Patent ID: 11867689
Assignee: ORGANOVO, INC.
Field: Measurement (Instruments)
Classification: CPC G  C | IPC C  G

Claim 0:
1. A method of assessing the renal toxicity of a therapeutic agent, the method comprising:
a. contacting the therapeutic agent with a three-dimensional, engineered, bioprinted, biological renal tubule model comprising:
i. a layer of renal interstitial tissue, the renal interstitial tissue comprising renal fibroblasts and endothelial cells, and
ii. a layer of renal epithelial tissue, the renal epithelial tissue comprising renal tubular epithelial cells from a subject with a disease that affects kidney function,
wherein the fibroblasts and endothelial cells are present in a ratio of fibroblasts to endothelial cells at which the renal tubule model is planar six days post- printing;

b. measuring viability or functionality of the renal tubular epithelial cells; and
c. assessing the renal toxicity of the therapeutic agent based on the measured viability or functionality of the renal tubular epithelial cells.