Patent ID: 11959135
Assignee: OXFORD NANOPORE TECHNOLOGIES PLC
Field: Measurement (Instruments)
Classification: CPC C  B  G | IPC B  C  G

Claim 0:
1. A method for enhancing a target polynucleotide concentration on a surface during nanopore sequencing, the method comprising:
(i) contacting a target polynucleotide construct to a surface comprising a nanopore, and a solid-state membrane supporting a lipid bilayer, wherein the target polynucleotide construct comprises:
(a) a single stranded DNA (ssDNA) leader;
(b) a target polynucleotide; and
(c) one or more reactive groups that interact with the lipid bilayer, under conditions under which the one or more reactive groups interacts with the lipid bilayer and tethers the target polynucleotide construct to the lipid bilayer resulting in enhanced concentration of the target polynucleotide on the surface in proximity to the nanopore; and

(ii) detecting the target polynucleotide using the nanopore.