Patent ID: 11860101
Assignee: KOREA ADVANCED INSTITUTE OF SCIENCE AND TECHNOLOGY
Field: Measurement (Instruments)
Classification: CPC G | IPC G

Claim 7:
8. An operation method of a multi-photon microscope exciting a fluorescent material in a sample using multi-photon excitation, the operation method comprising:
lowering an optical pulse train emitted from a pulsed laser to a repetition rate for time-gated detection;
scanning the optical pulse train lowered to the repetition rate in x-axis and y-axis directions;
irradiating the scanned optical pulse train to the sample;
generating sampling data by detecting a fluorescence signal emitted from the fluorescent material in the sample at specific time intervals;
separating a signal using a detection window set in time domain from the sampling data; and
generating an image using the sampling data separated by the detection window based on the repetition rate, sampling information used to generate the sampling data, and scanning information used to scan the optical pulse train.