Patent ID: 11884971
Assignee: PACIFIC BIOSCIENCES OF CALIFORNIA, INC.
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 0:
1. A method of characterizing a nucleic acid, comprising
(a) extending a primer-template nucleic acid hybrid comprising a primer and a template nucleic acid with extendable nucleotide cognates and a nucleotide cognate comprising a reversible terminator moiety to produce an extended primer-template hybrid comprising the reversible terminator moiety;
(b) forming a stabilized ternary complex comprising the extended primer-template hybrid, a polymerase and a nucleotide cognate of the next base in the template nucleic acid;
(c) detecting the stabilized ternary complex to distinguish the next base;
(d) deblocking the extended primer-template hybrid;
(e) repeating steps (a) through (d) using the extended primer-template hybrid as the primer-template nucleic acid hybrid to determine a series of base multiplets in the template nucleic acid;
(f) repeating steps (a) through (e) to determine at least 3 different series of base multiplets in the template nucleic acid each time; and
(g) combining at least four different series of base multiplets to determine a sequence of the nucleic acid.