Patent ID: 11857578
Assignee: PIERRE FABRE DERMO-COSMETIQUE
Field: Pharmaceuticals (Chemistry)
Classification: CPC A  C | IPC A

Claim 0:
1. Bacterial extract of a non-pathogenic Gram-negative bacterium belonging to the class Betaproteobacteria, subfamily Neisseriaceae, comprising a 16S rRNA comprising sequence SEQ ID NO: 1, or any sequence having at least 80% identity with sequence SEQ ID NO: 1, said bacterial extract being obtained by a process consisting of the steps of:
a. culture of said bacterium in a suitable medium to obtain a bacterial culture;
b. liquid phase/solid phase separation of said bacterial culture and removal of the liquid phase;
c. cell lysis of the solid phase,
d. resuspension of the lysed solid phase in an aqueous liquid phase, wherein the aqueous liquid phase is a basic buffer comprising at least one of Tris buffer, arginine buffer and Tris-arginine buffer,
e. liquid phase/solid phase separation and recovery of the liquid phase, wherein the liquid phase comprises the soluble cytoplasmic intracellular compounds of the bacteria,
f. optional filtration of the liquid phase.