Patent ID: 11920116
Assignee: THE TRUSTEES OF THE UNIVERSITY OF PENNSYLVANIA
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 5:
6. A method of manufacturing a microdevice for culturing human cervical cells, comprising:
injecting hydrogel precursor solution comprising cervical stromal cells into the third parallel lane of the lower microchannel of the microdevice of claim 1;
injecting human cervical epithelial cells into the upper microchannel of the microdevice, resulting in an extracellular membrane coated surface of at least one epithelial monolayer;
perfusing the first parallel lane and the second parallel lane of the lower microchannel with stromal media; and
seeding vascular endothelial cells into the first parallel lane and the second parallel lane to form a lining of endothelial cells.