Patent ID: 11959849
Assignee: BECTON, DICKINSON AND COMPANY
Field: Measurement (Instruments)
Classification: CPC G | IPC G

Claim 20:
21. A method of assembling a flow cytometer, the method comprising:
(a) positioning a light collection enhancer within a flow cytometer, wherein:
the flow cytometer comprises:
a flow cell for transporting particles in a flow stream;
a light source for irradiating the particles in the flow stream at an interrogation point; and

an objective lens for focusing particle-modulated light propagating within a first light collection cone; and
a light collection enhancer configured to collect particle-modulated light propagating along an optical path within a second light collection cone and redirect the collected light such that it is back-propagated along the same optical path and focused by the objective lens; and
(b) optically aligning the light collection enhancer with the objective lens to assemble the flow cytometer.