Patent ID: 11878991
Assignee: TRILINK BIOTECHNOLOGIES, LLC
Field: Pharmaceuticals (Chemistry)
Classification: CPC C  A | IPC A  C

Claim 17:
18. A method for synthesizing a fully templated RNA molecule comprising the steps of:
introducing an initiating capped oligonucleotide primer, or a salt thereof, of the structure:, , wherein:
B1 is adenine or N6-methyladenine;
B10 is uracil;
R1 is H or methyl;
R2 and R3 are each OH; and
R4 is H, OH, O-methyl or a detectable marker,
into a mixture comprising a polynucleotide template and an RNA polymerase under conditions conducive to transcription by the RNA polymerase of the polynucleotide template; and
incubating said mixture for a time sufficient to allow for transcription of said template.