Patent ID: 11874268
Assignee: NANOHESGARSAZAN SALAMAT ARYA NCUBATION CENTER FOR EQUIPMENT AND DEVICES
Field: Measurement (Instruments)
Classification: CPC G  B  C  H | IPC C  G  H

Claim 0:
1. A method for fabricating a biosensor for metastasis diagnosis, comprising:
coating a photoresist layer on a substrate layer;
forming a patterned region by patterning the photoresist layer utilizing a photolithography process;
forming a nano-roughened patterned region by forming a plurality of nano-features on the patterned region;
stripping the photoresist layer from the substrate layer;
forming an array of microelectrodes and an array of electrical connections on the nano-roughened patterned region by depositing a gold/titanium (Au/Ti) bilayer on the substrate layer with the nano-roughened patterned region, each electrical connection of the array of electrical connections corresponding to a respective microelectrode of the array of microelectrodes; and
forming a metastatic cell sensing trap on at least one microelectrode of the array of microelectrodes, comprising:
placing a solution of Human Umbilical Vein Endothelial Cells (HUVECs) on the biosensor; and
forcing an attachment between at least one HUVEC of the HUVECs and at least one microelectrode of the array of microelectrodes on the biosensor.