Patent ID: 11891662
Assignee: TALIS BIOMEDICAL CORPORATION
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 6:
7. A method of detecting β-actin in a test sample, the method comprising:
(a) extracting nucleic acid from the test sample;
(b) amplifying a target sequence by reacting nucleic acid extracted in step (a) for less than ten minutes with a reaction mixture comprising a strand displacement DNA polymerase and a sequence-specific isothermal loop mediated amplification (LAMP) primer set; and
(c) detecting the presence or absence of an amplified product of step (b); wherein the presence of said amplification product is indicative of the presence of β-actin in the test sample,
wherein said sequence-specific LAMP primer set is set-29, wherein set-29 comprises primer sequences of SEQ ID NO: 132 and SEQ ID NO: 133.