Patent ID: 11912734
Assignee: THE JOHNS HOPKINS UNIVERSITY
Field: Organic fine chemistry (Chemistry)
Classification: CPC C | IPC C

Claim 22:
23. The method of claim 1, wherein the method comprises:
(a) providing a nucleoside phosphoramidite comprising a 2′-deoxyribose moiety having a 3′-hydroxyl position and a 5′-hydroxyl position, wherein the nucleoside phosphoramidite has a dimethoxytrityl protecting group at the 3′-hydroxyl position and a phosphoramidite moiety at the 5′-hydroxyl position;
(b) detritylating the nucleoside phosphoramidite having a dimethoxytrityl protecting group at the 3′-hydroxyl position of (a) to form a detritylated nucleoside phosphoramidite;
(c) contacting the detritylated nucleoside phosphoramidite of (b) with an activator to form an activated detritylated nucleoside phosphoramidite;
(d) contacting the activated detritylated nucleoside phosphoramidite of (c) with one or more nucleoside residues attached to a solid phase support to form a solid support-bound product;
(e) contacting the solid support-bound product of (d) with an oxidizing agent to form an oxidized solid support-bound product;
(f) capping the oxidized solid support-bound product of (e) to form a capped solid support-bound product; and
(g) deprotecting and/or cleaving the capped solid support-bound product of (f).