Patent ID: 11957766
Assignee: TEXAS TECH UNIVERSITY SYSTEM
Field: Measurement (Instruments)
Classification: CPC A  G  C | IPC A  C  G

Claim 24:
25. A method of making a probe comprising a quinone propionic acid (QPA) conjugated to dicyanoisophorone (DCP) comprising:
carrying out the following reaction:, wherein the conditions are:
(a) ammonium acetate, acetic anhydride, acetic acid, toluene, reflux, 12 h;
(b) piperidine, acetonitrile, 50° C., 6 h;
(c) SnCl2, HCl, ethyl acetate, 8 h;
(d) methyl 3,3-dimethylacrylate, methanesulfonic acid, 70° C., 2 h;
(e)N-Bromosuccinimide, acetonitrile, water, room temperature, 1 h; and
(f) 1-Ethyl-3-(3-dimethylaminopropyl)carbodiimide, pyridine, rt, 10 h.