Patent ID: 11964997
Assignee: REALSEQ BIOSCIENCES, INC.
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 1:
2. The method of claim 1, wherein each of said plurality of sample polynucleotides comprises:
a) a 5′-end group selected from: 5′-phosphate (5′-p); 5′-OH, 5′-triphosphate (5′-ppp) or other 5′-end group that can be converted to 5′-p, 5′-OH or 5′-App before ligation to the combo adapter;
b) a 3′-end group selected from: a 3′-OH, 3′-p, 2′-p or 2′,3′-cyclic phosphate (2′,3′>p) that may be converted to 3′-OH or 3′-p or before ligation to the combo adapter; and
c) a 2′-group at its 3′-terminal residue selected from: 2′-H, 2′-OH, 2′-phosphate (2′-p) or 2′-O-methyl (2′-OMe) at the 3′-end.