Patent ID: 11860101
Assignee: KOREA ADVANCED INSTITUTE OF SCIENCE AND TECHNOLOGY
Field: Measurement (Instruments)
Classification: CPC G | IPC G

Claim 0:
1. A multi-photon microscope exciting a fluorescent material in a sample using multi-photon excitation, the multi-photon microscope comprising:
a repetition rate tuner that lowers an optical pulse train emitted from a pulsed laser to a repetition rate for time-gated detection;
a scanner that scans the optical pulse train transmitted from the repetition rate tuner in x-axis and y-axis directions;
an objective lens that irradiates an optical signal scanned by the scanner to the sample and acquires a fluorescence signal emitted from the excited fluorescent material;
a photodetector that photoelectrically converts the fluorescence signal acquired by the objective lens;
an amplifier that converts a current signal output from the photodetector into a voltage signal;
a digitizer that samples the voltage signal output from the amplifier; and
a computing device that separates sampling data output from the digitizer with a detection window set in time domain, and generates an image using the sampling data separated by the detection window based on the repetition rate set by the repetition rate tuner, sampling information of the digitizer, and scanning information of the scanner.