Patent ID: 11926869
Assignee: INSTITUTE OF HORTICULTURE RESEARCH, ANHUI ACADEMY OF AGRICULTURAL SCIENCE
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 0:
1. A method for identifying a variety of a plant of a genus pomegranate, the method comprising:
extracting DNA from the plant;
conducting a polymerase chain reaction (PCR) using the extracted DNA and a Simple Sequence Repeat (SSR) primer group comprising 11 primer pairs, the 11 primer pairs comprise PG080. PG130, PG139, PG152, PG153, PG140, PG098, PG070, PG077, PG090, and PG093, wherein nucleotide sequences of each of the 11 primer pairs are as follows:, Sequence

ID
Primer
Forward primer

 1
PG080
ctgactgttgcagagagtaggctg

 

 3
PG130
ctcatatggcgattctctgtcctt

 

 5
PG139
gtttccttccctcaacccaaaa

 

 7
PG152
catcagaatcgtccccttgtg

 

 9
PG153
gtgtttgatgctcccatttcattt

 

11
PG140
caagaaagtgtgtgagcgattgat

 

13
PG098
tgccttcttaaggacttcaccaac

 

15
PG070
cacctctgcttcagcaaacaaata

 

17
PG077
gtcagtctcctccttcttcaatgg

 

19
PG090
attcttttatactaaccaaaatttgcga

 

21
PG093
cgtcaataggacgtccctgagata

Sequence

ID
Primer
Reverse primer

 2
PG080
aggaggtgaaacaacgaatagctg

 

 4
PG130
aagttcgataaattgcactggtgg

 

 6
PG139
agtgggattttaccaagtcgaaca

 

 8
PG152
cagagagaagaagagagaccgagc

 

10
PG153
gccttcaacggtctttcttcttct

 

12
PG140
ccaaaccttacccctctctctctc

 

14
PG098
ctaacctcatgcacttgtcatcca

 

16
PG070
caactcaacacaatatccaaccca

 

18
PG077
agacgaagcacctgagaaggaat

 

20
PG090
atgtcatgagaggacccacaaa

 

22
PG093
gatgacgtggcagagtaagagagc;

obtaining amplified DNA products;
analyzing SSR polymorphisms of the amplified DNA products; and
identifying the variety of the plant based on the polymorphic amplified DNA products.