Patent ID: 11892390
Assignee: NEW YORK UNIVERSITY
Field: Measurement (Instruments)
Classification: CPC G | IPC G

Claim 15:
16. A method of characterizing a parameter of a sample by holographic microscopy, comprising the steps of:
receiving multicolor holographic image data of the sample from a storage medium;
determining a first estimate of the number of objects in the holographic image data, each object associated with a set of concentric bright and dark rings;
determining, for each set of concentric bright and dark rings, by a voting algorithm, a centroid defined by an approximate x, y position in a plane, with each pixel of the image data voting for pixels in a transformed image that may be centroids;
determining an estimate of the axial position (z) of each of the objects;
determining by Lorenz-Mie analysis an estimate of each of the objects' radius, refractive index;
using holographic image data from the sample to characterize properties of the sample and generate information characteristic of the parameters of the sample; and
analyzing the information characteristic of the sample parameters and determining in real time simultaneously size, position and refractive index of a particle of the sample.