Patent ID: 11898142
Assignee: MASSACHUSETTS INSTITUTE OF TECHNOLOGY
Field: Measurement (Instruments)
Classification: CPC C  B  G  Y | IPC B  C  G

Claim 39:
40. A method for detecting polypeptides in samples, comprising:
distributing a sample or set of samples into a set of individual discrete volumes, the individual discrete volumes comprising the polypeptide detection system of claim 2;
incubating the sample or set of samples under conditions sufficient to allow binding of the peptide detection aptamers to one or more target polypeptides, wherein binding of the aptamers to corresponding target polypeptides exposes the RNA polymerase binding sites or primer binding sites resulting in generation of one or more trigger RNAs;
activating the CRISPR effector protein having collateral cleavage activity via binding of the one or more guide RNAs to the one or more trigger RNAs, wherein activating the CRISPR effector protein results in modification of the RNA-based masking construct such that a detectable positive signal is produced; and
detecting the detectable positive signal, wherein detection of the detectable positive signal indicates a presence of one or more target polypeptides in a sample.