Patent ID: 11959132
Assignee: BIOFIRE DIAGNOSTICS, LLC
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 7:
8. A method for analyzing a target nucleic acid in a sample comprising
(a) providing a sample container comprising a first blister and a second sample well, the second sample well comprising therein primers for amplifying the target nucleic acid, a control nucleic acid, and primers for amplifying the control nucleic acid,
(b) subjecting the sample to a first amplification in the first blister, and moving a portion of the sample from the first blister to the second sample well,
(c) subjecting the second sample well to amplification conditions through at least one amplification cycle to amplify the target nucleic acid and the control nucleic acid in the second sample well, wherein the amplification conditions include a dsDNA binding dye,
(d) generating a melt curve of the amplified target nucleic acid and amplified control nucleic acid,
(e) determining a value for the amplified target nucleic acid, and
(f) repeating steps (c), (d), and (e).