Patent ID: 11879896
Assignee: REGENERON PHARMACEUTICALS, INC.
Field: Measurement (Instruments)
Classification: CPC G | IPC G

Claim 0:
1. A method of determining an effect of non-specific interactions on monoclonal antibody-antigen interactions in simulated blood conditions, comprising:
(a) contacting a solution comprising a blood protein and a monoclonal antibody with a biosensor, wherein a surface of the biosensor comprises an antigen that specifically binds to the monoclonal antibody, and wherein said blood protein does not specifically bind to said monoclonal antibody, wherein the blood protein is present at a concentration of between about 10 g/L and about 100 g/L;
(b) allowing the monoclonal antibody to bind to the antigen;
(c) determining an amount of the monoclonal antibody bound to the antigen using biolayer interferometry; and
(d) comparing the amount of the monoclonal antibody bound to the antigen to a control threshold to determine an effect of non-specific interactions,
wherein the control threshold is a normalized response to an ideal, dilute, or semi-dilute solution of the blood protein.