Patent ID: 11965888
Assignee: DEPUY SYNTHES PRODUCTS, INC.
Field: Measurement (Instruments)
Classification: CPC G  C | IPC C  G

Claim 0:
1. A method for quantifying endotoxin concentration in a biofilm sample comprising cellulose from a gram negative bacteria comprising:
digesting the gram negative biofilm sample with an enzyme for the digestion of extracellular matrix of the gram negative bacteria, wherein the enzyme comprises cellulose, to form a digested biofilm sample;
separating the digested biofilm sample into a supernatant and a cell pellet;
combining the cell pellet with a cell lysing agent to release endotoxins and form an endotoxin suspension;
concentrating the endotoxin suspension into an endotoxin sample and a suspension supernatant; and,
performing a bacterial endotoxin test (BET) assay on the endotoxin sample to obtain a sample endotoxin value.