Patent ID: 11867690
Assignee: ASEDASCIENCES AG
Field: Measurement (Instruments)
Classification: CPC G | IPC G

Claim 18:
19. A cell cytometry system, comprising, in one or more combined or separate parts:
(A) a cell cytometry apparatus for measuring the phenotypic characteristics of individual cells in a sample processed by a method comprising:
contacting aliquots of a population of cells with K different control conditions, where κ is at least 1, and with i different concentrations of an agent, where i is at least 1;
measuring p different phenotypic parameters, ψ, in individual cells of each aliquot, where p is at least 3 and, where ψp denotes a particular phenotypic parameter, thereby obtaining distributions Ck of the measured values for each control condition K for each phenotypic parameter ψp and distributions Si of the measured values for each concentration condition i for each phenotypic parameter ψp,, and
(B) a device for storing the measurements of the phenotypic characteristics of the individual cells and for analyzing the measurements by a gateless method that produces a multiparametric tensor fingerprint indicative of the response of the cells to the agent, said method comprising:
calculating pairwise distances d between the distributions of each control condition Ck and each concentration condition Si separately for each phenotypic parameter ψp, where

dκ,i(ψ)=D(Cκ(ψ),Ci(ψ)), and D is a distance function;, combining the calculated distances to form a multiparametric response tensor A, A
  =
  
   [
   
    
     
      
       a
       
        [
        
         
          κ
          1
         
         ,
         
          ψ
          1
         
        
        ]
       
      
     
     
      ⋯
     
     
      
       a
       
        [
        
         
          κ
          1
         
         ,
         
          ψ
          p
         
        
        ]
       
      
     
    
    
     
      ⋮
     
     
      ⋱
     
     
      ⋮
     
    
    
     
      
       a
       
        [
        
         
          κ
          m
         
         ,
         
          ψ
          1
         
        
        ]
       
      
     
     
      ⋯
     
     
      
       a
       
        [
        
         
          κ
          m
         
         ,
         
          ψ
          p
         
        
        ]
       
      
     
    
   
   ]
  
 

wherein each of a[κ, ψ] comprises all the pairwise distances calculated for one phenotypic parameter and one control:, a
   
    [
    
     κ
     ,
     ψ
    
    ]
   
  
  =
  
   [
   
    
     
      
       
        d
        
         κ
         ,
         1
        
        
         (
         ψ
         )
        
       
       =
       
        D
        ⁡
        (
        
         
          C
          κ
          
           (
           ψ
           )
          
         
         ,
         
          S
          1
          
           (
           ψ
           )
          
         
        
        )
       
      
     
    
    
     
      
       
        d
        
         κ
         ,
         1
        
        
         (
         ψ
         )
        
       
       =
       
        D
        ⁡
        (
        
         
          C
          κ
          
           (
           ψ
           )
          
         
         ,
         
          S
          2
          
           (
           ψ
           )
          
         
        
        )
       
      
     
    
    
     
      ⋮
     
    
    
     
      
       
        d
        
         κ
         ,
         1
        
        
         (
         ψ
         )
        
       
       =
       
        D
        ⁡
        (
        
         
          C
          κ
          
           (
           ψ
           )
          
         
         ,
         
          S
          n
          
           (
           ψ
           )
          
         
        
        )
       
      
     
    
   
   ], wherein the multiparametric response tensor A is a fingerprint characteristic of the effects of the agent on the cells that can be compared directly to other such fingerprints generated in the same way
wherein said fingerprints are free of gating bias, and
(C) a device for storing a databank comprising a plurality of the fingerprints,
wherein (A), (B) and (C) can be comprised in one or more than one instruments.