Patent ID: 11873474
Assignee: WILSON WOLF MANUFACTURING
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 1:
2. The method of claim 1 wherein after said second flow control component has terminated the flow of fluid through said cell removal conduit, the method further comprising;
venting said cell culture device to atmosphere, said first flow control component is opened to allow the flow of fluid through said media removal conduit, liquid media is moved through said media removal conduit into said cell culture device, said first flow control component is closed, and said cell culture device is no longer vented to atmosphere, and
delivering gas from said gas delivery component into said cell culture device to displace liquid media and cells from said cell culture device into a cell collection vessel connected to said cell removal conduit, said second fluid detection component determining when the liquid media that is moving through said cell removal conduit is replaced by gas and upon making that determination said second fluid detection component sends a signal to said second fluid flow control component and upon receiving said signal, said second flow control component terminates the flow of fluid through said cell removal conduit.