Patent ID: 11926823
Assignee: PIONEER HI-BRED INTERNATIONAL, INC.
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 8:
9. A wheat plant having modified male-fertility comprising at least one altered target site that confers modified male-fertility to the wheat plant, wherein the at least one altered target site originated from a corresponding target site that was recognized and cleaved by a guideRNA/Cas endonuclease system, and wherein the at least one altered target site is located in or near an endogenous wheat male fertility gene of MS9, MS22, MS26, or MS45, wherein the endogenous wheat male fertility gene of Ms9 comprises a wheat Ms9 polynucleotide sequence selected from the group consisting of: (a) a polynucleotide comprising the sequence set forth in SEQ ID NO:1-3, 5-7, 9-11, or 13-15; (b) a polynucleotide having at least 85%, 90% or 95% sequence identity to SEQ ID NO: 1-3, 5-7, or 9-11; (c) a polynucleotide that encodes a polypeptide having at least 85%, 90% or 95% sequence identity to SEQ ID NO: 4, 8, or 12; (d) a polynucleotide that encodes a polypeptide of SEQ ID NO: 4, 8, or 12; wherein the endogenous wheat male fertility gene of Ms22 comprises a wheat Ms22 polynucleotide sequence selected from the group consisting of: (a) a polynucleotide comprising the sequence set forth in SEQ ID NO:21-23, 25-27, or 29-31; (b) a polynucleotide having at least 85%, 90% or 95% sequence identity to SEQ ID NO: 21-23, 25-27, or 29-31; (c) a polynucleotide that encodes a polypeptide having at least 85%, 90% or 95% sequence identity to SEQ ID NO:24, 28, or 32; and (d) a polynucleotide that encodes a polypeptide of SEQ ID NO: 24, 28, or 32; wherein the endogenous wheat male fertility gene of Ms26 comprises a wheat Ms26 polynucleotide sequence selected from the group consisting of: (a) a polynucleotide comprising the sequence set forth in SEQ ID NO:41-43, 45-47, or 49-51; (b) a polynucleotide having at least 85%, 90% or 95% sequence identity to SEQ ID NO: 41-43, 45-47, or 49-51; (c) a polynucleotide that encodes a polypeptide having at least 85%, 90% or 95% sequence identity to SEQ ID NO:44, 48, or 52; and (d) a polynucleotide that encodes a polypeptide of SEQ ID NO: 44, 48, or 52; and wherein the endogenous wheat male fertility gene of Ms45 comprises a wheat Ms45 polynucleotide sequence selected from the group consisting of: (a) a polynucleotide comprising the sequence set forth in SEQ ID NO:61-63, 65-67, or 69-71; (b) a polynucleotide having at least 85%, 90% or 95% sequence identity to SEQ ID NO: 61-63, 65-67, or 69-71; (c) a polynucleotide that encodes a polypeptide having at least 85%, 90% or 95% sequence identity to SEQ ID NO:64, 68, or 72; and (d) a polynucleotide that encodes a polypeptide of SEQ ID NO: 64, 68, or 72, and the altered target site affects the expression level of the MS9, MS22, MS26, or MS45 gene so that the wheat plant is male-sterile.