Patent ID: 11920175
Assignee: ZHEJIANG MEDICINE CO., LTD. XINCHANG PHARMACEUTICAL FACTORY
Field: Organic fine chemistry (Chemistry)
Classification: CPC C | IPC C

Claim 0:
1. A method for extracting and isolating lutein crystal from vegetable oil resin containing lutein diester, comprising the following steps:
a) dissolving a lipase Est076 (C23) into deionized water to form an enzyme solution;
b) dissolving a lutein extract into an alcohol solvent containing deionized water at a temperature of 30-65° C. to form a uniform alcohol solution, wherein the alcohol solvent is ethanol, isopropanol, n-propanol, or propylene glycol, and wherein the deionized water in the alcohol solvent is 5-15% (w/w);
c) adding the enzyme solution to the alcohol solution for performing a hydrolysis reaction at a temperature of 30-65° C. under stirring, until the lutein fatty acid ester is completely hydrolyzed to lutein, to obtain a lutein solution;
d) immediately filtering the lutein solution at a temperature of 30-65° C., and separating by pressure filtration to obtain a lutein crystal filter cake;
e) re-dissolving the lutein crystal filter cake into a non-polar organic solvent at a temperature of 20-110° C., and washing using deionized water to remove a water-soluble impurity;
f) partly recycling the non-polar organic solvent and cooling same to obtain a recrystallized crystals; and
g) isolating the recrystallized crystals and then vacuum drying or freeze drying, to obtain lutein crystals, wherein a final dry weight loss of the lutein crystals is less than 5%.