Patent ID: 11972583
Assignee: BGI SHENZHEN
Field: Measurement (Instruments)
Classification: CPC G  C | IPC C  G

Claim 7:
8. A gene sequencing instrument, comprising:
a processor; and
a storage device storing one or more computer programs, which when executed by the processor, cause the processor to:
obtain at least one fluorescence image of a biochip;
select a preset local region of the fluorescence image, the preset local region having at least one borderline frame in a first direction and a second direction, the borderline frame comprising a plurality of image points of fluorescent molecules, and the first direction being perpendicular to the second direction;
acquire, in the preset local region, a position of a minimum value of a sum of brightness values of pixels in the first direction and the second direction, and acquire pixel-level registration points according to the position of the minimum value of the sum of brightness values of pixels;
divide the pixel-level registration points into non-defective pixels and defective pixels according to a preset classification rule;
determine whether the fluorescence image meets a preset standard according to a number of the non-defective pixels;
correct positions of the defective pixels according to positions of the non-defective pixels, if the fluorescence image meets the preset standard; and reobtain the fluorescence image if the fluorescence image does not meet the preset standard;
detect image points of fluorescent molecules on a cross line where the pixel-level registration points are located, of borderline frames in the first direction and the second direction, and acquiring a position of a center of gravity of image points of fluorescent molecules according to a center of gravity method;
fit straight lines in the first direction and the second direction respectively according to the position of the center of gravity of the image points of fluorescent molecules, a cross point of the straight line fitted in the first direction and the straight line fitted in the second direction being a sub-pixel level position of the registration point; and, acquire boundary points of the fluorescence image according to the straight lines fitted in the first direction and the second direction, and calculate positions of the boundary points.