Patent ID: 11859243
Assignee: SEEGENE, INC.
Field: Computer technology (Electrical engineering)
Classification: CPC C  G | IPC C  G

Claim 23:
24. A non-transitory computer readable storage medium containing instructions to configure a processor to perform a method for differentiating signals of interest for each of target nucleic acid sequences in the number of N in a sample, which are not differentiable by a single type of detector, the method comprising:
(a) receiving signals detected at detection temperatures in the number of N; wherein each of the target nucleic acid sequences in the sample is detected by a corresponding signal-generating means; wherein the signals of interest to be generated by the signal-generating means in the number of N are not differentiated for each target nucleic acid sequence by a single type of detector; wherein N is an integer not less than 2;
(b) providing the following equations in the number of N each of which comprises variables representing the signals of interest generated at each detection temperature for the target nucleic acid sequences;, S
       
        T
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      +
      
       S
       
        T
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        2
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      +
      …
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       S
       
        TND
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        1
       
      
     
     =
     
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       1
      
     
    
   
   
    
     (
     1
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         1
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       +
       
        S
        
         T
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         2
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         D
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         2
        
       
       +
       …
       +
       
        S
        
         TND
         ⁢
         2
        
       
      
      =
      
       S
       
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     ⁢
     

     ⋮
    
   
   
    
     (
     2
     )
    
   
  
 

 
  
   
    
     
      
       S
       
        T
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        1
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        ⁢
        N
       
      
      +
      
       S
       
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        ⁢
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        DN
       
      
      +
      …
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       S
       TNDN
      
     
     =
     
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      DN
     
    
   
   
    
     (
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wherein each of(SD1) to (SDN) is a signal detected at each detection temperature; each of(ST1D1) to (STNDN) is a variable representing a signal of interest generated by each signal-generating means at each detection temperature; and the total number of the variables is N2;

(c) providing additional equations in the number of (N2−N) selected from the group consisting of the following equations:

f(STjDα,STjDβ)=RVTj(DαDβ)  (X), and

STjDK=0  (XI)

wherein, j in Tj represents all integers starting from 1 to a Nth integer; for each jth target nucleic acid, α and β jointly represent a combination of two integers selected from 1 to N; wherein the number of the combination is represented by NC2; for each jth target nucleic acid, K is at least one of integers selected from 1 to N; RVTj(DαDβ) is a reference value (RV) of the jth target nucleic acid sequence (Tj) representing relationship reflecting change in signals provided by the jth signal-generating means at the αth detection temperature and the βth detection temperature; f(STjDα, STjDβ) represents a function of STjDα and STjDβ;
wherein STjDK=0 in the equation (XI) may be selected with a proviso that the jth signal-generating means is prepared to generate no signal in the presence of the jth target nucleic acid sequence at the Kth detection temperature
wherein f(STjDα, STjDβ)=RVTj(DαDβ) is STjDα or/STjDβ=RVTjDα/Dβ (XII) or STjDβ/STjDα=RVTjDβ/Dα (XIII); and
(d) obtaining solutions to at least one of the variables by the equations in the number of N2 provided in the steps (b) and (c) for differentiating at least one of the signals of interest to be assigned to at least one of the target nucleic acid sequences in the number of N.