Patent ID: 11866745
Assignee: PAIRWISE PLANTS SERVICES, INC.
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 22:
23. A method of editing a target nucleic acid, comprising contacting a cell or a cell free system comprising the target nucleic acid with:
(a)(i) a polynucleotide encoding a fusion protein comprising the modified LbCas12a polypeptide of claim 19 and a polypeptide of interest, or an expression cassette or vector comprising the same, and (a)(ii) a guide nucleic acid, or an expression cassette or vector comprising the same; and/or
(b) a nucleic acid construct encoding a complex comprising a fusion protein comprising the modified LbCas12a polypeptide of claim 19 and a polypeptide of interest, and a guide nucleic acid, or an expression cassette or vector comprising the same; and/or
(c) a Type V CRISPR-Cas system comprising:
a guide nucleic acid comprising a spacer sequence and a repeat sequence, and (i) a fusion protein comprising the modified LbCas12a polypeptide of claim 19 and a polypeptide of interest, or (ii) a nucleic acid encoding the modified LbCas12a polypeptide of claim 19 and a nucleic acid encoding a polypeptide of interest,
wherein the guide nucleic acid is capable of forming a complex with the modified LbCas12a polypeptide or the fusion protein and the spacer sequence is capable of hybridizing to a target nucleic acid, thereby guiding the modified LbCas12a polypeptide and the polypeptide of interest to the target nucleic acid, thereby editing the target nucleic acid.