Patent ID: 11926854
Assignee: MERCK PATENT GMBH
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 13:
14. A method of screening for an intein complex that is suitable for use in affinity purification, the method comprising:
a) contacting a fusion protein that comprises a C-intein polypeptide joined to a target molecule by a peptide bond with the N-intein polypeptide of claim 1, under conditions in which the C-intein polypeptide in the fusion protein selectively binds to the N-intein polypeptide to form an intein complex; and
b) determining whether the target molecule is cleaved from the C-intein polypeptide under conditions which support intein activity, wherein the presence of the cleaved target molecule is indicative of a catalytically-active intein complex.