Patent ID: 11948663
Assignee: NANT HOLDINGS IP, LLC
Field: Computer technology (Electrical engineering)
Classification: CPC G  C | IPC C  G

Claim 0:
1. A computer-based registry system for matching a donor tissue with a recipient tissue, comprising:
an HLA database storing digital data representing respective HLA types of a plurality of donor tissue samples, wherein the donor tissue samples are cord blood samples or bone marrow samples;
wherein the digital data representing the respective HLA types comprise omics data from the cord blood samples or bone marrow samples, and wherein each of the omics data comprise a corresponding plurality of sequence reads;
an HLA matching engine comprising at least one processor and at least one computer readable memory storing software instruction, the matching engine informationally coupled to the HLA database and wherein the at least one processor performs the following operations upon execution of the software instructions:
(i) decomposing the plurality of sequence reads into a plurality of respective sets of k-mers;
(ii) generating a composite de Bruijn graph using the plurality of respective sets of k-mers, and a reference sequence that includes a plurality of sequences of known and distinct HLA alleles;
(iii) ranking each of the known and distinct HLA alleles using a composite match score that is calculated from respective votes of the plurality of sequence reads, and wherein each vote uses k-mers that match corresponding segments in the known and distinct HLA alleles;
(iv) identifying a top ranked HLA allele as the first HLA-type of the donor;
(v) re-ranking remaining non-top-ranking known and distinct HLA alleles using an adjusted composite match score to identify an adjusted top-ranking HLA allele as the second HLA-type of the donor; and
(vi) identifying one of the donor tissue samples as being compatible with a recipient having a matching HLA type by the operations of:
decomposing the omics data for the recipient tissue into a plurality of k-mers,
using a composite match score to rank each of the stored respective HLA types, wherein the composite match score is generated on the basis of matching k-mers of the donor sample with the k-mers in the recipient omics data; and
identifying the first HLA type or second HLA type as being compatible with the recipient.