Patent ID: 11879158
Assignee: GUARDANT HEALTH, INC.
Field: Biotechnology (Chemistry)
Classification: CPC C  G | IPC C  G

Claim 0:
1. A method, comprising:
(a) providing at least one set of tagged parent polynucleotides by converting initial starting genetic material, which is cell-free nucleic acid, into the tagged parent polynucleotides, wherein converting comprises tagging by enzymatic ligation with n different molecular barcodes, wherein n is at least 2 and no more than 10,000*z, wherein z is a mean of an expected number of duplicate molecules in the plurality of polynucleotides having identical start and stop positions;
(b) amplifying the tagged parent polynucleotides in the set to produce a corresponding set of amplified progeny polynucleotides;
(c) sequencing at least a subset of the set of amplified progeny polynucleotides to produce set of sequencing reads;
(d) collapsing the set of sequencing reads to generate a set of consensus sequences, wherein a consensus sequence corresponds to a unique polynucleotide among the set of tagged parent polynucleotides; and
(e) analyzing the set of consensus sequences using one or more bioinformatics processes to detect methylation profiles, wherein the analyzing comprises determining a quantitative measure of polymorphic forms from among a plurality of consensus sequences, wherein the polymorphic forms comprise methylation, hypermethylation, and hydroxymethylation.