Patent ID: 11932859
Assignee: REGENERON PHARMACEUTICALS, INC.
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 22:
23. The method of claim 22, wherein the cleaving by the first nuclease agent and/or the second nuclease agent removes a double-stranded fragment from an end of the first nucleic acid at which the seamless assembly will occur, and
wherein the first joiner oligo further comprises a spacer between the first complementary sequence and the second complementary sequence, or the third joiner oligo further comprises a spacer between the first complementary sequence and the second complementary sequence, and
wherein the spacer comprises a sequence identical to the fragment, wherein no nucleic acid bases are present between the first complementary sequence and the sequence identical to the fragment, and no nucleic acid bases are present between the second complementary sequence and the sequence identical to the fragment.