Patent ID: 11912773
Assignee: GENENTECH, INC.
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 18:
19. A method of producing an isolated heteromultimeric protein or isolated IgG antibody, the method comprising culturing a prokaryotic host cell comprising an expression vector encoding the heteromultimeric protein or the IgG antibody, and recovering the heteromultimeric protein or the IgG antibody from the cell culture, wherein the heteromultimeric protein or the IgG antibody each comprises an Fc region comprising substitution mutations at residues 241 and 243 on at least one heavy chain, wherein said substitution mutations are selected from the group consisting of (a) F241R and F243 S and (b) F241S and F243R, wherein numbering is according to the EU numbering scheme, and wherein no amino acid residue of any Fc polypeptide of the heteromultimeric protein or the IgG antibody is glycosylated.