Patent ID: 11937586
Assignee: OZGENE HOLDINGS PTY LTD
Field: Biotechnology (Chemistry)
Classification: CPC A  C | IPC A  C

Claim 0:
1. A method of producing male mouse embryos, wherein each and every male mouse embryo produced is infertile, the method comprising:
crossing: 1) a first mouse breeding partner carrying a disruptable transgene that comprises a GILZ gene that is operably connected to a promoter and to recombinase recognition sites that mediate disruption of the GILZ gene in the presence of a recombinase, wherein disruption of the GILZ gene has been predetermined to lead to male infertility in a mouse of a genetic background, and wherein the first mouse breeding partner is female and is homozygous for the disruptable transgene;
with 2) a second mouse breeding partner carrying a disruptor transgene that comprises a disruptor nucleotide sequence that is operably connected to a promoter and that encodes the recombinase that mediates disruption of the GILZ gene of the disruptable transgene, wherein the second mouse breeding partner is male and is homozygous for the disruptor transgene,
to thereby generate a male mouse embryo that comprises germ cells having the GILZ gene disrupted therein, wherein mouse embryos produced from the crossing are of the genetic background in which disruption of the GILZ gene has been predetermined to lead to male infertility, and wherein each and every male mouse embryo produced from the crossing is infertile; and
isolating the male mouse embryo.