Patent ID: 11932861
Assignee: INDUSTRY-ACADEMIC COOPERATION FOUNDATION GYEONGSANG NATIONAL UNIVERSITY
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 0:
1. A recombinant vector for genome editing without inserting a replicon into plant genome in a T0 generation plant, the recombinant vector including a geminivirus-based replicon between the sequence of LB (left border) and sequence of RB (right border) of Ti plasmid,
wherein the geminivirus is BeYDV (Bean Yellow Dwarf Virus),
wherein the replicon has a first long intergenic region (LIR), a Rep/RepA protein-coding sequence, a short intergenic region (SIR), a CaMV 35S terminator, a Cpf1 coding sequence, a CaMV 35s promoter having AtUBQ intron, U6-double gRNAs, a first template DNA site for gene-editing, a NOS promoter, NPTII coding sequence, a OCS (octopine synthase) terminator, a CaMV 35S promoter, a second template DNA site for gene-editing, and a second long intergenic region (LIR) that are sequentially connected.