Patent ID: 11939630
Assignee: SHAANXI LIFEGEN CO., LTD.
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 1:
2. The specific primer probe combination according to claim 1, further comprising specific primers and probes designed for an internal reference gene β-Actin, which are as follows:
forward primer ACTB-F, the nucleotide sequence of ACTB-F is set forth in SEQ ID NO: 6;
reverse primer ACTB-R, the nucleotide sequence of ACTB-R is set forth in SEQ ID NO: 7; and
probe ACTB-P: 5′-6-carboxy-fluoroscein-AGGTACTAACACTGGCTCGTGTGAC quenching group-3′, the nucleotide sequence of between 6-carboxy-fluorescein and quenching group is set forth in SEQ ID NO: 8;, wherein 6-carboxy-fluorescein is a fluorescent group, and the quenching group has a chemical structure of,