Patent ID: 11939385
Assignee: ALX ONCOLOGY INC.
Field: Pharmaceuticals (Chemistry)
Classification: CPC C  A | IPC A  C

Claim 0:
1. An activatable antibody comprising an antigen-binding domain (ABD), wherein the ABD comprises a heavy chain variable region (VH) and a light chain variable region (VL), wherein the N-terminus of the VH is fused to a first polypeptide shield moiety (S1), and the N-terminus of the VL is fused to a second polypeptide shield moiety (S2), wherein each of S1 and S2 is a polypeptide no more than about 50 amino acids long,
wherein association of S1 with S2 blocks binding of the ABD to its target,
wherein the ABD does not specifically bind to S1, S2, or association thereof,
wherein S1 comprises a first association moiety (B), and S2 comprises a second association moiety (C), wherein B and C are conjugated to each other by an isopeptide bond, and wherein S1 comprises the amino acid sequence of any one of SEQ ID NOs: 140, 142 and 227, and wherein S2 comprises the amino acid sequence of any one of SEQ ID NOs: 141 and 143; or wherein S1 comprises the amino acid sequence of any one of SEQ ID NOs: 141 and 143, and wherein S2 comprises the amino acid sequence of any one of SEQ ID NOs: 140, 142 and 227.