Patent ID: 11926875
Assignee: 23IKIGAI PTE LTD
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 0:
1. A method for detecting a presence or an absence of cancer in a subject, comprising:
(a) obtaining a whole blood sample from the subject;
(b) contacting the whole blood sample with a neutral buffer and then with a salt solution, followed by performing centrifugation at a speed of between 1000 rotations per minute (rpm) and 6000 rpm, for a time period of between 5 minutes and 20 minutes, to obtain a pellet,
wherein the contacting is performed with a first ratio in a range of 1:1 to 1:20 between the whole blood sample and the neutral buffer, and
wherein the contacting is performed with a second ratio in a range of 1:2 to 1:10 between (1) the salt solution and (2) the whole blood sample contacted with the neutral buffer;
(c) washing the pellet, and re-suspending cells from the washed pellet in a buffer;
(d) performing centrifugation on the re-suspended cells, to obtain a second pellet of cells;
(e) isolating messenger ribonucleic acid (mRNA) from the second pellet of cells;
(f) assaying the isolated mRNA to (i) detect a presence of Oct-4a-expressing cells among the second pellet of cells and (ii) determine an expression level of Oct-4a;
(g) comparing the determined expression level of Oct-4a with a reference expression level; and
(h) detecting the presence or the absence of cancer in the subject based at least in part on whether the determined expression level of Oct-4a is increased by at least 10 folds as compared to the reference expression level.