Patent ID: 11959139
Assignee: GUARDANT HEALTH, INC.
Field: Biotechnology (Chemistry)
Classification: CPC C  G | IPC C  G

Claim 0:
1. A method for treating a subject having melanoma, comprising:
(a) providing a sample comprising double-stranded deoxyribonucleic acid (DNA) molecules from the subject;
(b) attaching adapters comprising molecular barcodes to a plurality of the double-strand DNA molecules to produce tagged parent polynucleotides;
(c) amplifying a plurality of the tagged parent polynucleotides to produce amplified progeny polynucleotides;
(d) sequencing a plurality of the amplified progeny polynucleotides to produce a set of sequencing reads;
(e) mapping a plurality of the sequencing reads from the set of sequencing reads to a reference sequence to generate mapped sequencing reads;
(f) reducing or tracking redundancy in a plurality of the mapped sequencing reads using at least sequence information from the molecular barcodes, wherein the reducing or tracking redundancy comprises generating a plurality of consensus sequences representative of original DNA molecules from among the tagged parent polynucleotides, wherein the plurality of consensus sequences is generated from (i) paired reads corresponding to mapped sequencing reads generated from a first tagged strand and a second tagged complementary strand derived from a DNA molecule from among the tagged parent polynucleotides, and (ii) unpaired reads corresponding to sequence reads generated from a first tagged strand having no second tagged complementary strand derived from a DNA molecule from among the tagged parent polynucleotides;
(g) detecting a genetic variant associated with a BRAF V600E mutation from among the plurality of consensus sequences; and
(h) administering a therapy comprising dabrafenib to the subject to treat the melanoma based on detecting the presence of the genetic variant associated with the BRAF V600E mutation.