Patent ID: 11913065
Assignee: GUARDENT HEALTH, INC.
Field: Biotechnology (Chemistry)
Classification: CPC C  G | IPC C  G

Claim 0:
1. A method for sequencing polynucleotides derived from a population of cell-free nucleic acids from a sample of a subject, the method comprising:
(a) ligating a plurality of the cell-free nucleic acids with molecular barcodes from a set of molecular barcodes to produce tagged parent polynucleotides,
wherein ligating comprises using more than a 10X molar excess of molecular barcodes as compared to the cell-free nucleic acids in the population, and
wherein at least 20% of the cell-free nucleic acids from the population are attached to molecular barcodes at both ends of a molecule of the cell-free nucleic acids;

(b) amplifying a plurality of the tagged parent polynucleotides to generate amplified progeny polynucleotides; and
(c) sequencing at least a subset of the amplified progeny polynucleotides to generate sequencing reads representing sequences of the amplified progeny polynucleotides, wherein a plurality of the sequencing reads comprises sequences of the molecular barcodes and of the cell-free nucleic acids.