Patent ID: 11971419
Assignee: ENZO LIFE SCIENCES, INC.
Field: Measurement (Instruments)
Classification: CPC G  C | IPC C  G

Claim 0:
1. A method of assaying for 24(S)-hydroxycholesterol in a fluid or tissue sample from a mammal, the method comprising
combining the sample with antibodies that specifically bind to 24(S)-hydroxycholesterol,
wherein the antibodies are generated by (a) preparing a 24(S)-hydroxycholesterol immunogen by conjugating 3-O-succinoyl-24(S)-hydroxycholesterol to a carrier protein for immunization of a rabbit and the carrier protein is attached through a linkage at the 3-position; (b) immunizing the rabbit with the 24(S)-hydroxycholesterol immunogen under conditions such that the immune system of the rabbit makes antibodies to 24(S)-hydroxycholesterol; and (c) collecting the anti-24(S)-hydroxycholesterol polyclonal antibodies from the rabbit; and
detecting whether the antibodies specifically bind to 24(S)-hydroxycholesterol from the sample;, wherein specific antibody binding to 24(S)-hydroxycholesterol from the sample indicates that 24(S)-hydroxycholesterol is present in the sample.