Patent ID: 11945842
Assignee: INTERNATIONAL CENTRE FOR GENETIC ENGINEERING AND BIOTECHNOLOGY (ICGEB)
Field: Pharmaceuticals (Chemistry)
Classification: CPC C  A  Y | IPC A  C

Claim 6:
7. A method for producing dengue virus like particles (VLPs) for preparation of a vaccine comprising:
(a) introducing an expression vector into cultured cells, wherein the expression vector comprises a nucleic acid encoding an EDIII-T polypeptide comprising an EDIII domain of each of Dengue virus serotypes DENY-1, DENV-2, DENV-3, and DENV-4, wherein the C-terminal end of EDIII-T is fused to the N-terminal end of an HBsAg polypeptide; and
(b) recovering the dengue VLPs.