Patent ID: 11913049
Assignee: RAMON GONZALEZ
Field: Biotechnology (Chemistry)
Classification: CPC C  Y | IPC C

Claim 7:
8. The method of claim 1, wherein said pathway for the regeneration of the fumarate from the acyl-CoA or the keto-acid comprises:
a. an overexpressed carboxylic acid omega hydroxylase that catalyzes the conversion of said keto-acid to an omega-hydroxy-2-keto-acid;
b. an overexpressed alcohol dehydrogenase that catalyzes the conversion of said omega-hydroxy-2-keto acid to an omega-oxo-2-keto-acid;
c. an overexpressed aldehyde dehydrogenase that catalyzes the conversion of said omega-oxo-2-keto-acid to a dicarboxylic 2-keto-acid;
d. an overexpressed ketoreductase or malate dehydrogenase that catalyzes the conversion of said dicarboxylic 2-keto-acid to malate; and
e. an overexpressed fumarase for the dehydration of said malate to fumarate.