Patent ID: 11865196
Assignee: SHANGHAI UNIVERSITY
Field: Pharmaceuticals (Chemistry)
Classification: CPC A  C | IPC A  C

Claim 2:
3. A method for preparing an engineered radioactive polymeric microsphere, comprising:
adding 12 g of a styrene monomer and 4 g of sodium dodecyl sulfate (SDS) to 80 mL of deionized water, followed by stirring at room temperature in a nitrogen atmosphere for 1 h to obtain a first reaction mixture;
heating the first reaction mixture to 50° C. and adding 1.2 g of azobisisobutyronitrile to the first reaction mixture, followed by reaction under stirring at a constant temperature for 3 h to obtain a second reaction mixture; and
subjecting the second reaction mixture to washing with ethanol and 60° C. water, and vacuum drying to obtain a crude polymeric microsphere;
weighing 4 g of the crude polymeric microsphere followed by irradiation in the exposure to an electron beam at room temperature under an air atmosphere to obtain an irradiated polymeric microsphere, wherein a radiation absorbed dose is 200 kGy; adding 50 mL of deionized water, 10 mL of acrylic acid and 10 g of 2-methacryloyloxyethyl phosphorylcholine to a conical flask followed by stirring for 2 h to obtain a mixed solution; adding 2 g of the irradiated polymeric microsphere to the mixed solution followed by nitrogen feeding for 30 min to remove oxygen and sealing to obtain a third reaction mixture; subjecting the third reaction mixture to reaction in a water bath for 5 h and vacuum filtration to obtain a grafted polymeric microsphere; and subjecting the grafted polymeric microsphere to washing with deionized water and drying in a vacuum oven for 24 h to obtain a carboxyl-phosphorylcholine-based polymeric microsphere; and
mixing 1.2 g of the carboxyl-phosphorylcholine-based polymeric microsphere with 2 mL of a 0.5 mCi/μL 177LuCl3 solution at room temperature under ultrasonic oscillation for 30 min to obtain an engineered carboxyl-phosphorylcholine-based 177Lu polymeric microsphere with a diameter of 60 μm.