Patent ID: 11913082
Assignee: LABORATORY CORPORATION OF AMERICA HOLDINGS
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 0:
1. A method for simultaneously determining the viral load and genotype of a respiratory syncytial virus (RSV) in a biological sample, comprising:
a) amplifying a nucleic acid sequence encoding an RSV open reading frame (ORF) in a biological sample using a single set of primers that specifically amplifies an RSV A ORF and/or an RSV B ORF, wherein said single set of primers is a single primer pair, and at least two nucleic acid probes in a quantitative polymerase chain reaction assay, wherein at least one probe specifically binds to an RSV subtype A nucleic acid sequence and at least one probe specifically binds to an RSV subtype B nucleic acid sequence, and wherein the at least two nucleic acid probes are differentially labeled;
b) amplifying an RSV F ORF in the biological sample with a second single set of primers;
c) sequencing the RSV F amplification product; and
d) determining the viral load based on the amount of the amplified nucleic acid sequence produced by the quantitative PCR assay and determining the RSV subtype(s) present in the biological sample based on the binding of at least one probe in step (a), on the sequence determined in step (c), or both.