Patent ID: 11913944
Assignee: PROMEGA CORPORATION
Field: Measurement (Instruments)
Classification: CPC G | IPC G

Claim 9:
10. A method of measuring photocrosslinking efficiency of a bioagent-linked PRG, comprising:
contacting sample comprising a target molecule with a PRG/HA probe of claim 1, wherein the bioactive agent is capable of binding the target molecule and wherein the target molecule is tethered to a bioluminescent reporter;
allowing the bioactive agent to bind the target molecule;
irradiating the sample with a wavelength of light that converts the PRG into a reactive form of the PRG, wherein the reactive form of the PRG is capable of forming a covalent bond with the target molecule;
contacting the sample with a BRET reagent comprising the bioactive agent covalently tethered to a fluorophore, wherein the emission spectrum of the bioluminescent reporter overlaps with the excitation spectrum of the fluorophore
contacting the sample with a bioluminescent reporter substrate;
detecting light emission within emission spectra of the bioluminescent reporter and the fluorophore, wherein light emission from the fluorophore is the result of bioluminescence resonance energy transfer (BRET) from the bioluminescent reporter to the fluorophore.