Patent ID: 11972620
Assignee: nan
Field: Measurement (Instruments)
Classification: CPC G | IPC G

Claim 16:
17. A method comprising:
(A) receiving, with a computer system, a plurality of images of a moving cell in a sample obtained by an imaging flow cytometer, the plurality of images being acquired across multiple imaging modes including a side scatter image and a fluorescence image, each of the plurality of images being spatially well aligned with each other;
(B) selecting, with the computer system, a first image from the plurality of images of the moving cell;
(C) segmenting, with the computer system, the first image into subcomponents the first image into subcomponents representing parts of the moving cell;
(D) segmenting, with the computer system, a second image from the plurality of images of the moving cell into at least one subcomponent representing a part of the moving cell to generate a subcomponent mask, wherein the second image is of a different imaging mode than the first image;
(E) spatially correlating, with the computer system, the subcomponent mask to the segmented first image;
(F) applying, with the computer system, a graph cut segmentation to the second image using the subcomponent mask as a foreground object marker for the segmented first image to generate image data having increased location accuracy of the subcomponents of the moving cell; and
(G) reprocessing, with the computer system, the first image using the image data generated in (F) to identify cell morphology of the moving cell in the first image.