Patent ID: 11946855
Assignee: BIO-RAD LABORATORIES, INC.
Field: Measurement (Instruments)
Classification: CPC G  B  C | IPC B  C  G

Claim 0:
1. A method comprising:
providing a substrate comprising:
a sample inlet,
an outlet channel, and
a set of chambers in fluid communication with the sample inlet and the outlet channel, wherein flow from the sample inlet is configured to reach the outlet channel only by passing into the set of chambers;
receiving a sample comprising a set of cells into the sample inlet, thereby isolating single cells of the set of cells within individual chambers of the set of chambers;
receiving a set of molecule-coated microspheres into the sample inlet,

thereby capturing the set of molecule-coated microspheres with the set of cells at h set of chambers;
flowing an isolation layer over the set of chambers to seal the set of chambers; and
processing the set of cells with a cell lysing reagent at the set of chambers; and
performing a single cell assay with cell lysate of the set of cells.