Patent ID: 11883475
Assignee: SANARIA INC.
Field: Pharmaceuticals (Chemistry)
Classification: CPC A  C  Y | IPC A  C

Claim 16:
17. A method of culturing genetically attenuated Plasmodium parasites of human host range in vitro during sporogonic development of said parasites, comprising:
a. culturing the genetically attenuated Plasmodium parasites of human host range, which are at the gametocyte stage, to zygote stage in the presence of red blood cells in an exflagellation culture medium;
b. collecting a mixture comprising zygotes, gametes, gametocytes;
c. culturing said mixture on a substrate in an ookinete culture medium, wherein the genetically attenuated Plasmodium parasites of human host range differentiate to ookinetes;
d. exchanging said ookinete medium with an oocyst medium;
e. culturing the genetically attenuated Plasmodium parasites of human host range in the oocyst medium, wherein the genetically attenuated Plasmodium parasites of human host range differentiate to mature sporozoites; and
f. harvesting the genetically attenuated Plasmodium parasites of human host range produced thereby, wherein said genetically attenuated Plasmodium parasites of human host range are at a mature sporozoite stage.