Patent ID: 11941861
Assignee: VENTANA MEDICAL SYSTEMS, INC.
Field: Measurement (Instruments)
Classification: CPC G | IPC G

Claim 19:
20. A non-transitory computer-readable medium storing instructions for removing contributions of autofluorescence in one or more channels of a multichannel image of a biological sample stained with one or more fluorescent labels, comprising:
independently calculating the contribution of autofluorescence in each channel of each pixel of the multichannel image based on: (i) at least two retrieved autofluorescence spectra; and (ii) normalized measured spectra for pixels of the multichannel image, and
subtracting from each measured pixel spectrum of the multichannel image the calculated contribution of autofluorescence in each channel for that respective pixel, wherein the at least two retrieved autofluorescence spectra are derived from measured signals corresponding to unlabeled portions of training multichannel images of a biological sample comprising one or more fluorescent labels.