Patent ID: 11905554
Assignee: HAINAN UNIVERSITY
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 0:
1. A PCR based whole genome sequencing process comprising:
preparing DNA of a sample to be tested;
providing a sequencing primer set, conducting a first PCR amplification on the DNA of the sample to be tested by using the universal upstream primer and the universal downstream primer to obtain a first amplification product, and conducting a second PCR amplification on the DNA of the sample to be tested by using the universal upstream primer and the downstream primer with promoter to obtain a second amplification product;
mixing the first amplification product and the second amplification product, and carrying out sequencing analysis;
wherein the sequencing primer set comprising a universal upstream primer, a universal downstream primer and a downstream primer with promoter;
wherein the universal upstream primer has a sequence of: 5′-TbarcodeCAAAXXXXNNN-3′; the universal downstream primer has a sequence of: 5′-GACTGCGTACGZZZZNNN-3′ that is SEQ ID No. 9; the downstream primer with promoter has a sequence of 5′-GACTGCGTACYYNCTATA-3′ that is SEQ ID No. 7;
wherein the “XXXX” in the universal upstream primer has 4 bases, and each base of the “XXXX” is selected from the group consistong of A, T, C and G: the “ZZZZ” in the universal downstream primer has 4 bases, and each base of the “ZZZZ” is selected from the group consisting of A, T, C and G; the “barcode” in the universal upstream primer has 4-6 bases, and each base of the “barcode” is selected from the group consisting of A, T, C and G, and the “N” is selected from the group consisting of A, T, C and G, the “Y” is a base C or T.