Patent ID: 11965877
Assignee: BIO-RAD LABORATORIES, INC.
Field: Measurement (Instruments)
Classification: CPC G  C | IPC C  G

Claim 9:
10. A method for barcoding RNA from a single cell, the method comprising:
forming a plurality of aqueous droplets in oil, each droplet comprising a plurality of different RNA molecules from a single cell and a plurality of nucleic acid constructs, wherein each of the nucleic acid constructs comprises a universal priming site, a sequence specific region, and a unique barcode that allows sequence reads to be assigned to one molecule from a sample;
hybridizing at least one nucleic acid construct to at least one RNA molecule in at least one of the droplets;
extending the hybridized nucleic acid construct to produce an extension product not attached to a microbead; and
amplifying, using the universal priming site, the extension product to yield an amplified product.