Patent ID: 11921118
Assignee: THE BOARD OF TRUSTEES OF THE LELAND STANFORD JUNIOR UNIVERSITY
Field: Measurement (Instruments)
Classification: CPC C  G | IPC C  G

Claim 0:
1. A method for labeling a proteome of a cell or a portion of the proteome of the cell, the method comprising:
(a) introducing into the cell two or more variant aminoacyl-tRNA synthetases that recognize one or more noncanonical amino acids, wherein the two or more variant aminoacyl-tRNA synthetases comprise two or more of ScTyrY43G of SEQ ID NO:4, the MmPheT413G of SEQ ID NO:5, HsPheT413G of SEQ ID NO:6, and wherein the one or more noncanonical amino acids comprises 3-azido-L tyrosine (AzY), p-azido-L-phenylalanine (AzF), or a combination thereof;
(b) subsequently exposing the cell to the one or more noncanonical amino acids, thereby integrating the one or more noncanonical amino acids into the proteome or the portion of the proteome, and producing a modified proteome; and
(c) contacting the modified proteome with a detectable moiety, thereby producing a labeled proteome.