Patent ID: 11883817
Assignee: YANTAI AUSBIO LABORATORIES CO., LTD.
Field: Measurement (Instruments)
Classification: CPC B  G | IPC B  G

Claim 0:
1. A continuous two-step centrifugation method for enhanced sample enrichment in a tube for sample collection and sample processing, the tube comprising:
an upper section, an intermediate section, and a lower section; and
a volume of a density gradient medium contained in the lower section of the tube,
wherein the upper section and the lower section are substantially of cylindrical shape, the upper section has an inner diameter larger than an inner diameter of the lower section, the upper section has an outer diameter larger than an outer diameter of the lower section, and the intermediate section is located between the upper section of the tube and the lower section,
wherein a top of the intermediate section connects to a bottom of the upper section and a bottom of the intermediate section connects to a top of the lower section,
wherein an inner diameter and an outer diameter of the intermediate section decreases from the top to the bottom of the intermediate section of the tube;
wherein the intermediate section has a tapered shape;
the method comprising the steps of:
centrifuging for a first period of time at a first RCF (relative centrifugation force) without changing a relative position of the density gradient medium in the tube to achieve an initial cell separation, and
centrifuging for a second period of time at a second RCF, in order to change the relative position of the density gradient medium in the tube.