Patent ID: 11965192
Assignee: CODEXIS, INC.
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 0:
1. A method for producing phenyl acetate mono-protected or di-protected insulin, comprising: i) providing an engineered penicillin G acylase having a polypeptide sequence that is at least 95% identical to SEQ ID NO: 1220, wherein said penicillin G acylase comprises a leucine residue at position X2 as compared to the sequence of SEQ ID NO: 1220 and free insulin; and ii) exposing said engineered penicillin G acylase to said insulin, under conditions such that said engineered penicillin G acylase acylates up to two positions selected from the group of positions consisting of the A1 position of insulin, the B1 position of insulin, and the B29 position of insulin, thereby producing mono-protected or di-protected insulin.