Patent ID: 11878080
Assignee: PRESIDENT AND FELLOWS OF HARVARD COLLEGE
Field: Pharmaceuticals (Chemistry)
Classification: CPC A  B | IPC A  B

Claim 0:
1. A population of capsules, wherein each capsule comprises a shell of material that comprises of a self-assembly of one or more proteins, wherein the self-assembly of the one or more proteins is an amyloid assembly of the one or more proteins held together by non-covalent interactions between neighboring protein molecules, wherein the population of capsules is formed by a method comprising:
(i) contacting a flow of a first phase and a flow of a second phase in a channel, thereby generating in the channel a dispersion of discrete regions of the second phase in the first phase, wherein one of the first or second phase is an aqueous phase and the other phase is a water immiscible phase, and the one of the first and second phase comprises one or more proteins suitable for self-assembly, wherein the one or more proteins is provided in monomeric form and is soluble;
(ii) permitting the one or more proteins to self-assemble at the boundary layer of the discrete regions between the first and second phases under denaturing conditions; and
(iii) optionally collecting the outflow from the channel, thereby obtaining a droplet, which contains a capsule,
and wherein the one or more proteins each comprise 50 or more amino acids,
and wherein the droplets have a size distribution having a relative standard deviation (RSD) of at most 10%, and wherein each capsule has an average diameter in the range of 0.5 μm to 100 μm.