Patent ID: 11903392
Assignee: FRIESLANDCAMPINA NEDERLAND B.V.
Field: Food chemistry (Chemistry)
Classification: CPC A | IPC A

Claim 0:
1. A method of manufacturing a hypoallergenic nutritional composition, comprising:
(a) providing a partial hydrolysate of milk protein(s) by subjecting a starting composition comprising one or more bovine milk protein(s) in an aqueous medium to an enzymatic treatment, the partial hydrolysate having a degree of hydrolysis (DH) in the range of 5 to 20%,
(b) clearing from the partial hydrolysate of milk protein(s) one or more aggregates capable of binding to receptor for advanced glycation end products (RAGE) and/or having a basophil degranulation inducing capacity, thereby forming a cleared partial hydrolysate; and
(c)formulating the cleared partial hydrolysate into a hypoallergenic nutritional composition for infants;
wherein the clearing comprises:
(i) filtering the partial hydrolysate using a membrane having a molecular cut-off in the range of 50-100 kDa; or
(ii) subjecting the partial hydrolysate to size exclusion chromatography thereby removing aggregates having a molecular weight of 100 kDa or higher.