Patent ID: 11965191
Assignee: CALIFORNIA INSTITUTE OF TECHNOLOGY
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 16:
17. The synthetic protein circuit of claim 1,
wherein the third protease comprising a third protease first cut site specific for the first protease, a third protease second cut site specific for the second protease, and one or more optional association domains of the third protease, wherein the third protease is configured to be deactivated by cleavage of either of the third protease first cut site specific for the first protease, the third protease second cut site specific for the second protease;
wherein the first target protein further comprises a second degron of the first target protein, a first cut site specific for the first protease, a second cut site specific for the second protease, a third cut site specific for the third protease, and a fourth cut site specific for the third protease, and wherein the target protein is stabilized by cleavage of:
the first cut site specific for the first protease and the second cut site specific for the second protease, or
both of the third and fourth cut sites specific for the third protease.