Patent ID: 11895960
Assignee: KWS SAAT SE & CO. KGAA
Field: Biotechnology (Chemistry)
Classification: CPC A  C  Y | IPC A  C

Claim 9:
10. A method of generating a plant according to claim 1, comprising the steps of:
i) subjecting seeds of a plant to a sufficient amount of the mutagen ethylmethane sulfonate to obtain M1 plants,
ii) allowing sufficient production of fertile M2 plants,
iii) isolating genomic DNA of M2 plants and
iv) selecting individuals comprising said nucleotide sequence comprising said one or more mutations of the loop1 consensus sequence, wherein said substitution confers the biological activity of a haploid inducer, and wherein the plant is homozygous with respect to the mutation and is not able to express a native CENH3 protein.