Patent ID: 11946050
Assignee: APTAH BIO, INC.
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 15:
16. A method for altering a pre-messenger ribonucleic acid (pre-mRNA) in a cell, the method comprising contacting said cell with the engineered polynucleotide of claim 1, wherein said first nucleotide sequence hybridizes with said pre-mRNA at said splice site, and said second nucleotide sequence hybridizes with said pre-mRNA at said sequence 5′ or 3′ to said splice site, and said recruiting nucleotide sequence hybridizes to the SL2 of said U1 snRNA and/or binds to said U1-C zinc finger, wherein said U1 snRNA and the U1-C zinc finger are components of a spliceosome, to splice said pre-mRNA in said cell, thereby altering the pre-mRNA.