Patent ID: 11913027
Assignee: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA
Field: Pharmaceuticals (Chemistry)
Classification: CPC C  A | IPC A  C

Claim 17:
18. A method of treating Parkinson's disease in a subject, the method, comprising:
(i) isolating pluripotent stem cells (PASCs) by a method comprising:
(a) releasing adipose cells, including an adipocyte fraction and a stromal vascular fraction, from an adipose tissue sample using a proteolytic enzyme that breaks the peptide bonds in collagen;
(b) co-incubating the released adipocytes and the released stromal vascular fraction for 2-36 hours, wherein 4-24 hours of said co-incubation takes place under stress conditions in a medium containing a proteolytic enzyme, in the absence of nutrients, under hypoxic conditions, and decreasing the temperature to 4° C.;
(c) recovering viable cells following the co-incubation in step (b) by centrifuging to produce a cell pellet, removing supernatant containing adipocyte cell debris by aspiration, washing the cell pellet to remove the proteolytic enzyme, and resuspending the recovered cells in media wherein the recovered cells comprise PASCs; and

(ii) administering a composition comprising the PASCs to the subject under conditions permitting the PASCs of the composition to divide and populate a site of tissue damage.