Patent ID: 11944705
Assignee: MOOGENE MEDI CO., LTD.
Field: Pharmaceuticals (Chemistry)
Classification: CPC A  B | IPC A  B

Claim 0:
1. A method of preparing a nanoliposome-microbubble conjugate in which a drug for hair loss treatment is encapsulated in a nanoliposome, comprising:
1) preparing a nanoliposome by:
1A) preparing a lipid film composition by mixing (a) lecithin, (b) cholesterol and (c) a first phospholipid in chloroform which is at least one selected from the group consisting of dioleoyl phosphatidylethanolamine (DOPE), 1,2-diphytanoyl-sn-glycero-3-phosphoethanolamine (DPhPE), 1,2-distearoyl-sn-glycero-3-phosphoethanolamine (DSPE), and 1,2-dipalmitoyl-sn-glycero-3-phosphoethanolamine (DPPE);
1B) adding the lipid film composition with a drug solution for hair loss treatment and performing sonication, wherein the drug for hair loss treatment comprises at least one selected from the group consisting of finasteride, minoxidil, and dutasteride;
1C) subjecting the sonicated lipid film composition to freezing and thawing and then to sonication; and
1D) centrifuging the lipid film composition sonicated in step 3 and recovering a nanoliposome that is precipitated,
2) separately preparing a microbubble by:
2A) preparing a lipid film composition by mixing (i) a second phospholipid which is at least one selected from the group consisting of 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC), 1,2-distearoyl-sn-glycero-3-phosphocholine (DSPC), 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC), 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC), 1-myristoyl-2-palmitoyl-sn-glycero-3-phosphocholine (MPPC) and 1-myristoyl-2-stearoyl-sn-glycero-3-phosphocholine (MSPC), (ii) cholesterol, (iii) a third phospholipid which is at least one from the group consisting of selected from the group consisting of dicetyl phosphate (DCP), 1,2-dierucoyl-sn-glycero-3-phosphate (DEPA), 1,2-dilauroyl-sn-glycero-3-phosphate (DLPA), 1,2-dimyristoyl-sn-glycero-3-phosphate (DMPA) and 1,2-dioleoyl-sn-glycero-3-phosphate (DOPA), (iv) a fourth phospholipid which is at least one selected from the group consisting of dioleoyl phosphatidylethanolamine (DOPE), 1,2-diphytanoyl-sn-glycero-3-phosphoethanolamine (DPhPE), 1,2-distearoyl-sn-glycero-3-phosphoethanolamine (DSPE), and 1,2-dipalmitoyl-sn-glycero-3-phosphoethanolamine (DPPE) and (v) DSPE-PEG-sPDP (1,2-distearoyl-sn-glycero -3-phosphoethanolamine-N-polv(ethyleneglycol)-2000-N-[3-(2-pyridyldithio)propionate]in chloroform;
2B) adding a glucose solution to the lipid film composition and performing sonication;
2C) subjecting the sonicated lipid film composition comprising glucose to freezing and thawing and then to additional sonication; and
2D) introducing a hydrophobic gas into the lipid film composition that has been subjected to freeze-thaw and sonication to prepare a microbubble; and
3) mixing the nanoliposome with the microbubble to prepare a nanoliposome-microbubble conjugate.