Patent ID: 11866765
Assignee: GENINUS INC.
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 0:
1. A method for preparing a DNA library for nucleic acid sequencing, the method comprising:
ligating an adapter comprising a barcode sequence to both ends of a DNA fragment extracted and fragmented from a target sample;
separating the ligated DNA fragment into single strands; and
amplifying the single-stranded DNA fragment ligated with the adapter through a polymerase chain reaction by using a primer that recognizes the adapter,
wherein the barcode sequence has a length of 2 nt to 10 nt and is arranged to include the position of a first nucleotide in the adapter, on the basis of a terminal region of the adapter ligated with the DNA fragment, and
wherein the second nucleotide in the adaptor is either G or C.