Patent ID: 11953436
Assignee: PROTEINSIMPLE
Field: Measurement (Instruments)
Classification: CPC G | IPC A  B  C  G

Claim 3:
4. The method of claim 1, wherein:
at least some protein isoforms from the plurality of protein isoforms have one or more disulfide groups;
the one or more free thiol groups is a first set of free thiol groups; and
the conjugated thiols are a first set of conjugated thiols, the method further comprising:
generating, from the sample, a third sample portion and a fourth sample portion;
applying, to the third sample portion and the fourth sample portion, a reducing agent configured to reduce disulfide groups to generate a second set of one or more free thiol groups;
applying, to the third sample portion, the fluorescent detection binder such that the fluorescent detection binder binds to the first set of free thiol groups and the second set of free thiol groups to produce a second set of conjugated thiols;
separating the plurality of protein isoforms in the third sample portion to generate a third set of separated protein isoforms;
separating the plurality of protein isoforms in the fourth sample portion to generate a fourth set of separated protein isoforms;
detecting a third fluorescence signal associated with the second set of conjugated thiols and a second absorbance signal associated with the third set of separated protein isoforms;
identifying, based on the second absorbance signal, at least one of a quantity or an identity of each separated protein isoform from the third set of separated protein isoforms;
detecting a fourth fluorescence signal associated with the fourth set of separated protein isoforms; and
measuring, based on a difference between the third fluorescence signal and the fourth fluorescence signal, a quantity of the first set of free thiol groups and a quantity of disulfide groups associated with each separated protein isoform from the third set of separated protein isoforms.