Patent ID: 11965009
Assignee: TRUSTEES OF BOSTON UNIVERSITY
Field: Pharmaceuticals (Chemistry)
Classification: CPC C  A | IPC A  C

Claim 4:
5. A method of making a protein comprising:
(a) providing a DNA expression vector comprising:
(i) a toxP;
(ii) a mutant toxO that blocks Fe-mediated regulation of gene expression;
(iii) a signal sequence, and
a DNA sequence encoding a protein;

(b) transforming a bacteria strain with the DNA expression vector to form a transformant;
(c) incubating the transformant in a culture medium for a period of time to allow expression of a protein that is secreted into the culture medium; and
(d) purifying the protein from the culture medium,
wherein the protein is selected from any one of SEQ ID NO:12, SEQ ID NO: 14 and SEQ ID NO:15.