Patent ID: 11970550
Assignee: HOFFMANN-LA ROCHE, INC.
Field: Measurement (Instruments)
Classification: CPC C  G | IPC C  G

Claim 5:
6. A method of detecting in a biological fluid sample the presence of a therapeutic antibody of a IgG1 or IgG4 subclass comprising the variant Fc-region with the mutation P329G, L234A, and L235A or the mutations P329G, L234A, L235A, I253A, H310A and H435A, wherein the amino-acid positions are numbered according to Kabat EU index, the method comprising
(a) contacting the biological fluid sample with the antibody of claim 1, wherein the antibody of claim 1 specifically binds the therapeutic antibody of the IgG1 or IgG4 subclass comprising the variant Fc-region with the mutation P329G, L234A, and L235A or the mutations P329G, L234A, L235A, I253A, H310A and H435A, to form a complex; and
(b) detecting the complex, wherein detection of the complex correlates to the presence of the therapeutic antibody of the IgG1 or IgG4 subclass comprising the variant Fc-region with the mutation P329G, L234A, and L235A or the mutations P329G, L234A, L235A, I253A, H310A and H435A, wherein the antibody of claim 1 is either a capture or tracer antibody.