Patent ID: 11865159
Assignee: SANOFI
Field: Pharmaceuticals (Chemistry)
Classification: CPC A  C | IPC A

Claim 21:
22. The composition of claim 2, wherein the coding sequence of the RNA encoding an IL-12sc protein consists of the sequence of SEQ ID NO: 18 with an N1-methyl-pseudouridine (m1ψ) in place of each uridine, the coding sequence of the RNA encoding an IL-15 sushi protein consists of the sequence of SEQ ID NO: 26 with an N1-methyl-pseudouridine (m1ψ) in place of each uridine, the coding sequence of the RNA encoding an IFNα protein consists of the sequence of SEQ ID NO: 23 with an N1-methyl-pseudouridine (m1ψ) in place of each uridine, and the coding sequence of the RNA encoding a GM-CSF protein consists of the sequence of SEQ ID NO: 29 with an N1-methyl-pseudouridine (m1ψ) in place of each uridine,
wherein each of the RNAs comprises:
a 5′ cap comprising m27,3′-PGppp(m12′-O)ApG or 3′-O-Me-m7G(5′)ppp(5′)G;
a 5′ UTR comprising the nucleotide sequence of SEQ ID NO: 2, 4, or 6;
a 3′ UTR comprising the nucleotide sequence of SEQ ID NO: 8; and
a poly-A tail comprising the nucleotide sequence of SEQ ID NO: 78; and

wherein the molar ratio or weight ratio of the RNAs in the composition is 1:1:1:1.