Patent ID: 11866514
Assignee: GENMAB A/S
Field: Pharmaceuticals (Chemistry)
Classification: CPC C  A  G | IPC A  C  G

Claim 24:
25. A heterodimeric antibody obtained or obtainable by a method comprising the following steps:
a) providing a first chimeric, humanized, or human homodimeric antibody comprising an Fc region, said Fc region comprising a first CH3 region of human IgG4;
b) providing a second chimeric, humanized, or human homodimeric antibody comprising an Fc region, said Fc region comprising a second CH3 region of human IgG4 comprising amino acid substitutions at positions 405 and 409, wherein the numbering is according to the EU Index,
wherein the sequences of said first and second antibody CH3 regions are different;
c) incubating said first antibody together with said second antibody under reducing conditions sufficient to allow the cysteines in the hinge region to undergo disulfide-bond isomerization; and
d) obtaining said heterodimeric antibody.