Patent ID: 11965181
Assignee: RIVER STONE BIOTECH, INC.
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 0:
1. A recombinant yeast cell capable of producing one or more benzylisoquinoline alkaloids or benzylisoquinoline alkaloid precursors, or both, comprising:
(a) reduced or eliminated enzymatic activity of Genes de Respuesta a Estres 2 (GRE2) comprising the amino acid sequence of SEQ ID NO: 7, or an amino acid sequence having at least 90% identity to SEQ ID NO: 7; and, optionally,
(b) reduced or eliminated enzymatic activity of one or more alcohol dehydrogenases or other aldehyde reductases, or a combination thereof,, wherein the activity of each of the enzymes in (a) and (b) is reduced or eliminated, and whereby the recombinant yeast cell is thereby capable of increased production of one or more benzylisoquinoline alkaloids or benzylisoquinoline alkaloid precursors, or both, than are produced in cells without reduced or eliminated activity of the enzymes.