Patent ID: 11952399
Assignee: AMGEN INC.
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 0:
1. A method for purifying a tumor necrosis factor receptor (TNFR):Fc fusion protein, comprising:
(a) subjecting a sample comprising the TNFR:Fc fusion protein and at least one contaminant to a chromatography medium under conditions whereby the TNFR:Fc fusion protein binds to the chromatography medium,
(b) eluting the TNFR:Fc fusion protein from the chromatography medium, and
(c) filtering the eluate from (b) through
(i) one or more adsorptive prefilters; or
(ii) one or more charged or surface modified microporous membranes; and

(d) filtering the eluate from (c) through one or more viral filtration membranes to obtain the purified TNFR:Fc fusion protein, wherein the chromatography medium (i) is a hydrophobic interaction chromatography (HIC) medium or (ii) comprises a ligand comprising a hydrophobic group and an ionic group.