Patent ID: 11897831
Assignee: HANGZHOU TONGZHOU BIOTECHNOLOGY CO., LTD.
Field: Measurement (Instruments)
Classification: CPC C  G | IPC C  G

Claim 1:
2. A method for preparing the pregabalin artificial hapten of claim 1,
including the following steps:
(1) dissolving pregabalin in methanol, adding concentrated sulfuric acid, stirring for reaction while refluxing at 68° C. for 24 hours; neutralizing the reaction product in sodium hydrogen carbonate solid, evaporating to dryness, and extracting with tetrahydrofuran to obtain a yellow oily product A; and
(2) mixing the yellow oily product A with maleic anhydride in the molar ratio of 1:2 to 3 in dichloromethane, stirring for reaction at room temperature for 20 hours; after the reaction, washing, drying, filtering and rotationally drying the product, and then separating from a thin layer chromatography to get a light yellow oily product which is the pregabalin artificial hapten I.