Patent ID: 11920120
Assignee: GENZYME CORPORATION
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 0:
1. A method of decreasing contamination of a sterilized biological manufacturing system, the method comprising:
(a) providing a sterilized biological manufacturing system and an isolation vessel, wherein:
the sterilized biological manufacturing system comprises a liquid comprising a recombinant therapeutic protein;
the isolation vessel comprises a headspace of ozone and a liquid; and
the sterilized biological manufacturing system comprises a fluid outlet in fluid communication with a fluid inlet of the isolation vessel; and
(b) continuously flowing a first volume of the liquid out of the fluid outlet of the sterilized biological manufacturing system, into the fluid inlet of the isolation vessel, and through the headspace of the isolation vessel,
wherein:
the sterilized biological manufacturing system is a bioreactor, a chromatography system, a microfiltration (MF) system, or an ultrafiltration/diafiltration (UF/DF) system; and
ozone is sparged into the isolation vessel or introduced directly into the headspace of the isolation vessel; and
the concentration of ozone is controlled within the headspace of the isolation vessel to provide a sterilizing atmosphere.