Patent ID: 11864554
Assignee: THE CURATORS OF THE UNIVERSITY OF MISSOURI
Field: Basic materials chemistry (Chemistry)
Classification: CPC A | IPC A

Claim 13:
14. A method for preserving tendon tissue at room temperature in a chamber comprising serum-free culture medium comprising dexamethasone prior to implantation, the method comprising: placing the tendon tissue in a chamber base, the chamber base with said serum-free culture medium, the chamber base configured to maintain the tissue and said serum-free culture medium;
forming a tissue preservation chamber by covering the chamber base with a lid to form a barrier to contaminants, the chamber comprising at least one filter, a media inlet coupled to at least one filter for maintaining a sterile environment inside the chamber, and a media outlet, the media outlet including a media outlet conduit that extends into the chamber to permit removal of medium; wherein the media outlet comprises a one-way valve to prevent reentry of medium exiting the chamber; and storing the tendon tissue at room temperature for from about 7 days to about 70 days prior to implantation, wherein at least 70% of the cells of said tendon tissue remain viable after said storing compared to the viability of the cells of the tendon tissue at day 0.