Patent ID: 11857644
Assignee: HUNAN CANCER HOSPITAL
Field: Pharmaceuticals (Chemistry)
Classification: CPC A  C  Y | IPC A

Claim 1:
2. A method for preparing the multi-signal fluorescent probe of claim 1, comprising:
adding 2-methoxyphenothiazine and ethyl iodide into a mixture of dichloromethane (DCM) and acetonitrile followed by a first reaction to obtain a reaction mixture; and subjecting the reaction mixture to a first post-treatment to obtain 10-ethyl-2-methoxy-10H-phenothiazine;
adding boron tribromide into the 10-ethyl-2-methoxy-10H-phenothiazine under an inert gas followed by a second reaction under an ice bath and a second post-treatment to obtain 10-ethyl-10H-phenothiazin-2-ol; and
mixing the 10-ethyl-10H-phenothiazin-2-ol, malonic acid, zinc chloride and phosphorus oxychloride followed by a third reaction and a third post-treatment to obtain the multi-signal fluorescent probe.