Patent ID: 11879127
Assignee: DANISCO US INC.
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 0:
1. A method for introducing a recombinant DNA construct into a modified Bacillus licheniformis host cell, the method comprising:
(a) providing a modified B. licheniformis host cell comprising an inactivated genomic diaminopimelate decarboxylase (lysA) gene,
(b) introducing into the modified cell a recombinant DNA construct comprising a selectable marker encoding a diaminopimelate decarboxylase (LysA) protein,
(c) cultivating the host cell of step (b) in a growth-medium which does not comprise lysine, and
(d) selecting a host cell comprising the DNA construct that is capable of growing in the medium of step (c).