Patent ID: 11926926
Assignee: TAKEDA PHARMACEUTICAL COMPANY LIMITED
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 0:
1. A method of diversifying a library, the method comprising mutagenizing a focused library of vectors or genetic packages that display, display and express, or comprise a member of a diverse family of human antibody related peptides, polypeptides and proteins and collectively display, display and express, or comprise at least a portion of the diversity of the antibody family, wherein the vectors or genetic packages comprise variegated DNA sequences that encode a heavy chain (HC) CDR3 selected from the group consisting of:
(a) a HC CDR3 that is about 3 or about 4 or about 5 amino acids in length;
(b) a HC CDR3 that is about 23, about 24, about 25, about 26, about 27, about 28, about 29, about 30, about 31, about 32, about 33, about 34 or about 35 amino acids in length (e.g., about 23 to about 35 amino acids in length); and
c) a HC CDR3 that is from about 6 to about 20 amino acids in length,
wherein the HC CDR3 comprises amino acids from a diversified D region or fragment thereof or an extended JH region.