Patent ID: 11965191
Assignee: CALIFORNIA INSTITUTE OF TECHNOLOGY
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 7:
8. The synthetic protein circuit of claim 6, wherein the fourth protease further comprises a first domain of the fourth protease, a second domain of the fourth protease, and an optional association domain of the fourth protease;
wherein the first domain of the fourth protease comprises the fourth protease first cut site specific for the second protease;
wherein the second domain of the fourth protease comprises a fourth protease second cut site specific for the second protease;
wherein the association domain of the fourth protease optionally comprises a first part of the fourth protease connected to the fourth protease first cut site and a second part of the fourth protease connected to the fourth protease second cut site; and
wherein the fourth protease is configured to be deactivated by cleavage of either of the fourth protease first cut site and the fourth protease second cut site.