Patent ID: 11926676
Assignee: CRISPR THERAPEUTICS AG
Field: Pharmaceuticals (Chemistry)
Classification: CPC C  A | IPC A  C

Claim 0:
1. A masked chimeric antigen receptor (CAR) specific to tyrosine-protein kinase-like 7 (PTK7), the masked CAR comprising:
(i) an extracellular antigen binding domain, which comprises a single chain variable fragment (scFv) that binds PTK7 and a mask peptide linked to the N-terminus of the scFv via a protease cleavage site; and
(ii) one or more intracellular signaling domains,
wherein the mask peptide comprises the amino acid sequence selected from the group consisting of:, (a)

(SEQ ID NO: 1)

EVAPGKRWFYNHVKQVPHLV,

 

(b)

(SEQ ID NO: 2)

HEEVHMRPNKLSLTWAYTGPQLR,, and
(c) X1CX2X3X4X5X6X7X8X9X10X11X12X13, in which X1 is V, W, or absent; X2 is T, H, or Y; X3 is M, F, Y, I, or H; X4 is P, G, or V; X5 is P, N, S, Y, K, L, V, or A; X6 is S, T, W, A, H, R, or Q; X7 is P, T, V, H, I, M, A, F, or W; X8 is R, M, A, H, V, Y, or absent; X9 is S, Q, Y, T, P, A, M, or I; X10 is K, R, I, C, S, Q, H, or absent; X11 is V, T, R, L, F, W, or A; X12 is I, F, L, W, or H; and X13 is C, I, or M.