Patent ID: 11951155
Assignee: ZHONGSHI DUQING(SHANDONG) BIOTECH CO., LTD
Field: Pharmaceuticals (Chemistry)
Classification: CPC A | IPC A

Claim 2:
3. The method according to claim 1, wherein in step 5), the separation and purification by preparative liquid chromatography is specifically conducted using a preparative liquid chromatographic column at a predetermined flow rate using a mobile phase A comprising a trifluoroacetic acid aqueous solution, and a mobile phase B comprising a trifluoroacetic acid acetonitrile solution; and a gradient elution process including the steps of:
eluting from 0 min to 10 min, with a volume percentage of the mobile phase A decreased from 100% to 90%, and a volume percentage of the mobile phase B increased from 0 to 10%;
eluting from 10 min to 20 min, with a volume percentage of the mobile phase A decreased from 90% to 50%, and a volume percentage of the mobile phase B increased from 10% to 50%;
eluting from 20 min to 60 min, with a volume percentage of the mobile phase A decreased from 50% to 10%, and a volume percentage of the mobile phase B increased from 50% to 90%;
eluting from 60 min to 65 min, with a volume percentage of the mobile phase A increased from 10% to 95%, and a volume percentage of the mobile phase B decreased from 90% to 5%; and
eluting from 65 min to 100 min, with a volume percentage of the mobile phase A at 95%, and a volume percentage of the mobile phase B at 5%.