Patent ID: 11891651
Assignee: ELEMENT BIOSCIENCES, INC.
Field: Measurement (Instruments)
Classification: CPC C  G | IPC C  G

Claim 38:
39. The method of claim 1, wherein step (c) comprises
contacting the plurality of retained immobilized concatemer molecules with a second plurality of soluble forward sequencing primers, a plurality of nucleotides and a plurality of primer extension polymerases, under conditions suitable to hybridize the second plurality of soluble forward sequencing primers to the plurality of retained immobilized concatemer template molecules and conducting a polymerase-catalyzed primer extension reaction, thereby generating a plurality of forward extension strands replacing the plurality of extended forward sequencing primer strands,
wherein the plurality of nucleotides comprise dATP, dGTP, dCTP and dTTP but lacks dUTP,
wherein in the plurality of primer extension polymerases is tolerant of uridine-containing template strands, and
wherein the soluble sequencing primers hybridize with the forward sequencing primer binding sequence in the retained immobilized concatemer molecules.