Patent ID: 11932847
Assignee: KAPA BIOSYSTEMS, INC.
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 0:
1. A method of fragmenting DNA comprising:
contacting input DNA and/or target DNA with a mixture of:
(a) an active transpososome, and
(b) an inactive transpososome,
under conditions suitable for transpososome activity,
wherein the active transpososome comprises a transposase derived from wild type Alishewanella aestuarii (TnAa), wherein the transposase is encoded by an amino acid sequence comprising SEQ ID NO:16,
wherein the inactive transpososome comprises a modified DNA arm, wherein the modified DNA arm comprises a phosphate at a 3′ terminal nucleotide of the modified arm,
wherein the inactive transpososome comprises a modified transposase, wherein the modified transposase comprises an amino acid sequence of SEQ ID NO:16 comprising one or more mutations, wherein the one or more mutations is a P47K mutation,
wherein the inactive transpososome and the active transpososome bind to a consensus sequence within the input and/or target DNA with imperfect complementarity, and
wherein a ratio of an amount of the inactive transpososome to an amount of the active transpososome determines a mean fragment size and a level of insertion bias.