Patent ID: 11885953
Assignee: ILLUMINA, INC.
Field: Audio-visual technology (Electrical engineering)
Classification: CPC G  H | IPC G  H

Claim 8:
9. A method comprising:
emitting structured illumination toward a preformed optical pattern on a targeting device;
receiving a plurality of image sets, each image set of the plurality of image sets including images captured using structured illumination microscopy (SIM) in an optical system, each image set being captured at a respective z-position from the targeting device and including captured images of the preformed optical pattern, each image from each image set having an associated channel and grating angle;
measuring a full width at half maximum (FWHM) value corresponding to each image in the plurality of image sets;
predicting values of SIM parameters of each image in the plurality of image sets, the predicting being based at least in part on the measured FWHM values; and
generating reconstructed images based at least in part on the predicted values of SIM parameters.