Patent ID: 11930809
Assignee: LIFELINE SCIENTIFIC, INC.
Field: Biotechnology (Chemistry)
Classification: CPC A  C | IPC A  C

Claim 9:
10. A method for storing a biomaterial comprising:
preparing a composition by placing a biomaterial in a solution that includes at least one agent that reduces or prevents a loss of biomaterial properties, the biomaterial properties comprising cell viability and extracellular matrix integrity, the extracellular matrix integrity including extracellular matrix permeability;
after formation of the composition, adjusting a temperature of the solution comprising the biomaterial to a hypothermic temperature ranging from −25° C. to +10° C.; and
then reducing or preventing the loss of the cell viability and the extracellular matrix permeability by storing the biomaterial in the solution at a hypothermic temperature ranging from 0° C. to +5° C. for a storage period of hours to 1 month,
wherein the solution is an animal product-free solution,
the biomaterial comprises chondrocytes in an extracellular matrix or cartilage, and
the at least one agent comprises doxycycline having a concentration ranging from 10 μM to 30 μM.