Patent ID: 11859175
Assignee: STEMON INC.
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 0:
1. A cell reprogramming method comprising:
subjecting a mixture of human dermal fibroblasts (HDF) and a culture medium to ultrasonic waves treatment having an output intensity of 0.5 W/cm2 to 3 W/cm2 for 1 to 5 seconds which promote an environmental influx for a cell reprogramming, wherein the ultrasonic waves treatment induces damage of the cell membrane which is recovered 2 hours after being subjected to the ultrasonic waves treatment; and
culturing, immediately after the ultrasonic waves treatment, the mixture subjected to the ultrasonic waves treatment for a predetermined time to obtain reprogrammed cells,
wherein the culture medium is any one of a hepatocyte differentiation-inducing medium, an adipocyte differentiation-inducing medium, or a neuronal cell differentiation-inducing medium,
wherein the ultrasonic treatment promotes the environmental influx without introduction of a reprogramming inducing factor other than any one of the differentiation-inducing medium,
wherein the reprogrammed cells are any one of neurons expressing any one of PAX6, Nestin, Sox1, Sox2, MAP2, TuJl, GFAP or O4; hepatocytes expressing any one of AFP, HNF1a, HNF4a, CK18 or ALB; or adipocytes stained with oil red O and expressing any one of Pparc2, C/ebpa, aP2 or Fabp4.