Patent ID: 11884960
Assignee: TERRAGENE SA
Field: Measurement (Instruments)
Classification: CPC C  A  G | IPC C  G

Claim 0:
1. A biological indicator for determining the efficacy of a steam or heat sterilization process, wherein said biological indicator comprises, in a single container,
a set of microbial spores,
at least one protein exogenous to the microbial spores comprising an enzyme,
a fluorophore, and
a culture medium, comprising a colorimetric component,, wherein the fluorophore is capable of interacting with the at least one protein exogenous to the microbial spores, said interaction yielding an immediate optically detectable signal independent of a catalytic activity of the enzyme, and, wherein after the steam or heat sterilization process the culture medium contacts the microbial spores and produces an optically detectable color shift in the presence of microbial growth.