Patent ID: 11873499
Assignee: INSTITUTE OF GENETICS AND DEVELOPMENTAL BIOLOGY, CHINESE ACADEMY OF SCIENCES
Field: Biotechnology (Chemistry)
Classification: CPC C  A  Y | IPC C

Claim 0:
1. A genetically altered plant, part thereof or plant cell, wherein the plant is characterised by an increase in at least one of nitrogen uptake and/or nitrogen assimilation and/or nitrogen use efficiency compared to a control plant, wherein
a) the genetically altered plant, part thereof or plant cell comprises a nucleic acid construct comprising a GRF4 nucleic acid, wherein the GRF4 nucleic acid encodes a GRF polypeptide comprising SEQ ID NO: 3 or 6; or
b) the genetically altered plant, part thereof or plant cell comprises at least one mutation in a GRF4 promoter operably linked to a gene encoding SEQ ID NO: 3 or 6, wherein the GRF4 promoter comprises SEQ ID NO: 7 or 8; and wherein the mutation is a T to A substitution at position −884 or −878 from the GRF4 start codon ATG of an open reading frame for a polypeptide with SEQ ID NO: 3 or 6; and a C to T substitution at position 847 or −841 from the GRF4 start codon ATG of the open reading frame for the polypeptide with SEQ ID NO: 3 or 6; and a C to T substitution at position-801 or −795 from the GRF4 start codon ATG of the open reading frame for the polypeptide with SEQ ID NO: 3 or 6,
and wherein the control plant does not comprise the nucleic acid construct of a) or the at least one mutation of b).