Patent ID: 11959917
Assignee: SIEMENS HEALTHCARE DIAGNOSTICS INC.
Field: Measurement (Instruments)
Classification: CPC G  Y | IPC G

Claim 0:
1. A method of detecting an anti-Zika virus IgM antibody in a biological sample from a human subject, the method comprising a first immunoassay and a second immunoassay, wherein the first immunoassay comprises:
a) incubating a first biological sample from the human subject with a labeled Zika virus antigen and
a buffer comprising an anti-human IgG Fc antibody and a solid support comprising an anti-human IgM antibody,
a labeled Zika virus antigen,

wherein, in the presence of an anti-Zika virus IgG antibody, an anti-Zika virus IgM antibody, or an anti-Zika virus IgG antibody and an anti-Zika virus IgM antibody in the biological sample, a complex I is formed, the complex I comprising (i) the anti-Zika virus IgG antibody, the anti-Zika virus IgM antibody, or the anti-Zika virus IgG antibody and the anti-Zika virus IgM antibody, (ii) the labeled Zika virus antigen, and (iii) the solid support comprising the anti-human IgM antibody; and
b) detecting the presence of complex I, the presence of which indicates the presence of the anti-Zika virus IgM antibody, the anti-Zika virus IgG antibody, or the anti-Zika virus IgG antibody and the anti-Zika virus IgM antibody in the biological sample, and wherein the second immunoassay comprises incubating a second biological sample from the human subject with a labeled Zika virus antigen and a solid support comprising an anti-human IgM antibody, wherein anti-human IgG Fc antibody is absent from the second immunoassay, and
wherein, in the presence of an anti-Zika virus IgM antibody in the second biological sample, a complex II is formed, the complex II comprising (i) the solid support comprising the anti-human IgM antibody, (ii) the anti-Zika virus IgM antibody, and (iii) the labeled Zika virus antigen; and
d) detecting the presence or absence of complex II, the presence of which indicates the presence of the anti-Zika virus IgM antibody in the second biological sample.