Patent ID: 11959145
Assignee: HANNELORE BREITENBACH-KOLLER
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 3:
4. A kit for identifying a compound that ameliorates or reverts a defective or undesired translation of a target gene bearing a premature termination codon (PTC), comprising
a) a recombinant eukaryotic cell comprising a ribosomal protein deletion or other mutation that causes said defective or undesired translation of a PTC-bearing target gene wherein said ribosomal protein is selected from the group consisting of rPL19A, rPL25, and rPL10,
b) a receptor plasmid comprising a PTC-bearing target gene selected from the group consisting of a LAMB3 gene and a CYH2 gene that is inserted in frame with and upstream of a first reporter group and a control plasmid harboring a second reporter group,
c) instructions for practicing a method of identifying a compound that ameliorates or reverts a defective translation of the PTC-bearing target gene.