Patent ID: 11946067
Assignee: IFP ENERGIES NOUVELLES
Field: Biotechnology (Chemistry)
Classification: CPC C  Y | IPC C

Claim 8:
9. A process for genetically modifying by homologous recombination, a bacterium of the genus Clostridium, characterized in that the process comprises a step of transforming the bacterium by introducing into said bacterium a genetic tool, the genetic tool comprising:
a first nucleic acid encoding at least Cas9, wherein the sequence encoding Cas9 is placed under the control of an inducible promoter, and
at least a second nucleic acid allowing the expression of a repair template comprising a sequence of interest allowing, by a homologous recombination mechanism, replacement of a portion of bacterial DNA targeted by Cas9 by the sequence of interest,
in that at least one of said nucleic acids further encodes one or more guide RNAs (gRNAs) or in that the genetic tool further comprises one or more guide RNAs, each guide RNA comprising a Cas9-enzyme-binding RNA structure and a sequence complementary to the targeted portion of the bacterial DNA, and in that the first nucleic acid further comprises a sequence encoding an anti-CRISPR protein placed under the control of an inducible promoter.