Patent ID: 11919936
Assignee: SHANGRAO CONCORD PHARMACEUTICAL CO., LTD.
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 19:
20. A method for preparing a polypeptide of interest, which comprises:
(1) culturing cells for expressing a fusion protein comprising the polypeptide of interest and the TEV protease variant according to claim 3 that is fused to the polypeptide of interest, in a medium under suitable conditions for culturing the cells;
(2) obtaining inclusion bodies of the fusion protein;
(3) dissolving the inclusion bodies under about 8 M urea or about 6 M guanidine hydrochloride;
(4) incubating under the condition of 3 M-5 M urea or 1 M-2 M guanidine hydrochloride at for 10-24 hours;
(5) precipitating the TEV protease variant after dilution with buffer;
(6) removing precipitate of the TEV protease variant to obtain the polypeptide of interest; and
(7) purifying the polypeptide of interest.