Patent ID: 11939562
Assignee: PLURI BIOTECH LTD.
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 16:
17. A three-dimensional (3D) bioreactor for large scale expansion and activation of immune cells population the 3D bioreactor comprising:
at least one packed bed chamber;
at least one porous Antigen Presenting Cell Mimetic Scaffold (APC-MS) surrounded by said at least one packed bed chamber wherein said at least one porous APC-MS is coated with one or more extra cellular matrix protein (ECM);
at least one container surrounding said at least one packed bed chamber; and
a fluid media having at least one population of immune cells suspended in said fluid media contained in said at least one container;
wherein said fluid media having at least one population of immune cells suspended in said fluid media flows through said at least one porous APC-MS coated with one or more ECM; and
wherein, said at least one porous APC-MS coated with one or more ECM creates a stationary microenvironment having low shear forces that imitate the natural growth environment of said at least one population of immune cells; and
wherein said at least one porous APC-MS coated with one or more ECM has a porosity which allows large scale expansion and/or activation of said at least one population of immune cells that flow through it said at least one porous APC-MS coated with one or more ECM without immobilizing said at least one population of immune cells.