Patent ID: 11884907
Assignee: TOYO SEIKAN GROUP HOLDINGS, LTD.
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 0:
1. A culture bag for culturing lymphocytes, comprising:
a first film member having gas permeability required for cell culture; and
a second film member having gas permeability required for cell culture;
wherein the first film member and the second film member are formed into bag-shape;
a first surface inside the bag of the first film member is formed as an immobilized activating surface by immobilizing anti-CD3 antibodies at a concentration of 10 to 300 ng/cm2;
a second surface inside the bag of the second film member is formed as a non-immobilized proliferating surface without immobilizing anti-CD3 antibodies; and
a gas is sealed in an amount of 0.01 to 4 ml per cm2 of a bottom surface area in the culture bag.