Patent ID: 11857958
Assignee: UNIVERSITE GRENOBLE ALPES
Field: Biotechnology (Chemistry)
Classification: CPC B  C | IPC B  C

Claim 4:
5. A method for producing the microfluidic cell culture chip according to claim 4, wherein a production of the central module comprises:
a step of assembling a support part, consisting of a side frame, an open upper face and a solid lower face comprising a cut in a form of the central unit, and a mould, in shapes and dimensions of said support part, comprising at least one moulded 3D nanostructure on the upper face,
a step of pouring a resorbable polymer solution on said upper face of said mould, followed by
a step of polymerising said resorbable polymer solution to make rigid said resorbable polymer solution and form a resorbable polymer matrix comprising at least one negative mould of said at least one 3D nanostructure, followed by
a step of removing said mould, to obtain said resorbable polymer matrix comprising at least one negative mould of said at least one 3D nanostructure formed in said cut of the solid lower face of said support part, followed by
a step of assembling said support part, with a perforated part, comprising a support consisting of a non-resorbable membrane perforated by at least one perforation integrated in a base, said perforated part being in the shapes and dimensions of said support part, and the number of the at least one perforation of said perforated part being identical to the number of moulded 3D nanostructures in said mould used in the step of assembling said support part and said mould, such that said at least one perforation of said support, is aligned with said at least one negative mould of said at least one 3D nanostructure, followed by
a step of applying at least one continuous layer of at least one polyelectrolyte on the continuous surface consisting of the lower face of said support and the lower face of said resorbable polymer matrix comprising at least one negative mould of said at least one 3D nanostructure at the said at least one perforation of said support, to constitute the 3D nanostructured porous membrane comprising the at least one protuberance, followed by
a step of dissolving said resorbable polymer matrix comprising at least one negative mould of said at least one 3D nanostructure, and
a step of removing the support part to obtain said perforated part comprising on the lower face thereof, the 3D nanostructured porous membrane, and on the side of the upper face thereof, said at least one protuberance.