Patent ID: 11970700
Assignee: INTRON BIOTECHNOLOGY, INC.
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 5:
6. A method for RNA molecule synthesis comprising: RNA molecule synthesis comprising:
introducing, to a cell, a plasmid comprising the arrangement in order of a) a 5′-untranslated region (UTR) sequence, b) a translation initiation codon ATG, c) an enhancing genetic element DNA sequence set forth in SEQ ID NO: 1, d) a target protein-coding sequence placed adjacent to 3′-end of the said enhancing genetic element DNA sequence set forth in SEQ ID NO: 1, e) a 3′-untranslated region (UTR) sequence, and f) a poly A sequence, and
incubating the cell for sufficient time to allow for in vitro transcription of a RNA molecule transcribed from the plasmid, thereby synthesizing the RNA molecule.