Patent ID: 11972535
Assignee: STELLENBOSCH UNIVERSITY
Field: Measurement (Instruments)
Classification: CPC G | IPC G

Claim 8:
9. A computer system comprising:
a processor and a memory configured to provide computer program instructions to the processor to execute functions of components:
an accessing component for accessing multi-dimensional signal intensity data in the form of fluorescence data in a three-dimensional image space including signal intensity data for a first fluorescence channel and a second fluorescence channel associated with each voxel of a three-dimensional image;
a regression calculating component for calculating a regression as an errors-in-variables regression on the fluorescence data to generate a regression parameter corresponding to a degree of correlation between the signal intensity data obtained from the first and second fluorescence channels, with values of the regression parameter represented by a best fit line of points on a graph with axes representing the signal intensity values of the first and second fluorescence channels and with colourmap values varying along the regression parameter line;
a mapping component for mapping, for each voxel, the in the three-dimensional image space signal intensity to the regression parameter line;
a colourmap value assigning component for assigning a colourmap value to each such voxel in the three-dimensional space based on the mapping; and
a rendering component for rendering the voxels in the three-dimensional image for display on a computer display in colours related to the assigned colourmap values resulting in a visualisation of colocalisation of the fluorescence data directly in the three-dimensional image space.