Patent ID: 11891651
Assignee: ELEMENT BIOSCIENCES, INC.
Field: Measurement (Instruments)
Classification: CPC C  G | IPC C  G

Claim 39:
40. The method of claim 1, wherein the primer extension reaction at step (c) comprises
contacting the plurality of retained immobilized concatemer molecules with a plurality of soluble amplification primers, a plurality of nucleotides and a plurality of primer extension polymerases, under conditions suitable to hybridize the plurality of soluble amplification primers to the plurality of retained immobilized concatemer template molecules and a conducting polymerase-catalyzed primer extension reaction, thereby generating a plurality of forward extension strands, wherein the soluble amplification primers hybridize with the soluble amplification primer binding sequence in the retained immobilized concatemer molecules,
wherein the plurality of nucleotides comprise dATP, dGTP, dCTP and dTTP but lacks dUTP,
wherein in the plurality of primer extension polymerases is tolerant of uridine-containing template strands, and
wherein the soluble sequencing primers hybridize with the forward sequencing primer binding sequence in the retained immobilized concatemer molecules.