Patent ID: 11873323
Assignee: OXFORD UNIVERSITY INNOVATION LIMITED
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 9:
10. A nucleic acid molecule comprising a nucleotide sequence which encodes a polypeptide comprising:
i) an amino acid sequence as set forth in SEQ ID NO: 2; or
ii) a portion of (i) comprising an amino acid sequence as set forth in SEQ ID NO: 101; or
iii) an amino acid sequence with at least 80% sequence identity to a sequence as set forth in SEQ ID NO: 2, wherein said amino acid sequence comprises a lysine at position 34, a glutamic acid at position 80 and two or more of the following:
1) threonine at position 5;
2) proline at position 16;
3) arginine at position 40;
4) histidine at position 65;
5) proline at position 92;
6) aspartic acid at position 100:
7) glutamic acid at position 108; or
8) threonine at position 116,, wherein the specified amino acid residues are at positions equivalent to the positions in SEQ ID NO: 2; or
iv) a portion of (iii) comprising an amino acid sequence with at least 80% sequence identity to a sequence as set forth in SEQ ID NO: 101, wherein the amino acid sequence comprises a lysine at position 10, a glutamic acid at position 56 and one or more of the following:
1) arginine at position 16;
2) histidine at position 41;
3) proline at position 68; or
4) aspartic acid at position 76,, wherein the specified amino acid residues are at positions equivalent to the positions in SEQ ID NO: 101,, and wherein said polypeptide is capable of spontaneously forming an isopeptide bond with a peptide comprising an amino acid sequence as set forth in SEQ ID NO: 5, wherein said isopeptide bond forms between the aspartic acid residue at position 10 of SEQ ID NO: 5 and the lysine residue at position 34 of SEQ ID NO: 2 or position 10 of SEQ ID NO: 101.