Patent ID: 11965171
Assignee: BASF SE
Field: Biotechnology (Chemistry)
Classification: CPC C  A | IPC A  C

Claim 3:
4. A method for controlling weeds at a locus for growth of a plant, the method comprising:
(a) applying an herbicide composition comprising CESA-inhibiting herbicides to the locus; and (b) planting a seed at the locus, wherein the seed is capable of producing a plant that comprises in at least some of its cells a polynucleotide operably linked to a promoter operable in plant cells, the promoter capable of expressing a mutated CESA polypeptide encoded by the polynucleotide, the expression of the mutated CESA polypeptide conferring to the plant tolerance to CESA-inhibiting herbicides, wherein the mutated CESA polypeptide comprises one or more of the following motifs:
i) Motif 1a:, (SEQ ID NO: 78)

[V/I][A/V]G[V/I/F][S/T][Y/D/N/A]A[V/I/L][N/S/G][S/

 

N]G[Y/F/E][Q/D/G/E/H][S/A]WG[P/A]L[F/M/L]G[K/R][L/

 

V][F/L]F,

wherein the amino acid at position 5 within said motif is substituted by any other amino acid than S or T;
wherein the amino acid at position 16 within said motif is substituted by any other amino acid; and/or
wherein the amino acid at position 17 within said motif is substituted by any other amino acid than P or A;
ii) Motif 2a:, (SEQ ID NO: 81)

[V/L/I]W[S/A][V/A/I]LL[A/S]S[I/F/V][F/L][S/T][L/V]

 

[L/M/V/1]WV[R/K][I/V][N/D]PF,

 wherein the amino acid at position 8 within said motif is substituted by any other amino acid, and/or position 11 by any other amino acid than S or T;
wherein the mutated CESA polypeptide comprises an amino acid sequence having, over the full-length, at least 80% sequence identity to SEQ ID NO: 28, and wherein the amino acid sequence of the mutated CESA polypeptide differs from the wildtype amino acid sequence at one or more positions corresponding to positions 1009, 1010, 1013, 1052, and 1055 of SEQ ID NO: 1.