Patent ID: 11866780
Assignee: ILLUMINA CAMBRIDGE LIMITED
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 0:
1. A composition comprising: an array comprising a plurality of nucleic acid features randomly distributed on a surface, wherein each nucleic acid feature comprises a pair of polynucleotides and a single stranded target nucleic acid hybridized to the pair of polynucleotides, wherein the pair of polynucleotides comprises:
(i) a first polynucleotide immobilized on the surface at a first location, wherein the first polynucleotide comprises: a first universal sequence common to all features, and a first capture region capable of hybridizing to a first target region of the single-stranded target nucleic acid;
(ii) a second polynucleotide immobilized on the surface at a second location via hybridization to a capture probe attached to the surface, wherein the second polynucleotide comprises: a second universal sequence common to all features, and a second capture region capable of hybridizing to a second target region of the single-stranded target nucleic acid, wherein the first target region is non-contiguous with the second target region in the single-stranded target nucleic acid; and
wherein the single-stranded target nucleic acid is single-stranded between the first target region hybridized to the first capture region and the second target region hybridized to the second capture region.