Patent ID: 11965187
Assignee: GUANGZHOU MEDICAL UNIVERSITY
Field: Pharmaceuticals (Chemistry)
Classification: CPC C  A | IPC A  C

Claim 0:
1. A fusion protein, comprising:
a truncated Campylobacter jejuni clustered regularly interspaced short palindromic repeat associated endonuclease 9 (CjCas9) and a truncated repressor of silencing 1 (ROS1) connected by an intermediate sequence comprising a linker;
wherein the truncated CjCas9 is an amino acid fragment obtained by removing amino acids at positions 481-640 of a CjCas9 protein, connecting remaining amino acids with a linker, and performing D8A point mutation; and
wherein the truncated ROS1 is an amino acid fragment obtained by removing amino acids at positions 1-509 and 628-855 of a ROS1 protein and connecting remaining amino acids with a linker, and an amino acid at position 971 of the ROS1 protein is aspartic acid;
wherein the fusion protein from an N-terminal to a C-terminal sequentially comprises:
the preamble sequence shown in SEQ ID NO: 24;
the sequence shown in SEQ ID NO:1, the linker shown in SEQ ID NO: 27, and the sequence shown in SEQ ID NO:2;
the intermediate sequence shown in SEQ ID NO: 23; and
the sequence shown in SEQ ID NO: 3, the linker shown in SEQ ID NO: 26, and the sequence shown in SEQ ID NO: 4.