Patent ID: 11912980
Assignee: DSM IP ASSETS B.V.
Field: Biotechnology (Chemistry)
Classification: CPC C  A | IPC A  C

Claim 2:
3. A method for the production of a desired bacterial strain comprising a genetic trait of interest, comprising
a) providing a composition comprising:
i) a precursor of the desired bacterial strain, wherein the precursor lacks the genetic trait of interest; and
ii) a donor bacterial strain comprising the genetic trait of interest;
wherein at least one bacterial strain in the composition comprises an increased amount of expression product of a pilin gene cluster compared to that of Lactococcus lactis subsp. cremoris NCDO 712 when cultured under identical conditions;

b) incubating the composition under conditions conducive to conjugation, and
c) optionally isolating the desired bacterial strain comprising the desired genetic trait of interest.