Patent ID: 11946077
Assignee: EMENDOBIO INC.
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 5:
6. The composition of claim 2, wherein the composition is an engineered, non-naturally occurring composition comprising a CRISPR associated system comprising:
one or more RNA molecules, or one or more nucleotide sequences encoding the one or more RNA molecules, wherein at least one of the one or more RNA molecules comprises a guide sequence portion linked to a direct repeat sequence, wherein the guide sequence portion is capable of hybridizing with a target sequence; and
a CRISPR nuclease comprising an amino acid sequence having at least 90% identity to the amino acid sequence of SEQ ID NO: 5, or a nucleic acid molecule comprising a sequence encoding the CRISPR nuclease; and, wherein the one or more RNA molecules hybridize to the target sequence, wherein the target sequence is adjacent to the 3′ end of a complimentary sequence of a NGR or NGG Protospacer Adjacent Motif (PAM), and the one or more RNA molecules form a complex with the CRISPR nuclease.