Patent ID: 11959117
Assignee: NATURE'S TOOLBOX, INC.
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 20:
21. A system for the production of pseudouridine compounds comprising
an isolated uracil nucleobase, and isolated ribose-5-phosphate, wherein said uracil nucleobase with a ribose-5-phosphate are converted to form pseudouridine-5′-monophosphate (ΨMP), wherein the formation of ΨMP is catalyzed by pseudouridine-5′-phosphate glycosidase or a functional fragment or variant thereof;
a protecting agent that reacts with the hydroxyl groups of said ΨMP forming a protected ΨMP; and
a methylating agent that reacts with the protected ΨMP, forming a protected N1-methyl-pseudouridine-5′-monophosphate (protected m1ΨMP) compound.