Patent ID: 11859254
Assignee: MAYO FOUNDATION FOR MEDICAL EDUCATION AND RESEARCH
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 0:
1. A method comprising:
measuring a methylation level of a CpG site for three or more markers in a biological sample of a human individual through
treating genomic DNA in the biological sample with bisulfite;
amplifying the bisulfite-treated genomic DNA using primers specific for a CpG site for each of the three or more markers,
determining the methylation level of the CpG site for the three or more markers by methylation-specific PCR, quantitative methylation-specific PCR, methylation-sensitive DNA restriction enzyme analysis, quantitative bisulfite pyrosequencing, or bisulfite genomic sequencing PCR,
wherein the three or more markers are selected from ELMO1, ZNF569, C13orf18, CD1D, ARHGEF4, SFMBT2, PPP2R5C, CYP26C1, PKIA, CLEC11A, LRRC4, ST8SIA1, NDRG4 and FER1L4, wherein the three or more markers at least include SFMBT2, FER1L4, and ARHGEF4.