Patent ID: 11885947
Assignee: WESTLAKE UNIVERSITY
Field: Measurement (Instruments)
Classification: CPC G | IPC G

Claim 10:
11. A method for tiling a lattice light sheet in a lattice light sheet microscope, wherein the lattice light sheet microscope comprises a spatial light modulator, an excitation objective, a transparent annular diaphragm and a first galvanometer, wherein an optical modulation plane of the spatial light modulator is conjugated to an image plane of the excitation objective and the spatial light modulator is configured to modulate illumination light, wherein the transparent annular diaphragm is arranged at a plane conjugated to an entrance pupil of the excitation objective to filter illumination light modulated by the spatial light modulator, and wherein the spatial light modulator is a single spatial light modulator, wherein the method comprises:
determining a corresponding optical lattice of a desired lattice light sheet by simulation calculation;
acquiring a phase map from a center section of the corresponding optical lattice and loading the phase map to the single spatial light modulator to generate an optical lattice;
acquiring a phase map from an off-center section of the corresponding optical lattice and loading the phase map to the single spatial light modulator to tile the optical lattice; and
scanning each optical lattice in an extending direction of the optical lattice to form a tiled lattice light sheet.