Patent ID: 11898165
Assignee: MINK THERAPEUTICS, INC.
Field: Measurement (Instruments)
Classification: CPC C  A  G | IPC C  G

Claim 2:
3. The isolated cell of claim 1, wherein:
(a) the isolated cell is derived from a single cell and wherein when the cell is expanded to a plurality of cells of at least I 05 and transduced or transfected with separate polynucleotide vectors encoding a TCR α chain and a TCR β chain to express a recombinant TCR comprising the TCR α chain and the TCR β chain under conditions that maximize transduction or transfection efficiency, at least 20% of the plurality of cells express the recombinant TCR on the cell surface, optionally the cell has previously been subjected to one or more rounds of transduction or transfection;
(b) the mouse lymphocyte is a T cell, B cell, or an NK cell;
(c) the mouse lymphocyte is a T cell that is selected from the group consisting of a CD8+ cytotoxic T cell, a CD8+ regulatory T cell, a CD4+ cytotoxic T cell, a CD4+ helper T cell, and a CD4+ regulatory T cell; and/or
(d) the mouse lymphocyte is selected from the group consisting of a RAG1 or RAG2 knockout murine cell, an immature murine T cell, a mouse DO-11.10.7 58 α−β− (5831 /−) cell, and a mouse Bw5147 cell.