Patent ID: 11912599
Assignee: PHAREM BIOTECH AB
Field: Environmental technology (Chemistry)
Classification: CPC C  Y | IPC C

Claim 12:
13. A method of purifying sewage water from a plurality of environmentally hazardous organic substances, comprising the steps of:
a) providing one or more polynucleotide(s) encoding a plurality of enzymes, chosen from a group consisting of epoxide hydrolase, P450 BM3, melamine deaminase, acetoacetate decarboxylase and LinB, and wherein the plurality of enzymes are modified to eliminate the plurality of environmentally hazardous organic substances at a pH in a range of 5.5 to 8.5 and a temperature in a range of +8° C. to 20° C. having the capacity to eliminate the plurality of the environmentally hazardous organic substances, chosen from drug molecules, while maintaining an activity against the environmentally hazardous organic substances of at least 103 s−1M−1 (kcat/Km);
b) producing the plurality of enzymes recombinantly in an organism transformed with a vector, comprising the one or more polynucleotides from a), wherein the plurality of enzymes is produced by said organism, thereby obtaining an enzyme solution;
c) adding the enzyme solution to a filter, wherein the filter comprises a material having the capacity to bind the plurality of enzymes, wherein the material of the filter is a H2O stable open-cell foam material, wherein the plurality of enzymes bind(s) covalently to said material, wherein the filter is to be put in contact with the sewage water to be purified from the plurality of environmentally hazardous organic substances;
d) using the filter in a sewage water treatment system wherein the sewage water is to be purified from the plurality of environmentally hazardous organic substances, such that the filter enzymatically the plurality of environmentally hazardous organic substances from the sewage water to an extent of 60-95%.