Patent ID: 11946040
Assignee: THE GENERAL HOSPITAL CORPORATION
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 0:
1. An adenine base editor (ABE) variant capable of deaminating adenine in DNA comprising an adenosine deaminase and a programmable DNA binding domain, wherein the adenosine deaminase comprises one or more Escherichia coli TadA monomers, wherein each E. coli TadA monomer has at least 90% sequence identity to the amino acid sequence of SEQ ID NO: 1 or the amino acid sequence of SEQ ID NO: 34, and wherein at least one of the one or more E. coli TadA monomers comprise one or more mutations at amino acid residue positions that correspond to amino acid residues 10, 11, 13, 21, 25, 26, 49, 58, 71, 72, 74, 77, 82, 109, 111, 122, 128, 129, 139, 140, 143, 148, 150, or 153 of the amino acid sequence of SEQ ID NO: 1 or the amino acid sequence of SEQ ID NO: 34.