Patent ID: 11911415
Assignee: EDITAS MEDICINE, INC.
Field: Pharmaceuticals (Chemistry)
Classification: CPC A  C  G | IPC A  C  G

Claim 7:
8. The method of claim 7, wherein the step of selecting the first allele-specific gRNA molecule using a database schema comprises:
receiving, via an interface of a computational system, a listing of a first plurality of alleles of the endogenous HLA gene of a first subject;
receiving, via the interface of the computational system, a listing of a second plurality of alleles of the endogenous HLA gene of a second subject;
processing the listings of the first and the second pluralities of alleles to identify one or more mismatched alleles between the first plurality of alleles and the second plurality of alleles;
querying a database to determine whether one or more gRNA molecules are suitable for editing the one or more mismatched alleles of the second plurality of alleles;
generating a list of gRNA molecules that identifies the one or more gRNA molecules found to be suitable;
ranking the list of gRNA molecules; and
displaying the ranked list of gRNA molecules.