Patent ID: 11911553
Assignee: FENWAL, INC.
Field: Medical technology (Instruments)
Classification: CPC A  B | IPC A  B

Claim 7:
8. A method of concentrating a cell suspension with a centrifuge, comprising:
mounting a syringe within a syringe holder in a cavity of a centrifuge rotor having an axis of rotation, wherein
the syringe holds a cellular suspension and comprises comprising an axial end comprising a port and a radial end closed to liquid flow, with a plunger dividing the axial and radial ends;
the syringe holder comprises an inner shell configured to fit concentrically within an outer shell having an end forming a recess when the inner shell is fully inserted into the outer shell, with the syringe holder receiving the syringe with the axial end disposed towards an axial direction relative to the axis of rotation and the radial end disposed towards a radial direction relative to the axis of rotation;

drawing a first volume of the cell suspension into the axial end of the syringe;
centrifuging the syringe containing the first volume within the syringe holder with the port of the axial end disposed closer to the axis of rotation relative to the radial end of the syringe, wherein centrifuging is performed until the first volume is separated into pelletized cells and a supernatant;
expressing off the supernatant from the syringe until the axial end of the syringe comprises the pelletized cells and a desired volume of supernatant; and
resuspending the pelletized cells in the desired volume of supernatant to arrive at a final cell product, wherein the plunger is actuated towards the axial end by air pressure being applied into the radial end and actuated towards the radial end by a vacuum being applied into the radial end.