Patent ID: 11884963
Assignee: TAKARA BIO USA, INC.
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 12:
13. A method of producing a composition selectively enriched for one or more target nucleic acids, the method comprising:
obtaining a composition comprising both target nucleic acids and non-target nucleic acids;
contacting the composition with one or more cleavage-deficient nucleases that lack nuclease activity and one or more guide nucleic acids that guide the cleavage-deficient nucleases to the one or more target nucleic acids to produce a contacted composition, wherein the contacted composition comprises the target nucleic acids, the non-target nucleic acids, the one or more cleavage-deficient nucleases, and the one or more guide nucleic acids, and the one or more guide nucleic acids specifically hybridize with the one or more target nucleic acids to form a complex; and
separating the complex from the contacted composition, thereby producing a composition selectively enriched with the one or more target nucleic acids.