Patent ID: 11963979
Assignee: BAYLOR COLLEGE OF MEDICINE
Field: Pharmaceuticals (Chemistry)
Classification: CPC C  A  Y | IPC A  C

Claim 13:
14. An in vitro process for rapid expansion of antigen specific T cells, comprising culturing in a gas permeable vessel a population of PBMCs in a culture in the presence of an antigen, wherein the antigen is in the form of a peptide or a peptide mix relevant to a target antigen(s) and wherein the antigen is added to the culture in the range of from 1 to 500 ng per ml; wherein the culturing is performed in the presence of an exogenous cytokine wherein the cytokine is other than exogenous IL-2 and is selected from the group comprising IL-1, IL-4, IL-6, IL-7, IL-12, IL-15 and combinations thereof; wherein the process consists of expanding the antigen-specific T cells for 20, 18, 17, 16, 15, 14, 13, 12, 11, 10, 9, 8, 7 days or less wherein the desired population of cells is a CD56− population, and the peptide or peptide mix is/are not added to the culture presented on dendritic cells; and wherein antigens, cytokines, media and nutrients are not added or changed after initiation of the expansion process.