Patent ID: 11911712
Assignee: FUJIFILM DIOSYNTH BIOTECHNOLOGIES UK LIMITED
Field: Chemical engineering (Chemistry)
Classification: CPC B  G | IPC B  G

Claim 2:
3. A method for preparing an aqueous solution of a defined pH, comprising an acid, a base and an additive, wherein the method comprises the steps of:
a) calculating a theoretical pH for the aqueous solution using the Henderson-Hasselbach equation in combination with the Debye Huckel theory;
b) calculating a ΔpH-corrected pH for the aqueous solution by comparing the theoretical pH with a pH value calculated from an equation determined from a mathematical model of the difference between the theoretical pH and a value for actual pH of each of a plurality of solutions having a different additive concentration within a range of different additive concentrations;
c) using the ΔpH-corrected pH to calculate concentrations of acid and base for the preparation of the aqueous solution including the additive using the Henderson-Hasselbach equation in combination with the Debye Huckel theory; and
d) preparing the aqueous solution including the additive using the concentrations of acid and base calculated in step c).