Patent ID: 11913885
Assignee: JFE ADVANTECH CO., LTD.
Field: Measurement (Instruments)
Classification: CPC G  A  C  Y | IPC A  G

Claim 0:
1. A method for calculating an abundance of specific species of phytoplankton, the method comprising:
irradiating a reference sample containing a phytoplankton group with excitation light, the phytoplankton group containing a plurality of kinds of phytoplankton, the plurality of kinds of phytoplankton possibly including specific species of phytoplankton, and the specific species of the phytoplankton emitting fluorescence by absorbing the excitation light;
measuring an intensity of the fluorescence emitted from the reference sample in each of two wavelength bands and calculating a reference sample intensity ratio that is a ratio of intensities of these two;
measuring a reference sample total fluorescence intensity that is an intensity in a substantially entire wavelength band of the fluorescence emitted from the reference sample;
counting a reference abundance of the specific species of the phytoplankton contained in the reference sample;
calculating an other species plankton intensity ratio, that is a ratio of intensities in the two wavelength bands of the fluorescence emitted from other species of a phytoplankton other than the specific species of the phytoplankton in the phytoplankton group, based on the reference sample intensity ratio, the reference sample total fluorescence intensity, and the reference abundance;
irradiating an analysis sample, which is expected to have similarity with the reference sample with respect to composition of the phytoplankton group, with excitation light;
measuring an intensity of the fluorescence emitted from the analysis sample in each of the two wavelength bands and calculating an intensity ratio that is a ratio of intensities of these two;
measuring a total fluorescence intensity that is an intensity in a substantially entire wavelength band of the fluorescence emitted from the analysis sample; and
calculating an abundance of the specific species of the phytoplankton that may be contained in the analysis sample based on the other species plankton intensity ratio, the intensity ratio, and the total fluorescence intensity.