Patent ID: 11898149
Assignee: SYNGENTA PARTICIPATIONS AG
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 0:
1. A method of integrating a transgene into a genomic nuclease cleavage site in an event MIR604 transgenic maize genome, comprising introducing into an event MIR604 maize cell:
a) a first nucleic acid molecule comprising at least 100 contiguous nucleotides, wherein said at least 100 contiguous nucleotides have at least 90% identity with a target site in a nucleotide sequence comprising SEQ ID NO: 135, and further comprising a transgene; and
b) a second nucleic acid molecule comprising a nucleotide sequence encoding a nuclease for site-directed cleavage at a genomic nuclease cleavage site adjacent to a nucleotide sequence with at least 90% identity to a nucleotide sequence comprising SEQ ID NO: 135, that corresponds to the at least 100 contiguous nucleotides of (a),
under conditions wherein expression of the second nucleic acid molecule can occur to produce the nuclease and the nuclease can cleave the nucleotide sequence at the genomic nuclease cleavage site, whereby the transgene is integrated at the genomic nuclease target cleavage site in the event MIR604 maize genome.