Patent ID: 11884986
Assignee: MONSANTO TECHNOLOGY LLC
Field: Biotechnology (Chemistry)
Classification: CPC C  A  Y | IPC A  C

Claim 0:
1. A method of determining the zygosity of a corn plant genome comprising corn event MON87403 DNA in a sample comprising:
(a) contacting the sample with a first pair of DNA molecules and a second distinct pair of DNA molecules that:
(i) when used together in a nucleic acid amplification reaction with corn event MON87403 DNA, produces a first amplicon that is diagnostic for corn event MON87403; and
(ii) when used together in a nucleic acid amplification reaction with corn genomic DNA other than corn event MON87403 DNA, produces a second amplicon that is diagnostic for corn wild type genomic DNA other than corn event MON87403 DNA;

(b) performing a nucleic acid amplification reaction; and
(c) detecting said first amplicon and said second amplicon;
wherein the first amplicon comprises a nucleotide sequence selected from the group consisting of SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:6, SEQ ID NO:7, and SEQ ID NO:8, and
wherein the presence of said first and second amplicons is diagnostic of a heterozygous genome in said sample, and wherein the presence of only said first amplicon is diagnostic of a genome homozygous for corn event MON87403 in said sample.