Patent ID: 11873479
Assignee: SEATTLE CHILDREN'S RESEARCH INSTITUTE
Field: Pharmaceuticals (Chemistry)
Classification: CPC C  A | IPC A  C

Claim 0:
1. A polynucleotide encoding a fusion polypeptide, wherein said polypeptide comprises a homing endonuclease that (i) binds and cleaves a selected dsDNA target site in a mammalian cell, (ii) is selected from the group consisting of: I-LtrI, I-GpiI, I-GzeI, I-MpeMI, I-PanMI, I-CreI, I-OnuI, and I-HjeMI, and (iii) is linked by a linker domain to Trex2 or a biologically active fragment thereof, wherein said linker comprises from about 4 to 30 amino acids and is flexible such that said homing endonuclease and said Trex2 or biologically active fragment thereof retain their respective biological activities.