Patent ID: 11906432
Assignee: ESSENLIX CORPORATION
Field: Measurement (Instruments)
Classification: CPC G | IPC G

Claim 11:
12. A device for enhancing a homogenous detection of an analyte in a cell that is in a sample, comprising:
(a) a first plate and a second plate, each has a sample contact area on its surface, wherein the sample contact surfaces contact a sample comprising a cell that contains or is suspected of containing an analyte,
(b) a detection probe that specifically binds the analyte and is capable of emitting a light at a wavelength, wherein the detection probe diffuses in the sample;
(c) an optical enhancer that binds to the cell and emits a light of a wavelength that overlaps with or within a 150 nm from the wavelength of the light emitted by the detection probe;
wherein the first and second plates sandwich the sample, the detection probe, and the optical enhancer between the two sample contact areas to form a thin layer of a thickness of 200 microns (um) or less; and
wherein the thin layer has a thickness is selected so that for a given concentration of the cell in the sample, each individual cell does not substantially overlap other cells for an imaging;
wherein the thickness of the thin layer, the concentration of the detection probe in the sample, or the concentration of the optical enhancer in the sample is selected to make, in the imaging, in the thin layer, the location having a bound detection probe is distinguishable from the locations not having the bound detection probe, wherein the bound detection probe is the detection probe bound to the analyte in the cell.