Patent ID: 11913028
Assignee: WISCONSIN ALUMNI RESEARCH FOUNDATION
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 0:
1. A zinc finger-based artificial transcription factor (ATF) library comprising a plurality of elements, wherein each element comprises:
one or more zinc finger domain-encoding sequences, wherein each of the one or more zinc finger domain-encoding sequences comprises a variable domain-encoding sequence encoding a sequence selected from the group consisting of SEQ ID NO:3 (X1-S-X2-X3-L-T-X6) and positions 3-9 of SEQ ID NO:69 (X1-S-X2-X3-R-K-X6), wherein each X1, X2, X3, and X6 in each instance is independently an encoded amino acid comprising one of R, H, K, D, Q, S, T, N, E, G, P, A, I, L, M, or V, wherein the one or more zinc finger domain-encoding sequences in each element comprises at least one zinc finger domain-encoding sequence comprising a variable domain-encoding sequence encoding SEQ ID NO:3 (X1-S-X2-X3-L-T-X6);
an activation domain-encoding sequence; and
an interaction domain-encoding sequence that encodes a peptide that enables interaction with a hydrophobic face of a most N-terminal zinc finger domain encoded by the one or more zinc finger domain-encoding sequences.