Patent ID: 11891632
Assignee: GENECAST CO., LTD
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 7:
8. The Taq polymerase mutant of claim 7, wherein the Taq polymerase mutant is used for discriminating a matched primer from a mismatched primer by comparing a Ct value of a quantitative polymerase chain reaction (qPCR) in the presence of a nucleic acid template and the matched primer with a Ct value of a qPCR in the presence of the nucleic acid template and the mismatched primer, wherein both the matched primer and the mismatched primer are capable of hybridizing with a target sequence of the nucleic acid template, and the mismatched primer has a non-complementary nucleotide at its 3′ end after it hybridizes to the target sequence of the nucleic acid template;
wherein the matched primer consists of a nucleotide sequence of SEQ ID NO: 43 and the mismatched primer consists of a nucleotide sequence of SEQ ID NO: 44 when the nucleic acid template is a KRAS gene comprising Q61H variation, or the matched primer consists of a nucleotide sequence of SEQ ID NO: 47 and the mismatched primer consists of a nucleotide sequence of SEQ ID NO: 48 when the nucleic acid template is a KRAS gene comprising Q61H variation; or
wherein the matched primer consists of a nucleotide sequence of SEQ ID NO: 51 and the mismatched primer consists of a nucleotide sequence of SEQ ID NO: 52 when the nucleic acid template is a KRAS gene comprising G13D variation; or
wherein the matched primer consists of a nucleotide sequence of SEQ ID NO: 54 and the mismatched primer consists of comprises a nucleotide sequence of SEQ ID NO: 55 when the nucleic acid template is a KRAS gene comprising G12S variation; or
wherein the matched primer consists of a nucleotide sequence of SEQ ID NO: 59 and the mismatched primer consists of a nucleotide sequence of SEQ ID NO: 60 when the nucleic acid template is a EGFR gene comprising L585R variation.