Patent ID: 11946049
Assignee: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA
Field: Pharmaceuticals (Chemistry)
Classification: CPC A  C | IPC A  C

Claim 0:
1. A polynucleotide comprising a tRNA operably linked to a first pre-microRNA 34a (pre-miRNA-34a) and a second pre-miRNA-34a, wherein all or part of the stem-loop anticodon of the tRNA is replaced with the first pre-miRNA-34a and the second pre-miRNA-34a, wherein the first pre-miRNA-34a is operably linked to a first inserted RNA molecule, and the second pre-miRNA-34a is operably linked to a second inserted RNA molecule, wherein the first and second inserted RNA molecules are heterologous to the first pre-miRNA-34a and the second pre-miRNA-34a, wherein the first inserted RNA molecule is inserted at, abutted with, or operably linked to:
the 5′ end of the first pre-miRNA-34a;
the 3′ end of the first pre-miRNA-34a;
of a dicer or RNase cleavage site of the first pre-miRNA-34a; or
3′ of a dicer or RNase cleavage site of the first pre-miRNA-34a; and, wherein the second inserted RNA molecule is inserted at, abutted with, or operably linked to:
the 5′ end of the second pre-miRNA-34a;
the 3′ end of the second pre-miRNA-34a;
of a dicer or RNase cleavage site of the second pre-miRNA-34a; or
3′ of a dicer or RNase cleavage site of the second pre-miRNA-34a.