Patent ID: 11884952
Assignee: ZHEJIANG UNIVERSITY
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 2:
3. The method according to claim 2, wherein the agarslantculture-medium in the step (1) is a R5 medium, each liter of medium which contains 103 g of sucrose, 10 g of glucose, 5 g of yeast extract, 0.1 g of casein hydrolysate, 0.25 g of K2SO4, 10.12 g of MgCl2·6H2O, 2 mL of a trace element solution, 5.73 g of TES buffer, and 0.3 g of proline, which are diluted by water to a volume of 1 L, and 22 g of agar is added to a solid medium;
in the step (2), the seed broth is a Trypticase Soy Broth medium: TSB 2%, PEG 6000 5%, which are diluted by water to a volume of 1 L, wherein the percentages are percentages by mass; and
the fermentation medium in the step (2) is a yeast malt medium: 0.3% yeast extract, 0.3% malt extract, 0.5% tryptone, and 4% glucose, which are diluted to a volume of 1 L.