Patent ID: 11911765
Assignee: ILLUMINA, INC.
Field: Chemical engineering (Chemistry)
Classification: CPC B  C  G | IPC B  C  G

Claim 18:
19. The instrument of claim 18 wherein, the instrument is operable to flow the buffer reagent such that:
a total sweep volume of the buffer reagent is substantially determined by the formula ((1st FFV+2nd RFV)*N)+3rd FFV, wherein:
1st FFV is the first forward flow volume,
2nd RFV is the second reverse flow volume,
N is the total number of cycles in the plurality of cycles, and
3rd FFV is the third forward flow volume;

a total fluidic consumption of the buffer reagent is substantially determined by the formula ((1st FFV−2nd RFV)*N)+3rd FFV; and
wherein the ratio of the total sweep volume of the buffer reagent to the total fluidic consumption of the buffer reagent is equal to or greater than about 6.