Patent ID: 11898139
Assignee: OKAYAMA PREFECTURAL GOVERNMENT
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 2:
3. A method for inhibiting an increase in viscosity of a culture solution of Rhizobium bacteria and improving viable cell count of the Rhizobium bacterium in the culture solution, the method comprising:
culturing a Rhizobium bacterium by liquid culture using a medium comprising one or more carbon sources, wherein a disaccharide accounts for at least 25 mass % of the one or more carbon sources in the medium while maintaining a pH of 5.0 to 7.0 in a culture solution from a beginning of the culturing to an end of the culturing; and
concentrating and/or separating viable bacteria from the culture solution,
wherein the disaccharide is at least one selected from the group consisting of maltose, trehalose, and lactose, and
wherein the Rhizobium bacterium is a Rhizobium vitis ARK-1 strain with accession number FERM BP-11426, a Rhizobium vitis ARK-2 strain with accession number FERM BP-11427, a Rhizobium vitis ARK-3 strain with accession number FERM BP-11428, or an Agrobacterium radiobacter K84 strain.