Patent ID: 11873535
Assignee: HERBALIFE INTERNATIONAL OF AMERICA, INC.
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 10:
11. A method of authenicating a processed botanical dietary supplement, the method comprising:
isolating genomic DNA from a processed botanical dietary supplement, wherein the genomic DNA isolated from the processed botanical dietary supplement comprises botanical DNA fragments, wherein at least some of the botanical DNA fragments are less than 220 base pairs, wherein the genomic DNA isolated from the processed botanical dietary supplement is degraded and undetectable using DNA barcoding, and wherein the botanical DNA fragments are present in an amount of 1 pg to 1 ng;
ligating adapters to the botanical DNA fragments to generate adapter-ligated DNA fragments;
amplifying the adapter-ligated DNA fragments using 8-12 cycles of polymerase chain reaction (PCR);
detecting the botanical DNA fragments;
determining a size range of the botanical DNA fragments;
designing primers according to the size range of the botanical DNA fragments;
hybridizing the primers to a target nucleic acid sequence in the botanical DNA fragments; and
amplifying the target nucleic acid sequence,
wherein the processed botanical dietary supplement comprises a botanical material comprising chamomile (Matricaria chamomilla), German chamomile (Matricaria recutita), feverfew (Tanacetum parthenium), Roman chamomile (Chamaemelum nobile syn anthemis noblis), Chineses chamomile (Chrysanthemum x morifolium or Chrysanthemum indicum), guarna (Paullinia cupana), parsley (Petroselimum crispum), celery (Apium graveolens), fennel (Foeniculum vulgare), Siberian ginseng (Eleutherococcus senticosus), Dong Quai (Angelica sinensis), garden angelica (Angelica archangelica), pubescent Angelica (Angelica pubescens), dahurain angelica (Angelica dahurica), Chinese cinnamon (Cinnamomum cassia), true cinnamon (Cinnanamomum verum syn Cinnamomum zeylanicum), Indonesian cinnamon (Cinnamomum burmannii), ginkgo (Ginkgo biloba), Japanese sophora (Sophora japonica), buckwheat (Fagopyrum esculentum), jujube (Ziziphus spinosa), Indian jujube (Ziziphus mauritiana), Japanese raisin tree (Hovenia dulcis), ginger (Zingiber officinale), lesser galangal (Alpinia officinarum), greater galangal (Alpinia galanga), schisandra (Schisandra chinensis), southern schisandra (Schisandra sphenanthera), astragalus (Astragalus membranaceus), maca (Lepidium meyenii), radish (Raphanus sativus), turnip (Brassica rapa), peppermint (Mentha piperita), Chinese mint (Mentha canadensis), green tea (Camellia sinensis), rosemary (Rosmarinus officinalis), bilberry (Vaccinium myrtillus), blueberry (Vaccinium corymbosum), cranberry (Vaccinium macrocarpon), mulberry (Morus alba), or guarana (Paullinia cupana).