Patent ID: 11913951
Assignee: ALEXION PHARMACEUTICALS, INC.
Field: Measurement (Instruments)
Classification: CPC G | IPC G

Claim 18:
19. A method of detecting C3 in a biological sample, the method comprising:
(a) incubating the biological sample with a first and second antibody that bind to human C3, wherein the first antibody binds to an epitope on C3a and the second antibody binds to an epitope on C3b, and wherein the antibodies are FRET pairs; and
(b) performing fluorescence resonance energy transfer (FRET) to detect the FRET signal, thereby detecting C3 in the biological sample;
(c) quantifying the amount of C3 in the biological sample by comparing the FRET signal to a standard curve; and
(d) repeating steps (a), (b), and (c) sequentially over a time course,, wherein the first antibody comprises heavy chain CDR1, CDR2, and CDR3 amino acid sequences as set forth in SEQ ID NOs: 1, 2, and 3, respectively, and light chain CDR1, CDR2, and CDR3 amino acid sequences as set forth in SEQ ID NOs: 4, 5, and 6, respectively.