Patent ID: 11952580
Assignee: TEKNOLOGIAN TUTKIMUSKESKUS VTT OY
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 4:
5. A method for producing metabolites comprising:
a. providing a recombinant plant host cell comprising:
heterologous polynucleotides encoding PsiD, PsiH, PsiK, and PsiM;
wherein the heterologous polynucleotides are operably linked to at least one promoter which is capable of directing expression of said heterologous polynucleotides in the recombinant plant host cell;

wherein PsiD has at least 80% amino acid sequence identity with the protein sequence deposited in GenBank accession number ASU62239.1 or the GenBank accession number ASU62242.1, or with the amino acid sequence encoded by polynucleotide SEQ ID NO: 1 or 2 or 9 or 10;
wherein PsiH has at least 80% amino acid sequence identity with the protein sequence deposited in GenBank accession number ASU62246.1 or the GenBank accession number ASU62250.1, or with the amino acid sequence encoded by polynucleotide SEQ ID NO: 5 or 6 or 13 or 14;
wherein PsiK has at least 80% amino acid sequence identity with the protein sequence deposited in GenBank accession number ASU62237.1 or the GenBank accession number ASU62240.1, or with the amino acid sequence encoded by polynucleotide SEQ ID NO: 7 or 8 or 15 or 16;
wherein PsiM has at least 80% amino acid sequence identity with the protein sequence deposited in GenBank accession number ASU62238.1 or the GenBank accession number ASU62241.1, or with the amino acid sequence encoded by polynucleotide SEQ ID NO: 3 or 4 or 11 or 12; and
wherein the recombinant plant host cell is capable of producing psilocybin;

b. cultivating the recombinant plant host cell in conditions allowing growth and propagation of the recombinant plant host cell;
c. continuing cultivating the recombinant plant host cells to synthesize the metabolites; and
d. recovering at least one metabolite synthesised by an enzyme encoded by the heterologous polynucleotide of the recombinant plant host cell.