Patent ID: 11965198
Assignee: AJINOMOTO CO., INC.
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 0:
1. A method for producing a target substance selected from the group consisting of an amino acid, a nucleoside, a nucleotide, an isoprenoid, and a peptide, comprising:
(i) cultivating in a culture medium a bacterium which has an ability to produce the target substance, wherein said bacterium is able to produce and accumulate the target substance in the culture medium or cells of the bacterium, or both, and
(ii) collecting the target substance from the culture medium or the cells, or both,, wherein the target substance is a substance from an ATP-dependent biosynthetic pathway, and, wherein the bacterium has been modified to overexpress a gene encoding a protein having H*-translocating membrane-bound pyrophosphatase activity; and, wherein the gene encoding the protein having H+-translocating membrane-bound pyrophosphatase activity is selected from the group consisting of:
(A) a DNA comprising the nucleotide sequence set forth in SEQ ID NO: 1,
(B) a DNA comprising a nucleotide sequence that hybridizes under stringent conditions to a nucleotide sequence complementary to the sequence set forth in SEQ ID NO: 1, and wherein the stringent conditions comprise washing at a salt concentration of 1×SSC, 0.1% SDS at 60° C., 0.1×SSC, 0.1% SDS at 60° C., or 0.1×SSC, 0.1% SDS at 65°,
(C) a DNA encoding a protein comprising the amino acid sequence set forth in SEQ ID NO: 2,
(D) a DNA encoding a protein comprising the amino acid sequence set forth in SEQ ID NO: 2, wherein the amino acid sequence comprises substitution, deletion, insertion, and/or addition of 1 to 70 amino acid residues and has H+-translocating membrane-bound pyrophosphatase activity,
(E) a DNA encoding a protein comprising an amino acid sequence at least 90% identical to the amino acid sequence set forth in SEQ ID NO: 2 and has H+-translocatinq membrane-bound pyrophosphatase activity, and
(F) a DNA comprising a variant nucleotide sequence set forth in SEQ ID NO: 1 due to the degeneracy of the genetic code.