Patent ID: 11859245
Assignee: INSTITUTE OF ZOOLOGY, CHINESE ACADEMY OF SCIENCES
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 6:
7. The method of claim 6, wherein in 1), obtaining the cell lysate solution comprises: suspending cells of the biological sample in a phosphate buffer saline (PBS) solution, centrifuging the PBS solution comprising the cells at 500×g for 3 min, removing a supernatant of the PBS solution and repeating the centrifuging and removing the supernatant for 3 times; resuspending the cells in an isotonic buffer, dispersing the cells, adding the cells to 2 volumes of CHAPS lysis buffer with respect to a volume of the cells, lysing the cells on an ice for 30 min, centrifuging the cells at 16000×g for 20 min, collecting a supernatant and/or storing the supernatant at −80° C.