Patent ID: 11882808
Assignee: PAIRWISE PLANTS SERVICES, INC.
Field: Biotechnology (Chemistry)
Classification: CPC A  C | IPC A  C

Claim 1:
2. A soybean plant cell comprising at least one non-natural mutation in each of an endogenous STAYGREEN1 (SGR1) gene and an endogenous STAYGREEN2 (SGR2) gene, wherein the at least one non-natural mutation is introduced into the untranslated region or the promoter region of the endogenous SGR gene and the endogenous SGR2 gene using an editing system that comprises a nucleic acid binding domain that binds to a target site in the endogenous SGR1 gene and/or the endogenous SGR2 gene, and the at least one non-natural mutation in the SGR1 gene results in the nucleic acid sequence of any one of SEQ ID NOs:116, 119, 121, 124, 128, 129, 132, 135, 138, 139, 141, 142 or 144 and the at least one non-natural mutation in the SGR2 gene results in the nucleic acid sequence of any one of SEQ ID NOs:115, 117, 118, 120, 122, 125, 126, 127, 130, 131, 134, 136, 137, 140, or 143; and wherein a plant regenerated from the soybean cell and comprising the at least one non-natural mutation in the endogenous SGR1 gene and in the endogenous SGR2 gene exhibits an increase in resistance to soybean rust, delayed senescence and/or chlorophyll retention as compared to a control soybean plant not comprising the at least one non-natural mutation in the endogenous SGR1 gene and in the endogenous SGR2 gene.