Patent ID: 11944688
Assignee: SUTURA THERAPEUTICS LTD
Field: Pharmaceuticals (Chemistry)
Classification: CPC A  C | IPC A  C

Claim 22:
23. A method making a molecule comprising the step of conjugating:
i) a biologically active siRNA or antisense oligonucleotide moiety, comprising a phosphordiamidate morpholino oligonucleotide to,
ii) a peptide moiety comprising a stapled peptide (StaP) or a stitched peptide (StiP), wherein the StaP or StiP is a stabilized peptide which has a conformation comprising at least one alpha helix by olefin cross linking comprising in the StaP an olefin cross link between two unnatural amino acids of the peptide at positions i, i+4, and/or i, i+7 and in the StiP at least two olefin cross links between at least three unnatural amino acids of the peptide at positions i, i+4, and i+11, and the StaP or the StiP can penetrate a cell membrane,
wherein the StaP or StiP has the amino acid sequence comprising SEQ ID NO: 27, 28, 33, 41, 44, 57, 88, 89, 90, 91, 92, 93, 94, 109, 140, 141, 159, 160, 162, 163, 164, or 165,
wherein the biologically active siRNA or antisense oligonucleotide moiety is covalently linked directly to the StiP or the StaP via a bi-functional linker moiety (BFL) to the StiP or the StaP,
wherein the molecule can penetrate a cell membrane, and has biological activity of the siRNA or antisense oligonucleotide moiety.