Patent ID: 11945839
Assignee: HOFFMANN-LA ROCHE, INC.
Field: Pharmaceuticals (Chemistry)
Classification: CPC C  A | IPC C

Claim 0:
1. A method for separating a multispecific CrossMab antibody from a light chain (LC) mispaired variant thereof by using a hydrophobic interaction chromatography (HIC) medium, the medium comprising a matrix of particles substituted with ligands,
(i) wherein said particles have an average size of 50 μm or less in diameter and said ligands are butyl groups;
(ii) wherein said particles have an average size of from 35 μm to 60 μm in diameter and said ligands are phenyl groups; or
(iii) wherein said particles have an average size of from 35 μm to 100 μm in diameter and said ligands are polypropylene glycol groups,
wherein the method comprises contacting said multispecific CrossMab antibody and said LC mispaired variant thereof with the HIC medium, and eluting said multispecific CrossMab antibody, thus separating said multispecific CrossMab antibody from said LC mispaired variant thereof.