Patent ID: 11911457
Assignee: BHARAT BIOTECH INTERNATIONAL LTD.
Field: Pharmaceuticals (Chemistry)
Classification: CPC A  C  Y | IPC A  C

Claim 14:
15. A method for preparing a dose reduced inactivated Polio vaccine containing Sabin polioviruses, said method comprising the steps of:
a. producing polioviruses in Vero cells;
b. purification of the polioviruses by two step chromatography;
c. stabilization of purified polioviruses by addition of M-199 medium containing glycine and tween 80;
d. inactivation of the polio viruses by using formaldehyde 0.025% at 37° C. for 13 days in presence of phosphate buffer with saline at a concentration between 2 mM to 10 mM or sodium acetate to reduce aggregation of the poliovirus particles thereby reducing the poliovirus particles losses post inactivation by greater than 90% as compared to other buffers; and
e. formulating the said polio vaccine, wherein the two step chromatography is carried out by first running size exclusion chromatography followed by ion exchange chromatography.