Patent ID: 11893779
Assignee: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA
Field: Measurement (Instruments)
Classification: CPC G | IPC G

Claim 18:
19. A method of detecting motile objects in a sample comprising:
obtaining a plurality of time-varying holographic speckle pattern image sequences of the sample using a moveable scanning head containing one or more coherent light sources and corresponding image sensor(s) associated with the one or more coherent light sources wherein no lens is present between the one or more coherent light sources and the corresponding image sensor(s); and
processing the plurality of time-varying holographic speckle pattern image sequences with a computing device configured to receive the time-varying holographic speckle pattern image sequences obtained by the image sensor(s), the computing device comprising computational motion analysis software configured to generate a three-dimensional (3D) contrast map of the motile objects within the one or more optically transparent sample holders that back-propagates the time-varying holographic speckle pattern image sequences followed by differential analysis, image filtering, and thresholdinq to find connected regions, the computing device further comprising a deep learning-based classifier software to identify motile objects in the three-dimensional (3D) contrast map.