Patent ID: 11898961
Assignee: ALIO, INC.
Field: Measurement (Instruments)
Classification: CPC G  A  C | IPC G

Claim 11:
12. A method for evaluating analytes for periprosthetic joint infection (PJI) in a synovial fluid sample, the method comprising:
providing a vial receptacle sized to receive a vial containing a fluid sample;
providing one or more light sources oriented to illuminate the fluid sample in the vial;
providing a vial optical sensor oriented to detect light scattered, light absorbed, or both by white blood cells in the fluid sample, wherein the vial optical sensor is configured to generate a vial electrical signal indicative of an intensity of detected light;
providing at least one immunoassay strip receptacle sized to receive a leukocyte esterase (LE) immunoassay strip having an LE analyte reagent and a portion of the fluid sample,
wherein the LE analyte reagent is configured to generate an analyte light intensity from a reaction between the LE analyte reagent and an analyte in the fluid sample;
providing an immunoassay optical sensor oriented to detect the analyte light intensity and to convert the analyte light intensity to an LE immunoassay analyte electrical signal; and
providing a processor communicatively coupled to the vial optical sensor and the immunoassay strip optical sensor via a communications interface, the processor configured to (i) receive an indication of a presence of the vial containing fluid sample in a vial receptacle, (ii) provide an instruction to apply to the one or more light sources directed at the vial containing the fluid sample, (iii) provide an instruction to initiate the vial optical sensor (iv) receive the vial electrical signal from the vial optical sensor indicative of the intensity of detected light, (v) receive an indication of a presence of the LE immunoassay strip in the at least one immunoassay receptacle, (vi) provide an instruction to initiate the immunoassay optical sensor, (vii) receive LE immunoassay analyte electrical signal from the immunoassay optical sensor, and (viii) calculate a white blood cell (WBC) concentration and a leukocyte concentration from the vial electrical signal and the LE immunoassay analyte electrical signal.