Patent ID: 11859243
Assignee: SEEGENE, INC.
Field: Computer technology (Electrical engineering)
Classification: CPC C  G | IPC C  G

Claim 5:
6. The method of claim 5, wherein RVT1D1/D2 is obtained by (i-1) incubating the first target nucleic acid sequence with the first signal-generating means for detection of the first target nucleic acid sequence, (i-2) detecting signals at the first detection temperature and the second detection temperature, and (i-3) then calculating a ratio of a signal detected at the first detection temperature to a signal detected at the second detection temperature; RVT1D2/D1 is obtained by performing the steps (i-1) and (i-2) and then calculating a ratio of the signal detected at the second detection temperature to the signal detected at the first detection temperature; RVT2D1/D2 is obtained by (ii-1) incubating the second target nucleic acid sequence with the second signal-generating means for detection of the second target nucleic acid sequence, (ii-2) detecting signals at the first detection temperature and the second detection temperature, and then (ii-3) calculating a ratio of the signal detected at the first detection temperature to the signal detected at the second detection temperature; and RVT2D2/D1 is obtained by performing the steps (ii-1) and (ii-2) and then calculating a ratio of the signal detected at the second detection temperature to the signal detected at the first detection temperature.