Patent ID: 11859203
Assignee: UCB BIOPHARMA SRL
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 21:
22. A method for producing a recombinant protein preparation comprising:
(i) inoculating said host cells in a basal medium, wherein the basal medium optionally comprises an initial amount of:
(a) cysteine/cystine analogs; and/or
(b) cysteine and/or cystine,
(ii) progressing the culture through a production phase wherein the recombinant protein is produced by the cells, wherein, during said production phase, the cell culture medium is supplemented with:
(a) cysteine/cystine analogs; and
(b) cysteine and/or cystine,
wherein (a) and (b) may be added simultaneously or sequentially,
wherein, when the contents of the basal medium and the total supplements added are added up, the molar ratio of (a) to (b) is between 1:10 and 10:1,
wherein the cysteine/cystine analogs are selected from the group consisting of N,N′-diacetyl-L-cystine-dimethylester, N-acetyl-L-cysteine and N,N′-diacetyl-L-cystine, wherein the recombinant proteins are antibodies, and wherein the host cells are mammalian cells.