Patent ID: 11903969
Assignee: THE BOARD OF TRUSTEES OF THE LELAND STANFORD JUNIOR UNIVERSITY
Field: Pharmaceuticals (Chemistry)
Classification: CPC C  A | IPC A  C

Claim 0:
1. A method of treating leukemia in a subject that has previously received a hematopoietic stem cell transplant from an HLA-matched donor, the method comprising:
i) introducing into a plurality of αβ T cells from the donor a guide RNA targeting the TRAC locus, an RNA-guided nuclease, and a homologous repair template comprising a polynucleotide encoding a CD19-specific chimeric antigen receptor (CAR), wherein the RNA-guided nuclease cleaves the endogenous T cell receptor antigen constant (TRAC) locus in the plurality of cells and the polynucleotide encoding the CD19-specific CAR is integrated in-frame into the cleaved TRAC locus;
ii) expanding the modified T cells in culture to generate a population of modified CAR T cells; and
iii) administering the modified CAR T cells to the subject.