Patent ID: 11970719
Assignee: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 0:
1. A method of guiding a CasY protein to a target sequence of a target nucleic acid,
the method comprising contacting the target nucleic acid with an engineered and/or non-naturally occurring complex comprising:
(a) a CasY protein;
(b) a CasY guide RNA that comprises a guide sequence that hybridizes to a target sequence of the target nucleic acid, and comprises a region that binds to the CasY protein; and
(c) a CasY transactivating noncoding RNA (CasYtrancRNA);, wherein:
(i) the CasY protein comprises an amino acid sequence having 95% or more identity with the CasYI sequence of SEQ ID NO: 1, and the CasY trancRNA comprises a nucleotide sequence having 98% or more identity with the CasYI trancRNA sequence of SEQ ID NO: 245; or
(ii) the CasY protein comprises an amino acid sequence having 95% or more identity with the CasY2 sequence of SEQ ID NO: 2, and the CasYtrancRNA comprises a nucleotide sequence having 98% or more identity with the CasY2 trancRNA sequence of SEQ ID NO: 246; or
(iii) the CasY protein comprises an amino acid sequence having 95% or more identity with the CasY4 sequence of SEQ ID NO: 4, and the CasYtrancRNA comprises a nucleotide sequence having 98% or more identity with the CasY4 trancRNA sequence of SEQ ID NO: 247.