Patent ID: 11953440
Assignee: INL—INTERNATIONAL IBERIAN NANOTECHNOLOGY LABORATORY
Field: Measurement (Instruments)
Classification: CPC G | IPC G

Claim 0:
1. A method for simultaneous multicolor nonlinear imaging of multiple chromophores of different types in a sample, the method comprising:
a) emitting ultrashort broadband light pulses from a few-cycle laser system for excitation of the multiple chromophores;
b) focusing the ultrashort broadband light pulses in a sample plane of the sample;
c) controlling the focused ultrashort broadband light pulses temporal duration in the sample plane;
d) preventing the ultrashort broadband light pulses from directly reaching a detection system;
e) measuring nonlinear signals generated in the sample plane;
f) scanning the excitation and measurement on individual points (1D), or point-by-point in the sample plane (2D), or point-by-point across focal planes (3D);
g) synchronizing the measurement of the non-linear signals generated in the sample plane to a detector in order to acquire time resolved nonlinear signal information on photon arrival time histograms from the sample plane;
h) processing the time resolved nonlinear signal information;
i) analyzing the time resolved non-linear signal information for determination of decay parameters associated to the photon arrival time histograms in the individual points (1D), or point-by-point in the sample plane (2D), or point-by-point across focal planes (3D).