Patent ID: 11919919
Assignee: GLYCOM A/S
Field: Chemical engineering (Chemistry)
Classification: CPC C  A  B | IPC A  B  C

Claim 0:
1. A method for producing a compound Gly-A, comprising the steps of:
a) initiating an enzymatic reaction of compound A and glycosylated compound B (Gly-B) in the presence of a glycosidase capable of transferring the glycosyl (Gly) moiety to compound A;
b) allowing the reaction in step a) to reach equilibrium to form a first mixture comprising compound A, Gly-B, glycosylated compound A (Gly-A), and compound B;
c) contacting the mixture with a nanofiltration membrane with a molecular weight cut-off (MWCO) of 600-3500 Da allowing at least a part of compound B to pass and ensuring the retention of compounds Gly-A, compound A and Gly-B, wherein the active (top) layer of the membrane is composed of polyamide, and wherein the MgSO4 rejection of the membrane is 50-90%;
d) re-initiating an enzymatic reaction of compound A and Gly-B of the retentate the presence of Gly-A and a glycosidase capable of transferring the glycosyl (Gly) moiety to compound A to form a second mixture;
e) collecting the Gly-A from the second mixture;, wherein compound A is a tri- or higher oligosaccharide, Gly-A is compound A glycosylated with the Gly moiety, compound B is a disaccharide, and GlyB is compound B glycosylated with the Gly moiety, provided that compounds A and Gly-B are not identical.