Patent ID: 11911415
Assignee: EDITAS MEDICINE, INC.
Field: Pharmaceuticals (Chemistry)
Classification: CPC A  C  G | IPC A  C  G

Claim 12:
13. The method of claim 1, wherein the Cas9 molecule is selected from the group consisting of:
(i) a Cas9 molecule comprising N-terminal RuvC-like domain cleavage activity, and lacking HNH-like domain cleavage activity;
(ii) a Cas9 molecule comprising an amino acid mutation at an amino acid position corresponding to amino acid position N863 of Streptococcus pyogenes Cas9;
(iii) a Cas9 molecule comprising HNH-like domain cleavage activity, and lacking N-terminal RuvC-like domain cleavage activity; and
(iv) a Cas9 molecule comprising an amino acid mutation at an amino acid position corresponding to amino acid position D10 of Streptococcus pyogenes Cas9.