Patent ID: 11884965
Assignee: LUMINEX CORPORATION
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 0:
1. A method for amplifying a target nucleic acid comprising:
(a) providing a first primer pair comprising:
(i) a first primer comprising:
a first target-specific primer sequence;
an anti-tag sequence 5′ of the target-specific primer sequence;
a tag sequence 5′ of the anti-tag sequence; and
a blocker between the anti-tag sequence and the tag sequence; and

(ii) a second primer comprising:
a second target-specific primer sequence; and

(b) providing a reporter;
(c) providing a capture complex comprising an anti-tag sequence attached to a solid support; and
(d) amplifying the target nucleic acid by combining the first primer pair, the reporter, the capture complex, and a sample comprising the target nucleic acid under conditions suitable for amplification of the target nucleic acid.