Patent ID: 11898141
Assignee: THE BROAD INSTITUTE, INC.
Field: Biotechnology (Chemistry)
Classification: CPC C  G  B | IPC C  G

Claim 9:
10. A method of optimizing a combination of nucleic acid elements for production of an optimum desired phenotype, the method comprising:
retrieving a plurality of sequences of:
the set of sequence-verified nucleic acid elements of claim 1; and/or
(ii) a pool of higher-order nucleic acid constructs comprising the sequence-verified nucleic acid elements of claim 1;
assembling the plurality of retrieved sequences to construct a biological pathway assembly;
testing each of the assembled biological pathway assemblies fora desired phenotype;
determining which characteristic or characteristics of the assembled biological pathway contributes to the desired phenotype by comparing multiple assemblies; and
repeating the retrieving, assembling, testing, and determining until an assembled biological pathway is identified that produces said optimum desired phenotype.