Patent ID: 11912978
Assignee: THE TRUSTEES OF THE UNIVERSITY OF PENNSYLVANIA
Field: Pharmaceuticals (Chemistry)
Classification: CPC A  C  B | IPC A  B  C

Claim 12:
13. A method for processing paramagnetic particle-bound cells, comprising:
flowing a cell suspension comprising paramagnetic particle-bound cells and unbound cells through a flow chamber of a flow-through magnetic separation/debeading module; and
flowing fluid through a plurality of fluid injection ports arranged along the flow-through magnetic separation/debeading module, the plurality of fluid injection ports being positioned between a first end and a second end of the flow-through magnetic separation/debeading module, the fluid flowing through the plurality of fluid injection ports dislodging cells from a membrane positioned adjacent to the plurality of fluid injection ports, the dislodged cells passing further through the flow chamber;
wherein the magnetic separation/debeading module establishes a magnetic force within the flow chamber and a flow of the cell suspension through the flow chamber to both separate the paramagnetic particle-bound cells from the unbound cells and to debead a portion of the paramagnetic particle-bound cells.