Patent ID: 11896978
Assignee: MESO SCALE TECHNOLOGIES, LLC.
Field: Chemical engineering (Chemistry)
Classification: CPC B  C | IPC B  C

Claim 0:
1. A method of conducting an analysis of a sample in an assay cartridge, said cartridge comprising a fluidic network and a plurality of chambers, wherein said fluidic network comprises:
a primary flow path and one or more fluidic conduits each intersecting said primary flow path and fluidically connecting said primary flow path to a chamber of said plurality of chambers, wherein said fluidic network is configured to meter a volume of fluid in said fluidic network, said method comprising the steps of:
(i) interfacing the fluidic network with a fluidic control manifold configured to drive fluid motion within said fluidic network;
(ii) metering a volume of sample from said chamber to a second chamber of the plurality of chambers;
(iii) metering a volume of lysis buffer from a third chamber of said plurality of chambers to said second chamber;
(iv) lysing said volume of sample;
(v) moving lysate from said second chamber to a purification zone of said primary flow path;
(vi) extracting nucleic acid from said lysate;
(vii) purifying said nucleic acid;
(viii) moving said purified nucleic acid to a PCR reaction zone of said primary flow path;
(ix) contacting said purified nucleic acid mixture with one or more PCR reagents to form a mixture;
(x) shuttling said mixture formed in step (ix) between first and second temperature controlled zones;
(xi) moving said mixture formed in step (x) to a detection zone; and
(xii) measuring a signal from said detection zone.