Patent ID: 11946865
Assignee: BECTON, DICKINSON AND COMPANY
Field: Measurement (Instruments)
Classification: CPC G  A  C | IPC A  C  G

Claim 1:
2. A method of normalizing detector readings, the method comprising:
introducing a first reference fluid into a first reference vial comprising a first reference vial sensor comprising a fluorophore and a pH indicator, the first reference fluid exposing the first reference vial sensor in the first reference vial to a first performance boundary condition, the first performance boundary condition comprising a low pH condition or a high pH condition;
transmitting light at an excitation frequency of the first reference vial sensor to the first reference vial to cause the first reference vial sensor to emit a first reference vial fluorescence signal;
filtering the first reference vial fluorescence signal using a first emission filter and a second emission filter that is different than the first emission filter;
measuring an intensity of the first reference vial fluorescence signal that was filtered using the first emission filter and an intensity of the first reference vial fluorescence signal that was filtered using the second emission filter to generate a first reference ratio;
inoculating a blood culture test vial comprising a test vial sensor with the blood sample, the test vial sensor comprising a fluorophore and a pH indicator;
transmitting light at an excitation frequency of the test vial sensor to the test vial to cause the test vial sensor to emit a test vial fluorescence signal;
filtering the test vial fluorescence signal using the first emission filter and the second emission filter;
measuring an intensity of the test vial fluorescence signal that was filtered using the first emission filter and an intensity of the test vial fluorescence signal that was filtered using the second emission filter to generate a test vial ratio; and
normalizing the measured intensity of the test vial fluorescence signal that was filtered using the first emission filter and the intensity of the test vial fluorescence signal that was filtered using the second emission filter by comparing the test vial ratio to the first reference ratio.