Patent ID: 11919006
Assignee: INDIAN INSTITUTE OF TECHNOLOGY, KHARAGPUR
Field: Measurement (Instruments)
Classification: CPC B  G  C | IPC B  C  G

Claim 15:
16. The method as claimed in claim 1, wherein the step (II) of dispensing through a seamless fluidic pathway the RT LAMP-based hybridized reaction products and the single labelled free probes onto the cooperatively disposed POC colorimetric detector further comprises: (a) pushing forward the enclosed reaction microchambers to a dispensing position and allowing the microchambers to cool down to room temperature; (b) aligning the enclosed reaction microchambers with said POC colorimetric detector; and (c) dispensing the RT LAMP-based hybridized reaction products and the single labelled free probes from said reaction microchamber to flow through said functionalized microfluidic paper substrate/strip based POC colorimetric detector as a lateral flow assay strip for complementary analyte binding/hybridized reaction product generating colorimetric changes in said microfluidic paper substrate for detection, wherein the surface plasmon nanomaterial conjugated anti-fluorescein amidite antibody binds with the targeted dual labelled DNA and the conjugate-DNA complex which favors producing the color by the concentration of the colloidal nanomaterials to enable the detection involving said imaging using a smartphone device.