Patent ID: 11873504
Assignee: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 7:
8. An isolated eukaryotic cell comprising one or more of:
a) a CasX polypeptide, or a nucleic acid molecule encoding the CasX polypeptide,
b) a CasX fusion polypeptide, or a nucleic acid molecule encoding the CasX fusion polypeptide, wherein the CasX fusion polypeptide comprises a CasX polypeptide fused to a heterologous polypeptide, and
c) a CasX guide RNA, or a nucleic acid molecule encoding the CasX guide RNA, wherein the CasX guide RNA comprises (i) a targeter sequence comprising a guide sequence that hybridizes to a target nucleic acid, and a duplex-forming segment; and (ii) an activator sequence that hybridizes with the duplex-forming segment of the targeter sequence to form a double stranded RNA(dsRNA) duplex that binds the CasX polypeptide and guides the bound CasX polypeptide to the target nucleic acid.