Patent ID: 11891629
Assignee: SNIPR TECHNOLOGIES LIMITED
Field: Pharmaceuticals (Chemistry)
Classification: CPC C  A | IPC A  C

Claim 1:
2. A method of producing a population of phages, wherein the phages are of a first type capable of infecting host cells of a first bacterial species or strain by binding a cell-surface receptor comprised by bacteria of the first species or strain, the method comprising
(a) providing a population of second bacterial cells comprising the receptor on the surface of the second cells, wherein the second cells are of a second species or strain, wherein the second species or strain is different from the first species or strain;
(b) infecting the second cells with the phages of the first type; and
(c) propagating the phages in the second cells, thereby producing the population of phages;
wherein the phages comprise a nucleotide sequence encoding crRNAs that are operable with a Cas in bacteria of the host cell strain or species to form an active CRISPR/Cas system that is capable of targeting one or more protospacer nucleotide sequences, wherein each target sequence is comprised by the genome of the host cells, whereby the crRNAs guide the Cas in host cells to modify the target sequence, thereby killing host cells or reducing host cell population growth; and
wherein when infected by the phage, the second cells do not comprise the active CRISPR/Cas system.