Patent ID: 11969670
Assignee: ARUP LABORATORIES, INC.
Field: Measurement (Instruments)
Classification: CPC B  C  G | IPC B  C  G

Claim 0:
1. A method of quantifying an amount of free target analyte in an aqueous sample, comprising:
obtaining an aqueous sample that contains a target analyte in a free form (free target analyte) and the target analyte in a protein-bound form (protein-bound target analyte) in which the target analyte is bound to a target analyte binding protein;
passing the aqueous sample through a size exclusion chromatography matrix with a molecular weight cut off sufficient to allow the free target analyte to permeate into pores of the size exclusion chromatography matrix and exclude the protein-bound target analyte,
whereupon the free target analyte adheres to and is immobilized by the size exclusion chromatography matrix and the protein-bound target analyte does not adhere to by the size exclusion chromatography matrix;
separating the free target analyte from the protein-bound analyte by removing the protein-bound target analyte from the size exclusion chromatography matrix;
eluting the free target analyte (eluted free target analyte) from the size exclusion chromatography matrix with an organic solvent; and
quantifying the amount of the free target analyte from an amount of the eluted free target analyte in the eluent.