Patent ID: 11926873
Assignee: THE TRANSLATIONAL GENOMICS RESEARCH INSTITUTE
Field: Biotechnology (Chemistry)
Classification: CPC C  G | IPC C

Claim 8:
9. A method of predicting onset of a migraine in a human subject, the method comprising the steps of:
obtaining a set of samples of biofluid from the subject collected over time intervals and stored on sample collection apparatuses, wherein the biofluid is blood;
extracting nucleic acids from the set of samples, wherein the nucleic acids are RNA;
sequencing the extracted nucleic acids to generate sequence data, wherein the RNA is sequenced with RNA-Seq;
analyzing the sequence data to identify changes in gene expression syntaxin binding protein 3 (STXBP3), wherein an at least five times increase in expression of STXBP3 compared to an earlier sample indicates onset of a migraine in the subject; and
treating the human subject for migraine when an at least five times increase in expression of STXBP3 compared with a sample collected at an earlier time interval occurs, wherein treating the migraine comprises administering to the subject an effective amount of a non-steroidal anti-inflammatory drug (NSAID), a triptan, an ergotamine, metoclopramide, lidocaine or a combination thereof;
wherein the sample is stored on a sample collection apparatus, the sample collection apparatus comprises cellulose paper on which the biofluid is placed to dry, and the cellulose paper has not been treated with any chemical stabilizers of nucleic acids.