Patent ID: 11970728
Assignee: HENAN AGRICULTURAL UNIVERSITY
Field: Measurement (Instruments)
Classification: CPC C  G  A | IPC A  C

Claim 3:
4. The method according to claim 1, wherein in step b), the step of cultivating the wheat seedlings comprises steps of:
i) placing wheat seeds in Petri dishes with a diameter of 9 cm, in which two layers of sterilized filter paper and 4 mL of sterilized distilled water have been added in advance, and placing 5 wheat cultivars or lines in each Petri dish with 15 grains for each cultivar or line;
ii) placing the Petri dishes with the wheat seeds in a 25° C. incubator and culturing the wheat seeds for 3 days in the dark, removing wheat grains with inconsistent germinability, adding 10 mL of sterilized distilled water to each Petri dish, and removing covers of the Petri dishes;
iii) after culturing the wheat seeds for 3 days in the incubator, continuing to incubate the wheat seeds for 7 additional days by alternating a temperature and light cycle in the incubator between a first cycle comprising a temperature of 25° C. and a light duration of 11 hours and a second cycle comprising a temperature of 21° C. and a dark duration of 13 hours.