Patent ID: 11884957
Assignee: GEN-PROBE INCORPORATED
Field: Measurement (Instruments)
Classification: CPC C  G | IPC C  G

Claim 4:
5. The method of claim 4, wherein detecting the Eggerthella, Prevotella, and Lactobacillus cleavage products comprises
contacting the Eggerthella cleavage product with a first FRET cassette comprising a first fluorescent label and a first quencher, contacting the Prevotella cleavage product with a second FRET cassette comprising a second fluorescent label and a second quencher, and contacting the Lactobacillus cleavage product with a third FRET cassette comprising a third fluorescent label and a third quencher, optionally wherein the first, second, and third quenchers are the same,
wherein each FRET cassette hybridizes with the respective cleavage product so as to form a second Eggerthella, Prevotella, or Lactobacillus cleavage structure capable of being cleaved by the flap endonuclease,
wherein if the Eggerthella cleavage product is present, the first fluorescent label is released from the first FRET cassette comprising the first quencher,
wherein if the Prevotella cleavage product is present, the second fluorescent label is released from the second FRET cassette comprising the second quencher, and
wherein if the Lactobacillus cleavage product is present, the third fluorescent label is released from the third FRET cassette comprising the third quencher; and
detecting the released first, second, or third fluorescent label.