Patent ID: 11859248
Assignee: IMBA—INSTITUT FÜR MOLEKULARE BIOTECHNOLOGIE GMBH
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 0:
1. A method of identifying a polynucleic acid (PNA) comprising the steps of: providing a PNA; modifying one or more nucleobases of the PNA by incorporation of a thiol modified nucleobase into the PNA through biosynthesis in a cell; and further comprising alkylating said thiol modified nucleobase with an alkylating agent that comprises a hydrogen bonding partner, thereby altering the base pairing capacity of the one or more nucleobases; base pairing a complementary nucleic acid to the PNA, comprising base pairing to at least one modified nucleobase; identifying the sequence of the complementary nucleic acid at least at the position that is complementary to at least one modified nucleobase.