Patent ID: 11884700
Assignee: ICHNOS SCIENCES SA
Field: Pharmaceuticals (Chemistry)
Classification: CPC C  A | IPC A  C

Claim 8:
9. A method for the removal of impurities from a clarified harvest antibody material, the method comprising: (a) culturing host cells which express an antibody, (b) harvesting the antibody material in the cell culture and (c) subjecting it to a clarification step, wherein said method further comprises (d) subjecting said clarified harvest antibody material to protein A chromatography, (e) treating a resulting protein A eluate with high pH to reach a high target pH and (f) subjecting the high pH treated protein A eluate to a filtration step to remove impurities comprising host cell proteins, nucleic acids and/or cell debris, wherein said high target pH is about 10.2 to about 11.0.