Patent ID: 11933780
Assignee: FUNCTIONAL FLUIDICS INC.
Field: Measurement (Instruments)
Classification: CPC G  C | IPC C  G

Claim 18:
19. A method for assessing an effect on a cellular unit of an organism, comprising the steps of:
providing a first aliquot of a first sample including a first cellular unit not subject to the effect in a first supporting medium in a first hermetically sealed container;
providing a first aliquot of a second sample including a second cellular unit subject to the effect in a second supporting medium in a second hermetically sealed container;
deoxygenating the first supporting medium and the second supporting medium to induce at least one of a first predetermined amount of hypoxia and a first predetermined rate of oxygen consumption in the first supporting medium and in the second supporting medium;
determining, during deoxygenation of the first supporting medium, a value for at least one variable associated with the first cellular unit at each of a first plurality of different times;
determining, during deoxygenation of the second supporting medium, a value for at least one variable associated with the second cellular unit at each of a second plurality of different times;
providing a second aliquot of the first sample including a third cellular unit not subject to the effect in a third supporting medium in a third hermetically sealed container;
providing a second aliquot of the second sample including a fourth cellular unit subject to the effect in a fourth supporting medium in a fourth hermetically sealed container;
deoxygenating the third supporting medium and the fourth supporting medium to induce at least one of a second predetermined amount of hypoxia and a second predetermined rate of oxygen consumption in the third supporting medium and in the fourth supporting medium;
determining, during deoxygenation of the third supporting medium, a value for at least one variable associated with the third cellular unit at each of a third plurality of different times;
determining, during deoxygenation of the fourth supporting medium, a value for at least one variable associated with the fourth cellular unit at each of a fourth plurality of different times;
correlating the at least one of the first predetermined amount of hypoxia and the first predetermined rate of oxygen consumption with the values for the at least one variable associated with the first cellular unit and the first plurality of different times and correlating the at least one of the second predetermined amount of hypoxia and the second predetermined rate of oxygen consumption with the values for the at least one variable associated with the third cellular unit and the third plurality of different times to generate a first multi-dimensional surface;
correlating the at least one of the first predetermined amount of hypoxia and the first predetermined rate of oxygen consumption with the values for the at least one variable associated with the second cellular unit and the second plurality of different times and correlating the at least one of the second predetermined amount of hypoxia and the second predetermined rate of oxygen consumption with the values for the at least one variable associated with the fourth cellular unit and the fourth plurality of different times to generate a second multi-dimensional surface;
identifying differences between the first multi-dimensional surface and the second multi-dimensional surface.