Patent ID: 11926858
Assignee: GLYCOM A/S
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 3:
4. The genetically modified microorganism of claim 1, wherein an aglycon in the glycoside of a recombinant oligosaccharide or a carbohydrate acceptor is selected from the group consisting of:
—ORA, wherein RA is a linear or branched hydrocarbon chain having, when saturated, 1-24 carbon atoms or, when unsaturated, 2-24 carbon atoms, or RA means an aryl moiety, or RA means a group removable by hydrogenolysis,
—X—RB, wherein X is N or S, and RB means linear or branched hydrocarbon chain having, when saturated, 1-24 carbon atoms or, when unsaturated, 2-24 carbon atoms, or RB means an aryl moiety, or RB means a benzyl group,
a group that links an anomeric carbon atom and an adjacent carbon atom to each other by a —NH—C(═O)—O-bridge, thus forming a fused 5-membered ring, as depicted in case of an aldose:, azide,
—NH—C(R″)═C(R′)2, wherein each R′ independently is an electron withdrawing group selected from —CN, —COOH, —COO-alkyl, —CO-alkyl, —CONH2, —CONH-alkyl and —CON(alkyl)2, or wherein the two R′-groups are linked together and form —CO—(CH2)2-4—CO— and thus form with the carbon atom, to which they are attached, a 5-7 membered cycloalkan-1,3-dione, in which dione any of its methylene groups is optionally substituted with 1 or 2 alkyl groups, and wherein R″ is H or alkyl,
a residue of an amino acid,
a polyethylene glycol residue,
a polyvinyl alcohol residue, and
an α,β-unsaturated amido group of formula A or an α,β-unsaturated carbonyl group of formula B, , wherein Q2, Q3 and Q4 are, independently, H and C1-C6-alkyl, which alkyl is optionally substituted with halogen, OH, nitro or phenyl groups.