Patent ID: 11879152
Assignee: WILLIAM MARSH RICE UNIVERSITY
Field: Organic fine chemistry (Chemistry)
Classification: CPC C | IPC C  G

Claim 0:
1. A method for providing or preparing a nucleic acid probe for selective capture or enrichment of a nucleic acid molecule bearing a target nucleic acid sequence with a desired yield, comprising in order:
contacting a first sample containing the nucleic acid molecule bearing the target nucleic acid sequence with a test solution comprising the nucleic acid probe comprising a first concentration of a first nucleic acid molecule and a second concentration of a second nucleic acid molecule at a temperature and a buffer condition conducive to hybridization of the target nucleic acid sequence to the first nucleic acid molecule, wherein the second concentration is greater than the first concentration, wherein the first nucleic acid molecule comprises a first probe subsequence and a second probe subsequence, wherein the first probe subsequence is complementary to and sufficient to hybridize to a first target subsequence of the nucleic acid molecule and the second probe subsequence is complementary to and sufficient to hybridize to a second target subsequence of the nucleic acid molecule, wherein at least a portion of the target nucleic acid sequence is contained within the first target subsequence, and wherein the second nucleic acid molecule comprises a third probe subsequence that is complementary to and sufficient to hybridize at least a subsequence of the first probe subsequence and is not sufficient to hybridize to the second probe subsequence;
determining an experimental yield, wherein the experimental yield is the proportion of the target nucleic acid sequence in the first sample that is hybridized to the first nucleic acid molecule;
determining a third concentration of the second nucleic acid molecule, wherein the third concentration [P]′0 is determined by Equation 1, wherein [P]0 is the second concentration, wherein χ1 is the experimental yield, and wherein χ2 is the desired yield, wherein the desired yield is the desired proportion of the target nucleic acid sequence that is hybridized to the first nucleic acid molecule; and
providing instructions to use the first concentration of the first nucleic acid molecule and the third concentration of the second nucleic acid molecule for preparation of the nucleic acid probe or preparing the nucleic acid probe with the first nucleic acid molecule at the first concentration and the second nucleic acid molecule at the third concentration.