Patent ID: 11958881
Assignee: ZHEJIANG UNIVERSITY
Field: Chemical engineering (Chemistry)
Classification: CPC C  B | IPC B  C

Claim 3:
4. The optimization method for capturing the proteins by the MCC according to claim 1, wherein a graphic method used to solve the optimal load residence time in step 4 comprises the following steps:
drawing the line tC-RTC in the t-RT coordinate system based on the linear relationship between the interconnected load time tC and the load residence time RTC obtained in step 2, and drawing the line of the third formula, t
  =
  
   
    
     t
     RR
    
    -
    
     
      (
      
       N
       -
       3
      
      )
     
     ⁢
     
      t
      CW
     
    
   
   
    (
    
     N
     -
     2
    
    ), in the t-RT coordinate system, wherein tCW (min) is the interconnected wash time of the MCC, tRR (min) is the R-R time of the MCC and comprises the sum of the washing time, the elution time, and the regeneration time, and N is the number of the operating columns; the load residence time corresponding to the intersection of the two lines is the optimal load residence time for capturing the proteins by the MCC under the set loading protein concentration, the set protein breakthrough percentage, and the optimal number of the operating columns.