Patent ID: 11913046
Assignee: INBIOSE N.V.
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 6:
7. A genetically engineered yeast cell that produces 2′ fucosyllactose, wherein the yeast cell comprises:
a) at least one heterologous nucleic acid molecule encoding a transporter protein that facilitates export of 2′ fucosyllactose from the yeast cell;
b) at least one heterologous nucleic acid molecule encoding a guanosine diphosphate (GDP)-mannose-4,6-dehydratase (EC 4.2.1.47);
c) at least one heterologous nucleic acid molecule encoding a GDP-4-keto-6-D-deoxymannose epimerase-reductase (EC 1.1.1.271);
d) at least one heterologous nucleic acid molecule encoding a 2-α-L-fucosyltransferase (EC 2.4.1.69); and
e) at least one nucleic acid molecule encoding a lactose transporter having an amino acid sequence having 90% identity to peptide selected from the group consisting of SEQ ID NO: 24, 25, 26, 27 and 28.