Patent ID: 11957324
Assignee: ETHICON, INC.
Field: Pharmaceuticals (Chemistry)
Classification: CPC A  C | IPC A

Claim 0:
1. A method for preparing and delivering a fibrin sealant to a surface, the method comprising:
a. providing a quantity of a cell-free liquid mixture comprising:
I. fibrin or
II. fibrinogen and Factor II; and

b. passing the mixture through a resin bed comprising a size-exclusion chromatographic resin pre-equilibrated with calcium so as to (i) reduce a concentration of a small molecule inhibitor(s) of fibrin clot formation within the mixture and/or (ii) increase a concentration of the calcium within the mixture, so that after the passage of the mixture through the resin, a fibrin clot is formed with a clotting time (TCLOT) of at most 1 hour at an ambient temperature selected from the group consisting of 21° C., 22° C., 23° C., 24° C., and 25° C.