Patent ID: 11971357
Assignee: PRESIDENT AND FELLOWS OF HARVARD COLLEGE
Field: Measurement (Instruments)
Classification: CPC G | IPC G

Claim 4:
5. A method for measuring a composition of a biological sample, the method comprising:
receiving a stimulated Raman scattering (SRS) image of the biological sample from an optical detector;
via a processor computationally removing the effect of light scattering in the received SRS image by measuring attenuation of the received SRS image and applying an attenuation factor via a normalization mask to the SRS image;
determining an absolute concentration of total protein, total lipid, and/or water from the biological sample via a processor;
acquiring SRS images at selected Raman bands;
determining an SRS intensity of each of the selected Raman bands; and
mapping the determined SRS intensity of the each of the selected Raman bands to a corresponding protein, lipid, or water fraction.