Patent ID: 11913062
Assignee: ROCHE SEQUENCING SOLUTIONS, INC.
Field: Organic fine chemistry (Chemistry)
Classification: CPC C  G | IPC C  G

Claim 1:
2. The method of claim 1, further comprising:
(a) combining the isolated DNA composition with a uracil N-glycosylase (UNG) in a first buffer to provide a first mixture, wherein a portion of the isolated DNA composition includes at least one uracil moiety;
(b) incubating the first mixture under conditions suitable to degrade the portion of the isolated DNA composition;
(c) combining the first mixture with a dsDNA fragmentation enzyme to provide a second mixture; and
(d) incubating the second mixture under conditions suitable to fragment the isolated DNA composition,
wherein steps (b), (c), and (d) occur in the first buffer.