Patent ID: 11958051
Assignee: TECAN TRADING AG
Field: Measurement (Instruments)
Classification: CPC B  G  C | IPC B  G

Claim 0:
1. Method for reducing liquid evaporation from wells of a microplate (10), which method comprises:
a) providing the microplate (10) having a plurality of wells,
b) adding a sample to at least one of the wells of the microplate (10),
c) pushing the microplate (10) or an incubation cassette (1) equipped with the microplate (10) into a microplate reader (3),
d) injecting a liquid (F) into a liquid reservoir (9) which is provided in the microplate (10) and/or in the incubation cassette (1),
e) carrying out measurements on the samples in the respective wells,
f) optically measuring a liquid level in the liquid reservoir (9) of the microplate (10) and/or of the incubation cassette (1) through a substantially transparent portion (TA) of the liquid reservoir (9) of the microplate (10) and/or the liquid reservoir (9) of the incubation cassette (1),
g) re-injecting the liquid (F) into the liquid reservoir (9) of the microplate (10) and/or into the liquid reservoir (9) of the incubation cassette (1) in response to determining that the liquid level is below a predetermined threshold value,
h) repeating steps e) to g) until a predetermined number of measurement cycles is reached,
i) pulling the microplate (10) or the incubation cassette (1) equipped with the microplate (10) out of the microplate reader (3),
wherein the liquid reservoir (9) is provided between the wells and is bounded by a wall of the microplate (10) and is formed to hold a liquid (F),
wherein the incubation cassette (1) comprises a frame (4) for receiving a microplate (10) having wells, wherein the frame (4) comprises a central first opening (5) which is surrounded by an inner wall (6), the dimensions of which are designed for the placement of a microplate (10) therein, and wherein the frame (4) comprises an outer wall (8) which extends substantially parallel to the inner wall (6) and which adjoins the inner wall (6) via an intermediate bottom (14) such that a liquid reservoir (9) for holding a liquid (F) is formed by the two walls (6, 8) and the intermediate bottom (14), said liquid reservoir surrounding the first central opening (5), wherein at least a portion (6, 8, 14) of the incubation cassette (1) that forms the liquid reservoir (9) is provided at least in part with said substantially transparent portion (TA).