Patent ID: 11898197
Assignee: WAINAMICS, INC.
Field: Chemical engineering (Chemistry)
Classification: CPC C  B | IPC B  C

Claim 0:
1. A device for performing a bioassay on a biological sample to determine the presence or quantity of one or more target polynucleotides when connected to an appliance that provides pressure and vacuum sources and a detection station, the device comprising:
a planar body comprising a sample chamber, a first conduit, at least one reagent chamber, at least one metering chamber, at least one mixing chamber and at least one detection chamber, wherein:
the sample chamber has a first inlet accepting a biological sample or a sample fluid containing a biological sample, a vent port allowing the passage of air but not liquid, and an outlet connected to the first conduit, wherein the vent port is capable of being sealingly connected to a valve in the appliance;
the metering chamber (a) connected to and in fluid communication with a reagent chamber, (b) connected to and in fluid communication with the outlet of the sample chamber through the first conduit, (c) connected to a metering vent port, and (d) connected to and in fluid communication with a mixing chamber conduit, wherein the metering vent port is capable of being sealingly connected to a valve in the appliance;
the reagent chamber capable of containing assay reagents, the reagent chamber being connected to the metering chamber by a passage and connected to a reagent vent port allowing the passage of air but not liquid, wherein the reagent vent port is capable of being sealingly connected to a valve and pump in the appliance so that the reagent port is capable of accepting air pressure for forcing the assay reagents into the metering chamber;
the mixing chamber for mixing the biological sample or sample fluid with the assay reagents, the mixing chamber having a top and a bottom and being in fluid communication with the metering chamber by a passage connecting the bottom of the mixing chamber to the metering chamber, the mixing chamber being connected at its top to a mixing vent port that allows the passage of air but not liquid, wherein the mixing vent port is capable of being sealingly connected to a valve in the appliance, wherein the mixing chamber or the reagent chamber or both chambers contain a predetermined quantity of wax having a melting temperature such that the wax forms a bubble-preventing layer on a reaction mixture whenever the mixing chamber is above the melting temperature; and
the detection chamber is in fluid communication with the mixing chamber by a passage connecting the detection chamber to the bottom of the mixing chamber, and the detection chamber is connected at its top to a detection vent port that allows the passage of air but not liquid, wherein the detection vent port is capable of being sealingly connected to a valve and vacuum source in the appliance so that the detection port is capable of accepting a vacuum for drawing the mixture of assay reagents and biological sample or sample fluid into the detection chamber from the mixing chamber.