Patent ID: 11965178
Assignee: CELLPHIRE, INC.
Field: Pharmaceuticals (Chemistry)
Classification: CPC C  A | IPC A  C

Claim 0:
1. A method of preparing chemotherapy drug-loaded, freeze-dried platelet derivatives, comprising:
a) providing platelets;
b) treating the platelets with a rubicin selected from the group consisting of daunorubicin, doxorubicin, epirubicin, idarubicin, and valrubicin in the presence of a loading buffer comprising a salt, a base, and a loading agent comprising trehalose in an amount of 10 mM to 500 mM to form chemotherapy drug-loaded platelets, wherein the chemotherapy drug-loaded platelets comprise 1 nM to 100 mM of the rubicin; and
c) lyophilizing the chemotherapy drug-loaded platelets to form chemotherapy drug- loaded, freeze-dried platelet derivatives, having the property of retaining at least 20% of the rubicin upon rehydration, having less than 10% crosslinking of platelet membranes via proteins and/or lipids present on the platelet membranes, and having the property of inhibiting cancer cell growth more effectively than the rubicin alone.