Patent ID: 11891645
Assignee: NOVOZYMES A/S
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 29:
30. The variant of claim 27, comprising at least one substitution selected from the group consisting of: I2L, A17P, P22V, Q41L, Q41Y, F42R, Q54R, Q54S, Q54Y, F55N, F55T, K57L, K57V, I59D, I59S, I59V, S60L, S60Q, S60R, S60Y, S61F, S62L, S62V, T63A, T63E, T63R, T64C, T64D, T64M, Q112H, Q112M, Q112R, Q112S, G114A, G114C, G114L, G114P, N115Y, E129L, E129S, E129V, E129Y, N131I, N131S, N131V, Q134A, Q134L, Q134R, A149D, T167A, T167S, T167W, F199Y, A242E, K250R, V266A, V266F, V266L, V266S, V266T, V266Y, D267F, D267L, D267T, D267V, Q269C, Q269F, Q269I, Q269S, Q269T, Q269V, Q269X, I270A, I270C, I270L, I270S, V271F, V271I, V271K, V271M, S272A, G274R, G278S, T288A, T288C, T288G, V296P, V296R, V296T, F314L, S329D, S329V, S331A, S331G, S331T, P333V, S336G, S336L, and S336T, wherein the substitution at the one or more positions provides a protease variant having an increase in expression level measured as improvement factor, EIF, of at least 1.6.