Patent ID: 11866781
Assignee: PRESIDENT AND FELLOWS OF HARVARD COLLEGE
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 0:
1. A method of analyzing nucleic acid sequences present in a sample, the method comprising:
(a) attaching a unique barcode sequence to substantially each of a plurality of DNA molecules in a sample to produce a plurality of differently barcoded DNA molecules, wherein each barcoded DNA molecule comprises a unique barcode sequence associated with the nucleic acid sequence of the DNA molecule;
(b) amplifying the plurality of differently barcoded DNA molecules in the sample to produce amplicons of the plurality of differently barcoded DNA molecules;
(c) sequencing each amplicon to generate sequence reads, wherein each sequence read comprises the sequence of the DNA molecule and the sequence of the associated unique barcode;
(d) sorting the sequence reads into groups, each group corresponding to a DNA molecule, wherein the sequence reads in each group comprise the same unique barcode sequence, wherein the sorting is performed by a computer implemented method;
(e) comparing two or more sequence reads within the same group.