Patent ID: 11912664
Assignee: PRESIDENT AND FELLOWS OF HARVARD COLLEGE
Field: Pharmaceuticals (Chemistry)
Classification: CPC C  A  G | IPC C  G

Claim 5:
6. A method for identifying the binding site of a small molecule on a protein, the method comprising:
(i) providing the compound of claim 1, or a pharmaceutically acceptable salt thereof;
(ii) activating the diazirine moiety by irradiating the compound of (i) with a specific wavelength of light;
(iii) contacting the protein with the activated compound of (ii);
(iv) forming a complex through a photo-induced covalent bond between the activated compound of (ii) and the protein;
(v) digesting the protein of the complex into constitutive peptides in the presence of a protease; and
(vi) identifying the one or more peptides produced in (iv) that is bound to the compound, thereby identifying the protein binding site of the small molecule.