Patent ID: 11860099
Assignee: ALDA S.R.L.
Field: Measurement (Instruments)
Classification: CPC G  C | IPC C  G

Claim 9:
10. A method for the preparation of the antibody or the Fab according to claim 1, comprising the reaction between the antibody or the Fab fragment and a molecule Z—OB comprising a fluorophore group A and a —COOB group, and wherein the group B is a group having electrophile characteristics;
wherein said method comprises the following steps:
a) Preparation of a solution of said antibody or said Fab fragment in a buffer with a pH between 6 and 8;
b) Preparation of a solution of said molecule Z—OB, in a predetermined minimum necessary quantity of an anhydrous organic solvent;
c) Mixing of the two solutions prepared in a) and b), the two solutions are mixed, in the dark and at a temperature between 20° C. and 38° C., the two solutions are mixed in such a ratio that the ratio of equivalents of the antibody or the Fab and that of the molecule containing the fluorophore is between 0.5 and 2;
d) Addition to the solution resulting from step c) of an aqueous solution, a water-soluble primary amine, and a surfactant, in such quantity as to obtain a final concentration of primary amine of at least 0.3M, and incubation for at least 10 minutes of the mixture so obtained, then at a temperature of 4° C.