Patent ID: 11920174
Assignee: CUREVAC SE
Field: Biotechnology (Chemistry)
Classification: CPC C  G | IPC C  G

Claim 9:
10. The method of claim 9, wherein the measured ratio of a specific nucleoside in the sample is determined using formula 2.1:

rm(X)=n(X)/[n(C)+n(G)+n(U)], and the expected ratio of said nucleoside is determined using formula 3.1:

re(X)=u(X)/[u(C)+u(G)+u(U)],, wherein rm is the measured ratio of a specific nucleoside in the sample, re is the expected ratio of the corresponding nucleoside in the RNA molecules, n(C), n(U), and n(G) is the measured content of the corresponding nucleoside and any modifications thereof in the sample, u(C), u(U), and u(G) is the number of the corresponding nucleoside and any modifications thereof in the expected sequence of the RNA molecules, and X is a nucleotide selected from C, G and U and is the same nucleotide for both rm(X) and re(X), wherein a mismatch between the values for rm and re indicates that the sequence of the RNA molecules does not match with the expected sequence and/or that the length of the RNA molecules does not match with the expected length.