Patent ID: 11913951
Assignee: ALEXION PHARMACEUTICALS, INC.
Field: Measurement (Instruments)
Classification: CPC G | IPC G

Claim 0:
1. A method of detecting C3a in a biological sample, the method comprising:
(a) incubating the biological sample with a first and second antibody that bind to human C3a, wherein the first, but not the second, antibody binds to a neoepitope, and wherein the antibodies are FRET pairs; and
(b) performing fluorescence resonance energy transfer (FRET) to detect the FRET signal, thereby detecting C3a in the biological sample,, wherein the first antibody comprises heavy chain CDR1, CDR2, and CDR3 amino acid sequences as set forth in SEQ ID NOs: 1, 2, and 3, respectively, and light chain CDR1, CDR2, and CDR3 amino acid sequences as set forth in SEQ ID NOs: 4, 5, and 6, respectively.