Patent ID: 11879138
Assignee: CK-EXOGENE CO., LTD.
Field: Pharmaceuticals (Chemistry)
Classification: CPC C  A | IPC A  C

Claim 0:
1. A method for preparing mesenchymal stem cell-derived exosomes, the method comprising: obtaining a cell sample from mesenchymal stem cells;
sub-culturing the cell sample in a low-glucose Dulbecco's Modified Eagle's Medium (DMEM) medium;
culturing the sub-cultured cells in a substrate medium containing a protein synthesis enzyme inhibitor, and then obtaining a cell culture solution; and
isolating exosomes from the cell culture solution,
wherein in the obtaining of the cell culture solution, the substrate medium further comprises TNFα,
wherein the protein synthesis enzyme inhibitor is any one or more selected from the group consisting of cycloheximide, anisomycin, aurintricarboxylic acid, diphtheria toxin, edeine, fusidic acid, pactamycin, puromycin, ricin, sodium fluoride, sparsomycin, tetracycline, and Trichoderma, and
wherein the substrate medium has a TNFα: protein synthesis inhibitory enzyme ratio of 1:10 to 1:2000.