Patent ID: 11866737
Assignee: TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 6:
7. The genetically engineered bacterium for producing the L-leucine according to claim 6, characterized in that, a method for synthesizing the L-leucine with the genetically engineered bacterium through fermentation includes:
inoculating a seed culture at an inoculum size of 5-10% onto a fermentation culture medium for fermentation culture, wherein the content of dissolved oxygen is maintained at 20-40%, the pH is maintained at 6.5-7.5, the culture temperature is 30-35° C., the fermentation period is 40-48 h, and the residual sugar concentration is maintained at 0-0.4% W/V during the fermentation;
the fermentation culture medium is composed of 25 g/L glucose, 12 g/L peptone, 4 g/L yeast powder, 3.5 g/L KH2PO4, 1.5 g/L MgSO4, 15 mg/L FeSO4, 15 mg/L MnSO4 and 0.01 mg/L VB1 (Vitamin B1); the pH of the fermentation culture medium is 7.0, the pressure is 0.075 MPa, and the fermentation culture medium is subjected to high-pressure steam sterilization for 15 min.