Patent ID: 11915794
Assignee: CHAKRABARTI ADVANCED TECHNOLOGY LLC
Field: Computer technology (Electrical engineering)
Classification: CPC G  C | IPC C  G

Claim 5:
6. The system of claim 5, wherein kf and kr are calculated according to Gibbs free energy for the annealing reaction and relaxation time for the annealing reaction, and wherein relationship between the Gibbs free energy, kf and kr is expressed as:, k
      f
    
    
      k
      r
    
  
  =
  
    
      [
      
        C
        o
      
      ]
    
    ⁢
    
      exp
      ⁡
      
        (
        
          
            
              -
              Δ
            
            ⁢
            
              
            
            ⁢
            G
          
          RT
        
        )
      
    
  

wherein [C0] is the standard concentration, R is the gas constant and T is the annealing reaction temperature and the relaxation time τ is expressed as:

τ=1/(kf[Peq+Seq]+kr)

wherein Peq and Seq are equilibrium concentrations of the primer and single strand DNA.