Patent ID: 11892390
Assignee: NEW YORK UNIVERSITY
Field: Measurement (Instruments)
Classification: CPC G | IPC G

Claim 12:
13. A system for characterizing a parameter of a sample by holographic microscopy, comprising:
a holographic microscope including a laser beam source and an objective lens, the laser beam scattering from the sample and interacting with an unscattered portion of the laser beam to provide a holographic scattering pattern;
an image collection device for collecting image data characteristic of the scattering pattern from the holographic microscope; and
a computer system including a processor and memory, the memory having stored thereon computer readable instructions, the computer readable instructions configured to:
analyze the image data via computer software executed by the computer system, the computer software including a Lorenz-Mie methodology and executed by a graphical processing unit to provide substantially real time output of parameters characteristic of the sample;
receive holographic image data of the sample;
determine a first estimate of the number of objects in the holographic image data, each object associated with a set of concentric bright and dark rings;
determine, for each set of concentric bright and dark rings, by a voting algorithm, a centroid defined by an approximate x, y position in a plane, with each pixel of the image data voting for pixels in a transformed image that may be centroids;
determine an estimate of the axial position (z) of each of the objects;
determine by Lorenz-Mie analysis an estimate of each of the objects' radius and refractive index;
compare one of the radius and refractive index for each of the objects the same of radius or refractive index of a control uncoated sample; and
determine for each one of the objects if that one object is coated.