Patent ID: 11863867
Assignee: LEICA BIOSYSTEMS IMAGING, INC.
Field: Audio-visual technology (Electrical engineering)
Classification: CPC G  H | IPC G  H

Claim 16:
17. A method of acquiring an image, the method comprising:
acquiring image stripes of at least a portion of a sample on a glass slide by acquiring frames representing the image stripe using both an imaging line-scan camera and a tilted focusing line-scan camera;
adding focus points to a focus map representing positions of best focus of acquired frames to a focus map while acquiring the image stripe, wherein the image stripes are acquired by acquiring a reference stripe from a location identified by a tissue-finding algorithm, the location identified by the tissue-finding algorithm having the most tissue among a plurality of other locations, acquiring image stripes in sequence from a first side of the reference stripe to a first edge of a scan area of the sample, and acquiring image stripes in sequence from a second side of the reference stripe, which is opposite the first side of the reference stripe, to a second edge of the scan area, which is opposite the first edge of the scan area, the acquired image stripes extending along a scan axis and offset relative to each other image stripe along a stripe acquisition axis separate from the scan axis;
determining whether or not to re-acquire one or more of the image stripes based on the focus map; and
assembling the acquired image stripes into a composite image.