Patent ID: 11891651
Assignee: ELEMENT BIOSCIENCES, INC.
Field: Measurement (Instruments)
Classification: CPC C  G | IPC C  G

Claim 11:
12. The method of claim 1, comprising at (a):
i) providing a support having a plurality of a first surface primers immobilized thereon, wherein the first surface primers have 3′ extendible ends; and
ii) generating a plurality of immobilized single stranded nucleic acid concatemer template molecules by hybridizing a plurality of single-stranded circular nucleic acid library molecules to the plurality of immobilized first surface primers and conducting a rolling circle amplification reaction with a plurality of strand displacing polymerases, and a plurality of nucleotides which include dATP, dCTP, dGTP, dTTP and nucleotides having a scissile moiety that can be cleaved to generate an abasic site, thereby generating a plurality of immobilized single stranded nucleic acid concatemer template molecules, wherein one or more individual immobilized single stranded nucleic acid concatemer template molecules within the plurality have at least one nucleotide with a scissile moiety, and wherein individual single stranded nucleic acid concatemer template molecules are covalently joined to immobilized first surface primers.