Patent ID: 11905311
Assignee: ESTAR TECHNOLOGIES LTD.
Field: Chemical engineering (Chemistry)
Classification: CPC C  B  A | IPC A  B  C

Claim 0:
1. A method for producing of autologous IL-1 receptor antagonist (IL1-RA) cytokine in a closed vessel tube system, the method comprising the steps of:
a. obtaining a vessel tube system for producing autologous IL-1RA cytokine comprising:
(i) an incubation vessel comprising washed and pretreated borosilicate glass beads in a size of about 0.5-5 mm for collection of a blood sample; and
(ii) a vessel cover;

b. filling said incubation vessel with whole blood sample;
c. incubating said incubation vessel with said whole blood sample for 30 minutes to 24 hours at 25-37° C.;
d. centrifuging said vessel for about 10-30 minutes; and, filtering an enriched plasma fraction obtained from said vessel after centrifuging with a filter having a pore size of 0.2-50 μm such that a concentration of said autologous IL-1RA cytokine relative to said whole blood sample is increased.