Patent ID: 11912741
Assignee: GENZYME CORPORATION
Field: Chemical engineering (Chemistry)
Classification: CPC C  B | IPC C

Claim 0:
1. A continuous method for producing a recombinant protein, the method comprising:
(a) capturing the recombinant protein from a substantially cell-free sample using one or a plurality of capture chromatography systems and eluting the recombinant protein from the one or plurality of capture chromatography systems to produce eluates comprising the recombinant protein, wherein the eluates are homogenized into a single mixture comprising the recombinant protein;
(b) subjecting the homogenized single mixture to one or a plurality of post-capture chromatography systems and collecting product output comprising the recombinant protein; and
(c) subjecting the product output to ultrafiltration and diafiltration to purify the recombinant protein;
wherein each step is performed by a unit operation, wherein each unit operation is physically connected from step (a) to step (c), and wherein the connected unit operations from step (a) to step (c) process a continuous flow comprising the recombinant protein from step (a) to step (c).