Patent ID: 11939573
Assignee: BIO-RAD LABORATORIES, INC.
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 0:
1. A method of capturing cells with specific cell surface markers and analyzing polynucleotides from the cells, the method comprising,
providing antibodies associated with a unique antibody-specific barcode sequence followed by;
coating cells with the antibodies such that the antibodies bind to target proteins on the cells to form antibody-coated cells followed by;
capturing (i) the antibody-coated cells and (ii) first droplets containing cell-specific barcode adaptors in second droplets followed by;
fusing contents of the first droplet with contents of the second droplet; and
lysing the cells in the second droplets followed by;
tagging polynucleotides in the second droplets with the cell-specific barcode adaptors to form tagged polynucleotides followed by;
sequencing the tagged polynucleotides, wherein adaptor sequences in sequencing reads can be used to infer which reads came from which cell.