Patent ID: 11957102
Assignee: YEDA RESEARCH AND DEVELOPMENT CO. LTD.
Field: Biotechnology (Chemistry)
Classification: CPC A  C | IPC A  C

Claim 18:
19. A method of reducing the content of at least one steroidal alkaloid or a glycosylated derivative thereof, or of at least one steroidal saponin or a glycosylated derivative thereof, in a modified plant, the method comprising
(a) transforming at least one plant cell with at least one silencing molecule targeted to a nucleic acid sequence encoding a GAME15 cellulose synthase like protein; or
(b) mutagenizing at least one gene or a combination of genes, the genes encoding the GAME15 cellulose synthase like proteins, wherein the mutagenesis comprises introduction of one or more point mutations into the gene, or genome editing, or use of a bacterial CRISPR/CAS system, or a combination thereof,
wherein expression of the gene encoding the cellulose synthase like protein is reduced in the modified plant compared to its expression in a corresponding unmodified plant, thereby the modified plant comprises reduced content at least one steroidal alkaloid or a glycosylated derivative thereof, or of at least one steroidal saponin or a glycosylated derivative thereof, compared to the corresponding unmodified plant.