Patent ID: 11939580
Assignee: RZNOMICS INC.
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 18:
19. A vector comprising a sequence encoding the self-circularization RNA construct of claim 1,
wherein the self-circularization RNA construct comprising, from the 5′-terminus to the 3′-terminus:
a) an internal guide sequence (IGS) region comprising guanine (G) at the 5′-end;
b) a ribozyme region wherein the ribozyme region is a Group I intron ribozyme;
c) a gene of interest (GOI) region; and
d) a fragment containing target splice site comprising uracil (U) at the 3′-end,

wherein the 5′-end G of the IGS region and the 3′-end U of the target splice site-containing fragment form a G : U wobble base pair, and
wherein the a) IGS region comprises a sequence of 2 to 10 contiguous nucleotides, which is reverse complementary to a 3′-end portion nucleotide sequence of the d) target splice site region.