Patent ID: 11939562
Assignee: PLURI BIOTECH LTD.
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 9:
10. A method for large scale expansion of immune cells in a three-dimensional (3D) bioreactor, the method comprising the steps of:
a. inserting at least one porous scaffold into at least one packed bed chamber;
b. coating said at least one porous scaffold with one or more extra cellular matrix protein (ECM);
c. circulating a fluid media having at least one population of immune cells suspended in said fluid media contained in at least one container surrounding said at least one packed bed chamber, said fluid media having at least one population of immune cells suspended in said fluid media flows through said at least one packed bed chamber and said at least one porous scaffold coated with one or more ECM; and
wherein, said at least one porous scaffold coated with one or more ECM creates a stationary niche having low shear forces that imitate the natural growth environment of said population of immune cells; and
wherein said at least one porous scaffold coated with one or more ECM has a porosity which allows expansion of said at least one population of immune cells that flows through said at least one porous scaffold coated with one or more ECM in large scale without immobilizing said at least one population of immune cells.