Patent ID: 11967410
Assignee: MESO SCALE TECHNOLOGIES, LLC.
Field: Measurement (Instruments)
Classification: CPC G | IPC G

Claim 0:
1. A method of identifying injury severity in a human, wherein the injury is an absorbed dose of ionizing radiation or a radiation-induced tissue injury, the method comprising:
measuring levels of biomarkers in a panel of biomarkers in a sample from the human, wherein the sample is selected from the group consisting of whole blood, serum, plasma, and combinations thereof,
wherein the panel of biomarkers comprises salivary alpha-amylase, CD5, CD20, and Flt-3L; and
wherein the levels of the biomarkers in the panel of biomarkers are measured in a multiplexed immunoassay;
wherein the multiplexed immunoassay comprises:
(i) contacting the sample with (a) a plurality of capture antibodies for the biomarkers in the panel of biomarkers and (b) a plurality of detection antibodies for the biomarkers in the panel of biomarkers, wherein each detection antibody comprises an electrochemiluminescence (ECL) label; and
(ii) detecting the ECL label, thereby obtaining measured levels of the biomarkers in the panel of biomarkers;

fitting the measured levels of the biomarkers in the panel of biomarkers to a response surface model as a function of an injury severity index;
computing a cost function for combining the panel of biomarkers, wherein the cost function is, F
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wherein mi is a measured level of each biomarker, Mi is an expected level of each biomarker, and SI is the injury severity index; and
identifying an injury severity value that minimizes the cost function at a time interval between the injury and collection of the sample, thereby identifying the injury severity,
wherein the fitting, the computing, and the identifying are performed by a processor.