Patent ID: 11904310
Assignee: DANA-FARBER CANCER INSTITUTE, INC.
Field: Measurement (Instruments)
Classification: CPC B  C  G | IPC B  C  G

Claim 0:
1. A microfluidic device component for establishing a gradient, comprising:
a chamber defining an interior space; and
two or more inlet ports that deliver two or more different fluids to the interior space of the chamber and two or more outlet ports, wherein the two or more inlet ports comprise at least a first inlet port and a second inlet port, the two or more outlet ports comprise at least a first outlet port and a second outlet port, the first inlet port and the first outlet port are located at a first vertex of the chamber and the second inlet port and second outlet port are located at a second opposing vertex of the chamber so that the flow of the two or more fluids into the interior space results in formation of a gradient across the interior space; wherein the interior space comprises a plurality of three or more regularly spaced columns throughout the interior space and a plurality of three or more wells interspersed between the plurality of columns and wherein each well is in fluid communication with adjacent wells.