Patent ID: 11926873
Assignee: THE TRANSLATIONAL GENOMICS RESEARCH INSTITUTE
Field: Biotechnology (Chemistry)
Classification: CPC C  G | IPC C

Claim 0:
1. A method of predicting onset of a migraine in a human subject, the method comprising the steps of:
obtaining a set of samples of biofluid from the human subject collected over time intervals and stored on sample collection apparatuses, wherein the biofluid is blood and the sample collection apparatus comprises a cellulose paper on which the biofluid is placed to dry, and the cellulose paper has not been treated with any chemical stabilizers of nucleic acids;
extracting nucleic acids from the set of samples, wherein the nucleic acids are RNA;
sequencing the extracted nucleic acids to generate sequence data, wherein the RNA is sequenced with RNA-seq; and
analyzing the sequence data to identify increases in syntaxin binding protein 3 (STXBP3), wherein an at least five times increase in expression of STXBP3 compared with a sample collected at an earlier time interval indicates onset of a migraine in the human subject.