Patent ID: 11891651
Assignee: ELEMENT BIOSCIENCES, INC.
Field: Measurement (Instruments)
Classification: CPC C  G | IPC C  G

Claim 25:
26. The method of claim 1, wherein the reverse sequencing of step (e) comprises:
a) contacting a plurality of sequencing polymerases with (i) the plurality of retained forward extension strands and (ii) a plurality of the soluble reverse sequencing primers, wherein the contacting is conducted under conditions suitable to form a plurality of complexed polymerases comprising a sequencing polymerase bound to a nucleic acid duplex wherein the nucleic acid duplex comprises a retained forward extension strand hybridized to a soluble reverse sequencing primer;
b) contacting the plurality of complexed polymerases with a plurality of nucleotides under conditions suitable for binding at least one nucleotide to the complexed polymerases, wherein the plurality of nucleotides comprises at least one nucleotide analog labeled with a fluorophore and having a removable chain terminating moiety at the sugar 3′ position;
c) incorporating at least one nucleotide into the 3′ ends of the hybridized reverse sequencing primers thereby generating a plurality of nascent extended reverse sequencing primers; and
d) detecting the incorporated nucleotide and identifying the nucleo-base of the incorporated nucleotide.