Patent ID: 11959897
Assignee: REGENERON PHARMACEUTICALS, INC.
Field: Measurement (Instruments)
Classification: CPC G  H | IPC G  H

Claim 0:
1. A method of characterizing a sample, comprising:
providing the sample to a liquid chromatography system capable of sample separation to generate separated sample components;
analyzing separated sample components by multiplexed targeted selected ion monitoring (SIM) to select a plurality of precursor ions; and
performing iterative mass spectral data-dependent acquisition (DDA) on the plurality of precursor ions,
wherein preforming iterative mass spectral data-dependent acquisition comprises utilizing an iterative inclusion list and an iterative exclusion list, wherein the iterative inclusion list comprises the plurality of precursor ions and the iterative exclusion list comprises a precursor ion that has been previously fragmented from the plurality of precursor ions, and
wherein the sample comprises a cell culture medium from a mammalian cell-based antibody production system.