Patent ID: 11927738
Assignee: PATHWARE INC.
Field: Measurement (Instruments)
Classification: CPC G | IPC G

Claim 25:
26. A near-field Fourier ptychographic system for assessing a biological sample, comprising: at least one light source configured to illuminate a biological sample positioned relative to the at least one light source;
a diffuser configured to diffuse light produced by the at least one light source, wherein the light is diffused in a speckled pattern either before the light interacts with the biological sample or after the light has interacted with the biological sample;
a sensor configured to capture a plurality of illuminated images with an embedded speckle pattern based on the diffused light; and
a processor and a non-transitory computer-readable medium with instructions stored thereon, wherein the processor is arranged to execute the instructions, the processor being further arranged to:
characterize a speckled pattern to be applied by the diffuser,
iteratively reconstruct the plurality of speckled illuminated images of the biological sample to recover an image stack of reconstructed images,
stitch together each image in the image stack to create a whole slide image, wherein each image of the image stack at least partially overlaps with a neighboring image, and
identify one or more features of the biological sample, wherein the one or more features are selected from a group consisting of: cell count, nucleus, edges, groupings, clump size, and a combination thereof.