Patent ID: 11965002
Assignee: THE BD OF TRUSTEES OF THE UNIVERSITY OF ILLINOIS
Field: Pharmaceuticals (Chemistry)
Classification: CPC C  A | IPC A  C

Claim 29:
30. A fusion protein comprising a Cre recombinase having inserted in between amino acid residues 59-60 of the Cre recombinase, a light-responsive construct comprising a first photoreceptive polypeptide domain and a second photoreceptive polypeptide domain, wherein said first and second photoreceptive polypeptide domains are the same and:
(i) are operatively-linked via a flexible linker;
(ii) comprise
(a) amino acid residues 37-184 of SEQ ID NO:1,
(b) amino acid residues 40-186 of SEQ ID NO:1,
(c) a variant of (a) or (b) comprising one or more amino acid substitutions selected from the group consisting of Ile74Val, Ile85Val, Met135Ile, and Met165Ile, or
(d) a variant of amino acids 37-186 of SEQ ID NO:1 comprising one or more amino acid substitutions selected from the group consisting of Ile74Val, Ile85Val, Met135Ile, and Met165Ile; and
(iii) homodimerize with each other.