Patent ID: 11890397
Assignee: WENZHOU SAFETY (EMERGENCY) RESEARCH INSTITUTE, TIANJIN UNIVERSITY
Field: Medical technology (Instruments)
Classification: CPC A  C  Y | IPC A  C

Claim 0:
1. A anticoagulation extracorporeal circulation tube, comprising the following preparation methods:
S1, firstly, aminating a surface of a tube by extrusion molding, mixing PC or PVC plastic raw materials with raw materials rich in amino functional groups to form a mixture, adding the mixture into a hopper of an extrusion molding machine, and then entering a space of a screw groove by self-weight or under an action of a forced feeder, and moving to a head of the machine under a push of a screw flight, wherein all raw materials are conveyed forward by one lead every time a screw rotates; meanwhile, in an extrusion process, discharging a gas between the raw materials from the hopper by compressing, finally melting, cooling and molding, and cutting or cutting off as required, completing a grafting of raw materials rich in amino functional groups to an inner wall of the tube, and washing to obtain PVC or PC tube rich in amino groups;
S2, activating heparin groups by a direct coupling method, dissolving heparin sodium in citric acid buffer, adding 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide or 1-ethyl-3-(3-dimethyl aminopropyl) carbodiimide and N-hydroxysuccinimide, and stirring a solution in an ice-water bath for 1-5 h to activate a plurality of active groups of heparin to generate activated heparin containing carboxyl groups;
S3, heparinizing the PVC or PC tube, adding carboxylated heparin solution into the PVC or PC tube containing amino groups, refluxing for 1-20 hours, discharging the solution, repeating for 1-3 times, washing the PVC or PC tube in a mixed solution of ethanol and distilled water for 1-3 times, each time for 10-100 minutes, removing physically adsorbed heparin and other impurities, and then placing the PVC or PC tube in a vacuum drying oven and drying;
S4, after the heparinization of the PVC or PC tube is completed, using albumin, functionalized polyethylene glycol which can react with amino groups, and phosphate to further surface modify the inner wall of heparinized PVC or PC tube to block a plurality of sites not modified by heparinization, to shield the adsorption of platelets and protein in blood on the inner wall.