Patent ID: 11910788
Assignee: REGENERON PHARMACEUTICALS, INC.
Field: Measurement (Instruments)
Classification: CPC A  C  G | IPC A  C  G

Claim 3:
4. An isolated cell obtained from a genetically modified mouse, wherein the genome of the cell and the genome of the mouse comprise a replacement of a genomic fragment comprising exons 2-7 of a mouse CD47 gene at an endogenous mouse CD47 locus with a human genomic fragment comprising exons 2-7 of a human CD47 gene to form a humanized CD47 gene,
wherein the exons of the humanized CD47 gene consist of exon 1 of the mouse CD47 gene, exons 2-7 of the human CD47 gene, and the remaining exons downstream of exon 7 of the mouse CD47 gene,
wherein exons 2-7 of the human CD47 gene encode an amino acid sequence that is at least 98% identical with the amino acid sequence as set forth in amino acids 16-292 of SEQ ID NO: 10,
wherein the humanized CD47 gene is under control of the endogenous mouse CD47 promoter at the endogenous mouse CD47 locus, and
wherein the mouse expresses a humanized CD47 protein encoded by the humanized CD47 gene.