Patent ID: 11932899
Assignee: 10X GENOMICS, INC.
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 13:
14. A method for determining an epigenetic characteristic of a nucleic acid molecule from a cell, comprising:
(a) providing a cell bead comprising the cell, the cell comprising the nucleic acid molecule from the cell, wherein the nucleic acid molecule contains or is suspected of containing one or more hydroxymethylated bases;
(b) partitioning the cell bead with a nucleic acid barcode molecule comprising a cell barcode sequence in a partition among a plurality of partitions;
(c) in the partition, using the nucleic acid molecule and the nucleic acid barcode molecule to synthesize a barcoded nucleic acid molecule, which barcoded nucleic acid molecule comprises the cell barcode sequence, wherein the cell barcode sequence is indicative of the cell from which the nucleic acid molecule originates;
(d) processing the barcoded nucleic acid molecule or a derivative thereof to identify the one or more hydroxymethylated bases as hydroxymethylated and the cell barcode sequence; and
(e) using the one or more hydroxymethylated bases and the cell barcode sequence to determine the epigenetic characteristic of the nucleic acid molecule as being associated with the cell.