Patent ID: 11952430
Assignee: REGENERON PHARMACEUTICALS, INC.
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 0:
1. A multispecific antigen-binding molecule, comprising:
(a) a first polypeptide comprising, from N-terminus to C-terminus (i) a first antigen-binding domain that specifically binds human CD3, wherein the first antigen-binding domain comprises an immunoglobulin Fab domain comprising a heavy chain variable region (HCVR) and a heavy chain CH1 domain paired with a light chain variable region (LCVR) and a light chain CL domain; (ii) a first multimerizing domain comprising an immunoglobulin Fc domain; and (iii) a second antigen-binding domain that specifically binds human CD3, wherein the second antigen-binding domain is a single chain variable fragment (scFv) domain comprising a HCVR and a LCVR connected by a peptide linker; and
(b) a second polypeptide comprising, from N-terminus to C-terminus (i) a third antigen-binding domain that specifically binds a target antigen, wherein the third antigen-binding domain comprises an immunoglobulin Fab domain comprising a HCVR and a heavy chain CH1 domain paired with a LCVR and a light chain CL domain; (ii) a second multimerizing domain comprising an immunoglobulin Fc domain; and (iii) a fourth antigen-binding domain that specifically binds a target antigen, wherein the fourth antigen-binding domain is a scFv domain comprising a HCVR and a LCVR connected by a peptide linker,
wherein the first and the second multimerizing domains associate with one another to form the molecule.