Patent ID: 11859245
Assignee: INSTITUTE OF ZOOLOGY, CHINESE ACADEMY OF SCIENCES
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 5:
6. A method for detecting telomerase activity of a biological sample using the primer set of claim 1, the method comprising:
1) lysing the biological sample with a 3-((3-cholamidopropyl)dimethylammonio)propanesulfonate (CHAPS) lysis buffer to obtain a cell lysate solution;
2) performing a telomerase extension in the cell lysate solution in the presence of the upstream primer of the first primer set or the second primer set of the primer set to obtain a telomerase template; and
3) conducting a qPCR amplification with the telomerase template in the presence of a corresponding downstream primer of the upstream primer of the first primer set or the second primer set used in the telomerase extension in 2).