Patent ID: 11913956
Assignee: SHANDONG ZEJI BIOTECHNOLOGY CO., LTD.
Field: Measurement (Instruments)
Classification: CPC G  C | IPC G

Claim 6:
7. An assay kit for tumor screening, evaluating risk of tumor development and identification of therapeutic efficacy of tumor treatment in subjects, comprising:
a reagent used to detect GAPDH in a blood sample, wherein an amino acid sequence of GAPDH is shown as SEQ ID No. 1;
wherein the reagent comprises a capture antibody and a detection antibody, the capture antibody binds to N-terminal amino acids 40-160 of the GAPDH amino acid sequence for capturing GAPDH from the blood sample, and the detection antibody binds to N-terminal amino acids 180-335 of the GAPDH amino acid sequence for measuring the concentration of GAPDH,
wherein the capture antibody comprises heavy chain variable region and light chain variable region, the heavy chain variable region comprises CDR1 shown as SEQ ID No. 2, CDR2 shown as SEQ ID No. 3 and CDR3 shown as SEQ ID No. 4, the light chain variable region comprises CDR1 shown as SEQ ID No. 5, CDR2 shown as SEQ ID No. 6 and CDR3 shown as SEQ ID No. 7, and
wherein the detection antibody comprises heavy chain variable region and light chain variable region, the heavy chain variable region comprises CDR1 shown as SEQ ID No. 8, CDR2 shown as SEQ ID No. 9 and CDR3 shown as SEQ ID No. 10, the light chain variable region comprises CDR1 shown as SEQ ID No. 11, CDR2 shown as SEQ ID No. 12 and CDR3 shown as SEQ ID No. 13.