Patent ID: 11932882
Assignee: 10X GENOMICS, INC.
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 8:
9. The engineered reverse transcriptase of claim 1, wherein said engineered reverse transcriptase exhibits an altered reverse transcriptase related activity as compared to a reverse transcriptase having the amino acid sequence set forth in SEQ ID NO:1; and wherein the altered reverse transcriptase related activity is selected from:
(a) an increased ability to yield median UMI counts per cell;
(b) an increased transcription efficiency;
(c) an increased template switching (TSO) efficiency;
(d) a decreased template switching (TSO) efficiency;
(e) an increased ability to yield UMI reads from genes of a desired length in a 3′ reverse transcription assay;
(f) an increased ability to yield UMI reads from genes of a desired length in a 5′ reverse transcription assay;
(g) an increased ability to yield median UM is/cell; and/or
(h) an increased transcription efficiency and an increased template switching efficiency.