Patent ID: 11939598
Assignee: ELIGO BIOSCIENCE
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 0:
1. A method for producing phage particles or phage-derived delivery vehicles, comprising:
(a) providing a production bacterial cell stably comprising phage structural genes and phage DNA packaging genes of a first type of bacteriophage,
wherein the expression of said phage structural genes and phage DNA packaging genes in said production bacterial cell is controlled by an induction mechanism comprising phage excision/insertion genes, phage DNA replication genes, and phage regulation genes of a second, different type of bacteriophage, wherein said phage excision/insertion genes, phage DNA replication genes and phage regulation genes are neither phage DNA packaging genes nor phage structural genes,
wherein said production bacterial cell does not comprise phage excision/insertion genes and/or phage replication genes of the first type of bacteriophage,
wherein said production bacterial cell is from a bacterial species or strain different from the bacterial species or strain from which said first type of bacteriophage comes from and/or that said first type of bacteriophage targets and wherein said production bacterial cell is from the same bacterial species or strain as the bacterial species or strain from which said second type of bacteriophage comes from and/or that said second type of bacteriophage targets,
wherein said production bacterial cell is a P. freudenreichii bacterial cell,
wherein the first type of bacteriophage is a C. acnes phage and
wherein the second type of bacteriophage is a P. freudenreichii phage;

and
(b) inducing, in said production bacterial cell, expression of said phage structural genes and phage DNA packaging genes, and assembly of the products expressed by said phage structural genes and phage DNA packaging genes, thereby producing phage particles or phage-derived delivery vehicles.