Patent ID: 11898172
Assignee: GENOMATICA, INC.
Field: Biotechnology (Chemistry)
Classification: CPC C  Y | IPC C

Claim 18:
19. The cell of claim 18, wherein the cell produces one or more of the following:
(a) an increased amount of 1,3-BDO when cultured for at least 30 or 40 hours under conditions comprising a peak weight-based oxygen uptake rate of 50 mmol O2/kg/hr, 40 mmol O2/kg/hr, 30 mmol O2/kg/hr, 25 mmol O2/kg/hr, or lower, as compared to a cell in which the thiolase instead comprises the amino acid sequence of SEQ ID NO: 6;
(b) a decreased amount of pyruvate when cultured for at least 30 or 40 hours under conditions comprising a peak weight-based oxygen uptake rate of 50 mmol O2/kg/hr, 40 mmol O2/kg/hr, 30 mmol O2/kg/hr, 25 mmol O2/kg/hr, or lower, as compared to a cell in which the thiolase instead comprises the amino acid sequence of SEQ ID NO: 6; and/or
(c) 1,3-BDO and pyruvate at an increased ratio of 1,3-BDO to pyruvate when cultured for at least 30 or 40 hours under conditions comprising a peak weight-based oxygen uptake rate of 50 mmol O2/kg/hr, 40 mmol O2/kg/hr, 30 mmol O2/kg/hr, 25 mmol O2/kg/hr, or lower, as compared to a cell in which the thiolase instead comprises the amino acid sequence of SEQ ID NO: 6.