Patent ID: 11970741
Assignee: KING FAISAL UNIVERSITY
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 0:
1. A sex-determination method for a dioecious plant, comprising:
obtaining a sample from a dioecious plant; and
determining a presence or absence of a dioecious SRY gene (SEQ ID NO: 2) in the sample,
whereby the presence of SEQ ID NO: 2 in the sample is indicative that the sex of the dioecious plant is male; and
wherein the dioecious plant is a papaya;
wherein determining a presence or absence of SEQ ID NO: 2 in the sample includes:
a) extracting nucleic acid from the dioecious plant sample;
b) contacting under amplification conditions the nucleic acid from the sample with a male-specific dioecious SRY primer pair; and
c) detecting a presence or absence of amplification products,
whereby the presence of amplification products is indicative of the presence of SEQ ID NO: 2 in the sample; and
wherein the male-specific dioecious SRY primer pair is selected from the group consisting of an oligonucleotide including SEQ ID NO: 3 and an oligonucleotide including SEQ ID NO: 8, an oligonucleotide including SEQ ID NO: 4 and an oligonucleotide including SEQ ID NO: 5, and an oligonucleotide including SEQ ID NO: 6 and an oligonucleotide including SEQ ID NO: 7.