Patent ID: 11891647
Assignee: NATIVE MICROBIALS, INC.
Field: Computer technology (Electrical engineering)
Classification: CPC C  G | IPC C  G

Claim 5:
6. A method, comprising:
obtaining at least two sample sets, each set having a plurality of samples;
for an identified sample from a plurality of samples in a sample set, detecting the presence of a plurality of microorganism strains;
identifying, by sequencing DNA in the identified sample, DNA representing whole genomes of the plurality of microorganism strains;
obtaining a set of sequencing reads associated with the plurality of microorganism strains from sequencing the DNA in the identified sample;
comparing each sequencing read from the set of sequencing reads with each DNA representing a whole genome in the set of DNA representing whole genomes to identify a match between that sequencing read and a DNA representing a whole genome;
determining, based on the comparing, a relative frequency of occurrence for each microorganism strain within the identified sample;
determining an absolute cell count of each microorganism strain in each sample from the total quantity of each microorganism type and the relative frequency of occurrence for each microorganism strain;
measuring RNA for each microorganism strain by sequencing cDNA in each sample and mapping the sequenced cDNA to the set of DNA representing whole genomes;
generating a gene expression profile identifying expression levels associated with a set of genes associated with each microorganism strain based on the RNA for each microorganism;
determining an activity for each microorganism strain in the identified sample based on the RNA for each microorganism strain;
identifying active microorganism strains in each sample, based on the activity for each microorganism strain in the identified sample;
identifying one or more pathways based on the gene expression profile associated with each microorganism strain from the active microorganism strains; and
subcloning one or more identified pathways for industrial and/or natural product production purposes.