Patent ID: 11932909
Assignee: SELF-SCREEN B.V.
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 0:
1. A kit for detecting HPV-induced high-grade precancerous lesion or HPV-induced invasive carcinoma or nonHiPV-induced gynaecological or anogenital cancer, said kit comprising means for the specific amplification of a bisulfite modified sequence of ASCL1 and LHX8 and detection of ASCL1 and LHX8 methylation, wherein said means comprise:
(i) a probe and primers for methylation specific PCR, wherein said probe and primers are specific for a bisulfite modified sequence of the ASCL1 nucleotide sequence of SEQ ID NO: 22 and that target a CpG rich sequence in said bisulfite modified sequence, wherein said probe and/or primers comprise a detectable label, wherein said primers comprise a first primer consisting of the sequence of SEQ ID NO: 1 or the sequence of SEQ ID NO: 16 and a second primer consisting of the sequence of SEQ ID NO: 2 or the sequence of SEQ ID NO: 17; and
(ii) a probe and primers for methylation specific PCR, wherein said probe and primers are specific for a bisulfite modified sequence of the LHX8 nucleotide sequence of SEQ ID NO: 23 and that target a CpG rich sequence in said bisulfite modified sequence, wherein said probe and/or primers comprise a detectable label.