Patent ID: 11913046
Assignee: INBIOSE N.V.
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 7:
8. A yeast cell, which is genetically engineered, the yeast cell comprising:
a) at least one heterologous polynucleotide encoding a transporter protein that facilitates export of 2′ fucosyllactose from the yeast cell, wherein the transporter protein is selected from the group consisting of a Sugars Will Eventually be Exported Transporter (SWEET) family transporter having at least 90% identity to a peptide selected from the group consisting of SEQ ID NO: 93, 94, 95, and 96, a Sugar Efflux Transporter A (SetA) family transporter having at least 90% identity to a peptide selected from the group consisting of SEQ ID NO: 88, 105, 106, 107, and 108, and a Sugar porter family transporter having at least 90% identity to a peptide selected from SEQ ID NO: 65 and SEQ ID NO: 66;
b) at least one heterologous polynucleotide encoding a guanosine diphosphate (GDP)-mannose-4,6-dehydratase (EC 4.2.1.47);
c) at least one heterologous polynucleotide encoding a GDP-4-keto-6-D-deoxymannose epimerase-reductase (EC 1.1.1.271); and
d) at least one heterologous polynucleotide encoding a 2-α-L-fucosyltransferase (EC 2.4.1.69);
wherein the yeast cell produces 2′ fucosyllactose.