Patent ID: 11965030
Assignee: SANOFI
Field: Biotechnology (Chemistry)
Classification: CPC A  C | IPC C

Claim 11:
12. The multispecific antigen-binding protein of claim 5, wherein at least one CH1/CL pair comprises CH1/CL mutations a CH1 region comprising K221E and K228D mutations at Kabat positions 221 and 228 and CL region comprising D122K and E123K mutations at Kabat positions 122 and 123 to facilitate pairing, and of
wherein at least one VH/VL pair comprises opposite charged mutations to facilitate pairing, said opposite charged mutations comprising (1) a mutated residue in the VH region at Kabat position 39 selected from E, D, K, R, or H, and (2) a mutated residue in the VL region at Kabat position 38 selected from E, D, K, R, or H, and
wherein the mutated residue in the VH region has an opposite charge from the mutated residue in the VL region.