Patent ID: 11952557
Assignee: NOVOZYMES A/S
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 15:
16. The variant of claim 1, which has an IF of >1 when compared to the parent alpha-amylase, and which comprises:, N54S + V56T + G109A + A174S + G182* + D183* + N195F + V206L + I405L + A421H + A422P + 

A428T + D476K,

N54S + V56T + G109A + A174S + G182* + D183* + N195F + V206L + I405L + A421H + A422P + 

A428T,

N54S + V56T + G109A + A174S + D183* + N195F + V206L + K391A + I405L + A421H + A422P + 

A428T,

N54S + V56T + G109A + A174S + G182* + D183* + N195F + V206L + K391A + I405L + A421H + 

A422P + A428T + D476K,

N54S + V56T + G109A + A174S + G182* + D183* + N195F + V206L + K391A + I405L + A421H + 

A422P + A428T + D476G,

N54S + V56T + G109A + A174S + G182* + D183* + N195F + V206L + K391A + I405L + A421H + 

A422P + A428T + D476K + G477S,

N54S + V56T + G109A + A174S + G182* + D183* + N195F + V206L + K391A + I405L + A421H + 

A422P + A428T + D476K,

N54S + V56T + K72R + G109A + W167F + A174S + G182* + D183* + N195F + V206L + I405L + 

A421H + A422P + A428T,

N54S + V56T + G109A + A174S + G182* + D183* + N195F + V206L + S304N + I405L + A421H + 

A422P + A428T + D476K,

N54S + V56T + G109A + A174S + G182* + D183* + N195F + V206L + K391A + I405L + A421H + 

A422P + A428T + D476K + G477A,

N54S + V56T + G109A + A174S + G182* + D183* + N195F + V206L + K391A + I405L + A421H + 

A422P + A428T + D476G + G477T,

N54S + V56T + G109A + A174S + G182* + D183* + N195F + V206L + K391A + I405L + A421H + 

A422P + A428T + G477Q,

N54S + V56T + G109A + A174S + G182* + D183* + N195F + V206L + K391A + I405L + A421H + 

A422P + A428T + G477A,

N54S + V56T + G109A + A174S + G182* + D183* + N195F + V206L + K391A + I405L + A421H + 

A422P + A428T + D476K + G477Q,

N54S + V56T + G109A + A174S + G182* + D183* + N195F + V206L + K391A + I405L + A421H + 

A422P + A428T + D476N,

N54S + V56T + G109A + A174S + G182* + D183* + N195F + V206L + K391A + I405L + A421H + 

A422P + A428T + D476Y,

N54S + V56T + G109A + A174S + G182* + D183* + N195F + V206I + I405L + A421H + A422P + 

A428T,

N54S + V56T + G109A + Q172D + A174S + G182* + D183* + N195F + A204G + V206L + I405L + 

A421H + A422P + A428T,

N54S + V56T + K72R + G109A + T134E + A174S + G182* + D183* + N195F + A204G + V206L + 

G255A + I405L + A421H + A422P + A428T,

N54S + V56T + G109A + F113Q + R116H + T165G + Q172G + A174S + G182* + D183* + N195F + 

V206L + I405L + A421H + A422P + A428T,

N54S + V56T + G109A + F113Q + Q172N + A174S + G182* + D183* + N195F + V206L + I405L + 

A421H + A422P + A428T,

N54S + V56T + K72R + G109A + T134E + A174S + G182* + D183* + N195F + G196R + V206L + 

G255A + I405L + A421H + A422P + A428T + N475S,

N54S + V56T + G109A + T134E + A174S + G182* + D183* + N195F + V206L + I405L + A421H + 

A422P + A428T,

T40D + N54S + V56T + G109A + A174S + G182* + D183* + N195F + V206L + I405L + A421H + 

A422P + A428T,

N54S + V56T + G109A + F113Q + N125D + A174S + G182* + D183* + N195F + V206L + I405L + 

A421H + A422P + A428T,

N54S + V56T + A60V + G109A + R116Q + A174S + G182* + D183* + N195F + V206L + I405L + 

A421H + A422P + A428T,

N54S + V56T + G109A + R116V + A174S + G182* + D183* + N195F + V206L + I405L + A421H + 

A422P + A428T,

N54S + V56T + G109A + R116Q + G133Q + T165G + Q172G + A174S + G182* + D183* + N195F + 

V206L + I405L + A421H + A422P + A428T,

N54S + V56T + G109A + F113Q + R116H + Q172G + A174S + G182* + D183* + N195F + V206L + 

1405L + A421H + A422P + A428T,

N54S + V56T + G109A + F113Q + R116Q + Q172G + A174S + G182* + D183* + N195F + V206L + 

1405L + A421H + A422P + A428T + G448D,

N54S + V56T + G109A + F113Q + R116H + Q172N + A174S + G182* + D183* + N195F + V206L + 

1405L + A421H + A422P + A428T,

N54S + V56T + K72R + G109A + T134E + W167F + Q172N + A174S + G182* + D183* + N195F + 

V206L + G255A + A265G + I405L + A421H + A422P + A428T,

N54S + V56T + K72R + G109A + R116Q + W167F + Q172R + L173V + A174S + G182* + D183* + 

N195F + V206L + A265G + I405L + A421H + A422P + A428T,

N54S + V56T + K72R + G109A + R116Q + W167F + Q172R + A174S + G182* + D183* + N195F + 

V206L + I405L + A421H + A422P + A428T,

N54S + V56T + K72R + G109A + F113Q + T134E + W167F + Q172G + A174S + G182* + D183* + 

N195F + V206L + G255A + I405L + A421H + A422P + A428T,

N54S + V56T + K72R + G109A + F113Q + R116H + W167F + Q172G + L173V + A174S + G182* + 

D183* + G184T + N195F + V206L + I405L + A421H + A422P + A428T,

N54S + V56T + G109A + F113Q + W167F + Q172G + A174S + G182* + D183* + N195F + V206L + 

1405L + A421H + A422P + A428T,

N54S + V56T + K72R + G109A + A174S + G182* + D183* + N195F + V206L + G255A + I405L + 

A421H + A422P + A428T,

N54S + V56T + G109A + F113Q + R116Q + A174S + G182* + D183* + N195F + V206L + I405L + 

A421H + A422P + A428T,

N54S + V56T + K72R + G109A + F113Q + F116H + W167F + Q172G + L173V + A174S + G182* + 

D183* + N195F + V206L + I405L + A421H + A422P + A428T,

N54S + V56T + K72R + G109A + F113Q + R116Q + W167F + Q172G + A174S + G182* + D183* + 

G184T + N195F + V206L + I405L + A421H + A422P + A428T,

N54S + V56T + G109A + F113Q + R116Q + W167F + Q172G + A174S + G182* + D183* + 

N195F + A204G + V206L + I405L + A421H + A422P + A428T,

N54S + V56T + G109A + F113Q + R116H + W167F + Q172G + A174S + G182* + D183* + 

N195F + V206L + A265G + I405L + A421H + A422P + A428T,

N54S + V56T + G109A + R116Q + A174S + G182* + D183* + N195F + V206L + I405L + A421H + 

A422P + A428T,

N54S + V56T + A60V + G109A + R116Q + W167F + Q172N + L173V + A174S + G182* + D183* + 

N195F + V206L + I405L + A421H + A422P + A428T,

A37H + N54S + V56T + A60V + G109A + R116Q + T165G + A174S + G182* + D183* + N195F + 

V206L + I405L + A421H + A422P + A428T,

N54S + V56T + G109A + A174S + M105F + G182* + D183* + N195F + V206L + L228I + R320A + 

S323N + I405L + A421H + A422P + A428T,

N54S + V56T + K72R + G109A + F113Q + T134E + A174S + G182* + D183* + N195F + V206L + 

G255A + I405L + A421H + A422P + A428T,

N54S + V56T + K72R + G109A + R116Q + V120L + A174S + G182* + D183* + G184T + N195F + 

V206L + I405L + A421H + A422P + A428T,

N54S + V56T + G109A + F113Q + R116H + W167F + Q172G + L173V + A174S + G182* + 

D183* + N195F + V206L + I405L + A421H + A422P + A428T,

N54S + V56T + G109A + F113Q + R116Q + Q172N + A174S + G182* + D183* + N195F + V206L + 

A265G + I405L + A421H + A422P + A428T, or

A37H + N54S + V56T + A60V + G109A + R116Q + W167F + Q172R + A174S + G182* + D183* + 

N195F + V206L + I405L + A421H + A422P + A428T,

wherein the positions correspond to SEQ ID NO: 14.