Patent ID: 11860100
Assignee: UNIVERSITY OF ROCHESTER
Field: Measurement (Instruments)
Classification: CPC G | IPC G

Claim 15:
16. A microscopy method utilizing an ultraviolet surface excitation microscopy system in combination with a mixed fluorescence dye solution made from a set of fluorescing stains in preselected proportions for generating the mixed fluorescence dye solution when dissolved in a preselected volume of a solvent, the method comprising:
(a) exposing a sample for microscopic analysis to the mixed fluorescence dye solution for a predetermined time period to stain features of the sample, the solution comprising at least a first fluorescing stain at a first concentration and a second fluorescing stain at a second concentration, the first fluorescing stain characterized by a first light emission spectrum when subjected to an ultraviolet light of a first wavelength of the microscopy system, the second fluorescence dye characterized by a second light emission spectrum when subjected to the ultraviolet light of the first wavelength of the microscopy system, wherein the first emission spectrum is different from the second emission spectrum;
(b) positioning the sample for imaging by the microscope, the microscope comprising an ultraviolet light source and a microscope objective;
(c) using the ultraviolet light source to simultaneously excite the first and second fluorescing stains associated with the features of the sample such that the microscope objective collects light emissions from the first and second fluorescing stains;
(d) wherein the method is configured so that the light emissions of features associated with the first fluorescing stain are imaged by the system at a first intensity and the light emissions of features associated with the second fluorescing stain are imaged by the system at a second intensity, wherein the first and second intensities are approximately the same.