Patent ID: 11959841
Assignee: UNIVERSITY OF ROCHESTER
Field: Measurement (Instruments)
Classification: CPC G  B  C | IPC B  C  G

Claim 0:
1. A device for isolating extracellular vesicles from biofluids, the device comprising:
a nanoporous membrane comprising a first surface with a plurality of pores;
wherein the pores have a generally circular shape and a range of pore diameters between 20 nanometers and 120 nanometers and are configured to size selectively capture and retain the extracellular vesicles;
a tangential fluid flow device for creating a tangential fluid flow velocity of a biofluid across the surface of the nanoporous membrane having a plurality of pores;
a pressure gradient device in fluid communication with the biofluid wherein the pressure gradient device creates a pressure gradient through the nanoporous membrane;
wherein the pressure gradient device is selected from the group consisting of a pump, a diaphragm, a vacuum device, a thermoelectric device, and a peltier device; and wherein the device for isolating extracellular vesicles from biofluids promotes blocking of the pores with extracellular vesicles to create an intentionally fouled nanoporous membrane.