Patent ID: 11906522
Assignee: SHIMADZU CORPORATION
Field: Measurement (Instruments)
Classification: CPC G  H | IPC G  H

Claim 0:
1. A glycan analysis method for analyzing a structure of glycan using mass spectrometry, the glycan analysis method comprising:
a) a sample preparation step of preparing a sample by labeling glycan to be analyzed without reduction, using an acidic labeling agent having at least one site stably presenting a negative charge in a molecule with the acid labeling agent bound to the glycan; and
b) an analysis execution step of subjecting the sample to MS/MS analysis in negative ion mode, where a deprotonated ion of the glycan labeled in step a is used as a precursor ion;
c) a detecting step of detecting a product ion of D ion or E ion which reflects a branched structure of the glycan from a result of the MS/MS analysis; and
d) an estimating step of estimating a structure of the glycan based on step c,
wherein the site that stably presents a negative charge in the acidic labeling agent is a carboxy group.