Patent ID: 11866772
Assignee: ROCHE MOLECULAR SYSTEMS, INC.
Field: Computer technology (Electrical engineering)
Classification: CPC C  G | IPC C  G

Claim 2:
3. The computer product of claim 2, wherein:
performing the pre-amplification procedure comprises:
inputting the volume of the plasma sample to the PCR amplification device; and
performing the estimated number of PCR cycles on the volume of the plasma sample; and

performing the dPCR experiment comprises:
inputting the portion of DNA molecules resulting from the pre-amplification procedure according to the pre-specified portion constraint to a plurality of partitions of the dPCR device;
performing PCR on the portion of DNA molecules in the plurality of partitions;
determining a first number of positive partitions for DNA fragments from the test chromosome in the plurality of partitions;
determining a second number of positive partitions for DNA fragments from one or more control chromosomes in the plurality of partitions;
determining a test statistic based on the first number of positive partitions and the second number of positive partitions; and
comparing the test statistic to a pre-specified cutoff value to determine whether the fetus has the chromosomal aneuploidy.