Patent ID: 11858964
Assignee: VERIMMUNE INC.
Field: Pharmaceuticals (Chemistry)
Classification: CPC C  A  Y | IPC A  C

Claim 20:
21. A process for producing the composition of claim 1, which comprises:
(a) transforming a prokaryotic cell with an expression vector encoding the L1 protein;
(b) culturing the transformed prokaryotic cell under conditions that promote expression of the L1 protein;
(c) lysing the transformed prokaryotic cells to release expressed L1 protein;
(d) separating cell debris from the expressed L1 protein and recovering the L1 protein as inclusion bodies;
(e) optionally washing the L1 protein inclusion bodies;
(f) solubilizing the L1 protein inclusion bodies;
(g) refolding the L1 protein; and
(h) forming the icosahedron or dodecahedron capsid having a triangulation number T equal to 1 by incubating the refolded L1 protein in refolding buffer.