Patent ID: 11890617
Assignee: MIROCULUS INC.
Field: Chemical engineering (Chemistry)
Classification: CPC B | IPC B  G

Claim 7:
8. A method of replenishing a reaction droplet in an air gap region of a microfluidic apparatus to correct for evaporation, the method comprising:
optically monitoring the reaction droplet by using a camera to image the droplet in the air gap region of the microfluidic apparatus;
determining a volume of the reaction droplet by from the image of the droplet;
determining when the volume of the reaction droplet falls below a threshold, wherein the reaction droplet comprises a solvent and reaction reagents;
introducing a replenishing droplet into the air gap region of the microfluidic apparatus, wherein the replenishing droplet consists of solvent; and
combining the replenishing droplet with the reaction droplet after the volume of the reaction droplet falls beneath the threshold.