Patent ID: 11926833
Assignee: LIVING CARBON PBC
Field: Biotechnology (Chemistry)
Classification: CPC C  Y | IPC C

Claim 0:
1. A transgenic tree plant cell comprising:
endogenous glycolate transporter Plgg1 and
one or more heterologous polynucleotides comprising:
a first promoter operably linked to a polynucleotide sequence encoding a glycolate dehydrogenase;
a second promoter operably linked to a polynucleotide sequence encoding a malate synthase; and
an RNAi polynucleotide targeting the endogenous glycolate transporter Plgg1, wherein the RNAi polynucleotide comprises a sense strand comprising at least 21 contiguous nucleotides of the endogenous glycolate transporter Plgg1 and an antisense strand that is fully complementary to the at least 21 contiguous nucleotides of the sense strand, wherein the sense strand and the antisense strand form a dsRNA, wherein expression of the endogenous glycolate transporter Plgg1 in the transgenic tree plant cell is about 20% to 80% of expression of endogenous glycolate transporter Plgg1 in a tree plant cell that is not transformed with the RNAi polynucleotide targeting the endogenous glycolate transporter Plgg1, and wherein the transgenic tree plant cell is from a tree selected from Populus, Pinus taeda, and Picea abies.