Patent ID: 11970724
Assignee: REGENERON PHARMACEUTICALS, INC.
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 0:
1. A method for cultivating a CHO K1 cell expressing aflibercept, comprising:
(a) culturing the CHO K1 cell in a cell culture medium at a temperature of about 35° C. to 38° C. during a growth phase to form a cell culture, wherein said cell culture medium is serum-free and comprises plant hydrolysates, and is supplemented with 0.6±0.09 mM ornithine and 0.714±0.11 mM putrescine; and
(b) culturing the cell culture in the cell culture medium at a temperature of about 29° C. to 37° C. during a production phase;
wherein the cell culture has an increased viable cell culture density compared to an equivalent cell culture grown in a cell culture medium that is not supplemented with ornithine and putrescine.