Patent ID: 11913055
Assignee: FUDAN UNIVERSITY
Field: Biotechnology (Chemistry)
Classification: CPC C  Y | IPC C

Claim 1:
2. The method of claim 1, wherein in step (1), the inert solid medium is a composite material of polyvinyl alcohol and polyethylene glycol; and
the step of co-immobilizing a carbonyl reductase and an isopropanol dehydrogenase onto an inert solid medium to prepare the co-immobilized catalyst comprises:
(a) preparing an aqueous solution of the polyvinyl alcohol and the polyethylene glycol; heating the aqueous solution until the aqueous solution becomes clear; and cooling the aqueous solution to 50° C. or less to obtain a first solution;
(b) adding a crude carbonyl reductase solution and a crude isopropanol dehydrogenase solution into the first solution followed by uniform mixing to obtain a second solution; and
(c) dropwise adding the second solution onto a polyethylene film; drying the polyethylene film at 35-40° C. for 0.5-1 hour to obtain the co-immobilized catalyst; and storing the co-immobilized catalyst at 4° C. for later use;
wherein
a weight ratio of the polyvinyl alcohol to the polyethylene glycol is 5:1-3;
the crude carbonyl reductase solution and the crude isopropanol dehydrogenase solution both have an initial concentration of 10%-30% (w/v); and
in step (b), a volume ratio of the crude carbonyl reductase solution to the crude isopropanol dehydrogenase solution to the first solution in the second solution is 2:1:5-10.