Patent ID: 11860170
Assignee: PROCISEDX INC.
Field: Measurement (Instruments)
Classification: CPC G | IPC G

Claim 14:
15. The method according to claim 14, comprising:
contacting the sample with an additional antibody, which binds a first epitope of the additional biomarker, wherein the additional antibody is labeled with a third donor fluorophore;
contacting the sample with a further antibody, which binds a second epitope of the additional biomarker, wherein the further antibody is labeled with a third acceptor fluorophore;
incubating the sample for a time sufficient to obtain dual antibody labeled additional biomarker; and
exciting the sample having dual labeled additional biomarker using a light source to detect two fluorescence emission signals associated with fluorescence resonance energy transfer (FRET),
wherein the first, second, and third acceptor fluorophores are different.