Patent ID: 11867685
Assignee: CHEMCOM S.A.
Field: Measurement (Instruments)
Classification: CPC G | IPC G

Claim 1:
2. The method according to claim 1, said method comprising:
a) contacting a chimeric receptor that comprises the central region of OR5A2, encompassing the transmembrane domains 2 to 7 having the amino acid sequence of SEQ ID NO: 11, which is fused at its N-terminus to the N-terminal extracellular moiety, the transmembrane domain 1 and the intracellular loop 1 of a G protein-coupled receptor; and which is fused at its C-terminus to the intracellular C-terminal end of a G protein-coupled receptor, for identifying agents or samples that interfere with the binding between said OR5A2 chimeric receptor and musk compounds, with said agent or sample, wherein said chimeric receptor is capable of binding musk compounds selected from the group consisting of: nitromusks, macrocyclic musks, polycyclic musks and linear musks;
b) measuring a signaling activity of said chimeric receptor in the presence of said agent or sample; and
c) comparing the activity measured in the presence of said agent or sample to the activity measured in a reaction in which said chimeric receptor is contacted with one or more musk compound(s) at its/their EC50, wherein said agent or sample is identified as an agent or a sample, that modulates the activity of said chimeric receptor as defined herein when the amount of the activity measured in the presence of the agent or sample is at least 10% of the amount induced by said musk compound(s) at its/their EC50.