Patent ID: 11898142
Assignee: MASSACHUSETTS INSTITUTE OF TECHNOLOGY
Field: Measurement (Instruments)
Classification: CPC C  B  G  Y | IPC B  C  G

Claim 19:
20. The system of claim 1, wherein the one or more guide RNAs designed to bind to corresponding target nucleic acids comprise a (synthetic) mismatch; and
wherein said mismatch is optionally up- or downstream of a SNP or other single nucleotide variation in said target nucleic acid, optionally 1, 2, 3, 4, or 5 nucleotides upstream or downstream, optionally 2 nucleotides, optionally downstream of said SNP or other single nucleotide variation in said guide RNA; or
wherein the synthetic mismatch in said guide RNA is optionally at position 1, 2, 3, 4, 5, 6, 7, 8, or 9 of the spacer, optionally position 3 or position 5.