Patent ID: 11884703
Assignee: GENZYME CORPORATION
Field: Chemical engineering (Chemistry)
Classification: CPC C  B | IPC B  C

Claim 0:
1. A method of processing a fluid comprising a recombinant therapeutic protein, the method comprising:
(a) providing a circuit system comprising (i) a tangential flow virus filtration (TFVF) unit having first and second inlets, and (ii) a conduit in fluid communication between the first and second inlets of the TFVF unit, comprising at least one port for flowing fluid into or out of, or both, of the circuit system,
wherein the circuit system is configured such that fluid can be flowed through the conduit and the TFVF unit, and filtrate comprising the recombinant therapeutic protein can be collected from the TFVF unit;
(b) flowing a fluid including a recombinant therapeutic protein into the circuit system through one of the at least one port, recirculating retentate in the circuit system, and collecting filtrate comprising the recombinant therapeutic protein from the TFVF unit for a period of time;
wherein rate of recirculating the retentate in the circuit system and rate of flowing the fluid into the circuit system are independently controlled.