Patent ID: 11898181
Assignee: DOW GLOBAL TECHNOLOGIES LLC
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 10:
11. A process for preparing a C7-C11 2-ketoacid, the process comprising: (I) providing at least one of a C4-C10 2-ketoacid substrate with:
(A) at least one isopropylmalate synthase enzyme having isopropylmalate synthase activity;
(B) at least one isopropylmalate dehydrogenase enzyme having isopropylmalate dehydrogenase activity; and
(C) at least one genetically modified LeuCD′ enzyme complex comprising the genetically modified LeuCD′ enzyme complex according to claim 1;
under conditions that the at least one of the C4-C10 2-ketoacid substrate is converted to the C7-C11 2-ketoacid, wherein:
the at least one genetically modified LeuCD′ enzyme complex has activity against 2-hexylmalate (2-HM), and
conversion of the at least one of the C4-C10 2-ketoacid substrate to the C7-C11 2-ketoacid occurs via one or more biochemical reactions.