Patent ID: 11965882
Assignee: CELL.COPEDIA GMBH
Field: Measurement (Instruments)
Classification: CPC G  C | IPC C  G

Claim 2:
3. The method of claim 1, wherein the first to third ligand binding partners (7) are a streptavidin mutein comprising the amino acid sequence Val44-Thr45-Ala46-Arg47 represented by SEQ ID NO: 13 or Ile44-Gly45-Ala46-Arg47 represented by SEQ ID NO: 14, and the ligand (5) is a streptavidin-binding peptide comprising one of the following sequences:
a) -Trp-Xaa-His-Pro-Gln-Phe-Yaa-Zaa- represented by SEQ ID NO: 1, wherein Xaa is any amino acid and Yaa and Zaa are both Gly or Yaa is Glu and Zaa is Lys or Arg,
b) -Trp-Arg-His-Pro-Gln-Phe-Gly-Gly- represented by SEQ ID NO: 2,
c) -Trp-Ser-His-Pro-Gln-Phe-Glu-Lys- represented by SEQ ID NO: 3,
d) a sequential arrangement of at least two streptavidin binding peptides, wherein each peptide binds streptavidin, wherein the distance between two peptides is at least 0 and not greater than 50 amino acids and wherein each of the at least two peptides comprises the amino acid sequence -His-Pro-Baa- in which Baa is selected from the group consisting of glutamine, asparagine and methionine, and wherein the sequential arrangement comprises at least the amino acid sequence -His-Pro-Baa-Xaa-His-Pro-Baa- (SEQ ID NO: 21), in which Xaa can be 0-50 amino acids of any type, and in which Baa is selected from the group consisting of glutamine, asparagine and methionine,
e) a sequential arrangement as recited in d), wherein one of the at least two peptides comprises the sequence -His-Pro-Gln-, and wherein the sequential arrangement comprises at least the amino acid sequence -His-Pro-Gln-Xaa-His-Pro-Gln- (SEQ ID NO: 22), in which Xaa can be 0-50 amino acids of any type,
f) a sequential arrangement as recited in d), wherein one of the peptides comprises an amino acid sequence -His-Pro-Gln-Phe- represented by SEQ ID NO: 4, and wherein the sequential arrangement comprises at least the amino acid sequence -His-Pro-Gln-Phe-Xaa-His-Pro-Gln-Phe-(SEQ ID NO: 23), in which Xaa can be 0-50 amino acids of any type,
g) a sequential arrangement as recited in d) wherein at least one peptide includes at least the amino sequence -Oaa-Xaa-His-Pro-Gln-Phe-Yaa-Zaa- represented by SEQ ID NO: 5, wherein Oaa is Trp, Lys or Arg, Xaa is any amino acid and wherein either Yaa and Zaa are both Gly or Yaa is Glu and Zaa is Lys or Arg, and wherein the sequential arrangement comprises at least the amino acid sequence -Oaa-Xaa-His-Pro-Gln-Phe-Yaa-Zaa-Xaa-Oaa-Xaa-His-Pro-Gln-Phe-Yaa-Zaa-(SEQ ID NO: 24), in which Xaa at position 9 can be 0-50 amino acids of any type, where Oaa is Trp, Lys or Arg, Xaa at position 2 and 11 is any amino acid, and where either Yaa and Zaa are both Gly or Yaa is Glu and Zaa is Lys or Arg,
h) a sequential arrangement as recited in d) wherein at least one peptide includes at least the amino acid sequence -Trp-Xaa-His-Pro-Gln-Phe-Yaa-Zaa- represented by SEQ ID NO: 6 wherein Xaa is any amino acid and wherein either Yaa and Zaa are both Gly or Yaa is Glu and Zaa is Lys or Arg, and wherein the sequential arrangement comprises at least the amino acid sequence -Trp-Xaa-His-Pro-Gln-Phe-Yaa-Zaa-Xaa-Trp-Xaa-His-Pro-Gln-Phe-Yaa-Zaa-(SEQ ID NO: 25), in which Xaa at position 9 can be 0-50 amino acids of any type, where Xaa at position 2 and 11 is any amino acid, and where either Yaa and Zaa are both Gly or Yaa is Glu and Zaa is Lys or Arg,
i) a sequential arrangement as recited in d) wherein at least one peptide includes at least the amino acid sequence -Trp-Ser-His-Pro-Gln-Phe-Glu-Lys- represented by SEQ ID NO: 7, and wherein the sequential arrangement comprises at least the amino acid sequence -Trp-Ser-His-Pro-Gln-Phe-Glu-Lys-Xaa-Trp-Ser-His-Pro-Gln-Phe-Glu-Lys-(SEQ ID NO: 26), in which Xaa can be 0-50 amino acids of any type,
j) the amino acid sequence -Trp-Ser-His-Pro-Gln-Phe-Glu-Lys-(Xaa)n-Trp-Ser-His-Pro-Gln-Phe-Glu-Lys-represented by SEQ ID NO: 8 wherein Xaa is any amino acid and n is an integer from 0 to 12,
k) an amino acid sequence selected from the group consisting of Trp-Arg-His-Pro-Gln-Phe-Gly-Gly represented by SEQ ID NO: 2, Trp-Ser-His-Pro-Gln-Phe-Glu-Lys represented by SEQ ID NO: 9, Trp-Ser-His-Pro-Gln-Phe-Glu-Lys-(GlyGlyGlySer)3-Trp-Ser-His-Pro-Gln-Phe-Glu-Lys represented by SEQ ID NO: 10, Trp-Ser-His-Pro-Gln-Phe-Glu-Lys-(GlyGlyGlySer)2-Trp-Ser-His-Pro-Gln-Phe-Glu-Lys represented by SEQ ID NO: 11 and Trp-Ser-His-Pro-Gln-Phe-Glu-Lys-(GlyGlyGlySer)2-Gly-Gly-Ser-Ala-Trp-Ser-His-Pro-Gln-Phe-Glu-Lys represented by SEQ ID NO: 12.