Patent ID: 11859236
Assignee: WENZHOU MEDICAL UNIVERSITY
Field: Measurement (Instruments)
Classification: CPC C  G  Y | IPC C

Claim 5:
6. The method for detecting L-serine based on cysteine desulfurase-containing Escherichia coli cells according to claim 1, wherein the cysteine desulfurase-containing living Escherichia coli cells are prepared by the following steps:
transforming a pBAD expression vector pBISCS containing cysteine desulfurase IscS into Escherichia coli MC4100 to construct a pBISCS/MC4100 strain;
inoculating the pBISCS/MC4100 strain into LB broth supplemented with ampicillin to obtain a bacterial suspension with an OD600 of 0.6-0.8, and inducing the bacterial suspension with L-arabinose for 2-3 h to harvest cells; and
washing the cells once or twice with M9 Buffer, and resuspending the cells in M9 Buffer supplemented with chloramphenicol and ampicillin to obtain the cysteine desulfurase-containing living Escherichia coli cells.