Patent ID: 11865203
Assignee: SAMI LABS LIMITED
Field: Pharmaceuticals (Chemistry)
Classification: CPC A | IPC A

Claim 0:
1. A method of therapeutic management of UV induced damage in a subject's skin, said method comprising contacting skin fibroblast cells in the subject's skin with an effective concentration of a composition containing a partially purified extracellular metabolite preparation from Bacillus coagulans MTCC 5856, to bring about the effect of protection against UV-A and UV-B induced cell damage and apoptosis, wherein the partially purified extracellular metabolite preparation is obtained using a process comprising steps of:
a) inoculating a culture of Bacillus coagulans MTCC 5856 into 1.0 liter of Glucose Yeast Extract Acetate broth medium or MRS broth containing 0.5% Tween 80 or Corn steep powder media to initiate bacterial fermentation;
b) allowing the bacterial fermentation in the inoculated medium of step a) to proceed for 24-48 h at 37° C. with 120 rpm;
c) centrifuging the fermentation broth of step b) at 4000-7000 rpm and collecting supernatant;
d) concentrating the supernatant of step c) 10-fold by using a rotary evaporator at 50° C.;
e) adding 150 ml of chilled acetone drop by drop to 100 ml of the 10-fold concentrated supernatant of step d), followed by mixing to form a supernatant-acetone mixture;
f) incubating the supernatant-acetone mixture of step e) at 0° C. for 30 minutes followed by centrifuging at 7000-8000 rpm to collect a pellet;
g) discarding the pellet obtained in step f) and collecting 60% (v/v) acetone-saturated supernatant;
h) concentrating the acetone-saturated supernatant in step g) 4-fold by rotary evaporator;
i) adjusting the pH of the supernatant of step h) to 5.0 by using 4N HCl; and
j) freeze drying the supernatant of step i) to obtain the partially purified extracellular metabolite preparation.