Patent ID: 11926877
Assignee: THE BOARD OF TRUSTEES OF THE UNIVERSITY OF ILLINOIS
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 0:
1. A method of detecting viral coronavirus polynucleotides in a saliva sample from a subject comprising:
heating the biological sample from the subject at about 95 degrees Celsius for about 15 minutes to about 30 minutes;
contacting the saliva sample with one or more buffering agents and one or more non-ionic detergents to provide a test sample, wherein the saliva sample and the one or more buffering agents are present in a ratio of about 1:3 w/w to about 3:1 w/w wherein the one or more buffering agents comprise tris-borate-ethylenediamine tetraacetate (TBE) or tris-ethylenediamine tetraacetate (TE) and the one or more non-ionic detergents are present in the test sample in a concentration of about 0.25% to about 1% by weight; and
subjecting the test sample to conditions that amplify target viral coronavirus polynucleotides in the test sample using a polymerase chain reaction (PCR), and detecting the viral coronavirus polynucleotides in saliva sample.