Patent ID: 11970718
Assignee: CARMINE THERAPEUTICS PTE. LTD.
Field: Pharmaceuticals (Chemistry)
Classification: CPC C  A  B | IPC A  B  C

Claim 0:
1. A preparation comprising a population of red blood cell extracellular vesicles (RBCEVs) loaded with a DNA cargo and a cationic reagent, wherein:
the RBCEVs have a size between 50 nm and 1,000 nm in diameter,
the DNA cargo has a length between 2,000 bases and 40,000 bases;
the cationic reagent complexes with the DNA cargo and is a polyethylenimine (PEI) reagent
and further wherein the preparation is characterized in that:
when the preparation is contacted with recipient mammalian cells, it is more effective at transfecting the mammalian cells with the DNA cargo than is an otherwise comparable reference preparation loaded by electroporation.