Patent ID: 11946098
Assignee: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA
Field: Measurement (Instruments)
Classification: CPC C  G  A | IPC A  C  G

Claim 2:
3. The method of claim 1, wherein
the binding of the target to the recognition element may occur in three or more binding states; and
the concentration of the target species, [T], is calculated by an equation, [
   T
   ]
  
  =
  
   
    K
    DM
   
   ⁢
   
    
     i
     -
     
      α
      ⁢
      
       i
       M
      
     
    
    
     
      
       γα
       M
      
      ⁢
      
       i
       M
      
     
     -
     i
    
   
  
 

wherein i is the measured output value at the responsive pulse frequency;
wherein iM is the measured output value at the minimally responsive pulse frequency;
wherein γ is a constant comprising the ratio of target-saturated output signal to target-free output signal at the responsive frequency for sensors of the selected design operated under the selected assay conditions;
wherein KDM is utilized as a dissociation constant and represents a mid-point of an iliM versus target concentration curve for sensors of the selected design operated under the selected assay conditions; and
wherein αM is a constant comprising the ratio of iM output signal to target-free output signal at the responsive frequency for sensors of the selected design operated under the selected assay conditions.