Patent ID: 11913016
Assignee: WISCONSIN ALUMNI RESEARCH FOUNDATION
Field: Biotechnology (Chemistry)
Classification: CPC C  Y | IPC C

Claim 0:
1. A method of making a synthetic polyploid yeast cell comprising:
i) introducing a first polynucleotide comprising a first promoter operably connected to an HO polynucleotide encoding a yeast Ho protein, a first selectable marker cassette, and a first yeast origin of replication into a first yeast cell from a first yeast hybrid to produce a first transformed yeast cell, wherein the first yeast hybrid and the first transformed yeast cell have a ploidy of at least 4N,
ii) introducing a second polynucleotide comprising a second promoter operably connected to an HO polynucleotide encoding a yeast Ho protein, a second selectable marker cassette, and a second yeast origin of replication into a second yeast cell from a second yeast species or hybrid to produce a second transformed yeast cell,
iii) co-culturing the first transformed yeast cell with the second transformed yeast cell to produce a mating mixture and allow for mating of the first transformed yeast cell and the second transformed yeast cell, and
iv) selecting at least a portion of the mating mixture for the first selectable marker cassette and the second selectable marker cassette to select for a synthetic polyploid yeast cell produced by the mating of step iii,
wherein the first selectable marker cassette and the second selectable marker cassette encode different selectable markers, wherein the first yeast cell and the second yeast cell are from different species of the same genus, and wherein the resulting synthetic polyploid yeast cell has a ploidy of at least 6N and comprises whole genomes from at least three different yeast species.