Patent ID: 11884932
Assignee: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA
Field: Biotechnology (Chemistry)
Classification: CPC C  A | IPC C

Claim 0:
1. A method comprising:
a) providing primate pluripotent stem (pPS) cells in a culture medium;
b) introducing to the culture medium, factors comprising:
i) an activator of the sonic hedgehog (shh) pathway,
ii) a SMAD inhibitor, and
iii) a wnt pathway inhibitor,

to produce a cell culture enriched in MGE precursor cells;
c) culturing the MGE precursor cells to produce PSA-NCAM-negative or DLX2-negative cells; and
d) enriching for the PSA-NCAM-negative or DLX2-negative cells.