Patent ID: 11946039
Assignee: METAGENOMI, INC.
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 0:
1. An engineered nuclease system comprising:
(a) an endonuclease comprising a RuvC-I domain and an HNH domain; and
(b) an engineered guide ribonucleic acid (RNA) structure capable of forming a complex with said endonuclease comprising:
(i) a guide RNA sequence capable of hybridizing to a target deoxyribonucleic acid (DNA) sequence; and
(ii) an RNA sequence capable of binding to said endonuclease, wherein said RNA sequence comprises a sequence with at least 90% sequence identity to nonvariable nucleotides of any one of SEQ ID NOs: 203, 202, 613, or 614,

wherein said endonuclease comprises a sequence with at least 70% sequence identity to SEQ ID NO: 2.