Patent ID: 11904010
Assignee: BOEHRINGER INGELHEIM VETMEDICA (CHINA) CO., LTD.
Field: Pharmaceuticals (Chemistry)
Classification: CPC A  C | IPC A  C

Claim 17:
18. A recombinant virus, comprising an expression cassette in the genome of the recombinant virus, wherein the expression cassette comprises a first polynucleotide encoding at least one polypeptide of interest, and a second polynucleotide which is an essential gene of the recombinant virus or a functional fragment thereof;
wherein the first polynucleotide is functionally linked to the second polynucleotide with the first polynucleotide being located upstream of the second polynucleotide;
wherein said second polynucleotide is the only copy of said essential gene or functional fragment there that is active in the recombinant virus;
wherein the first polynucleotide and the second polynucleotide are within the same ORF;
wherein the expression cassette further comprises regulatory elements; and
wherein the expression cassette comprises a P1-2A-3C-2A-ORF43 construct or a P1-2A-3C-2A-ORF54 construct.