Patent ID: 11906432
Assignee: ESSENLIX CORPORATION
Field: Measurement (Instruments)
Classification: CPC G | IPC G

Claim 83:
84. The method of claim 80, further comprising an optical enhancer,
wherein the first and second plates are configured to sandwich the sample, the detection probe, the permeabilization agent, and the optical enhancer between the two sample contact areas of the two plates to form a thin layer of a thickness of 200 microns (μm) or less;
wherein the optical enhancer diffuses in the sample, binds to the cell, and is capable of emitting a light of a wavelength that overlaps with or within a 150 nm from the wavelength of the light emitted by the detection probe;
wherein the thin layer sample thickness is configured so that for a given concentration of the cell in the sample, each individual cell does not substantially overlap other cells in an imaging, wherein the imaging is performed without a washing step; wherein the thickness of the thin layer, the concentration of the detection probe in the sample, or the concentration of the optical enhancer in the sample is configured to make, in the step (e) of imaging, in the thin layer, the location having the bound detection probe is distinguishable from the locations not having the bound detection probe, wherein the bound detection probe is the detection probe bound to the analyte in the cell; and
wherein the washing step is a step of removing unbound detection probe, permeabilization reagent, or both from the sample contact areas.