Patent ID: 11926840
Assignee: NATIONAL INSTITUTE OF ADVANCED INDUSTRIAL SCIENCE AND TECHNOLOGY
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 15:
16. A stealth RNA which is a negative-sense single-stranded RNA (A), comprising:
(1) RNA sequences encoding any given protein or functional RNA,
(2) RNA sequences constituting noncoding region(s) that are unrecognizable by an innate immune system,
(3) transcription start signal sequences recognized by an RNA-dependent RNA polymerase,
(4) transcription termination signal sequences recognized by the RNA-dependent RNA polymerase,
(5) RNA sequences comprising replication origins recognized by the RNA-dependent RNA polymerase,
(6) RNA sequences encoding the RNA-dependent RNA polymerase and having a structure optimized to be unrecognizable by an innate immune system,
(7) an RNA sequence encoding a protein that regulates activity of the RNA-dependent RNA polymerase, and having a structure optimized to be unrecognizable by an innate immune system, and
(8) an RNA sequence encoding a single-stranded RNA binding protein and having a structure optimized to be unrecognizable by an innate immune system,
wherein the stealth RNA is capable of forming a complex that does not activate an innate immune system together with the single-stranded RNA binding protein (B), and the RNA-dependent RNA polymerase (C),
wherein the RNA-dependent RNA polymerase encoded by the RNA sequences of the (6) consists of L protein and P protein of an RNA virus belonging to a paramyxovirus family,
wherein the protein that regulates activity of the RNA-dependent RNA polymerase encoded by the RNA sequence of the (7) is C protein of the RNA virus belonging to the paramyxovirus family,
wherein the single-stranded RNA binding protein encoded by the RNA sequence of the (8) is NP protein of the RNA virus belonging to the paramyxovirus family,
wherein the RNA sequences of the (2) are RNA sequences having a length of 5 nucleotides to 49 nucleotides, and are a partial mRNA(s) of an animal gene expressed in animal cells, and
wherein all of the RNA sequences of the (3) to (5) are RNA sequences comprising a transcription start signal, a transcription termination signal, or a replication origin sequence isolated from a genome of the RNA virus belonging to the paramyxovirus family.