Patent ID: 11859216
Assignee: DANISCO US INC.
Field: Pharmaceuticals (Chemistry)
Classification: CPC C  A | IPC A  C

Claim 18:
19. A method of producing a tailored oligofructoside product, comprising:
providing an inulosucrase by expressing a recombinant nucleic acid encoding the inulosucrase from a host cell, wherein the recombinant nucleic acid is heterologous to the host cell, and
contacting the inulosucrase with a sucrose analogue having the glucose cap of sucrose substituted by another saccharide to produce the tailored oligofructoside product,
wherein the inulosucrase (i) comprises an amino acid sequence that is at least 90% identical to amino acids 32-453 of SEQ ID NO: 4, and (ii) catalyzes polymerization of inulin oligosaccharides containing β-(2→1) linkages,
wherein the sucrose analogue has the glucose cap of sucrose substituted by a galactose, a mannose, a fucose, or a xylose.