Patent ID: 11920170
Assignee: DANISCO US INC.
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 0:
1. A recombinant variant of a parent α-amylase comprising:
a mutation at an amino acid residue corresponding to R377; and
at least one mutation at an amino acid residue, or residues, corresponding to an amino acid residue selected from the group consisting of N126, F153, T180, E187, and I203;
wherein the variant α-amylase or the parent α-amylase has at least 95%, amino acid sequence identity relative to SEQ ID NO: 1, which is used for numbering; and
wherein the variant has increased low pH stability and/or starch liquefaction activity, compared to the parent α-amylase or a reference α-amylase differing from the variant α-amylase only by the absence of the mutations.