Patent ID: 11919928
Assignee: KUMIAI CHEMICAL INDUSTRY CO., LTD.
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 0:
1. A method for producing dihydrotentoxin, comprising the steps of:
culturing a transformant into which a tentoxin synthesis-related gene has been introduced, wherein the tentoxin synthesis-related gene encodes a protein having activity of nonribosomal peptide synthesis of dihydrotentoxin and containing the following modules in the order below from the N-terminal side:
a first module having a first adenylation domain comprising the amino acid sequence of SEQ ID NO: 1 or an amino acid sequence having at least 90% sequence identity with the amino acid sequence of SEQ ID NO: 1 with adenylation activity, a first peptidyl carrier protein domain comprising the amino acid sequence of SEQ ID NO: 2 or an amino acid sequence having at least 90% sequence identity with the amino acid sequence of SEQ ID NO: 2 with peptidyl carrier protein activity, and a first condensation domain comprising the amino acid sequence of SEQ ID NO: 3 or an amino acid sequence having at least 90% sequence identity with the amino acid sequence of SEQ ID NO: 3 with condensation activity in that order from the N-terminal side;
a second module having a second adenylation domain comprising the amino acid sequence of SEQ ID NO: 4 or an amino acid sequence having at least 90% sequence identity with the amino acid sequence of SEQ ID NO: 4 with adenylation activity, a first N-methyltransferase domain comprising the amino acid sequence of SEQ ID NO: 5 or an amino acid sequence having at least 90% sequence identity with the amino acid sequence of SEQ ID NO: 5 with N-methyltransferase activity, a second peptidyl carrier protein domain comprising the amino acid sequence of SEQ ID NO: 6 or an amino acid sequence having at least 90% sequence identity with the amino acid sequence of SEQ ID NO: 6 with peptidyl carrier protein activity, and a second condensation domain comprising the amino acid sequence of SEQ ID NO: 7 or an amino acid sequence having at least 90% sequence identity with the amino acid sequence of SEQ ID NO: 7 with condensation activity in that order from the N-terminal side;
a third module having a third adenylation domain comprising the amino acid sequence of SEQ ID NO: 8 or an amino acid sequence having at least 90% sequence identity with the amino acid sequence of SEQ ID NO: 8 with adenylation activity, a third peptidyl carrier protein domain comprising the amino acid sequence of SEQ ID NO: 9 or an amino acid sequence having at least 90% sequence identity with the amino acid sequence of SEQ ID NO: 9 with peptidyl carrier protein activity, and a third condensation domain comprising the amino acid sequence of SEQ ID NO: 10 or an amino acid sequence having at least 90% sequence identity with the amino acid sequence of SEQ ID NO: 10 with condensation activity in that order from the N-terminal side; and
a fourth module having a fourth adenylation domain comprising the amino acid sequence of SEQ ID NO: 11 or an amino acid sequence having at least 90% sequence identity with the amino acid sequence of SEQ ID NO: 11 with adenylation activity, a second N-methyltransferase domain comprising the amino acid sequence of SEQ ID NO: 12 or an amino acid sequence having at least 90% sequence identity with the amino acid sequence of SEQ ID NO: 12 with N-methyltransferase activity, a fourth peptidyl carrier protein domain comprising the amino acid sequence of SEQ ID NO: 13 or an amino acid sequence having at least 90% sequence identity with the amino acid sequence of SEQ ID NO: 13 with peptidyl carrier protein activity, and a fourth condensation domain comprising the amino acid sequence of SEQ ID NO: 14 or an amino acid sequence having at least 90% sequence identity with the amino acid sequence of SEQ ID NO: 14 with condensation activity in that order from the N-terminal side; and
collecting dihydrotentoxin from the culture supernatant.