Patent ID: 11866766
Assignee: BIO-RAD LABORATORIES, INC.
Field: Biotechnology (Chemistry)
Classification: CPC C  B | IPC C

Claim 16:
17. A method comprising:
transferring a biological sample comprising target nucleic acid material and a process reagent into a fluid pathway over an array of wells defined at a surface plane of a substrate, wherein the process reagent comprises water, wherein the array of wells comprises from 10,000 wells through 250,000 wells wherein each well in the array of wells extends vertically into a planar surface of the substrate, and wherein the fluid pathway directs flow into the array of wells, linearly from a first subset of wells of the array of wells at a first end of the substrate, to a second subset of wells of the array of wells at a second end of the substrate;
achieving a state of specific capture for the array of wells, wherein a well of the array of wells in the state of specific capture has a target of a set of targets of the target nucleic acid material;
distributing a liquid immiscible with water into the fluid pathway under positive pressure, thereby isolating each well of the array of wells from adjacent wells of the array of wells, at the surface plane and mitigating leakage of said target nucleic acid material of a first well to a second well of the array of wells;
modulating temperature of contents within the array of wells; and
illuminating and optically imaging contents of the array of wells.