Patent ID: 11898204
Assignee: ROCHE SEQUENCING SOLUTIONS, INC.
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 9:
10. A method of separately sequencing each strand of a target nucleic acid comprising the steps of:
a) in a reaction mixture, joining the ends of a double stranded target nucleic acid to adaptors to form a doubly-adapted target nucleic acid wherein the adaptor is a single strand forming a double stranded stem and a single-stranded loop, and the loop comprises a primer binding site and the adaptor further comprises strand synthesis termination site selected from an abasic nucleotide and a non-nucleotide linker and;
b) contacting the reaction mixture with an exonuclease thereby enriching for the doubly-adapted target nucleic acid;
c) contacting the reaction mixture with a primer capable of hybridizing to the primer binding sites;
d) extending the primers thereby separately sequencing each strand of the target nucleic acid, wherein extension is terminated at the strand synthesis termination site and does not proceed onto the complementary strand.