Patent ID: 11939624
Assignee: ROCHE SEQUENCING SOLUTIONS, INC.
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 11:
12. The method of claim 1, wherein the split-pool barcoding process comprises:
(i) splitting the cells or cell organelles into a plurality of first reaction volumes, wherein at least some of the plurality of first reaction volumes comprise multiple cells or multiple cell organelles;
(ii) appending assayable oligonucleotide subunits onto the ligation products in the plurality of first reaction volumes, wherein different first reaction volumes receive different assayable oligonucleotide subunits; and
(iii) pooling the contents of the plurality of first multiple reaction volumes to produce a first pool,
wherein the assayable oligonucleotide subunits added to the nucleic acids in (ii) become part or all of the cell origination barcode.