Patent ID: 11919929
Assignee: UNIVERSITY OF PUERTO RICO
Field: Pharmaceuticals (Chemistry)
Classification: CPC C  A | IPC A  C

Claim 9:
10. The method of claim 1, wherein the recombinant Fasciola hepatica fatty acid binding protein (rFh15) is made by:
(a) cloning cDNA of Fh15 protein in an expression vector pGEX-4T-2;
(b) propagating and expressing the cDNA in E. coli TOP10 as a fusion protein with glutathione S-transferase (GST) of Schistosoma japonicum at the amino end of the Fh15 protein; and
(c) purifying the expressed recombinant Fasciola hepatica fatty acid binding protein (rFh15).