Patent ID: 11884951
Assignee: AJINOMOTO CO., INC.
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 0:
1. A method for producing objective RNA, the method comprising:
A) culturing a coryneform bacterium having an expression unit for the objective RNA in a medium, to express the objective RNA and accumulate the objective RNA in cells of the bacterium; and
B) collecting the objective RNA from the cells,
wherein the bacterium has been modified so that the activity of ribonuclease III is reduced as compared with a non-modified strain by attenuating the expression of a gene encoding ribonuclease III;
wherein the ribonuclease III is a protein selected from the group consisting of:
(a) a protein comprising the amino acid sequence of SEQ ID NO: 52;
(b) a protein comprising the amino acid sequence of SEQ ID NO: 52, but which includes substitution, deletion, insertion, and/or addition of 1 to 10 amino acid residues, and having ribonuclease III activity; and
(c) a protein comprising an amino acid sequence having an identity of 90% or higher to the amino acid sequence of SEQ ID NO: 52, and having ribonuclease III activity.