Patent ID: 11944936
Assignee: WATERSEP BIOSEPARATIONS LLC
Field: Chemical engineering (Chemistry)
Classification: CPC B | IPC B

Claim 0:
1. A method of single pass, cross-flow diafiltration, comprising:
a. providing three or more filtration segments fluidly connected in series, each having an upstream side and a downstream side; wherein each of the three or more filtration segments comprises hollow fiber filter membranes
b. providing a first pump, mounted to urge fluid flow to the first filtration segment of the three or more filtration segments;
c. providing one or more points of introduction of a diadiluent, each at a point between two or more upstream segments and one or more downstream segments of the three or more filtration segments, wherein the two or more upstream segments have produced a volume of permeate;
d. providing one or more further pumps, each mounted between the three or more filtration segments to provide a single pump that urges fluid flow of retentate and introduced diadiluent from the one or more points of introduction of the diadiluent;
e. measuring the volume of permeate from at least two of the two or more upstream segments; and
f. introducing a volume of diadiluent to a retentate from the two or more upstream segments,
wherein the volume of diadiluent that is introduced to the retentate is substantially equal to a sum of the measured volume of permeate from the at least two upstream segments.