Patent ID: 11951155
Assignee: ZHONGSHI DUQING(SHANDONG) BIOTECH CO., LTD
Field: Pharmaceuticals (Chemistry)
Classification: CPC A | IPC A

Claim 3:
4. The method according to claim 1, wherein in step 6), the separation and purification by analytical liquid chromatography is specifically conducted under the following conditions: a chromatographic column at a predetermined flow rate using a mobile phase A comprising a trifluoroacetic acid aqueous solution %, and a mobile phase B comprising a trifluoroacetic acid acetonitrile solution; and a gradient elution process including the steps of:
eluting from 0 min to 5 min, with a volume percentage of the mobile phase A decreased from 95% to 90%, and a volume percentage of the mobile phase B increased from 5% to 10%;
eluting from 5 min to 55 min, with a volume percentage of the mobile phase A decreased from 90% to 15%, and a volume percentage of the mobile phase B increased from 10% to 85%; and
eluting from 55 min to 60 min, with a volume percentage of the mobile phase A increased from 15% to 95%, and a volume percentage of the mobile phase B decreased from 85% to 5%.