Patent ID: 11884940
Assignee: PROVIVI, INC.
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 0:
1. A method for increasing the selectivity of methyl palmitate conversion to a Z11-16 non-native unsaturated fatty acid over conversion to other lipid variants in a recombinant Yarrowia lipolytica microorganism, said method comprising the steps of:
a) oxidizing cytosolic malate to pyruvate in the recombinant Yarrowia lipolytica microorganism; wherein the oxidizing step is carried out by a heterologous decarboxylating malic enzyme from an organism selected from the group consisting of Escherichia coli, Hominidae sapiens, Lypomyces starkeyi, Mus musculus, Rattus novegicus, and Rhodosporidium toruloides; and
b) culturing said recombinant Yarrowia lipolytica microorganism in medium comprising a simple carbon substrate and methyl palmitate;
wherein the recombinant Yarrowia lipolytica microorganism exhibits increased selectivity in the conversion of methyl palmitate to the Z11-16 non-native unsaturated fatty acid compared to a control Yarrowia lipolytica microorganism in which the oxidizing step of (a) is not carried out.