Patent ID: 11957102
Assignee: YEDA RESEARCH AND DEVELOPMENT CO. LTD.
Field: Biotechnology (Chemistry)
Classification: CPC A  C | IPC A  C

Claim 25:
26. A method of producing at least one phytosterol in a modified plant, the method comprising
(a) transforming at least one plant cell with at least one silencing molecule targeted to a nucleic acid sequence encoding a GAME15 cellulose synthase like factor; or
(b) mutagenizing at least one gene or a combination of genes, the genes encoding the GAME15 cellulose synthase like factors, wherein the mutagenesis comprises introduction of one or more point mutations into the gene, or genome editing, or use of a bacterial CRISPR/CAS system, or a combination thereof,
wherein expression of the gene encoding the cellulose synthase like protein is reduced in the modified plant compared to its expression in a corresponding unmodified plant, thereby the modified plant comprises reduced content at least one steroidal alkaloid or a glycosylated derivative thereof, or of at least one steroidal saponin or a glycosylated derivative thereof, compared to the corresponding unmodified plant; and wherein the modified plant further comprises an elevated content of a phytosterol or a derivative thereof, a cholesterol or a derivative thereof, a phytocholesterol or a derivative thereof, a cholestenol or a derivative thereof, a phytocholestanol or a derivative thereof, or a steroidal saponin or a derivative thereof compared to a corresponding unmodified plant.