Patent ID: 11965215
Assignee: THE BOARD OF TRUSTEES OF THE LELAND STANFORD JUNIOR UNIVERSITY
Field: Medical technology (Instruments)
Classification: CPC C  G | IPC C  G

Claim 0:
1. A method comprising:
(a) obtaining, by a computer system, sequencing data for at least 1,000 cell-free DNA molecules from a first sample of a human subject;
(b) processing, by the computer system, the sequencing data of (a) to identify one or more cell-free DNA molecules of the at least 1,000 cell-free DNA molecules as phased variant-containing cell-free DNA molecules, wherein identifying the one or more cell-free DNA molecules as phased variant-containing cell-free DNA molecules comprises aligning reads corresponding to each of the at least 1,000 cell-free DNA molecules to a reference human genomic sequence of at least 10 kb in length, wherein each of the one or more cell-free nucleic acid molecules identified as phased variant-containing cell-free DNA molecules comprises a plurality of phased variants relative to a sequence derived from a second sample of the same subject, wherein at least 10% of the one or more cell-free nucleic acid molecules identified as phased variant-containing cell-free DNA molecules comprises a first phased variant of the plurality of phased variants and a second phased variant of the plurality of phased variants that are separated by at least one nucleotide; and
(c) analyzing, by the computer system, the identified one or more cell-free nucleic acid molecules identified as phased variant-containing cell-free DNA molecules to determine a condition of the subject, wherein the condition comprises a cancer which is not a hematological malignancy.