Patent ID: 11885779
Assignee: REGENERON PHARMACEUTICALS, INC.
Field: Measurement (Instruments)
Classification: CPC G | IPC G

Claim 8:
9. A method of creating an orthogonal partial least square (OPLS) model for detection of chromatography column deterioration, comprising:
(a) selecting a process or unit operation for which the OPLS model is being created;
(b) collecting raw data for one or more ultraviolet (UV) chromatogram traces of one or more column cycles/lots/runs for the selected process or unit operation;
(c) normalizing and aligning the collected raw data;
(d) generating artificially created curves from normalized raw data;
(e) classifying and formatting data for importation into a multivariate tool;
(f) importing classified and formatted data into the multivariate tool to generate a training set, wherein the artificially created curves are classified as “undesirable” chromatogram traces for the training set; and
(g) generating the OPLS model from the multivariate tool using the training set.