Patent ID: 11884654
Assignee: CHEMOCENTRYX, INC.
Field: Pharmaceuticals (Chemistry)
Classification: CPC C  A | IPC A  C

Claim 5:
6. A method of assaying a small organic molecule for CCR(4) antagonistic activity, said method comprising
(a) contacting the small organic molecule with cells expressing CCR(4) and a radioactive CCR(4) ligand to form a reaction mixture;
(b) aspirating the reaction mixture onto a polyethyleneimine-treated GF/B glass filter;
(c) measuring the amount radioactivity remaining on the GF/B glass filter,
wherein said method comprises performing steps (a)-(c) with a positive control sample having a formula selected from the group consisting of formula (Ie):, , or a pharmaceutically acceptable salt thereof and a pharmaceutically acceptable excipient, wherein
the bond represented by  in the ring comprising Y is a single bond or a double bond;
R1 is a member selected from the group consisting of hydrogen, C1-8 alkyl, C1-8 haloalkyl, C1-8 hydroxyalkyl, C3-8 cycloalkyl, halogen, —CN, —SO2Me and —C(O)NH2;
each R2 is a member selected from the group consisting of C1-8 alkyl, C1-8 haloalkyl, halogen, —CN and C1-8 alkoxy;
R4 is a member selected from the group consisting of hydrogen, C1-8 alkyl, C1-8 haloalkyl and C1-8 hydroxyalkyl;
the subscripts n is independently an integer from 0 to 3;
Q is a member selected from the group consisting of C and N;
Y and Z are independently selected from the group consisting of CH and N;
R6 is selected from the group consisting of H, —OH, C1-8 alkyl, C1-8 hydroxyalkyl, C1-4 alkoxy-C1-4 alkyl, —C(O)NRaRb, C1-8 alkylene-C(O)NRaRb, —NH—C1-4 alkylene-C(O)NRaRb, —C(O)—C1-4 alkylene-NRaRb, —CO2H, C1-8 alkylene-CO2H, —N(Ra)C(O)NRaRb, C1-8 alkylene N(Ra)C(O)NRaRb, —NRaRb, C1-8 alkylene-NRaRb, C1-8 alkoxy, —C(O)ORa, C1-8 alkylene-C(O)ORa, —CN, —C(O)Ra, —SO2Ra and —N(Ra)C(O)Rb;
wherein
each Ra and Rb are independently selected from the group consisting of hydrogen, C1-8 alkyl, C1-8 hydroxyalkyl, C1-8 haloalkyl, and C1-8 alkoxy; and

R7 is absent or is selected from the group consisting of H, C1-8 alkyl and C1-8 haloalkyl.