Patent ID: 11959100
Assignee: TAKEDA PHARMACEUTICAL COMPANY LIMITED
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 13:
14. A method for producing nerve cells, glial cells, mesenchymal stromal cells, bone cells, chondrocytes, corneal cells or pigment cells, comprising the steps of:
(i) suspension-culturing neural crest cells in a medium comprising a GSK3β inhibitor and a basic fibroblast growth factor, wherein the medium comprises the GSK3β inhibitor with a concentration that exhibits an effect equivalent to that exhibited by CHIR99021 with a concentration 3.0 μM to 4.5 μM higher than 1 μM and lower that 5 μM, thereby maintaining multipotent neural crest cells over 63 days or longer, wherein the cultured neural crest cells comprise a ratio of at least 75% or more of neural crest cells that maintain multipotency to cultured cell population; and
(ii) differentiating the neural crest cells obtained in the step (i) into cells of at least one lineage selected from the group consisting of nerve cells, glial cells, mesenchymal stromal cells, bone cells, chondrocytes, corneal cells and pigment cells.