Patent ID: 11965210
Assignee: ROCHE SEQUENCING SOLUTIONS, INC.
Field: Measurement (Instruments)
Classification: CPC C  G | IPC C  G

Claim 0:
1. A system for identifying a nucleotide or nucleotide analog or portion thereof, the system comprising:
a chip comprising at least one cell, the cell comprising an electrode pair and a nanopore disposed in a membrane, wherein the electrode pair is associated with a variable voltage source and configured to apply a variable voltage across the nanopore of the membrane;
a memory device comprising code instructions, and
a processor communicatively coupled to the memory device, wherein the processor is configured to execute the code instructions that are stored in the memory device to cause the system to:
vary the voltage applied across the nanopore when a nucleotide or nucleotide analog or portion thereof is inserted into the nanopore, wherein the voltage is varied across a range of voltages from 120 mV to 150 mV;
measure an electrical signal at a plurality of applied voltages with at least one of the electrode pair while the nucleotide or nucleotide analog or portion thereof is inserted within the nanopore; and
identify the nucleotide or nucleotide analog or portion thereof that is inserted within the nanopore based on the measured electrical signal at the plurality of applied voltages.