Patent ID: 11933780
Assignee: FUNCTIONAL FLUIDICS INC.
Field: Measurement (Instruments)
Classification: CPC G  C | IPC C  G

Claim 0:
1. A method for assessing an effect on a cellular unit of an organism, comprising the steps of:
providing a first aliquot of a first sample including a first cellular unit not subject to the effect in a first supporting medium in a first hermetically sealed container;
providing a first aliquot of a second sample including a second cellular unit subject to the effect in a second supporting medium in a second hermetically sealed container;
deoxygenating the first supporting medium and the second supporting medium to induce at least one of a predetermined amount of hypoxia and a predetermined rate of oxygen consumption in the first supporting medium and in the second supporting medium;
determining, during deoxygenation of the first supporting medium, values for one or more variables associated with the first supporting medium at each of a first plurality of different times and/or values for one or more variables associated with the first cellular unit at each of the first plurality of different times;
determining, during deoxygenation of the second supporting medium, values for one or more variables associated with the second supporting medium at each of a second plurality of different times and/or values for one or more variables associated with the second cellular unit at each of the second plurality of different times;
correlating one of the predetermined amount of hypoxia and the predetermined rate of oxygen consumption in the first supporting medium with at least two of the other one of the predetermined amount of hypoxia and the predetermined rate of oxygen consumption in the first supporting medium, the values for the one or more variables associated with the first supporting medium, the values for the one or more variables associated with the first cellular unit and the first plurality of different times to generate a first multi-dimensional surface;
correlating one of the predetermined amount of hypoxia and the predetermined rate of oxygen consumption in the second supporting medium with at least two of the other one of the predetermined amount of hypoxia and the predetermined rate of oxygen consumption in the second supporting medium, the values for the one or more variables associated with the second supporting medium, the values for the one or more variables associated with the second cellular unit and the second plurality of different times to generate a second multi-dimensional surface;
identifying differences between the first multi-dimensional surface and the second multi-dimensional surface.