Patent ID: 11913036
Assignee: CODEXIS, INC.
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 2:
3. The engineered acetate kinase of claim 1, wherein said polypeptide sequence of said engineered acetate kinase has at least 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, or 99% sequence identity to SEQ ID NO: 2, and wherein said polypeptide sequence of said engineered acetate kinase further comprises at least one substitution or substitution set at one or more positions selected from 4/142/242, 12/76/164, 15, 23, 39, 41, 44, 45, 47, 47/411, 48, 50, 51, 53, 55, 57/135, 58, 58/135, 61, 76, 76/142/299/386, 76/164, 76/164/232/262/386, 76/164/262, 76/164/273, 76/164/287/364, 76/164/386, 76/232, 76/232/259/364, 76/232/262, 76/232/273/299/311/386, 76/232/364/386, 76/262, 76/262/273, 76/262/284/287/386, 76/262/284/364, 76/262/287, 76/262/364, 76/273, 76/284/299, 76/284/311/386, 76/364, 76/386, 130, 132, 135/284, 135/392, 145/400, 164/232/284/287, 191, 220, 220/360, 241, 246, 262/284/287, 273/311/313/364, 275, 279, 283, 285, 287, 288, 289, 290, 292, 297, 298, 298/405, 299, 311, 311/364/386, 332, 340, 343, 343/388, 347, 364, 372, 373, 374, 375, 376, 391, 392, and 405, wherein the amino acid positions of said polypeptide sequence are numbered with reference to SEQ ID NO: 2.