Patent ID: 11927595
Assignee: FUTURE DIAGNOSTICS B.V.
Field: Measurement (Instruments)
Classification: CPC G | IPC G

Claim 0:
1. A method for assaying a sample of blood or blood components for the presence of free vitamin D comprising:
(a) adding an immobilized binding protein or antibody for 25-OH vitamin D to the sample;
(b) mixing the sample with a diluent, said diluent comprising a fluoroalkyl surfactant;
(c) incubating the sample for an effective amount of time to allow a desired amount of vitamin D to bind to the immobilized binding protein or antibody;
(d) removing non-bound serum and serum components by washing;
(e) subjecting the immobilized binding protein or antibody comprising captured vitamin D bound thereto to competitive binding with a labeled vitamin D compound;
(f) determining the concentration of labeled vitamin D compound bound to the binding protein,
wherein the fluoroalkyl surfactant does not dissociate vitamin D from vitamin D binding protein and albumin, and wherein the concentration of the surfactant is 0.05 to 0.5% by weight.