Patent ID: 11970734
Assignee: ILLUMINA, INC.
Field: Measurement (Instruments)
Classification: CPC C  G | IPC C  G

Claim 0:
1. A composition including:
a substrate having a first side and a second side,
a nanopore extending through the first and second sides of the substrate, wherein the nanopore has a diameter D1, and wherein nanopore comprises a constricting region that has a diameter D2 at the narrowest point, and wherein D2<D1,
a plurality of nucleotides, wherein each of the nucleotides comprises an elongated tag;
first and second polynucleotides, the first polynucleotide being complementary to the second polynucleotide;
a polymerase disposed adjacent to the first side of the nanopore, wherein the polymerase adds nucleotides of the plurality of nucleotides to the first polynucleotide based on a sequence of the second polynucleotide;
a permanent tether including a head region, a tail region, and an elongated body disposed therebetween, the head region being anchored to the polymerase, wherein the elongated body is disposed in the nanopore;
a first moiety disposed on the elongated body, wherein the first moiety is configured to bind to the elongated tag of a first nucleotide upon which the polymerase is acting; and
a reporter region disposed on the elongated body, wherein the reporter region is configured to indicate when the first nucleotide is complementary or is not complementary to a next nucleotide in the sequence of the second polynucleotide,
wherein the first moiety is configured to generate a first signal state responsive to the polymerase acting upon the first nucleotide, the first nucleotide being identifiable based on the first signal state, and
wherein the reporter region is configured to generate a second signal state when the first nucleotide is complementary or is not complementary to the next nucleotide of the second polynucleotide.