Patent ID: 11857965
Assignee: HEWLETT-PACKARD DEVELOPMENT COMPANY, L.P.
Field: Chemical engineering (Chemistry)
Classification: CPC B  F | IPC B  F

Claim 14:
15. The method of claim 14, wherein flowing the fluid through the fluid flow microfluidic channel comprises flowing the fluid in a first direction, and wherein the method further comprises actuating a second microfluidic valve, which is a normally-closed valve, to allow the fluid to flow in a second direction, wherein the second microfluidic valve comprises a second blister formed in the flexible blister layer, wherein the substrate further comprises a second microfluidic channel formed in the substrate, wherein the second microfluidic channel is in fluid communication with the second blister, wherein the sealing layer separates the second blister from fluid communication with the fluid flow microfluidic channel, and wherein puncturing the sealing layer places the second blister into fluid communication with the fluid flow microfluidic channel to allow fluid to flow between the fluid flow microfluidic channel and the second microfluidic channel through the second blister volume.