Patent ID: 11926820
Assignee: GOOGLE LLC
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 0:
1. A method of identifying one or more binding events between a plurality of binders and a plurality of binding targets, comprising:
(a) incubating a plurality of known binding targets with a library of binders, where one or more binders within the library bind specifically to one or more binding targets, wherein each binder within the library comprises a target binder sequence and a unique barcode sequence indicative of the associated binder;
(b) ligating the unique barcode sequence that is specifically bound to a binding target onto a DNA barcode sequence proximal to the binding target, thereby generating a DNA barcode construct;
(c) removing the target binder sequence, thereby leaving the barcode sequence attached to the DNA barcode construct, such that the identity of the binder and, therefore, the identity of the binding target is identified;
(d) optionally, repeating steps (b)-(c);
(e) ligating sequencing adaptors onto the DNA barcode construct; and
(f) sequencing the DNA barcode construct,
thereby identifying one or more binding events between a plurality of binders and a plurality of binding targets.