Patent ID: 11937586
Assignee: OZGENE HOLDINGS PTY LTD
Field: Biotechnology (Chemistry)
Classification: CPC A  C | IPC A  C

Claim 9:
10. A method of producing a chimeric male mouse, the method comprising (1) implanting a male mouse embryo produced according to the method of claim 1 into a cognate pseudopregnant mouse, wherein the male mouse embryo comprises germ cells having the disrupted GILZ gene therein, wherein the male mouse embryo further comprises a donor pluripotent cell that comprises (a) the GILZ gene lacking a disruption, and (b) a genetic modification in its genome; and (2) gestating the male mouse embryo of (1) under conditions suitable for development of the embryo, thereby generating a chimeric male mouse with the disrupted GILZ gene and the genetic modification in its germ line.