Patent ID: 11970710
Assignee: NORTH CAROLINA STATE UNIVERSITY
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 0:
1. A composition for genome engineering a target gene in a eukaryotic cell, the composition comprising:
a. a polynucleotide sequence encoding:
i. a Cascade complex comprising a Cse1 polypeptide, a Cse2 polypeptide, a Cas7 polypeptide, a Cas5 polypeptide, and a Cas6e polypeptide,
wherein the polynucleotide sequence encoding the Cse1 polypeptide comprises the polynucleotide sequence of SEQ ID NO: 23, the polynucleotide sequence encoding the Cse2 polypeptide comprises the polynucleotide sequence of SEQ ID NO: 24, the polynucleotide sequence encoding the Cas7 polypeptide comprises the polynucleotide sequence of SEQ ID NO: 25, the polynucleotide sequence encoding the Cas5 polypeptide comprises the polynucleotide sequence of SEQ ID NO: 26, and the polynucleotide sequence encoding the Cas6e polypeptide comprises the polynucleotide sequence of SEQ ID NO: 27; and
ii. a polypeptide domain, wherein the polypeptide domain comprises transcription activation activity, transcription repression activity, transcription release factor activity, histone modification activity, nuclease activity, nucleic acid association activity, methylase activity, or demethylase activity; and

b. a pharmaceutically acceptable carrier.