Patent ID: 11920183
Assignee: 10X GENOMICS, INC.
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 0:
1. A method for processing a bead, comprising:
(i) hybridizing the bead and a modified probe to generate an optically tagged bead, wherein the bead comprises a binding sequence, wherein the modified probe hybridizes to the binding sequence, wherein the modified probe comprises a fluorescent tag and a 3′ chain-terminating modification at a 3′ end of the modified probe, and wherein the 3′ chain-terminating modification at the 3′ end of the modified probe renders the modified probe hybridized to the bead at least 60% less reactive in an extension reaction as compared to an equivalent unmodified nucleic acid probe; and
(ii) detecting one or more signals indicative of the fluorescent tag from the optically tagged bead,, wherein the optically tagged bead comprises: i) a molecule comprising the binding sequence hybridized to the modified probe, and ii) a nucleic acid barcode molecule, wherein the molecule is different from the nucleic acid barcode molecule.