Patent ID: 11859245
Assignee: INSTITUTE OF ZOOLOGY, CHINESE ACADEMY OF SCIENCES
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 9:
10. The method of claim 9, wherein the reaction solution is treated under an extension reaction program and a PCR reaction program consecutively; the extension reaction program comprises incubation at 25° C. for 25 min, and denaturation at 94° C. for 2 min; the PCR reaction program comprises: a) denaturation at 94° C. for 30 s; b) annealing at 57-60° C. for 30 s; c) extension at 72° C. for 30 s; d. repeating operations b)-c) 45-50 times; and e) extension at 72° C. for 10 min.