Patent ID: 11859087
Assignee: SUZHOU BAIYUAN GENT CO., LTD.
Field: Basic materials chemistry (Chemistry)
Classification: CPC C | IPC C

Claim 13:
14. The method of preparing fluorescence-encoded microspheres according to claim 13, wherein the intermediate (I′) is prepared by the steps of:
(1) preparing an intermediate I-1
adding phenylhydrazine to glacial acetic acid with stirring, and then slowly adding dropwise 3-methyl-2-butanone, heating to 60-65° C. for reaction after completing addition of 3-methyl-2-butanone to obtain a reaction product, followed by extracting, concentrating, and refining the reaction product to obtain the intermediate I-1;
(2) preparing an intermediate I-2
adding the intermediate I-1 and 1,2-dibromoethylene into toluene, then heating to reflux in the presence of nitrogen to obtain a reaction product, followed by cooling to precipitate a solid to obtain the intermediate I-2;
(3) preparing an intermediate I-4
adding dry N,N-dimethylformamide to dry dichloromethane, and adding a solution of phosphorus oxychloride in dichloromethane and cyclohexanone in an ice bath, and then removing the ice bath, followed by heating to reflux to obtain a reaction solution, then pouring the reaction solution into crushed ice, left standing overnight to precipitate a solid to obtain the intermediate I-4; and
(4) preparing the intermediate I′
adding the intermediate I-2 and the intermediate I-4 to a mixture of n-butanol and toluene, heating to reflux and precipitating a solid filtered to obtain the intermediate (I′);,