Patent ID: 11946085
Assignee: AMGEN INC.
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 13:
14. A method for controlling Mannose-5 glycoform content of denosumab molecules, wherein said glycoform content is measured as percentage of Mannose-5 glycoforms, and wherein the percentage of Mannose-5 glycoforms in the denosumab molecules is increased as compared to a control value, comprising:
(a) establishing a CHO cell culture in a bioreactor by inoculating at least 0.5×106 cells/mL CHO cells in a first cell culture medium on day 0, wherein said first cell culture medium is serum-free and does not comprise mannose, and wherein said CHO cells express denosumab;
(b) switching the CHO cell culture to a second culture medium at a time that is between day 3 to day 9, wherein said second culture medium comprises mannose and glucose, and wherein the ratio of mannose concentration to total hexose concentration in the second medium is between 0.1 and 0.4; and
(c) recovering the denosumab molecules from the cell culture.