Patent ID: 11959061
Assignee: nan
Field: Measurement (Instruments)
Classification: CPC C  G | IPC C  G

Claim 10:
11. A method for electroporation of biological cells, comprising:
providing a sample container for electroporation, the sample container having an insulator chamber for forming the body of the container to hold cells, a first electrode and a second electrode providing electrical connection for electroporation, wherein the insulator chamber includes an excessive-receiving groove on a top of one side of the insulator chamber, and the first electrode is in a cover configured to removably cover an inner rim of the excessive-receiving groove on a side of the insulator chamber to seal the insulator chamber;
filling the sample container with a sample containing biological cells in a volume slightly larger than the sealed volume in the sample container to form a convex meniscus surface in the insulator chamber;
closing down the first electrode on the inner rim to push out excess liquid and seal the chamber, wherein the excess liquid flows to the excess-receiving groove; and
delivering an electrical pulse to perform electroporation, wherein the excess liquid in the excess-receiving groove is discardable after electroporation.