Patent ID: 11890339
Assignee: THE UNITED STATES OF AMERICA, AS REPRESENTED BY THE SECRETARY, DEPARTMENT OF HEALTH AND HUMAN SERVICES
Field: Pharmaceuticals (Chemistry)
Classification: CPC A  C | IPC A  C

Claim 0:
1. An immunogen, comprising:
a recombinant Nipah virus (NiV) F ectodomain trimer stabilized in a prefusion conformation by one or more amino acid substitutions in protomers of the trimer, the amino acid substitutions comprising one or more of the following:
cysteine substitutions at NiV F positions 104 and 114 that form a non-natural intra-protomer disulfide bond, or cysteine substitutions at NiV F positions 114 and 426 that form a non-natural intra-protomer disulfide bond;
a proline substitution at NiV F position 191;
a phenylalanine substitution at NiV F position 172;
a glycine substitution at NiV F position 70; and
a deletion of NiV F positions 102-113 with positions 101 and 114 linked by a glycine-serine linker;
wherein the NiV F positions are according to the reference NiV F sequence set forth as SEQ ID NO: 52.