Patent ID: 11913942
Assignee: IDEXX LABORATORIES, INC.
Field: Measurement (Instruments)
Classification: CPC G  C | IPC C  G

Claim 0:
1. A method for conducting an assay for an analyte in a sample, comprising:
(a) conducting a sample first reaction sequence comprising:
i. forming a sample first reaction mixture by contacting the sample with an anti-analyte antibody, a conjugate comprising the analyte and an enzyme, and a substrate that produces a signal when in contact with the enzyme, and
ii. measuring signal from the sample first reaction mixture;

(b) conducting a sample second reaction sequence comprising,
i. forming a sample second reaction mixture by contacting the sample with the substrate and an anti-analyte antibody,
ii. measuring signal from the sample second reaction mixture;

(c) subtracting an amount of signal from step (b) from an amount of signal from step (a) to provide a net signal,
(d) using the net signal to determine the amount of the analyte in the sample.