Patent ID: 11862299
Assignee: GEN-PROBE INCORPORATED
Field: Computer technology (Electrical engineering)
Classification: CPC G | IPC G

Claim 0:
1. A method of differentially treating a patient population, comprising;
sequencing samples from members of the patient population; wherein for each sample the sequencing comprises:
(i) ligating a target nucleic acid and an anchor segment of known sequence and thereby forming a nucleic acid target template, which is a circular molecule in which the target nucleic acid forms an adjacent segment adjacent the anchor segment, (ii) generating raw target sequencing reads by synthesis directed by a polymerase reading around the nucleic acid target template multiple times primed from a primer binding to the anchor segment, the raw target sequencing reads comprise alternating reads of the anchor segment and the target nucleic acid; and at least some of the raw target sequencing reads containing sequencing errors; (iii) performing the following computer-implemented steps:
(a) receiving a population of raw target sequencing reads of the nucleic acid target template;
(b) evaluating the accuracy of sequencing of the adapter segment in different raw target sequences by comparing raw target sequencing reads of the anchor segment with the known correct sequence of the adapter segment;
(c) assigning a subset of the raw target sequences into an accepted class based on the accuracy of sequencing of the adapter segment in the raw target sequences;
(d) aligning at least some of the raw target sequences from the accepted class; and
(e) determining a sequence of at least part of the adjacent segment from the aligned sequences;
wherein different members of the patient population receive different treatment regimes depending on the determined sequence for the sample from each member.