Patent ID: 11862296
Assignee: NATIONAL KAOHSIUNG UNIVERSITY OF SCIENCE AND TECHNOLOGY
Field: Computer technology (Electrical engineering)
Classification: CPC G | IPC G

Claim 3:
4. The method for designing the multi-objective primer pair as claimed in claim 1, wherein the at least two objectives include the third objective calculating a difference between the melting temperature of the forward primer and the melting temperature of the reverse primer and taking an absolute value thereof to obtain a first difference value, calculating a difference between the optimal melting temperature of the forward primer and the optimal melting temperature of the optimal reverse primer and taking an absolute value thereof to obtain a second difference value, wherein if the first difference value is not greater than the second difference value, the third objective is equal to 0, and if the first difference value is greater than the second difference value, the third objective is equal to a difference between the first difference value and the second difference value.