Patent ID: 11921023
Assignee: NEW YORK UNIVERSITY
Field: Measurement (Instruments)
Classification: CPC G | IPC G

Claim 0:
1. A method of characterizing particles, comprising:
dispersing within a fluid medium a plurality of porous particles, having a known diameter and refractive index, and a plurality of unknown particles to form a mixture;
flowing a sample of the mixture through an observation volume of a holographic microscope;
generating a first holographic image based upon holographic video microscopy of the sample within the observation volume at a first time;
analyzing the first holographic image for one or more regions of interest corresponding to a porous particle of interest;
normalizing the one or more regions of interest for a contribution of a wave created by interaction of light with the sample;
fitting the normalized region of interest to a light scattering theory; and
characterizing the refractive index of the porous particle of interest;
adding a miscible fluid to the sample mixture, forming a modified mixture;
flowing a modified mixture sample through the observation volume of the holographic microscope;
generating a second holographic image based upon holographic video microscopy of the modified mixture sample within the observation volume at a second time;
analyzing the second holographic image for one or more second regions of interest corresponding to a second porous particle of interest;
normalizing the one or more second region of interest for a contribution of a wave created by interaction of light with the modified mixture sample;
fitting the normalized second region of interest to the light scattering theory; and
characterizing the refractive index of the second porous particle of interest;
comparing the refractive index of the second porous particle of interest to the refractive index of the porous particle of interest; and
determining by the comparison of refractive indices if the unknown particle is present in pores of the plurality of porous particles.