Patent ID: 11903383
Assignee: ASYMPTOTE LTD.
Field: Basic materials chemistry (Chemistry)
Classification: CPC A | IPC A

Claim 0:
1. A method for cryopreservation of an initially liquid sample comprising a biological material in a cryopreservation medium, the method comprising the step of cooling the top surface of the sample to selectively form an ice layer at the top surface of the sample, and further cooling the sample to provide formation of ice from said ice layer at the top surface progressively downwardly toward a base of the sample whereat the biological material is substantially located or concentrated; wherein the cooling applied the top surface of the sample is continued until a layer of the sample below the ice layer solidifies as a glass to form a vitrified, or amorphous solid, layer as a result of cooling below the glass transition temperature, wherein the cryopreservation medium is an aqueous solution comprising at least one cryoprotectant.