Patent ID: 11971403
Assignee: OIL CROPS RESEARCH INSTITUTE, CHINESE ACADEMY OF AGRICULTURAL SCIENCES
Field: Measurement (Instruments)
Classification: CPC G | IPC G

Claim 1:
2. The time-resolved fluorescence kit according to claim 1, wherein the europium-labeled anti-4,15-diacetoxyscirpenol monoclonal antibody is prepared by using the following method: adding the anti-4,15-diacetoxyscirpenol monoclonal antibody according to a mass ratio of an europium labeling reagent to the anti-4,15-diacetoxyscirpenol monoclonal antibody being 1:0.04-0.3, oscillating on a shaking table for 2 h to 4 h, centrifuging to completely discard supernate, and blocking redundant binding sites on a surface of the europium labeling reagent to obtain the target product, namely the europium-labeled anti-4,15-diacetoxyscirpenol monoclonal antibody;
the europium-labeled anti-aflatoxin B1 monoclonal antibody is prepared by using the following method: adding a proper amount of the anti-aflatoxin B1 monoclonal antibody according to a mass ratio of an europium labeling reagent to the anti-aflatoxin B1 monoclonal antibody being 1:0.04-0.3, oscillating on a shaking table for 2 h to 4 h, centrifuging to completely discard supernate, and blocking redundant binding sites on a surface of the europium labeling reagent to obtain the target product, namely the europium-labeled anti-aflatoxin B1 monoclonal antibody; and
the europium-labeled anti-sterigmatocystin monoclonal antibody is prepared by using the following method: adding a proper amount of the anti-sterigmatocystin monoclonal antibody according to a mass ratio of an europium labeling reagent to the anti-sterigmatocystin monoclonal antibody being 1:0.04-0.3, oscillating on a shaking table for 2 h to 4 h, centrifuging to completely discard supernate, and blocking redundant binding sites on a surface of the europium labeling reagent to obtain the target product, namely the europium-labeled anti-sterigmatocystin monoclonal antibody.