Patent ID: 11866753
Assignee: INBICON A/S
Field: Biotechnology (Chemistry)
Classification: CPC C  D  Y | IPC C  D

Claim 0:
1. A method of producing efficient C5 monomer yields from lignocellulosic biomass, said method comprising:
Pretreating a soft lignocellulosic biomass feedstock at pH within the range 3.5 to 9.0 in a single-stage pressurized hydrothermal pretreatment to produce a pretreated biomass having a xylan number of 10% or higher, wherein exogenous cellulase enzymes are not added during the pretreatment and said pretreating does not comprise adding sulphuric acid to the lignocellulosic feedstock,
Separating the pretreated biomass into a separated solid fraction and a separated liquid fraction, wherein the feedstock has a fiber structure that is maintained during pretreatment and separation,
Adding an enzyme mixture comprising endoglucanase, exoglucanase, B-glucosidase, endoxylanase, xylosidase and acetyl xylan esterase activities to the separated solid fraction to produce a hydrolysed solid fraction through enzymatic hydrolysis, wherein said enzyme mixture is not added to said separated liquid fraction, and
Subsequently mixing the separated liquid fraction and the hydrolysed solid fraction and conducting post-hydrolysis for a period of 6 to 50 hours, whereby at least 60% by mass of the xylo-oligomers in the separated liquid fraction are degraded to xylose monomers by the action of enzyme activities remaining within the hydrolysed solid fraction.