Patent ID: 11965153
Assignee: REGENERON PHARMACEUTICALS, INC.
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 19:
20. A method of controlling a bioreactor system, comprising:
providing a cell culture in a perfusion bioreactor;
measuring one or more process parameters of the cell culture by a RAMAN probe;
removing cell-free spent media from the cell culture using a first output conduit at a first specified rate;
removing cells from the cell culture using a second output conduit at a second specified rate;
introducing one or both of fresh media or nutrients into the cell culture using an input conduit at a third specified rate;
changing one or more of the first specified rate, the second specified rate, or the third specified rate based on the RAMAN probe measurements, wherein a perfusion rate through the bioreactor system is maintained based on a constant; and
adjusting, based on the RAMAN probe measurements, the input and output conduits to maintain at least one of the process parameters within a predetermined range, the first and the second specified rates of each of the output conduits within respective predetermined ranges, the third specified rate of the input conduit within a predetermined range, and the perfusion rate through the bioreactor system based on the constant.