Patent ID: 11898204
Assignee: KAPA BIOSYSTEMS, INC.
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 0:
1. A method of separately sequencing each strand of a target nucleic acid comprising the steps of:
a) in a reaction mixture, joining the ends of a double stranded target nucleic acid to adaptors to form a doubly-adapted target nucleic acid wherein the adaptor is a single strand forming a double stranded stem and a single-stranded loop and the stem comprises at least one strand cleavage site;
b) contacting the reaction mixture with an exonuclease thereby enriching for the doubly-adapted target nucleic acid;
c) contacting the reaction mixture with a cleavage agent to cleave the doubly-adapted target nucleic acid at the cleavage sites forming extendable termini on each strand;
d) extending the extendable termini thereby separately sequencing each strand of the target nucleic acid, wherein extension is terminated at the cleavage site and does not proceed onto the complementary strand.