Patent ID: 11965208
Assignee: BECTON, DICKINSON AND COMPANY
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 25:
26. The method of claim 22, further comprising:
stochastically labeling the unique cell indexing oligonucleotides associated with the partitioned plurality of cells using a plurality of the stochastic labels to generate a plurality of stochastically labeled cell indexing oligonucleotides, wherein each of the stochastic labels comprises a cellular label, wherein the stochastic labels associated with the same cell comprise the same cellular label, and wherein the stochastic labels associated with different cells comprise different cellular labels;
obtaining sequencing data of at least a portion of the cellular label and at least a portion of the cell indexing sequence associated with the same stochastically labeled unique cell indexing oligonucleotide; and
determining the distinct spatial location on the substrate to which a cell associated with the same stochastically labeled unique cell indexing oligonucleotide was partitioned, based on the at least a portion of the cellular label, the at least a portion of the cell indexing sequence, and the spatial index.