GCATC when the group of bacteria of interest is Salmonella paratyphi; the sequences SEQ ID NO: 220, CTGCAG and GCCGGC when the group of bacteria of interest is Shigella flexnerii; the sequences SEQ ID NO: 221, CCNGG, CTCTTC and GNAATC when the group of bacteria of interest is Vibrio parahaemolyticus; the sequences SEQ ID NO: 222, CTGCAG and CGGAAG when the group of bacteria of interest is Yersinia pseudotuberculosis; the sequences SEQ ID NO: 223-230, GAAAGC and CGAGG when the group of bacteria of interest is Actinobacter baumanii; the sequences GGCGCC, GCCGGC and CACAG when the group of bacteria of interest is Bacteroides distasonis; the sequences GGNCC and GGATG when the group of bacteria of interest is Clostridium leptum; the sequences GCWGC, GCTNAGC and CACAG when the group of bacteria of interest is Brevibacterium lactofermentum; the sequences CCGG, CCGCGG, GATATC, CGGCCG and CTCGAG when the group of bacteria of interest is Actinobacillus actinobycetemcomitans; the sequences GCCGGC, CCGCGG and GAGAG when the group of bacteria of interest is Selnomonas ruminatium; the sequences TCTAGA, GGNCC, CCTC, CCATC, GANTC, CCTTC, GCATC and TGAG when the group of bacteria of interest is Mycoplasma mycoides; the sequence GGCGCC when the group of bacteria of interest is Staphylococcus lugdunensis; the sequence GATATC when the group of bacteria of interest is Lactobacillus rhamnosus; the sequences CCGCGG, CTGCAG and GATATC when the group of bacteria of interest is Lactobacillus casei; the sequence GGCC when the group of bacteria of interest is Lactobacillus acidophilus; the sequences ATCGAT, GRCGYC, GCSGC, CTGCAG, GTCTC, GGATCC, GAGCTC, CTCGAG, CACAG, GAWTC and TAAATC when the group of bacteria of interest is Bacillus coagulans; the sequences GTAC and CCGGNAG when the group of bacteria of interest is Pyrococcus abyssi; the sequences GGCCGAG and CCGG when the group of bacteria of interest is Selenomonas nominantium; the sequence CTRYAG when the group of bacteria of interest is Streptococcus fetus; the sequences CCNGG, GCATGC, TTAA, GTAC and CTAG when the group of bacteria of interest is Streptomyces phaechromogenes; the sequence GCCGGC when the group of bacteria of interest is Streptomyces ghanaenis; the sequences YGGCCR and GGNCC when the group of bacteria of interest is Enterobacter aerogenes; the sequences GGNNCC, CGATCG, TGCA and GCSGC when the group of bacteria of interest is Morganella morganii; the sequences GGCC, AAGCTT, ATGCAT and GCGC when the group of bacteria of interest is Fusobacterium nucleatum; the sequences GCAGT, GAGTC and GATATC when the group of bacteria of interest is Porphyromonas endodontalis; the sequence GAATTC when the group of bacteria of interest is Porphyromonas gingivalis; the sequences ACGCGT, GAGCTC and CAGNNNCTG when the group of bacteria of interest is Micrococcus luteus; the sequences CTCGAG, GCYYGAC, YAAMGAG and CAGCTG when the group of bacteria of interest is Aeromonas hydrophila; the sequences RTCAGG and GGWCC when the group of bacteria of interest is Bacillus anthracis; the sequence AGTACT when the group of bacteria of interest is Bartonella henselae; the sequences GGNCC, CTGCAG, GRCGYC and AGCCGCC when the group of bacteria of interest is Bordetella pertussis; the sequence CCGG when the group of bacteria of interest is Borrelia garinii; the sequences CCATC, GGCGCC, CTNAG, GGNCC and GCCGGC when the group of bacteria of interest is Brucella canis; the sequence SEQ ID NO: 37 when the group of bacteria of interest is Campylobacter fetus; the sequences CGATCG, CCCGGG and GCCGGC when the group of bacteria of interest is Clostridium tetani; the sequences GGCC and CTGCAG when the group of bacteria of interest is Francisella tularensis; the sequences CCDG and GGNCC when the group of bacteria of interest is Legionella pneumophila; the sequences GCGC and GGCC when the group of bacteria of interest is Leptospira interrogans; the sequences RGATCY, GCWGC, CCATC, GTATCC and CTAG when the group of bacteria of interest is Leptospira santarosai; the sequence GTCGAC when the group of bacteria of interest is Leptospira weilii; the sequences GGNCC, GCATC and GTCGAC when the group of bacteria of interest is Mycobacterium leprae; the sequences SEQ ID NO: 37, CCNGG, GAATTC, GGCC and GGGAC when the group of bacteria of interest is Shigella dysenteriae; the sequences GCNGC and ACGT when the group of bacteria of interest is Staphylococcus saprophyticus; the sequence GCCGGC when the group of bacteria of interest is Streptococcus viridans; the sequences GGWCC, TCGA and GMGAGC when the group of bacteria of interest is Treponema pallidum; the sequence GCNGC when the group of bacteria of interest is Ureaplasma urealyticum; and the sequences SEQ ID NO: 231-233, AGCAGY and ACCAGG when the group of bacteria of interest is Propionibacterium acnes.