Patent ID: 11866713
Assignee: DANISCO US INC.
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 0:
1. A Bacillus licheniformis cell comprising:
1) A rghR2 gene encoding a RghR2 protein comprising at least 90% sequence identity to SEQ ID NO: 4, wherein the rghR2 gene contains an 18-nucleotide duplication encoding a repeat of amino acids AAAISR at amino acid positions 38-43 of the RghR2 protein of SEO ID NO: 4, and
2) A targeting vector configured to delete the 18-nucleotide duplication in the rghR2 gene, which upon deletion of the 18-nt duplication results in a modified cell comprising a modified rghR2 gene encoding a RghR2 protein comprising at least 90% sequence identity to SEQ ID NO: 2, wherein the modified cell produces an increased amount of a protein of interest relative to an unmodified cell.