Patent ID: 11965197
Assignee: RENSSELAER POLYTECHNIC INSTITUTE
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 7:
8. A method of producing a phenylpropanoic acid in a TAL module cell, wherein the TAL module cell is a microbial cell comprising an exogenous gene encoding for a tyrosine ammonia lyase (TAL);
the method comprising:
providing a substrate to the TAL module cell, wherein the substrate comprises glucose, glycerol, or a combination thereof;
culturing the TAL module cell under conditions permitting synthesis of the phenylpropanoic acid by the TAL module cell; and
isolating the phenylpropanoic acid synthesized by the TAL module cell;
wherein the exogenous gene encoding for TAL encodes a polypeptide having at least 85% amino acid identity with the amino acid sequence of Rhodotorula glutinis tyrosine ammonia lyase (RgTAL) of SEQ ID NO: 29, Rhodobacter capsulatus TAL of SEQ ID NO: 80, Rice TAL of SEQ ID NO: 81, Parsley TAL of SEQ ID NO: 82, Tomato TAL of SEQ ID NO: 83, Arabidopsis TAL of SEQ ID NO: 84, or a combination thereof, having TAL activity.