Patent ID: 11920177
Assignee: SASYA INC.
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 16:
17. A composition comprising: βHIV produced by a non-natural microorganism, wherein the non-natural microorganism comprises a non-natural enzyme, wherein the non-natural enzyme has been modified or mutated to increase the ability of the enzyme to preferentially utilize α-kaetoisocaproate as its substrate, and wherein the non-natural enzyme comprises one or more modifications or mutations at substrate-specificity positions corresponding to amino acids selected from A361, F336, F347, F364, F368, F371, G362, I227, I252, L224, L289, L323, L367, N187, N241, N363, P239, Q251, Q265, S226, V212, V217, V228 and W210 of any of SEQ ID NO: 1 or SEQ ID NO: 6, wherein the αHIV prior to any isolation or purification process has not been in substantial contact with any halogen-containing component, the halogen-containing component comprising hydrochloric acid or chloroform.