Patent ID: 11918702
Assignee: THE TRUSTEES OF COLUMBIA UNIVERSITY IN THE CITY OF NEW YORK
Field: Measurement (Instruments)
Classification: CPC A  C  G | IPC A  C  G

Claim 11:
12. A cell-based assay for identification of agents that treat a lung disease, comprising providing a control sample of one or more mutated BLBOs generated by the method of claim 6 and a test sample of one or more mutated BLBOs of claim 6; contacting the test sample with a test agent; determining the level of (a) expression of at least one lung and airway epithelial cell marker, (b) extracellular matrix deposition, (c) metabolism, (d) endosome trafficking, (e) cellular stress response, (f) unfolded protein response, (g) mitophagy, (h) autophagy, (i) surfactant secretion or (j) recycling, or a combination thereof, in the control and the test samples; and selecting the test agent if any determined level in the test sample is significantly different from the control sample.