Patent ID: 11920143
Assignee: NORTHWEST A&F UNIVERSITY
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 2:
3. The method according to claim 2, wherein, constructing the NbPPR1-deficient Nicotiana benthamiana through VIGS comprises the following steps:
1) Inserting an NbPPR1-specific fragment of SEQ ID NO: 1 into a tobacco rattle virus RNA2-based plasmid (pTRV2) to obtain a silencing vector;
2) Transforming a tobacco rattle virus RNA1-based plasmid (pTRV1) and the silencing vector respectively into Agrobacterium tumefaciens to obtain a first recombinant Agrobacterium tumefaciens and a second recombinant Agrobacterium tumefaciens; and
3) Transforming the first recombinant Agrobacterium tumefaciens and the second recombinant Agrobacterium tumefaciens into Nicotiana benthamiana for joint transient expression.