Patent ID: 11919006
Assignee: INDIAN INSTITUTE OF TECHNOLOGY, KHARAGPUR
Field: Measurement (Instruments)
Classification: CPC B  G  C | IPC B  C  G

Claim 12:
13. The method as claimed in claim 1, comprising using primer sequences, for SARS-CoV-2 detection, selected from the group consisting of:, Target

Primer

Region
Primer
Sequence (5′ to 3′)
Modification

ORF 1b
F3
GCCATTAGTGCAAAGAATAGAGC

SEQ ID

NO: 1

B3
GGCATGGCTCTATCACATTTAGG

SEQ ID

NO: 2

FIP
TAGCTCCTCTAGTGGCGGCTATTGCACCGT
5′-[Btn]

(F1c + F2)
AGCTGGTGTCTC

SEQ ID

NO: 3

BIP
TGTAGTAATTGGAACAAGCAAATTCTATGG

(B1c + B2)
TGGCCAACCCATAAGGTGAGGG

SEQ ID

NO: 4

Loop F
TTTTTGATGAAACTGTCTATTGGTCATAGT

SEQ ID
ACTACAG

NO: 5

Loop B
GGCACAACATGTTAAAAACTGTTTATAGTG

SEQ ID
ATGTAG

NO: 6

BLP
TTGGCACAACATGTTAAAAACTGTTTATAG
5′-[6 

Probe
TGATG
fluorescein

SEQ ID

amidite]

NO: 7

BLP 3′-
TTGGCACAACATGTTAAAAACTGTTTATAG
5′-[6

mod Probe
TGATG
fluorescein

SEQ ID

amidite],

NO: 8

3′-[3d_G]

FLP Probe
GCGGCTATTGATTTCAATAATTTTTGATGA
5′-[6

SEQ ID
AAC
fluorescein

NO: 9

amidite]

 

N gene
F3
ACAATGTAACACAAGCTTTCG

SEQ ID

NO: 10

B3
TTGGATCTTTGTCATCCAATT

SEQ ID

NO: 11

FIP
GGCCAATGTTTGTAATCAGTTCCTTAGACG
5′-[Btn]

(F1c + F2)
TGGTCCAGAACAA

SEQ ID

NO: 12

BIP
GCTTCAGCGTTCTTCGGAATCACCTGTGTA

(B1c + B2)
GGTCAACC

SEQ ID

NO: 13

Loop F
TGGTCCCCAAAATTTCCTTGG

SEQ ID

NO: 14

Loop B
CGCGCATTGGCATGGAAGT

SEQ ID

NO: 15

BLP
TTGGCATGGAAGTCACACCTTC
5′-[6

Probe

fluorescein

SEQ ID

amidite-]

NO: 16

BLP 3′-
TTGGCATGGAAGTCACACCTTC
5′-[6

mod Probe

fluorescein

SEQ ID

amidite],

NO: 17

3′-[3d_C]

FLP Probe
GATTAGTTCCTGGTCCCCAAAATTTCC
5′-[6

SEQ ID

fluorescein

NO: 18

amidite]

 

E gene
F3
TTGTAAGCACAAGCTGATG

SEQ ID

NO: 19

B3
AGAGTAAACGTAAAAAGAAGGTT

SEQ ID

NO: 20

FIP
CGAAAGCAAGAAAAAGAAGTACGCTAGTAC
5′-[Btn]

(F1c + F2)
GAACTTATGTACTCATTCG

SEQ ID

NO: 21

BIP
GGTATTCTTGCTAGTTACACTAGCCAAGAC

(B1c + B2)
TCACGTTAACAATATTGC

SEQ ID

NO: 22

Loop F
ATTAACGTACCTGTCTCTTCCGAAA

SEQ ID

NO: 23

Loop B
ATCCTTACTGCGCTTCGATTGTGTG

SEQ ID

NO: 24

BLP
ATCCTTACTGCGCTTCGATTGTGTG
5′-[6

Probe

fluorescein

SEQ ID

amidite-]

NO: 25

BLP 3′-
ATCCTTACTGCGCTTCGATTGTGTG
5′-[6

mod Probe

fluorescein

SEQ ID

amidite],

NO: 26

3′-[3d_G]

FLP Probe
ATTAACTATTAACGTACCTGTCTCTTCC
5′-[6

SEQ ID

fluorescein

NO: 27

amidite-]

 

RNaseP
F3
TTGATGAGCTGGAGCCA

SEQ ID

NO: 28

B3
CACCCTCAATGCAGAGTC

SEQ ID

NO: 29

FIP
GTGTGACCCTGAAGACTCGGTTTTAGCCAC
5′-[Btn]

(F1c + F2)
TGACTCGGATC

SEQ ID

NO: 30

BIP
CCTCCGTGATATGGCTCTTCGTTTTTTTCT

(B1c + B2)
TACATGGCTCTGGTC

SEQ ID

NO: 31

Loop F
ATGTGGATGGCTGAGTTGTT

SEQ ID

NO: 32

Loop B
CATGCTGAGTACTGGACCTC

SEQ ID

NO: 33

BLP Probe
CATGCTGAGTACTGGACCTCG
5′-[6

SEQ ID

fluorescein

NO: 34

amidite]

FLP Probe
ATGTGGATGGCTGAGTTGTT
5′-[6

SEQ ID

fluorescein

NO: 35

amidite].