Patent ID: 11883435
Assignee: INDIANA UNIVERSITY RESEARCH AND TECHNOLOGY CORPORATION
Field: Pharmaceuticals (Chemistry)
Classification: CPC A | IPC A

Claim 0:
1. A method for generating a population of hematopoietic stem cells capable of engraftment, comprising:
harvesting a portion of cells from bone marrow, umbilical cord blood, and/or peripheral blood of a subject in ambient air;
transferring the harvested cells directly into a chilled collection environment of about 1.5-5° C., wherein chilled phosphate buffered saline (PBS) with heparin is present in the chilled collection environment at the time of transfer or immediately after the transfer;
processing the harvested cells to generate a population of hematopoietic stem cells, wherein the harvested cells are maintained at temperatures of about 1.5-5° C. throughout processing.