Patent ID: 11926833
Assignee: LIVING CARBON PBC
Field: Biotechnology (Chemistry)
Classification: CPC C  Y | IPC C

Claim 23:
24. A polynucleotide construct comprising:
an RNAi polynucleotide comprising a polynucleotide sequence that targets an endogenous glycolate transporter Plgg1 present in a tree plant cell, wherein the RNAi polynucleotide comprises a sense strand comprising at least 21 contiguous nucleotides of the endogenous glycolate transporter Plgg1 and an antisense strand that is fully complementary to the at least 21 contiguous nucleotides of the sense strand, wherein the sense strand and the antisense strand form a dsRNA, wherein when expressed in the tree plant cell, the RNAi polynucleotide reduces expression of the endogenous glycolate transporter Plgg1 by about 20% to 80% of expression of endogenous glycolate transporter Plgg1 in a tree plant cell where the RNAi polynucleotide is not present, and wherein the tree plant cell is from a tree selected from Populus, Pinus taeda, and Picea abies.