Patent ID: 11932897
Assignee: UNIVERSITY OF KENTUCKY RESEARCH FOUNDATION
Field: Pharmaceuticals (Chemistry)
Classification: CPC C  A | IPC A  C

Claim 0:
1. A method of making a curcuminoid in a mammalian cell, the method comprising:
expressing enzymes 4-coumaroyl-CoA ligase (4CL1) and one of 1) curcuminoid synthase (CUS) and 2) curcumin synthase (CURS1) and diketide-CoA synthase (DCS) in the mammalian cell, and optionally further expressing in the mammalian cell one or more additional enzymes selected from the group consisting of tyrosine ammonia lyase (TAL), DCS, CURS1, 4 coumarate 3- hydroxylase (C3H), caffeoyl-CoA 3-O-methyltransferase (CCoAOMT), and acetyl-CoA carboxylase (ACC); and
contacting the mammalian cell expressing the enzymes with a starting material being tyrosine, ferulic acid or p-coumaric acid to convert the starting material to the curcuminoid.