Patent ID: 11908548
Assignee: ILLUMINA, INC.
Field: Computer technology (Electrical engineering)
Classification: CPC G | IPC G

Claim 13:
14. A computer-implemented system including:
at least one processor;
a non-transitory computer readable memory including instructions that, when executed by at least a processor, cause the computer-implemented system to implement actions for determining metadata about clusters on a tile of a flow cell, the actions comprising:
accessing a set of images of the tile captured during a sequencing run and preliminary center coordinates of the clusters determined by a base caller;
for each image set, obtaining from the base caller, a base call classifying, as one of four bases, origin subpixels that contain the preliminary center coordinates and a predetermined neighborhood of contiguous subpixels that are successively contiguous to respective ones of the origin subpixels, thereby producing a base call sequence for each of the origin subpixels and for each of the predetermined neighborhood of contiguous subpixels;
generating a cluster map that identifies the clusters as disiointed regions of contiguous subpixels that are successively contiguous to at least some of the respective ones of the origin subpixels and share a substantially matching base call sequence of the one of four bases with the at least some of the respective ones of the origin subpixels; and
storing the cluster map in memory for use determining shapes and sizes of the clusters.