Patent ID: 11926872
Assignee: nan
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 3:
4. A method of performing isothermal amplification of a target gene to form a tandem repeat amplification product with a sense strand and an antisense strand in series on the same strand for a plurality of times comprising the following steps:
(S10) preparing a target gene DNA fragment, wherein the target gene DNA fragment is composed of a sense strand and an antisense strand;
(S20) adding a forward primer and a reverse primer to carry out a double primer isothermal amplification wherein, each of the primers is a sequence with an exogenous self-paired hairpin structure at the 5′ end, or a sequence with a hairpin structure matched with a source sequence in the target gene adjacent to the forward primer end at the 5′ end; the (S20) forms a diploid amplification product with the sense strand and the antisense strand in series for one time on the same strand;
(S30) adding the reverse primer again to perform a single primer isothermal amplification to form a tetraploid amplification product with the sense strand and the antisense strand in series for two times on the same strand; and
(S40) adding the reverse primer again to perform a single primer isothermal amplification, wherein (S40) is repeated N times to form a variety of polyploid amplification products with the sense strand and the antisense strand in series for a plurality of times on the same strand, wherein each of the polyploid amplification products increases exponentially, and a number ratio of each of the polyploid amplification products increases with an increase of repetition times, N≥1.