Patent ID: 11874247
Assignee: MCMASTER UNIVERSITY
Field: Measurement (Instruments)
Classification: CPC G | IPC G

Claim 14:
15. A method of detecting a target analyte in a sample, the method comprising:
a) contacting the sample with the biosensor of claim 1 under conditions for
i) binding the target analyte to the first capture probe and the second capture probe, and binding the target analyte to the one or more reporter moieties,
ii) binding the target analyte to the one or more reporter moieties, or
iii) binding the target analyte to the first capture probe and the second capture probe,

b) measuring a detection signal from each of the first photoelectrode and the second photoelectrode,
whereby
in a) i) upon binding the target analyte to the one or more reporter moieties and to the first capture probe, a detection signal is generated by transfer of electrons between the detectable label and the photoactive material on the first photoelectrode, and upon binding the target analyte to the one or more reporter moieties and the second capture probe, a detection signal is generated by transfer of electrons between the detectable label and the photoactive material on the second photoelectrode, wherein the first capture probe is capable of binding to a portion of the target analyte by complementarity, the second capture probe is capable of binding to a portion the target analyte by complementarity, and the one or more reporter moieties are capable of binding to a portion of the target analyte by complementarity, and wherein the portion of the target analyte that can bind to the first capture probe or the second capture probe is a different portion than can bind to the one or more reporter moieties, or
in a) ii) upon binding the target analyte to the one or more reporter moieties, the one or more reporter moieties are activated and a fragment of the one or more reporter moieties comprising the detectable label is released, and the released fragment then binds to the first capture probe and the second capture probe, or
in a) iii) upon binding the target analyte to the first capture probe comprising a first reporter moiety, the first reporter moiety comprising the detectable label is retained in the first capture probe, and upon binding of the target analyte to the second capture probe comprising a second reporter moiety, the second reporter moiety comprising the detectable label is released from the second capture probe,
wherein a higher intensity of the detection signal from the first photoelectrode as compared to the second photoelectrode in the presence of the sample, or a higher increase in the intensity of the detection signal from the first photoelectrode as compared to the second photoelectrode in the presence of the sample as compared to in the absence of the sample, is indicative of the presence of the target analyte.