Patent ID: 11926858
Assignee: GLYCOM A/S
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 0:
1. A genetically modified microorganism for making an oligosaccharide or a glycoside of said oligosaccharide, comprising:
two or more recombinant genes encoding two or more glycosyl transferases which can transfer a glycosyl residue of an activated sugar nucleotide to a carbohydrate acceptor or a glycoside of said acceptor,
a biosynthetic pathway for making said activated sugar nucleotide from sucrose, and
one or more genes encoding a heterologous PTS-dependent sucrose utilization system, so that said cell is capable to use sucrose as a carbon source for making said activated sugar nucleotide and as energy source for making said oligosaccharide,, wherein the carbohydrate acceptor is not sucrose,, wherein said genetically modified microorganism is an E. coli cell of LacZ− genotype,, said oligosaccharide is lacto-N-tetraose (LNT),, said activated sugar nucleotide is UDP-Gal and UDP-GlcNAc,, said two or more glycosyl transferase comprises β-1,3-N-acetyl glucosaminyl transferase and β-1,3-galactosyl transferase,, and said heterologous PTS-dependent sucrose utilization system comprises proteins encoded by scr genes scrY for a sucrose porin, scrA for a PTS permease, scrB for a sucrose-6-phosphate hydrolase and scrR for a repressor protein.