Patent ID: 11899016
Assignee: VENTANA MEDICAL SYSTEMS, INC.
Field: Measurement (Instruments)
Classification: CPC G  A  C | IPC C  G

Claim 0:
1. An immunohistochemical method of identifying tumor infiltrating PD-L1 positive immune cells in a PD-L1 positive tumor via brightfield microscopy, said method comprising:
contacting a tissue sample with a first primary antibody specific to PD-L1 protein in a manner to effect binding of the first primary antibody to the tissue sample in proximity to PD-L1 protein expressed by the tissue sample;
depositing a first enzyme in proximity to the first primary antibody bound to the tissue sample;
reacting the first enzyme with a first substrate to effect deposition of a first brightfield microscopy-detectable dye on the tissue sample in proximity to the first primary antibody;
contacting the tissue sample with a second primary antibody specific to at least one immune cell marker protein in a manner to effect binding of the second primary antibody to the tissue sample in proximity to immune cell marker protein expressed by the tissue sample;
depositing a second enzyme in proximity to the second primary antibody bound to the tissue sample and
reacting the second enzyme with a second substrate to effect deposition of a second brightfield microscopy-detectable dye on the tissue sample in proximity to the second primary antibody,
wherein (i) the first brightfield microscopy-detectable dye is 4-(dimethylamino) azobenzene-4′-sulfonamide and the second brightfield microscopy-detectable dye is tetramethylrhodamine, or (ii) the first brightfield microscopy-detectable dye is tetramethylrhodamine and the second brightfield microscopy-detectable dye is 4-(dimethylamino) azobenzene-4′-sulfonamide.