Patent ID: 11879830
Assignee: ARIZONA BOARD OF REGENTS ON BEHALF OF THE UNIVERSITY OF ARIZONA
Field: Measurement (Instruments)
Classification: CPC G | IPC G

Claim 12:
13. A method for determining the presence of a target in a sample, comprising the steps of:
providing an imaging system including a lens-free holographic microscope comprising a light source in a first plane spaced above an image sensor, wherein the image sensor extends in a second plane, a microfluidic chip positioned between the light source and the image sensor, wherein the microfluidic chip extends in a third plane, which is parallel to the second plane, at least one chamber in the microfluidic chip, and a plurality of functionalized beads, any two of the plurality of functionalized beads with an affinity for binding together when exposed to the target, wherein the plurality of functionalized beads are positioned within the at least one chamber in the microfluidic chip;
adding the sample to the at least one chamber in the microfluidic chip;
directing the light source toward the microfluidic chip and the image sensor;
capturing, via the image sensor, an interference pattern generated by light scattered by the sample;
generating a hologram image based on the interference pattern; and
reconstructing the hologram image to generate an in-focus image.