Patent ID: 11913905
Assignee: UNIVERSITY OF WASHINGTON
Field: Measurement (Instruments)
Classification: CPC G  B  C  Y | IPC B  C  G

Claim 17:
18. A method of detecting two or more nucleotides of a nucleic acid, comprising:
(a) providing a nanopore positioned between a cis side comprising a first conductive liquid medium and the modified nucleic acid and a trans side comprising a second conductive liquid medium;
(b) providing a nucleic acid in the first conductive liquid medium, wherein the nucleic acid comprises at least two nucleotides or set of repeat nucleotides each defined as Xn, wherein n=1-1,000,000 and wherein each X and each n may be the same or different, and wherein the nucleic acid comprises a first pendant arresting construct that comprises a duplex nucleic acid and is adjacent to an Xn;
(c) causing the first pendant arresting construct to pause the nucleic acid upon entering the nanopore from the cis side, thereby producing a first residual ion current level that represents the Xn adjacent to the first pendant arresting construct, wherein the first residual ion current level is different than a measurable ion current of the same Xn in an analyte that is not arrested;
(d) altering the first pendant arresting construct, the alteration allowing the nucleic acid to advance toward the trans side;
(e) causing a second pendant arresting construct to pause the nucleic acid upon entering the nanopore, thereby producing a second residual ion current level; and
(f) comparing the first residual ion current level and the second residual ion current level to distinguish two or more nucleotides of the nucleic acid.