Patent ID: 11970723
Assignee: JIANGNAN UNIVERSITY
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 0:
1. A recombinant Bacillus subtilis (B. subtilis), obtained by transforming with an expression vector carrying a target gene D-allulose 3-epimerase into B. subtilis with an alanine racemase gene knocked out, the expression vector being obtained by:
replacing a P43 promoter on a pP43NMK plasmid carrying a D-allulose 3-epimerase gene with a hag promoter whose nucleotide sequence is set forth in SEQ ID NO. 2, and simultaneously mutating the base positions 129 to 137 on the sequence of the hag promoter to: ggggaggag; and replacing a resistance gene kanamycin Kan on the pP43NMK plasmid with an alanine racemase gene dal whose nucleotide sequence is set forth in SEQ ID NO. 19; or
replacing a P43 promoter on a pUB110 plasmid carrying a D-allulose 3-epimerase gene with a hag promoter whose nucleotide sequence is set forth in SEQ ID NO. 2, and simultaneously mutating the base positions 129 to 137 on the sequence of the hag promoter to: ggggaggag;
and replacing resistance genes kanamycin Kan and bleomycin Blm on the pUB110 plasmid with an alanine racemase gene dal whose nucleotide sequence is set forth in SEQ ID NO. 19.