Patent ID: 11858970
Assignee: CHINA AGRICULTURAL UNIVERSITY
Field: Pharmaceuticals (Chemistry)
Classification: CPC C  A  Y | IPC A  C

Claim 3:
4. The method according to claim 3, wherein in step 1, the pulverized material obtained by the pulverizing is sieved by a 60 to 100 mesh sieve to obtain a retentate and an undersize material, and the undersize material is collected to obtain the chili pepper seed powder;
in step 2, a degreasing solvent used in the degreasing is n-hexane;
step 3 comprises: mixing the degreased chili pepper seed meal with water, adjusting a pH value of the resulting mixture to 9 to 10 with an alkaline solution, reacting for 3 to 5 hours, then adjusting a pH value of the reaction solution to 4 to 5, reacting for 1 to 3 hours, centrifuging the reaction solution, and collecting the precipitate to obtain the crude protein extract;
the crude protein extract is subjected to an ultra-high pressure treatment in advance before the enzymolysis treatment in step 4, wherein the ultra-high pressure treatment is performed at a pressure of 100 to 400 MPa for 20 to 40 min;
the enzymolysis treatment is performed at a temperature of 30 to 50° C. under a pH value of 7 to 10 for 1 to 5 hours;
the enzyme used in the enzymolysis treatment is selected from alkaline proteases, and a mass ratio of the enzyme to the crude protein extract ranges from 1:20 to 1:50;
the enzyme inactivation is performed at 90 to 100° C. for 1 to 10 min; and
in step 5, the isolating and purifying are performed using chromatographic columns, wherein the chromatographic columns comprise an anion chromatographic column and a hydrophobic chromatographic column, wherein a mobile phase used for the anion chromatography column comprises water and NaCl, and a mobile phase used for the hydrophobic chromatographic column comprises water and a 40 to 60 v/v% methanol solution.