Patent ID: 11926834
Assignee: PAIRWISE PLANTS SERVICES, INC.
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 12:
13. The method of claim 11, wherein (a) the 5′-3′ exonuclease is a fusion protein comprising the 5′-3′ exonuclease fused to a peptide tag and the Type V CRISPR-Cas effector protein is a fusion protein comprising a Type V CRISPR-Cas effector protein domain fused to an affinity polypeptide that is capable of binding to the peptide tag, (b) the 5′-3′ exonuclease is a fusion protein comprising the 5′-3′ exonuclease fused to an affinity polypeptide that is capable of binding to the peptide tag and the Type V CRISPR-Cas effector protein is a fusion protein comprising a Type V CRISPR-Cas effector protein domain fused to a peptide tag, or (c) the 5′-3′ exonuclease is a fusion protein comprising the 5′-3′ exonuclease fused to an affinity polypeptide that is capable of binding to an RNA recruiting motif and the extended guide nucleic acid is linked to an RNA recruiting motif.