Patent ID: 11932869
Assignee: EYECURE THERAPEUTICS, INC. (JIANGSU)
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 0:
1. A method for digesting retinal pigment epithelium (RPE) cells to dissociate adherent RPE cells, comprising the steps of:
(1) covering RPE cells evenly with a recombinant trypsin-like enzyme and incubating;
(2) discarding the recombinant trypsin-like enzyme; and
(3) covering RPE cells evenly with neutral protease Dispase II solution and incubating;
wherein steps (1) to (3) are applied step by step; and
wherein the concentration of the neutral protease Dispase II is from 0.5 mg/mL to 4.0 mg/mL.