Patent ID: 11904317
Assignee: HIFIBIO SAS
Field: Measurement (Instruments)
Classification: CPC B  G | IPC B  G

Claim 0:
1. A method for the detection of a compound of interest in a microfluidic system, said method comprising:
a. creating, in said microfluidic system, at least one droplet capable of generating a detectable event, said at least one droplet comprising:
i. at least one single cell;
ii. one or more first capturing agent, wherein said one or more first capturing agent is capable of binding said at least one single cell as well as said compound of interest; and
iii. one or more second capturing agent comprising a label, wherein said one or more second capturing agent is capable of binding said compound of interest;

b. incubating said at least one droplet; and
c. subjecting said at least one droplet to a direct detection; wherein the presence or relocalization of said detectable event within said at least one droplet is indicative of the presence of said compound of interest, wherein the compound of interest is produced by said at least one single cell, and wherein said at least one or more first capturing agent is bound to said at least one single cell before said at least one droplet is formed.