Patent ID: 11904317
Assignee: HIFIBIO SAS
Field: Measurement (Instruments)
Classification: CPC B  G | IPC B  G

Claim 12:
13. A method for detecting a compound of interest in a droplet, comprising:
a. providing a microfluidic system comprising:
i. at least one inlet;
ii. at least one outlet; and
iii. one or more channels;

b. injecting in said microfluidic system a stream of droplets, wherein at least one droplet comprises:
i. at least one single cell;
ii. a plurality of a first capturing agents capable of binding said at least one single cell as well as said compound of interest; and
iii. a plurality of second capturing agents, each comprising a label, wherein said plurality of second capturing agents is capable of binding said compound of interest;

c. incubating said droplets under conditions that allow the production of the compound of interest, whereby if the compound of interest is produced by said at least one single cell, it is capable of being captured by said plurality of first and second capturing agents; and
d. determining the presence of the compound of interest by detecting a presence or relocalization of said label;, wherein said plurality of the first capturing agents is bound to said at least one single cell before said at least one droplet is formed.