Patent ID: 11866759
Assignee: SHENZHEN AGRECOE BIOTECHNOLOGY CO., LTD.
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 0:
1. A method of producing calcium propionate by using Lactobacillus reuteri, wherein the method comprises the following steps:
conducting a single base mutation on an alcohol dehydrogenase gene of the Lactobacillus reuteri by using a gene editing tool to obtain Lactobacillus reuteri having an inactivated alcohol dehydrogenase;
mutating the Lactobacillus reuteri having the inactivated alcohol dehydrogenase by an atmospheric room temperature plasma mutation technology to obtain a mutated Lactobacillus reuteri having an inactivated alcohol dehydrogenase;
mixing the mutated Lactobacillus reuteri having the inactivated alcohol dehydrogenase with 1,2-propanediol, then growing and reproducing the mutated Lactobacillus reuteri having the inactivated alcohol dehydrogenase, and then conducting culture of the mutated Lactobacillus reuteri having the inactivated alcohol dehydrogenase; and
after the culture, placing the mutated Lactobacillus reuteri having the inactivated alcohol dehydrogenase into a culture medium containing calcium ion for a fermentation culture, then obtaining the calcium propionate.