Patent ID: 11879162
Assignee: CALIFORNIA INSTITUTE OF TECHNOLOGY
Field: Biotechnology (Chemistry)
Classification: CPC C  Y | IPC C

Claim 20:
21. The non-transitory computer-readable storage medium of claim 1, wherein the operations further comprise: obtaining the second change in signal by:
measuring, in a sample comprising the second polynucleotide analyte, the first primer with the fluorophore attached thereto, and the second primer with the quencher attached thereto, a third signal generated by the fluorophore and the quencher;
measuring a fourth signal generated by the fluorophore and the quencher from performing one or more second polymerization reactions with the first primer and the second primer using the second polynucleotide analyte as a template, wherein the one or more second polymerization reactions are performed after measuring the third signal;
comparing the third signal and the fourth signal to determine the second change in signal.