Patent ID: 11866729
Assignee: MILTENYI BIOTEC B.V. &CO.KG
Field: Pharmaceuticals (Chemistry)
Classification: CPC C  A  G | IPC A  C  G

Claim 0:
1. An in vitro method for obtaining a cell population enriched for cells that have a protein expression profile characteristic of ventral midbrain patterned dopaminergic progenitor cells, the method comprising:
a) differentiating a sample of a line of pluripotent and/or multipotent stem cells into a neural cell preparation that contains dopaminergic progenitor cells;
b) dissociating the neural cell preparation into a suspension of individual cells;
c) sorting the individual cells in the suspension using an antigen binding molecule specific for CD117 (c-KIT) into CD117 positive cells and CD117 negative cells; and
d) harvesting the CD117 positive cells from step (c) to obtain said cell population;
wherein at least 70% of the cells in the cell population harvested in step (d) have said protein expression profile characteristic of ventral midbrain patterned dopaminergic progenitor cells.