Patent ID: 11971408
Assignee: CLEVELAND DIAGNOSTICS, INC.
Field: Measurement (Instruments)
Classification: CPC G | IPC G

Claim 0:
1. A method for determining a risk level of cancer in a subject having an unknown type of cancer, the method comprising:
providing a biological fluid sample taken from the subject, wherein the sample comprises at least a first biomolecular species and a second biomolecular species;
partitioning the sample in an aqueous multi-phase system, wherein the first biomolecular species and the second biomolecular species partition differently in the aqueous multi-phase system, and the first biomolecular species and the second biomolecular species are each soluble in at least one phase of the aqueous multi-phase system;
determining partition coefficients for the first biomolecular species and the second biomolecular species in at least a first phase and a second phase of the aqueous multi-phase system, using at least a first assay specific to the first biomolecular species to determine the first biomolecular species and a second assay specific to the second biomolecular species to determine the second biomolecular species, wherein the second phase is substantially immiscible with the first phase at equilibrium;
comparing the partition coefficients of the first biomolecular species and the second biomolecular species with reference values to determine the presence or risk level of said unknown type of cancer in said subject based on a significant difference between the partition coefficients and the reference values, wherein the reference values are from individuals with and without cancer;
determining a type of cancer within the subject having the unknown type of cancer based on the comparing step;
selecting an anti-cancer drug or an anti-cancer therapeutic intervention for the subject based on the partition coefficients; and
treating the subject for said cancer with the anti-cancer drug or the anti-cancer therapeutic intervention.