Patent ID: 11898187
Assignee: NORTHWESTERN UNIVERSITY
Field: Measurement (Instruments)
Classification: CPC C  G | IPC C  G

Claim 12:
13. A method for selecting an amino acid motif that is glycosylated by an N-glycosyltransferase, the method comprising:
(a) reacting a library of peptides with an N-glycosyltransferase in the presence of a sugar donor, wherein the N-glycosyltransferase glycosylates one or more of the peptides, and wherein the peptides comprise at least 6 amino acids and have a sequence X−2-X−1-N-X+1-S/T-R-C wherein X is any amino acid;
(b) detecting glycosylation of the reacted peptides by immobilizing the reacted peptides on a substrate comprising self-assembled monolayers, and performing matrix-assisted laser desorption/ionization mass spectrometry of the immobilized reacted peptides to select the amino acid motif that is glycosylated by the N-glycosyltransferase.