Patent ID: 11867661
Assignee: SAGE SCIENCE, INC.
Field: Measurement (Instruments)
Classification: CPC G  C | IPC C  G

Claim 0:
1. A method, comprising:
providing an electrophoresis cassette comprising:
at least one sample well,
at least one gel column containing a separation gel, and
an elution module array comprising a plurality of elution modules arranged adjacent the at least one gel column;

isolating high-molecular weight (HMW) DNA from a sample;
enzymatically cleaving the HMW DNA isolated from the sample within single-copy DNA sequences on both sides of a genomic DNA region that contains a simple sequence repeat region (“region”) to produce a cleaved sample;
fractionating the cleaved sample by gel electrophoresis through the separation gel;
electroeluting the fractionated DNA from the separation gel into the plurality of elution modules arranged adjacent to the at least one gel column, thereby creating a plurality of size fractions from the cleaved sample;
subjecting the size fractions to qPCR assays to detect single-copy sequences within the genomic fragments that also carry the region;
determining the length of a size of the size fractions showing a positive qPCR signal from their position within the elution module array;
and
determining the length of the region in the size fractions showing a positive qPCR signal and the known positions of the enzymatic cleavage sites.