Patent ID: 11971376
Assignee: EAST CHINA UNIVERSITY OF SCIENCE AND TECHNOLOGY
Field: Measurement (Instruments)
Classification: CPC G | IPC G

Claim 0:
1. A solid-state nuclear magnetic resonance (ssNMR) method for detecting a hydrogen bond structure, comprising the following steps:
(1) exciting a 1H nucleus of an RNA sample with a π/2 pulse;
(2) applying two π pulses every half rotation period on an X nucleus of the RNA sample;
(3) applying a n pulse on the 1H nucleus of the RNA sample;
(4) applying two n pulses every half rotation period on the X nucleus of the RNA sample;
(5) applying a 90° pulse on 1H and X atoms of the RNA sample;
(6) recording a chemical shift of X in indirect dimension;
(7) applying the 90° pulse on the 1H and X nuclei of the RNA sample;
(8) repeating steps 2, 3, and 4; and
(9) collecting the 1H signal in direct dimension; wherein
X is selected from the group consisting of 15N and 13C.