Patent ID: 11920177
Assignee: SASYA INC.
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 14:
15. A method of producing βHIV using a non-natural microorganism, the method comprising:
culturing a non-natural microorganism in the presence of at least one carbon source to produce a fermentation-derived composition comprising βHIV, wherein the non-natural microorganism comprises a non-natural enzyme, wherein the non-natural enzyme has been modified or mutated to increase the ability of the enzyme to preferentially utilize α-ketoisocaproate as its substrate, and wherein the non-natural enzyme comprises one or more modifications or mutations at substrate-specificity positioins corresponding to amino acids from A361, F336, F347, F364, F368, F371, G362, I227, I252, L224, L289, L323, L367, N187, N241, N363, P239, Q251, Q265, S226, V212, V217, V228 and W210, of any of SEQ ID NO: 1 or SEQ ID NO: 6, and wherein the non-natural microorganism is modified or mutated to provide more βHIV than its wild-type parent; and isolating βHIV from the fermentation-derived composition.