Patent ID: 11959145
Assignee: JOHANN BAUER
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 2:
3. A method for identifying a compound that ameliorates or reverts a defective or undesired translational read-through of a target gene bearing a premature termination codon (PTC) in a eukaryotic cell, said method comprising:
a) contacting a screening system according to claim 1 with a compound that potentially ameliorates or reverts the defective or undesired translational read-through of a target gene bearing a premature termination codon (PTC),
b) measuring the expression of a first reporter group in the presence and absence of said compound, and
c) measuring the expression of a second reporter group in the presence and absence of said compound as an internal standard;
wherein a compound that ameliorates or reverts a defective or undesired translational read-through of said PTC-bearing target gene is identified by
i) a change in the expression of said first reporter group in the presence of said compound compared to the expression of said first reporter group in the absence of said compound and
ii) the absence of a change in the expression of said second reporter group in the presence of said compound compared to the expression of said second reporter group in the absence of said compound.