Patent ID: 11953440
Assignee: INL—INTERNATIONAL IBERIAN NANOTECHNOLOGY LABORATORY
Field: Measurement (Instruments)
Classification: CPC G | IPC G

Claim 4:
5. A system configured to perform the method described in claim 1 for the simultaneous multicolour nonlinear imaging of multiple chromophores of different types in a sample, comprising:
a) an excitation light system emitting laser pulses from a few-cycle laser system for the excitation of the multiple chromophores;
b) a focusing element adjusted to produce a near diffraction-limited laser spot of said excitation laser pulses a sample plane;
c) a pulse characterization and compression section configured to control the focused laser pulses to deliver near-transform-limited pulses at the sample plane;
d) an optical element guiding the laser pulses to the sample plane and consequently direct the nonlinear signal to the detectors;
e) an optical element preventing the laser pulses from directly reaching the detectors;
f) a photon counting electronics configured to detect single photons and photon arrival times from the generated non-linear signal from the sample;
g) a scanning section configured to perform excitation and measurement on individual points (1D) or point-by-point in the sample plane (2D) or point by point across focal planes (3D);
h) a control section for correlation of the detection with the scanning in order to acquire nonlinear signal associated to the scanning position(s) at the sample plane;
i) a signal processing section configured to provide information related with the time resolved nonlinear signal;
j) an analysis section software configured to determine the decay parameters associated to the photon arrival time histograms in each of individual points (1D) or point-by-point in the sample plane (2D) or point by point across focal planes (3D).