Patent ID: 11873485
Assignee: CALIFORNIA INSTITUTE OF TECHNOLOGY
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 0:
1. An allosteric conditional guide RNA (cgRNA) comprising a 5′ fragment (cg5) and a 3′ fragment (cg3), cg5 comprising a target-binding region and a trigger-binding region, and cg3 comprising a cognate RNA trigger:
a. wherein the trigger-binding region is non-overlapping with the target-binding region and is not configured to bind either the target-binding region or 5′ of the target-binding region;
b. wherein cg5 and cg3 are inactive when not bound to each other; and
c. wherein upon hybridization of cg3 to cg5 to form a cg5:cg3 complex comprising a terminator duplex, the cgRNA is activated, mediating the function of a Cas protein effector on a target gene that binds the target-binding region,
wherein:
fragment cg5 comprises a trigger-binding region comprising a 5′ portion of a stem of the terminator duplex; and
fragment cg3 comprises a 3′ portion of the stem of the terminator duplex, such that hybridization of cg5 to cg3 forms the terminator duplex, activating the cgRNA.