Patent ID: 11959100
Assignee: KYOTO UNIVERSITY
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 0:
1. A method for producing neural crest cells and maintaining their multipotency, comprising the steps of:
(1) obtaining neural crest cells by inducing the differentiation of stem cells into the neural crest cells; and
(2) suspension-culturing the neural crest cells in a medium comprising a GSK3β inhibitor and a basic fibroblast growth factor, wherein the medium comprises the GSK3β inhibitor with a concentration that exhibits an effect equivalent to that exhibited by CHIR99021 with a concentration of 3.0 μM to 4.5 μM, thereby maintaining multipotent neural crest cells over 63 days or longer, wherein the cultured neural crest cells comprise a ratio of at least 75% or more of neural crest cells that maintain multipotency to cultured cell population.