Patent ID: 11877556
Assignee: NORTH CAROLINA STATE UNIVERSITY
Field: Biotechnology (Chemistry)
Classification: CPC A  C | IPC A  C

Claim 10:
11. A Nicotiana tabacum plant comprising
(a) a first mutation positioned between nucleotide 115 and nucleotide 1053 as compared to SEQ ID NO: 4 at a first locus encoding a CYP82E10 nicotine demethylase having at least 95% sequence identity to the full length of SEQ ID NO: 4;
(b) a second mutation at a second locus encoding a CYP82E4 nicotine demethylase, wherein the second mutation results in reduced expression or function of the CYP82E4 nicotine demethylase as compared to a control Nicotiana tabacum plant lacking the second mutation; and
(c) a third mutation at a third locus encoding a CYP82E5v2 nicotine demethylase, wherein the third mutation results in reduced expression or function of the CYP82E5v2 nicotine demethylase as compared to a control Nicotiana tabacum plant lacking the third mutation.