Patent ID: 11884705
Assignee: JSR LIFE SCIENCES CORPORATION
Field: Chemical engineering (Chemistry)
Classification: CPC B  C  G | IPC B  C  G

Claim 7:
8. A method for producing a mutant of an immunoglobulin binding domain, the method comprising:
providing a polypeptide consisting of the amino acid sequence set forth in SEQ ID NO: 9 or an amino acid sequence having an identity of at least 95% therewith,
introducing into the polypeptide at least one mutation selected from the group consisting of substitution of lysine at a position corresponding to the 26th position of the amino acid sequence set forth in SEQ ID NO:9 with arginine, substitution of glycine at a position corresponding to the 34th position of the amino acid sequence set forth in SEQ ID NO:9 with histidine, and optionally at least one of substitution of threonine at a position corresponding to the 35th position of the amino acid sequence set forth in SEQ ID NO:9 with arginine, substitution of glycine at a position corresponding to the 42nd position of the amino acid sequence set forth in SEQ ID NO:9 with lysine, and substitution of asparagine at a position corresponding to the 54th position of the amino acid sequence set forth in SEQ ID NO:9 with glutamine, to produce a mutant immunoglobulin binding domain having immunoglobulin κ chain binding activity.