Patent ID: 11971414
Assignee: WISCONSIN ALUMNI RESEARCH FOUNDATION
Field: Measurement (Instruments)
Classification: CPC G | IPC G

Claim 0:
1. A cancer biomarker capture surface comprising
a substrate having a surface,
a first plurality of nanoparticles directly attached to the substrate surface,
a plurality of methoxy poly(ethylene glycol) polymer brush molecules attached to a first portion of the first plurality of nanoparticles on the substrate surface via an N-hydroxysuccinimide (NHS) functionality, wherein the plurality of polymer brush molecules comprise a single amine functionality for attachment to the first plurality of nanoparticles, do not comprise capture agent, and reduce nonspecific binding to the surface,
a plurality of poly(ethylene glycol) bifunctional tethers, wherein an amine functionality on a first end of the bifunctional tethers is attached to a second portion of the first plurality of nanoparticles on the substate surface via an NHS functionality, wherein the bifunctional tethers have a higher molecular weight than the polymer brush molecules,
a second plurality of amine functionalized nanoparticles attached via an NHS functionality of at least a portion of the plurality of the second ends of the bifunctional tethers, wherein the bifunctional tether connects the first plurality of nanoparticles on the substrate surface to the second plurality of nanoparticles, and
a capture agent for the cancer biomarker attached to at least a portion of the second plurality of nanoparticles via an NHS functionality,
wherein the first plurality of nanoparticles and the second plurality of nanoparticles comprise the same or a different poly(amidoamine) dendrimer (PAMAM dendrimer).