Patent ID: 11970533
Assignee: SPARX BIOSCIENCE LIMITED
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 24:
25. A bispecific antibody, comprising a first antigen-binding moiety that binds human claudin 18.2, and a second antigen-binding moiety that binds human PD-L1, wherein the first antigen-binding moiety and the second antigen-binding moiety are located on a single polypeptide,
wherein the first antigen-binding moiety that binds human claudin 18.2 comprises a first heavy chain variable region (VH) and a first light chain variable region (VL), and wherein:
the first VL comprises the amino acid sequence of:
(i) DIQMTQSPSSLSASVGDRVTITCKSSQSLLNX1GNQKSYLTWYQQKPGKX2PK LLIYWASTLX3SGVPSRFSGSGSGTDYTLTISSLQPEDFATYYCQNAYFFPFTFGX4GTKVEIK (SEQ ID NO 167), in which X1 represents Serine (S), or Tryptophan (W); X2 represents Alanine (A), or Cysteine (C); X3 represents Glutamic acid (E), or Glutamine (Q); and X4 represents Glutamine (Q), or Cysteine (C); or
(ii) DIQMTQSPSSLSASVGDRVTITCKSSQSLLNSGNQKSYLTWYQQKPGKX1PKL LIYWASTLESGVPSRFSGSGSGTDYTLTISSLQPEDFATYYCQNAYFFPFTFX2 (SEQ ID NO: 169, in which X1 represents Alanine (A), or Cysteine (C); X2 represents Glutamine (Q), or Cysteine (C); and

the first VH comprises the amino acid sequence of:
(i) EVQLVESGGGLVQPGGSLRLSCAVSGYTFSMNWVRQAPX1KX2LEWVAWIN MYTGEX3TYADDFKGRFTISRDDSKNTLYLQMNSLRAEDTAVYYCARLYNGNSLDYWGX4GTLVTVX5S (SEQ ID NO 166), in which X1 represents Glycine (G), or Cysteine (C); X2 represents Glycine (G), or Cysteine (C); X3 represents Proline (P) or Arginine (R); X4 represents Glutamine (Q), or Cysteine (C); and X5 represents Serine (S), or Cysteine (C); or
(ii) EVQLVESGGGLVQPGGSLRLSCAVSGYTFSMNWVRQAPX1KX2LEWVAWIN MYTGEPTYADDFKGRFTISRDDSKNTLYLQMNSLRAEDTAVYYCARLYNGNSLDYWGX3GTLVTVX4S (SEQ ID NO 168), in which X1 represents amino acid residues Glycine (G), or Cysteine (C); X2 represents amino acid residues Glysine (G), or Cysteine (C), X3 represents amino acid residues Glutamine (Q), or Cysteine (C); and X4 represents amino acid residues Serine (S), or Cysteine (C).