Patent ID: 11946865
Assignee: BECTON, DICKINSON AND COMPANY
Field: Measurement (Instruments)
Classification: CPC G  A  C | IPC A  C  G

Claim 0:
1. A method of determining the presence of an analyte of interest in a blood sample, the method comprising:
introducing a reference fluid into a reference vial comprising a reference vial sensor comprising a fluorophore and a pH indicator;
transmitting light at an excitation frequency of the reference vial sensor to the reference vial to cause the reference vial sensor to emit a reference vial fluorescence signal;
filtering the reference vial fluorescence signal using a first emission filter and a second emission filter that is different than the first emission filter;
measuring an intensity of the reference vial fluorescence signal that was filtered using the first emission filter and an intensity of the reference vial fluorescence signal that was filtered using the second emission filter to generate a reference ratio;
inoculating a blood culture test vial comprising a test vial sensor with the blood sample, the test vial sensor comprising a fluorophore and a pH indicator;
transmitting light at an excitation frequency of the test vial sensor to the test vial to cause the test vial sensor to emit a test vial fluorescence signal;
filtering the test vial fluorescence signal using the first emission filter and the second emission filter;
measuring an intensity of the test vial fluorescence signal that was filtered using the first emission filter and an intensity of the test vial fluorescence signal that was filtered using the second emission filter to generate a test vial ratio;
determining a pH state of the pH indicator of the test vial sensor by comparing the test vial ratio to the reference ratio, the pH state indicative of a pH of the blood sample; and
determining the presence of the analyte of interest based on the determined pH state of the pH indicator of the test vial sensor, the analyte of interest comprising a microorganism.