Patent ID: 11913029
Assignee: CELLINO BIOTECH, INC.
Field: Measurement (Instruments)
Classification: CPC C  B  G | IPC B  C  G

Claim 13:
14. A method for performing cell culture processes using a modular bioprocessing system, comprising:
(a) inserting, into each of one or more cell culture process modules, a respective cell culture container, wherein:
(i) the respective cell culture container is configured for performing one or more cell culture processes therein;
(ii) each of the one or more cell culture containers comprises at least one semi-transparent surface permanently attached thereto, wherein the at least one semi-transparent surface is configured to enable light-based cell imaging when illuminated with light within a first wavelength range and enable light-based cell manipulation when illuminated with light within a second wavelength range; and
(iii) each of the one or more cell culture process modules is configured to manipulate the one or more cell culture processes being performed within the respective cell culture container, wherein manipulating the one or more cell culture processes comprises performing the light-based cell manipulation, wherein the light-based cell manipulation comprises directing the light within the second wavelength toward adherent cells in the one or more cell culture containers, such that the directed light within the second wavelength is at least partially absorbed by the at least one semi-transparent surface, to selectively lyse the adherent cells in the respective cell culture container;

(b) performing, by each of one or more cell culture process modules, the one or more cell culture processes;
(c) processing, by a computing subsystem communicatively coupled to the one or more cell culture process modules, images of the one or more cell culture processes; and
(d) managing, by the computing subsystem, the one or more cell culture processes being performed within the one or more cell culture containers based at least in part on the image processing, wherein managing the one or more cell culture processes comprises controlling the one or more cell culture process modules to manipulate the one or more cell culture processes.