Patent ID: 11884954
Assignee: HUBEL UNIVERSITY
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 0:
1. A method for preparing a multienzyme complex system, comprising:
connecting a carbohydrate-binding module 3a (CBM3a), an immunity 2 (Im2) protein, an Im7 protein, an Im8 protein, and an Im9 protein in series through linkers to obtain a scaffold protein;
forming interaction pairs by connecting a CL2 protein, a CL7 protein, a CL8 protein and a CL9 protein respectively with the Im2 protein, the Im7 protein, the Im8 protein, and the Im9 protein on the scaffold protein;
connecting target proteins to the CL2 protein, the CL7 protein, the CL8 protein and the CL9 protein respectively to realize an orderly assembly of the target proteins on the scaffold protein, so as to obtain the multienzyme complex system;
wherein the amino acid sequence of the CL2 protein is shown in SEQ ID NO: 2;
wherein the amino acid sequence of the CL7 protein is shown in SEQ ID NO: 5;
wherein the amino acid sequence of the CL8 protein is shown in SEQ ID NO: 8;
wherein the amino acid sequence of the CL9 protein is shown in SEQ ID NO: 11;
wherein the amino acid sequence of the Im2 protein is shown in SEQ ID NO: 3;
wherein the amino acid sequence of the Im7 protein is shown in SEQ ID NO: 6;
wherein the amino acid sequence of the Im8 protein is shown in SEQ ID NO: 9; and
wherein the amino acid sequence of the Im9 protein is shown in SEQ ID NO: 12.