Patent ID: 11903967
Assignee: AUTOLUS LIMITED
Field: Pharmaceuticals (Chemistry)
Classification: CPC C  A | IPC A  C

Claim 0:
1. A method of preparing a population of genetically modified T cells which comprise a chimeric antigen receptor (CAR) or a transgenic T-cell receptor (TCR) comprising:
providing a starting population of T cells,
depleting said starting population of T cells which express a target antigen, and
introducing into the T cells in the depleted starting population a nucleic acid sequence which encodes a CAR or transgenic TCR against the target antigen,
wherein the target antigen is TCR beta constant region 1 (TRBC1) or TCR beta constant region 2 (TRBC2), and
wherein said genetically modified T cells are less exhausted compared with genetically modified T cells prepared without depleting said starting population of T cells which express the target antigen of the CAR or transgenic TCR.