Patent ID: 11913951
Assignee: ALEXION PHARMACEUTICALS, INC.
Field: Measurement (Instruments)
Classification: CPC G | IPC G

Claim 16:
17. A method of detecting C3a in a biological sample, the method comprising:
(a) incubating the biological sample with a first and second antibody that bind to human C3a, wherein the first, but not the second, antibody binds to a neoepitope, and wherein the antibodies are FRET pairs; and
(b) performing fluorescence resonance energy transfer (FRET) to detect the FRET signal, thereby detecting C3a in the biological sample;
(c) quantifying the amount of C3a in the biological sample; and
(d) repeating steps (a), (b), and (c) sequentially over a time course,, wherein the first antibody comprises heavy chain CDR1, CDR2, and CDR3 amino acid sequences as set forth in SEQ ID NOs: 1, 2, and 3, respectively, and light chain CDR1, CDR2, and CDR3 amino acid sequences as set forth in SEQ ID NOs: 4, 5, and 6, respectively.