Patent ID: 11858981
Assignee: NOVARTIS AG
Field: Pharmaceuticals (Chemistry)
Classification: CPC C  A | IPC A  C

Claim 18:
19. A method for making an immunobinder, the immunobinder comprising rabbit heavy chain CDR H1, CDR H2 and CDR H3 sequences and light chain CDR L1, CDR L2 and CDR L3 sequences, the method comprising:
(i) grafting the rabbit CDR H1, CDR H2 and CDR H3 sequences into a heavy chain variable domain that comprises human heavy chain framework sequences that are at least 85% identical to the heavy chain framework sequences of SEQ ID NO: 4, and that comprises a threonine (T) at position 24 according to AHo numbering, and
(ii) grafting the rabbit CDR L1, CDR L2 and CDR L3 sequences into a light chain variable domain that comprises human light chain framework sequences that are at least 85% identical to the light chain framework sequences of SEQ ID NO: 2.