Patent ID: 11919006
Assignee: INDIAN INSTITUTE OF TECHNOLOGY, KHARAGPUR
Field: Measurement (Instruments)
Classification: CPC B  G  C | IPC B  C  G

Claim 1:
2. A method as claimed in claim 1 wherein said controlled piecewise isothermal heating and cooling cycles are selected based on the pathogenic RNA, said RT LAMP reaction mixture including said RT LAMP reaction specific primers and said double modified DNA oligonucleotide probes along with said biotin labelled Forward Inner primer (FIP) and wherein all the primers used are designed to be non-AT rich with melting temperatures ranging in between 55° C. to 65° C. length of the amplicons within 200 base pairs and distance between the F2 to F1 and B2 to B1 maintained within 40-60 base pairs.