Patent ID: 11879019
Assignee: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA
Field: Pharmaceuticals (Chemistry)
Classification: CPC C  A  G | IPC A  C

Claim 13:
14. A method for controlled releasing an active ingredient, wherein the method comprising the steps of
(1) contacting a compound having a general structure of, , wherein X is selected from O, NH or S; R1 is an enzyme substrate, wherein R1 is a protease substrate selected from the group consisting of a chymotrypsin substrate, a papain substrate, a caspase 8 substrate and a caspase 3 substrate; R2 comprises an active ingredient; and R3 is selected from a protecting group, a H, an amino acid or a peptide; with a first enzyme, wherein R1 is cleaved from the compound upon digestion by the first enzyme to form a second compound with a free ε-amine group; and
(2) contacting the second compound with a second enzyme, wherein the active ingredient is released from the second compound upon digestion of the second enzyme—wherein the enzyme-responsive peptide requires digestion by a first enzyme selected from the group consisting of chymotrypsin, a papain, caspase 8 and caspase 3 and by a second enzyme, wherein the second enzyme is trypsin.