Patent ID: 11859238
Assignee: GEN-PROBE INCORPORATED
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 25:
26. A method of amplifying a target nucleic acid sequence present in a sample, the method comprising the steps of:
(a) performing a first phase linear amplification reaction that amplifies the target nucleic acid sequence under conditions that do not support exponential amplification thereof,
wherein the first phase linear amplification reaction generates a first amplification product;

(b) performing a second phase amplification reaction under conditions allowing exponential amplification of the first amplification product, thereby generating a second amplification product that comprises the target nucleic acid sequence or the complement thereof; and
c) detecting the second amplification product while the second phase amplification reaction is occurring,
wherein step (c) comprises detecting with a fluorescently labeled hybridization probe.