Patent ID: 11959137
Assignee: THE TRUSTEES OF COLUMBIA UNIVERSITY IN THE CITY OF NEW YORK
Field: Organic fine chemistry (Chemistry)
Classification: CPC C | IPC C

Claim 0:
1. A method of sequencing, said method comprising:
contacting a double stranded nucleic acid molecule comprising a primer oligonucleotide hybridized to a template polynucleotide with a first nucleotide analogue and a first polymerase thereby binding said first nucleotide analogue to a complementary nucleotide of said double-stranded nucleic acid molecule, wherein said first nucleotide analogue comprises multiple detectable moieties and wherein said first nucleotide analogue is not incorporated into the primer oligonucleotide;
detecting the detectable moiety; and
contacting the primer oligonucleotide with a second nucleotide analogue and a second polymerase thereby binding said second nucleotide analogue to a complementary nucleotide of said template polynucleotide and incorporating said second nucleotide analogue into the primer oligonucleotide, wherein the second nucleotide analogue is not associated with a detectable moiety;
further comprising removing the first nucleotide analogue before contacting the primer oligonucleotide with the second nucleotide analogue.