Patent ID: 11948302
Assignee: NEW YORK UNIVERSITY
Field: Measurement (Instruments)
Classification: CPC G | IPC G

Claim 0:
1. A method of characterizing a parameter of a viral protein by holographic microscopy, comprising the steps of:
receiving holographic image data having a set of concentric bright and dark rings associated with a functionalized probe bead;
determining by Lorenz-Mie analysis an effective sphere estimate of each of the functionalized probe bead's effective sphere diameter, and effective sphere refractive index; and
determining a diameter difference between the effective sphere diameter with a predetermined diameter for unbound functionalized probe beads, with diameter difference representing the result of material bound to the bead and medium within the effective sphere estimate, wherein the effective sphere diameter (dp) is equal to

dp=d0+2δf 

wherein dp is the effective sphere diameter, d0 is the predetermined diameter for unbound functionalized probe beads, δ is effective optical thickness of a complete layer of the viral protein, and f is the fraction of binding sites on the functionalized probe bead.