Patent ID: 11866774
Assignee: BIOFIRE DIAGNOSTICS, LLC
Field: Chemical engineering (Chemistry)
Classification: CPC C  B | IPC B  C

Claim 0:
1. A method of nucleic acid extraction and multiplex PCR in a self-contained system, comprising:
(a) providing the self-contained system having, in fluid communication:
i. one or more ports, including an injector port for introducing the sample into the self-contained system,
ii. a nucleic acid preparation zone, the nucleic acid preparation zone configured for purifying nucleic acids,
iii. at least one amplification zone, the amplification zone configured for amplification of the plurality of nucleic acids that may be in the sample;

(b) introducing the sample into the self-contained system via the injector port, and closing the injector port subsequent to introducing the sample into the self-contained system;
(c) preparing the nucleic acids in the nucleic acid preparation zone, and moving the nucleic acids into the amplification zone;
(d) mixing the nucleic acids in the sample with PCR reaction components including a plurality of primer pairs configured for amplifying the targets,
(e) thermal cycling the nucleic acids in the amplification zone to create an amplification mixture, wherein mixing continues during thermal cycling, and
(f) detecting which of the plurality of nucleic acids are present in the amplification mixture.