Patent ID: 11965181
Assignee: RIVER STONE BIOTECH, INC.
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 10:
11. A method for producing of a benzylisoquinoline alkaloid or a benzylisoquinoline alkaloid precursor, or both, comprising:
(a) providing a recombinant yeast cell capable of producing one or more benzylisoquinoline alkaloids or benzylisoquinoline alkaloid precursors, or both, that has reduced or eliminated enzymatic activity of Genes de Respuesta a Estres 2 (GRE2) comprising the amino acid sequence of SEQ ID NO: 7, or an amino acid sequence having at least 90% identity to SEQ ID NO: 7; and, optionally, (ii) one or more alcohol dehydrogenases or other aldehyde reductases, or a combination thereof,
(b) cultivating the recombinant yeast cell for a time sufficient for the recombinant yeast cell to produce a benzylisoquinoline alkaloid and/or a benzylisoquinoline alkaloid precursor; and, optionally,
(c) isolating the benzylisoquinoline alkaloid and/or a benzylisoquinoline alkaloid precursor from the recombinant yeast cell or from the cultivation supernatant, thereby producing a benzylisoquinoline alkaloid and/or a benzylisoquinoline alkaloid precursor.