Patent ID: 11965214
Assignee: PERSONALIS, INC.
Field: Pharmaceuticals (Chemistry)
Classification: CPC C  A  G  Y | IPC A  C  G

Claim 0:
1. A method for detecting a presence or recurrence of a cancer, comprising:
(a) obtaining at least a first tissue sample and a second tissue sample from a subject, wherein (i) the first tissue sample is obtained from a source that is different than the second tissue sample, (ii) the first tissue sample comprises: a benign tissue, a malignant tissue, or a mixed tissue sample, and (iii) the second tissue sample comprises a normal sample;
(b) extracting nucleic acid molecules from (i) the first tissue sample to obtain a first set of nucleic acid molecules, and (ii) the second tissue sample to obtain a second set of nucleic acid molecules;
(c) independently sequencing the first set of nucleic acid molecules and the second set of nucleic acid molecules to generate a first set of sequence reads and a second set of sequence reads, wherein the sequencing comprises whole genome sequencing;
(d) comparing with a programmed computer processor the first set of sequence reads and the second set of sequence reads to identify mosaic variants, wherein the mosaic variants are identified in sequence reads that are present in the first set of sequence reads that are not present in the second set of sequence reads;
(e) creating a first report based on an identification of the mosaic variants;
(f) obtaining one or more blood samples from the subject at a later time point than the first tissue sample and the second tissue sample;
(g) extracting nucleic acid molecules from the one or more blood samples from the subject;
(h) performing a sequencing assay on nucleic acid molecules extracted from the one or more blood samples to identify the presence or absence of the mosaic variants one or more times over the life of the subject; and
(i) providing a second report based on the presence or absence of the mosaic variants in the one or more blood samples, thereby detecting the presence or recurrence of the cancer in the subject.