Patent ID: 11946037
Assignee: UEA ENTERPRISES, LIMITED
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 0:
1. A method for depleting host nucleic acid in a biological sample, said biological sample having been previously obtained from an animal host, wherein said biological sample comprises animal host cells, comprising:
a) adding to said biological sample a saponin, a DNase, and NaCl and/or KCl, to form a reaction mix, wherein the addition of said NaCl and/or KCl is sufficient to ensure a final concentration of NaCl and/or KCl in the reaction mix of at least 0.2M; and
b) incubating the reaction mix at between 10° C. and 50° C.;
wherein, in said method, lysis of host cells and DNase degradation of host nucleic acid occur at least partially in parallel.