Patent ID: 11932901
Assignee: BECTON, DICKINSON AND COMPANY
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 7:
8. A method of labeling nucleic acid targets in a sample, comprising:
contacting copies of a plurality of nucleic acid targets with a plurality of oligonucleotide barcodes, wherein each oligonucleotide barcode comprises a first universal sequence, a molecular label, and a target-binding region capable of hybridizing to the copies of the nucleic acid targets;
generating a plurality of barcoded nucleic acid molecules each comprising the target-binding region and a complement of the target-binding region;
hybridizing the complement of the target-binding region of each barcoded nucleic acid molecule with the target-binding region of one or more of:
(i) an oligonucleotide barcode of the plurality of oligonucleotide barcodes,
(ii) the barcoded nucleic acid molecule itself, and
(iii) a different barcoded nucleic acid molecule of the plurality of barcoded nucleic acid molecules;

extending 3′-ends of the plurality of barcoded nucleic acid molecules to generate a plurality of extended barcoded nucleic acid molecules each comprising the first molecular label and a second molecular label;
amplifying the plurality of extended barcoded nucleic acid molecules using primers capable of hybridizing to the first universal sequence and/or complements thereof, thereby generating a first plurality of barcoded amplicons, wherein the first plurality of barcoded amplicons comprises a plurality of target nucleic acid species;
providing a plurality of capture oligonucleotides capable of specifically binding to the plurality of target nucleic acid species; and
isolating capture oligonucleotides hybridized to the plurality of target nucleic acid species to generate an isolated plurality of target nucleic acid species.