Patent ID: 11972620
Assignee: nan
Field: Measurement (Instruments)
Classification: CPC G | IPC G

Claim 9:
10. A non-transitory computer-readable medium having instructions stored thereon that are executable by a computing device to perform operations comprising:
(A) select a first image from a plurality of images of a moving cell in a sample acquired by an imaging flow cytometer, the plurality of images being acquired across multiple different imaging modes including a side scatter image and a fluorescence image;
(B) segment the first image into subcomponents representing parts of the moving cell;
(C) segment a second image from the plurality of images of the moving cell into at least one subcomponent representing a part of the moving cell to generate a subcomponent mask, wherein the second image is of a different imaging mode than the first image;
(D) spatially correlate the subcomponent mask to the segmented first image;
(E) apply a graph cut segmentation to the second image using the subcomponent mask as a foreground object marker for the segmented first image to generate improved image data having increased location accuracy of the subcomponents; and
(F) reprocess the first image using the image data generated in (E) to identify cell morphology of the moving cell.