Patent ID: 11866749
Assignee: DANMARKS TEKNISKE UNIVERSITET
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 0:
1. A variant of a tryptophan hydroxylase (TPH), the variant comprising
(a) a segment corresponding to residues E147 to T460 of Homo sapiens TPH having the sequence of SEQ ID NO:3,
(b) an N-terminal methionine residue, and
(c) a mutation in at least one residue corresponding to residues N242 and P244 in SEQ ID NO:3, wherein the mutation in the residue corresponding to residue N242 is the amino acid substitution 242I and the mutation in the residue corresponding to P244 is selected from 244C, 244D, 244L and 244Q,
wherein the variant is a variant of a Homo sapiens TPH having the sequence of SEQ ID NO:3, and wherein the segment in the variant has at least about 80% sequence identity to the segment in the native sequence, and
wherein the variant provides for a tryptophan hydroxylation activity which is at least 110% of that of Homo sapiens TPH having the sequence of SEQ ID NO:3.