Patent ID: 11866725
Assignee: NOVARTIS AG
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 21:
22. A lentiviral transfer vector comprising, from 5′ to 3′, one or more of the following elements in operable association:
(a) a promoter to drive the expression of viral genes,
(b) a packaging signal (psi) comprising a major splice donor site (SD),
(c) a partial gag sequence consisting of 150-250 nucleotides,
(d) a partial env sequence consisting of a sequence having at least 95% sequence identity to SEQ ID NO: 60,
(e) a Rev-response element (RRE),
(f) a partial env sequence comprising splice acceptor site (SA7) and consisting of a sequence having at least 95% sequence identity to SEQ ID NO: 64,
(g) a central polypurine tract (cPPT) comprising a splice acceptor site (SA1),
(h) a subgenomic promoter,
(i) a gene encoding EGFP and/or a heterologous nucleic acid sequence, and
(j) a post-transcriptional regulatory element, which optionally comprises a woodchuck hepatitis virus PRE (WPRE) or a hepatitis B virus isolate bba6 PRE (HPRE).