Patent ID: 11896658
Assignee: THE BOARD OF REGENTS FOR OKLAHOMA STATE UNIVERSITY
Field: Pharmaceuticals (Chemistry)
Classification: CPC A  C | IPC A  C

Claim 8:
9. A mammalian cell, comprising:
at least one polynucleotide encoding a baculovirus GP64 envelope glycoprotein or variant or fragment thereof; and
at least one polynucleotide encoding at least a portion of a genome of an infection-attenuated virus of the Pneumoviridae family, wherein the genome comprises a gene encoding an RSV F protein variant or fragment thereof that comprises at least one amino acid substitution compared to a native RSV F protein, wherein the at least one amino acid substitution stabilizes the RSV F protein variant or fragment thereof in a pre-fusion conformation, and wherein the genome comprises at least one additional modification that renders the virus infection-attenuated; and
wherein the cell produces a recombinant, live, attenuated virus of the Pneumoviridae family.