Patent ID: 11859230
Assignee: THE REGENTS OF THE UNIVERSITY OF MICHIGAN
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 0:
1. A method of producing γ-polyglutamic acid (PGA), comprising:
a) contacting an Aspergillus niger, Yarrowia lipolytica, or Candida oleophila fungus with a first feedstock in a bioreactor;
b) fermenting said fungus to produce a culture broth comprising citric acid in the presence of a nitrogen source at a concentration of 0.1-50 g total N/L and a phosphorous source at a concentration of 0.1-25 g total P/L;
c) contacting said bioreactor comprising said culture broth comprising citric acid from step b), without purification, with a Bacillus sp., wherein said Bacillus sp. is Bacillus subtilis or Bacillus licheniformis and a second feedstock; and
d) fermenting said Bacillus sp. to generate PGA in the presence of a nitrogen source at a concentration of 0.1-50 g total N/L and a phosphorous source at a concentration of 0.1-25 g total P/L.