Patent ID: 11946909
Assignee: ECOLE NATIONALE SUPERIEURE DE CHIMIE DE PARIS
Field: Measurement (Instruments)
Classification: CPC G | IPC G

Claim 11:
12. A method of size-based particles separation comprising injecting a sample in an asymmetric flow field-flow fractionation device, the sample comprising the particles dispersed in a liquid mobile phase and the device including:
a fractionation microchannel comprising a sample inlet and a sample outlet, the sample being injected in the sample inlet,
an auxiliary microchannel comprising an auxiliary outlet; and
a semipermeable membrane separating the fractionation microchannel and the auxiliary microchannel, said membrane being permeable to liquid and being configured to maintain the particles in said fractionation microchannel,
the fractionation microchannel being superimposed on the auxiliary microchannel, wherein the device comprises two layers, each layer containing a microfabricated recess having a thickness of less than 100 μm, the membrane being mechanically held by encapsulation in between the two layers, the recesses respectively defining the fractionation microchannel and the auxiliary microchannel on each side of the membrane.