Patent ID: 11905541
Assignee: DNA SCRIPT SAS
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 7:
8. A kit for enzymatically synthesizing a polynucleotide comprising an initiator attached to a support by a 5′ end and having a 3′-penultimate deoxyinosine; a 3′-terminal nucleotide having a free 3′-hydroxyl; an endonuclease V capable of cleaving an initiator-polynucleotide conjugate 3′ of a terminal nucleotide of the initiator; the template-free polymerase terminal deoxynucleotidyl transferase; and a 3′-O-blocked nucleoside triphosphate for one or more of deoxyadenosine, deoxyguanosine, thymidine, deoxyuridine and deoxycytidine.