Patent ID: 11892448
Assignee: HOFFMANN-LA ROCHE, INC.
Field: Measurement (Instruments)
Classification: CPC G | IPC G

Claim 2:
3. The method according to claim 1, comprising the following steps:
a) determining based on the result of an immunoassay the fraction of free drug in a sample comprising drug, target and drug-target-complexes for a first drug:target ratio in the sample
wherein the drug:target ratio is equal or less than a drug:target ratio for which the determined fraction of free drug is comparable for at least two different drug:target ratios,
b) determining based on the result of an immunoassay the fraction of free drug in a sample comprising drug, target and drug-target-complexes for at least a second drug:target ratio in the sample,
wherein the second drug:target ratio is different from the first drug:target ratio by increasing or reducing the amount of target in the sample and by maintaining the amount of drug in the sample,
wherein the determined fractions of free drug is comparable for the drug:target ratio with increased or reduced amount of target in the sample for an increased or reduced amount of drug in the sample and the starting drug:target ratio, and
c) calculating based on the fraction of free drug determined in the previous step the binding affinity of the drug to its target.