Patent ID: 11969480
Assignee: TRANSLATE BIO, INC.
Field: Pharmaceuticals (Chemistry)
Classification: CPC A  B  C | IPC A  B  C

Claim 7:
8. A method of manufacturing a lipid nanoparticle (LNP) composition, the method comprising:
(a) mixing a first set of preformed empty lipid nanoparticles (LNPs) comprising a first cationic lipid, a first non-cationic lipid, a first PEG-modified lipid, and optionally cholesterol with mRNA under conditions that allow encapsulation of the mRNA;
(b) combining the mRNA-encapsulating LNPs formed in step (a) with a second set of preformed empty LNPs comprising a second cationic lipid, a second non-cationic lipid, a second PEG-modified lipid, and optionally cholesterol, resulting in the LNP composition.