Patent ID: 11946087
Assignee: AMYRIS BIO PRODUCTS PORTUGAL, UNIPESSOAL, LDA
Field: Biotechnology (Chemistry)
Classification: CPC C  A | IPC A  C

Claim 36:
37. A host cell comprising:
(a) a heterologous nucleic acid encoding a phytoene synthase;
(b) a heterologous nucleic acid encoding a phytoene desaturase;, or comprising:
(a) at least one of:
(i) a heterologous nucleic acid encoding a phytoene synthase;
(ii) a heterologous nucleic acid encoding a lycopene cyclase; or
(iii) a heterologous nucleic acid encoding a bifunctional enzyme having phytoene synthase and lycopene cyclase activities; and

(b) a heterologous nucleic acid encoding a phytoene desaturase;, or comprising:
(a) at least one of:
(i) a heterologous nucleic acid encoding a phytoene synthase;
(ii) a heterologous nucleic acid encoding a lycopene cyclase; or
(iii) a heterologous nucleic acid encoding a bifunctional enzyme having phytoene synthase and lycopene cyclase activities;

(b) a heterologous nucleic acid encoding a phytoene desaturase; and
(c) a heterologous nucleic acid encoding a β-carotene ketolase;, or comprising:
(a) at least one of:
(i) a heterologous nucleic acid encoding a phytoene synthase;
(ii) a heterologous nucleic acid encoding a lycopene cyclase; or
(iii) a heterologous nucleic acid encoding a bifunctional enzyme having phytoene synthase and lycopene cyclase activities;

(b) a heterologous nucleic acid encoding a phytoene desaturase; and
(c) a heterologous nucleic acid encoding β-carotene hydroxylase;, or comprising:
(a) at least one of:
(i) a heterologous nucleic acid encoding a phytoene synthase;
(ii) a heterologous nucleic acid encoding a lycopene cyclase; or
(iii) a heterologous nucleic acid encoding a bifunctional enzyme having phytoene synthase and lycopene cyclase activities;

(b) a heterologous nucleic acid encoding a phytoene desaturase;
(c) at least one of:
(i) a heterologous nucleic acid encoding a β-carotene ketolase;
(ii) a heterologous nucleic acid encoding a β-carotene hydroxylase;
(iii) a heterologous nucleic acid encoding a cytochrome p450 hydroxylase and ketolase capable of converting β-carotene to echinenone and subsequently to β-cryptoxanthin and to astaxanthin; or
(iv) a heterologous nucleic acid encoding a cytochrome p450 reductase

which interacts with the cytochrome p450 hydroxylase and ketolase;, or comprising:
(a) a heterologous nucleic acid encoding a lycopene cyclase;
(b) a heterologous nucleic acid encoding a δ-carotene β-cyclase;
(c) a heterologous nucleic acid encoding β-ring hydroxylase; and
(d) a heterologous nucleic acid encoding a carotene ε-monooxygenase;, the host cell optionally further comprising:
a heterologous nucleic acid encoding a polyprenyl synthase for producing a polyprenyl diphosphate; or
a heterologous nucleic acid encoding a FPP synthase, or a heterologous nucleic acid encoding a GGPP synthase, or both; or
one or more heterologous nucleic acids encoding one or more or all of the enzymes of the mevalonte pathway.