Patent ID: 11926857
Assignee: CODEXIS, INC.
Field: Biotechnology (Chemistry)
Classification: CPC C  Y | IPC C

Claim 2:
3. The engineered variant LovD polypeptide of claim 1, wherein said engineered variant LovD polypeptide further comprises:
(i) one or more substitutions that correlate with an increase in catalytic activity as compared to the wild-type Aspergillus terreus acyltransferase of the amino acid sequence of SEQ ID NO: 2;
(ii) one or more substitutions that correlate with increased thermal stability as compared to the wild-type Aspergillus terreus acyltransferase of the amino acid sequence of SEQ ID NO: 2;
(iii) one or more substitutions that correlate with reduced aggregation as compared to the wild-type Aspergillus terreus acyltransferase of the amino acid sequence of SEQ ID NO: 2;
(iv) one or more substitutions that correlate with increased enzyme stability as compared to the wild-type Aspergillus terreus acyltransferase of the amino acid sequence of SEQ ID NO: 2; and
(v) one or more substitutions selected from the group consisting of 14N, A9V, K26E, R28K, R28S, 135L, C40A, C40V, C40F, S41R, N43Y, C60F, C60Y, C60N, C60H, S109C, S142N, A184T, A184V, N191S, A261T, A261E, A261V, L292R, Q297E, L335M, A377V, A383V, N391D, and H404R.