Patent ID: 11891656
Assignee: SINGULAR GENOMICS SYSTEMS, INC.
Field: Measurement (Instruments)
Classification: CPC C  G | IPC C  G

Claim 26:
27. A method of detecting a protein and a nucleic acid molecule in a cell, said method comprising:
i) contacting the cell with a specific binding reagent and binding the specific binding reagent to the protein, wherein the specific binding reagent comprises an oligonucleotide;
ii) contacting the cell with a first polynucleotide probe comprising a first barcode sequence, and binding the first polynucleotide probe to the nucleic acid molecule;
iii) contacting the cell with a second polynucleotide probe comprising a second barcode sequence, and binding the second polynucleotide probe to the oligonucleotide;
iv) amplifying the first polynucleotide probe thereby forming a first amplification product comprising the first barcode sequence, or complement thereof, and amplifying the second polynucleotide probe thereby forming a second amplification product comprising the second barcode sequence, or complement thereof;
v) hybridizing a first sequencing primer to the first amplification product and sequencing the first barcode sequence, or a complement thereof; followed by hybridizing a second sequencing primer to the second amplification product and sequencing the second barcode sequence.