Patent ID: 11963977
Assignee: VCELL THERAPEUTICS INC.
Field: Pharmaceuticals (Chemistry)
Classification: CPC A  C | IPC A  C

Claim 0:
1. A method for increasing the number of cells expressing one or more markers of pluripotency in a population comprising non-embryonic normal differentiated mammalian somatic cells not expressing markers of pluripotency comprising,
without introduction of an exogenous gene, a transcript, a protein, a nuclear component or cytoplasm, or without cell fusion,
subjecting the population of non-embryonic normal differentiated mammalian somatic cells to an effective amount of stress comprising between about 200 micromolar and about 200 millimolar adenosine triphosphate (ATP) at a pH between 4.5 and 6.8 to increase the levels of stress induced genes to increase the number of cells expressing one or more markers of pluripotency selected from the group consisting of Oct4, Nanog, E-cadherin, and SSEA in the population without lysing all of the cells, and
screening for cells expressing the one or more markers of pluripotency in the population,
wherein the cells are capable of proliferating.