Patent ID: 11926847
Assignee: SANZYME BIOLOGICS PRIVATE LIMITED
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 3:
4. A process of effecting swim-up of sperm cells using the medium claimed in claim 1, comprising:
i) providing a standard sterile test tube of 11 mm diameter used for all swim-ups;
ii) collecting a neat semen sample from a donor and liquifying for the same for 30-45 minutes;
iii) dispensing 0.7 ml of pre-heated media into the 11 mm diameter test tube;
iv) loading 0.3 ml of thoroughly mixed liquified neat sample at the bottom of the test tube;
v) inclining the test tube to about 45 degrees at 37° C. and in a highly humid environment for 30 mins;
vi) collecting 200 micro litres of swim-up fraction from the top and keeping it at room temperature in a 0.5 ml eppendorf tube for DNA fragmentation test; and
vii) storing the selectively enriched of fractions containing DNA-intact sperm cells post swim-up wherein the DFIpost swimup<DFIneat for further use.