Patent ID: 11963978
Assignee: MEDICAL CORPORATION ICHIKAWA CLINIC
Field: Pharmaceuticals (Chemistry)
Classification: CPC A  C | IPC A  C

Claim 0:
1. A method for producing a composition having cytocidal activity, the method comprising:
culturing malignant tumor cells obtained from a malignant tumor or from an established malignant tumor cell line in a culture medium to a 60 to 100% confluent state;
replacing, after the culturing, the culture medium with a physiological buffer salt solution; and
recovering the physiological buffer salt solution after the time at which death of the malignant tumor cells is observed in the physiological buffer salt solution in morphological aspect of said cells,
obtaining a composition comprising a fraction with a molecular weight of 1 kDa or less from the recovered physiological buffer salt solution, wherein the composition comprises a component produced by the malignant tumor cells and causing themselves to die.