Patent ID: 11857961
Assignee: MIROCULUS INC.
Field: Chemical engineering (Chemistry)
Classification: CPC B  C | IPC B  C

Claim 0:
1. A method of sequencing by synthesis using mechanical compression, the method comprising:
sequencing a library of polynucleotide fragments by:
sequentially moving each droplet of a series of fluidic droplets within a cartridge, wherein the cartridge comprises an air gap formed between a first sheet having a first surface that is hydrophobic and oleophobic and a second sheet having a second surface that is hydrophobic and oleophobic, wherein the first sheet and the second sheet are secured opposite each other with the air gap therebetween, the cartridge further comprising the library of polynucleotide fragments, the library of polynucleotide fragments comprising a plurality of clusters of polynucleotides on the first sheet or the second sheet, wherein moving each droplet comprises moving one or more mechanical force applicators against the first sheet to elastically deform the first sheet and create a region of reduced spacing between the first and second sheets so that each droplet follows the region of reduced spacing over the plurality of clusters of polynucleotides, wherein the series of fluidic droplets includes: a nucleotide polymerase reaction mix droplet, a first wash buffer droplet, a dye/terminator cleavage mix droplet, and a second wash buffer droplet;
imaging the plurality of clusters of polynucleotides to detect addition or hybridization of a nucleotide to clusters of the plurality of clusters of polynucleotides; and
repeating the steps of sequentially moving each droplet of the series of fluidic droplets and imaging the plurality of clusters to generate sequence data for the plurality of clusters of polynucleotides.