Patent ID: 11952611
Assignee: LABORATORY CORPORATION OF AMERICA HOLDINGS
Field: Measurement (Instruments)
Classification: CPC C  G | IPC C  G

Claim 20:
21. A system for detecting antibiotic resistance of bacteria in a sample, comprising
a recombinant bacteriophage specific to the bacteria, wherein the recombinant bacteriophage comprises a genetic construct inserted into a late gene region of the bacteriophage genome, the genetic construct comprising:
(i) an indicator gene, wherein the indicator gene is not contiguous with a gene encoding a structural bacteriophage protein and does not yield a fusion protein; and
(ii) a promoter controlling transcription of the indicator gene, wherein the promoter is a bacteriophage late promoter,

and wherein expression of the indicator gene results in an indicator protein product, and wherein the indicator protein product is a luciferase;
a component for contacting the sample with the antibiotic,
a component for contacting the sample with the recombinant bacteriophage specific to the bacteria; and
a component for detecting the protein product in the sample that has contacted the recombinant bacteriophage, wherein positive detection of the indicator protein product indicates the bacteria is resistant to the antibiotic.