Patent ID: 11952518
Assignee: BEIJING FLUORESCENCE BIOTECHNOLOGY CO. LTD
Field: Measurement (Instruments)
Classification: CPC C  G  Y | IPC C  G

Claim 1:
2. A method for preparing the fluorescent dye with phenanthridine and benzothiazole conjugated according to claim 1, comprising the following steps:
S1, reacting a compound of formula 1 with methyl iodide for 4 h at 210° C. under the protection of nitrogen to prepare a compound of formula 3;, , S2, dissolving a compound of formula 4 and 6-bromo-1-hexanoic acid into absolute ethyl alcohol under the protection of nitrogen, heating to 35° C. under the protection of nitrogen, and reacting for 4 h so as to prepare a compound of formula 6;, , S3, dissolving the compound of formula 3 and the compound of formula 6 into ethyl alcohol, adding triethylamine under the protection of nitrogen, and reacting for 3 h to prepare a compound of formula 7;, , S4, dissolving the compound of formula 7 and 2-succinylimido-1,1,3,3-tetramethylurea tetrafluoroborate into acetonitrile, adding triethylamine, and reacting for 4 h to prepare an acetonitrile solution of a compound of formula 8;, , S5, dissolving a compound of formula 9 into dimethyl formamide (DMF), adding 2-succinylimido-1,1,3,3-tetramethylurea tetrafluoroborate, stirring for 30 min, then adding triethanolamine, reacting for 30 min at 20-25° C., adding ethanediamine, and reacting for 12-16 h to prepare a compound of formula 11; and, , S6, dissolving the compound of formula 11 into DMF, adding the acetonitrile solution of the compound of formula 8, and reacting for 12-16 h to obtain the fluorescent dye with phenanthridine and benzothiazole conjugated.