Patent ID: 11891600
Assignee: ILLUMINA, INC.
Field: Biotechnology (Chemistry)
Classification: CPC C  G | IPC C  G

Claim 0:
1. A sample-indexed nucleic acid library preparation, comprising: a first nucleic acid library prepared from a first sample, wherein the first nucleic acid library comprises a first plurality of nucleic acid fragments, wherein each nucleic acid fragment of the first plurality comprises at least two different index sequences selected from a first set of index sequences that are all distinguishable from one another, the first set of index sequences comprising a first 5′ subset and a first 3′ subset, wherein the first set of index sequences are arranged on individual nucleic acid fragments of the first plurality such that index sequences of the first 5′ subset are located 5′ of a target sequence and not 3′ of the target sequence, and index sequences of the first 3′ subset are located 3′ of the target sequence and not 5′ of the target sequence, wherein the first plurality comprises more than one combination of the two different index sequences such that one or more individual fragments of the first plurality of nucleic acid fragments have respective different 5′ index sequences and respective different 3′ index sequences relative to one another, wherein more than one fragment of the first plurality has a same combination of the two different index sequences and different target sequences, and wherein the same combination comprises an index sequence of the first 5′ subset and an index sequence of the first 3′ subset; and
a second nucleic acid library prepared from a second sample, wherein the second nucleic acid library comprises a second plurality of nucleic acid fragments, wherein each nucleic acid fragment of the second plurality comprises at least two different index sequences selected from a second set of unique index sequences that are all distinguishable from one another and from the first set of index sequences, the second set of unique index sequences comprising a second 5′ subset and a second 3′ subset, wherein the second set of index sequences are arranged on individual nucleic acid fragments of the second plurality such that index sequences of the second 5′ subset are located 5′ of a target sequence and not 3′ of the target sequence and index sequences of the second 3′ subset are located 3′ of the target sequence and not 5′ of the target sequence wherein the first set of index sequences are associated with the first sample and not the second sample and wherein the second set of index sequences are associated with the second sample and not the first sample, wherein the second plurality comprises more than one combination of the two different index sequences such that one or more individual fragments of the second plurality of nucleic acid fragments have respective different 5′ index sequences and respective different 3′ index sequences relative to one another, and wherein more than one fragment of the second plurality has a same combination of the two different index sequences and different target sequences, wherein the same combination comprises an index sequence of the second 5′ subset and an index sequence of the second 3′ subset.