Patent ID: 11959100
Assignee: KYOTO UNIVERSITY
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 10:
11. A method for proliferating neural crest cells and maintaining their multipotency, comprising the step of:
(I) suspension-culturing the neural crest cells in a medium comprising a GSK3β inhibitor and a basic fibroblast growth factor, wherein the medium comprises the GSK3β inhibitor with a concentration that exhibits an effect equivalent to that exhibited by CHIR99021 with a concentration of 3.0 μM to 4.5 μM higher than 1 μM and lower that 5 μM, thereby maintaining multipotent neural crest cells over 63 days or longer, wherein the cultured neural crest cells comprise a ratio of at least 75% or more of neural crest cells that maintain multipotency to cultured cell population.