Patent ID: 11857616
Assignee: INSTITUT PASTEUR
Field: Pharmaceuticals (Chemistry)
Classification: CPC A  C  G  Y | IPC A  C

Claim 22:
23. A process to rescue recombinant infectious measles virus-Zika virus (MV-ZIKV) particles expressing at least (i) the precursor of membrane (prM) protein of a ZIKV, and the envelope (E) protein of a ZIKV or a truncated version thereof, or (ii) the E protein of a ZIKV or a truncated version thereof, and ZIKV Virus Like Particles (VLPs) expressing the same ZIKV protein(s), comprising:
1) co-transfecting helper cells that stably express T7 RNA polymerase, and measles N and P proteins with (i) the transfer vector plasmid according to claim 14 and with (ii) a vector, encoding the MV L polymerase;
2) cultivating said co-transfected helper cells in conditions enabling the production of recombinant MV-ZIKV particles;
3) propagating the thus produced recombinant MV-ZIKV particles by co-cultivating said helper cells of step 2) with cells enabling said propagation;
4) recovering recombinant infectious replicating MV-ZIKV particles expressing at least (i) the prM protein of the ZIKV, and the E protein of the ZIKV or the truncated version thereof, or (ii) the E protein of the ZIKV or the truncated version thereof, and ZIKV VLPs expressing the same ZIKV protein(s).