Patent ID: 11944721
Assignee: SHANGHAI SIXTH PEOPLE'S HOSPITAL
Field: Medical technology (Instruments)
Classification: CPC A | IPC A

Claim 0:
1. A preparation method of a nerve conduit loaded with adipose-derived mesenchymal stem cells, comprising:
S1, adding polycaprolactone and polyvinylpyrrolidone into a binary organic solvent, performing ultrasonic treatment, and then adding reduced graphene oxide nanoparticles to obtain a spinning solution;
S2, electrospinning with the spinning solution obtained in the S1, and then washing for several times to obtain a semi-finished conduit product; and
S3, injecting a cell mixture comprising adipose-derived mesenchymal stem cells into the semi-finished conduit product obtained in the S2 to obtain the nerve conduit with the adipose-derived mesenchymal stem cells;
wherein the binary organic solvent is organic solvents of dichloromethane and dimethylformamide, and a volume ratio of the dichloromethane to the dimethylformamide is (2-4): 1;
wherein in the S2, the electrospinning comprises: the spinning solution is added into a syringe, a receiving bar has a receiving distance of 10 centimeters (cm)-20 cm and a rotational speed of 5 revolutions per minute (rpm)-15 rpm, a voltage of 10 kilovolts (kV)-20 kV is applied, a speed of a propelling pump is 2 milliliters per hour (mL/h)-3 mL/h, and a negative voltage applied between two ends of an insulating bar is −2 kV-−1 kV;
wherein in the S2, a detergent for the washing comprises alcohol and/or water;
wherein a surface of the nerve conduit loaded with adipose-derived stem cells has groove structures.