Patent ID: 11954856
Assignee: NANTOMICS, LLC
Field: Computer technology (Electrical engineering)
Classification: CPC G | IPC G

Claim 20:
21. A computerized method comprising:
obtaining, in at least one computer-readable memory, a whole image comprising images of cells;
segmenting the whole image into image areas based on a first global thresholding filter with respect to a first threshold;
selecting a plurality of patches associated with the image areas;
generating an intensity image based on intensity vectors for each patch in the plurality of patches by determining an optical density of each patch;
generating at least one cell mask with at least one cell seed by iteratively segmenting the intensity image based on a second global thresholding filter with respect to a second iteratively reducing threshold;
generating a distance map from the at least one cell mask via a chamfer distance transform;
distinguishing cell segments within the plurality of patches via a watershed transform of the distance map and at least one cell seed; and
configuring a device to display a cell count for at least one patch in the plurality of patches based on counting the cell segments.