Patent ID: 11965191
Assignee: CALIFORNIA INSTITUTE OF TECHNOLOGY
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 12:
13. The synthetic protein circuit of claim 1, further comprising a second target protein comprising a first degron of the second target protein that destabilizes the second target protein when present on the second target protein;
wherein the first target protein further comprises a first cut site specific for the first protease between the first degron of the first target protein and a distal part of the first target protein from the first degron the first target protein, a second degron of the first target protein, and a second cut site specific for the second protease connected to the second degron of the first target protein, wherein the first target protein is destabilized by the first degron of the first target protein unless first cut site specific for the first protease is cleaved by the first protease, and wherein the first target protein is destabilized by cleavage of the second cut site specific for the second protease; and
wherein the second target protein further comprises a first cut site specific for the second protease between the first degron of the second target protein and a distal part of the second target protein from the first degron the second target protein, a second degron of the second target protein, and a second cut site specific for the first protease connected to the second degron of the second target protein, wherein the second target protein is destabilized by the first degron of the second target protein unless first cut site specific for the second protease is cleaved by the second protease, and wherein the second target protein is destabilized by cleavage of the second cut site specific for the first protease.