Patent ID: 11906523
Assignee: VENTANA MEDICAL SYSTEMS, INC.
Field: Measurement (Instruments)
Classification: CPC G  C | IPC C  G

Claim 12:
13. A stained biological sample, comprising:
(a) a binding complex comprising a target antigen, a detection probe specifically bound to the target antigen, and an enzyme covalently linked to the detection probe, wherein the enzyme comprises a peroxidase, and the detection probe comprises an antibody specific for the target antigen;
(b) a deposited complex comprising a signaling conjugate comprising a phenolic moiety covalently linked to a chromogenic moiety by a linker joining the phenolic moiety and the chromogenic moiety, wherein the phenolic moiety of the signaling conjugate is covalently bound to: (1) a location on the binding complex proximate to or directly on the enzyme; or (2) a location on the binding complex proximate to or directly on the target antigen;
wherein the stained biological sample is immobilized on a solid support, and the chromogenic moiety of the deposited complex is capable of absorbing an incident light comprising a visible light and is present in an amount sufficient to produce a signal in a visible, light spectrum which can be detected using a bright field microscopy, wherein said signal is used for a specific detection of the target antigen in the stained biological sample when said chromogenic moiety of the deposited complex is exposed to the incident light comprising the visible light, and wherein the signal is a spectral absorbance of the incident light generated by the chromogenic moiety of the deposited complex.