Patent ID: 11920193
Assignee: OXFORD NANOPORE TECHNOLOGIES PLC
Field: Measurement (Instruments)
Classification: CPC C  G | IPC C  G

Claim 0:
1. A method of characterizing a polynucleotide, the method comprising:
(i) combining in a solution:
a) a construct comprising a double-stranded polynucleotide, having a template strand and a complement strand, wherein the template strand and the complement strand are not covalently linked, with
b) a detector disposed in a membrane, wherein at least one tag that binds to the complement strand is conjugated to the detector; and
c) a polymerase and nucleotides;

(ii) processing the template strand using the polymerase to produce a product and by-products, the product comprising a third polynucleotide strand complementary to the template strand, wherein as the template strand of the double-stranded polynucleotide is processed, the complement strand becomes bound to the detector via the at least one tag conjugated to the detector; and
(iii) detecting the by-products through addition of each nucleotide to the third polynucleotide strand by the polymerase.