Patent ID: 11965880
Assignee: SOMALOGIC OPERATING CO., INC.
Field: Measurement (Instruments)
Classification: CPC C  G | IPC C  G

Claim 0:
1. A method for quantifying the abundances of target proteins in a biological sample, comprising:
capturing the target proteins by exposing the biological sample to a plurality of aptamers each configured to bind to a specific protein;
isolating the aptamers that captured one of the target proteins in an aptamer-containing eluate;
forming a plurality of tri-molecular complexes by exposing the aptamers in the eluate to a plurality of capture probes each configured to hybridize to a particular aptamer, each tri-molecular complex including:
one of the aptamers from the eluate,
a first capture probe including a portion hybridized to a first portion of the aptamer, and
a second capture probe including a portion hybridized to a second portion of the aptamer, a DNA primer region, and an aptamer ID sequence corresponding to the aptamer;
separating the tri-molecular complexes from capture probes not bound to aptamers;
dissociating the capture probes in the tri-molecular complexes from the corresponding aptamers;
amplifying the aptamer ID sequences of the dissociated second capture probes;
sequencing the amplified aptamer ID sequences of the dissociated second capture probes via next-generation sequencing; and
based on data obtained by sequencing the aptamer ID sequences, determining the abundances of the target proteins in the biological sample.