Patent ID: 11969480
Assignee: TRANSLATE BIO, INC.
Field: Pharmaceuticals (Chemistry)
Classification: CPC A  B  C | IPC A  B  C

Claim 0:
1. A process of encapsulating messenger RNA (mRNA) in preformed empty lipid nanoparticles (LNPs), wherein the lipid component of the 0.1:1NPs comprises or consists of a cationic lipid, a non-cationic lipid, a PEG-modified lipid, and optionally cholesterol and said process comprises the following steps:
adding a first volume of a solution comprising the mRNA to a suspension comprising the preformed empty LNPs such that a mixture comprising LNPs encapsulating the MRNA is formed;
adding one or more additional volumes of the solution comprising the mRNA to the mixture obtained in the preceding step until a desired molar ratio of cationic lipid to mRNA is achieved; and
wherein the first volume and the one or more additional volumes of the solution comprising the mRNA are added continuously without intervening periods of heating or mixing.