Patent ID: 11872554
Assignee: ABBOTT DIAGNOSTICS SCARBOROUGH, INC.
Field: Measurement (Instruments)
Classification: CPC B  G  C | IPC B  G

Claim 0:
1. A method comprising:
providing a microfluidic device comprising: a first reaction chamber comprising a first magnet; and a second reaction chamber comprising a second magnet;
mixing a sample fluid comprising a target nucleic acid with a first set of recombinase polymerase amplification (RPA) reagents;
providing the sample fluid comprising the target nucleic acid and the RPA reagents to flail the microfluidic device, the target nucleic acid comprising a target polynucleotide sequence; and
amplifying the target polynucleotide sequence under isothermal conditions, wherein the amplifying comprises:
performing a first round of amplification on the target polynucleotide sequence in all the first reaction chamber to yield a first amplification product comprising a first amplified polynucleotide sequence;
mixing a portion of the first amplification product with a second set of RPA reagents in flail the second reaction chamber; and
performing a second round of amplification on the first amplified polynucleotide sequence in the second reaction chamber to yield a second amplification product comprising a second amplified polynucleotide sequence,

wherein the second amplified polynucleotide sequence comprises a smaller sequence completely contained within the first amplified polynucleotide sequence produced during the first round of amplification,
wherein the second amplification product is detected in less than approximately 30 minutes after providing the sample to the microfluidic device.