Patent ID: 11959069
Assignee: PROTEONIC BIOTECHNOLOGY IP B.V.
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 9:
10. A method for amplifying a polynucleotide of interest, the method comprising the steps of
i) providing a recombinant polynucleotide vector comprising the polynucleotide of interest as an insert, and the vector backbone as defined in claim 1;
ii) amplifying the recombinant polynucleotide vector of step i) by transforming a suitable microorganism with the recombinant polynucleotide vector, and culturing said transformed microorganism in a culture medium under conditions suitable for vector amplification to obtain amplified recombinant polynucleotide vector;
iii) isolating the amplified recombinant polynucleotide vector from the culture medium of step ii) to obtain isolated amplified recombinant polynucleotide vector;
iv) contacting the isolated amplified recombinant polynucleotide vector with cleavage means capable of cleaving the first plurality of cleavage sites to produce backbone fragments and the polynucleotide of interest; and optionally,
v) separating the polynucleotide of interest from the backbone fragments of step iv).