Patent ID: 11873524
Assignee: LABORATORY CORPORATION OF AMERICA HOLDINGS
Field: Pharmaceuticals (Chemistry)
Classification: CPC C  A  G  Y | IPC A  C  G

Claim 0:
1. A method for detecting Salmonella spp. in a sample comprising:
incubating a sample comprising powdered infant formula, for 30-120 minutes with a recombinant bacteriophage derived from a Salmonella-specific bacteriophage comprising, a genetic construct inserted into a late gene region of the bacteriophage genome, the genetic construct comprising:
(i) an indicator gene; and
(ii) a promoter controlling transcription of the indicator gene, wherein the promoter is a bacteriophage late promoter;
and wherein the indicator gene is not contiguous with a gene encoding a structural bacteriophage protein and does not yield a fusion protein, and wherein expression of the indicator gene results in an indicator protein product; and
detecting the indicator protein product, wherein positive detection of the indicator protein product indicates that Salmonella spp. is present in the sample.