Patent ID: 11971403
Assignee: OIL CROPS RESEARCH INSTITUTE, CHINESE ACADEMY OF AGRICULTURAL SCIENCES
Field: Measurement (Instruments)
Classification: CPC G | IPC G

Claim 8:
9. A method of detecting 4,15-diacetoxyscirpenol, aflatoxin B1 and sterigmatocystin by the time-resolved fluorescence kit according to claim 1, comprising:
(a) adding a preprocessed to-be-tested sample solution into the sample reaction bottle containing the europium-labeled anti-4,15-diacetoxyscirpenol monoclonal antibody, the europium-labeled anti-aflatoxin B1 monoclonal antibody and the europium-labeled anti-sterigmatocystin monoclonal antibody, to provide an evenly mixed solution,
(b) inserting the immunochromatography time-resolved fluorescence test strip into the evenly mixed solution in the reaction bottle,
(c) carrying out binding reaction in the reaction bottle at 37° C. for 6 min after step (b),
(d) detecting time-resolved fluorescence intensity of the detection lines and time-resolved fluorescence intensity of the quality control line on the test strip after step (c) by using a time-resolved fluorescence immunoassay system to obtain a ratio of the time-resolved fluorescence intensity of the detection lines to the time-resolved fluorescence intensity of the quality control line on the test strip;
(e) based on a curve of relationships between the ratio of the time-resolved fluorescence intensity of the detection lines to the time-resolved fluorescence intensity of the quality control line obtained in step (d) and concentrations of 4,15-diacetoxyscirpenol, aflatoxin B1 and sterigmatocystin, obtaining quantities of 4,15-diacetoxyscirpenol, aflatoxin B1 and sterigmatocystin contained in the to-be-tested sample solution, and
finally converting these quantities into quantities of 4,15-diacetoxyscirpenol, aflatoxin B 1, and sterigmatocystin contained in a to-be-tested sample.