Patent ID: 11913049
Assignee: RAMON GONZALEZ
Field: Biotechnology (Chemistry)
Classification: CPC C  Y | IPC C

Claim 1:
2. The method of claim 1, wherein said enzymes catalyzing the sequence of reactions for the oxygen-independent activation and conversion to the acyl-CoA comprises:
a. the alkyl succinate synthase, wherein the alkyl succinate synthase is overexpressed;
b. an overexpressed succinyl-CoA:2-methyl-alkyl-succinyl-CoA transferase or 2-methyl-alkyl-succinyl-CoA synthetase that catalyzes the conversion of said 2-methyl-alkyl-succinate to a 2-methyl-alkyl-succinyl-CoA;
c. an overexpressed 2-methyl-alkyl-malonyl-CoA mutase that catalyzes the isomerization of said 2-methyl-alkyl-succinyl-CoA to a 2-methyl-alkyl-malonyl-CoA; and
d. an overexpressed 2-methyl-alkyl-malonyl-CoA decarboxylase that catalyzes the decarboxylation of said 2-methyl-alkyl-malonyl-CoA to the acyl-CoA.