Patent ID: 11859219
Assignee: FLAGSHIP PIONEERING INNOVATIONS V, INC.
Field: Biotechnology (Chemistry)
Classification: CPC C  A | IPC A  C

Claim 6:
7. The method of claim 1, wherein
the preassembled ribonucleoprotein composition further comprises a chemical agent or a physical agent or a combination of both chemical and physical agents, or
the method further comprises a step of treating the population of protoplasts with a chemical agent or a physical agent or a combination of both chemical and physical agents;
wherein the chemical agent is at least one selected from the group consisting of solvents, fluorocarbons, glycols or polyols, surfactants; primary, secondary, or tertiary amines and quaternary ammonium salts; organosilicone surfactants; lipids, lipoproteins, lipopolysaccharides; acids, bases, caustic agents; peptides, proteins, or enzymes; cell-penetrating peptides; RNase inhibitors; cationic branched or linear polymers; dendrimers; counter-ions, amines or polyamines, osmolytes, buffers, and salts; polynucleotides; transfection agents; antibiotics; non-specific DNA double-strand-break-inducing agents; chelating agents; and antioxidants; and
wherein the physical agent is at least one selected from the group consisting of particles or nanoparticles, magnetic particles or nanoparticles, abrasive or scarifying agents, needles or microneedles, matrices, and grids.