Patent ID: 11878060
Assignee: NOVARTIS AG
Field: Pharmaceuticals (Chemistry)
Classification: CPC A  C | IPC A  C

Claim 0:
1. A method for producing monoclonal antibodies against a target protein, comprising the steps of: (a) mixing at least one cationic lipid with a messenger RNA (mRNA) coding for the target protein or a fragment thereof, thereby forming a cationic lipid-mRNA complex; (b) administering the lipid-mRNA complex to a non-human animal; and (c) obtaining antibodies that specifically bind to the target protein from the animal, wherein said mRNA of the complex comprises pseudouridine and one or more of the following: a consensus Kozak sequence; a 7-methylguanosine cap on the 5′ end of the mRNA; a polyadenosine (polyA) tail found at the 3′ terminus of the mRNA transcript; and 5′-and 3′-untranslated regions (UTRs).