Patent ID: 11946911
Assignee: SHIMADZU CORPORATION
Field: Measurement (Instruments)
Classification: CPC G | IPC G

Claim 9:
10. A method for rapidly detecting vitamin D in an oil or fat or a biological sample, comprising:
step S1 of preparing a sample to be analyzed;
step S2 of separating vitamin D in the sample to be analyzed by using a multidimensional chromatography system; and
step S3 of detecting the vitamin D separated in the step S2, wherein:
in the step S2, the multidimensional chromatography system includes a supercritical chromatography part and a reverse phase liquid chromatography part which are sequentially connected;
the reverse phase liquid chromatography part includes two reverse phase liquid chromatography columns;
the two reverse phase liquid chromatography columns include a first reverse phase liquid chromatography column and a second reverse phase liquid chromatography column;
the supercritical chromatography part includes a supercritical mobile phase and a modifier;
the modifier is selected from alcohol, nitrile, and an aqueous solution of alcohol, or nitrile; and
in the step S2, a weakly polar substance adsorbed to a supercritical chromatography column is transported to the first reverse phase liquid chromatography column by only an action of the modifier to remove a supercritical mobile phase flowing out of the supercritical chromatography part in the first reverse phase liquid chromatography column, and the weakly polar substance is transported to the second reverse phase liquid chromatography column to separate a component.