Patent ID: 11911712
Assignee: FUJIFILM DIOSYNTH BIOTECHNOLOGIES UK LIMITED
Field: Chemical engineering (Chemistry)
Classification: CPC B  G | IPC B  G

Claim 0:
1. A method for preparing an aqueous solution of a defined pH comprising an acid, a base and one or more additives, wherein the method comprises the steps of:
a) calculating theoretical concentrations of acid and base for each of a plurality of solutions having a theoretical pH corresponding to the defined pH, and each of the plurality of solutions having a different additive concentration within a range of different additive concentrations, wherein said calculating is carried out using the Henderson-Hasselbach equation in combination with the Debye Huckel theory;
b) preparing the plurality of solutions and measuring an actual pH for each solution having a different additive concentration within the range of different additive concentrations;
c) for each solution, calculating a value for delta pH, ΔpH, being the difference between the theoretical pH and the actual pH;
d) generating a mathematical model describing a relationship of ΔpH with additive concentration;
e) selecting the defined pH and additive concentration for the aqueous solution to be prepared;
f) using the mathematical model generated in step d) to calculate ΔpH for the selected defined pH and additive concentration;
g) calculating a ΔpH-corrected pH by summing the defined pH and ΔpH;
h) using the ΔpH-corrected pH to calculate concentrations of acid and base for the preparation of the aqueous solution including the additive by using the Henderson-Hasselbach equation in combination with the Debye Huckel theory; and
i) preparing the aqueous solution including the one or more additives using the concentrations of acid and base calculated in step h).