Patent ID: 11890617
Assignee: MIROCULUS INC.
Field: Chemical engineering (Chemistry)
Classification: CPC B | IPC B  G

Claim 0:
1. A method of replenishing a reaction droplet within an air gap region of a microfluidic apparatus to correct for evaporation, the method comprising:
monitoring a reaction droplet in the air gap of the microfluidic apparatus for a change in an optical intensity;
determining, based on the change in the optical intensity, when a volume of the reaction droplet falls below a threshold, wherein the reaction droplet comprises a solvent and reaction reagents;
introducing a replenishing droplet into the air gap of the microfluidic apparatus, wherein the replenishing droplet consists of solvent; and
combining the replenishing droplet with the reaction droplet after the volume of the reaction droplet falls beneath the threshold.