Patent ID: 11859245
Assignee: INSTITUTE OF ZOOLOGY, CHINESE ACADEMY OF SCIENCES
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 12:
13. The method of claim 6, wherein the CHAPS lysis buffer comprises 15 mM of Tris-HCl (pH 7.5), 2 mM of MgCl2, 1.5 mM of ethylene glycol tetraacetic acid (EGTA), 0.75% of CHAPS (m/v), 15% of glycerol (v/v), 7.5 mM of dithiothreitol (DTT), 0.75 mM of a protease inhibitor 4-(2-aminoethyl)benzenesulfonyl fluoride hydrochloride (AEBSF), 1.5 U/μL of a RNase inhibitor, and 0.6 mg/mL of BSA.