Patent ID: 11920130
Assignee: THE TRUSTEES OF THE UNIVERSITY OF PENNSYLVANIA
Field: Pharmaceuticals (Chemistry)
Classification: CPC C  A | IPC C

Claim 17:
18. A method for generating a modified immune cell or precursor cell thereof, comprising:
a) introducing into the immune cell a first nucleic acid comprising a nucleic acid sequence encoding an exogenous T cell receptor (TCR) and/or a chimeric antigen receptor (CAR) comprising affinity for an antigen on a target cell; and
b) introducing into the immune cell one or more polypeptides and/or nucleic acids that downregulate a gene expression of an endogenous transcriptional modulator; wherein the endogenous transcriptional modulator is downregulated with a CRISPR system-mediated insertion and/or deletion in a gene locus encoding for the endogenous transcriptional modulator; and wherein the endogenous transcriptional modulator is:
(i) a transcription factor selected from the group consisting of SIX Homeobox 2 (SIX2) and Krüppel-like factor 4 (KLF4); or
(ii) an epigenetic regulator selected from the group consisting of histone-lysine-N-methyltransferase 2D (KMT2D) or PAXIP1 Associated Glutamate Rich Protein 1 (PAGR1).