Patent ID: 11892444
Assignee: ROCHE SEQUENCING SOLUTIONS, INC.
Field: Measurement (Instruments)
Classification: CPC G  C  B | IPC B  C  G

Claim 10:
11. A method of calibrating a sequencing chip including a plurality of sequencing cells, each sequencing cell comprising a nanopore, the method comprising:
applying a first voltage across the nanopore of each sequencing cell while the nanopore is in an unthreaded state and measuring an open channel voltage for each sequencing cell;
inserting a molecule into nanopore of each sequencing cell so that the nanopore is in a threaded state;
applying a second voltage across the nanopore of each sequencing cell while the nanopore is in the threaded state and measuring a first voltage signal for each sequencing cell;
normalizing the first voltage signal for each sequencing cell with the corresponding open channel voltage for each sequencing cell; and
determining an identity of at least a portion of the molecule based on the normalized first voltage signal.