Patent ID: 11898183
Assignee: nan
Field: Pharmaceuticals (Chemistry)
Classification: CPC C  A  Y | IPC A  C

Claim 16:
17. A method for constructing the recombinant microbe as claimed in claim 2, said method comprising: (a) obtaining one or more recombinant vector, said recombinant vector encoding a repertoire of genes encoding phenyl alanine ammonia-lyase (PAL), Cinnamate-4-hydroxylate (C4H), 4-coumaroyl CoA-ligase (4CL), hydroxycinnamoyl-CoA quinate hydroxycinnamoyltransferase (HCT), p-coumaroyl quinate 3′-hydroxylase (C3H), caffeoyl CoA O-methyltransferase (CCoAOMT), bifunctional pinoresinol-lariciresinol reductase (DIRPLR), secoisolariciresinol dehydrogenase (SDH), O-methyltransferase (OMT), 2-oxoglutarate/Fe(II)-dependent dioxygenase (2-ODD), 2-Deoxy-d-ribose-5-phosphate aldolase, Cytochrome P450 oxidoreductase CYP719, Cytochrome P450 oxidoreductase CYP71, Cytochrome P450 oxidoreductase CYP82D, UDP glucosyl transferase, at least one gene encoding a protein transporter selected from the group consisting of ATP-Binding Cassette (ABC) transporter, Major Facilitator Superfamily (MFS) transporters, SMR (small multidrug resistant) family, RND (Resistance-Nodulation-Cell Division) family, and the MATE (multidrug and toxic compound extrusion) family, and at least one regulatory circuit selected from the group consisting of nitric oxide (NO) operon, arabinose (AraC) operon, fumarate and nitrate reductase (FNR) operon, thiosulphate-responsive regulatory circuit, and tetrathionate-responsive regulatory circuit; and (b) transforming a host microbe with the recombinant vector obtained in step (a), to obtain the recombinant microbe as claimed in claim 2.