Patent ID: 11970718
Assignee: CARMINE THERAPEUTICS PTE. LTD.
Field: Pharmaceuticals (Chemistry)
Classification: CPC C  A  B | IPC A  B  C

Claim 15:
16. A method of loading a population of RBCEVs with a DNA cargo,
which method comprises steps of:
contacting the population of RBCEVs with a cationic reagent and the DNA cargo for a period of time sufficient for the DNA cargo to be loaded into RBCEVs of the population so that a loaded preparation is prepared,

wherein the RBCEVs have a size between 50 nm and 1,000 nm in diameter;
the DNA cargo has a length between 2,000 bases and 40,000 bases;
the cationic reagent complexes with the DNA cargo and is a polyethylenimine (PEI) reagent; and further wherein the loaded preparation is characterized in that,
when contacted with recipient mammalian cells, it is more effective at transfecting the mammalian cells with the DNA cargo than is an otherwise comparable reference preparation loaded by electroporation.