Patent ID: 11946068
Assignee: EPIBONE, INC.
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 13:
14. A method for seeding and culturing therapeutic active ingredients (TAIs) into a porous scaffold, comprising:
accommodating the porous scaffold in a first chamber;
delivering TAIs, TAI media and gas to the porous scaffold via a flow circuit from a TAI storage device, at least one second chamber and a gas inlet;
pumping at least one of the TAIs, the TAI media and the gas in the flow circuit by a pump; and
regulating a delivery of the TAIs, the TAI media and the gas to the porous scaffold by a processor via a plurality of valves,
wherein the plurality of valves include:
a media valve for regulating the delivery of the TAI media from the second chamber;
a gas valve for regulating the delivery of the gas from the gas inlet
a TAI valve for regulating the delivery of the TAIs from the TAI storage device;
a first chamber valve for regulating the delivery of the gas to the first chamber; and
a waste valve for regulating a flow from the first chamber; and

wherein the processor performs a pre-wetting process for pre-wetting the porous scaffold with the TAI media, comprising steps of:
(i) setting the media valve, the first chamber valve, and the waste valve to an open position while the gas valve and the TAI valve are in a closed position to pump the TAI media into the first chamber;
(ii) setting the gas valve, the first chamber valve, and the waste valve to an open position while the media valve and the TAI valve are closed to pump the gas into the first chamber; and
(iii) regulating pulsing pressurized gas bursts through the porous scaffold by repeatedly alternating each of the gas valve, the first chamber valve and the waste valve between an open position and a closed position while the media valve and the TAI valve are in a closed position.