Patent ID: 11913080
Assignee: THE UNIVERSITY OF NORTH CAROLINA AT CHAPEL HILL
Field: Biotechnology (Chemistry)
Classification: CPC C  Y | IPC C

Claim 14:
15. A method for detecting tumor-derived Human Papilloma Virus type 16 (HPV18) in a sample from a subject, the method comprising:
providing at least a triad of modified oligonucleotide primer/probe sets of the composition of claim 1;
fractionating a plurality of HPV DNA fragments from the sample into droplets at a concentration wherein only 0 or 1 molecule of the DNA fragments is present in each droplet;
amplifying HPV DNA in each droplet with the triad of primer/probe sets to produce amplicon signals; and
detecting in each droplet any amplicon signals;
wherein detection within a droplet of the second amplicon, but not the first or third amplicon, indicates that the HPV DNA fragment fractionated into the droplet is a tumor-derived HPV DNA fragment.