Patent ID: 11866745
Assignee: PAIRWISE PLANTS SERVICES, INC.
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 13:
14. A Type V Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)-associated (Cas) (CRISPR-Cas) system comprising:
a guide nucleic acid comprising a spacer sequence and a repeat sequence, and
(a) a fusion protein comprising the modified LbCas12a polypeptide of claim 1 and a polypeptide of interest, or
(b) a nucleic acid encoding the modified LbCas12a polypeptide of claim 1 and a nucleic acid encoding a polypeptide of interest, wherein the guide nucleic acid is capable of forming a complex with the modified LbCas12a polypeptide or the fusion protein and the spacer sequence is capable of hybridizing to a target nucleic acid, thereby guiding the modified LbCas12a polypeptide and the polypeptide of interest to the target nucleic acid, whereby the target nucleic acid is modified or modulated.