Patent ID: 11937951
Assignee: TRUSTEES OF DARTMOUTH COLLEGE
Field: Pharmaceuticals (Chemistry)
Classification: CPC A | IPC A

Claim 12:
13. A method of identifying tumor tissue of a subject during surgery comprising:
administering a first fluorescent contrast agent to the subject, the first fluorescent contrast agent selected from the group consisting of fluorescent contrast agents targeted to the tumor tissue and containing a targeted fluorophore, and agents containing a prodrug metabolizable by the tumor tissue into the targeted fluorophore;
administering a second fluorescent contrast agent to the subject, the second fluorescent contrast agent having tissue affinity differing from a tissue affinity of the first fluorescent contrast agent and containing a second fluorophore;
illuminating tissue of the subject with light of a first stimulus wavelength;
acquiring first data at a first emissions wavelength associated with the targeted fluorophore;
acquiring second data at a second emissions wavelength associated with the second fluorophore, the second emissions wavelength different from the first emissions wavelength; and
subtracting the second data from the first data to provide corrected fluorescence data;
reconstructing bound fluorophore concentrations of a three-dimensional voxel-based model of tissue of the subject using the corrected fluorescence data; and
wherein the targeted fluorophore emits light of the first emissions wavelength when stimulated by light of the first stimulus wavelength, and the second fluorophore emits light of the second emissions wavelength when stimulated by light of the second stimulus wavelength.