Patent ID: 11959086
Assignee: BASF AGRO B.V.
Field: Biotechnology (Chemistry)
Classification: CPC C  A | IPC A  C

Claim 0:
1. A recombinant chimeric nucleic acid molecule comprising a nucleic acid sequence encoding a dual transit peptide operably linked to a heterologous nucleic acid sequence encoding a heterologous polypeptide of interest,
wherein said dual transit peptide is from the genus Amaranthus and translocates the polypeptide of interest to a chloroplast or a mitochondrion,
wherein said dual transit peptide comprises an amino acid sequence having 100% sequence identity to SEQ ID NO: 1,
wherein said heterologous polypeptide of interest is a polypeptide which, when overexpressed in a plant, confers herbicide tolerance to said plant,
wherein said heterologous polypeptide of interest is from a prokaryote,
wherein said polypeptide has protoporphyrinogen oxidase (PPO) activity and confers tolerance to PPO-inhibiting herbicides, and
wherein said polypeptide having PPO activity comprises an amino acid sequence having 100% sequence identity to SEQ ID NO: 320.