Patent ID: 11912765
Assignee: OBLIQUE THERAPEUTICS AB
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 0:
1. A method of generating an antibody to a protein, said method comprising:
(i) exposing the protein to limited or restricted proteolysis by contacting the protein with at least one protease to form at least one digested, deconstructed or truncated version of the protein and at least one surface-exposed peptide that is cleaved off from the protein by the action of said protease; and
(ii) identifying an antigenic epitope by identifying a surface-exposed epitope among the at least one surface-exposed peptide that is present in a region of the protein that results in a lack of, or significantly altered, biological function of the protein when the peptide is cleaved off or removed from the protein during the limited or restricted proteolysis; or
selecting at least one target region within the protein based on bioinformatics and/or known data of biological function of the protein and identifying an antigenic epitope by identifying a surface-exposed epitope among the at least one surface-exposed peptide present in said at least one target region; and

(iii) raising an antibody against the antigenic epitope.