Patent ID: 11913905
Assignee: UNIVERSITY OF WASHINGTON
Field: Measurement (Instruments)
Classification: CPC G  B  C  Y | IPC B  C  G

Claim 0:
1. A method of detecting two or more nucleotides of an analyte comprising a nucleic acid, the method comprising:
(a) providing a nanopore positioned between a cis side comprising a first conductive liquid medium and a trans side comprising a second conductive liquid medium, wherein the nanopore provides liquid communication between the cis side and the trans side, wherein the analyte is present on the cis side, the analyte comprising a first arresting construct;
(b) causing the analyte that comprises the first arresting construct to advance into the nanopore, whereby the first arresting construct pauses the advance of the analyte toward the trans side, thereby producing a first residual ion current level that represents a first nucleotide or set of nucleotides adjacent to the first arresting construct, wherein the first residual ion current level is different than a measurable ion current of the same nucleotide or set of nucleotides in an analyte that is not arrested;
(c) altering the first arresting construct of the analyte, the alteration allowing the analyte to advance toward the trans side;
(d) causing a second arresting construct to pause the advance of the analyte toward the trans side, thereby producing a second residual ion current level that represents a second nucleotide or set of nucleotides adjacent to the second arresting construct; and
(e) detecting a difference between the first residual ion current level and the second residual ion current level to distinguish the first nucleotide or set of nucleotides from a second nucleotide or set of nucleotides.