Patent ID: 11965813
Assignee: CYTEK BIOSCIENCES, INC.
Field: Measurement (Instruments)
Classification: CPC G | IPC G

Claim 0:
1. A cytometer fluidics system, comprising:
a sheath tank;
a manifold assembly coupled to the sheath tank via a first sheath line;
a flow cell coupled to the manifold assembly via a second sheath line;
a first valve coupled to the first sheath line and in the manifold assembly;
a start stream path including the first sheath line and the second sheath line, wherein the start stream path extends from the sheath tank to the flow cell;
a pressure regulation system coupled to the sheath tank, the pressure regulation system including a pressure regulator and a transducer coupled to the first valve and electrically coupled to the pressure regulator, wherein the pressure regulator is an only regulated pressure source in the cytometer fluidics system that applies a regulated pressure to the sheath tank to maintain a total flow rate of fluids in the cytometer fluidics system, wherein the transducer is configured to sense measured pressure at the first valve and convert the measured pressure to a voltage and the pressure regulator is configured to receive the voltage from the transducer and convert the voltage into the regulated pressure;
wherein the pressure regulation system maintains the regulated pressure by attempting to match the regulated pressure with the measured pressure;
wherein the total flow rate is equal to a flow rate of sheath fluid in the start stream.