Patent ID: 11940442
Assignee: AGILENT TECHNOLOGIES, INC.
Field: Measurement (Instruments)
Classification: CPC G | IPC G

Claim 0:
1. A method of determining a single metabolic potential of a cell sample that is representative of both mitochondrial respiration and glycolysis, the method comprising the steps of:
measuring an initial oxygen consumption rate and an initial extracellular acidification rate of the cell sample;
thereafter, simultaneously administering to the cell sample a mitochondrial uncoupling agent and an ATP synthase inhibitor;
thereafter, simultaneously measuring a subsequent oxygen consumption rate and a subsequent extracellular acidification rate of the cell sample; and
determining a single metabolic potential of the cell sample that is representative of both mitochondrial respiration and glycolysis, wherein the single metabolic potential is plotted graphically where the Y axis represents oxygen consumption rate and the X axis represents extracellular acidification rate.