Patent ID: 11946087
Assignee: AMYRIS BIO PRODUCTS PORTUGAL, UNIPESSOAL, LDA
Field: Biotechnology (Chemistry)
Classification: CPC C  A | IPC A  C

Claim 39:
40. A method for co-production of two or more isoprenoids, the method comprising:
(a) culturing, in a culture medium, a host cell genetically modified with one or more heterologous nucleic acids encoding one or more enzymes in a first biosynthetic pathway to produce a first isoprenoid, and with one or more heterologous nucleic acids encoding one or more enzymes in a second biosynthetic pathway to produce a second isoprenoid, which has a molecular weight that is different from the first isoprenoid wherein the first isoprenoid is produced and secreted into the culture medium, and wherein the second isoprenoid is produced and remains associated with the host cell, wherein the first isoprenoid is a sesquiterpene and the second isoprenoid is one or more carotenoids; wherein the ratio of the one or more carotenoids to the sesquiterpene is between about 0.001 to 2% by weight or between about 0.1 to about 1% by weight;
(b) recovering the first isoprenoid from the culture medium; and
(c) recovering the second isoprenoid from the host cell.