Patent ID: 11906721
Assignee: LEICA MICROSYSTEMS CMS GMBH
Field: Measurement (Instruments)
Classification: CPC G | IPC G

Claim 17:
18. An apparatus for manipulating at least one beam path in a microscope, the apparatus comprising:
a refractive-index determination module for determining the refractive index of a sample and/or the refractive index of an optical medium arranged in a sample volume; and
at least one beam path manipulator for setting at least one microscope parameter based on the determined refractive index for manipulation of the at least one beam path,
wherein determining the refractive index of the sample and/or of the optical medium arranged in the sample volume comprises:
(I) oblique irradiation of the sample volume with measurement light with respect to the optical axis using an optical arrangement;
(II) reflecting the irradiating light at a reflective element provided in the sample volume at a first position;
(III) imaging the reflected light onto a spatially resolving detector;
(IV) evaluating the signal detected by the detector with respect to a size and/or offset of the reflected light on the spatially resolving detector;
(V) displacing the reflective element along the optical axis to a second position and performing the method steps (III) and (IV); and
(VI) determining the refractive index based on the size and/or the offset of the reflected light for the first position and the second position of the reflective element.