Patent ID: 11946077
Assignee: EMENDOBIO INC.
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 10:
11. The composition of claim 1, wherein the CRISPR nuclease comprises a sequence having at least 90% identity to the amino acid sequence of SEQ ID NO: 5, and wherein the CRISPR nuclease is a nickase formed by an amino acid substitution at D8, E502, H735 or D738 of SEQ ID NO: 5, or
wherein the CRISPR nuclease comprises a sequence having at least 90% identity to the amino acid sequence of SEQ ID NO: 5, and wherein the CRISPR nuclease is a nickase formed by an amino acid substitution at D586, H587 or N610 of SEQ ID NO: 5, or
wherein the CRISPR nuclease comprises a sequence having at least 90% identity to the amino acid sequence of SEQ ID NO: 5, and wherein the CRISPR nuclease is a catalytically inactive nuclease formed by an amino acid substitution at D8, E502, H735 or D738 of SEQ ID NO: 5, and an amino acid substitution at D586, H587 or N610 of SEQ ID NO: 5.