Patent ID: 11913014
Assignee: INTEGRATED DNA TECHNOLOGIES, INC.
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 0:
1. An isolated nucleic acid encoding a mutant Cas9 protein selected from the group consisting of
(a) a single substitution mutation introduced into the WT-Cas9 protein of SEQ ID NO:5 selected from the following positions: N690, F682, K684 and D686; or
(b) a double substitution mutation introduced into the WT-Cas9 protein of SEQ ID NO:5 selected from two of following positions: N690, F682, L683, K684 and D686; or
(c) a double substitution mutation introduced into the WT-Cas9 protein of SEQ ID NO:5 comprising any one of following positions: N690, F682, L683, K684 and D686 in combination with any one of the following positions: R494, N522, N588, N612, T657, S663, R691, N692, S730, T740, R765, T770, N776, R778, R783, S793, N803, S845, N854, S872 and R925.