Patent ID: 11891647
Assignee: NATIVE MICROBIALS, INC.
Field: Computer technology (Electrical engineering)
Classification: CPC C  G | IPC C  G

Claim 3:
4. A method, comprising:
obtaining at least two samples sharing at least one common environmental parameter;
for an identified sample from the at least two samples detecting the presence of a plurality of microorganism strains;
determining a total quantity of microorganisms from the plurality of microorganism strains;
identifying, by sequencing DNA in the identified sample, a set of DNA representing whole genomes corresponding to the plurality of microorganism strains, each DNA representing a whole genome in the set of DNA representing whole genomes corresponding to a microorganism strain from the plurality of microorganism strains in that sample;
obtaining a set of sequencing reads, from sequencing the DNA in the identified sample, and comparing each sequencing read from the set of sequencing reads with each DNA representing a whole genome in the set of DNA representing whole genomes to identify a match between that sequencing read and a DNA representing a whole genome;
determining, based on the comparing, a relative frequency of occurrence for each microorganism strain within the identified sample;
determining an absolute cell count of each microorganism strain in the identified sample from the total quantity of microorganisms from the plurality of microorganism strains included in the microorganism type and the relative frequency of occurrence for each microorganism strain;
measuring an amount of protein for each microorganism strain by processing the protein in each sample and mapping amino acid sequences from the processing to each DNA representing a whole genome in the set of DNA representing whole genomes;
generating a gene expression profile associated with each microorganism strain based on the amount of protein associated with the microorganism strain;
determining an activity for each microorganism strain in the identified sample based on the amount of protein for that microorganism strain;
identifying active microorganism strains based on the activity for each microorganism strain from the plurality of microorganism strains in the identified sample;
selecting at least one active microorganism strain from the active microorganism strains based on the gene expression profile associated with each microorganism strain from the active microorganism strains; and
combining the at least one active microorganism strain with a carrier medium to form an active microorganism bioensemble configured to alter a property corresponding to at least one measured metadata when the active microorganism bioensemble is introduced into that target biological environment.