Patent ID: 11944667
Assignee: BIOTHERAPY SERVICES LIMITED
Field: Pharmaceuticals (Chemistry)
Classification: CPC A | IPC A

Claim 0:
1. A method of making a wound treatment composition, wherein the method comprises;
i) fractionating, in a centrifuge, a human whole blood sample into multiple samples including a platelet rich plasma (PRP) sample, a platelet poor plasma (PPP) sample and an erythrocyte sample, wherein the PRP sample has a haematocrit level of 1-10%,
ii) removing the PRP sample and the PPP sample from the centrifuge,
iii) processing a portion of the PPP sample to facilitate cleavage of autologous pro-thrombin present in the PPP sample to produce autologous thrombin, and
iv) combining the PRP sample with a portion of the PPP sample and a portion of the thrombin produced in step (iii) to produce the wound treatment composition; and
wherein the processing step to facilitate cleavage of the autologous pro-thrombin from the PPP sample to produce autologous thrombin is carried out at a temperature of less than 15° C., and
wherein the wound treatment composition provides for prolonged release of growth factors, and has a final hematocrit level of 0.5 to 10% and
wherein the wound treatment composition is suitable for use by 35 minutes from obtaining the human whole blood sample.