Patent ID: 11926877
Assignee: THE BOARD OF TRUSTEES OF THE UNIVERSITY OF ILLINOIS
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 10:
11. A method of detecting viral polynucleotides in a saliva sample comprising:
combining the saliva sample with Tris Borate Ethylenediaminetetraacetic acid (TBE) at about a 1:3 w/w to about 3:1 w/w ratio prior to heating the saliva sample, to form a mixture;
heating the mixture at about 95 degrees Celsius for about 15 minutes to about 30 minutes;
after heating, contacting the heated mixture with one or more non-ionic detergents, wherein the one or more non-ionic detergents are present in a final concentration of about 0.25% to about 1% by weight to provide a test sample; and
subjecting the test sample to conditions that amplify target viral polynucleotides in the test sample using RT-qPCR, and detecting the viral polynucleotides in the biological sample.