Patent ID: 11920129
Assignee: NATIONAL UNIVERSITY OF SINGAPORE
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 10:
11. The method according to claim 2, further comprising the steps of:
(iv) using the single nucleic acid molecule from step (iii) as a template for amplifying in a polymerase chain reaction with an amplification primer having a sequence designed based on at least part or all of the first defined sequence and comprising at least one modified nucleotide and another amplification primer having a sequence designed based on at least part or all of the second defined sequence and comprising at least one modified nucleotide to produce an amplicon, chemically cleaving the amplicon to produce a first end with a third overhang and a second end with a fourth overhang; and
(v) performing a ligation to join the amplicon to a plurality of nucleic acid molecules to form an assembly of joined plurality of nucleic acid molecules; wherein each of the plurality of nucleic acid molecules is an amplicon from step (iv).