Patent ID: 11912778
Assignee: DENALI THERAPEUTICS INC.
Field: Pharmaceuticals (Chemistry)
Classification: CPC C  A | IPC C

Claim 5:
6. The method of claim 1, wherein step (b) comprises:
(b1) generating first polynucleotide fragments comprising a first encoding region, wherein the first encoding region includes mutations to encode for an amino acid substitution, insertion, or deletion within the register at the first half of the register positions;
(b2) generating second polynucleotide fragments comprising a second encoding region, wherein the second encoding region includes mutations to encode for an amino acid substitution, insertion, or deletion within the register at the second half of the register positions; and
(b3) shuffling the first and second polynucleotide fragments during PCR amplification to mix the first and second encoding regions and form a library of sequence variants for expression.