Patent ID: 11965880
Assignee: SOMALOGIC OPERATING CO., INC.
Field: Measurement (Instruments)
Classification: CPC C  G | IPC C  G

Claim 8:
9. A method for quantifying the abundances of two or more species of target proteins in a biological sample, comprising:
capturing the target proteins by exposing the biological sample to a plurality of aptamers each configured to capture a specific protein;
forming an aptamer-containing eluate by isolating the aptamers that captured one of the target proteins in the biological sample;
forming a plurality of tri-molecular complexes, each including:
a particular aptamer present in the aptamer-containing eluate,
a first probe hybridized to a corresponding first portion of the particular aptamer, and
a second probe including a portion hybridized to a corresponding second portion of the particular aptamer, at least one DNA primer region, and an aptamer ID sequence corresponding to the particular aptamer;
amplifying the aptamer ID sequences of the second probes;
sequencing the amplified aptamer ID sequences of the second probes; and
based on the sequenced aptamer ID sequences, quantifying the abundances of the target proteins.