Patent ID: 11879127
Assignee: DANISCO US INC.
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 1:
2. The method of claim 1, wherein the DNA construct further comprises a downstream (3′) nucleic acid sequence encoding a polypeptide of interest (POI), wherein the polynucleotide construct comprises formula (I) or (II) in the 5′ to 3′ direction:

[5′ HR]-[lysA]-[GOI encoding POI]-[3′ HR]  (I)

or

[5′ HR]-[GOI encoding POI]-[lysA]-[3′ HR],  (II)

wherein the 5′-HR and 3′-HR are sufficiently homologous with a genomic region (locus) of the host cell to effect integration into the genome by homologous recombination.