Patent ID: 11926847
Assignee: SANZYME BIOLOGICS PRIVATE LIMITED
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 0:
1. A swim-up medium for selective enrichment of fractions containing DNA-intact sperm cells post swim-up having a DNA fragmentation index (DFI) with DFIpost swim-up<DFIneat, from a semen sample, the swim-up medium comprising a solvent and a solute composition, said solute composition at a concentration of up to 15% w/v, and said solute composition further comprising:
a. a combination of electrolytes at a concentration range of 10% to 50% w/w consisting of sodium chloride, potassium chloride, magnesium sulphate, potassium di-hydrogen orthophosphate, calcium chloride, sodium bicarbonate, sodium phosphate monobasic anhydrous, magnesium chloride and glucose;
b. a combination of excipients at a concentration range of 1% to 30% w/w consisting of phenol red, HEPES buffer, gentamicin, sodium pyruvate, sodium lactate, glutamine, glycine, L-Arginine, and methionine;
c. Polyvinylpyrrolidone (PVP) at a concentration range of 3% to 80% w/w; and
d. recombinant Human Serum Albumin (rHSA) or bovine serum albumin (BSA) at a concentration range of 0.1% to 50% w/w;
wherein the said solvent is water.