Patent ID: 11959891
Assignee: SHENZHEN SHEENVIOMICS BIOTECHNOLOGY CO., LTD.
Field: Measurement (Instruments)
Classification: CPC G  C | IPC C  G

Claim 0:
1. A method for preparing a proteomics sample, comprising:
(a) obtaining a sample solution comprising at least a protein;
(b) loading the sample solution to a preparation device, wherein the preparation device comprises:
a pipette tip;
a layer of strong cation exchange resin filler or strong anion exchange resin filler; and
a solid-phase extraction membrane, and
wherein the layer of strong cation exchange resin filler or strong anion exchange resin filler and the solid-phase extraction membrane are located in the pipette tip, and the layer of strong cation exchange resin filler or strong anion exchange resin filler is located above the solid-phase extraction membrane,
thereby retaining the protein in the layer of strong cation exchange resin filler or strong anion exchange resin filler;
(c) digesting the protein retained in the layer of strong cation exchange resin filler or strong anion exchange resin filler into a plurality of peptides;
(d) transferring the plurality of peptides to the solid-phase extraction membrane; and
(e) eluting the plurality of peptides from the solid-phase extraction membrane, thereby generating a proteomic sample.