Patent ID: 11891647
Assignee: NATIVE MICROBIALS, INC.
Field: Computer technology (Electrical engineering)
Classification: CPC C  G | IPC C  G

Claim 0:
1. A method of forming an active microorganism bioensemble of active microorganism strains configured to alter a property in a target biological environment, comprising:
obtaining;
an identified sample;
detecting presence of a plurality of microorganism strains in the identified sample;
determining a total quantity of microorganisms from the plurality of microorganism strains;
identifying, by-sequencing DNA in each sample a set of DNA representing whole genomes of the plurality of microorganism strains, each DNA representing a whole genome in the set of DNA representing whole genomes corresponding to a microorganism strain from the plurality of microorganism strains in the identified sample;
obtaining a set of sequencing reads associated with the plurality of microorganism strains from sequencing the DNA in the identified sample;
comparing each sequencing read from the set of sequencing reads with each DNA representing a whole genome in the set of DNA representing whole genomes to identify a match between that sequencing read and a DNA representing a whole genome;
determining, based on the comparing, a relative frequency of occurrence for each microorganism strain from the plurality of microorganism strains in the identified sample;
determining an absolute cell count of each microorganism strain from the plurality of microorganism strains in the identified sample based on the total quantity of microorganisms from the plurality of microorganism strains and the relative frequency of occurrence for each microorganism strain;
sequencing cDNA in the identified sample and mapping the sequenced cDNA to each DNA representing a whole genome in the set of whole genomes;
generating at least one of a transcriptional profile or a relative gene expression profile associated with each microorganism strain based on a quantity of cDNA reads mapped to the DNA representing a whole genome associated with that microorganism strain;
determining an activity for each microorganism strain in the identified sample based on the at least one of the transcriptional profile or the relative gene expression profile associated with that microorganism strain;
identifying active microorganism strains in the identified sample, based on the activity for each microorganism strain from the plurality of microorganism strains in the identified sample;
selecting at least one microorganism strain from active microorganism strains based on the transcriptional profile or the relative gene expression profile associated with each microorganism strain from the active microorganism strains; and
combining the selected microorganism strains with a carrier medium to form a bioensemble of active microorganisms configured to alter a property corresponding to at least one metadata of target biological environment when the bioensemble is introduced into that target biological environment.