Patent ID: 11898208
Assignee: THE CHINESE UNIVERSITY OF HONG KONG
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 0:
1. A method for prognostication of hepatocellular carcinoma survival using a biological sample from an individual suffering from hepatocellular carcinoma, the method comprising:
(a) obtaining DNA from the biological sample, wherein the biological sample is selected from the group consisting of blood, plasma, serum, saliva, and urine;
(b) digesting the DNA with one or more methylation-sensitive restriction enzymes, wherein the one or more methylation-sensitive restriction enzymes preferentially cleave DNA sequences when present in an unmethylated state than in a methylated state;
(c) detecting a target sequence in the DNA digested in (b), wherein the target sequence comprises at least one segment of RASSF1A promoter sequence comprising at least two methylation-sensitive restriction enzyme recognition sites; and
(d) prognosticating hepatocellular carcinoma survival based on the level of the target sequence determined in (c).