Patent ID: 11859229
Assignee: HAYASHIBARA CO., LTD.
Field: Biotechnology (Chemistry)
Classification: CPC C  Y | IPC C

Claim 0:
1. A process for producing a polyvinyl alcohol-degrading enzyme, comprising the steps of:
culturing a microorganism capable of producing the polyvinyl alcohol-degrading enzyme in a nutrient medium;
removing cells from the resulting culture to obtain a supernatant; and
collecting the polyvinyl alcohol-degrading enzyme from the supernatant or collecting the supernatant as a crude enzyme preparation containing the polyvinyl alcohol-degrading enzyme;
wherein said polyvinyl alcohol-degrading enzyme comprises the following characteristics (1) to (3):
(1) comprising an activity of oxidizing polyvinyl alcohol and forming hydrogen peroxide;
(2) comprising an activity of hydrolyzing β-diketone; and
(3) exhibiting a molecular weight of 100,000±20,000 in SDS-polyacrylamide gel electrophoresis,

and wherein said polyvinyl alcohol-degrading enzyme comprises the amino acid sequence of SEQ ID NO: 2 or SEQ ID NO: 3, or an amino acid sequence comprising a deletion, a replacement, or an addition of one or more amino acid residues in the amino acid sequence of SEQ ID NO: 2 or SEQ ID NO: 3 while retaining the polyvinyl alcohol-degrading activity and comprising 84% or higher sequence identity to the amino acid sequence of SEQ ID NO: 2 or SEQ ID NO: 3.