Patent ID: 11945839
Assignee: HOFFMANN-LA ROCHE, INC.
Field: Pharmaceuticals (Chemistry)
Classification: CPC C  A | IPC C

Claim 1:
2. A method for separating a multispecific CrossMab antibody from a light chain (LC) mispaired variant thereof, comprising
subjecting a solution comprising said CrossMab antibody and said LC mispaired variant thereof to a hydrophobic interaction chromatography (HIC) step, thereby obtaining said CrossMab antibody depleted of said LC mispaired variant thereof,
wherein the chromatographic medium used in said HIC step comprises a matrix of particles substituted with ligands,
(i) wherein said particles have an average size of 50 μm or less in diameter and said ligands are butyl groups;
(ii) wherein said particles have an average size of from 35 μm to 60 μm in diameter and said ligands are phenyl groups; or
(iii) wherein said particles have an average size of from 35 μm to 100 μm in diameter and said ligands are polypropylene glycol (PPG) groups.