Patent ID: 11860100
Assignee: UNIVERSITY OF ROCHESTER
Field: Measurement (Instruments)
Classification: CPC G | IPC G

Claim 0:
1. A method of staining a sample for examination by an ultraviolet surface excitation microscopy system, the method comprising:
(a) selecting a mixed fluorescence dye solution made from a set of fluorescing stains in preselected proportions for generating the mixed fluorescence dye solution when dissolved in a preselected volume of a solvent, the solution comprising:
(i) at least a first fluorescing stain at a first concentration and a second fluorescing stain at a second concentration,
(ii) the first fluorescing stain characterized by a first light emission spectrum when subjected to an ultraviolet light of a first wavelength by the ultraviolet surface excitation microscopy system, the second fluorescing stain characterized by a second light emission spectrum when subjected to the first ultraviolet light source wavelength, wherein the first emission spectrum is different from the second emission spectrum; and

(b) exposing a sample for ultraviolet surface excitation microscopy examination to the mixed fluorescence dye solution for a predetermined time period to stain features of the sample;
wherein the selected mixed fluorescent dye solution is configured to prepare the sample for imaging by the ultraviolet surface excitation microscopy system at the first ultraviolet light source wavelength such that light emissions of features associated with the first fluorescing stain are imaged by the system at a first intensity and the light emissions of features associated with the second fluorescing stain are imaged by the system at a second intensity, wherein the first and second intensities are approximately the same.