Patent ID: 11963997
Assignee: JILIN UNIVERSITY
Field: Pharmaceuticals (Chemistry)
Classification: CPC A | IPC A

Claim 4:
5. The method according to claim 2, wherein the block copolymer RGD-PHPMA-b-Poly(MMA-alt-(Rhob-MA)) in step 1) is prepared as follows:
1) reducing rhodamine B to synthesize reduced rhodamine B, and functional modifying the reduced rhodamine B with methacryloyl chloride to obtain methacryloyl-functionalized rhodamine B (“Rhob-MA”);
2) subjecting methyl methacrylate (“MMA”), 4-cyanopentanoic acid dithiobenzoate (“CPADB”), azobisisobutyronitrile (“AIBN”), and the Rhob-MA to a reaction under N2 protection and using THF as a reaction solvent at 70° C. for 6 h after three times of freeze-thawing and degassing to obtain a product I, precipitating the product I with petroleum ether to obtain a precipitate I, and vacuum-drying the precipitate I to obtain a purified product polymethacryloyl rhodamine B-polymethyl methacrylate (“Poly(MMA-alt-(Rhob-MA))”);
3) subjecting hydroxypropyl methacrylate (“HPMA”), the AIBN, and the Poly(MMA-alt-(Rhob-MA)) to a reaction under N2 protection and by using THF as a reaction solvent at 70° C. for 12 h after three times of freeze-thawing and degassing to obtain a product II, precipitating the product II with diethyl ether to obtain a precipitate II, and vacuum-drying the precipitate II to obtain a purified product PHPMA-b-Poly(MMA-alt-(Rhob-MA));
4) subjecting N-hydroxy succinimide (“NHS”), 1-ethyl-3-(−3-dimethylaminopropyl) carbodiimide hydrochloride (“EDC”), and the PHPMA-b-Poly(MMA-alt-(Rhob-MA)) to a reaction with a 2-(N-morpholino)ethanesulfonic acid (“MES”) buffer as a reaction solvent at 20° C. to 25° C. for 24 h to obtain a product III; drying the product Ill and filtering with THF, and collecting a filtrate to obtain a purified product NHS-PHPMA-b-Poly(MMA-alt-(Rhob-MA)); and
5) subjecting the NHS-PHPMA-b-Poly(MMA-alt-(Rhob-MA)) and arginyl glycyl aspartic acid (“RGDfK”) to a reaction with an MES buffer as a reaction solvent at 20° C. to 25° C. for 24 h to obtain a product IV; dialyzing and purifying the product IV by dialysis to obtain a purified product solution, and freeze-drying the purified product solution to obtain purified RGD-PHPMA-b-Poly(MMA-alt-(Rhob-MA)).