Patent ID: 11954856
Assignee: NANTOMICS, LLC
Field: Computer technology (Electrical engineering)
Classification: CPC G | IPC G

Claim 0:
1. A cell counting computing apparatus comprising:
at least one computer-readable memory storing cell counting software instructions;
at least one processor coupled with the at least one computer-readable memory and that operates as a cell counting engine upon execution of the cell counting software instructions to:
obtain, in the at least one computer-readable memory, a whole image comprising images of cells;
segment the whole image into image areas based on a first global thresholding filter with respect to a first threshold;
select a plurality of patches associated with the image areas;
generate an intensity image based on intensity vectors for each patch in the plurality of patches by determining an optical density of each patch;
generate at least one cell mask with at least one cell seed by iteratively segmenting the intensity image based on a second global thresholding filter with respect to a second iteratively reducing threshold;
generate a distance map from the at least one cell mask via a chamfer distance transform;
distinguish cell segments within the plurality of patches via a watershed transform of the distance map and at least one cell seed; and
configure a device to display a cell count for at least one patch in the plurality of patches based on counting the cell segments.