Patent ID: 11933771
Assignee: SHIMADZU CORPORATION
Field: Measurement (Instruments)
Classification: CPC G | IPC G

Claim 3:
4. An analysis execution method performed by a liquid chromatograph that includes an analysis column, a junction and a fluorescence detector, including:
supplying a mobile phase to the fluorescence detector through the analysis column and the junction during determination of an analysis condition;
supplying a fluorescent reaction liquid to the fluorescence detector through the junction during the determination of the analysis condition;
introducing a sample into the mobile phase at a position farther upstream than the analysis column during the determination of the analysis condition;
generating a chromatogram based on an output signal of the fluorescence detector during the determination of the analysis condition;
determining a period from a point in time at which supply of the fluorescent reaction liquid starts to a point in time at which the supply of the fluorescent reaction liquid ends as a supply period based on the generated chromatogram by a supply period determiner during the determination of the analysis condition;
supplying the mobile phase to the fluorescence detector through the analysis column and the junction during an analysis of a sample;
supplying the fluorescent reaction liquid to the fluorescence detector through the junction in the supply period determined by the supply period determiner during the analysis of the sample;
introducing the sample into the mobile phase at a position farther upstream than the analysis column during the analysis of the sample; and
generating the chromatogram based on the output signal of the fluorescence detector during the analysis of the sample, wherein
the determining by the supply period determiner includes determining a start point in time of the supply period such that the fluorescent reaction liquid arrives at the junction later than a point in time at which supply of the mobile phase starts and before the sample introduced into the mobile phase arrives at the junction, and determining a point in time after the sample passes through the junction as an end point in time of the supply period, and
the supplying the fluorescent reaction liquid to the fluorescence detector includes starting supply of the fluorescent reaction liquid at the start point in time of the determined supply period and stopping the supply of the fluorescent reaction liquid at the end point in time of the supply period during the analysis of the sample.