Patent ID: 11905551
Assignee: BIO-RAD LABORATORIES, INC.
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 0:
1. A method of performing multiple displacement amplification, the method comprising,
providing a plurality of oligonucleotides, each oligonucleotide comprising a 3′ random sequence of at least four contiguous nucleotides, a barcode sequence, and one or more intervening sequences, the oligonucleotides annealed to a complementary nucleic acid that is complementary to some or all of the intervening sequence, wherein the complementary nucleic acid is not complementary to the barcode sequence or the 3′ random sequence, leaving the 3′ random sequence to be single-stranded, wherein the intervening sequence is:
(i) 5′ to the barcode sequence, or
(ii) a sequence that divides the barcode into two or more barcode portions, wherein the barcode portions are separated by an intervening sequence; and
wherein at least part of the method is performed in partitions and the barcode is a partition-specific barcode, or the oligonucleotides further comprise a 5′ tag sequence that is covalently linked to a solid support bead and the barcode is a bead-specific barcode;
contacting the plurality of oligonucleotides to sample DNA under conditions in which the complementary nucleic acid anneals to an oligonucleotide of said plurality of oligonucleotides and allows for annealing of the 3′ random sequence to the DNA; and
extending the 3′ random sequence in a template-dependent manner with a strand-displacing polymerase to generate extended oligonucleotides comprising a 3′ sequence complementary to the DNA.