Patent ID: 11911611
Assignee: UNIVERSITY OF SOUTH FLORIDA
Field: Measurement (Instruments)
Classification: CPC A  G  C | IPC A  C

Claim 15:
16. A method for performing electroporation of one or more target cells, the method comprising:
introducing one or more molecules to be delivered to the one or more target cells;
contacting the one or more target cells with a multielectrode array (MEA);
connecting an impedance measurement system to the MEA;
measuring a first impedance of the one or more target cells using the MEA connected to the impedance measurement system;
disconnecting the impedance measurement system from the MEA following the measurement of the first impedance;
connecting an electric field generator instrument to the MEA after disconnecting the impedance measurement system from the MEA;
applying an electroporation protocol to the one or more target cells using the MEA connected to the electric field generator instrument, wherein the electroporation protocol comprises one or more adjustable parameters;
disconnecting the electric field generator instrument from the MEA following the application of the electroporation protocol;
reconnecting the impedance measurement system to the MEA after disconnecting the electric field generator instrument from the MEA;
measuring a second impedance of the one or more target cells using the impedance measurement system connected to the MEA following the application of the electroporation protocol;
comparing the first impedance and the second impedance of the one or more target cells; and
determining if the application of the first electroporation protocol has been effective in delivering the one or more molecules to the one or more target cells based upon the comparison of the first impedance and the second impedance of the one or more target cells, wherein determining if the application of the first electroporation protocol has been effective in delivering the one or more molecules to the one or more target cells based upon the comparison of the first impedance and the second impedance of the one or more target cells further comprises, determining if the second impedance is lower than the first impedance by a predetermined amount;
adjusting the one or more adjustable parameters of the electroporation protocol based upon the comparison of the first impedance and the second impedance of the one or more target cells; and
applying a subsequent electroporation protocol to the one or more target cells, wherein the subsequent electroporation protocol comprises the adjusted one or more adjustable parameters.