Patent ID: 11857616
Assignee: INSTITUT PASTEUR
Field: Pharmaceuticals (Chemistry)
Classification: CPC A  C  G  Y | IPC A  C

Claim 0:
1. A nucleic acid construct which comprises:
(1) a polynucleotide encoding at least (i) the precursor of membrane (prM) protein of a Zika virus (ZIKV), and the envelope (E) protein of a ZIKV or a truncated version thereof of SEQ ID NO: 26, SEQ ID NO: 29 and SEQ ID NO: 32, or (ii) the E protein of a ZIKV or the truncated version thereof of SEQ ID NO: 26, SEQ ID NO: 29 and SEQ ID NO: 32; and
(2) a cDNA molecule encoding a full-length, infectious antigenomic (+) RNA strand of a live-attenuated measles virus (MV) vaccine strain;
wherein the polynucleotide encoding at least (i) the prM protein of the ZIKV, and the E protein of the ZIKV or the truncated version thereof, or (ii) the E protein of the ZIKV or the truncated version thereof and the cDNA molecule are operatively linked;
the nucleic acid construct comprising the following polynucleotides from 5′ to 3′:
(a) a polynucleotide encoding the N protein of the MV;
(b) a polynucleotide encoding the P protein of the MV;
(c) the polynucleotide encoding at least (i) the prM protein of the ZIKV, and the E protein of the ZIKV or the truncated version thereof, or (ii) the E protein of the ZIKV or the truncated version thereof;
(d) a polynucleotide encoding the M protein of the MV;
(e) a polynucleotide encoding the F protein of the MV;
(f) a polynucleotide encoding the H protein of the MV; and
(g) a polynucleotide encoding the L protein of the MV;

wherein said polynucleotides are operably linked in the nucleic acid construct and under a control of viral replication and transcription regulatory sequences.