Patent ID: 11859224
Assignee: TATE & LYLE SOLUTIONS USA LLC
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 10:
11. A method of manufacturing a product comprising the steps of:
(a) producing allulose by:
(i) providing a vector comprising a nucleic acid molecule having a polynucleotide sequence encoding a protein having allulose 3-epimerase activity;
(ii) synthesizing the protein having allulose 3-epimerase activity from the vector;
(iii) contacting fructose with the protein having allulose 3-epimerase activity and maintaining the fructose and the protein under conditions to permit the conversion of fructose to allulose;
wherein the protein having allulose 3-epimerase activity comprises an amino acid sequence having at least 90% sequence identity to the amino acid sequence of SEQ ID NO: 6; and

(b) using the allulose produced in step (a) to manufacture the product.