Patent ID: 11857628
Assignee: MOLECULAR TEMPLATES, INC.
Field: Pharmaceuticals (Chemistry)
Classification: CPC A  C | IPC A  C

Claim 0:
1. A cell-targeting molecule comprising:
(a) a Shiga toxin effector polypeptide;
(b) a binding region capable of specifically binding at least one extracellular target biomolecule; and
(c) a linker peptide between the Shiga toxin effector polypeptide and the binding region,
wherein the Shiga toxin effector polypeptide comprises an amino acid sequence having at least 90% sequence identity to amino acids 1 to 251 of SEQ ID NO: 1,
wherein the Shiga toxin effector polypeptide comprises amino acid substitutions S45I, V54I, R55L, I57F, P59F, E60T, E61L, G110A, and R188A in SEQ ID NO: 1,
wherein the Shiga toxin effector polypeptide comprises one or more cysteines suitable for conjugation to a heterologous molecule, and wherein the Shiga toxin effector polypeptide comprises at least one of the following amino acid substitutions in SEQ ID NO: 1: S8C, S16C, S22C, S43C, S146C, and S186C.