Patent ID: 11866749
Assignee: DANMARKS TEKNISKE UNIVERSITET
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 11:
12. The recombinant microbial cell of claim 11, wherein the GCH1 is an E. coli GCH1 variant having at least 94% sequence identity to native E. coli GCH1 having the sequence of SEQ ID NO:16 and comprising an amino acid substitution selected from T198I, T198S, T198V, T198L, V179A, M99I, F214S and L200P, or a combination of amino acid substitutions selected from (a) to (n):
(a) I67V, T117I, A125D, H221R;
(b) E62K, N170K, L215P;
(c) V102M, L215P;
(d) Q157L, H212R;
(e) V28L, L215P, N222I;
(f) T108N, I133F, E213K;
(g) S5C, D57V, L215Q;
(h) H29Y, I75V, V179M;
(i) A14V, E46D, M61I, D97V;
(j) V28A, G42D, E213K;
(k) N52K, A68S, S207R;
(l) A41G, K129N, I133F;
(m) S3L, K184R, S199Y; and
(n) H12R, N170D, G187S.