Patent ID: 11904012
Assignee: SANOFI PASTEUR INC.
Field: Pharmaceuticals (Chemistry)
Classification: CPC A  C | IPC A  C

Claim 0:
1. A method of immunizing a subject against seasonal and pandemic influenza virus, comprising administering to the subject a pharmaceutical composition comprising a recombinant influenza hemagglutinin (HA) polypeptide, wherein the recombinant influenza HA polypeptide comprises:
an engineered head region derived from an engineered influenza HA polypeptide with a first immune profile, wherein the engineered head region is a segment encompassed by amino acid residues 59-292 of the engineered influenza HA polypeptide according to California 09 Numbering (CA09 Numbering); and
a stem region derived from a second influenza HA polypeptide with a second immune profile that is different from the first immune profile, wherein the stem region comprises amino acid residues 18-58 and 293-519 of the second influenza HA polypeptide according to CA09 Numbering,
wherein the engineered head region comprises:
one or more amino acid substitutions, deletions or insertions at one or more putative N-linked glycosylation sites such that the one or more putative N-linked glycosylation sites are disrupted or one or more additional N-linked glycosylation sites are inserted; and
one or more amino acid substitutions in the region of or adjacent to the Receptor Binding Site (RBS) region and/or an insertion of a Lysine (K) or Arginine (R) residue in a loop or loops bounding the RBS region.