Patent ID: 11959070
Assignee: AXAGARIUS GMBH & CO. KG
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 0:
1. A process for isolating nucleic acids from a biological sample, comprising:
(a) lysing the biological sample,
(b) adding a binding solution being free of chaotropic salts and comprising at least one organic solvent selected from the group consisting of cyclic C2-4 alkylene carbonates, C2-3 alkylene glycol diacetates, and derivatives thereof, at least one non-ionic surfactant, and nucleic acid-binding carboxylated magnetic particles as a nucleic acid-binding solid phase to the lysed sample, and separating the solid phase with the nucleic acids directly bound thereto,
(c) washing the separated solid phase including the nucleic acids bound thereto once or several times with identical or different washing solutions, and
(d) desorbing the nucleic acids from the solid phase by adding an aqueous elution buffer.