Patent ID: 11920137
Assignee: AGENCY FOR SCIENCE, TECHNOLOGY AND RESEARCH
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 0:
1. A method for producing a-ionone comprising the step of expressing in an Escherichia coli host cell an expression module comprising an expression vector having a coding region encoding optimized a-ionone generating enzymes, the coding region being operably linked to a promoter,
wherein the optimized a-ionone generating enzymes are LcyE and CCD1 that form an operon having the structure of LcyE-CCD1, and
wherein the LcyE is obtained from Lactuca sativa and comprises an N-terminal truncation of 50 amino acids (ΔN50-LsLcyE), and
wherein the CCD1 is fused with an N-terminal fusion partner.