Patent ID: 11884918
Assignee: SYNTHEGO CORPORATION
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 5:
6. The CRISPR OFF polynucleotide of claim 1(c), wherein the photocleavable linker comprises a compound of Formula:, , wherein:
R1, R2, R3, R4, and R5 are each independently selected from H, alkyl, substituted alkyl, alkoxy, alkenyl, alkynyl, haloalkyl, haloalkoxy, alkoxyalkyl, amino, aminoalkyl, halo, cyano, hydroxy, hydroxyalkyl, heteroalkyl, C-carboxy, O-carboxy, C-amido, N-amido, nitro, sulfonyl, sulfo, sulfino, sulfonate, S-sulfonamido, N-sulfonamido, optionally substituted carbocyclyl, optionally substituted aryl, optionally substituted heteroaryl and optionally substituted heterocyclyl;
alternatively, two or more of R1, R2, R3, and R4, together with the atoms to which they are attached form a ring or ring system selected from optionally substituted 5- to 10-membered heteroaryl, optionally substituted 5- to 10-membered heterocyclyl, and optionally substituted C5-10 carbocycle; and
m is an integer selected from 2 to 10; and
X is selected from O, S, or dicyanomethylene; and
* indicates a point of attachment to H, or a first nucleotide; and
** indicates a point of attachment to OH, or a second nucleotide.