Patent ID: 11859231
Assignee: MEDTRONIC MINIMED, INC.
Field: Measurement (Instruments)
Classification: CPC C  A  G | IPC A  C  G

Claim 15:
16. A method of sensing glucose concentrations in a fluid comprising:
(a) disposing an electrochemical glucose sensor in the fluid, wherein the electrochemical glucose sensor comprises:
a base layer;
a working electrode disposed on the base layer; and
a multilayer analyte sensor stack comprising:
(i) an analyte sensing layer comprising glucose oxidase disposed over the working electrode, wherein the analyte sensing layer detectably alters the electrical current at the working electrode in the presence of an analyte;
(ii) a high-density amine layer comprising poly-l-lysine polymers, wherein the high-density amine layer is disposed over the analyte sensing layer; and

(d) an analyte modulating layer disposed over the high-density amine layer, wherein:
the analyte modulating layer modulates the diffusion of glucose therethrough; and
the high-density amine layer comprises a first side in direct contact with the analyte sensing layer or the analyte modulating layer;

(b) monitoring fluctuations in electrical conductivity; and
(c) correlating the fluctuations in electrical conductivity with a concentration of glucose;, so that glucose concentrations in the fluid are sensed.