Patent ID: 11959145
Assignee: HANNELORE BREITENBACH-KOLLER
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 0:
1. A screening system for a compound that ameliorates or reverts a defective or undesired translational read-through of a target gene bearing a premature termination codon (PTC) in a eukaryotic cell, the screening system comprising a recombinant eukaryotic cell
a) expressing a recombinant ribosome comprising a ribosomal protein having a deletion or other mutation that causes a defective or undesired translational read-through of a PTC-bearing target gene,
b) expressing a first reporter construct comprising said PTC-bearing target gene coupled to a gene encoding a first reporter group that is co-expressed with said PTC-bearing target gene,
c) expressing a second reporter construct comprising a gene encoding a second reporter group that is expressed independently from said PTC-bearing target gene as an internal standard,
wherein the first reporter construct and the second reporter construct are both expression cassettes or both expression vectors,
wherein said first and second reporter groups are independently selected from fluorescent protein reporter molecules, bioluminescent protein reporter molecules, maltose binding protein, phosphatase, peroxidase, kinase, chloramphenicol transferase, and β-galactosidase,
wherein said PTC-bearing target gene is selected from the group consisting of a LAMB3 gene and a CYH2 gene, and
wherein said ribosomal protein is selected from the group consisting of rPL19A, rPL25, and rPL10.