Patent ID: 11884945
Assignee: TAKEDA PHARMACEUTICAL COMPANY LIMITED
Field: Pharmaceuticals (Chemistry)
Classification: CPC C  A | IPC A  C

Claim 0:
1. A method of purifying recombinant arylsulfatase A (ASA) protein, the method comprising
purifying recombinant arylsulfatase A (ASA) protein from an impure preparation by conducting two or more chromatography steps;
pooling eluate from the final chromatography steps;
adjusting the pH of the pooled elute to between 5.8 to 6.4; and
subjecting the pH-adjusted eluate to a single step of post chromatographic ultrafiltration and/or diafiltration,
thereby exchanging the purified recombinant ASA protein directly into a saline-based formulation, and
wherein the method does not comprise more than one ultrafiltration and/or diafiltration step.