Patent ID: 11969452
Assignee: MARCO RUGGIERO
Field: Pharmaceuticals (Chemistry)
Classification: CPC A | IPC A

Claim 0:
1. A process to manufacture a lyophilized probiotic powder composition aimed at protection from harmful effects of electromagnetic radiations, comprising the steps of:
providing a medium for the growth of probiotic microbes constituted by carbonated radioactive mineral water whose radioactivity is due to 226Radium at 152 mBq L−1 and 238Uranium at 217.7 mBq L−1, comprising: 40 g/L Equisetum arvense, 40 g/L white sugar, 15 ml/L lemon juice, and 10 ml/L apple cider vinegar;
inoculating the medium with Arthrospira platensis at 45 g/L and 10 mg/L of a probiotic mixture comprising: Lactobacillus paracasei, Lactobacillus casei, Lactobacillus helveticus, Lactobacillus kefiranofaciens, Lactobacillus plantarum, Lactobacillus gallinarum, Lactobacillus delbrueckii, Lactobacillus rhamnosus, Lactobacillus salivarium, Lactobacillus acidophilus, Lactobacillus farraginis, Lactobacillus antri, Lactococcus lactis, Lactococcus diacetylactis, Lactococcus cremoris, Bifidobacterium infantis, Bifidobacterium bifidum, Bifidobacterium lactis, and Bifidobacterium longum; 
cultivating Arthrospira platensis and the probiotic mixture in the medium comprising radioactive carbonated mineral water with Equisetum arvense at a temperature range of 36° C. to 39° C. for 48 h, with stirring at 0, 12, 24, and 36 h, to produce a probiotic composition;
harvesting the probiotic composition from the medium;
lyophilizing the probiotic composition to produce a lyophilized probiotic powder; and
encapsulating the lyophilized probiotic powder.