Patent ID: 11892446
Assignee: KING ABDULLAH UNIVERSITY OF SCIENCE AND TECHNOLOGY
Field: Measurement (Instruments)
Classification: CPC G | IPC G

Claim 7:
8. A method for assaying cell adhesion or cell rolling of multiple cell specimens comprising:
(a) contacting each cell specimen with a primary binding agent that specifically binds to an epitope on the surface of cells in the cell specimen;
(b) contacting each cell specimen with a second binding agent conjugated to a fluorescent label, wherein each cell specimen is contacted with a second binding agent comprising a different fluorescent label and the second binding agent specifically binds to the primary agent bound to an epitope on the surface of cells in the cell specimen;
(c) perfusing the cell specimens contacted with the second binding agents through a parallel plate flow chamber comprising a monolayer of endothelial cells;
(d) exciting the fluorescent label with an amount of electromagnetic energy; and
(e) capturing real-time fluorescence images of the multiple cell specimens as the multiple cell specimens pass through the parallel plate flow chamber, wherein the monolayer of cells are genetically engineered to express an epitope specifically bound by cells in the cell specimens.