Patent ID: 11952604
Assignee: PEPSICO, INC.
Field: Biotechnology (Chemistry)
Classification: CPC C  Y | IPC C

Claim 0:
1. An enzymatic method for preparing Rebaudioside J, the method comprising reacting Rebaudioside A with a rhamnosyl donor in a reaction system comprising: recombinant cells comprising a UDP-glycosyltransferase, a UDP-glycosyltransferase prepared therefrom, or both the recombinant cells comprising the UDP-glycosyltransferase and the UDP-glycosyltransferase prepared therefrom, wherein the UDP-glycosyltransferase comprises an amino acid sequence having at least 80% sequence identity to SEQ ID NO: 2, wherein Rebaudioside A is converted to Rebaudioside J at a conversion rate greater than 90%.