Patent ID: 11891593
Assignee: THE GENERAL HOSPITAL CORPORATION
Field: Pharmaceuticals (Chemistry)
Classification: CPC C  A | IPC A  C

Claim 7:
8. A method comprising:
providing:
(i) a lung tissue matrix including an airway and substantial vasculature;
(ii) an organ chamber configured to hold the lung tissue matrix, and
(iii) a media reservoir system;

connecting the organ chamber to the media reservoir system via an ingress line and an egress line, wherein the ingress line comprises an ingress pump and wherein the egress line comprises a bidirectional egress pump; and
maintaining a substantially constant fluid level in the organ chamber during negative pressure wet ventilation, wherein the maintaining the substantially constant fluid level comprises:
monitoring pressure in the organ chamber using a chamber pressure sensor and a controller, wherein the chamber pressure sensor is connected to the organ chamber and configured to record and transmit a pressure reading at the organ chamber to the controller, and
correcting for fluid shifts through the lung tissue matrix into the organ chamber by adjusting direction and duration of the bidirectional egress pump.