Patent ID: 11958881
Assignee: ZHEJIANG UNIVERSITY
Field: Chemical engineering (Chemistry)
Classification: CPC C  B | IPC B  C

Claim 2:
3. The optimization method for capturing the proteins by the MCC according to claim 1, wherein solving the optimal load residence time in step 4 comprises the following step:
solving simultaneous equations of the second formula, t
   C
  
  =
  
   
    
     A
     ×
     
      RT
      C
     
    
    
     c
     exp
    
   
   -
   
    t
    CW, of the linear relationship between the interconnected load time tC and the load residence time RTC obtained in step 2 and the third formula, t
  =
  
   
    
     t
     RR
    
    -
    
     
      (
      
       N
       -
       3
      
      )
     
     ⁢
     
      t
      CW
     
    
   
   
    (
    
     N
     -
     2
    
    ), to obtain the optimal load residence time, wherein tC (min) is the interconnected load time of the MCC, tCW (min) is the interconnected wash time of the MCC, tRR (min) is the R-R time of the MCC and comprises the sum of the washing time, the elution time, and the regeneration time, cexp (g/L) is the loading protein concentration, and N is the number of the operating columns; the optimal load residence time for capturing the proteins by the MCC under the set loading protein concentration, the set protein breakthrough percentage, and the optimal number of the operating columns is obtained by solving the simultaneous equations.