Patent ID: 11873512
Assignee: NATIONAL UNIVERSITY OF SINGAPORE
Field: Pharmaceuticals (Chemistry)
Classification: CPC C  A | IPC A  C

Claim 0:
1. A method of producing a population of natural killer (NK) cells for use in treating cancer, said method comprising:
obtaining a peripheral blood sample comprising a mixed population of mononuclear cells comprising NK cells and T cells,
culturing said mixed population of mononuclear cells under culture conditions resulting in the enhanced expansion of NK cells, and
introducing into the NK cells a nucleic acid encoding a chimeric receptor, the chimeric receptor comprising:
i) an extracellular receptor domain, wherein said extracellular receptor domain comprises a fragment of NKG2D that binds one or more of MICA, MICB, ULBP1, ULBP2, ULBP3, ULBP4, ULBP5, and ULBP6, and
ii) a CD3 zeta cytoplasmic domain.