Patent ID: 11898173
Assignee: SK INNOVATION CO., LTD.
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 5:
6. A recombinant strain #26-5 deposited under accession number KCTC14215BP, wherein said recombinant strain #26-5 deposited under accession number KCTC14215BP is constructed by adaptive evolution at a high lactic-acid concentration of a recombinant strain having lactic-acid-producing ability constructed by (a) deleting (i) a GPD1 gene, which is a gene encoding an enzyme that converts dihydroxyacetone phosphate to glycerol-3-phosphate, (ii) a CYB2 gene, which is a gene encoding an enzyme that converts lactate into pyruvate, (iii) an ADH gene, which is a gene encoding an alcohol dehydrogenase, and (iv) a PDC gene, which is a gene encoding a pyruvate decarboxylase, from the acid-resistant yeast YBC strain deposited under KCTC13508BP, and (b) introducing a gene encoding a lactate dehydrogenase comprising the sequence of SEQ ID NO: 1 into the acid-resistant yeast YBC strain deposited under KCTC13508BP.