Patent ID: 11946041
Assignee: BICYCLERD LIMITED
Field: Measurement (Instruments)
Classification: CPC C  G | IPC C

Claim 0:
1. A method for screening a library of peptide ligands that bind to a cell surface, said library comprising a plurality of polypeptides covalently linked to a molecular scaffold at three or more amino acid residues, comprising the steps of:
(a) displaying said library of peptide ligands in a genetic display system, wherein the polypeptide comprises three or more reactive groups, each of which forms a covalent linkage to the molecular scaffold, and at least two loops, each of which comprises a sequence of amino acids subtended between two of said reactive groups;
(b) exposing the peptide ligands to one or more target molecules which are present in a lipid bilayer membrane system during the screening; and
(c) screening the peptide ligands for binding against the target, and selecting the ligands which bind to the target,
wherein the peptide ligands bind in a pocket on the target on the surface or close to the surface.