Patent ID: 11873534
Assignee: ELECTROCHAEA GMBH
Field: Biotechnology (Chemistry)
Classification: CPC C  Y | IPC C

Claim 6:
7. The method of claim 2, wherein the genotyping the one or more methanogenic microorganisms on the basis of the restriction pattern comprises detection of the presence of Methanothermobacter thermoautotrophicus DSM3590 based on at least one single nucleotide polymorphism (SNP) in the amplicon obtained from step b or step c,
wherein the SNP is part of an overlapping sequence of the first and the second recognition sequence and wherein the SNP is at a position in the PCR fragment (amplicon) amplified from the genomic nucleotide sequence of Methanothermobacter thermoautotrophicus DSM3590 by a primer pair and compared to the same position in a PCR fragment (amplicon) of a genomic nucleotide sequence of a non-Methanothermobacter thermoautotrophicus-DSM3590 Archaea, which was PCR amplified by the same primer pair;, wherein:
a.) the position of the SNP is at position 418 (SNP19) and the primer pair is SEQ ID NO: 3 (forward) and SEQ ID NO: 6 (reverse);
b.) the position of the SNP is at position 105 (SNP 11) and the primer pair is SEQ ID NO: 1 (forward) and SEQ ID NO: 4 (reverse); and
c.) the position of the SNP is at position 168 (SNP 20) and the primer pair is SEQ ID NO: 2 (forward) and SEQ ID NO: 5 (reverse) and
wherein when the SNP is SNP19, the first restriction enzyme is BamHI and the second restriction enzyme is Avail, or when the SNP is SNP11, the first restriction enzyme is SfcI and the second restriction enzyme is BstNI or ECORII or when the SNP is SNP20, the first restriction enzyme is NdeI.