Patent ID: 11946068
Assignee: EPIBONE, INC.
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 0:
1. A system for seeding therapeutic active ingredients (TAIs) onto a porous scaffold, comprising:
a first chamber for accommodating the porous scaffold;
a TAI storage device for storing TAIs;
a second chamber for storing TAI media;
a gas inlet for receiving gas from a compressed source;
a flow circuit coupled to (i) the first chamber, (ii) the TAI storage device, (iii) the second chamber and (iv) the gas inlet,
said flow circuit delivering the TAIs, the TAI media and the gas to the porous scaffold;
a pump for pumping at least one of the TAIs, the TAI media and the gas in the flow circuit;
a plurality of valves for regulating a delivery of the TAIs, the TAI media and the gas to the porous scaffold; and
a processor programmed to regulate seeding and culturing the porous scaffold by switching one or more valves between open and closed positions,
wherein the plurality of valves include:
a media valve configured to regulate the delivery of the TAI media from the second chamber to the porous scaffold;
a gas valve configured to regulate the delivery of the gas from the gas inlet
a TAI valve for regulating the delivery of the TAIs from the TAI storage device;
a first chamber valve for regulating the delivery of the gas to the first chamber; and
a waste valve for regulating a flow from the first chamber,

wherein the processor is programmed to perform a pre-wetting process for pre-wetting the porous scaffold with the TAI media, the pre-wetting process comprising the steps of:
(i) setting the media valve, the first chamber valve, and the waste valve to an open position while the gas valve and the TAI valve are in a closed position to pump the TAI media into the first chamber;
(ii) setting the gas valve, the first chamber valve, and the waste valve to an open position while the media valve and the TAI valve are closed to pump the gas into the first chamber; and
(iii) regulating pulsing pressurized gas bursts through the porous scaffold by repeatedly alternating each of the gas valve, the first chamber valve, and the waste valve between an open position and a closed position while the media valve and the TAI valve are in a closed position.