Patent ID: 11884707
Assignee: REGENERON PHARMACEUTICALS, INC.
Field: Measurement (Instruments)
Classification: CPC C  G  A | IPC A  C  G

Claim 23:
24. A binding agent that specifically binds indoleamine 2,3-dioxygenase 1 (IDO1) produced by a method comprising
(a) contacting an epitope of IDO1 to a library of molecules wherein each member of the library comprise distinct cyclic peptides of 5-10 amino acids and an acetylene click handle, wherein the amino acids are D-amino acids, artificial amino acids, or combinations thereof, and wherein the epitope of the IDO1 comprises an azide click handle, wherein the epitope comprises the amino acid sequence GFWEDPKEFAGGSAGQSSVFQ (SEQ ID NO:1), LPPILVYADCVLANWKKKDPNK (SEQ ID NO:2), or NKPLTYENMDVLFSFR (SEQ ID NO:3);
(b) allowing a member of the library to covalently bind to the azide click handle of the epitope; and
(c) identifying the amino acid sequence of the library member that covalently binds to the azide click handle of the epitope, wherein the identified amino acid sequence is selected for forming a binding agent having affinity for the epitope of IDO1.