Patent ID: 11892449
Assignee: NEW YORK UNIVERSITY
Field: Measurement (Instruments)
Classification: CPC G  A  C | IPC A  C  G

Claim 0:
1. A protein comprising one or more X1 blocks, X2 blocks, and X3 blocks, wherein the X1 block is a coiled-coil domain, the X2 block is a linker, and the X3 block is a binding domain for a target protein, wherein the X1, X2, and X3 blocks are oriented from the N to C-terminus as (X1)a-(X2)b-(X3)c or (X3)c-(X2)b-(X1)a, wherein a, b, and c are the number of repeat units for the blocks, wherein a, b, and c are each independently 1 to 50,
wherein X3 is IEEQAKTFLDKFNGEELLRALDQVN (SEQ ID NO:39) or a sequence having at least 85% identity thereto and X1 comprises the following sequence:, (SEQ ID NO: 3)

MRGSHHHHHHGSACELAATATATATATATAACGDLAPQMLRELQETNAAL

QDVRELLRQQVKEITFLKNTLLEIWKAAAK;

 

(SEQ ID NO: 4)

MRGSHHHHHHGSASELAATATATATATATAASGDLAPQMLRELQETNAAL

QDVRELLRQQVKEITFLKNTLLEIWKAAAK;

 

(SEQ ID NO: 5)

MRGSHHHHHHGSACELAATATATATATATAACGDLAPQMLRELQETNAAL

QDVRELLRQQVKEITFLKNTLLEIWK;

 

(SEQ ID NO: 6)

MRGSHHHHHHGSASELAATATATATATATAASGDLAPQMLRELQETNAAL

QDVRELLRQQVKEITFLKNTLLEIWK;

 

(SEQ ID NO: 172)

MRGSHHHHHHGSACELAATATATATATATAACGDLAPQMLRELQETNAAL

QDVRELLRQQVKEITFLKNT,

 

(SEQ ID NO: 173)

MRGSHHHHHHGSASELAATATATATATATAASGDLAPQMLRELQETNAAL

QDVRELLRQQVKEITFLKNT,

 

(SEQ ID NO: 8)

GSACELAATATATATATATAACGDLAPQMLRELQETNAALQDVRELLRQQ

VKEITFLKNTLLEIWKAAAK;

 

(SEQ ID NO: 9)

GSASELAATATATATATATAASGDLAPQMLRELQETNAALQDVRELLRQQ

VKEITFLKNTLLEIWKAAAK;

 

(SEQ ID NO: 10)

GSACELAATATATATATATAACGDLAPQMLRELQETNAALQDVRELLRQQ

VKEITFLKNTLLEIWK;

 

(SEQ ID NO: 11)

GSASELAATATATATATATAASGDLAPQMLRELQETNAALQDVRELLRQQ

VKEITFLKNTLLEIWK;

 

(SEQ ID NO: 174)

MRGSHHHHHHGSACELAATATATATATATAACGDLAPQMLRELQETNAAL

QDVRELLRQQVKEITFLKNTLLEIWK,

 

(SEQ ID NO: 175)

MRGSHHHHHHGSASELAATATATATATATAASGDLAPQMLRELQETNAAL

QDVRELLRQQVKEITFLKNTLLEIWK,

 

(SEQ ID NO: 12)

DLAPQMLRELQETNAALQDVRELLRQQVKEITFLKNTLLEIWKAAK,

 

(SEQ ID NO: 13)

DLAPQMLRELQETNAALQDVRELLRQQVKEITFLKNTLLEIWK,

 

(SEQ ID NO: 14)

DLAPQMLRELQETNAALQDVRELLRQQVKEITFLKNT,

or
a sequence having at least 85% identity with any one of SEQ ID NOs:3-6, 8-14, and 172-175.