Patent ID: 11913138
Assignee: ARIZONA BOARD OF REGENTS ON BEHALF OF ARIZONA STATE UNIVERSITY
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 0:
1. A method for affinity selection with arrayed target material, comprising the steps of:
a) providing an array comprising arrayed target proteins, wherein the arrayed target proteins comprise MAP2K5, CTBP1, SARA1A, CDK2 and RPS6KA3;
b) incubating a ribosome display material with the array,
c) washing the array to remove non-binding ribosome display material from the arrayed target proteins,
d) eluting from the arrayed target material any binding ribosome display material,
e) amplifying the binding ribosome display material; and
f) performing at least a further round of affinity selection repeating steps a)-d), whereby performing at least two rounds of affinity selection according to steps a) to d) yields bound ribosome display material having the highest affinity for at least one of the arrayed target proteins; and
g) isolating pools of the bound ribosome display material to identify ribosome display material having the highest affinity for the at least one of the arrayed target proteins.