Patent ID: 11913942
Assignee: IDEXX LABORATORIES, INC.
Field: Measurement (Instruments)
Classification: CPC G  C | IPC C  G

Claim 2:
3. The method of claim 1, further comprising
(a) conducting a calibrator first reaction sequence comprising:
i. forming calibrator first reaction mixtures by individually contacting each calibrator of a set of calibrators comprising known amounts of the analyte with an anti-analyte antibody, a conjugate comprising the analyte and an enzyme, and a substrate that produces a signal when in contact with the enzyme, and
ii. measuring signal from each of the calibrator first reaction mixtures;

(b) conducing a calibrator second reaction sequence comprising,
i. forming calibrator second reaction mixtures by contacting each calibrator of the set of calibrators with the substrate, and
ii. measuring signal from the calibrator second reaction mixtures;

(c) subtracting an amount of signal from step (a) from an amount of signal from step (b) to provide a net signal for each of the calibrators,
(d) using the net signal from two or more of the calibrators to generate a standard curve,
(e) determining the amount of the analyte in the sample by comparing the net signal from the sample to the standard curve.