Patent ID: 11944655
Assignee: ALPINA PRODUCTOS ALIMENTICIOS S.A.S.—BIC
Field: Pharmaceuticals (Chemistry)
Classification: CPC A | IPC A

Claim 7:
8. A process for the encapsulation of probiotics comprising:
a) mixing a sporulated probiotic with a melted or liquid-phase lipid wall material to obtain a solid lipid sphere;
b) mixing a protein material, a carbohydrate and water until a protein film is formed;
c) homogenizing the protein film; and
d) fluidizing the solid lipid sphere of step (a), and spraying the protein film of step (c) on the solid lipid sphere,
wherein the solid lipid sphere comprises between 0.1 and 3% of a sporulated probiotic; and between 97 and 99.9% of a lipid wall material, both based on the total weight of the solid lipid sphere;
wherein the protein film comprises between 5 and 25% of a protein material; between 20 and 35% of a carbohydrate; and between 55 and 75% of water, based on the total weight of the protein film;
wherein said sporulated probiotic is selected from the group consisting of Bacillus coagulans, Bacillus subtilis, Bacillus clausii, Bacillus cereus, Bacillus licheniformis and mixtures thereof;
wherein said lipid wall material is selected from the group consisting of beeswax, palm kernel oil, palm oil, coconut oil, palm stearin, peanut butter, and mixtures thereof;
wherein said protein material is selected from the group consisting of milk protein, whey, egg white, sodium caseinate, gelatin, and mixtures thereof.