Patent ID: 11944646
Assignee: AVOTRES, INC.
Field: Pharmaceuticals (Chemistry)
Classification: CPC A  C  G | IPC A  C  G

Claim 8:
9. The method of claim 8, wherein step (c) comprises:
(iv) activating CD8+ cells freshly isolated from the human individual with the prepared autologous immature dendritic cells of step (b);
(v) quantifying proliferation of specific target cells in a first cell population in the presence of the activated CD8+ cells of step (iv), wherein the specific target cells are from the cell line having the ATCC accession number PTA-127256, and the first cell population comprises an equal number of the specific target cells and B721 cells; and
(vi) quantifying proliferation of control target cells in a second cell population in the presence of the activated CD8+ cells of step (iv), wherein the control target cells are from the cell line having the ATCC accession number PTA-127257, and the second cell population comprises an equal number of the control target cells and B721 cells,
wherein a difference of more than 50% between the proliferation of the specific target cells of (v) and the proliferation of the control target cells of (vi) indicates that the prepared autologous immature dendritic cells of step (b) have a potency of at least 50%.