Patent ID: 11959125
Assignee: SUN GENOMICS, INC.
Field: Pharmaceuticals (Chemistry)
Classification: CPC C  A  G | IPC A  C  G

Claim 0:
1. A method for preparing and analyzing a sample comprising:
a) extracting genetic material from a diverse population of microbes present in a first sample obtained from a subject using one or more of a detergent, a chelator, a glucoside hydrolase and a chaotropic agent and performing whole genome sequencing of DNA from the extracted genetic material,
wherein the diverse population of microbes comprises two or more microbes selected from the group consisting of bacteria, archaea, fungi, protozoa, helminths, parasites, viruses, phages, spores, and algae;
b) subjecting the extracted genetic material from the first sample to metagenomics analysis and quality filtering before taxonomy assignment;
c) measuring a diversity number for the first sample relative to an average diversity number and generating a microbiome score indicative of relative abundances of different types of microbes present in the first sample;
d) diagnosing the subject as having or at risk of having dysbiosis based on the metagenomics analysis of the first sample;
e) preparing and administering to the subject diagnosed as having or at risk of having dysbiosis, a customized probiotic, prebiotic, enzyme, vitamin supplement, mineral supplement, dietary supplement and/or botanical mixture which is formulated specifically to treat the dysbiosis in the subject and based on the metagenomics analysis and the microbiome score generated for the subject;
f) extracting genetic material from a diverse population of microbes present in a second sample obtained from the subject following the administering of e) in the same manner as the extraction of genetic material from the first sample in a);
g) performing metagenomics analysis on the extracted genetic material from the second sample and quality filtering before taxonomy assignment;
h) assessing the effectiveness of the dysbiosis treatment; and
i) administering to the subject a second customized probiotic, prebiotic, enzyme, vitamin supplement, mineral supplement, dietary supplement and/or botanical mixture which is formulated specifically to the dysbiosis in the subject and based on the assessment of effectiveness to treat the dysbiosis.