Patent ID: 11878088
Assignee: THE UNIVERSITY OF MEMPHIS RESEARCH FOUNDATION
Field: Pharmaceuticals (Chemistry)
Classification: CPC A  C  D | IPC A  C  D

Claim 9:
10. A method of promoting or directing formation of a tissue in a subject at a target site comprising contacting the site with a barrier membrane comprising electrospun chitosan nanofibers produced according to a method comprising:
(a) electrospinning an acidic solution comprising chitosan to produce a chitosan nanofiber comprising immobilized salt residues;
(b) removing one or more immobilized salt residues on the electrospun chitosan nanofiber with an organic solvent, thereby generating one or more free amino groups on the chitosan nanofibers; and
(c) contacting at least one free amino group with an amino protecting reagent, wherein the tissue is bone tissue and the method prevents soft tissue migration into the target site.