Patent ID: 11898205
Assignee: 10X GENOMICS, INC.
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 0:
1. A method for preparing a spatial array, the method comprising:
(a) contacting a substrate comprising a plurality of capture probes with a padlock probe, wherein:
(i) a capture probe of the plurality of capture probes comprises a first docking sequence, a spatial barcode, a capture domain, and a second docking sequence; and
(ii) the padlock probe comprises:
a first docking padlock sequence at its 3′ end that is complementary to the first docking sequence of the capture probe, and
a second docking padlock sequence at its 5′ end that is complementary to the second docking sequence of the capture probe;

(b) hybridizing the first docking sequence to the first docking padlock sequence and hybridizing the second docking sequence to the second docking padlock sequence;
(c) ligating the padlock probe, thereby generating a ligated padlock probe that comprises the capture domain or a complement thereof; and
(d) amplifying the ligated padlock probe, thereby generating an amplified padlock probe sequence with multiple copies of the capture domain.