Patent ID: 11932838
Assignee: CORNING INCORPORATED
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 0:
1. A cell culture vessel comprising:
a surface; and
a cell culture media extending material disposed on the surface, wherein the cell culture media extending material comprises:
a first layer disposed between the surface and a second layer, the first layer comprising a hygroscopic polymer and at least one sequestered nutrient;

the second layer comprising the hygroscopic polymer without the at least one sequestered nutrient, and wherein the hygroscopic polymer comprises poly(ethylene-co-vinyl acetate) (EVA);
wherein the EVA has an ability to attract and hold water molecules from the surrounding environment;
wherein the hygroscopic polymers swell to less than 50% water by weight in an aqueous environment; and
wherein the first and second layers each have a dried thickness of approximately 0.001 inches to approximately 0.002 inches.