Patent ID: 11972535
Assignee: STELLENBOSCH UNIVERSITY
Field: Measurement (Instruments)
Classification: CPC G | IPC G

Claim 0:
1. A computer-implemented method for visualising colocalised fluorescence signals in fluorescence microscopy of a sample comprising:
accessing multi-dimensional signal intensity data in the form of fluorescence data in a three-dimensional image space including signal intensity data for a first fluorescence channel and a second fluorescence channel associated with each voxel of a three-dimensional image;
calculating a regression as an errors-in-variables regression on the fluorescence data to generate a regression parameter corresponding to a degree of correlation between the signal intensity data obtained from the first and second fluorescence channels, with values of the regression parameter represented by a best fit line of points on a graph with axes representing the signal intensity values of the first and second fluorescence channels and with colourmap values varying along the regression parameter line;
mapping, for each voxel, the multi-dimensional fluorescence signal intensity data to the regression parameter line and then assigning a colourmap value to each such voxel in the three-dimensional image space based on the mapping; and
rendering the voxels in the three-dimensional image in colours related to the assigned colourmap values resulting in a visualisation of colocalisation of the fluorescence data in the three-dimensional image.