Patent ID: 11913054
Assignee: BASF SE
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 0:
1. A method for fermentative production of n-butylacrylate (n-BA) comprising the steps of:
i) providing a recombinant microorganism comprising a butanol producing pathway and an acryloyl-CoA producing pathway and expressing an alcohol acyl transferase (AAT) gene encoding an AAT enzyme having an n-BA forming activity;
ii) culturing said microorganism under conditions that allow for the production of n-BA; and
iii) recovering n-BA from a fermentation broth;
wherein the AAT gene encoding an AAT enzyme having an n-BA forming activity is selected from the group consisting of:
(I) a nucleic acid molecule comprising a sequence selected from the group consisting of SEQ ID NO: 1, 3, 5, 7, 9, 11 and 13;
(II) a nucleic acid molecule having at least 95% identity to a nucleic acid molecule selected from the group consisting of SEQ ID NO: 1, 3, 5, 7, 9, 11 and 13;
(III) a nucleic acid molecule hybridizing to the full complement of a nucleic acid molecule having a sequence selected from the group consisting of SEQ ID NO: 1, 5, 7, 9, 11 and 13;
(IV) a nucleic acid molecule encoding a polypeptide selected from the group consisting of SEQ ID NO: 2, 6, 8, 10, 12, and 14; and
(V) a nucleic acid molecule encoding a polypeptide having at least 95% identity to a polypeptide selected from the group consisting of SEQ ID NO: 2, 6, 8, 10, 12, and 14.