Patent ID: 11913049
Assignee: RAMON GONZALEZ
Field: Biotechnology (Chemistry)
Classification: CPC C  Y | IPC C

Claim 5:
6. The method of claim 1, wherein said enzymes catalyzing the sequence of reactions for the regeneration of fumarate from the acyl-CoA or the keto-acid comprises:
a. an overexpressed propionyl-CoA carboxylase that catalyzes the carboxylation of propionyl-CoA to (S)-methyl-malonyl-CoA;
b. an overexpressed methyl-malonyl-CoA epimerase that catalyzes the interconversion of said (S)-methyl-malonyl-CoA to (R)-methyl-malonyl-CoA;
c. an overexpressed methyl-malonyl-CoA mutase that catalyzes the isomerization of said (R)-methyl-malonyl-CoA to succinyl-CoA;
d. an overexpressed succinyl-CoA:2-methyl-alkyl-succinyl-CoA transferase or succinyl-CoA synthetase that catalyzes the conversion of said succinyl-CoA to succinate; and
e. an overexpressed succinate dehydrogenase that catalyzes the conversion of said succinate to fumarate.