Patent ID: 11959061
Assignee: nan
Field: Measurement (Instruments)
Classification: CPC C  G | IPC C  G

Claim 0:
1. An apparatus for electroporation of biological cells, comprising:
a sample container having an insulator chamber for holding cells, the sample container having a first electrode and a second electrode providing electrical connection for electroporation, the insulator chamber being configured to contain a groove on a top of one side of the insulator chamber, the first electrode covering an inner rim of the groove on the side of the insulator chamber, and an outer rim of the groove being higher than the inner rim of the groove;
a first conductive medium layer containing an aqueous solution having solutions; and
a surface formed on the first conductive medium layer,
wherein:
the surface is configured to support at least one cell monolayer which is separated from the first electrode and the second electrode;
the first conductive medium layer directly conduct an electric pulse through the solute ions to the at least one cell monolayer through substantially the entire surface;
at least one of the first electrode and the second electrode is a movable electrode and is configured as a sealing cover confining a cell-containing sample within the insulator chamber and between the first electrode and the second electrode during the electrical pulse, and
the groove is configured to receive extra liquid.