Patent ID: 11953421
Assignee: DIAGNOSTICA STAGO
Field: Measurement (Instruments)
Classification: CPC G | IPC G

Claim 15:
16. An analysis method for analyzing a biological sample containing biological cells including blood cells, the analysis method comprising the following steps:
passage of the biological cells of the biological sample to be analyzed into a measuring cell of a flow cytometer,
measurement of N cytometry parameters for each biological cell of the biological sample to be analyzed,
determination, for each biological cell of the biological sample to be analyzed, of a point in a N-dimensional space whose coordinates are defined depending on the cytometry parameters measured for the corresponding biological cell, where N is an integer greater than or equal to 3,
automatic clustering of the determined points into different cell clusters depending on the measured cytometry parameters, so as to define a sample cluster file,
identification of cell populations defined by the different cell clusters of the sample cluster file,
counting of the points of each cell cluster of the sample cluster file,
analysis of the sample cluster file so as to detect at least one possible anomaly in the sample cluster file,
comparison of the sample cluster file with reference cluster files, each of the reference cluster files being defined from cytometry parameters of a respective pathological or abnormal biological sample,
wherein the comparison step is carried out only when the at least one possible anomaly is detected during the analysis step.