Patent ID: 11963982
Assignee: EDITAS MEDICINE, INC.
Field: Pharmaceuticals (Chemistry)
Classification: CPC A  C | IPC A  C

Claim 6:
7. A method of altering a cell, comprising: contacting the cell with
a first guide RNA (gRNA) comprising a first targeting domain sequence that differs by no more than 3 nucleotides from SEQ ID NO: 953 or SEQ ID NO:955;
a second gRNA comprising a second targeting domain sequence that differs by no more than 3 nucleotides from SEQ ID NO: 952 or SEQ ID NO:954; and
an RNA-guided nuclease;
wherein (i) the RNA-guided nuclease and the first gRNA and (ii) the RNA-guided nuclease and the second gRNA associate within a target region and generate a double stranded break (DSB) in a target nucleic acid whereby to create an indel in the target nucleic acid.