Patent ID: 11898169
Assignee: THE MCLEAN HOSPITAL CORPORATION
Field: Pharmaceuticals (Chemistry)
Classification: CPC C  A | IPC A  C

Claim 0:
1. A method of generating mammalian patient-specific induced pluripotent stem cells for treating a patient, comprising:
providing a quantity of mammalian somatic or non-embryonic cells from the patient;
contacting the mammalian somatic or non-embryonic cells with a quantity of one or more reprogramming factors and one or more microRNA molecules; and
culturing the somatic or non-embryonic cells for a period of time sufficient to generate at least one induced pluripotent stem cell,
wherein the one or more microRNA molecules consist of (i) at least one miR-302 cluster member and (ii) at least one miR-200 cluster member, and
wherein the quantity of reprogramming factors comprises at least Oct-4, Sox-2, Klf-4, and at least one of c-Myc or 1-Myc.