Patent ID: 11857572
Assignee: XUANWU HOSPITAL CAPITAL MEDICAL UNIVERSITY
Field: Pharmaceuticals (Chemistry)
Classification: CPC C  A | IPC A  C

Claim 0:
1. A method for increasing a yield of a central memory T cell (Tcm), wherein an artificial antigenic epitope is added in a CAR, and a CAR-T is activated by the artificial antigenic epitope; wherein the artificial antigenic epitope is located in an extracellular domain of the CAR, between the extracellular domain and a hinge region, or between the hinge region and a transmembrane structure, and the artificial antigenic epitope does not affect a binding between the CAR and an antigen targeted by the CAR; and the method comprising:
(1) introducing the CAR into a T cell; and
(2) culturing the CAR-introduced T cell, and performing specific activation in a culture process;, wherein the specific activation comprises adding an anti-artificial antigenic epitope antibody in the culture process, and the anti-artificial antigenic epitope antibody specifically recognizes the artificial antigenic epitope; in step (2), a concentration of the anti-artificial antigenic epitope antibody is 1-50 pg/mL; a method for adding the anti-artificial antigenic epitope antibody is:
encapsulating the anti-artificial antigenic epitope antibody in a culture container; in step (2), a culture medium used for culture comprises IL-7, IL-2, and IL-15, wherein a concentration of IL-7 is 10-200 ng/mL, a concentration of IL-2 is 20-1000 IU/mL, a concentration of IL-15 is 1-50 ng/mL; wherein the method does not comprise a step of specifically sorting the CAR-introduced T cells, the artificial antigenic epitope is an E-tag comprising the amino acid sequence of KPLPEVTDEY (SEQ ID NO: 1), and the anti-artificial antigenic epitope antibody is an anti-E-tag antibody comprising the amino acid sequence of SEQ ID NO: 4.