Patent ID: 11866768
Assignee: CALIFORNIA INSTITUTE OF TECHNOLOGY
Field: Measurement (Instruments)
Classification: CPC C  G  Y | IPC C  G

Claim 0:
1. A method of unambiguously detecting any unique combination of presence or absence of a plurality of analytes, the method comprising:
(a) providing a sample from a subject comprising, or potentially comprising, said plurality of analytes,
(b) forming a mixture of said sample and hybridization probes, wherein at least one of said hybridization probes is configured to bind to at least one of said plurality of analytes corresponding with a respiratory viral pathogen, and wherein said hybridization probes further comprise at least one fluorophore and at most four fluorophores;
(c) exciting said at least one fluorophore to generate one or more signals if one or more of said plurality of analytes is present, wherein said one or more signals comprise at least one signal generated by excitement of said one or more fluorophores;
(d) measuring said one or more signals to generate a cumulative intensity measurement, wherein said cumulative intensity measurement corresponds to the presence of a unique combination of presence or absence of said plurality of analytes in said sample; and
(e) determining whether said at least one of said plurality of analytes corresponding with said respiratory viral pathogen is present, in any unique combination of presence or absence, based on said cumulative intensity measurement, wherein the method does not require any step of immobilization of said plurality of polynucleotide analytes or mass spectrometry, and
wherein each analyte is derived from a respiratory viral pathogen.