Patent ID: 11905558
Assignee: LIFE TECHNOLOGIES CORPORATION
Field: Biotechnology (Chemistry)
Classification: CPC C  B | IPC C

Claim 0:
1. A sequencing system comprising an automated sequencing instrument including a preparation deck to prepare one or more extracted polynucleotide samples for loading, a loading station to load the one or more modified extracted polynucleotides on a substrate, and a sequencing station, the sequencing station to detect nucleotide incorporation using ion-sensitive field effect transistors, the automated sequencing instrument adapted to determine variant calls for the one or more extracted polynucleotide samples with a performance of at least 98.5% raw read accuracy and a run time in a range of 5 hours to 14 hours to determine variant calls when sequencing 4 extracted polynucleotide samples using a targeted assay with one DNA pool per sample and an average amplicon size in a range of 100 to 120 bases.