Patent ID: 11945879
Assignee: UNIVERSITY OF LAUSANNE
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 1:
2. The multispecific antibody construct of claim 1, comprising
a) at least one mutated Fab fragment consisting of:
i) the VH and VL domains of an antibody recognizing an epitope of interest;
ii) a CH1 domain of a human immunoglobulin comprising substitution of the leucine residue at position 143 of said CH1 domain with a glutamine residue and substitution of the serine residue at position 188 of said CH1 domain with a valine residue; and
iii) a CL-kappa domain of a human immunoglobulin comprising substitution of the valine residue at position 133 of said CL-kappa domain with a threonine residue and substitution of the serine residue at position 176 of said CL-kappa domain with a valine residue; and

b) at least one mutated Fab fragment consisting of:
i) the VH and VL domains of an antibody recognizing an epitope of interest;
ii) a CH1 domain of a human immunoglobulin comprising substitution of the threonine residue at position 192 of said CH1 domain with a glutamic acid residue; and
iii) a CL-kappa domain of a human immunoglobulin comprising substitution of the asparagine residue at position 137 of said CL domain with a lysine residue and substitution of the serine residue at position 114 of said CL domain with an alanine residue, wherein the position numbers used for the CH1 and CL domains refer to Kabat numbering.