Patent ID: 11906527
Assignee: AMBERGEN, INC.
Field: Measurement (Instruments)
Classification: CPC G  C  Y | IPC C  G

Claim 0:
1. A multiplex method for co-detecting a plurality of different types of biomarkers in a tissue sample on a single slide, said method comprising:
a) providing a tissue sample on a single slide;
b) contacting said tissue sample with a plurality of different antibodies to effect binding of the antibodies to the tissue sample, each of said antibodies reactive with a different biomarker, and each of said antibodies conjugated to a unique photocleavable mass-tag;
c) illuminating said photocleavable mass-tags with light so as to photocleave at least a portion of said mass-tags prior to step d); and
d) detecting, using mass spectrometric imaging, said mass-tags, or fragments thereof, as molecular ions;
where said antibodies conjugated to a mass-tags have the following general structure,