Patent ID: 11952569
Assignee: SEQUENOM, INC.
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 0:
1. A kit for extracting target nucleic acid comprising:
a) a set of solid supports consisting of a first solid support capable of reversibly binding nucleic acid and a second solid support capable of reversibly binding nucleic acid;
b) a set of binding buffers consisting of a first binding buffer and a second binding buffer, wherein:
the first binding buffer is formulated to comprise guanidine (iso)thiocyanate, wherein the guanidine (iso)thiocyanate is present at a concentration of 2 M to 4 M, and
the second binding buffer is formulated to comprise guanidine (iso)thiocyanate, wherein the guanidine (iso)thiocyanate is present at a concentration above 4 M;

c) a set of wash buffers consisting of a first wash buffer, a second wash buffer, and a third wash buffer; and
d) instructions for performing the target nucleic acid extraction.