Patent ID: 11884963
Assignee: TAKARA BIO USA, INC.
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 0:
1. A method of selectively separating one or more target nucleic acids from a composition, the method comprising:
obtaining a composition comprising both target nucleic acids and non-target nucleic acids;
contacting the composition with one or more cleavage-deficient nucleases that lack nuclease activity and one or more guide nucleic acids that guide the one or more cleavage-deficient nucleases to the one or more target nucleic acids to produce a contacted composition, wherein the contacted composition comprises the target nucleic acids, the non-target nucleic acids, the one or more cleavage-deficient nucleases, and the one or more guide nucleic acids, and the one or more cleavage-deficient nucleases, the one or more guide nucleic acids, and the one or more target nucleic acids form a complex in which the one or more guide nucleic acids specifically hybridize with the one or more target nucleic acids; and
separating the complex from the contacted composition, thereby selectively separating the one or more target nucleic acids from the composition.