Patent ID: 11891671
Assignee: A9.COM, INC.
Field: Measurement (Instruments)
Classification: CPC C  B  G | IPC B  C  G

Claim 5:
6. A detection system for detecting a target virus, the detection system comprising:
a cartridge comprising an outer shell, a lysis buffer container storing a lysis buffer a swab tube, a vacuum port, a vacuum chamber fluidly connected to the vacuum port, a fluid displacement port, a hydration water chamber storing hydration water and fluidly connected to the fluid displacement port, a lysis transfer tube fluidly connected to the swab tube, a lysis well fluidly connected to the lysis transfer tube, an amplification cycle well fluidly connected to the lysis well and containing an amplification cycle bead, a detection cycle well fluidly connected to the amplification cycle well and containing a detection cycle bead, and a fluorescence reading chamber fluidly connected to the detection cycle well; wherein the swab tube is configured to receive a portion of a swab on which a biological sample is disposed; and wherein the cartridge is reconfigurable to cause a portion of the lysis buffer to contact the swab to form a sample infused lysis buffer solution;
an analysis device comprising a housing, a cartridge support assembly, a vacuum pump, a vacuum pump nozzle fluidly connected to the vacuum pump, a positive displacement pump, a positive displacement pump nozzle fluidly connected to the positive displacement pump, a light emitter, a fluorescence light detector, and a controller; wherein the cartridge support assembly comprises a cartridge support configured to receive and accommodate the cartridge, wherein the cartridge support assembly is operable to operably couple the vacuum pump nozzle to the vacuum port and the positive displacement pump nozzle to the fluid displacement port, and wherein the controller is configured to:
operate the vacuum pump to draw a portion of the sample infused lysis buffer solution through the lysis transfer tube from the swab tube to the lysis well,
operate the lysis well heating element to heat the sample infused lysis buffer solution in the lysis well to within a first temperature range for a first period of time to form a post-lysis sample solution,
operate the positive displacement pump to transfer at least a portion of the post-lysis sample solution from the lysis well and hydration water from the hydration water chamber to the amplification cycle well to rehydrate the amplification cycle bead to form an amplified sample solution in the amplification cycle well,
operate the positive displacement pump to transfer at least a portion of the amplified sample solution from the amplification cycle well to the detection cycle well to rehydrate the detection cycle bead to form a detection cycle liquid in the detection cycle well,
operate the positive displacement pump to transfer at least a portion of the detection cycle liquid from the detection cycle well to the fluorescence reading chamber,
operate the light emitter to emit a fluorescence excitation light onto the detection cycle liquid in the fluorescence reading chamber so that the fluorescence light detector generates a fluorescence light emission detection signal in response to a fluorescence light emitted by the detection cycle liquid,
generate, based on the fluorescence light emission detection signal, detection data indicative of whether the target virus is detected in the biological sample; and
transmit the detection data to an electronic device via a communication connection.