Patent ID: 11913035
Assignee: PIONEER HI-BRED INTERNATIONAL, INC.
Field: Biotechnology (Chemistry)
Classification: CPC A  C | IPC C

Claim 19:
20. A method for increasing the oil content of a soybean cell comprising a polynucleotide encoding a DGAT1 polypeptide, the method comprising
a. inducing a break in the genome of the soybean cell and repairing the break, wherein the repair of the break results in at least one nucleotide substitution in the polynucleotide, the substitution encoding a non-asparagine at the position corresponding to position 473 of SEQ ID NO:2, to produce a modified polynucleotide encoding a modified polypeptide;
b. expressing the modified polypeptide in the soybean cell using an endogenous promoter of the polynucleotide, wherein the fatty acid content of the soybean cell is increased compared to a soybean cell expressing a comparable polypeptide without the substitutions.