Patent ID: 11890339
Assignee: TRUSTEES OF DARTMOUTH COLLEGE
Field: Pharmaceuticals (Chemistry)
Classification: CPC A  C | IPC A  C

Claim 2:
3. The immunogen of claim 1, wherein the one or more amino acid substitutions comprise:
a) the cysteine substitutions at NiV F positions 104 and 114 that form a non-natural intra-protomer disulfide bond, and the proline substitution at NiV F position 191;
b) the cysteine substitutions at NiV F positions 104 and 114 that form a non-natural intra-protomer disulfide bond, and the phenylalanine substitution at NiV F position 172;
c) the cysteine substitutions at NiV F positions 104 and 114 that form a non-natural intra-protomer disulfide bond, the proline substitution at NiV F position 191, the phenylalanine substitution at NiV F position 172, and the glycine substitution at NiV F position 70;
d) the cysteine substitutions at NiV F positions 114 and 426 that form a non-natural intra-protomer disulfide bond, and the proline substitution at NiV F position 191;
e) the cysteine substitutions at NiV F positions 114 and 426 that form a non-natural intra-protomer disulfide bond, and the phenylalanine substitution at NiV F position 172;
f) the cysteine substitutions at NiV F positions 114 and 426 that form a non-natural intra-protomer disulfide bond, the proline substitution at NiV F position 191, and the phenylalanine substitution at NiV F position 172;
g) the cysteine substitutions at NiV F positions 114 and 426 that form a non-natural intra-protomer disulfide bond, the proline substitution at NiV F position 191, the phenylalanine substitution at NiV F position 172, and the glycine substitution at NiV F position 70;
h) the proline substitution at NiV F position 191, and the phenylalanine substitution at NiV F position 172;
i) the proline substitution at NiV F position 191, the phenylalanine substitution at NiV F position 172, and the glycine substitution at NiV F position 70;
j) the deletion of NiV F positions 102-113 with positions 101 and 114 linked by a glycine-serine linker, and the proline substitution at NiV F position 191,
k) the deletion of NiV F positions 102-113 with positions 101 and 114 linked by a glycine-serine linker, and the phenylalanine substitution at NiV F position 172; or
l) the deletion of NiV F positions 102-113 with positions 101 and 114 linked by a glycine-serine linker, the proline substitution at NiV F position 191, and the phenylalanine substitution at NiV F position 172.