Patent ID: 11932687
Assignee: KING FAISAL UNIVERSITY
Field: Measurement (Instruments)
Classification: CPC C  G | IPC C  G

Claim 0:
1. A method for antigen-capture ELISA for diagnosis of Haemonchus longistipes (H. longistipes) infection in camels, consisting of steps of:
separating a 76 kDa protein fraction from a crude extract from Haemonchus longistipes worms by Sodium dodecyl-sulfate polyacrylamide gel electrophoresis (SDS-PAGE) in a whole polyacrylamide gel stained with a light stain, the 76 kDa protein fraction providing an H. longistipes antigen;
electro-eluting the 76 kDa protein fraction from the polyacrylamide gel to provide a pure, concentrated H. longistipes antigen;
developing antibodies to H. longistipes by immunizing rabbits with the electro-eluted pure, concentrated 76 kDa H. longistipes antigen;
purifying antibodies obtained from sera of the immunized rabbits by affinity chromatography with protein A sepharose and phosphate-buffered saline to obtain purified anti-76 kDa H. longistipes protein IgG antibody molecules;
labeling the purified anti-76 kDa H. longistipes IgG antibody molecules by conjugating the IgG antibodies with horseradish periodate;
coating wells of an Enzyme-Linked Immunosorbent Assay (ELISA) microtiter plate with a 1/50 2-fold dilution of the labeled H. longistipes IgG antibodies; and
performing an antigen-capture ELISA test on sera from a camel using the ELISA microtiter plate coated with the labeled H. longistipes IgG antibodies to detect a 76 kDa H. longistipes in the camel's which provides indication of the presence of H. longistipes infection in the camel sera.