Patent ID: 11895961
Assignee: NUNHEMS B.V.
Field: Food chemistry (Chemistry)
Classification: CPC A  C | IPC A

Claim 13:
14. A method of screening plants or plant parts or seeds, or DNA derived therefrom, for the presence of a fragment on chromosome 7 comprising a CGMMV resistance QTL and/or on chromosome 9 comprising a CGMMV resistance QTL conferring CGMMV resistance comprises the steps of:
a) screening the genomic DNA for the SNP genotype of one or more or all of SNP13 at nucleotide 51 of SEQ ID NO: 13, InDel7.1 at nucleotide 91 of SEQ ID NO: 30 or at nucleotide 91 of SEQ ID NO: 31, corresponding to nucleotide 7,022,773 of chromosome 7 of the Watermelon Charleston Grey genome, SNP14 at nucleotide 51 of SEQ ID NO: 14, SNP15 at nucleotide 51 of SEQ ID NO: 15, SNP FM7.1 at nucleotide 71 of SEQ ID NO: 32 or 33, corresponding to nucleotide 8,733,604 of chromosome 7 of the Watermelon Charleston Grey genome, SNP16 at nucleotide 51 of SEQ ID NO: 16, SNP17 at nucleotide 51 of SEQ ID NO: 17, SNP18 at nucleotide 51 of SEQ ID NO: 18, linked to the QTL on chromosome 7, and/or for one or more of SNP19 at nucleotide 51 of SEQ ID NO: 19, SNP20 at nucleotide 51 of SEQ ID NO: 20, SNP21 at nucleotide 51 of SEQ ID NO: 21, SNP22 at nucleotide 51 of SEQ ID NO: 22, SNP FM9.1 at nucleotide 71 of SEQ ID NO: 34 or 35, corresponding to nucleotide 35,675,266 of chromosome 9 of the Watermelon Charleston Grey genome, InDel9.1 at nucleotide 91 of SEQ ID NO: 36 or at nucleotide 91 of SEQ ID NO: 37, corresponding to nucleotide 36,439,966 of chromosome 9 of the Watermelon Charleston Grey genome, SNP23 at nucleotide 51 of SEQ ID NO: 23 and SNP24 at nucleotide 51 of SEQ ID NO: 25, linked to the QTL on chromosome 9; and
b) optionally selecting a plant or plant part which comprises on chromosome 9 a Cytosine for marker SNP FM9.1 at nucleotide 71 of SEQ ID NO: 34 or 35, corresponding to nucleotide 35,675,266 of chromosome 9 of the Watermelon Charleston Grey genome and/or a Guanine for marker InDel9.1 instead of a Guanine-Cytosine at nucleotide 91 of SEQ ID NO: 36 or at nucleotide 91 of SEQ ID NO: 37, corresponding to a deletion of the Cytosine at nucleotide 36,439,966 of chromosome 9 of the Watermelon Charleston Grey genome, or selecting a plant or plant part which comprises on chromosome 7 an Adenine for marker InDel7.1 instead of an Adenine-Thymine at nucleotide 91 of SEQ ID NO: 30 or at nucleotide 91 of SEQ ID NO: 31, corresponding to nucleotide 7,022,773 of chromosome 7 of the Watermelon Charleston Grey genome and/or an Adenine at nucleotide 51 of SEQ ID NO: 14 or an Adenine at the equivalent position in a sequence comprising at least 95% sequence identity to SEQ ID NO: 14, and/or a Guanine at nucleotide 51 of SEQ ID NO: 15 or a Guanine at the equivalent position in a sequence comprising at least 95% sequence identity to SEQ ID NO: 15, and/or an Adenine for marker SNP FM7.1 at nucleotide 71 of SEQ ID NO: 32 or 33, corresponding to nucleotide 8,733,604 of chromosome 7 of the Watermelon Charleston Grey genome.