Patent ID: 11858832
Assignee: INGURAN, LLC
Field: Measurement (Instruments)
Classification: CPC C  G | IPC C  G

Claim 24:
25. A method, comprising:
flowing cells through a measurement region at differing positions relative to an optical axis of collection optics;
generating excitation electromagnetic radiation;
directing the excitation electromagnetic radiation toward cells in the measurement region, where the cells emanate output electromagnetic radiation in response to the excitation electromagnetic radiation;
collecting the output electromagnetic radiation from the cells;
generating an at least one electrical signal responsive to the intensity of the collected output electromagnetic radiation and responsive to a cell's position passing through the measurement region relative to the optical axis of the collection optics;
normalizing the intensity of the output electromagnetic radiation represented in the electrical signal by adjusting the detected intensity of the output electromagnetic radiation based on the position of the cell at the measurement region relative to the optical axis of the collection optics, and
discriminating a first type of cell from other cells with the adjusted output electromagnetic radiation intensity.