Patent ID: 11913942
Assignee: IDEXX LABORATORIES, INC.
Field: Measurement (Instruments)
Classification: CPC G  C | IPC C  G

Claim 10:
11. The method of claim 7, further comprising
(a) conducting a calibrator first reaction sequence comprising:
i. forming calibrator first reaction mixtures by individually contacting each calibrator of a set of calibrators comprising known amounts of the analyte with an anti-analyte antibody, a conjugate comprising the analyte and an enzyme, and a substrate that produces a signal when in contact with the enzyme, and
ii. measuring signal from each of the calibrator first reaction mixtures;
iii. normalizing a reaction rate from each of the calibrator first reaction mixtures by accounting for background associated with each of the calibrator first reaction mixtures,

(b) conducing a calibrator second reaction sequence comprising,
i. forming calibrator second reaction mixtures by contacting each calibrator of the set of calibrators with the substrate,
ii. measuring signal from the calibrator second reaction mixtures;
iii. normalizing a reaction rate for each calibrator in the calibrator second reaction mixtures by accounting for background associated with the calibrator second reaction mixtures,

(c) subtracting the normalized rates of step (b)(iii) from the normalized rates of step (a)(iii) to provide a final reaction rate for each of the calibrators,
(d) generating a normalized standard curve based upon the final reaction rate for each of the calibrators,
(e) determining the amount of the analyte in the sample by comparing the normalized reaction rate for the sample to the normalized standard curve.