Patent ID: 11884024
Assignee: ROCHE SEQUENCING SOLUTIONS, INC.
Field: Measurement (Instruments)
Classification: CPC B  C  G  Y | IPC B  C  G

Claim 6:
7. A system for identifying a base of a nucleic acid molecule, the system comprising:
a sensing circuit configured to be coupled to a nanopore;
an electrical circuit configured to apply an electrical stimulus across the nanopore; and
a processor coupled to the sensing circuit and the electrical circuit, wherein the processor is programmed to:
control the electrical circuit to apply a first progression electrical stimulus across the nanopore, the first progression electrical stimulus having a first polarity;
control the sensing circuit to record a first electrical measurement indicative of a plurality of bases of the nucleic acid molecule as a strand of the nucleic acid molecule moves through the nanopore in a first direction as a result of the application of the first progression electrical stimulus;
control the electrical circuit to apply a first reverse progression electrical stimulus across the nanopore, the first reverse progression electrical stimulus having a second polarity that is opposite of the first polarity such that the nucleic acid molecule moves through the nanopore in a second direction that is opposite the first direction;
control the electrical circuit to apply a second progression electrical stimulus across the nanopore, the second progression electrical stimulus having the first polarity;
control the sensing circuit to record a second electrical measurement indicative of the plurality of bases of the nucleic acid molecule as the strand of the nucleic acid molecule moves through the nanopore in the first direction as a result of the application of the second progression electrical stimulus; and
identify the base based on the first electrical measurement and the second electrical measurement.