Patent ID: 11963982
Assignee: EDITAS MEDICINE, INC.
Field: Pharmaceuticals (Chemistry)
Classification: CPC A  C | IPC A  C

Claim 0:
1. A genome editing system comprising:
a first guide RNA (gRNA) comprising a first targeting domain that differs by no more than 3 nucleotides from SEQ ID NO: 953 or SEQ ID NO:955;
a second gRNA comprising a second targeting domain sequence that differs by no more than 3 nucleotides from SEQ ID NO: 952 or SEQ ID NO:954; and
an RNA-guided nuclease,
wherein (i) the RNA-guided nuclease and the first gRNA and (ii) the RNA-guided nuclease and the second gRNA are configured to associate within a target region in a target nucleic acid and generate a double stranded break (DSB) in the target nucleic acid whereby to create an indel in the target nucleic acid.