Patent ID: 11946073
Assignee: HEALTHINA STEM CELL INDUSTRY PLATFORM (TIANJIN) LIMITED
Field: Pharmaceuticals (Chemistry)
Classification: CPC C  A | IPC A  C

Claim 0:
1. A method for non-enzymatic 3D culture and amplification of mesenchymal stem cells (MSCs), comprising the following steps:
S1) preparing poly(lactic-co-glycolic acid) (PLGA) porous microspheres, wherein preparing the PLGA porous microspheres comprises the following steps:
S1a) dissolving PLGA, cholesterol, and NH3HCO3 in ether at a weight ratio of (10:1:1)-(10:2:2) at a room temperature to obtain an ether mixture,
adding water to the ether mixture, wherein a volume ratio of the water added to the ether is (3:1)-(4:1), and
performing ultrasonication for 15-40 min to form a water-in-oil (W/O) emulsion; and
S1b) performing spray drying on the W/O emulsion obtained in step (Sla) by a spray dryer to form the PLGA porous microspheres;
S2) preparing a PLGA-PEG-PLGA thermosensitive coating microcarrier, wherein PEG is polyethylene glycol;
S3) culturing and amplifying the MSCs; and
S4) performing non-enzymatic separation of the MSCs: reducing a culture temperature to below a critical phase transition temperature, and centrifuging a culture medium to collect the MSCs.