Patent ID: 11912746
Assignee: 2SEVENTY BIO, INC.
Field: Pharmaceuticals (Chemistry)
Classification: CPC C  A | IPC C

Claim 0:
1. A method of editing a human PD-1 gene in a cell comprising: introducing a polynucleotide encoding a polypeptide into the cell,
wherein the polypeptide comprises an I-OnuI homing endonuclease (HE) variant comprising an amino acid sequence at least 95% identical to the amino acid sequence set forth in SEQ ID NO: 61, and that binds and cleaves the target polynucleotide sequence in the human program cell death 1 (PD-1) gene set forth in SEQ ID NO: 30;
wherein expression of the polypeptide creates a double strand break at a target site in a human PD-1 gene; and
wherein the cell is a hematopoietic cell.