Patent ID: 11970720
Assignee: SALK INSTITUTE FOR BIOLOGICAL STUDIES
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 0:
1. A non-naturally occurring or engineered clustered regularly interspaced short palindromic repeats (CRISPR)-associated (Cas) system for targeting one or more target RNA molecules, comprising:
(a) at least one catalytically inactive Cas13d protein comprising one or more mutated HEPN domains, or a nucleic acid molecule encoding the at least one catalytically inactive Cas13d protein; and
(b) at least one CRISPR-Cas system guide RNA (gRNA) comprising one or more direct repeat (DR) sequences and one or more spacer sequences, which hybridizes with the one or more target RNA molecules, or at least one nucleic acid molecule encoding the gRNA,
whereby the catalytically inactive Cas13d protein forms a complex with the gRNA, wherein the gRNA directs the complex to the one or more target RNA molecules and targets the one or more target RNA molecules,
wherein the at least one catalytically inactive Cas13d protein comprises a motif sequence of one or more of SEQ ID NOS: 195, 196, 197, 288, 289, 290, and 291, and,
wherein the one or more mutated HEPN domains comprise the amino acid sequence RXXXXH (SEQ ID NO: 255), and wherein the R, the H, or both the R and the H, of the amino acid sequence RXXXXH (SEQ ID NO: 255) is mutated.