Patent ID: 11919006
Assignee: INDIAN INSTITUTE OF TECHNOLOGY, KHARAGPUR
Field: Measurement (Instruments)
Classification: CPC B  G  C | IPC B  C  G

Claim 11:
12. The method as claimed in claim 1 further comprising carrying out in said microcontroller based isothermal heating unit microcontroller-controlled isothermal heating of at least one reaction microchamber containing said RT LAMP reaction mixture and complementary DNA probe selected from SEQ ID NO:8 for ORF1B, SEQ ID NO:17 for N gene, and SEQ ID NO:26 for E gene thereby integrating said initial RT LAMP reaction for reverse transcription of the pathogenic RNA into cDNA to generate amplified 5′-biotinylated labelled target DNA; and subsequently, conducting said hybridization of the thus amplified 5′-biotinylated labelled target DNA, so as to concomitantly execute harmonically synchronized reverse transcription of RNA, amplification of the resulting c-DNA, and specific DNA-probe hybridization of the resulting products as a single operative preprogrammed user-step based close tube reaction to avoid any intermediate user intervention and hence minimize carry over contamination effects despite being executable outside controlled laboratory environment, with gains in the accuracy of the test outcome via operative inclusion of the specific probing step.