Patent ID: 11884950
Assignee: OXFORD NANOPORE TECHNOLOGIES PLC
Field: Biotechnology (Chemistry)
Classification: CPC C  B | IPC B  C

Claim 10:
11. The method according to claim 3, wherein in step (1) and/or
(6): (a) the helper strand and the portion of the support strand hybridized thereto are connected by a hairpin loop; and
(b) the synthesis strand comprising the primer strand portion and the portion of the support strand hybridized thereto are connected by a hairpin loop;
optionally wherein at least one of the ligation polynucleotides is provided as a single molecule comprising a hairpin loop connecting the support strand and the helper strand at the end opposite the overhanging end; optionally wherein the ligation polynucleotides of each synthesis cycle are provided as single molecules each comprising a hairpin loop connecting the support strand and the helper strand at the end opposite the overhanging end.