Patent ID: 11944672
Assignee: GDANSKI UNIWERSYTET MEDYCZNY
Field: Pharmaceuticals (Chemistry)
Classification: CPC A  C | IPC A  C

Claim 13:
14. The method of claim 1, wherein the Treg cells are obtained by a method comprising:
isolating T lymphocyte cells from immuno-magnetic blood samples and labeling the isolated T lymphocyte cells with monoclonal antibodies;
sorting the T lymphocyte cells according to the CD3(+)CD4(+)CD25[high]CD127(−)doublet(−)lineage(−)dead(−) phenotype using a sorting device,
wherein the sorted cells comprise a Treg cell population having a purity of at least 97%;
propagating the sorted T lymphocyte cells ex vivo with 1000 U/ml interleukin-2 and 10% autologous inactivated serum in the presence of artificial cells that present an antigen,
wherein the artificial cells are in the shape of a magnetic sphere and are coated by anti-CD3 and anti-CD28 antibodies,
wherein the artificial cells and the sorted T lymphocytes are present in a 1:1 ratio, and
wherein the ex vivo propagation time does not exceed 2 weeks; and
testing the propagated and sorted T lymphocyte cells for a FoxP3 expression level above 90%, a positive result in an IFN-gamma inhibition assay.