Patent ID: 11913071
Assignee: SINGULAR GENOMICS SYSTEMS, INC.
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 0:
1. A method of amplifying a double-stranded polynucleotide, said method comprising:
(a) contacting the double-stranded polynucleotide comprising a first strand hybridized to a second strand with an invasion primer, wherein the first strand and the second strand are attached to a solid support;
(b) hybridizing the invasion primer to the second strand, wherein the invasion primer is not covalently attached to the solid support;
(c) extending the invasion primer hybridized to the second strand with a polymerase to generate an invasion strand, thereby displacing the first strand;
(d) hybridizing the first strand to a first immobilized primer on the solid support, wherein the first immobilized primer is complementary to a sequence of the first strand; and
(e) extending the first immobilized primer with a polymerase to generate a first amplification product.