Patent ID: 11906513
Assignee: DENKA COMPANY LIMITED
Field: Measurement (Instruments)
Classification: CPC G  B | IPC B  G

Claim 6:
7. A method of measuring a sugar chain antigen in a specimen according to an immunochromatography method using the immunochromatographic test piece according to claim 1,
the method comprising
mixing the specimen with a nitrous acid solution when the immunochromatographic test piece has a region impregnated with a solid acid reagent, or mixing the specimen with an acid solution when the immunochromatographic test piece has a region impregnated with nitrite, and adding the mixture to a sample pad of the immunochromatographic test piece, wherein in the immunochromatography method,
the sugar chain antigen is extracted from the specimen by the action of nitrous acid generated through a reaction of the nitrite with the solid acid reagent in the region impregnated with the solid acid reagent or the region impregnated with the nitrite, the acid solution containing the sugar chain antigen is neutralized in a region impregnated with a neutralizing reagent, and
an antibody-sugar chain antigen-labeled antibody complex is formed in a detection region, and wherein
a speed or a direction of development of the specimen on the immunochromatographic test piece is controlled, so that a treatment with the acid reagent, the nitrite, and the neutralizing reagent is controlled.