Patent ID: 11970729
Assignee: QUANTUM-SI INCORPORATED
Field: Measurement (Instruments)
Classification: CPC C  G | IPC C  G

Claim 0:
1. A method of determining the sequence of a template nucleic acid comprising:
(i) exposing a complex in a target volume, the complex comprising the template nucleic acid, a primer, and a polymerizing enzyme, to a plurality of types of luminescently labeled nucleotides, wherein each type of luminescently labeled nucleotide has a different luminescent lifetime and/or luminescent intensity;
(ii) directing a series of pulses of one or more excitation energies towards a vicinity of the target volume;
(iii) detecting a plurality of emitted photons from luminescently labeled nucleotides during sequential incorporation into a nucleic acid comprising the primer; and
(iv) identifying the sequence of incorporated nucleotides by determining the lifetime and intensity of the luminescently labeled nucleotides.