Patent ID: 11920190
Assignee: INDUSTRIAL TECHNOLOGY RESEARCH INSTITUTE
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 0:
1. A method of amplifying a target nucleotide sequence, comprising the following steps:
(a) linking a first adaptor and a second adaptor to two ends of a double-stranded nucleic acid molecule with a target nucleotide sequence respectively to form a nucleic acid template, wherein the target nucleotide sequence comprises a forward strand and a reverse strand, the first adaptor comprises a Y-form adaptor or a hairpin adaptor, and the second adaptor is a hairpin adaptor, wherein the first adaptor comprises a paired double-stranded part and an unpaired single-stranded part;
(b) performing a denaturation reaction on the nucleic acid template;
(c) performing a PCR amplification on the target nucleotide sequence, so that a first primer, a second primer, and a DNA polymerase are in contact with the nucleic acid template, wherein the first primer comprises a first sequence identical to a sequence of the single-stranded part of the first adaptor near the forward strand, and the second primer comprises a second sequence complementary to a sequence of the single-stranded part of the first adaptor near the reverse strand, and wherein the second primer binds to the single-strand part of the first adaptor and does not bind to the double-stranded part of the first adaptor; and
(d) repeating step (c) to perform one or a plurality of PCR amplification cycles to obtain a PCR amplicon of the target nucleotide sequence.