Patent ID: 11892448
Assignee: HOFFMANN-LA ROCHE, INC.
Field: Measurement (Instruments)
Classification: CPC G | IPC G

Claim 4:
5. The method according to claim 1, comprising:
a) determining the free drug fraction in at least three samples with different drug:target ratios,
wherein in the at least three samples the amount of drug is kept constant and the amount of target is different in each sample, with the proviso that the sample comprises excess target compared to drug,
or
determining the free target fraction in at least three samples with different drug:target ratios,
whereby in the at least three samples the amount of target is kept constant and the amount of drug is different in each sample, with the proviso that the sample comprises excess drug compared to target,
b) calculating based on the fraction of free drug or calculating based on the fraction of free target determined in the previous step the binding affinity of the drug to its target for each sample,
wherein the binding affinity has been determined if the at least three KD values are comparable,
wherein the method is repeated if the at least three KD values are not comparable using samples in which either
i) the amount of target is reduced at constant amount of the drug, or
ii) the amount if drug is reduced at constant amount of the target, or compared to the samples used in the previous determination.