Patent ID: 11972840
Assignee: TRIPLEBAR BIO, INC.
Field: Computer technology (Electrical engineering)
Classification: CPC G  C | IPC C  G

Claim 0:
1. A method for screening of a library of strain variants for an optimized strain variant, the method comprising:
(a) providing a plurality of partitions, wherein each partition of the plurality of partitions comprises (i) a strain variant of a library of strain variants and (ii) a first volume of less than 100 μL;
(b) subjecting each partition of the plurality of partitions to a different growth condition, wherein each growth condition of the different growth conditions comprises a combination of parameters, and each growth condition of the different growth conditions reflects an expected growth condition in a second volume, wherein the second volume is more than 100 milliliters (mL);
and
using the plurality of partitions to screen the library of strain variants for increased production of a protein using a binding assay, thereby screening for an optimized strain variant optimized for production of the protein under the different growth conditions in the second volume.