Patent ID: 11959109
Assignee: SEIKAGAKU CORPORATION
Field: nan (nan)
Classification: CPC nan | IPC C  G

Claim 0:
1. A method for producing an endotoxin assay agent comprising a horseshoe crab Factor C, a horseshoe crab Factor B, and a horseshoe crab Pro-clotting enzyme, the method comprising:
expressing the horseshoe crab Factor C in a Chinese Hamster Ovary cell or a Human Embryonic Kidney cell as a host cell, secreting the horseshoe crab Factor C into an extracellular culture supernatant, and recovering the horseshoe crab Factor C in the culture supernatant, wherein the recovered horseshoe Factor C has activity of Factor C, and wherein the horseshoe crab Factor C is a Tachypleus tridentatus Factor C or a Limulus polyphemus Factor C,
expressing the horseshoe crab Factor B having activity of Factor B in a mammalian cell as a host cell,
expressing the horseshoe crab Pro-clotting enzyme in a mammalian cell as a host cell, and
producing an endotoxin assay agent comprising the horseshoe crab Factor C, the horseshoe crab Factor B, and the horseshoe crab Pro-clotting enzyme,
wherein the host cell for expressing the horseshoe crab Factor C, the host cell for expressing the horseshoe crab Factor B, and the host cell for expressing the horseshoe crab Pro-clotting enzyme may be the same or different.