Patent ID: 11892447
Assignee: LABORATOIRES EXPANSCIENCE
Field: Measurement (Instruments)
Classification: CPC G  C | IPC C  G

Claim 0:
1. A method for evaluating the in vitro efficacy of an active agent or of a formulation in reducing the effects of dehydration on children's skin, characterized by said method comprising the following steps:
a. incubating a reconstructed skin model under drying conditions, wherein the drying conditions comprises a relative humidity in the atmosphere of 25% or less;
b. contacting said active agent or said formulation with the reconstructed skin model of step a;
c. measuring the expression level of a combination of biological markers in the skin model of step b, wherein the biological markers comprise inflammation markers IL1A, IL8, PTGS2, NGFR, TAC1, TAC1R, TRPV1, TRPV3, MRGPRD, OSMR, PLA2G2F, and F2RL1; barrier function markers DSG, SCEL, POADI1, CASP14, LOR, TGM1, and CLDN1; and stem cell markers Tp63, KRT15, KRT19, BIRC5, and NOTCH1;
d. measuring the amounts of natural moisturizing factors (NMFs) and ceramides in the skin model of step b;
e. comparing the expression levels of the biological markers and the amounts of NMFs and ceramides to a control reconstructed skin model not exposed to drying conditions, wherein both the reconstructed skin model and the control reconstructed skin model are obtained from skin samples from a donor between newborn and 2 years, and determining that the agent or formulation is able to protect against dryness when the dried reconstructed skin model exposed to the agent or formulation has:
i. the same or a lower expression level of the following inflammation markers: IL1A, IL8, PTGS2, NGFR, TAC1, TAC1R, TRPV1, TRPV3, MRGPRD, OSMR, PLA2G2F, and F2RL1 when compared to the control reconstructed skin model;
ii. the same or a higher expression level of the following barrier function markers: DSG, SCEL, PAD1L, CASP14, LOR, TGM1, and CLDN1 when compared to the control reconstructed skin model;
iii. the same or a higher expression level of the following stem cell markers: Tp63, KRT15, KRT19, BIRC5, and NOTCH1 when compared to the control reconstructed skin model;
iv. the same or a higher amount of NMFs and ceramides.