Patent ID: 11913036
Assignee: CODEXIS, INC.
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 4:
5. The engineered acetate kinase of claim 1, wherein said polypeptide sequence of said engineered acetate kinase has at least 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, or 99% sequence identity to SEQ ID NO: 4, and wherein said polypeptide sequence of said engineered acetate kinase further comprises at least one substitution or substitution set at one or more positions selected from 14/38/50/75/293/296, 14/342/391, 38/46/50/75/373, 46/293, 47/342/391, 50/75/283/293/296, 50/75/296, 50/103/296/342, 50/231/373, 50/268/289/372/373, 50/283/293/296, 50/283/293/342/373, 50/293, 50/293/296, 50/372/373, 75/283/296, 75/293/296/297, 75/293/391, 75/342/371, 75/390/391, 283/296, 289, 289/372, 289/372/373, 293/342/373, 293/342/391, 293/371, 296, 296/342, 310/342/391, 342, 372, and 372/373, wherein the amino acid positions of said polypeptide sequence are numbered with reference to SEQ ID NO: 4.