Patent ID: 11913081
Assignee: THE UNIVERSITY OF CHICAGO
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 12:
13. A system comprising:
a) a first polymerase domain attached to a first interaction component;
b) a second polymerase domain attached to a second interaction component;
c) a nucleic acid construct comprising a reporter gene under the control of a first heterologous promoter responsive to polymerase activity from the first and second polymerase domains when the first and second interaction components interact, wherein the first polymerase domain comprises amino acids 1-179 of SEQ ID NOs:4, 6, or 8, and the second polymerase domain comprises the amino acid sequence of SEQ ID NO:2; and wherein the system further comprises a negative control nucleic acid encoding a polypeptide that does not interact with the first or second interaction component; and
wherein the polypeptide encoded by the negative control nucleic acid is attached to an alternate first polymerase domain comprising amino acids 1-179 of a T7 RNA polymerase or an alternate second polymerase domain comprise, comprising amino acids of a T7 RNA polymerase and the system further comprises a second heterologous promoter responsive to polymerase activity from the interaction of the first and alternate second polymerase domains or the alternate first and the second polymerase domains.