Patent ID: 11867649
Assignee: NANO HESGARSAZAN SALAMAT ARYA NCUBATION CENTER FOR MEDICAL EQUIPMENT AND DEVICES
Field: Measurement (Instruments)
Classification: CPC G  B | IPC B  G

Claim 9:
10. The method of claim 8, wherein forming the two-phase mixture of the biological sample in the solution of chloroform and methanol comprises:
absorbing secretion of the biological sample by keeping the biological sample on a foam for a time period between 5 minutes and 30 minutes;
forming a mixture of chloroform and the secretion of the biological sample by putting the foam containing the secretion of the biological sample in a chloroform solution inside a shaker for a time period between 5 minutes and 30 minutes;
removing the foam from the mixture of chloroform and the secretion of the biological sample; and
adding methanol to mixture of chloroform and the secretion of the biological sample with a volume ratio of 1:2 (Chloroform:methanol).