Patent ID: 11964997
Assignee: REALSEQ BIOSCIENCES, INC.
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 2:
3. The method of claim 1, comprising ligating said plurality of combo adapters to the 3′ end of said plurality of sample polynucleotides wherein (a) said plurality of combo adapters comprises an adenylated 5′ end (5′-App) and a 3′-p, and wherein the 3′p is ligated to a 3′-OH end of a sample polynucleotide having a 5′-p or 5′-OH, or (b) said plurality of combo adapters comprising 5′-p and 3′-p ends is ligated to a 3′-OH end of a sample polynucleotide comprising a 5′-OH end.