Patent ID: 11905548
Assignee: WATERS TECHNOLOGIES CORPORATION
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 0:
1. An on-line immobilized enzymatic apparatus comprising:
a wall defining a chamber having an inlet and an outlet;
a solid stationary phase comprising nonporous particles covalently linked to an enzyme and disposed within the chamber, wherein the nonporous particles have a mean size within the range of 1 and 10 microns and provide support for digestion by the enzyme at chamber pressures between 2,000 and 15,000 psi; and
a pressure modulator in fluid communication with the chamber and adapted to support on- line flow of a liquid sample comprising a polymer analyte through the inlet, over the solid stationary phase, and out of the outlet, wherein nonporous particles have a core composition and a surface composition represented by Formula 1:

W—[X]-Q  Formula 1, wherein X is core composition having a surface comprising a silica core material, a metal oxide core material, an organic-inorganic hybrid core material or a group of block polymers thereof; W is hydrogen or hydroxyl; and Q is absent or is a functional group that minimizes electrostatic interactions, Van der Waals interactions, hydrogen-bonding interactions or other interactions with the analyte, and wherein the enzyme is covalently linked to the nonporous particles by means of a covalent linker comprising an organo-functionalize silane linker.