Patent ID: 11891645
Assignee: NOVOZYMES A/S
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 26:
27. A protease variant, comprising at least one substitution corresponding to positions 2, 17, 22, 41, 41, 42, 45, 46, 54, 55, 57, 59, 60, 61, 62, 63, 64, 66, 69, 80, 82, 84, 87, 98, 99, 102, 103, 112, 114, 115, 129, 131, 134, 149, 159, 167, 169, 199, 200, 205, 207, 209, 211, 213, 217,242, 250, 266, 267, 269, 270, 271, 272, 274, 278, 279, 280, 284, 288, 291, 292, 294, 296, 301, 307, 314, 329, 331, 333, 336, 362, and 363 of the polypeptide of SEQ ID NO: 3, and the variant has protease activity and wherein the variant has at least 90%, but less than 100% sequence identity to the mature polypeptide of SEQ ID NO: 3, and wherein the substitution at one or more positions is selected from the group consisting of I2L, A17P, P22V, Q41L, Q41T, Q41V, Q41Y, F42R, K45S, A46L, A46W, Q54R, Q54S, Q54V, Q54Y, F55L, F55N, F55T, F55Y, K57H, K57L, K57V, I59D, I59K, I59R, I59S, I59V, S60I, S60L, S60Q, S60R, S60Y, S61A, S61F, S62H, S62I, S62L, S62R, S62V, T63A, T63E, T63G, T63R, T63V, T64C, T64D, T64M, S66G, T69A, T69F, T69P, T69R, T69Y, S80G, S80L, S80N, S80T, A82H, 184G, N87P, T98C, G99V, T102A, T102H, T103F, T103I, T103V, Q112H, Q112M, Q112R, Q112S, G114A, G114C, G114L, G114P, N115Y, E129L, E129S, E129V, E129Y, N131I, N131S, N131V, Q134A, Q134L, Q134R, A149D, Y159F, Y159L, T167A, T167L, T167S, T167W, 1169S, F199Y, M200L, A205S, Q207N, V209P, P211F, P211S, T213S, F217I, A242E, K250R, V266A, V266F, V266L, V266S, V266T, V266Y, D267F, D267L, D267T, D267V, Q269C, Q269F, Q269I, Q269S, Q269T, Q269V, Q269X, I270A, I270C, I270L, I270S, V271F, V271I, V271K, V271M, V271S, V271Y, S272A, S272N, G274R, G278S, V279I, D280Y, C284A, T288A, T288C, T288G, S291V, V292S, S294G, V296P, V296R, V296T, I301T, P307T, F314L, S329D, S329V, S331A, S331G, S331T, P333V, S336G, S336L, S336T, T362R, and A363T, and wherein the substitution at the one or more positions provides a protease variant having an increase in expression level in Aspergillus oryzae measured as improvement factor, EIF, of at least 1.38.