Patent ID: 11859238
Assignee: GEN-PROBE INCORPORATED
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 0:
1. A method of amplifying a target nucleic acid sequence present in a sample, the method comprising the steps of:
(a) performing a first phase linear amplification reaction that amplifies the target nucleic acid sequence under conditions that do not support exponential amplification thereof,
wherein the first phase linear amplification reaction generates a first amplification product;

(b) performing a second phase amplification reaction under conditions allowing exponential amplification of the first amplification product, thereby generating a second amplification product that comprises the target nucleic acid sequence or the complement thereof; and
(c) detecting, with a sequence-specific hybridization probe at regular time intervals, synthesis of the second amplification product while the second phase amplification reaction is occurring.