Patent ID: 11970747
Assignee: BEIJING ORIGINPOLY BIO-TEC CO., LTD.
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 0:
1. A primer-probe combination for vaginal microecosystem detection suitable for high-resolution melting curve analysis, comprising primers and probes for target gene detection against Lactobacillus crispatus (LC), Lactobacillus gasseri (LG), Lactobacillus jensenii (LJ), Lactobacillus iners (LI), Gardnerella vaginalis (GV), Candida albicans (CA) and Trichomonas vaginalis (TV), with nucleotide sequences shown below:
Lactobacillus crispatus detection primer LC-F: SEQ ID NO: 1,
Lactobacillus crispatus detection primer LC-R: SEQ ID NO: 2,
Lactobacillus crispatus detection probe LC-FP: SEQ ID NO: 3;
Lactobacillus gasseri detection primer LG-F: SEQ ID NO: 4,
Lactobacillus gasseri detection primer LG-R: SEQ ID NO: 5,
Lactobacillus gasseri detection probe LG-FP: SEQ ID NO: 6;
Lactobacillus jensenii detection primer LJ-F: SEQ ID NO: 7,
Lactobacillus jensenii detection primer LJ-R: SEQ ID NO: 8,
Lactobacillus jensenii detection probe LJ-FP: SEQ ID NO: 9;
Lactobacillus iners detection primer LI-F: SEQ ID NO: 10,
Lactobacillus iners detection primer LI-R: SEQ ID NO: 11,
Lactobacillus iners detection probe LI-FP: SEQ ID NO: 12;
Gardnerella vaginalis detection primer GV-F: SEQ ID NO: 13,
Gardnerella vaginalis detection primer GV-R: SEQ ID NO: 14,
Gardnerella vaginalis detection probe GV-FP: SEQ ID NO: 15;
Candida albicans detection primer CA-F: SEQ ID NO: 16,
Candida albicans detection primer CA-R: SEQ ID NO: 17,
Candida albicans detection probe CA-FP: SEQ ID NO: 18; and
Trichomonas vaginalis detection primer TV-F: SEQ ID NO: 19,
Trichomonas vaginalis detection primer TV-R: SEQ ID NO: 20,
Trichomonas vaginalis detection probe TV-FP: SEQ ID NO: 21;
wherein the nucleotide sequences of the probes are labeled with fluorophores at the 5′ end and quenching groups at the 3′ end.