Patent ID: 11926840
Assignee: NATIONAL INSTITUTE OF ADVANCED INDUSTRIAL SCIENCE AND TECHNOLOGY
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 14:
15. A method for reconstituting a stealth RNA gene expression system, comprising:
(1) preparing an Escherichia coli expressing T7 RNA polymerase;
(2) introducing into the Escherichia coli host of the (1), at least a vector for Escherichia coli carrying an RNA encoding an RNA-dependent RNA polymerase and an RNA binding protein, and a vector for Escherichia coli for expressing a DNA encoding RNA binding protein, together with the negative-sense single-stranded RNA (A) according to claim 1 to transform the host,
(3) forming a complex of the negative-sense single-stranded RNA comprising exogenous gene RNA expressed by T7 RNA polymerase, and RNA binding protein in the transformed Escherichia coli of the (2),
(4) preparing animal cells in which an RNA-dependent RNA polymerase is expressed, and (5) introducing the complex of the negative-sense single-stranded RNA and the RNA binding protein obtained in the (3) into an animal cell host of the (4) to reconstitute a stealth RNA gene expression system comprising the negative-sense single-stranded RNA, and the complex of the RNA binding protein and the RNA-dependent RNA polymerase.