Patent ID: 11970728
Assignee: HENAN AGRICULTURAL UNIVERSITY
Field: Measurement (Instruments)
Classification: CPC C  G  A | IPC A  C

Claim 0:
1. A method for identifying resistance of wheat to black point disease caused by Bipolaris sorokiniana in an indoor environment, comprising the steps of:
a) a step of surface sterilization of wheat seeds, comprising:
selecting full wheat seeds and immersing the wheat seeds in alcohol, and rinsing the wheat seeds with sterilized distilled water following the immersion to generate sterilized wheat seeds;
b) a step of cultivating wheat seedlings from sterilized wheat seeds, comprising:
placing sterilized wheat seeds from step a) in Petri dishes, placing said Petri dishes in an incubator, and then cultivating said sterilized wheat seeds for 10 days;
c) a step of preparing a conidial suspension of Bipolaris sorokiniana, comprising:
cutting Bipolaris sorokiniana that have been stored in a refrigerator at 4° C. into 0.3 cm2 pieces and inoculating in a potato dextrose agar medium, culturing at 25° C. in the dark to generate a cultured colony, scraping conidia from the cultured colony with a glass slide, rinsing the potato dextrose agar mediums with distilled water to obtain a fungal suspension, and filtering the fungal suspension with gauze to remove mycelial fragments and obtain a conidia suspension;
d) a step of inoculating the conidia suspension on leaves of a wheat seedling and identifying diseased leaves, comprising:
shaking the conidia suspension of Bipolaris sorokiniana obtained in step c) and spraying the conidia suspension on leaves of a wheat seedling which has grown to a one-leaf-one-shoot stage in step b), covering the wheat seedling with a transparent plastic cover, and placing the wheat seedling in an incubator and culturing for 10 days at 25° C.;
e) a step of recording identification results, comprising:
recording a percentage of diseased leaf area within a total leaf area of a leaf of the wheat seedling cultivated in step d) on the 10th day of inoculation to obtain a percentage of diseased leaf area;
f) a step of calculating black point incidence in wheat, comprising:
calculating a percentage of black point grains from the percentage of diseased leaf area by using the equation y=−1.0037x+66.1360; wherein in the equation, y and x are the black point incidence and the percentage of diseased leaf area, respectively, comprising;
g) a step of evaluating resistance to black point disease, comprising:
evaluating resistance of wheat to black point disease caused by Bipolaris sorokiniana based on the black point incidence calculated in step f);
wherein in step c), after incubating Bipolaris sorokiniana at 25° C. in dark for 7 days, observing the state of conidia production, wherein a gray color of colonies indicates a relatively lower yield of conidia production and a darker color indicates a relatively higher yield of conidia, and continuing the culture process while removing Petri dishes when the colonies become the darker color.