Patent ID: 11946081
Assignee: DANISCO US INC.
Field: Basic materials chemistry (Chemistry)
Classification: CPC C | IPC C

Claim 1:
2. The subtilisin variant of claim 1, wherein the variant has combinations of mutations selected from the group consisting of:
S039E-S099R-S126A-D127E-F128G-M211L, 5039E-S099R-S126A-D127E-F128G-N242D, 5039E-S099R-S126A-D127E-F128G-N253D, 5039E-S099R-S126A-D127E-F128G-Q200L, 5039E-S099R-S126A-D127E-F128G-Q256E, 5039E-S099R-S126A-D127E-F128G-S255W, 5039E-5099R-S126A-D127E-F128G-V199I, S039E-5099R-S126A-D127E-F128G-Y203W, 5039E-5099R-T1140-S126A-D127E-F128G, 5039E-T056Y-N074D-S099R-S126A-D127E-F128G, 5039E-T056Y-S099R-S126A-D127E-F128G, 5039E-T056Y-S099R-S126A-D127E-F128G-M211L, and T009E-5039E-5099R-S126A-D127E-F128G, numbered by correspondence with the amino acid sequence of SEQ ID NO:1.