Patent ID: 11939630
Assignee: SHAANXI LIFEGEN CO., LTD.
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 5:
6. The method according to claim 4, wherein a basis for judging the final detection result is as follows:
specific primers and probes of a target gene and an internal reference gene are amplified in two separate reaction tubes on a fluorescent PCR instrument, and the fluorescence is collected through corresponding channels; the internal reference gene is used as the quality control, and a fluorescence amplification curve must be displayed, otherwise the result is regarded as invalid; on the premise of successful amplification of the internal reference gene:
if there is no amplification curve for the HLA-B*15:02 alleles positive reaction system while there is an amplification curve for the HLA-B*15:02 alleles negative reaction system, the sample is homozygous for HLA-B*15:02 allele negativity;
if there is a fluorescent signal corresponding to the forward primer for the HLA-B*15:02 positive allele, the sample is HLA-B*15:02 positive; on the basis of HLA-B*15:02 positivity, the sample is an HLA-B*15:02 positive heterozygote if there is an amplification curve for HLA-B*15:02 allele negativity, otherwise, the sample is an HLA-B*15:02 positive homozygote.