Patent ID: 11921042
Assignee: BIOAXIAL SAS
Field: Measurement (Instruments)
Classification: CPC G | IPC G

Claim 12:
13. An optical measuring device for determining information selected from the group consisting of a spatial distribution, a location of a set of re-emitting sources disposed on a sample, and combinations thereof, the device comprising:
a first light source, whereof a wavelength is aligned to an excitation wavelength of said re-emitting source, the first light source adapted to project a compact excitation distribution;
a second light source characterized by a wavelength aligned to a wavelength, of said set of re-emitting sources, the wavelength selected from the group consisting of a depletion wavelength and an activation wavelength, the second light source adapted to project a sequence of compact light distributions of mutually distinct topology;
a scanning module for scanning the sample optically;
a detector module for detecting light re-emitted by said set of re-emitting sources;
an image acquisition module for acquiring, from the detected re-emitted light, for each scanning point, a set of images, the set having at least one member, each image of the set corresponding to one of the compact light distributions of mutually distinct topologies; and
a processor configured to execute an algorithm to reconstruct properties of the sample from the set of images, the properties selected from the group consisting of the sample itself its spatial properties, its temporal properties, its spectral properties, and combinations thereof, based on a reverse Bayesian problem leading to definition of a distribution a posteriori, by way of Bayes law, and a posteriori combining a probabilistic formulation of a noise model, as well as any a priori on a light distribution created in the sample by projection.