Patent ID: 11946098
Assignee: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA
Field: Measurement (Instruments)
Classification: CPC C  G  A | IPC A  C  G

Claim 1:
2. The method of claim 1, wherein:
each recognition element reversibly binds the target species at a known equilibrium dissociation constant value;
the minimally responsive pulse frequency is a frequency which results in a sensor output that is substantially independent of the concentration of the target species in the sample; and
the concentration of the target species, [T] is calculated by an equation, [
   T
   ]
  
  =
  
   
    K
    D
   
   ⁢
   
    
     i
     -
     
      α
      ⁢
      
       i
       NR
      
     
    
    
     
      γα
      ⁢
      
       i
       NR
      
     
     -
     i
    
   
  
 

wherein i is the measured output value at the responsive pulse frequency;
wherein iNR is the measured output value at the minimally responsive pulse frequency;
wherein the dissociation constant value is denoted KD;
wherein γ is a constant comprising the ratio of target-saturated output signal to target-free output signal at the responsive pulse frequency for sensors of the selected design operated under the selected assay conditions; and
wherein α is a constant comprising the ratio of iNR output signal at the minimally responsive frequency to target-free output signal at the responsive frequency for sensors of the selected design operated under the selected assay conditions.