Patent ID: 11890291
Assignee: INNOVA AGRI BIO PARK PRIVATE LIMITED
Field: Pharmaceuticals (Chemistry)
Classification: CPC A  B | IPC A

Claim 16:
17. A method for preparation of encapsulated Vitamin D PLGA nanoparticles, comprising:
a. preparing a reaction mixture of PLGA with Vitamin D dissolved in ethyl acetates solution;
b. dropwise addition of Pluronics to the reaction mixture of step (a) followed by sonication of the emulsion under ice bath;
c. solubilizing the emulsion by magnetic stirring at 1000 rpm for 2.5 hours at 25° C.;
d. filtration of emulsion;
e. cooling of the emulsion at 4° C. during overnight at final concentration of 10 mg/mL;
f. centrifugation at 15000 rpm for 30 minutes and resuspension in ultrapure water.