Patent ID: 11884952
Assignee: ZHEJIANG UNIVERSITY
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 3:
4. A production method of daptomycin using the Streptomyces griseus L340 according to claim 1, comprising the following steps:
(1) cutting spores from an agarslantculture-medium, inoculating the spores into a seed broth, and culturing on a shaker with a rotation speed of 250 rpm at 30° C. for 36 hours until a bacterial liquid is sticky and granular;
(2) harvesting 4% by volume of the bacterial liquid from the seed broth, inoculating into the fermentation medium, and culturing on a shaker with a rotation speed of 250 rpm at 30° C.; and after 48 hours, feeding decanoic acid in 0.1% by volume of the fermentation medium every 12 hours;
(3) after 144 hours, treating the fermentation broth with methanol of equal volume, and subjecting a supernatant obtained after centrifugation to subsequent separation and purification to obtain the daptomycin.