Patent ID: 11873471
Assignee: ZHEJIANG UNIVERSITY
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 7:
8. A culture dish combination for embryo thawing and embryo transfer, comprising: a first culture dish (1), a connecting piece (3), and a second culture dish (2); the connecting piece (3) is connected between the first culture dish (1) and the second culture dish (2), and the first culture dish (1) is removable from the second culture dish (2) by tearing the connecting piece (3);
wherein the first culture dish (1) comprises a convex portion (12), a first annular wall (13), and a first dish bottom (14); the convex portion (12) is arranged on a side of the first annular wall (13) facing away from the second culture (2) and protrudes outward along a direction perpendicular to an axial direction of the first annular wall (13), and the first dish bottom (14) is surrounded by a bottom of the first annular wall (13) to define a first dish cavity (10) for thawing and laser-assisted hatching of a frozen embryo; and
wherein the second culture dish (2) comprises a third annular wall (23), a second annular wall (24), an annular stepped portion (25), and a second dish bottom (26); the third annular wall (23), the second annular wall (24), and the annular stepped portion (25) are sequentially arranged on the second dish bottom (26) from outside to inside in that order; the second dish bottom (26) is surrounded by a bottom of the third annular wall (23) to define a third dish cavity (22) between the third annular wall (23) and the second annular wall (24) for maintaining humidity of the second dish cavity and washing residual oil droplets before the embryo transfer by adding embryo culture medium, and the second dish bottom (26) is surrounded by a bottom of the second annular wall (24) to define a second dish cavity (20) for balancing culture medium before the embryo transfer;
wherein the annular stepped portion (25) comprises a fourth annular wall and a fifth annular wall disposed on the second dish bottom (26), the fourth annular wall is directly connected between an inner wall of the second annular wall (24) facing away from the third annular wall (23) and the fifth annular wall, the second dish bottom (26) is surrounded by the fifth annular wall to define a central dish cavity (21) in the second dish cavity (20) for embryo aggregation.