Patent ID: 11859232
Assignee: YISSUM RESEARCH DEVELOPMENT COMPANY OF THE HEBREW UNIVERSITY OF JERUSALEM LTD.
Field: Pharmaceuticals (Chemistry)
Classification: CPC C  A | IPC A  C

Claim 0:
1. A method of selecting an agent for treating a disease in a subject, the method comprising:
(a) exposing a plurality of isolated haploid human embryonic stem (ES) cells capable of differentiating into a terminally differentiated haploid cell selected from a haploid mature neuron, haploid mature pancreatic cell and haploid cardiomyocyte or haploid cells differentiated from said isolated haploid human ES cells, to an agent, wherein at least a portion of said plurality of cells comprises a distinct artificially inactivated or overactivated gene;
(b) selecting a cell of said plurality of cells which shows resistance to said agent;
(c) identifying in said cell said distinct artificially inactivated or overactivated gene; and
(d) analyzing the sequence and/or expression of said distinct artificially inactivated gene or activated gene in a cell sample of the subject, wherein an alteration in the sequence and/or level of expression of said distinct gene as compared to the sequence and/or expression of said distinct gene in a control sample is indicative of the agent to be used or ruled out for treating the disease in the subject.