Patent ID: 11898179
Assignee: PRESIDENT AND FELLOWS OF HARVARD COLLEGE
Field: Pharmaceuticals (Chemistry)
Classification: CPC C  A | IPC A  C

Claim 27:
28. A method of editing a polynucleotide encoding an ion channel in a dorsal root ganglion (DRG) neuron, the method comprising contacting the ion channel-encoding polynucleotide with:
(i) a fusion protein comprising: (a) a guide nucleotide sequence-programmable DNA binding protein domain; and (b) a cytosine deaminase domain, wherein the fusion protein further comprises a uracil glycosylase inhibitor (UGI) domain; and
(ii) a guide nucleic acid molecule targeting the fusion protein of (i) to a target cytosine (C) base in the ion channel-encoding polynucleotide, wherein the guide nucleic acid molecule is selected from SEQ ID NOs: 834-1125 or 1273-1287;
whereby the contacting results in deamination of the target C base by the fusion protein, resulting in a cytosine (C) to thymine (T) change in the ion channel-encoding polynucleotide.