Patent ID: 11925695
Assignee: LANTHEUS MEDICAL IMAGING, INC.
Field: Pharmaceuticals (Chemistry)
Classification: CPC A  C | IPC A  C

Claim 11:
12. A method of ultrasound contrast imaging a subject using an ultrasound contrast agent having reduced batch-to-batch loss of total and individual phospholipid content comprising
a) controlling the calcium and/or magnesium concentration across multiple batches of a phospholipid solution to less than 0.7 ppm, wherein the phospholipid solution comprises one or more phospholipids in propylene glycol, and combining one of the multiple batches of the phospholipid solution with an aqueous solution to form a phospholipid suspension,
b) activating the phospholipid suspension with a perfluorocarbon gas to form lipid-encapsulated gas microspheres,
c) administering the lipid-encapsulated microspheres to a subject, and
d) obtaining an ultrasound image of the subject,
wherein controlling the calcium and/or magnesium concentration across multiple batches comprises
(i) analyzing the calcium and/or magnesium concentration of each batch,
(ii) forming the phospholipid solution with the one or more phospholipids having known calcium and/or magnesium concentration, or
(iii) measuring calcium and/or magnesium concentration of the one or more phospholipids used to form the phospholipid solution.