Patent ID: 11970515
Assignee: CHENGDU NEWSUN CROP SCIENCE CO., LTD.
Field: Basic materials chemistry (Chemistry)
Classification: CPC C  A | IPC A  C

Claim 1:
2. A preparation method of the crystal form of the BR analogue according to claim 1, wherein a preparation process of the crystal form I comprises the following steps: 1) extracting a crude extract of a natural Brassinosteroid (BR) analogue from rapeseed pollen; 2) dissolving 10 parts to 20 parts of the crude extract of the natural BR analogue in 20 to 30 times a methanol reagent by mass, heating to promote complete dissolution, filtering an obtained solution I into a test tube while the solution I is hot, sealing the test tube with a parafilm, piercing holes in the parafilm, cooling, and allowing the solution I to stand to conduct volatilization, to obtain a precipitated bulk white crystal I, namely the crystal form I of the BR analogue; a preparation process of the crude extract includes the following steps: 1) extracting crushed rapeseed pollen with 80% to 100% by volume concentration (V/V) of an ethanol aqueous solution, conducting solid-liquid separation, retaining a resulting filtrate (optionally further concentrating the filtrate) to obtain an alcohol-soluble extract; 2) mixing the alcohol-soluble extract with 0% to 60% (V/V) of an ethanol aqueous solution, extracting with ethyl acetate, retaining a resulting ethyl acetate layer and adding esterase to allow an incomplete reaction, and drying to obtain an ester-soluble extract; and 3) loading the ester-soluble extract on a silica gel chromatographic column, conducting elution with a mixture of methanol and ethyl acetate, collecting an obtained eluate containing a natural BR analogue, and drying to obtain a crude extract of the natural BR analogue.