Patent ID: 11867671
Assignee: HITACHI HIGH-TECH SCIENCE CORPORATION
Field: Measurement (Instruments)
Classification: CPC G | IPC G

Claim 0:
1. A method for separating a basic amino acid from a non-basic amino acid in a mixture of a plurality of amino acids comprising the basic amino acid and the non-basic amino acid, the method comprising:
passing a sample comprising the mixture of the plurality of amino acids together with an eluent, through a separation column packed with a cation exchange resin, thereby the basic amino acid and the non-basic amino acid being eluted separately; and
detecting each of the separately eluted amino acids,
wherein the eluent is only one eluent and has a composition comprising: a polybasic acid, a cation source, and water, and
the eluent has a pH in a range of 5.0 or lower at a temperature of 25° C.,
the composition of the eluent is a same composition throughout the separating of the amino acids by the passing,
the cation source is at least one salt of an alkali metal selected from the group consisting of a lithium (Li) salt and a sodium (Na) salt, and
the sample flows through the separation column that is heated by applying a temperature gradient including a temperature region of 100° C. or higher.