Patent ID: 11959848
Assignee: VENTANA MEDICAL SYSTEMS, INC.
Field: Measurement (Instruments)
Classification: CPC G | IPC G

Claim 17:
18. A non-transitory computer-readable medium for deriving data corresponding to irregularly-shaped cells from an image of a biological sample stained with at least one stain, the non-transitory computer-readable medium storing instructions for analyzing data associated with biological objects having irregular shapes, the instructions comprising:
deriving one or more feature metrics from the image by identifying nuclei of cells depicted by the image and calculating at least one expression score based on the identified nuclei, wherein the at least one expression score is calculated based on a percentage of cells of the cells depicted by the image that are biomarker-positive cells;
segmenting the image using an image segmentation algorithm to identify a plurality of sub-regions within the image;
computing a series of representational objects that correspond to a set of sub-regions of the identified plurality of sub-regions; wherein:
each representational object of the series of representational objects identifies a particular cell type; and
each sub-region of the set of sub-regions identifies an amount of stain that exceeds a threshold amount of stain; and

associating the one or more derived feature metrics from the image with calculated coordinates of each of the series of representational objects.