Patent ID: 11891645
Assignee: NOVOZYMES A/S
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 3:
4. The variant of claim 1, comprising at least one substitution selected from the group consisting of: S5K, S5L, S5Y, T9H, K25P, S29R, S30E, K32W, F38L, I39G, I39L, I39V, I39Y, D40F, D40G, D40H, D40N, D40P, Q41I, Q41V, F42C, F42K, F42S, F42Y, K45L, K45M, K45Y, A46C, K49P, S50I, S50L, S50M, S50Q, A53C, A53E, A53V, Q54I, Q54L, Q54M, Q54V, F55H, F55L, K57P, K57S, I59G, I59M, I59P, S60P, S61L, S61P, S62C, S62N, S62P, T64G, T64I, L67V, Q68V, Q68Y, T69C, E74R, D76L, D76R, Q77L, S78D, P79Y, S80L, E81A, E81H, E81K, E81L, E81P, E81Y, A82M, A82P, N87S, T93L, V94S, G99C, T102L, T102V, T103I, T103K, T103V, I105Q, D109H, D109K, D109M, D109Y, F111H, F111K, F111S, Q112R, G114L, N115C, N115K, N115R, N115T, N115Y, E117D, E117G, E117H, E117N, E117Q, E117S, I122R, N124L, G128F, G128S, S130G, S130N, S130P, L136K, G141T, Q142A, Q142F, Q142L, Q142N, N145V, T146A, T146L, T146N, I147S, K150L, N153M, N153P, N153R, Q154I, Q154L, Q154N, Q154T, Q154V, N157L, Q161E, T167F, 1169F, D175N, Q182C, Q182T, Q182V, F199L, F199M, M200S, A205S, Q207H, Q207L, V209F, S210T, T213R, F217I, V225Y, I228L, I228T, I228W, S234Q, S237F, G244S, S245P, T246S, S248Y, F258P, S262P, V266K, V266R, D267A, D267H, D267L, D267N, Q269L, V271R, S272K, S272L, S272R, S272V, S272Y, T276Y, G278P, D280N, D280S, D280T, C284G, F289Y, I293T, V296A, R299N, K305S, L308V, F314L, S317E, S317W, S318P, A319F, A319Q, K321L, K321Q, K321S, A322D, L324Q, L324S, D326P, S329C, S329H, S329I, S329L, S329N, S329W, G330Q, S331W, S331Y, S336K, S336N, P341S, K343A, K343H, K343S, K343T, G345S, D347I, P348Y, P355I, A358L, A358P, A358R, A358Y, K359L, K359S, L361M, T362L, A363G, A363L, and V364I, wherein the substitution at the one or more positions provides a protease variant having an increase in thermo-stability measured as improvement factor, HIF, of at least 1.6.