Patent ID: 11932848
Assignee: UNIVERSITY OF MASSACHUSETTS
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 0:
1. A method for mapping genomic interaction frequencies, comprising:
a) contacting a first cell with a test compound; and
b) generating a carbon copy chromosome capture (5C) library from said first cell using the method comprising:
i) providing a chromosome conformation capture (3C) library constructed from cross-linked interacting chromatin segments derived from at least one cell, wherein said 3C library comprises a plurality of ligation products representative of interacting genomic loci, wherein each of said plurality of ligation products is formed by two adjacent restriction fragments;
ii) annealing unique primer pairs to at least a part of said plurality of ligation products; and
iii) ligating said annealed unique primer pairs together, thereby providing a carbon copy chromosome capture (5C) library, and

c) comparing interaction frequencies in said carbon copy chromosome capture (5C) library with interaction frequencies in a second carbon copy chromosome capture (5C) library generated from a second cell not exposed to said test compound; and
d) creating a genomic interaction map based upon said compared interaction frequencies.