Patent ID: 11860094
Assignee: HITACHI HIGH-TECH CORPORATION
Field: Measurement (Instruments)
Classification: CPC G  C | IPC C  G

Claim 8:
9. An analysis method comprising:
separating a sample including a plurality of components by chromatography wherein the plurality of components comprise M kinds of components each of which is labeled with a different fluorescent substance, wherein the M is an integer greater than 1;
acquiring first time-series data of fluorescence signals detected in N kinds of wavelength bands for each of the different fluorescent substances, wherein N is an integer greater than 1 and M is greater than the N, in a state in which at least a portion of the plurality of components is not completely separated;
storing second time-series data of individual model fluorescence signals of each of the M kinds of components each of which is labeled with the different of fluorescent substance detected in the N kinds of wavelength bands, each second time-series data of the individual model fluorescence signals of each of the M kinds of components indicates a temporal change in fluorescence intensity in the N kinds of wavelength bands;
storing the acquired first time-series data having the detected fluorescence signals, the detected fluorescence signals including detected fluorescence signals of two or more of the M kinds of components in a space-time overlap;
performing fitting of peaks of the first time-series data with peaks of the second time-series data of each of the individual model fluorescence signals of each of the M kinds of components; and
determining which of the M kinds of components each of the plurality of components recognized in the first-time series data is based on the fitting of the first time-series data with the second time-series data of each of the individual model fluorescence signals of each of the M kinds of components.