Patent ID: 11859196
Assignee: NATIONAL RESEARCH COUNCIL OF CANADA
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 3:
4. A method of obtaining a genetically engineered Brassicaceae plant having increased drought tolerance, the method comprising:
(i) transforming at least one Brassicaceae plant cell with an expression cassette encoding at least one inhibitory polynucleotide that targets an endogenous Kanghan gene in the transformed Brassicaceae plant cell to reduce or eliminate expression of a Kanghan protein encoded by the endogenous Kanghan gene;
(ii) obtaining at least one genetically engineered Brassicaceae plant from said at least one transformed Brassicaceae plant cell produced in step (i); and
(iii) selecting a genetically engineered Brassicaceae plant from said at least one genetically engineered Brassicaceae plant obtained in step (ii) that exhibits increased drought tolerance relative to a control Brassicaceae plant or plant cell of the same species and grown under the same conditions, into which the inhibitory polynucleotide has not been introduced, wherein the inhibitory polynucleotide is expressed by the at least one transformed Brassicaceae plant cell produced in step (i), wherein the inhibitory polynucleotide is a CRISPR guide RNA, and wherein the Kanghan protein has at least 95% amino acid sequence identity to the amino acid sequence as set forth in SEQ ID NO: 85.