Patent ID: 11935629
Assignee: BECTON, DICKINSON AND COMPANY
Field: Measurement (Instruments)
Classification: CPC G | IPC G

Claim 0:
1. A method for identifying a set of fluorophore-biomolecule reagent pairs for characterizing a sample in a flow cytometer, the method comprising:
calculating a spectral spillover spreading parameter for a plurality of fluorophores, wherein calculating the spectral spillover spreading parameter for each of the plurality of fluorophores comprises generating a matrix of fluorescence spillover spreading values for the plurality of fluorophores;
calculating a sum of each row of the generated matrix wherein the sum of each row of the generated matrix comprises an approximation of the spectral spillover spread by each individual fluorophore into the plurality of fluorophores;
calculating a sum of each column of the generated matrix wherein the sum of each column of the generated matrix comprises an approximation of the spectral spillover spread by the plurality of fluorophores into each individual fluorophore;
pairing each fluorophore with a biomolecule that is specific for a biomarker of a cell in the sample to generate a plurality of fluorophore-biomolecule reagent pairs;
generating an adjusted spillover spreading matrix for the fluorophore-biomolecule reagent pairs based on the spectral spillover spreading parameter of each fluorophore and a biomarker classification parameter; and
identifying an optimal set of fluorophore-biomolecule reagent pairs based on the calculated spillover spreading values from the adjusted spillover spreading matrix;
characterizing cells of the sample with the flow cytometer using the identified set of fluorophore-biomolecule reagent pairs.