Patent ID: 11906723
Assignee: HUST-SUZHOU INSTITUTE FOR BRAINSMATICS
Field: Measurement (Instruments)
Classification: CPC G  H | IPC G  H

Claim 0:
1. A high-throughput optical sectioning three-dimensional imaging system, comprising:
a light beam modulation module configured to modulate a light beam into a modulated light beam capable of being focused on a focal plane of an objective lens and being defocused on a defocusing plane of the objective lens, wherein the light beam modulation module comprises a shaping optical path for shaping the light beam into a linear light beam and a modulation optical path for modulating the linear light beam into the modulated light beam;
an imaging camera configured to image, in different rows of pixels, at least one sample strip of at least one surface layer of a same sample under illumination of the modulated light beam to form multiple sample images, wherein the modulated light beam has incompletely identical modulated intensities on the different rows of pixels in a direction along which the different rows of pixels are arrayed, the sample strip moves in the direction along which the different rows of pixels are arrayed when imaged, and each of the different rows of pixels sequentially forms a plurality of strip image blocks in the direction along which the different rows of pixels are arrayed, the plurality of strip image blocks formed by each of the different rows of pixels are used to be stitched into a corresponding one of the sample images of the same sample strip;
a cutter configured to cut off an imaged surface layer of the sample;
a processor configured to demodulate a sample image of one sample strip of one surface layer into an optical sectioning image, and reconstruct the optical sectioning image of each sample strip of each surface layer into a three-dimensional image.