Patent ID: 11965179
Assignee: nan
Field: Pharmaceuticals (Chemistry)
Classification: CPC C  A | IPC A  C

Claim 0:
1. A method of creating a pooled platelet lysate product, the method comprising:
obtaining whole blood from a source:
purifying platelets from the whole blood and removing red blood cells, white blood cells, and plasma, thus separating the platelets from the whole blood to create concentrated platelets;
activating the concentrated platelets, causing the platelets to release cytokines and ATP; and
treating the activated concentrated platelets with a plurality of freeze/thaw cycles to destroy platelet membranes to create a platelet lysate,
wherein:
separating the platelets from the whole blood comprises using a pressurized rotating microfluidic filtration system; and
the pressurized rotating microfluidic filtration system includes an outer tube surrounding an inner tube and rotating relative to the inner tube, wherein the inner tube is porous and is configured to prevent transfer of larger sized molecules into the outer tube, thus separating the platelets from the whole blood.