Patent ID: 11860099
Assignee: ALDA S.R.L.
Field: Measurement (Instruments)
Classification: CPC G  C | IPC C  G

Claim 0:
1. An Antibody or a Fab fragment, wherein at least one amino group of the N-terminal amino acid of the light chain and/or of the N-terminal amino acid of the heavy chain is bound by an amidic bond to a molecule comprising a fluorophore group A, wherein said bond constitutes at least 70% of the total binding of said molecule to said antibody or said Fab;
wherein said amino group of the N-terminal amino acid of the light chain and/or of the N-terminal amino acid of the heavy chain is bound by an amidic bond to a Z group with formula —CO-A;
wherein said group A is selected from:
fluorophore CF568;
2-[(1E,3E)-5-[(2Z,3S)-3-(5-methoxy-5-oxopentyl)-3-methyl-5-sulfo-1-(3-sulfopropyl)-2,3-dihydro-1H-indol-2-ylidene]penta-1,3-dien-1-yl]-3,3-dimethyl-5-sulfo-1-(3-sulfopropyl)-3H-indol-2-yl (AF647), having structure, 2-(2,2,10,10-tetramethyl-4,8-bis(sulfonatomethyl)-2,10-dihydro-1H-pyrano[3,2-g:5,6-g′]diquinolin-11-ium-6-yl)terephthalate (AF568, both regioisomers), having one of the following structures, (7S,17R)-12-[4-(methoxycarbonyl)phenyl]-7,8,8,16,16,17-hexamethyl-2-oxa-6,18-diazapentacyclo[11.7.0.03,11.05,9.015,19]icosa-1(13),3,5,9,11,14,19-heptaen-6-ium-4,20-disulfonate (AF532), having structure, [10,10,22,22-tetramethyl-20-(sulfomethyl)-16-{2,3,4,5-tetrafluoro-6-[(4-methoxy-4-oxobutyl)(methyl)carbamoyl]phenyl}-3-oxa-9lambda4,23-diazaheptacyclo[17.7.1.15,9.02,17.04,15.023,27.013,28]octacosa-1,4,9(28),11,13,15,17,19(27),20-nonaen-12-yl]methanesulfonic acid (ASred), having structure, 2-({3-[12,20-bis(hydroxymethyl)-10,10,22,22-tetramethyl-3-oxa-9lambda4,23-diazaheptacyclo[17.7.1.15,9.02,17.04,15.023,27.013,28]octacosa-1,4,9(28),11,13,15,17,19(27),20-onaen-16-yl]-2,5,6-trifluoro-4-[(4-methoxy-4-oxobutyl)(methyl)carbamoyl]phenyl}sulfanyl)ethane-1-sulfonic acid (S635), having structure, ({10,10,22,22-tetramethyl-20-[(phosphonooxy)methyl]-16-{2,3,4,5-tetrafluoro-6-[(4-methoxy-4-oxobutyl)(methyl)carbamoyl]phenyl}-3-oxa-9lambda4,23-diazaheptacyclo[17.7.1.15,9.02,17.04,15.023,27.013,28]octacosa-1,4,9(28),11,13,15,17,19(27),20-nonaen-12-yl}methoxy)phosphonic acid, having structure,