Patent ID: 11885810
Assignee: OXFORD UNIVERSITY INNOVATION LIMITED
Field: Measurement (Instruments)
Classification: CPC G  H | IPC G  H

Claim 0:
1. A method of detecting a membrane protein by mass spectrometry, wherein the method comprises:
(a) providing a solution comprising a native membrane vesicle having a lipid bilayer to which said membrane protein is attached;
(b) sonicating the vesicle in the presence of a mass spectrometry-compatible buffer;
(c) providing a mass spectrometer comprising a nanoelectrospray ionisation source, a mass analyser and a detector;
(d) vaporising the sonicated solution using the nanoelectrospray ionisation source under conditions such that the membrane protein is released from the vesicle;
(e) ionising the membrane protein;
(f) resolving the ionised membrane protein using the mass analyser; and
(g) detecting the resolved membrane protein using the detector.