Patent ID: 11891430
Assignee: HOFFMANN-LA ROCHE, INC.
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 1:
2. A method for producing an immunoglobulin comprising the following steps
a) cultivating a cell comprising a nucleic acid encoding an immunoglobulin,
b) recovering the immunoglobulin from the cell or the cultivation medium,
c) purifying the immunoglobulin with one or more chromatography steps, and providing an immunoglobulin solution,
d) optionally adding a sugar, an amino acid and/or a detergent to the solution,
e) concentrating the immunoglobulin solution to a concentration of 100 g/l or more with a method selected from diafiltration or tangential-flow filtration, and
f) applying the immunoglobulin solution of the previous step to a combination of a first and second filter unit, whereby the first filter unit comprises a pre-filter with a pore size of 3.0 μm and a main-filter with a pore size of 0.8 μm and the second filter unit comprises a pre-filter with a pore size of 0.45 μm and a main-filter with a pore size of 0.22 μm with a pressure of from 0.1 to 4.0 bar, and thereby producing an immunoglobulin.