Patent ID: 11939585
Assignee: NANTBIO, INC.
Field: Pharmaceuticals (Chemistry)
Classification: CPC C  A | IPC A  C

Claim 14:
15. A method of transfecting mammalian cells with a RNA, comprising:
disposing the mammalian cells with the RNA in a transfection medium, wherein the mammalian cells are immune competent cells comprising NK cells, T-cells, B-cells, macrophages, or a combination thereof;
applying at least two pulses to the mammalian cells at a voltage of 150-250 V, a gap width of about 0.1 cm to about 0.4 cm, and a time constant from about 0.5 msec to about 10 msec to produce transfected mammalian cells, wherein the pulses are delivered from a capacitor having a capacitance of between 5-50 μF.