Patent ID: 11932902
Assignee: ROCHE SEQUENCING SOLUTIONS, INC.
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 0:
1. A method for adding cell origination barcodes onto beads, comprising:
(a) splitting a pool of beads into a plurality of reaction volumes;
(b) appending pre-made oligonucleotides onto the beads in the plurality of reaction volumes, wherein at least some of the plurality of reaction volumes each receive a pre-made oligonucleotide that contains a sequence that is different from the other pre-made oligonucleotides added to the plurality of reaction volumes;
(c) pooling the beads; and
(d) repeating steps (a)-(c) one or more times to produce a pool of beads that comprise the cell origination barcodes,
wherein in step (d) the oligonucleotides that are appended in the one or more repeats of step (b) are added to previously appended oligonucleotides to form the cell origination barcodes.