Patent ID: 11872215
Assignee: UNIVERSITY OF GEORGIA RESEARCH FOUNDATION
Field: Pharmaceuticals (Chemistry)
Classification: CPC A  C | IPC A  C

Claim 11:
12. A method of making an antibody-drug conjugate, comprising the step of conducting a cycloaddition reaction between:
a) an antibody comprising an N-linked oligosaccharide comprising at least one functionalized terminal sialoside; and
b) a functionalized cytotoxic drug molecule,, wherein one of the functionalized terminal sialoside or functionalized cytotoxic drug molecule comprises a 1,3 dipole or 1,2,4,5 tetrazine, and the other comprises a cyclooctyne or trans cyclooctene,, further comprising the step of preparing the N-linked oligosaccharide comprising at least one functionalized terminal sialoside by glycosylating an antibody comprising an N-linked oligosaccharide comprising at least one terminal galactose with a functionalized CMP-sialic acid donor in the presence of a sialyl transferase,, wherein the antibody comprising an N-linked oligosaccharide comprising at least one terminal galactose is prepared by treating an antibody with a glycosyltransferase and UDP-Gal.