Patent ID: 11920177
Assignee: SASYA INC.
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 9:
10. The non-natural microorganism of claim 1, wherein the non-natural microorganism comprises a non-natural enzyme, wherein the non-natural enzyme comprises one or more modifications or mutations at substrate-specificity positions selected from the group consisting of leucine, isoleucine or methionine at position 361, leucine, isoleucine, methionine or tryptophan at position 336, tryptophan, tyrosine or isoleucine at position 347, alanine, leucine, isoleucine, methionine or tryptophan at position 364, tyrosine, tryptophan, leucine, isoleucine or methionine at position 368, leucine, isoleucine or methionine at position 371, leucine, isoleucine or methionine at position 362, leucine, valine or methionine at position 227, leucine, valine or methionine at position 252, phenylalanine, tryptophan or methionine at position 224, leucine, valine or methionine at position 289, tryptophan, tyrosine or isoleucine at position 323, leucine, isoleucine, tryptophan or methionine at position 367, phenylalanine, tryptophan or methionine at position 187, phenylalanine, tryptophan or methionine at position 241, isoleucine, methionine or valine at position 363, leucine at position 239, methionine, isoleucine or proline at position 251, methionine, isoleucine or proline at position 265, valine, methionine, isoleucine or leucine at position 226, phenylalanine, leucine, isoleucine or tryptophan at position 212, isoleucine, leucine or methionine at position 217, isoleucine, leucine or methionine at position 228, and leucine at position 210 of SEQ ID NO: 1 or SEQ ID NO: 6.