Patent ID: 11946033
Assignee: REPLIGEN CORPORATION
Field: Chemical engineering (Chemistry)
Classification: CPC C  B | IPC B  C

Claim 10:
11. A system comprising a housing having a volume of no more than about 15 ml equipped with a micro alternating tangential perfusion device (microATF device) having dimensions suitable for an exchange volume of from 0.5-5 ml comprising:
(a) a hollow cylinder having a proximal end and a distal end, said proximal end being connected in series to (i) a permeate chamber and (ii) a retentate chamber separated from the permeate chamber by a filter element, wherein the proximal end either (1) terminates in or at the permeate chamber, or (2) terminates in or at the retentate chamber, wherein a portion of the proximal end is located within the permeate chamber, and in a case of (2), possesses at least one opening allowing fluid communication between an inside of the hollow cylinder and the permeate chamber;
(b) an inlet, positioned over the retentate chamber, for communication with a source of alternating positive or negative pressure; and
(c) an outlet, positioned in a wall of the permeate chamber, which can be connected to a check valve, which, in turn, can be connected to a hydrophobic fluid vent filter;
and a fluid conduit connecting at least one well of the housing with the proximal end of the microATF device.