Patent ID: 11939606
Assignee: LOCANABIO, INC.
Field: Biotechnology (Chemistry)
Classification: CPC C  Y | IPC C

Claim 10:
11. A method of directing a Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)-associated (Cas) nucleoprotein complex to a selected nucleic acid target sequence, the method comprising:
contacting the selected nucleic acid target sequence with one or more nucleoprotein complexes comprising:
a Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)-associated (Cas) protein, wherein the Cas protein has at least 95 percent sequence identity to the amino acid sequence of SEQ ID NO: 40; and
a cognate nucleic acid guide comprising a repeat sequence and a spacer sequence, wherein the repeat sequence and the spacer sequence do not naturally occur together, wherein the Cas protein and the cognate nucleic acid guide form the nucleoprotein complex, and wherein the nucleoprotein complex binds to the selected nucleic acid target sequence.