Patent ID: 11927738
Assignee: PATHWARE INC.
Field: Measurement (Instruments)
Classification: CPC G | IPC G

Claim 0:
1. A method performed by a near-field Fourier ptychographic system for assessing a biological sample, comprising:
characterizing a speckled pattern to be applied by a diffuser;
positioning a biological sample relative to at least one light source such that at least one light source illuminates the biological sample;
diffusing light produced by the at least one light source, wherein the light is diffused in the speckled pattern either before the light interacts with the biological sample or after the light has interacted with the biological sample;
capturing a plurality of illuminated images with the embedded speckle pattern of the biological sample based on the diffused light;
iteratively reconstructing the plurality of speckled illuminated images of the biological sample to recover an image stack of reconstructed images;
stitching together each image in the image stack to create a whole slide image, wherein each image of the image stack at least partially overlaps with a neighboring image; and
identifying one or more features of the biological sample, wherein the one or more features are selected from a group consisting of: cell count, nucleus, edges, groupings, clump size, and a combination thereof.