Patent ID: 11872256
Assignee: SYNTBIOLAB INC.
Field: Pharmaceuticals (Chemistry)
Classification: CPC A  C  Y | IPC A  C

Claim 0:
1. A method for manufacturing a bacteriophage cocktail comprising at least two bacteriophage strains for the prevention of an opportunistic bacterial infection caused by pathogenic bacterial cells in livestock, the method comprising:
a) characterizing at least two bacteriophage strains based on:
(i) an absence of a gene encoding an integrase;
(ii) an absence of a 16s rRNA gene marker;
(iii) an absence of a gene encoding a human toxin;
(iv) an absence of an antibiotic resistance gene;
(v) a burst size greater than 10 against said pathogenic bacterial cells;
(vi) stability at pH 4;
(vii) stability at a temperature between 4° C. and room temperature (RT); and
(viii) an absence of cross-resistance by said pathogenic bacterial cells against at least two bacteriophage strains by:
providing a first and a second bacteriophage strain that infect the Pathogenic bacteria, the second bacteriophage strain being different from the first bacteriophage strain;
culturing the first bacteriophage strain in the presence of said pathogenic bacteria at a Multiplicity of Infection (MOI) of at least about 1 until said bacteria develop resistance to the first bacteriophage strain, thereby producing a Bacteriophage Insensitive Mutant (BIM) pathogenic bacterial strain;
challenging the BIM pathogenic bacterial strain with the second bacteriophage strain; and
determining the absence of cross-resistance when the second bacteriophage strain still inhibits the growth of the BIM pathogenic bacterial strain; and

b) selecting at least two bacteriophage strains characterized based on each of (i)-(viii) in step a), and combining to form the cocktail.