Patent ID: 11959110
Assignee: CLEVELAND STATE UNIVERSITY
Field: Pharmaceuticals (Chemistry)
Classification: CPC C  A | IPC A  C

Claim 3:
4. A method for producing an impotent prothrombin molecule having an amino acid sequence of prothrombin with an amino acid modification from residue 472 to 487, the method comprising:
(a) introducing into a host cell an expression vector which contains a nucleotide sequence which encodes a prothrombin molecule wherein the prothrombin molecule comprises a deletion of amino acid residues Ser478, Leu480, and Gln481 or a substitution of Ser478, Leu480, and Gln481 with alanine;
(b) culturing the host cell in an appropriate medium; and
(c) isolating the polypeptide product encoded by the expression vector.