Patent ID: 11912988
Assignee: CHINA NATIONAL RICE RESEARCH INSTITUTE
Field: Organic fine chemistry (Chemistry)
Classification: CPC C | IPC C

Claim 1:
2. A kit for constructing a simplified genomic library, wherein the kit consists of one first pair of primers for a non-specific PCR amplification of a whole genome and one second pair of primers for a specific PCR amplification of the amplified products of the first pair of primers, wherein each primer of the first pair of primers sequentially comprises a bridging sequence, a first tag sequence, and a sequence for non-specific PCR amplification in the 5′ to 3′ direction, wherein the first pair of primers is
SEQ ID NO:4 and SEQ ID NO:8;
wherein each primer of the second pair of primers sequentially comprises a sequencing adaptor sequence, a sequencing primer sequence, and the bridging sequence in the 5′ to 3′ direction.