Patent ID: 11951171
Assignee: TIANJIN MEDICAL UNIVERSITY
Field: Pharmaceuticals (Chemistry)
Classification: CPC A  C | IPC A  C

Claim 15:
16. The disease diagnosis kit according to claim 15, the reagent for extracting and purifying the exosome includes: a nickel bead coated with the polypeptide EXP; a binding buffer of pH 7.4 comprising 50 mM imidazole, 500 mM sodium chloride, and 20 mM disodium hydrogen phosphate; a washing solution of pH 7.4 comprising 75 mM imidazole, 500 mM sodium chloride, and 20 mM disodiumhydrogen phosphate; and an eluent of pH 7.4 comprising 500 mM imidazole, 500 mM sodium chloride, and 20 mM disodium hydrogen phosphate wherein the exosome is selected from the group consisting of a free exosome derived from a source other than a cell culture supernatant and a free exosome derived from a cell culture supernatant.