Patent ID: 11867689
Assignee: ORGANOVO, INC.
Field: Measurement (Instruments)
Classification: CPC G  C | IPC C  G

Claim 12:
13. A method of testing the effect of a therapeutic agent on a renal transport molecule, comprising:
a. contacting the therapeutic agent with a three-dimensional, engineered, bioprinted, biological renal tubule model comprising:
i. a layer of renal interstitial tissue, the renal interstitial tissue comprising renal fibroblasts and endothelial cells, and
ii. a layer of renal epithelial tissue, the renal epithelial tissue comprising renal tubular epithelial cells from a subject with a disease that affects kidney function,
wherein the fibroblasts and endothelial cells are present in a ratio of fibroblasts to endothelial cells at which the renal tubule model is planar six days post-printing; and

b. measuring the transport molecule activity in the renal tubule model that has been contacted with the therapeutic agent compared to a control renal tubule model that has not been contacted with the therapeutic agent.