Patent ID: 11912745
Assignee: UNIVERSITY OF MARYLAND, COLLEGE PARK
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 0:
1. A fusion protein comprising:
i) five to ten tandemly connected intercellular adhesion molecule-1 (ICAM-1) targeting segments, wherein each ICAM-1 targeting segment comprises SEQ ID NO: 27 (NNQKLVNIKEKVAQIEA);
ii) an enzyme segment that can be catalytically active at the pH of a lysosome, wherein the enzyme segment comprises Acid sphingomyelinase (ASM), Alpha galactosidase, or Glucocerebrosidase;
iii) a first protease cleavage sequence segment between i) and ii), and optionally, one or more of:
iv) a secretion signal;
v) a protein purification tag; and
vi) a second protease cleavage sequence.