Patent ID: 11859251
Assignee: CHRONIX BIOMEDICAL
Field: Computer technology (Electrical engineering)
Classification: CPC C  G | IPC C  G

Claim 11:
12. A method of analyzing nucleic acid sequences, the method comprising:
(a) performing whole genome amplification on nucleic acids extracted from serum or plasma of a population of cancer patients;
(b) enzymatically cutting the whole genome amplification product of (a);
(c) ligating sequencing primers comprising identifiers to the enzymatically cleaved product of (b), wherein the resulting product consists of up to 20 sequence tags;
(d) sequencing the products of step (c), thereby generating at least 50,000 sequences per sample;
(e) receiving sequence information on a processor describing the sequenced nucleic acids of (d);
(f) receiving sequence information on said processor describing nucleic acid sequences from a cancer free control population;
(g) performing an analysis comparing the information received on the processor in steps (e) and (f) within a finite number of cluster regions of the human genome by aligning sequences within the finite number of cluster regions from said sequenced acellular nucleic acids from the population of cancer patients with one or more sequences from acellular nucleic acids obtained from the cancer free control population; and
(h) identifying one or more biomarkers in the serum or plasma of the cancer patient by the processor.