Patent ID: 11884943
Assignee: SINGULAR GENOMICS SYSTEMS, INC.
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 18:
19. A method of sequencing a nucleic acid sequence comprising:
a. hybridizing a nucleic acid template with a primer to form a primer-template hybridization complex;
b. contacting the primer-template hybridization complex with a DNA polymerase and nucleotides, wherein the DNA polymerase is the polymerase of claim 1 and the nucleotides comprise a modified nucleotide, wherein the modified nucleotide comprises a detectable label;
c. subjecting the primer-template hybridization complex to conditions which enable the polymerase to incorporate a modified nucleotide into the primer-template hybridization complex to form a modified primer-template hybridization complex;
d. detecting the detectable label; thereby sequencing a nucleic acid sequence.