Patent ID: 11905550
Assignee: SERVICE DEPARTMENTAL METROPOLITAIN D'INCENDIE ET DE SECOURS (SDMIS)
Field: Measurement (Instruments)
Classification: CPC C  G  B | IPC B  C  G

Claim 0:
1. A process for preparing a biological sample comprising biological species, implemented in a preparation system,
wherein the preparation system comprises a device comprising:
a housing comprising at least one opening,
a first channel provided in the housing,
a second channel provided in the housing,
a chamber into which the first channel and the second channel lead, wherein the chamber comprises a rough bearing surface arranged on the bottom of the chamber which extends over a majority of the bottom of the chamber,
a filter separating the chamber into two distinct spaces, wherein the first injection channel leads to a first space and the second channel leads to a second space, wherein the first space and the second space are partially superimposed such that the first space is a lower space in the chamber and is located under the filter and the second space is an upper space in the chamber and is located above the filter, such that the first space and the second space are in contact with each other in an superimposed area via the filter separating the first space and the and second space, wherein the filter has a porosity suitable for retaining the biological material to be analyzed,
the process comprising:
injecting the biological sample in the form of a fluid via the first channel to concentrate biological species in the first space of the chamber of the device,
conducting mechanical lysis of the biological species in the chamber by grinding the biological species by abrasion on the rough bearing surface, and separating cell membranes from an intracellular material,
eluting the intracellular material by injecting an amplification buffer through the first channel of the device, to elute the material via the second channel,
injecting an immunological buffer fluid via the second channel of the device and at least partially eluting the biological species with the immunological buffer fluid.