Patent ID: 11891660
Assignee: SINGULAR GENOMICS SYSTEMS, INC.
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 19:
20. A method of forming a template polynucleotide on a substrate, said method comprising:
a) ligating a first end of a double-stranded nucleic acid to a first adapter, wherein said first adapter is attached to said substrate, and wherein said first adapter is a Y-adapter comprising a first primer binding sequence and a second primer binding sequence;
b) ligating a second adapter to a second end of the double-stranded nucleic acid, wherein the second adapter is a hairpin adapter and comprises a third primer binding sequence, thereby forming said template polynucleotide on said substrate;
wherein the first primer binding sequence, the second primer binding sequence, and the third primer binding sequence are different.