Patent ID: 11912735
Assignee: CHR. HANSEN HMO GMBH
Field: Organic fine chemistry (Chemistry)
Classification: CPC C  A | IPC A  C

Claim 0:
1. A method for purifying one or more desired sialylated oligosaccharides produced by microbial fermentation and secreted into the fermentation broth, the method comprising:
i) separating cells that produced the one or more desired sialylated oligosaccharides during microbial fermentation and which are present in the fermentation broth at the end of the microbial fermentation from the fermentation broth to obtain a substantially cell-free fermentation broth by subjecting the fermentation broth to filtration or centrifugation;
ii) removing one or more cations from the substantially cell-free fermentation broth;
iii) removing one or more anionic impurities from the substantially cell-free fermentation broth;
iv) removing one or more compounds having a molecular weight lower than that of the desired sialylated oligosaccharide to be purified from the fermentation broth; and
v) spray-drying or crystallizing the desired sialylated oligosaccharide,
wherein the method further comprises a step of electrodialysis wherein:
(a) acidic conditions are applied during electrodialysis; or
(b) neutral conditions and bipolar membranes are applied during electrodialysis;

wherein the desired sialylated oligosaccharide is a sialylated human milk oligosaccharide selected from the group consisting of 3′-sialyllactose (3′-SL), 6′-sialyllactose (6′-SL), sialyllacto-N-tetraose a (LST-a), sialyllacto-N-tetraose b (LST-b), sialyllacto-N-tetraose c (LST-c), 3-fucosyl-sialyllactose (3-F-SL), disialyl-lacto-N-tetraose (DS-LNT), and fucosyl-LST-b (F-LST-b), and
wherein the purified desired sialylated oligosaccharide has a purity of ≥80% by dry-weight and possesses less than 10% water by weight.