Patent ID: 11866775
Assignee: BROOKHAVEN SCIENCE ASSOCIATES, LLC
Field: Biotechnology (Chemistry)
Classification: CPC C  B | IPC B  C

Claim 0:
1. A method for amplifying a molecular signal of an Input-Oligonucleotide, the method comprising
a) providing a plurality of Particle-A protected by oligonucleotide origami, wherein Particle-A is functionalized with a plurality of Oligonucleotide-1, wherein a sequence in Oligonucleotide 1 complementary to a sequence in a plurality of Oligonucleotide 2 hybridize to form Duplex 1-2, and sequence in Oligonucleotide 2 complementary to a sequence in oligonucleotide origami hybridize;
b) providing a plurality of Particle-B, wherein Particle-B is functionalized with a plurality of Oligonucleotide-3, wherein a sequence in Oligonucleotide-3 complementary to a sequence in a plurality of Stored-Input-Oligonucleotide hybridize to form Duplex 3-Y; and
c) mixing the Input-Oligonucleotide, plurality of origami protected Particle-A and plurality of Particle-B, in any order, wherein a cascade reaction occurs so that:
i) sequence in the Input-Oligonucleotide complementary to sequence in Oligonucleotide-2 hybridize to form Duplex Y-2, thereby removing Oligonucleotide-2 and the oligonucleotide origami, and exposing Oligonucleotide-1 on Particle A,
ii) sequence in Oligonucleotide-1 complementary to sequence in Oligonucleotide-3 hybridize to form Duplex 1-3, thereby releasing the Stored-Input-Oligonucleotide hybridized to the sequence in Oligonucleotide-3,
iii) sequence in the Stored-Input Oligonucleotide released in ii) complementary to sequence in Oligonucleotide-2 hybridize to form Duplex Y-2, thereby removing Oligonucleotide-2 and the oligonucleotide origami, and exposing Oligonucleotide-1 on Particle A to hybridize to complementary sequence in Oligonucleotide-3 to form Duplex 1-3, thereby releasing the Stored-Input-Oligonucleotide hybridized to the sequence in Oligonucleotide-3, and,
iv) where (iii) reoccurs until the cascade reaction terminates, when Particle A are fully deprotected.