Patent ID: 11866766
Assignee: BIO-RAD LABORATORIES, INC.
Field: Biotechnology (Chemistry)
Classification: CPC C  B | IPC C

Claim 0:
1. A method comprising:
transferring a biological sample comprising target nucleic acid material and a process reagent into a fluid pathway over an array of wells defined at a surface plane of a substrate, wherein the process reagent comprises water, wherein the biological sample has a volume from 10 microliters to 50 microliters, wherein the array of wells comprises from 10,000 wells through 250,000 wells arranged in a hexagonal close-packed array, wherein each well in the array of wells extends vertically into a planar surface of the substrate and comprises a polygonal cross section, and wherein the fluid pathway directs flow into the array of wells, linearly from a first subset of wells of the array of wells at a first end of the substrate, to a second subset of wells of the array of wells at a second end of the substrate; and
distributing a liquid immiscible with water into the fluid pathway, thereby partitioning each well of the array of wells from adjacent wells of the array of wells, at the surface plane and mitigating leakage of said target nucleic acid material of a first well to a second well of the array of wells.