Patent ID: 11946937
Assignee: MAYO FOUNDATION FOR MEDICAL EDUCATION AND RESEARCH
Field: Measurement (Instruments)
Classification: CPC G  A | IPC G

Claim 13:
14. A method for determining a ratio of IgM immunoglobulins to apoptosis inhibitor of macrophage (AIM) polypeptides (IgM:AIM) in a sample, the method comprising:
providing a sample comprising immunoglobulins from the patient;
immunopurifying IgM immunoglobulins from the sample, wherein the immunopurifying step comprises an elution under conditions suitable to release AIM polypeptides bound to the IgM immunoglobulins into the immunopurified sample;
subjecting the immunopurified sample to a mass spectrometry to obtain a mass spectrum of the sample;
quantifying IgM immunoglobulins, wherein said quantifying comprises identifying the presence of IgM immunoglobulins based on the multiply charged ion peaks in the spectrum corresponding to the IgM immunoglobulins, and converting the peak area of the identified peaks to a molecular mass to quantify the IgM immunoglobulins in the sample;
quantifying AIM polypeptides, wherein said quantifying comprises identifying the presence of AIM polypeptides based on the multiply charged ion peaks in the spectrum corresponding to the AIM polypeptides, and converting the peak area of the identified peaks to a molecular mass to quantify the AIM polypeptides in the sample; and
determining the IgM:AIM ratio in the sample.