Patent ID: 11952621
Assignee: CEPHEID
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 8:
9. The primer set of claim 1, wherein the second primer comprises oligonucleotides in the form of, or capable of forming, at least two second primers capable of hybridizing to the second template strand, wherein the at least two second primers comprise a second outer primer and a second inner primer,
the second outer primer comprising a primer sequence e that specifically hybridizes to second template strand sequence e′; and
the second inner primer comprising a single-stranded primer sequence f that specifically hybridizes to second template strand sequence f′, wherein f′ is adjacent to, and 5′ of, e′, and wherein single-stranded primer sequence f is linked at its 5′ end to a first strand of a double-stranded primer sequence comprising:
a primer sequence e adjacent to, and 5′ of, single-stranded primer sequence f; and
a clamp sequence g adjacent to, and 5- of, primer sequence e, wherein clamp sequence g is not complementary to second strand template sequence h′, which is adjacent to, and 3′, of second strand template sequence e′.