Patent ID: 11857605
Assignee: BILLION KING INTERNATIONAL LIMITED
Field: Pharmaceuticals (Chemistry)
Classification: CPC A | IPC A

Claim 20:
21. A method for preparing the thiosuccinyl-crosslinked hemoglobin conjugate of claim 1, the method comprising:
contacting a tetrameric hemoglobin with a fumaryl crosslinking agent thereby forming a fumaryl-crosslinked hemoglobin: contacting the fumaryl-crosslinked hemoglobin with a thiol or a pharmaceutically acceptable salt or zwitterion thereof thereby forming a thiosuccinyl-crosslinked hemoglobin; and contacting the thiosuccinyl-crosslinked hemoglobin with a reactive water-soluble polymer reagent comprising a water-soluble polymer, a reactive functional group and optionally a linker, wherein the linker is covalently attached to the water-soluble polymer and the reactive functional group, thereby forming the thiosuccinyl-crosslinked hemoglobin conjugate.