Patent ID: 11952605
Assignee: AMGEN INC.
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 0:
1. A method of modulating the amount of the mannose-5 glycoform of an Immunoglobulin G2 (IgG2) molecule in an IgG2 composition, wherein said IgG2 composition is produced by a Chinese Hamster Ovary (CHO) cell culture, comprising:
(a) establishing a cell culture of CHO cells in a bioreactor, wherein the CHO cell produces an IgG2 molecule;
(b) limiting the amount of glucose in the bioreactor, wherein the concentration of the glucose in the spent medium of the bioreactor is from about 0 to 3 g/L;
(c) supplementing the cell culture with a feeding medium comprising galactose, such that the concentration of galactose in the resulting spent medium of the bioreactor is above 2.5 g/L; and
(d) harvesting and purifying the IgG2 composition from the cell culture.