Patent ID: 11965009
Assignee: THE JOHNS HOPKINS UNIVERSITY
Field: Pharmaceuticals (Chemistry)
Classification: CPC C  A | IPC A  C

Claim 0:
1. A method for producing aggregate-free, full-length, monomeric diphtheria toxin fusion proteins comprising:
(a) transforming bacteria with a DNA expression vector comprising:
(i) a toxP;
(ii) a mutant toxO that blocks Fe-mediated regulation of gene expression; and
(iii) a DNA sequence encoding a protein,
wherein the toxP and the mutant toxO regulate expression of the DNA sequence encoding the protein;

(b) incubating the transformed bacteria in a culture medium to allow expression of the protein that is secreted into the culture medium; and
(c) purifying the protein from the culture medium wherein the diphtheria toxin fusion protein is selected from the any one of SEQ ID NO:12, SEQ ID NO: 14 and SEQ ID NO:15.