Patent ID: 11911455
Assignee: BEIJING AEONVITAL BIOMEDICINE RESEARCH CO., LTD.
Field: Pharmaceuticals (Chemistry)
Classification: CPC A  C  Y | IPC A  C

Claim 13:
14. A preparation method for amplifying the recombinant vector according to claim 12, characterized in that:
comprising the following steps:
1) Synthesizing four nucleic acid sequences of the vaccine sequences HPV16-AVLS1, HPV16-AVLC1, HPV18-AVLS1 and HPV18-AVLC1 by splicing the amino acid sequences of E6/E7 proteins, L1/L2 peptides, IgK, ubiquitin, PADRE, and adjuvant;
2) Inserting the above four nucleic acid sequences into the vector PUC57 using HindIII and XhoI restriction enzyme sites, and then subcloning the vaccine sequences into the expression vector AVL0318; obtaining four recombinant vectors AVL0318-HPV16/18-AVLS1/AVLC1;
3) Amplifying the plasmids of AVL0318-HPV16/18-AVLS1/AVLC1 using Escherichia coli AVL-DH5a;
wherein, the genome sequence of Escherichia coli AVL-DH5a contains the SacB gene for constitutive expression and does not contain antibiotic selection markers; the sequence capable of expressing the SacB gene is shown as SEQ ID No: 17.