Patent ID: 11890301
Assignee: WAYNE STATE UNIVERSITY
Field: Pharmaceuticals (Chemistry)
Classification: CPC C  A | IPC A  C

Claim 0:
1. A method of metabolically enhancing a tumor specific T cell, comprising:
introducing a first nucleic acid encoding a chimeric antigen receptor (CAR) into a T cell in vitro to obtain a CAR T cell, wherein the CAR comprises an antigen binding domain that binds to CD19, a transmembrane domain, and intracellular signaling domains of a 4-1BB molecule and a CD3zeta molecule;
arming the CAR T cell in vitro with a bispecific antibody, wherein arming the CAR T cell comprises introducing a second nucleic acid encoding the bispecific antibody into the CAR T cell, wherein the bispecific antibody binds CD3 on the CAR T cell, and wherein the bispecific antibody is capable of binding to an antigen on a tumor cell in vivo; and
administering the armed CAR T cell to a CD19+ subject wherein the intracellular signaling domain is activated, thereby metabolically enhancing the tumor specific T cell.