Patent ID: 11932902
Assignee: ROCHE SEQUENCING SOLUTIONS, INC.
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 17:
18. The method of claim 17, wherein the split-pool process comprises:
(a) providing beads that are tethered to nucleic acids, wherein the beads are in a plurality of reaction volumes and at least some of the reaction volumes comprises multiple beads;
(b) appending pre-made oligonucleotides to the nucleic acids in the plurality of reaction volumes, wherein at least some of the reaction volumes each receive a different oligonucleotide;
(c) pooling the beads of (b) to produce a pool,
(d) splitting the pool of (c) into a plurality of reaction volumes, wherein at least some of the volumes receive multiple beads; and
(e) appending additional pre-made oligonucleotides to nucleic acids on the beads in the plurality of reaction volumes of (d);
wherein in step (e) at least some of the reaction volumes each receive a different pre-made oligonucleotide and the pre-made oligonucleotides are appended to products of step (b).