Patent ID: 11866476
Assignee: XILIO DEVELOPMENT, INC.
Field: Pharmaceuticals (Chemistry)
Classification: CPC C  A | IPC A  C

Claim 0:
1. A masked cytokine comprising:
a) a first polypeptide comprising a first half-life extension domain and a masking moiety and
b) a second polypeptide comprising a second half-life extension domain and a cytokine;
wherein the first half-life extension domain is a first Fc domain or fragment thereof, and the second half-life extension domain is a second Fc domain or fragment thereof;
wherein the masking moiety comprises an amino acid sequence of SEQ ID NO: 826
wherein the cytokine is an IL-2 polypeptide comprising the amino acid sequence of SEQ ID NO: 3;
wherein the masking moiety is linked to the C-terminus of the first half-life extension domain via a first linker;
wherein the cytokine is linked to the C-terminus of the second half-life extension domain via a second linker, wherein the second linker comprises a cleavable peptide; and
wherein the first half-life extension domain and the second half-life extension domain contain modifications promoting the association of the first and the second half-life extension domain.