Patent ID: 11970731
Assignee: ROCHE SEQUENCING SOLUTIONS, INC.
Field: Organic fine chemistry (Chemistry)
Classification: CPC C | IPC C

Claim 32:
33. A method of sequencing a DNA template, the method comprising the steps of:
a. forming a DNA polymerase reaction composition comprising:
i. a DNA template,
ii. a replication primer that complexes with the template,
iii. a DNA polymerase,
iv. a mixture of nucleotides or nucleotide analogs,
v. at least one compound of formula (I),

b. incubating the DNA polymerase reaction composition under conditions allowing a DNA polymerization reaction, wherein the at least one compound of formula (I) increases the rate, fidelity or processivity of the DNA polymerase reaction; and
c. determining the sequence of the nucleotides or nucleotide analogs in the resulting polymer of nucleotides or nucleotide analogs;
wherein the compound of formula (I) is:, , wherein, independently at each occurrence:
m is 1, 2 or 3;
n is 0, 1 or 2;
p is 0, 1 or 2;
Ar1 is optionally substituted aryl;
Ar2 is selected from 5- and 6-membered monocyclic aromatic rings and 9- and 10-membered fused bicyclic rings comprising two 5- and/or 6-membered monocyclic rings fused together, where at least one of the two monocyclic rings is an aromatic ring, where
Ar2 is optionally substituted with one or more substituents selected from halide, C1-C6alkyl, C1-C6haloalkyl, E-CO2R0, E-CONH2, E-CHO, E-C(O)NH(OH), E-N(R0)2, and E-OR0, where
E is selected from a direct bond and C1-C6alkylene; and
R0 is selected from H, C1-C6alkyl and C1-C6haloalkyl,
M is selected from hydrogen, halogen and C1-C4alkyl; and
L is a linking group;
or a solvate, hydrate, tautomer, chelate or salt thereof.