Patent ID: 11965881
Assignee: KANSAS STATE UNIVERSITY RESEARCH FOUNDATION
Field: Measurement (Instruments)
Classification: CPC G  B | IPC B  G

Claim 18:
19. A microfluidic device comprising:
a cartridge and a planar substrate retained in said cartridge, said cartridge comprising a sample inlet well formed in said cartridge above a sample application region in or on said substrate and at least one detection region in fluid communication with said sample application region via a microfluidic channel extending from said sample application region to said detection region, said microfluidic channel having a terminal end positioned distal from said inlet well, and an optional absorbent pad positioned at said terminal end, wherein said detection region comprises a nanosensor immobilized therein or thereon; and
wherein said nanosensor comprises a central carrier particle, a first particle tethered to said carrier particle via an oligopeptide linkage, and a second particle directly attached to said carrier particle, wherein said oligopeptide linkage comprises a supramolecular recognition sequence, a protease consensus sequence, a post-translationally modifiable sequence, or a sterically hindered benzylether bond for specific interaction with a biological marker, wherein at least one of said first and second particles is a detectable particle and the other of said first and second particles is a quencher particle.