Patent ID: 11879131
Assignee: SOUTH CHINA AGRICULTURAL UNIVERSITY
Field: Biotechnology (Chemistry)
Classification: CPC C  Y | IPC C

Claim 0:
1. A method for improving lodging resistance of a Zea mays plant comprising:
(i) constructing a recombinant plant expression vector carrying a ZmSBP12 coding sequence as set forth in SEQ ID NO: 2 or SEQ ID NO: 6 which encodes the ZmSBP12 protein as set forth in SEQ ID NO:1, wherein said recombinant plant expression vector comprises inserting the nucleotide sequence as set forth in SEQ ID NO: 2 or SEQ ID NO: 6 between Pstl and BamHI restriction sites of a modified pCAMBIA vector to obtain a ZmSBP12 overexpression vector pCAMBIA-Ub1::ZmSBP12-eGFP;
(ii) transforming said constructed recombinant plant expression vector into a Zea mays plant tissue or a Zea mays plant cell;
(iii) overexpressing said ZmSBP12 protein in the transformed Zea mays plant tissue or the Zea mays plant cell;
(iv) regenerating transformed Zea mays plants overexpressing said ZmSBP12 protein from said transformed Zea mays plant tissue or the Zea mays plant cell of step (iii); and
(v) selecting a transformed Zea mays plant from said regenerated transformed plants of step (iv) that overexpresses said ZmSBP12 protein and exhibits increased lodging resistance as compared to a control or wild-type Zea mays plant that is not transformed with said constructed recombinant plant expression vector and is grown under identical growth conditions.