Patent ID: 11884935
Assignee: KOREA INSTITUTE OF SCIENCE AND TECHNOLOGY
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 0:
1. An in vitro method of inducing trans-differentiation of a fibroblast to a cardiomyocyte, the method comprising:
a) isolating first exosomes isolated from stem cells during a mesoderm induction process and second exosomes isolated from the stem cells during a cardiac specification and maturation process;
b) culturing a fibroblast in a cardiomyocyte reprogramming medium containing the first exosomes; and
c) culturing the fibroblast in a cardiomyocyte differentiation medium containing the second exosomes isolated from the step a);
wherein the first exosomes comprise miR-291a-3p, miR-292a-3p, miR-292a-5p, miR-293-3p, miR-293-5p, miR-295-3p, miR-294-3p, miR-292b-5p, miR-148a-3p, miR-363-3p, miR429-3p, miR291a-5p, miR-200b-3p, miR-672-5p, miR-335-3p, miR-302b-3p, miR-200a-5p, miR-881-3p, and miR-135b-5p, and the expression of miR-291a-3p, miR-292a-3p and miR-292a-5p is more than twice that of the fibroblast;
wherein the second exosomes comprise miR-1a-3p, miR-302d-3p, miR-292a-3p, miR-291a-ep, miR-302c-3p, miR-335-3p, miR-431-5p, miR-376b-3p, miR-434-3p, miR-1b-5p, miR-434-3p, miR-293-3p, miR-291a-5p, miR-302b-3p, miR-126a-5p, miR-355-5p, miR-126b-3p, miR-148a-3p, miR-292b-3p, miR-292a-5p and miR-363-3p, and the expression of miR-1a-3p and miR-302d-3p is more than twice that of the fibroblast; and
wherein the fibroblast is isolated from the same species as the stem cells.