Patent ID: 11932901
Assignee: BECTON, DICKINSON AND COMPANY
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 0:
1. A method of labeling nucleic acid targets in a sample, comprising:
contacting copies of a plurality of nucleic acid targets with a plurality of oligonucleotide barcodes, wherein each oligonucleotide barcode comprises a first universal sequence, a target-binding region capable of hybridizing to the copies of the nucleic acid targets, and a molecular label;
extending the plurality of oligonucleotide barcodes hybridized to the copies of a plurality of nucleic acid targets to generate a plurality of first strand barcoded polynucleotides;
contacting random primers with the plurality of first strand barcoded polynucleotides, wherein each of the random primers comprises a second universal sequence, or a complement thereof,
extending the random primers hybridized to the plurality of first strand barcoded polynucleotides to generate a plurality of extension products, wherein the plurality of extension products comprises a plurality of target nucleic acid species;
providing a plurality of capture oligonucleotides capable of specifically binding to the plurality of target nucleic acid species; and
isolating capture oligonucleotides hybridized to the plurality of target nucleic acid species to generate an isolated plurality of target nucleic acid species.