Patent ID: 11884950
Assignee: OXFORD NANOPORE TECHNOLOGIES PLC
Field: Biotechnology (Chemistry)
Classification: CPC C  B | IPC B  C

Claim 5:
6. The method according to claim 3, wherein in a given synthesis cycle the universal nucleotide occupies position n in:
(a) the support strand of the scaffold polynucleotide in steps (1) and (6), wherein position n is the nucleotide position in the support strand which is opposite the position in the synthesis strand which will be occupied by the nucleotide of the predefined sequence upon its incorporation in that cycle, and (b) the support strand of the ligation polynucleotide in steps (4) and (8) wherein position n is the nucleotide position in the support strand which is opposite the position in the synthesis strand which will be occupied by the next nucleotide of the predefined sequence upon its incorporation in the next synthesis cycle,
wherein position n−1 is the next nucleotide position in the support strand relative to the position occupied by the universal nucleotide in the direction distal to the helper strand and proximal to the primer strand, and wherein position n−2 is the next nucleotide position in the support strand relative to position n−1 in the direction distal to the helper strand and proximal to the primer strand; and
wherein the support strand of the scaffold polynucleotide is cleaved between positions n−1 and n−2 in steps (3) and (7).