Patent ID: 11945846
Assignee: DANA-FARBER CANCER INSTITUTE, INC.
Field: Pharmaceuticals (Chemistry)
Classification: CPC C  A  Y | IPC A  C

Claim 25:
26. A method of making a stapled peptide or pharmaceutically acceptable salt thereof, the method comprising:
(a) providing a peptide comprising the amino acid sequence:
TRSSRAGLQWPVGRVX1RLLX2K (SEQ ID NO:38), TRSSRAGLQWPX1GRVX2RLLRK (SEQ ID NO:42), TRSSRAGLX1WPVX2RVHRLLRK (SEQ ID NO:43), TRX1SRAX2LQWPVGRVHRLLRK (SEQ ID NO:47), TRSSRAX3LQWPVGX4VHRLLRK (SEQ ID NO:55), or TRSSX3AGLQWPX4GRVHRLLRK (SEQ ID NO:57), wherein each of X1, X2, X3, and X4 is an α, α-disubstituted non-natural amino acid comprising an olefinic side chain; and
(b) performing a ring-closing metathesis reaction on the peptide, thereby making the stapled peptide.