Patent ID: 11913080
Assignee: THE UNIVERSITY OF NORTH CAROLINA AT CHAPEL HILL
Field: Biotechnology (Chemistry)
Classification: CPC C  Y | IPC C

Claim 0:
1. A composition for detecting tumor-derived Human Papilloma Virus type 18 (HPV18) in a sample from a subject, comprising at least a triad of modified oligonucleotide primer/probe sets selected from the group consisting of:
Set 1: SEQ ID NO:80, SEQ ID NO:81, and SEQ ID NO:82,
Set 2: SEQ ID NO:83, SEQ ID NO:84, and SEQ ID NO:85,
Set 3: SEQ ID NO:86, SEQ ID NO:87, and SEQ ID NO:88,
Set 4: SEQ ID NO:89, SEQ ID NO:90, and SEQ ID NO:91,
Set 5: SEQ ID NO:92, SEQ ID NO:93, and SEQ ID NO:94,
Set 6: SEQ ID NO:95, SEQ ID NO:96, and SEQ ID NO:97,
Set 7: SEQ ID NO:98, SEQ ID NO:99, and SEQ ID NO:100,
Set 8: SEQ ID NO:101, SEQ ID NO:102, and SEQ ID NO:103,
Set 9: SEQ ID NO:104, SEQ ID NO:105, and SEQ ID NO:106,
Set 10: SEQ ID NO:107, SEQ ID NO:108, and SEQ ID NO:109,
Set 11: SEQ ID NO:110, SEQ ID NO:111, and SEQ ID NO:112,
Set 12: SEQ ID NO:113, SEQ ID NO:114, and SEQ ID NO:115,
Set 13: SEQ ID NO:116, SEQ ID NO:117, and SEQ ID NO:118,
Set 14: SEQ ID NO:119, SEQ ID NO:120, and SEQ ID NO:121,
Set 15: SEQ ID NO:122, SEQ ID NO:123, and SEQ ID NO:124,
Set 16: SEQ ID NO:125, SEQ ID NO:126, and SEQ ID NO:127,
Set 17: SEQ ID NO:128, SEQ ID NO:129, and SEQ ID NO:130, and
Set 18: SEQ ID NO:131, SEQ ID NO:132, and SEQ ID NO:133,
wherein a first primer/probe set of the triad is configured to produce a first amplicon signal,
wherein a second primer/probe set of the triad is configured to produce a second amplicon signal,
wherein a third primer/probe set of the triad is configured to produce a third amplicon signal, and
wherein the primer/probe set of the triad with the smallest set number corresponds to the first primer/probe set and the primer/probe set of the triad with the largest set number corresponds to the third primer/probe set.