Patent ID: 11927737
Assignee: CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE
Field: Measurement (Instruments)
Classification: CPC G | IPC G

Claim 6:
7. A device for the characterization of the 3D orientation of at least one emitting dipole within a specimen, wherein said device is configured to be connected to a microscope system comprising a microscope objective lens with a given numerical aperture and a detection unit having a detection plane optically conjugated with a front focal plane of said microscope objective lens, the device comprising:
A non-polarizing beam splitter configured to split a light beam emitted by said at least one emitting dipole of the specimen positioned in a front focal plane of said microscope objective lens and exiting said objective lens, into a first and a second beams, wherein the first beam is directed to a first detection channel and the second beam is directed to a second detection channel;
a spatial frequency filter arranged in the first detection channel, configured for spatially filtering said first beam to select a given range of spatial low frequencies of said first beam, wherein said spatial frequency filter is configured to be positioned in a filtering plane conjugated with a back focal plane of said microscope objective lens when said device is connected to said microscope system;
in each of said first and second detection channels, respectively a first polarizing beam splitter and a second polarizing beam splitter, wherein said first and second polarizing beam splitters are configured to split each of said filtered first beam and second beam into two beams linearly polarized, thus providing four beams linearly polarized having four different directions of polarization;
a calculation unit configured to determine, from four intensity images resulting from the detection of said four beams linearly polarized by the detection unit in said detection plane, the mean orientation and the angular aperture of the distribution of the 3D orientation of said at least one emitting dipole, in a predefined frame of the specimen, during an acquisition time of said four images.