Patent ID: 11884966
Assignee: GRAIL, INC.
Field: Biotechnology (Chemistry)
Classification: CPC C  G | IPC C

Claim 0:
1. A method of detecting and treating a metastasis in a subject, the method comprising selecting a subject having a metastasis of a cancer with a tissue of origin, and administering a treatment to the subject effective to treat the metastasis, wherein:
(a) the selecting comprises (i) receiving results of an assay of a first biological sample of the subject measuring an absolute amount of cell-free DNA comprising a target sequence having a first methylation status (ii) identifying the measured absolute amount as being above a reference amount, and (iii) identifying a first non-cancer tissue as a location of the metastasis;
(b) the first biological sample comprises cell-free DNA molecules from the cancer, the first non-cancer tissue, and one or more second non-cancer tissues;
(c) the tissue of origin, the first non-cancer tissue, and the one or more second non-cancer tissues are different;
(d) cell-free DNA molecules from the first non-cancer tissue comprise the first methylation status of the target sequence;
(e) the first methylation status is distinct from one or more second methylation statuses of the target sequence in cell-free DNA molecules from the cancer and from the one or more second non-cancer tissues;
(f) the reference amount is an absolute amount of cell-free DNA comprising the target sequence having the first methylation status for one or more subjects without metastasis in the first non-cancer tissue; and
(g) the treatment is selected from the group consisting of chemotherapy, radiation therapy, surgical removal of tumor tissues, immunotherapy, hormone therapy, and stem cell therapy.