Patent ID: 11891645
Assignee: NOVOZYMES A/S
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 13:
14. The variant of claim 10, comprising at least one substitution selected from the group consisting of G20L, G20R, S29A, S29C, S29N, S29P, A34N, A34R, A46E, S50L, F55R, I59M, S60E, S60P, S61P, S62N, S66M, D76A, D76G, D76K, D76L, D76N, D76P, D76V, D76Y, E81S, N87F, L96F, L96S, L96T, T98L, P101F, P101L, P101M, P101R, T102C, T102I, T103V, D109P, N115A, N115D, N115G, E117D, E117F, E117G, E117L, E117N, E117P, E117W, E117Y, N124I, N124K, N124V, F125L, F125M, L127P, G128N, G128S, G128W, G128Y, S130D, S130F, G141A, G141F, G141L, G141M, G141Q, G141R, Q142A, Q142L, Q142M, Q142S, Q142W, N145G, N145I, N145S, N145W, T146A, T146H, T146N, T146V, T146W, Q154R, Y159H, Y159S, T167D, Q182F, F199L, G208H, G208Y, V209F, V209G, V209H, V209L, V209N, V209Y, S210L, T213I, I228L, I228Q, I228Y, Y231F, Y231S, S237F, S237I, S237K, S237L, G244N, V261A, V266R, D267S, S272L, S272T, S272Y, Q275K, Q275R, T276F, T276V, I277L, D280F, D280N, D280S, D280T, A285S, S294T, G304H, G304K, G304P, G304R, G304S, K305H, K305I, L308P, F310M, F310P, S317C, S317G, S317H, S317I, S317K, S317L, S317R, S317W, S317Y, A319F, K321S, D326P, V327C, S336R, N338H, P341I, P341N, P341R, G345K, G345S, P348G, P348S, N356Y, A358L, L361P, T362I, and V364M, wherein the substitution at the one or more positions provides a protease variant having an increase in specific activity measured as improvement factor, AIF, of at least 2.6.