Patent ID: 11877556
Assignee: NORTH CAROLINA STATE UNIVERSITY
Field: Biotechnology (Chemistry)
Classification: CPC A  C | IPC A  C

Claim 13:
14. The Nicotiana tabacum plant of claim 1,
wherein the second mutation at the second locus comprises a Tryptophan to stop codon substitution at amino acid position 329 of a CYP82E4 nicotine demethylase, wherein the CYP82E4 amino acid position numbering is according to SEQ ID NO: 14; and
wherein the third mutation at the third locus comprises a Tryptophan to stop codon substitution at amino acid position 422 of a CYP82E5v2 nicotine demethylase, wherein the CYP82E5v2 amino acid position numbering is according to SEQ ID NO: 26.