Patent ID: 11959904
Assignee: PRESIDENT AND FELLOWS OF HARVARD COLLEGE
Field: Measurement (Instruments)
Classification: CPC G  B  C | IPC B  C  G

Claim 23:
24. A method for sensing translocation of a molecule through a nanopore comprising:
directing to an inlet of the nanopore at least one molecule in a first fluidic solution in a first fluidic reservoir, said first fluidic reservoir being disposed in fluidic connection with the inlet of the nanopore;
translocating at least a section of the molecule in the first fluidic solution through the nanopore and then into a fluidic passage disposed in fluidic connection with the nanopore, said fluidic passage including a passage length having a fluidic passage fluidic resistance, RFP, of at least about 10% of a nanopore fluidic resistance, RPore, of the nanopore, and no more than about 10 times the nanopore fluidic resistance, RPore, and having a passage width that is greater than a diameter of the nanopore and that is less than said passage length; and
sensing an electrical potential local to the fluidic passage during translocation of said at least a section of the molecule through the nanopore, to sense translocation of said at least a section of the molecule.