Patent ID: 11866793
Assignee: REGENTS OF THE UNIVERSITY OF MINNESOTA
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 15:
16. A kit for determining relative abundance of Amaranthus palmeri DNA in a sample, the kit comprising:
a. a first primer that recognizes a first locus in the genome of Amaranthus and is specific to the sequence variant found in Amaranthus palmeri, 
b. a second primer that recognizes the first locus and is specific to the sequence variant found in all Amaranthus species other than Amaranthus palmeri, and
c. a third primer that recognizes a second locus in the genome of Amaranthus and is specific to a sequence found in all Amaranthus species,
d. a first heterologous reporter molecule, and
e. a second heterologous reporter molecule,
wherein the first primer comprises SEQ ID NO: 1 and the second primer comprises SEQ ID NO: 2 or the first primer comprises SEQ ID NO: 4 and the second primer comprises SEQ ID NO: 5; and
wherein the first primer further comprises a first heterologous reporter sequence that is complementary in sequence to the first heterologous reporter molecule, and the second primer further comprises a second heterologous reporter sequence that is complementary in sequence to the second heterologous reporter molecule; and
wherein the first heterologous reporter sequence comprises one of SEQ ID NO: 10 or SEQ ID NO: 11 and the second heterologous reporter sequence comprises the other of SEQ ID NO: 10 or SEQ ID NO: 11.