Patent ID: 11905310
Assignee: UCB BIOPHARMA SRL
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 0:
1. A process for the purification of a protein of interest from a mixture comprising the steps of
a) in an operational chromatography cycle loading a first volume of a mixture containing the protein of interest from a first container to a chromatography matrix operated such that the protein of interest binds to the chromatography matrix until 40% to 100% of the maximum static binding capacity of the chromatography matrix for the protein of interest is reached;
b) collecting flow-through containing unbound protein of interest in a second container, and
c) in a further operational chromatography cycle re-loading the flow-through collected in the second container to the same chromatography matrix followed by loading a second volume of the protein of interest from the first container to the same chromatography matrix, operated such that the protein of interest binds to the chromatography matrix until 40% to 100% of the maximum static binding capacity of the chromatography matrix for the protein of interest is reached.