Patent ID: 11921274
Assignee: CARL ZEISS MICROSCOPY GMBH
Field: Measurement (Instruments)
Classification: CPC G | IPC G

Claim 27:
28. A method for detecting emission light in a laser scanning microscope, in which emission light coming from a sample is guided by way of an imaging optical unit to a two-dimensional matrix sensor that is situated in an image plane and has a multiplicity of pixels, the method comprising:
detecting a detection point spread function, wherein the detecting includes spatially oversampling the detection point spread function using the matrix sensor,
spectrally separating the emission light coming from the sample using a dispersion device,
separately detecting the spectrally separated emission light using the matrix sensor, and
evaluating intensities measured by the pixels of a pixel region of the detection point spread function,
wherein the evaluating includes reversing the spectral separation for at least some of these pixels, and
wherein the reversing the spectral separation for the at least some pixels of the pixel region includes combining the intensity values measured by these pixels, based on a spectral intensity distribution of the emission light for a dye associated with the pixel region and a spatial intensity distribution of individual spectral components on the matrix sensor.