Patent ID: 11857958
Assignee: UNIVERSITE GRENOBLE ALPES
Field: Biotechnology (Chemistry)
Classification: CPC B  C | IPC B  C

Claim 2:
3. A method for producing the microfluidic cell culture chip according to claim 2, wherein a production of said central unit comprises:
a step of extruding a resorbable polymer solution, through the at least one perforation of said support to form at least one 3D nanostructure on the side of said upper face of said support, followed by
a step of polymerising said resorbable polymer solution to make rigid said at least one 3D nanostructure, said at least one 3D nanostructure forming a resorbable polymer mould on the side of said upper face of said support after polymerisation, followed by
a step of applying at least one continuous layer of at least one polyelectrolyte covering the upper face of said support and a surface of said resorbable polymer mould, to constitute the 3D nanostructure porous membrane, followed by
a step of dissolving said resorbable polymer mould to obtain said lower face of said 3D nanostructured porous membrane positioned and secured to said upper face of said support, an entirety of said inner face of the at least one protuberance facing said at least one perforation.