Patent ID: 11898190
Assignee: KONAN GAKUEN
Field: Biotechnology (Chemistry)
Classification: CPC C  Y | IPC C

Claim 0:
1. A method for producing a microbial oil, comprising:
genetically modifying a labyrinthulid by disrupting and/or silencing a fatty acid desaturase gene;
culturing the labyrinthulid; and
collecting the microbial oil from the labyrinthulid,
wherein the labyrinthulid before the modification belongs to the genus Parietichytrium or the genus Schizochytrium having an activity of synthesizing DHA via a PUFA-PKS pathway in an amount of not more than 1/100 of a total amount of DHA synthesized in the labyrinthulid or no activity of producing PUFAs via the PUFA-PKS pathway, and having an activity of producing PUFAs via an endogenous elongase-desaturase pathway,
wherein the fatty acid desaturase gene is a Δ4 desaturase gene,
wherein the microbial oil satisfies (a) and (b):
(a) DHA is not greater than 0.50% of the total fatty acid composition; and
(b) a total of DHA and n-6 DPA is not greater than 0.7% of the total fatty acid composition.