Patent ID: 11890396
Assignee: ADVANCED SOLUTIONS LIFE SCIENCES, LLC
Field: Medical technology (Instruments)
Classification: CPC A  B | IPC A  B

Claim 7:
8. A method of fabricating a bone graft, the method comprising:
(a) biofabricating via 3D-printing a graft core from a pre-bone constituent comprising demineralized bone matrix (DBM) and a carrier;
(b) incubating the graft core in a first culture medium comprising a crosslinker for a first incubation period;
(c) enrobing at least a portion of the graft core with a pre-vascularized shell constituent comprising from about 80,000 to about 500,000 microvessels/ml isolated intact microvessel fragments, mesenchymal stem cells, and from about 1.5 to about 7 mg/ml collagen; and
(d) incubating the enrobed graft core of step (c) in a second culture medium for a second incubation period to provide a living bone graft.