Patent ID: 11920194
Assignee: BIONTECH SE
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 4:
5. A method for screening for a genotype for loss of antigen presentation via MHC class I, by identifying a chromosomal deletion and inversion event in a chromosome 15 resulting in the loss of the beta-2-microglobulin (B2M) locus comprising: obtaining a sample containing nucleic acid from a subject, hybridizing to the nucleic acid a group of primers, amplifying the hybridized group of primers, and detecting the presence or absence of an amplification product, wherein detection of the amplification product indicates the presence of a chromosomal deletion and inversion event in a chromosome 15 resulting in the loss of the beta-2-microglobulin (B2M) locus that is predictive of a genotype for loss of antigen presentation via MHC class I;
wherein the group of primers is selected from: (a) the group of primers comprising the sequences set forth in SEQ ID NO: 15 and SEQ ID NO: 16; (b) the group of primers comprising the sequences set forth in SEQ ID NO: 18 and SEQ ID NO: 19; and (c) the group of primers comprising the sequences set forth in SEQ ID NO: 22 and SEQ ID NO: 23.