Patent ID: 11898203
Assignee: THE GENERAL HOSPITAL CORPORATION
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 8:
9. The method of claim 8, wherein the linear dsDNA oligonucleotides comprise potential DNA substrate sequences comprising:
(i) a set of all potential off-target sequences for the adenine base editing enzyme in a reference genome bearing up to a certain number of substitutions, single base pair deletions, and/or single base pair insertions relative to an identified on-target site for the adenine base editing enzyme;
(ii) a comprehensive set of all potential off-target sequences for the adenine base editing enzyme bearing up to a certain number of substitutions, single base pair deletions, and/or single base pair insertions relative to an identified on-target site for the adenine base editing enzyme;
(iii) a set of potential off-target sequences bearing up to a certain number of substitutions, single base pair deletions, and/or single base pair insertions relative to an identified on-target site for the adenine base editing enzyme;
(iv) a set of all potential off-target sequences for the adenine base editing enzyme in the coding sequence of a human reference genome bearing up to a certain number of substitutions, single base pair deletions, and/or single base pair insertions relative to an identified on-target site for the adenine base editing enzyme; or
(v) a set of all potential off-target sequences for the adenine base editing enzyme in the sequence of an oncogene hotspot and/or tumor suppressor gene of a reference genome bearing up to a certain number of substitutions, single base pair deletions, and/or single base pair insertions relative to an identified on-target site for the adenine base editing enzyme.