Patent ID: 11859239
Assignee: RICOH COMPANY, LTD.
Field: Measurement (Instruments)
Classification: CPC C  B  G | IPC B  C  G

Claim 7:
8. A method for producing a nucleic acid sample-contained container, the method comprising:
forming a plurality of dispersed phases, each of which comprises a nucleic acid that comprises: an intended base sequence; and a base sequence for detection different from the intended base sequence;
amplifying the base sequence for detection in the dispersed phases;
discriminating between positive dispersed phases in which the base sequence for detection has been amplified and negative dispersed phases in which the base sequence for detection has not been amplified among the dispersed phases;
sorting from each other, the positive dispersed phases and the negative dispersed phases discriminated between in the discriminating; and
dispensing a droplet of the positive dispersed phases sorted in the sorting into a container, wherein the container comprises a plurality of shapes selected from the group consisting of a concave bottom and a section on a substrate, wherein the droplet consists of
a solution, wherein the solution is selected from the group consisting of water, nuclease-free water, a broth, a separation liquid, a diluted solution, a buffer, an organic substance lysing liquid, an organic solvent, an electrolyte aqueous solution, an inorganic salt aqueous solution, a metal aqueous solution, a labeling agent or reagent, and a mixture liquid of these solutions;
a nucleic acid amplifying agent and a fluorescent labeling reagent; and
at least one of the intended base sequence and a plurality of the base sequence for detection, wherein the at least one of the intended and the plurality of the base sequence for detection are unbound to a particle, wherein the plurality of the base sequence for detection is in excess relative to the at least one of the intended base sequence,

wherein a number in which the intended base sequence is comprised is less than 1,000, and a coefficient of variation (CV value) for the number in which the intended base sequence is comprised is lower than 20%.