Patent ID: 11970739
Assignee: 10X GENOMICS, INC.
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 0:
1. A composition comprising:
a) a spatial array comprising a substrate comprising:
(i) a first plurality of capture probes wherein the first plurality of capture probes comprise a spatial barcode and a first plurality of capture domains; and
(ii) a second plurality of capture probes wherein the second plurality of capture probes comprise a spatial barcode and a second plurality of capture domains hybridized to a plurality of oligonucleotides from an analyte capture agent, wherein the analyte capture agent comprises an analyte-binding moiety comprising an antibody or an antigen-binding fragment thereof, and wherein the oligonucleotides comprise an analyte capture sequence and an analyte binding moiety barcode; and

b) a pair of probes comprising:
(i) a plurality of first probes, wherein a first probe of the plurality of first probes is hybridized to a target nucleic acid and
(ii) a plurality of second probes, wherein a second probe of the plurality of second probes is hybridized to the target nucleic acid at a separate location from the first probe and wherein the second probe has a sequence complementary to the first plurality of capture domains.