Patent ID: 11932878
Assignee: THE RESEARCH FOUNDATION FOR THE STATE UNIVERSITY OF NEW YORK
Field: Measurement (Instruments)
Classification: CPC C  G | IPC C  G

Claim 0:
1. A genetically engineered cell line comprising an immortalized cell line comprising:
i) one or more modulated metabolic genes, wherein the expression of the one or more modulated metabolic genes is greater than the expression in an unmodified control cell,
wherein the one or more modulated metabolic genes are selected from the group consisting of: CYP1A1, CYP1A2, CYP2B6, CYP2E1, and CYP3A4;

ii) one or more sgRNAs for one or more metabolic genes, wherein the one or more sgRNAs are one or more of SEQ ID NOs: 1-15 and 19-24; and
iii) an inactive Cas9 (dCas9) operatively linked to a VP64 transcriptional activator.