Patent ID: 11933790
Assignee: UNIVERSITY OF OTTAWA
Field: Measurement (Instruments)
Classification: CPC G  A  C  Y | IPC A  C  G

Claim 0:
1. A method of high throughput screening of multiple microbiota samples from a human subject, soil, or animal for metaproteomic analysis of said samples comprising:
providing proteome extracts from a plurality of microbiota samples from one or more human subjects, soil, or animals in culture;
spiking said proteome extracts with an isotope-labelled standard, which comprises a proteome of microbiota from a given human, soil, or animal microbiota sample labelled with an isotope by culturing said microbiota from a given human, soil, or animal microbiota in an isotope enriched medium;
performing a pre-screening using a metaproteomic analysis to identify changes in microbiomes of said plurality of microbiota samples;
selecting said microbiomes exhibiting changes; and
analyzing the selected microbiomes to characterize the changes.