Patent ID: 11932859
Assignee: REGENERON PHARMACEUTICALS, INC.
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 1:
2. The method of claim 1, wherein the assembling in step (c) comprises:
(i) annealing the first complementary sequence of the first joiner oligo to the first digested nucleic acid, annealing the second complementary sequence of the first joiner oligo to the second nucleic acid, annealing the first complementary sequence of the second joiner oligo to the second nucleic acid, annealing the second complementary sequence of the second joiner oligo to the third nucleic acid, annealing the first complementary sequence of the third joiner oligo to the third nucleic acid, and annealing the second complementary sequence of the third joiner oligo to the first digested nucleic acid; and
(ii) ligating the first digested nucleic acid to the first joiner oligo, ligating the first joiner oligo to the second nucleic acid, ligating the second nucleic acid to the second joiner oligo, ligating the second joiner oligo to the third nucleic acid, ligating the third nucleic acid to the third joiner oligo, and ligating the third joiner oligo to the first digested nucleic acid.