Patent ID: 11951155
Assignee: ZHONGSHI DUQING(SHANDONG) BIOTECH CO., LTD
Field: Pharmaceuticals (Chemistry)
Classification: CPC A | IPC A

Claim 0:
1. A method for preparing a pea-derived peptide comprising one or more of the following polypeptides as separate peptide fragments: PP1 with an amino acid sequence shown in SEQ ID NO: 1, PP2 with an amino acid sequence shown in SEQ ID NO: 2, PP3 with an amino acid sequence shown in SEQ ID NO: 3, PP4 with an amino acid sequence shown in SEQ ID NO: 4, and PP5 with an amino acid sequence shown in SEQ ID NO: 5, wherein the method comprises the following steps:
1) conducting enzymatic hydrolysis on a pea protein under an action of an alkaline protease to obtain a first enzymatic hydrolysate;
2) conducting enzymatic hydrolysis on the first enzymatic hydrolysate under an action of papain and cellulase to obtain a second enzymatic hydrolysate;
3) inactivating the second enzymatic hydrolysate, then filtering inactivated second enzymatic hydrolysate through a ceramic membrane, filtering through an organic membrane, and collecting a permeate to obtain a pea-derived peptide;
4) subjecting the pea-derived peptide to gel chromatography, and collecting a fraction S2 12-18 min;
5) subjecting the fraction S2 to separation and purification by preparative liquid chromatography, and collecting a fraction W5 at 55-63 min; and
6) subjecting the fraction W5 to separation and purification by analytical liquid chromatography, and sequentially collecting 5 polypeptide fragments at a PP1 time interval, a PP2 time interval, a PP3 time interval, a PP4 time interval, and a PP5 time interval, respectively, to obtain PP1, PP2, PP3, PP4, and PP5.