Patent ID: 11952637
Assignee: THE GOVERNING COUNCIL OF THE UNIVERSITY OF TORONTO
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 0:
1. A method of detecting a viral SARS-CoV-2 RNA in a sample from a subject, the method comprising the steps of:
(a) heating the sample, whereby viral RNAs are released from viral particles present in the sample;
(b) amplifying the released viral RNAs, wherein amplifying comprises isothermal amplification and produces a target nucleic acid; and
(c) contacting the amplified target nucleic acid to one or more riboregulators, wherein each riboregulator comprises:
(i) a first region that is complementary to at least a portion of the target nucleic acid or a reverse complement thereof;
(ii) a second region that encodes a reporter protein and translation regulatory sequences;

wherein the contacting occurs under conditions that allow translation of the reporter protein in the presence of the target nucleic acid or reverse complement thereof, but not in the absence of the target nucleic acid or reverse complement thereof; and
(d) detecting the reporter protein as an indicator that the viral SARS-CoV-2 RNA is present in the subject sample;
wherein each riboregulator first region comprises, independently, one of SEQ ID NOs: 26-51.