Patent ID: 11933729
Assignee: ABBERIOR INSTRUMENTS GMBH
Field: Measurement (Instruments)
Classification: CPC G | IPC G

Claim 13:
14. An apparatus for adapting an estimator for use in a microscope for estimating a position of an emitter in a sample based on a method, in which the sample is illuminated with light at one or more sets of probe positions and fluorescence photons are acquired for the sets of probe positions, the apparatus comprising:
illumination means configured to illuminate the sample with light at one or more sets of probe positions;
acquisition means configured to acquire fluorescence photons for the sets of probe positions;
processing means configured to add photon counts of the acquired photons to vectors of photon counts or sums of photon counts for the sets of probe positions, and to determine a value representative of background noise from the vectors of photon counts or the sums of photon counts; and
control means configured to adapt the estimator in real-time using the value representative of background noise.