Patent ID: 11898142
Assignee: THE BROAD INSTITUTE, INC.
Field: Measurement (Instruments)
Classification: CPC C  B  G  Y | IPC B  C  G

Claim 38:
39. A method for detecting target nucleic acids in samples, comprising:
distributing a sample or set of samples into one or more individual discrete volumes, the individual discrete volumes comprising the nucleic acid detection system of claim 1;
incubating the sample or set of samples under conditions sufficient to allow binding of the one or more guide RNAs to one or more target nucleic acids;
activating the CRISPR effector protein having collateral cleavage activity via binding of the one or more guide RNAs to the one or more target nucleic acids, wherein activating the CRISPR effector protein results in modification of the RNA-based masking construct such that a detectable positive signal is generated; and
detecting the detectable positive signal, wherein detection of the detectable positive signal indicates a presence of one or more target nucleic acids in the sample or set of samples.