Patent ID: 11919925
Assignee: ALEXION PHARMACEUTICALS, INC.
Field: Chemical engineering (Chemistry)
Classification: CPC C  B | IPC A  B  C

Claim 0:
1. A method of performing viral filtration, the method comprising the steps of:
(a) adjusting the pH of a fluid comprising a recombinant antibody to between about 5.0 and about 6.7, and adding a stabilizing agent to the fluid in an amount sufficient to yield a final concentration of between about 0.1 mM and about 25 mM stabilizing agent in the fluid;
(b) flowing the fluid through a pre-filter, wherein the pre-filter comprises a polyamide membrane; and
(c) immediately following step (b), flowing the fluid through a virus filter to produce a filtrate comprising the recombinant antibody,
wherein:
(i) the stabilizing agent is selected from the group consisting of arginine, alanine, aspartic acid, glutamic acid, leucine, lysine, histidine, glycine, sucrose, trehalose, mannitol, and sorbitol; and
(ii) (ii) the recombinant antibody comprises:
a heavy chain variable region comprising:
a CDR1 comprising a sequence of SEQ ID NO: 1;
a CDR2 comprising a sequence of SEQ ID NO: 2; and
a CDR3 comprising a sequence of SEQ ID NO: 3; and

a light chain variable region comprising
a CDR1 comprising a sequence of SEQ ID NO: 6;
a CDR2 comprising a sequence of SEQ ID NO: 7; and
a CDR3 comprising a sequence of SEQ ID NO: 8.