Patent ID: 11931361
Assignee: HER MAJESTY THE QUEEN IN RIGHT OF CANADA, AS REPRESENTED BY THE MINISTER OF AGRICULTURE AND AGRI-FOOD
Field: Pharmaceuticals (Chemistry)
Classification: CPC A  C | IPC A  C

Claim 12:
13. A method for generating recombinant orthopox virus having altered host permissiveness comprising:
in an orthopoxvirus, replacing at least one native amino acid within domain C of the orthopoxvirus K3 protein as set forth in SEQ ID No: 12 with a non-native amino acid, thereby generating a recombinant orthopox virus;
transfecting a panel of possible host cell types with the recombinant orthopoxvirus; and
detecting recombinant orthopoxrus growth within each respective one possible host cell type, wherein recombinant orthopoxvirus growth within a respective one possible host cell type indicates that the respective one host cell type is a permissive host cell type for the recombinant orthopoxvirus.