Patent ID: 11965164
Assignee: DUKE UNIVERSITY
Field: Pharmaceuticals (Chemistry)
Classification: CPC C  A | IPC A  C

Claim 0:
1. A method of making an amphiphilic polynucleotide that can self-assemble into a nanoparticle, the method comprising
combining a terminal deoxynucleotidyl transferase (TdT), an aptamer initiator having a secondary structure, and a non-natural cytostatic deoxynucleoside triphosphate (dNTP) monomer in a buffer to provide a first reaction mixture;
incubating the first reaction mixture;
adding a non-natural hydrophobic dNTP monomer to the first reaction mixture to provide a second reaction mixture; and
incubating the second reaction mixture to provide a single-stranded amphiphilic polynucleotide comprising, in a 5′ to 3′ direction:
an aptamer portion;
a first nucleotide portion comprising a nucleotide sequence of (FdUTP)m (SEQ ID NO:9), wherein m is 100 to 2,000; and
a second nucleotide portion comprising a nucleotide sequence of (Y1)n (SEQ ID NO:11), wherein Y1 is a non-natural hydrophobic nucleotide including a base having a Log P≥1.95, and n is 2 to 10.