Patent ID: 11862296
Assignee: NATIONAL KAOHSIUNG UNIVERSITY OF SCIENCE AND TECHNOLOGY
Field: Computer technology (Electrical engineering)
Classification: CPC G | IPC G

Claim 7:
8. The method for designing the multi-objective primer pair as claimed in claim 1, wherein the at least two objectives include the seventh objective calculating the quantity of bindings generated by the nucleotides of two forward primers of the plurality of forward primers and a plurality of reverse primers provided simultaneously when a polymerase chain reaction is carried out to obtain a first quantity, wherein if the first quantity is not greater than an optimal forward primer self-binding quantity, a forward primer self-binding quantity is equal to 0;
if the first quantity is greater than the optimal forward primer self-binding quantity, the forward primer self-binding quantity is equal to a difference between the first quantity and the optimal forward primer self-binding quantity; and
calculating the quantity of bindings generated by the nucleotides of two reverse primers to obtain a second quantity, wherein if the second quantity is greater than an optimal reverse primer self-binding quantity, the reverse primer self-binding quantity is equal to a difference between the second quantity and the optimal reverse primer self-binding quantity, and calculating the quantity of bindings generated by the nucleotides of one of the plurality of forward primers and one of the plurality of reverse primers to obtain a third quantity, wherein if the third quantity is not greater than an optimal cross-binding quantity, a primer cross-binding quantity is equal to 0;
if the third quantity is greater than the optimal cross-binding quantity, the primer cross-binding quantity is equal to a difference between the third quantity and the optimal cross-binding quantity, and the seventh objective is equal to a sum of the forward primer self-binding quantity, the reverse primer self-binding quantity and the primer cross-binding quantity.