Patent ID: 11939363
Assignee: ARCTURUS THERAPEUTICS, INC.
Field: Pharmaceuticals (Chemistry)
Classification: CPC C  A | IPC A  C

Claim 17:
18. A composition comprising a DNA template encoding a modified mRNA, wherein
(i) the modified mRNA comprises a coding region of a wild-type mRNA that is expressible, wherein
(a) one or more codons of the coding region have been replaced such that the occurrence of uridine monomers in the coding region is reduced by at least 20% as compared to the coding region of the wild-type mRNA; and
(b) the modified mRNA encodes a polypeptide or protein; and

(ii) the modified mRNA contains one or more 5-methoxyuridines,, wherein the composition comprises an at least three-fold lower level of double stranded RNA as compared to a composition comprising an mRNA having the same coding region sequence with a similarly reduced occurrence of uridine monomers in the coding region as the modified mRNA and lacking 5-methoxyuridine, and, wherein the composition is a synthesis mixture.