Patent ID: 11958913
Assignee: BIOMUNEX PHARMACEUTICALS
Field: Biotechnology (Chemistry)
Classification: CPC C  A | IPC A  C

Claim 0:
1. A multispecific antigen-binding fragment comprising at least two Fab fragments, wherein each Fab fragment recognizes a different epitope of interest, and said Fab fragments are tandemly arranged in any order, the C-terminal end of the CH1 domain of a first Fab fragment being linked to the N-terminal end of the VH domain of the following Fab fragment through a polypeptide linker,
characterized in that the polypeptide linker sequence comprises EPKX1CDKX2HX3X4PPX5PAPELLGGPX6X7PPX8PX9PX10GG (SEQ ID NO: 1),
wherein:
X1, X2 and X3, identical or different, are Threonine (T) or Serine (S);
X4 and X5, identical or different, are any amino acid selected from the group consisting of Serine (S), Cysteine (C), Alanine (A), and Glycine (G); and
X6, X7, X8, X9, X10, identical or different, are selected from the group consisting of Alanine (A), Glycine (G), Valine (V), Asparagine (N), Aspartic acid (D) and Isoleucine (I).