Patent ID: 11898164
Assignee: S-BIOMEDIC NV
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 0:
1. A method for producing spherical neural masses having suppressed teratoma formation, the method including the following steps:
1) fragmentation of the spherical neural masses to obtain fragmented spherical neural masses;
2) adhesion-culturing of the fragmented spherical neural masses in a culturing dish comprising culturing medium for neuronal progenitor cell to obtain adhesion-cultured spherical neural masses;
3) performing single-cellularization of the adhesion-cultured spherical neural masses by adding enzyme to obtain single cells;
4) inducing spontaneous aggregation of cells by adhesion-culturing of the single cells of step 3) in a culturing dish without an additional substrate coated thereon and without adding a differentiation-inducing factor to obtain aggregates attached to a bottom of the culturing dish;
5) removing floating cells after step 4) and inducing spontaneous re-aggregation of single cells by repeatedly suspending the aggregates attached to the bottom of culturing dish to obtain re-aggregated spherical neural masses; and
6) recovering the spherical neural masses re-aggregated in step 5), wherein the re-aggregated spherical neural masses contain fewer undifferentiated cells compared to the spherical neural masses in step 1).