Patent ID: 11912773
Assignee: GENENTECH, INC.
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 0:
1. A prokaryotic host cell comprising a nucleic acid encoding a heteromultimeric protein or an IgG antibody, wherein the heteromultimeric protein or the IgG antibody each comprises an Fc region comprising substitution mutations at residues 241 and 243 on at least one heavy chain; wherein said substitution mutations are selected from the group consisting of (a) F241R and F243 S and (b) F241S and F243R, wherein numbering is according to the EU numbering scheme; wherein no amino acid residue of any Fc polypeptide of the heteromultimeric protein or the IgG antibody produced by the prokaryotic host cell is glycosylated; and wherein said heteromultimeric protein or IgG antibody exhibits decreased mispairing, decreased head-to-tail formation or increased overall yield as compared to a heteromultimeric protein or an IgG antibody without said amino acid substitution mutations at residues 241 and 243.