Patent ID: 11946106
Assignee: GUARDANT HEALTH, INC.
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 0:
1. A method for analyzing nucleic acid molecules in a biological sample, comprising:
a) physically partitioning at least a subset of the nucleic acid molecules in the biological sample based on methylation status of the nucleic acid molecules into a plurality of partitioned sets, wherein the biological sample comprises methylated nucleic acid molecules and unmethylated nucleic acid molecules; the plurality of partitioned sets comprises a first partitioned set and a second partitioned set and the methylated nucleic acid molecules are overrepresented in the first partitioned set relative to the second partitioned set;
b) digesting the first partitioned set with at least one methylation sensitive restriction enzyme, thereby removing incorrectly partitioned molecules; and
c) sequencing at least a subset of the nucleic acid molecules of the first partitioned set.