Patent ID: 11939628
Assignee: NEW ENGLAND BIOLABS, INC.
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 0:
1. A method, comprising:
(a) reacting 5-hydroxymethylcytosine (5hmC) in a nucleic acid sample with a glucosyl transferase in the absence of a Tet enzyme to form a nucleic acid product with a modified hmC;
(b) converting cytosine (C) to uracil and 5-methyl cytosine (5mC) to thymidine in the nucleic acid product from (a) with a protein that has at least 90% sequence homology to apolipoprotein B mRNA editing enzyme, catalytic polypeptide-like (Apobec);
(c) optionally amplifying the reaction product from (b); and
(d) hybridizing and/or sequencing the product from (b) or amplicon thereof.