Patent ID: 11926853
Assignee: THE RESEARCH FOUNDATION FOR MICROBIAL DISEASES OF OSAKA UNIVERSITY
Field: Biotechnology (Chemistry)
Classification: CPC C  Y | IPC C

Claim 0:
1. A method for producing a botulinum toxin, comprising steps of:
(A) producing a botulinum toxin from a botulinum toxin-producing bacterium in a culture medium to produce a mixture a containing a bacterial component and a nucleic acid component both derived from the botulinum toxin and the botulinum toxin;
(B) subjecting the mixture a to a removal of the bacterial component to produce a mixture b containing the nucleic acid component and the botulinum toxin;
(C) adding an endonuclease to the mixture b to produce a mixture c containing a nucleic acid degradation product and the botulinum toxin; and
(D) subjecting the mixture c to a removal of the nucleic acid degradation product to produce an isolated botulinum toxin liquid d,
wherein the method excludes any acid precipitation step.