Patent ID: 11905539
Assignee: CJ CHEILJEDANG CORPORATION
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 3:
4. A method of producing L-leucine, comprising:
(a) culturing a modified microorganism belonging to the genus of Corynebacterium for producing L-leucine, in a medium to produce L-leucine, wherein the microorganism comprises a polynucleotide encoding a modified polypeptide having an isopropylmalate synthase activity, wherein glycine at position 561 from a N-terminus of a polypeptide consisting of the amino acid sequence of SEQ ID NO: 1 is substituted with aspartic acid in the modified polypeptide, wherein the modified microorganism has a resistance to norleucine or L-leucine, and wherein a native promoter of a polynucleotide encoding the modified polypeptide in the microorganism is substituted with a promoter which enhances an expression of the polynucleotide compared to the native promoter; and
(b) recovering L-leucine from the cultured microorganism or the cultured medium.