Patent ID: 11879843
Assignee: AKOYA BIOSCIENCES, INC.
Field: Measurement (Instruments)
Classification: CPC G | IPC G

Claim 1:
2. A system, comprising:
a wavelength-adjustable light source; and
a detector,
wherein during operation of the system:
the light source is configured to generate illumination light in one or more illumination light bands and expose a biological sample to the illumination light;
the detector is configured to measure light emission from the biological sample to obtain N>1 sample images of the biological sample in response to the illumination light, wherein each sample image corresponds to a different combination of one or more of the illumination light bands and one or more wavelength bands of the light emission, and wherein the one or more wavelength bands of the light emission define a wavelength range;
the light source is configured to generate light in a background excitation band and expose the biological sample to the light in the background excitation band; and
the detector is configured to measure light emission from the biological sample in a background spectral band to obtain a background image of the biological sample, wherein the background spectral band comprises a distribution of wavelengths having a full width at half maximum (FWHM) spectral width Δλ and a center wavelength λc within the wavelength range, and wherein at least some wavelengths within the background spectral band are within a range from λc−λ/2 to λc+λ/2; and

wherein for each of a plurality of spectral contributors in the sample exposed to the light in the background excitation band, a spectral emission intensity at each wavelength within the background spectral band is 10% or less of a maximum measured spectral emission intensity of the spectral contributor following excitation of the spectral contributor in each of the illumination light bands and the background excitation band.