Patent ID: 11873481
Assignee: ARIMA GENOMICS, INC.
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 0:
1. A method for preparing library nucleic acid templates, comprising:
reacting isolated nucleic acid with one or more first reagents that generate proximity ligated nucleic acid molecules; and
reacting the proximity ligated nucleic acid molecules with one or more second reagents that fragment and attach barcode oligonucleotides to the proximity ligated nucleic acid molecules thereby providing barcoded templates that are in virtual compartments, wherein the virtual compartments are not physically barred from intermixing with other virtual compartments, and wherein the barcode oligonucleotides attached to the barcoded templates in one of the virtual compartments are distinguishable from the barcode oligonucleotides attached to the barcoded templates in other virtual compartments and barcodes in the barcode oligonucleotides preserve molecular contiguity information for proximity ligated molecules.