Patent ID: 11913049
Assignee: RAMON GONZALEZ
Field: Biotechnology (Chemistry)
Classification: CPC C  Y | IPC C

Claim 9:
10. The method of claim 1, wherein said enzymes catalyzing the sequence of reactions for the generation of the product precursor acetyl-CoA comprises:
a. an overexpressed acyl-CoA dehydrogenase that catalyzes the conversion of said acyl-CoA to a transenoyl-CoA;
b. an overexpressed enoyl-CoA hydratase that catalyzes the hydration of said transenoyl-CoA to a 3-hydroxyacyl-CoA;
c. an overexpressed 3-hydroxyacyl-CoA dehydrogenase that catalyzes the oxidation of said 3-hydroxyacyl-CoA to a ß-ketoacyl-CoA; and
d. an overexpressed thiolase that catalyzes the cleavage of the acetyl-CoA from said ß-ketoacyl-CoA to produce the acetyl-CoA and an acyl-CoA 2-carbons shorter than said starting acyl-CoA in step a.