Patent ID: 11920147
Assignee: CASE WESTERN RESERVE UNIVERSITY
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC A  C

Claim 10:
11. A method of increasing the transduction efficiency of an HIV-1 lentiviral vector construct comprising:
inserting a genomic RNA packaging enhancer (GRPE) element into an HIV-1 lentiviral vector construct, the GRPE element comprising a nucleic acid sequence encoding the P2 and P3 stem loops in the gag p1-p6 domain of the HIV-1 RNA wild-type genome, wherein the GRPE element maintains P2 and P3 stem loop structure and includes two or fewer nucleotide substitutions in the nucleic acid sequence encoding the P2 and P3 stem loops in the gag p1-p6 domain of the HIV-1 RNA wild-type genome, the HIV-1 lentiviral vector construct comprising HIV-1 lentiviral nucleic acid sequences sufficient for reverse transcription and packaging, wherein the HIV-1 lentiviral nucleic acid sequences are devoid of a gag matrix/capsid/nucleocapsid (MA/CA/NC) coding sequence; and
administering the HIV-1 lentiviral vector construct including the inserted GRPE element to a first cell, wherein the presence of the GRPE element increases the HIV-1 genomic RNA packaging in recombinant HIV-1 particles released from the first cell, wherein the increased HIV-1 genomic RNA packaging is positively correlated to an increase in the transduction efficiency of the recombinant HIV-1 particles when the recombinant HIV-1 particles are administered to a second cell.