Patent ID: 11920194
Assignee: BIONTECH SE
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 22:
23. A method for screening for a genotype for loss of antigen presentation via major histocompatibility complex (MHC) class I, comprising: obtaining a sample containing nucleic acid from a subject, and identifying a chromosomal deletion and inversion event in a chromosome 15, wherein the deletion and inversion event results in the loss of the beta-2-microglobulin (B2M) locus, and wherein the presence of the chromosomal deletion and inversion event indicates that the subject possesses a genotype for loss of antigen presentation via MHC class L wherein the identifying comprises i) amplifying a nucleic acid sequence comprising the sequence set forth in SEO ID NO: 9 and/or a sequence complementary to SEO ID NO: 9 to generate an amplification product, (ii) contacting a probe to the amplification product, and (iii) detecting hybridization of the probe to the amplification product, wherein the amplifying step comprises amplifying a nucleic acid sequence comprising the sequence set forth in SEQ ID NO: 12 and/or a sequence complementary to SEQ ID NO: 12 to generate an amplification product.