Patent ID: 11859237
Assignee: ACADEMIA SINICA
Field: Measurement (Instruments)
Classification: CPC C  B  G | IPC B  C  G

Claim 0:
1. A method for sizing a DNA molecule, comprising the following steps of:
providing a DNA sizing device, comprising:
a cover substrate;
a substrate disposed on the cover substrate and comprising a first hole and a second hole; and
a first slit-like channel disposed between the cover substrate and the substrate, wherein two ends of the first slit-like channel respectively connects to the first hole and the second hole;

loading a sample comprising a DNA molecule to the first slit-like channel through the first hole, wherein the DNA molecule moves in a direction from the first hole to the second hole;
taking a first image related to a distribution of the DNA molecule;
detecting and recording first intensity values of the DNA molecule and a first area of distribution which corresponds to an area of all the detected first intensity values of the DNA molecule which is detected from the first image; and
analyzing the first intensity values and the first area to obtain a size of the DNA molecule.