Patent ID: 11912763
Assignee: L & L BIOPHARMA CO., LTD.
Field: Pharmaceuticals (Chemistry)
Classification: CPC A  C | IPC A  C

Claim 18:
19. The bispecific antibody as defined in claim 6, wherein the bispecific antibody is selected from any one of the following:
(1) the first protein functional region is an immunoglobulin, and the immunoglobulin comprises a light chain amino acid sequence as shown in SEQ ID NO: 38 and a heavy chain amino acid sequence as shown in SEQ ID NO: 39; the second protein functional region is scFv; wherein
the C-terminals of the heavy chain variable regions of the two scFvs are symmetrically linked to the N-terminals of the two heavy chains of the immunoglobulin via a linker; and, the light chain variable region of the scFv is the light chain variable region of Atezolumab, and the heavy chain variable region of the scFv is the heavy chain variable region of Atezolumab; or,
the C-terminals of the heavy chain variable regions of the two scFvs are symmetrically linked to the N-terminals of the two heavy chain variable regions of the immunoglobulin via a linker; and, the light chain variable region of the scFv is the light chain variable region of Hu5F9, and the heavy chain variable region of the scFv is the light chain variable region of Hu5F9; or,
the N-terminals of the heavy chain variable regions of the two scFvs are symmetrically linked to the C-terminals of the two heavy chains of the immunoglobulin via a linker; and, the light chain variable region of the scFv is the light chain variable region of AMG420, and the heavy chain variable region of the scFv is the heavy chain variable region of AMG420;
(2) the first protein functional region is scFv, and the second protein functional region is an immunoglobulin; the C-terminals of the heavy chain variable regions of the two scFvs are symmetrically linked to the N-terminals of the two heavy chains of the immunoglobulin via a linker; the sequence of the light chain variable region of the scFv is as shown in SEQ ID NO: 29, and the sequence of the heavy chain variable region of the scFv is as shown in SEQ ID NO: 34; wherein
the immunoglobulin comprises the amino acid sequences of the light chain variable region of Nivolumab, the light chain constant region κ chain, the heavy chain variable region of Nivolumab and the heavy chain constant region of hIgG4; or,
the immunoglobulin comprises the amino acid sequences of the light chain variable region of Pembrolizumab, the light chain constant region κ chain, the heavy chain variable region of Pembrolizumab and the heavy chain constant region of hIgG4; or,
the immunoglobulin comprises the amino acid sequences of the light chain variable region of Atezolumab, the light chain constant region κ chain, the heavy chain variable region of Atezolumab and the heavy chain constant region of hIgG1.