Patent ID: 11920148
Assignee: CRISPR THERAPEUTICS AG
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 0:
1. A method of producing a population of cells comprising a modification in the Angiopoietin-like 3 (ANGPTL3) gene, the method comprising:
introducing into cells (a) a guide RNA (gRNA) gRNA that targets an ANGPTL3 genomic locus or a nucleic acid encoding the gRNA, and (b) a Cas9 endonuclease or a nucleic acid encoding the Cas9 endonuclease; and
producing a population of cells that comprise a modification in the ANGPTL3 gene, wherein the gRNA comprises a spacer sequence that is an RNA sequence having the nucleic acid sequence of SEQ ID NO: 9109, 7168, 7061, 7165, or 9110 in which T is substituted for U, thereby producing the population of cells that comprise a modification in the ANGPTL3 gene.