Patent ID: 11965154
Assignee: INSCRIPTA, INC.
Field: Measurement (Instruments)
Classification: CPC C  B  G | IPC B  C  G

Claim 0:
1. A method for performing enrichment of cells edited by a nucleic acid-guided nuclease comprising:
(a) providing transformed cells in growth medium, wherein the cells comprise a coding sequence for a nucleic acid-guided nuclease, a sequence for a donor DNA, and a coding sequence for a gRNA, wherein at least the coding sequence for the gRNA is under the control of an inducible promoter;
(b) allowing the transformed cells to grow until the cells are in stationary phase;
(c) inducing transcription of the coding sequence for the gRNA; and
(d) allowing the cells to edit, wherein an editing efficiency is improved compared to inducing transcription of the coding sequence for the gRNA when the transformed cells are in log phase.