Patent ID: 11951210
Assignee: THE JOHNS HOPKINS UNIVERSITY
Field: Pharmaceuticals (Chemistry)
Classification: CPC A | IPC A

Claim 0:
1. A giant unilamellar vesicle (GUV) comprising a unilamellar lipid bilayer, wherein the unilamellar lipid bilayer includes the outer surface of the GUV and encloses the inner lumen of the GUV;
wherein the unilamellar lipid bilayer comprises at least a first lipid and second lipid selected from a phosphatidylcholine (PC), phosphatidylserine (PS), phosphatidylglycerol (PG), phosphatidylinositol 4,5-biphosphate (PIP2), and DGS-NTA (Ni), and combinations thereof wherein the first and second lipid and combinations thereof may be the same or different;
and wherein the GUV comprises
a chemically inducible dimerization protein in the inner lumen and a binding partner protein on the outer surface; wherein the binding partner protein on the outer surface is fused with myristoylated alanine-rich protein kinase C substrate (MARCKS) or a C2 domain of lactadherin (lact-C2).