Patent ID: 11879829
Assignee: BECTON, DICKINSON AND COMPANY
Field: Measurement (Instruments)
Classification: CPC G | IPC G

Claim 5:
6. The system according to claim 5, wherein the spillover spreading coefficient is calculated according to Equation 1:, SS
  =
  
   
    
     Δ
     ⁢
     
      σ
      f
     
    
    
     
      Δ
      ⁢
      d
     
    
   
   =
   
    
     
      
       
        (
        
         σ
         
          p
          ⁢
          o
          ⁢
          s
         
        
        )
       
       2
      
      -
      
       
        (
        
         σ
         
          n
          ⁢
          e
          ⁢
          g
         
        
        )
       
       2
      
     
    
    
     
      
       d
       
        p
        ⁢
        o
        ⁢
        s
       
      
      -
      
       d
       
        n
        ⁢
        e
        ⁢
        g
       
      
     
    
   
  
 

wherein:
SS is the spillover spreading coefficient;
Δσf is an incremental standard deviation indicating the spread of the emission between the positive and negative fluorescent flow cytometer data collected from a fluorochrome; and
Δd is a difference in the intensity of the fluorescent light between the positive and negative fluorescent flow cytometer data received by a fluorescent light detector.