Patent ID: 11859163
Assignee: ADVA BIOTECHNOLOGY LTD.
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 0:
1. A method of growing cells, comprising:
obtaining a bioreactor,
wherein the bioreactor comprises a closed vessel,
wherein the closed vessel comprises a first chamber and a second chamber upstream of and in fluid communication with the first chamber,
wherein the second chamber comprises an increasing transversal cross sectional area
 from a bottom end of the second chamber adjacent the first chamber
 to a top end of the second chamber which is opposite the bottom end;

introducing a plurality of cells into the second chamber;
introducing a fluid, through a fluid inlet port, into the first chamber,
wherein the fluid comprises a nutrient;

flowing the fluid in a first direction, from the first chamber toward the second chamber, through a first plurality of pores, the first plurality of pores aligning the fluid, controlling a velocity of the fluid, and preventing passage of a bubble through the first plurality of pores, to thereby provide an aligned fluid;
flowing the aligned fluid in only the first direction through a second plurality of pores into the second chamber, the second plurality of pores above the first plurality of pores, the second plurality of pores preventing the plurality of cells in the second chamber from passing from the second chamber into the first chamber;
decreasing the velocity of the aligned fluid as the fluid flows from the bottom end of the second chamber toward the top end of the second chamber, due to the increasing transversal cross sectional area of the second chamber, to thereby provide a reduced velocity fluid;
suspending at least some cells of the plurality of cells in the reduced velocity fluid, to thereby provide suspended cells;
growing the suspended cells with the nutrient, to thereby provide grown cells; and
removing at least a portion of the reduced velocity fluid, through a fluid outlet port, from the second chamber, to thereby provide a removed fluid.