Patent ID: 11964997
Assignee: REALSEQ BIOSCIENCES, INC.
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 12:
13. The method of claim 1, comprising converting the 5′ end or 3′ end, and comprising contacting the plurality of adapter-polynucleotide ligation products with an enzyme selected from:
a) a polynucleotide kinase in the presence or in absence of ATP;
b) a modified polynucleotide kinase derivative lacking 3′-end phosphatase activity in the presence of ATP; or in the absence of ATP and optional presence of ADP
c) an alkaline phosphatase;
d) an RNA 5′ monophosphatase;
e) RNA 5′ polyphosphatase; and
f) a pyrophosphatase.