Patent ID: 11891647
Assignee: NATIVE MICROBIALS, INC.
Field: Computer technology (Electrical engineering)
Classification: CPC C  G | IPC C  G

Claim 13:
14. A method of forming a bioreactive modificator based on microorganism strain absolute cell count analysis, the bioreactive modificator configured to alter a property in a target biological environment, comprising:
obtaining at least two samples sets, each set comprising a plurality of samples;
for an identified sample from a plurality of samples from a sample set, detecting the presence of a plurality of microorganism strains;
identifying, by sequencing DNA in each sample, a set of DNA representing whole genomes of the plurality of microorganism strains;
obtaining a set of sequencing reads associated with the plurality of microorganism strains from sequencing the DNA in the sample;
comparing each sequencing read from the set of sequencing reads with each DNA representing a whole genome in the set of DNA representing whole genomes to identify a match between a sequencing read and a DNA representing a whole genome;
determining, based on the comparing, a relative frequency of occurrence for each microorganism strain within the sample;
determining the absolute cell count of each microorganism strain in each sample from a total quantity of microorganisms and the relative frequency for each microorganism strain;
measuring RNA for each microorganism strain by sequencing cDNA in each sample and mapping the sequenced cDNA to the DNA representing a whole genome of each microorganism strain;
determining an activity for each microorganism strain in each sample based on the quantity of cDNA reads mapped to the DNA representing a whole genome of each microorganism strain;
generating a gene expression profile associated with each microorganism strain based on the RNA associated with each microorganism strain;
identifying active microorganism strains based on the activity for each microorganism strain;
identifying genetic similarities between at least two active microorganism strains based on the gene expression profile associated with each microorganism strain from the active microorganism strains to identify at least one functionality of interest;
selecting at least one compound associated with a metabolic pathway based on the identified at least one functionality of interest; and
combining the selected compound with a carrier medium to form a bioreactive modificator configured to alter a property associated with the at least one metadata of a target biological environment when the bioreactive modificator is introduced into that target biological environment.