Patent ID: 11958882
Assignee: AMGEN INC.
Field: Measurement (Instruments)
Classification: CPC C  A  B  G | IPC A  B  C  G

Claim 3:
4. The method of claim 1, wherein (A) the number of volumes of the first solution per chamber is 2 or more during step (i), (B) FR1 is about 50 ml/min to about 150 ml/min during step (i), (C) the first solution is a hypertonic crystallization buffer, (D) the second solution is an isotonic formulation buffer, (E) the second solution is an isotonic formulation buffer, (F) the Fcentrifugal is 1000 g during step (ii), (F) the feed volume of the second stream of the second solution is about 100 to about 500 ml during step (ii), (G) the number of volumes of the first solution per chamber is 1 or 2 during step (ii), (H) FR2 is about 10 ml/min to about 50 ml/min during step (ii), (I) the concentration of the crystalline biomolecules is increased at least 2-fold, (J) the method comprises decreasing Fcentrifugal to a level of about 5 g and about 20 g, (K) FR1 is controlled by a first pump and the first pump is set to about 10 ml/min to about 100 ml/min after step (b), (L) the rotating chamber is connected to a chamber peristaltic pump, (M) the method is carried out in an apparatus comprising more than one rotating chamber, (N) the crystalline biomolecule is a protein or comprises one or more polypeptide chains, or (O) any combination thereof.