Patent ID: 11866745
Assignee: PAIRWISE PLANTS SERVICES, INC.
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 0:
1. A modified Lachnospiraceae bacterium CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) Cas12a (LbCas12a) polypeptide, wherein the modified LbCas12a polypeptide comprises an amino acid sequence having at least 95% identity to the amino acid sequence of SEQ ID NO:1 (LbCas12a) and a mutation of K595Y with reference to position numbering of SEQ ID NO:1, wherein the modified LbCas12a polypeptide exhibits a reduced PAM stringency and increased recognition of new protospacer adjacent motifs (PAMs) as compared to wild type LbCas12a, SEQ ID NO:1.