Patent ID: 11931699
Assignee: ISTANBUL TEKNIK UNIVERSITESI
Field: Chemical engineering (Chemistry)
Classification: CPC B | IPC B

Claim 0:
1. A production method of aquaporin Z integrated membranes using different hollow fiber reinforced membranes, wherein the different hollow fiber reinforced membranes comprise a reinforced support layer containing 16% polysulfone, 10% polyvinyl pyrrolidone, Molecular weight: 40 kilo Daltons (kDa kilo Daltons), 74% n-methyl pyrrolidone, and polyester (PET) textile yarn, the production method comprising the following steps:
dissolving dioleoylphosphocholine lipid in chloroform,
using the dioleoylphosphocholine lipid and evaporating the chloroform present in a solution having 0.1% dioleoylphosphocholine concentration by weight in a nitrogen medium,
adding 10 mM phosphate buffer solution into the dioleoylphosphocholine lipid,
adding 1% aquaporin concentration by weight into liposomes,
adding 1% dodecylmaltoside detergent into the liposomes, for increasing a reconstitution process of aquaporins into the liposomes to obtain aquaporin reconstituted liposomes,
using biobeads consisting of neutral, porous styrene divinylbenzene beads to remove the 1% dodecylmaltoside detergent from the nitrogen medium,
passing the solution through 200 nm polytetrafluoroethylene membranes by using a mini extruder to ensure the aquaporin reconstituted liposomes have similar sizes,
using an interfacial surface polymerization technique to provide a polyamide layer between two phases to settle an aquaporin protein into three different support layers, wherein the three different support layers are an only polymeric layer, a nanocomposite layer and a reinforced layer,
dissolving 2% piperazine in water and 0.2% trinnesoyl chloride in cyclohexane to carry out the interfacial surface polymerization technique,
adding the aquaporin reconstituted liposomes, wherein the aquaporin reconstituted liposomes were prepared as 0.1%, into the solution with the 2% piperazine to obtain a mixed solution of the 2% piperazine and the aquaporin reconstituted liposomes,
immersing the different hollow fiber reinforced membranes initially into the mixed solution for 2 minutes,
passing the different hollow fiber reinforced membranes through 1 atmosphere pressure nitrogen gas for 1 minute to remove non-reacted piperazine monomers available on a surface from a membrane,
leaving the different hollow fiber reinforced membranes obtained in cyclohexane solution for 1 minute and immersing the different hollow fiber reinforced membranes into a trimesoyl chloride solution and leaving the different hollow fiber reinforced membranes here for 1 minute, and keeping the different hollow fiber reinforced membranes in a drying oven for 5 minutes at 50° C., and
obtaining an aquaporin Z integrated membrane.