Patent ID: 11944973
Assignee: SHARP LIFE SCIENCE (EU) LIMITED
Field: Measurement (Instruments)
Classification: CPC B  G | IPC B  G

Claim 0:
1. A microfluidic system comprising:
an electro-wetting on dielectric (EWOD) device comprising an element array configured to receive liquid droplets, the element array comprising a plurality of individual array elements and wherein one of said liquid droplets comprises a fluid reservoir containing particles that is inputted into the EWOD device;
a detector for detecting a property of a liquid droplet dispensed onto the element array; and
a control system configured to perform electrowetting operations by controlling actuation voltages applied to the element array to perform manipulation operations as to liquid droplets present on the element array;
wherein the control system is configured to perform the steps of:
performing an electrowetting operation to dispense a plurality of dispensed droplets from the fluid reservoir, the dispensed droplets having a Poisson distribution of droplets containing a desired number of particles;
interrogating each dispensed droplet with the detector and determining whether each dispensed droplet has the desired number of particles;
selecting dispensed droplets that contain the desired number of particles and performing an electrowetting operation to move the selected dispensed droplets to a reaction area on the EWOD device, the selected dispensed droplets having a non-Poisson distribution of droplets containing the desired number of particles; and
rejecting dispensed droplets that do not contain the desired number of particles and performing an electrowetting operation to move the rejected dispensed droplets to a holding area on the EWOD device that is different and spaced apart from the reaction area.