Patent ID: 11866703
Assignee: LANZHOU VETERINARY RESEARCH INSTITUTE, CHINESE ACADEMY OF AGRICULTURAL SCIENCES
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 9:
10. The knockout method according to claim 9, wherein,
a) the PCR1 primer pair comprises PCR1-F and PCR1-R, the PCR1-F has the nucleotide sequence shown in SEQ ID NO: 3, and the PCR1-R has the nucleotide sequence shown in SEQ ID NO: 4;
b) the PCR2 primer pair comprises PCR2-F and PCR2-R, the PCR2-F has the nucleotide sequence shown in SEQ ID NO: 5, and the PCR2-R has the nucleotide sequence shown in SEQ ID NO: 6; and
c) the PCR3 primer pair comprises PCR3-F and PCR3-R, the PCR3-F has the nucleotide sequence shown in SEQ ID NO: 7, and the PCR3-R has the nucleotide sequence shown in SEQ ID NO: 8.