Patent ID: 11971403
Assignee: OIL CROPS RESEARCH INSTITUTE, CHINESE ACADEMY OF AGRICULTURAL SCIENCES
Field: Measurement (Instruments)
Classification: CPC G | IPC G

Claim 0:
1. A time-resolved fluorescence kit for synchronously detecting 4,15-diacetoxyscirpenol, aflatoxin B1 and sterigmatocystin, comprising an immunochromatography time-resolved fluorescence test strip and a sample reaction bottle containing an europium-labeled anti-4,15-diacetoxyscirpenol monoclonal antibody, an europium-labeled anti-aflatoxin B1 monoclonal antibody and an europium-labeled anti-sterigmatocystin monoclonal antibody, wherein the immunochromatography time-resolved fluorescence test strip comprises a liner, wherein a water absorption pad, a detection pad and a sample pad are sequentially attached to one side of the liner from top to bottom, adjacent pads are connected in an overlapping manner at a joint, the detection pad uses a nitrocellulose membrane as a base pad, a quality control line and detection lines are transversely arranged on the nitrocellulose membrane from top to bottom, the quality control line is coated with a rabbit antimouse polyclonal antibody, the three detection lines are located below the quality control line, and the detection lines are coated with a 4,15-diacetoxyscirpenol-bovine serum albumin conjugate, an aflatoxin B1-bovine serum albumin conjugate and a sterigmatocystin-bovine serum albumin conjugate respectively; and the anti-4,15-diacetoxyscirpenol monoclonal antibody is secreted by a hybridoma cell strain DAS5G11E7 with the preservation number of CCTCC NO: C201881,
wherein the anti-aflatoxin B1 monoclonal antibody is secreted by a hybridoma cell strain 1C11 with the preservation number of CCTCC NO. C201013; and the anti-sterigmatocystin monoclonal antibody is secreted by a hybridoma cell strain ST03 with the preservation number of CCTCC NO. C2013187.