Patent ID: 11965183
Assignee: OXFORD NANOPORE TECHNOLOGIES PLC
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 17:
18. The method of claim 1, wherein the Dda helicase comprises
(a) the part of the Dda helicase which interacts with a transmembrane pore comprises positions corresponding to 1, 2, 3, 4, 5, 6, 51, 176, 177, 178, 179, 180, 181, 185, 189, 191, 193, 194, 195, 197, 198, 199, 200, 201, 202, 203, 204, 207, 208, 209, 210, 211, 212, 213, 216, 219, 220, 221, 223, 224, 226, 227, 228, 229, 247, 254, 255, 256, 257, 258, 259, 260, 261, 298, 300, 304, 308, 318, 319, 321, 337, 347, 350, 351, 405, 415, 422, 434, 437, 438 in SEQ ID NO: 8;
(b) the part of the Dda helicase which interacts with a transmembrane pore comprises positions 1, 2, 4, 51, 177, 178, 179, 180, 185, 193, 195, 197, 198, 199, 200, 202, 203, 204, 207, 208, 209, 210, 211, 212, 216, 221, 223, 224, 226, 227, 228, 229, 254, 255, 256, 257, 258, 260, 304, 318, 321, 347, 350, 351, 405, 415, 422, 434, 437 and 438 in SEQ ID NO: 8; or
(c) the part of the Dda helicase which interacts with a transmembrane pore comprises positions 1, 2, 178, 179, 180, 185, 195, 197, 198, 199, 200, 202, 203, 207, 209, 210, 212, 216, 221, 223, 226, 227, 255, 258, 260, 304, 350 and 438 in SEQ ID NO: 8;
wherein the Dda helicase comprises a modification at one or more residues corresponding to one or more positions selected from the group consisting of (a) K194, (b) W195, (c) D198, (d) K199 and (e) E258.