Patent ID: 11859203
Assignee: UCB BIOPHARMA SRL
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 0:
1. A method for reducing the heterogeneity of a population of recombinant proteins produced in cell culture, said method comprising growing host cells producing a recombinant protein in a cell culture medium wherein the cell culture medium comprises one or more cysteine/cystine analogs, wherein said reduction of heterogeneity comprises reducing:
a) intensity of color;
b) charge heterogeneity, whereby the main charge species does not decrease; and/or
c) amino acid oxidation,
wherein the one or more cysteine/cystine analogs are selected from the group consisting of N,N′-diacetyl-L-cystine-dimethylester, N-acetyl-L-cysteine and N,N′-diacetyl-L-cystine, wherein the recombinant proteins are antibodies or antigen binding fragments thereof, and wherein the host cells are mammalian cells.