Patent ID: 11965183
Assignee: OXFORD NANOPORE TECHNOLOGIES PLC
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 13:
14. The method of claim 1, wherein the Dda helicase further comprises in (i) a substitution of at least one additional amino acid which interacts with one or more phosphate groups in one or more nucleotides in single stranded DNA (ssDNA),
wherein the substitution of at least one amino acid which interacts with one or more phosphates in one or more nucleotides in ssDNA:
increases the (a) electrostatic interactions, (b) hydrogen bonding and/or (c) cation-pi (cation-π) interactions between the at least one amino acid and the one or more phosphate groups in ssDNA,
increases the net positive charge of the position, and/or
is with a non-natural amino acid.