Patent ID: 11899023
Assignee: REGENERON PHARMACEUTICALS, INC.
Field: Measurement (Instruments)
Classification: CPC G | IPC G

Claim 6:
7. A method for quantifying a heterodimer species in a sample comprising a first protein and a fusion protein, said method comprising:
immobilizing an antibody specific to the first protein on a solid surface;
incubating the sample with said antibody;
capturing a precipitated sample;
collecting a flow through;
treating the precipitated sample with a first compound;
treating the flow through with a second compound;
mixing the treated precipitated sample and at least a portion of the treated flow through to form a mixture; and
analyzing the mixture using a liquid chromatography coupled to a mass spectrometer to quantify the heterodimer species in the sample,
wherein the first compound is an isotope of the second compound; and
wherein the first compound is iodoacetamide.