Patent ID: 11970533
Assignee: SPARX BIOSCIENCE LIMITED
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 9:
10. The bispecific antibody of claim 4, wherein a disulfide bond is formed between a variable region of the first antigen-binding moiety and a variable region in the second antigen-binding moiety, wherein:
(a) the first VL or the second VL contains C at position 43 (C43) and the first VH or the second VH contains C at position 105 (C105) corresponding to the Kabat numbering; and wherein the disulfide bond is formed between C43 in the first VL and C105 in the first VH, or between C43 in the second VL and C105 in the second VH;
(b) the first VL or the second VL contains C at position 100 (C100) and the first VH or the second VH contains C at position 44 (C44) corresponding to the Kabat numbering; and wherein the disulfide bond is formed between C100 in the first VL and C44 in the first VH, or between C100 in the second VL and C44 in the second VH;
(c) the first VH contains C at position 3 (C3) or position 9 (C9) and the second VH contains C at position 42 (C42) or position 112 (C112) corresponding to the Kabat numbering, or vice versa, and wherein the disulfide bond is formed between C3 and C42, or C9 and C112;
(d) the first VL contains C at position 43 (C43) and the first VH contains C at position 105 (C105) corresponding to the Kabat numbering; and wherein the disulfide bond is formed between C43 and C105;
(e) the second VL contains C at position 43 (C43) and the second VH contains C at position 105 (C105) corresponding to the Kabat numbering; and wherein the disulfide bond is formed between C43 and C105;
(f) the second VH contains C at position 3 (C3) and the first VH contains C at position 42 (C42) corresponding to the Kabat numbering; and wherein the a disulfide bond is formed between C3 and C42;
(g) the second VH contains C at position 9 (C9) and the first VH contains C at position 112 (C112) corresponding to the Kabat numbering; and wherein the disulfide bond is formed between C9 and C112;
(h) the second VH contains C at position 44 (C44) and the second VL contains C at position 100 (C100) corresponding to the Kabat numbering; and wherein the disulfide bond is formed between C44 and C100;
(i) the first VH contains C at position 44 (C44) and the first VL contains C at position 100 (C100) corresponding to the Kabat numbering; and wherein the disulfide bond is formed between C44 and C100;
(j) the second VH contains C at positon 105 (C105) and the second VL contains C at position 43 (C43) corresponding to the Kabat numbering; and wherein the disulfide bond is formed between C105 and C43; or
(k) the first VH contains C at position 105 (C105) and the first VL contains C at position 43 (C43) corresponding to the Kabat numbering; and wherein the disulfide bond is formed between C105 and C43.