Patent ID: 11858970
Assignee: CHINA AGRICULTURAL UNIVERSITY
Field: Pharmaceuticals (Chemistry)
Classification: CPC C  A  Y | IPC A  C

Claim 4:
5. The method according to claim 4, wherein the enzyme used for the enzymolysis treatment is selected from Bacillus licheniformis; and
in step 5, the isolating and purifying comprise:
injecting the enzymolytic protein solution into the anion chromatography column, eluting with the mobile phase, and collecting the effluent in a period from 35 min to 45 min; and
injecting the effluent into the hydrophobic chromatography column, eluting with the mobile phase, and collecting the effluent in a period from 75 min to 90 min to obtain a purified product containing the oligopeptide.