Patent ID: 11965183
Assignee: OXFORD NANOPORE TECHNOLOGIES PLC
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 20:
21. The method of claim 1, wherein
(a) at least one cysteine residue and/or at least one non-natural amino acid have been introduced into (i) the tower domain of the Dda helicase and/or (ii) the pin domain of the Dda helicase and/or (iii) the 1A (RecA-like motor) domain of the Dda helicase;
(b) at least one cysteine residue and/or at least one non-natural amino acid have been introduced into the hook domain of the Dda helicase and/or the 2A (RecA-like motor) domain of the Dda helicase; or
(c) the Dda helicase is modified to reduce its surface negative charge.