Patent ID: 11873529
Assignee: KEYGENE N.V.
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 0:
1. A kit of parts for detecting one or more single nucleotide polymorphisms (SNPs) in a plurality of target nucleotide sequences in a plurality of samples, wherein the kit of parts comprises:
i) a first probe and a second probe,
wherein the first probe comprises:
a first target specific section at its 3′-end that is complementary to a first section of the target nucleotide sequence; and
a first tag section at its 5′-end that is non-complementary to the target nucleotide sequence and comprises a first universal primer binding site,

wherein the second probe comprises:
a second target specific section at its 5′ end that is complementary to a second section of the target nucleotide sequence; and
a second tag section at its 3′-end that is non-complementary to the target nucleotide sequence and comprises a second universal primer binding site,

wherein the first and second section of the target nucleotide sequence are immediately adjacent sections; and
wherein the first probe is an allele specific probe comprising an allele specific nucleotide at its 3′-end together with an allele identifier sequence in its tag section and the second probe is a locus probe or wherein the second probe is an allele specific probe comprising an allele specific nucleotide at its 5′-end together with an allele identifier sequence in its tag section and the first probe is a locus probe;
and
ii) a first and a second primer,
wherein the first primer can hybridize to the first universal primer binding site and the second primer can hybridize to the second universal primer binding site for amplification of ligated probes, and wherein at least one of the first and second primer comprises a sample-specific identifier sequence.