Patent ID: 11905623
Assignee: PRESIDENT AND FELLOWS OF HARVARD COLLEGE
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 0:
1. A method for engineering a nucleic acid molecule, the method comprising contacting a first nucleic acid molecule comprising a first recombinase target sequence of the structure
5′-[left half-site]-[spacer]-[right half-site]-3′, with a recombinase and a second nucleic acid molecule comprising a second recombinase target sequence under conditions suitable for the recombinase to bind and recombine the recombinase target sequences, wherein the first and the second recombinase target sequences differ from the canonical Cre recombinase target sequence 5′-ATAACTTCGTATA GCATACAT TATACGAAGTTAT-3′ (SEQ ID NO: 2) in at least 1, at least 2, at least 3, at least 4, at least 5, at least 6, at least 7, at least 8, at least 9, at least 10, at least 11, at least 12, at least 13, at least 14, or at least 15 nucleotides.