Patent ID: 11951474
Assignee: LIFE TECHNOLOGIES CORPORATION
Field: Measurement (Instruments)
Classification: CPC B  C  G  Y | IPC B  C  G

Claim 14:
15. An apparatus comprising:
a reagent source;
one or more wash solution sources;
a flowcell including a fluidics interface member mounted on and sealingly attached to a housing, the flowcell defining a plurality of separate flow chambers, each flow chamber having an inlet and an outlet, the inlet in fluid communication with the reagent source and the outlet connected to waste, each flow chamber having interior walls formed by attachment of the fluidics interface member to the housing;
the reagent source to provide a nucleotide solution to the flowcell; and
for each flow chamber of the plurality of separate flow chambers:
a passage in fluid communication with the inlet;
a junction in fluid communication with the passage, the reagent source, and a wash solution source of the one or more wash solution sources;
a valve to control passage of a wash solution to the junction; and

a fluidic circuit comprising:
a node having a fluid outlet;
a plurality of fluid inlets, each fluid inlet of the plurality of fluid inlets uniquely associated with a respective reagent source of a plurality of reagent sources, the plurality of reagent sources comprising the reagent source;
at least one waste port;
a plurality of passages, each passage of the plurality of passages providing a distinct fluid pathway between the node and the at least one waste port, each passage of the plurality of passages including a junction to a respective inlet passage providing fluid communication between the each passage and a respective fluid inlet of the plurality of fluid inlets;
an outlet passage in fluid communication with the node through the fluid outlet and in fluid communication with the reagent source; and
a wash fluid inlet in fluid communication with the outlet passage.