Patent ID: 11921049
Assignee: nan
Field: Measurement (Instruments)
Classification: CPC C  G | IPC C  G

Claim 1:
2. The plurality of pairs of forward and reverse 3′ blocked primers for multiplex pyrophosphorolysis activated polymerization of claim 1, wherein the first forward or reverse blocked primer has a FAM (carboxyfluorescein) fluorophore attached to a nucleotide in the internal region or at the 5′ end and a universal TAMRA (carboxytetramethylrhodamine) quencher attached to the first non-extendable, blocked nucleotide at the 3′ end, wherein the first non-extendable, blocked nucleotide is a dideoxynucleotide, and wherein the second forward or reverse blocked primer has a HEX (hexachlorofluorescein) fluorophore attached to a nucleotide in the internal region or at the 5′ end and the same universal TAMRA (carboxytetramethylrhodamine) quencher attached to the second non-extendable, blocked nucleotide at the 3′ end, wherein the second non-extendable, blocked nucleotide is a dideoxynucleotide.