Patent ID: 11944964
Assignee: WASHINGTON UNIVERSITY
Field: Chemical engineering (Chemistry)
Classification: CPC B | IPC B

Claim 0:
1. A micro-bioelectrochemical cell (μ-BEC) device comprising a plurality of chambers, each chamber enclosing a volume ranging from about 1 μL to about 1.6 μL, the device comprising:
a support layer, comprising a support contact surface, wherein at least a portion of the contact surface is coated with a working electrode layer;
a microfluidics layer comprising opposed first and second surfaces and containing a plurality of wells formed therethrough;
an electrical layer comprising an electrical contact layer, a plurality of counter electrodes positioned on the electrical contact layer and a plurality of reference electrodes positioned on the electrical contact layer;
wherein:
the first surface is bonded to the support contact surface and the second surface is bonded to the electrical contact layer in alignment to form the plurality of chambers, each chamber comprising one well sealed between one portion of the working electrode layer and one portion of the electrical layer containing one counter electrode and one reference electrode the one portion of the working electrode layer, the one counter electrode, and the one reference electrode are in electrical contact with the chamber
at least one portion of chambers from the plurality of chambers is microfluidically connected; and
the support layer and the working electrode layer are transparent to provide for simultaneous electrochemical measurements and imaging of the volumes enclosed within the plurality of chambers.