Patent ID: 11963981
Assignee: AUTOLUS LIMITED
Field: Pharmaceuticals (Chemistry)
Classification: CPC C  A | IPC A  C

Claim 7:
8. A nucleic acid construct according to claim 5, wherein the anti-CD22 CAR is in an ScFv format, the nucleic acid construct having the general structure:
AgBD1-spacer1-TM1-endo1-coexpr-AgBD2-spacer2-TM2-endo2 or
AgBD2-spacer2-TM2-endo2-coexpr-AgBD1-spacer1-TM1-endo1,, wherein:
AgBD1 is a nucleic acid sequence encoding an antigen binding domain of the first CAR,
Spacer1 is a nucleic acid sequence encoding a spacer of the first CAR,
TM1 is a a nucleic acid sequence encoding a transmembrane domain of the first CAR,
Endo1 is a nucleic acid sequence encoding an endodomain of the first CAR,
Coexpr is a nucleic acid sequence enabling co-expression of the first and second CARs,
AgBD2 is a nucleic acid sequence encoding an antigen binding domain of the second CAR,
Spacer2 is a nucleic acid sequence encoding a spacer of the second CAR,
TM2 is a a nucleic acid sequence encoding a transmembrane domain of the second CAR, and
Endo2 is a nucleic acid sequence encoding an endodomain of the second CAR.