Patent ID: 11866793
Assignee: REGENTS OF THE UNIVERSITY OF MINNESOTA
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 0:
1. A method for determining whether a sample contains Amaranthus palmeri plant material, the method comprising:
a. providing a primer set comprising:
i. a first primer that recognizes a first locus in the genome of Amaranthus and is specific to the sequence variant found in Amaranthus palmeri, 
ii. a second primer that recognizes the first locus in the genome of Amaranthus and is specific to the sequence variant found in all Amaranthus species other than Amaranthus palmeri, and
iii. a third primer that recognizes a second locus in the genome of Amaranthus and is specific to a sequence found in all Amaranthus species,
wherein the first primer comprises SEQ ID NO: 1 and the second primer comprises SEQ ID NO: 2 or the first primer comprises SEQ ID NO: 4 and the second primer comprises SEQ ID NO: 5;

b. obtaining a sample comprising DNA derived from at least one Amaranthus plant;
c. forming a reaction mixture by contacting the primer set with the sample under conditions in which the first primer and second primer each form a primer pair with the third primer, such that the primer pairs bind to and amplify nucleotide sequences in the sample that are recognized by the primer pairs;
d. amplifying DNA in the sample, wherein any DNA amplified using the first primer forms a first amplification product and any DNA amplified using the second primer forms a second amplification product;
e. detecting whether the first amplification product is present following amplification, wherein the presence of the first amplification product indicates that the sample contains Amaranthus palmeri plant material.