Patent ID: 11926818
Assignee: IONTAS LIMITED
Field: Pharmaceuticals (Chemistry)
Classification: CPC C  A | IPC A  C  G

Claim 0:
1. A method of screening for a mammalian cell of a desired phenotype, wherein the phenotype results from expression of a binder by the mammalian cell, wherein the binder comprises or is linked to a membrane anchor, the method comprising:
providing donor DNA molecules encoding a diverse repertoire of binders, and mammalian cells,
introducing the donor DNA molecules into the mammalian cells and providing a site-specific nuclease within the cells, wherein the nuclease cleaves a recognition sequence in cellular DNA to create an integration site at which the donor DNA becomes integrated into the cellular DNA, integration occurring through DNA repair mechanisms endogenous to the mammalian cells, thereby creating recombinant cells containing donor DNA integrated at a fixed locus or at duplicate fixed loci in the cellular DNA, and
culturing the recombinant cells to produce clones, thereby providing a library of at least 105 mammalian cell clones containing the donor DNA encoding the repertoire of binders,
culturing the library cells to express the binders, wherein the binders are displayed on the surface of their expressing cells, and
detecting the desired phenotype using a cellular read-out.