Patent ID: 11899021
Assignee: PROMEGA CORPORATION
Field: Measurement (Instruments)
Classification: CPC G  C | IPC C  G

Claim 0:
1. A method to detect an interaction between a ligand and a target protein, comprising the steps of:
(a) incubating (i) a fusion of a target protein and a peptide tag comprising 70% or greater sequence identity with VSGWRLFKKIS (SEQ ID NO: 5) with (ii) a complementary polypeptide comprising 70% or greater sequence identity with SEQ ID NO: 6, wherein the peptide tag and the complementary polypeptide are capable of forming a bioluminescent complex:
(i) in the presence of the ligand to produce a test sample, and
(ii) in the absence of the ligand, to produce a control sample;

(b) treating said test and control samples under conditions that cause the target protein to unfold partially or completely unfold;
(c) measuring signal from the bioluminescent complex in said test and control samples; and
(d) comparing the measurement made in step (c) between the test and control samples, wherein alteration of the signal from said bioluminescent complex in the test sample compared to the control sample indicates the presence of the interaction between the ligand and the target protein.