Patent ID: 11873325
Assignee: LIMAGRAIN EUROPE
Field: Biotechnology (Chemistry)
Classification: CPC C  Y | IPC C

Claim 0:
1. A method for detecting the resistance allele of a plant resistant to Orange wheat blossom midge (OWBM) comprising the steps of:
a) isolating DNA genomic sequence or RNA nucleic acid from said plant,
b) subjecting said nucleic acid to successive rounds of amplification using fluorescently labeled primers to amplify a target sequence containing the allele and
c) detecting within the target sequence the presence of a resistance allele having the single nucleotide polymorphism selected from:
the T at position 32 in SEQ ID NO:9,
the G at position 85 in SEQ ID NO:11,
the T at position 50 in SEQ ID NO:13,
the C at position 58 in SEQ ID NO:15,
the A at position 88 in SEQ ID NO:17,
the C at position 90 in SEQ ID NO:19, and
the T at position 67 in SEQ ID NO:21.