Patent ID: 11873504
Assignee: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 0:
1. One or more nucleic acid molecules encoding one or more of:
(a) a CasX guide RNA comprising an activator RNA and a targeter RNA, wherein the targeter RNA is heterologous to the activator RNA,
wherein one nucleic molecule comprises a first nucleotide sequence encoding the activator RNA and a second nucleotide sequence encoding the targeter RNA, wherein the first nucleotide sequence is heterologous to the second nucleotide sequence, or
wherein a first nucleic molecule comprises a nucleotide sequence encoding the activator RNA and a second nucleic molecule comprises a nucleotide sequence encoding the targeter RNA, wherein the first nucleic molecule is heterologous to the second nucleic molecule,
and
wherein the targeter RNA comprises (i) a guide sequence that hybridizes to a target nucleic acid, and (ii) a duplex-forming segment and the activator RNA hybridizes with the duplex-forming segment to form a double stranded RNA (dsRNA) duplex that binds a CasX polypeptide and guides the bound CasX polypeptide to the target nucleic acid;
(b) a CasX polypeptide, wherein the one nucleic acid molecule comprises a promoter operably linked to and heterologous to the sequence encoding the CasX polypeptide; and
(c) a CasX fusion polypeptide comprising a CasX polypeptide fused to a heterologous polypeptide, wherein the one nucleic acid molecule comprises a nucleotide sequence encoding the CasX fusion polypeptide.