Patent ID: 11884946
Assignee: UNIVERSITY OF MASSACHUSETTS
Field: Medical technology (Instruments)
Classification: CPC C  A | IPC A  C

Claim 0:
1. A method of making a protein film on a substrate, the method comprising:
depositing a protein solution comprising one or more proteins on a substrate, to form the protein film on the substrate, wherein the one or more proteins are a fibrous protein, a globular protein, a transmembrane transport protein, a hormone or growth factor protein, a receptor protein, a DNA-binding protein, a transcription regulation protein, an immune system protein, a nutrient storage or transport protein, a chaperone protein, an enzyme, or a combination thereof;
immersing the protein film on the substrate in at least one organofluorine compound in liquid form that is chemically inert to the one or more proteins, and heating the at least one organofluorine compound to stabilize the protein film, wherein the stabilized protein film on the substrate retains the secondary structure of the one or more proteins in the protein solution;
removing the protein film on the substrate from immersion in the at least one organofluorine compound; and
washing the protein film on the substrate to remove any residual at least one organofluorine compound present following removal,
wherein the protein film on the substrate following removal from the immersion is a hydrophilic protein film and is free of any organofluorine compound and the organofluorine compound is optionally preheated ahead of immersion.