Patent ID: 11913072
Assignee: ROCHE MOLECULAR SYSTEMS, INC.
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 0:
1. A method of providing treatment for an individual with cancer comprising:
(a) obtaining a plasma or formalin fix paraffin embedded tissue (FFPET) sample comprising RNA from the individual;
(b) carrying out a reverse transcription reaction on the RNA to produce cDNA;
(c) carrying out an amplification reaction comprising contacting the cDNA with (i) a primer set and a first probe labeled with a first label that specifically amplify and detect MET exon 13-14 junction, (ii) a primer set and a second probe labeled with a second label that specifically amplify and detect MET exon 14-15 junction, and (iii) a primer set and a third probe labeled with a third label that specifically amplify and detect MET exon 13-15 junction;
(d) detecting the presence of a MET exon 14 deletion if an amplification product is formed and detected by the primer set and the third probe of (iii); and
(e) providing treatment for the individual with a MET inhibitor if a MET exon 14 deletion is present;
wherein steps (b) and (c) are carried out in the same vessel, wherein the first probe is specific for the junction point of the MET exon 13-14 junction, the second probe is specific for the junction point of the MET exon 14-15 junction, and the third probe is specific for the junction point of the MET exon 13-15 junction, and wherein the third probe comprises a sequence selected from the group consisting of SEQ ID NOs: 53-55.