Patent ID: 11883439
Assignee: SYNLOGIC OPERATING COMPANY, INC.
Field: Pharmaceuticals (Chemistry)
Classification: CPC A  C  Y | IPC A  C

Claim 0:
1. A method of producing a pharmaceutically acceptable composition comprising a genetically-engineered non-pathogenic bacterium for use in metabolizing phenylalanine, the bacterium comprising
at least one heterologous gene encoding a phenylalanine transporter for importing phenylalanine into the bacterium operably linked to a directly or indirectly inducible promoter that is not associated with the phenylalanine transporter in nature, and
at least one heterologous gene encoding a polypeptide involved in metabolizing phenylalanine operably linked to a directly or indirectly inducible promoter that is not associated with the at least one heterologous gene encoding the polypeptide in nature,
the method comprising the steps of:
a) growing the bacterium in a growth medium culture under conditions that do not induce the promoter that controls expression of the transporter for importing phenylalanine;
b) isolating the resulting bacteria from the growth medium; and
c) suspending the isolated bacteria in a pharmaceutically acceptable carrier.