Patent ID: 11866697
Assignee: MASSACHUSETTS INSTITUTE OF TECHNOLOGY
Field: Pharmaceuticals (Chemistry)
Classification: CPC C  A  Y | IPC A  C

Claim 0:
1. A method of modifying an Adenine in a target locus of interest, comprising delivering to said locus:
(a) a Cpf1 nickase protein;
(b) a guide molecule which comprises a guide sequence linked to a direct repeat sequence; and
(c) an adenosine deaminase protein or catalytic domain thereof;
wherein said adenosine deaminase protein or catalytic domain thereof is covalently or non-covalently linked, or is adapted to covalently or non-covalently link after delivery, to said Cpf1 nickase protein or said guide molecule;
wherein said guide molecule forms a complex with said Cpf1 nickase protein and directs said complex to bind a first DNA strand at said target locus of interest, wherein said guide sequence is capable of hybridizing with a target sequence comprising said Adenine within said first DNA strand to form a heteroduplex, wherein said guide sequence comprises a non-pairing Cytosine at a position corresponding to said Adenine resulting in an A-C mismatch in the heteroduplex formed;
wherein said Cpf1 nickase protein nicks a second DNA strand at said target locus of interest displaced by formation of said heteroduplex; and
wherein said adenosine deaminase protein or catalytic domain thereof deaminates said Adenine in said heteroduplex.