Patent ID: 11913072
Assignee: ROCHE MOLECULAR SYSTEMS, INC.
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 2:
3. A method of detecting a MET exon 14 deletion comprising:
(a) obtaining a plasma or formalin fixed paraffin embedded tissue (FFPET) sample comprising RNA from an individual;
(b) carrying out a reverse transcription reaction on the RNA to produce cDNA;
(c) carrying out an amplification reaction comprising contacting the cDNA with (i) a primer set and a first probe labeled with a first label that specifically amplify and detect MET exon 13-14 junction, (ii) a primer set and a second probe labeled with a second label that specifically amplify and detect MET exon 14-15 junction, and (iii) a primer set and a third probe labeled with a third label that specifically amplify and detect MET exon 13-15 junction; and
(d) detecting the presence of a MET exon 14 deletion if an amplification product is formed and detected by the primer set and the third probe of (iii),
wherein steps (b) and (c) are carried out in the same vessels, wherein the first probe is specific for the junction point of the MET exon 13-14 junction, the second probe is specific for the junction point of the MET exon 14-15 junction, and the third probe is specific for the junction point of the MET exon 13-15 junction, and wherein the third probe comprises a sequence selected from the group consisting of SEQ ID NOs: 53-55.