Patent ID: 11891632
Assignee: GENECAST CO., LTD
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 1:
2. The Taq polymerase mutant of claim 1, wherein the Taq polymerase mutant is used for discriminating a matched primer from a mismatched primer by comparing a Ct value of a quantitative polymerase chain reaction (qPCR) in the presence of a nucleic acid template and the matched primer with a Ct value of a qPCR in the presence of the nucleic acid template and the mismatched primer, wherein both the matched primer and the mismatched primer are capable of hybridizing with a target sequence of the nucleic acid template, and the mismatched primer has a non-complementary nucleotide at its 3′ end after it hybridizes to the target sequence of the nucleic acid template;
wherein the matched primer consisting of a nucleotide sequence of SEQ ID NO: 26, the mismatched primer consisting of a nucleotide sequence of SEQ ID NO: 27, and the nucleic acid template is a nucleic acid comprising a single nucleotide polymorphisms (SNP) rs1408799; or
wherein the matched primer consisting of a nucleotide sequence of SEQ ID NO: 29, the mismatched primer comprises a nucleotide sequence of SEQ ID NO: 30, and the nucleic acid template is a nucleotide acid comprising a SNP rs1015362; or
wherein the matched primer consisting of a nucleotide sequence of SEQ ID NO: 31, the mismatched primer consisting of a nucleotide sequence of SEQ ID NO: 32, and the nucleic acid template is a nucleotide acid comprising a SNP rs4911414.