Patent ID: 11913944
Assignee: PROMEGA CORPORATION
Field: Measurement (Instruments)
Classification: CPC G | IPC G

Claim 8:
9. A method of capturing a cellular target protein comprising:
contacting a cell containing the target protein with a PRG/HA probe of claim 1, wherein the bioactive agent is capable of binding the target protein;
allowing the PRG/HA probe to enter the cell;
allowing the bioactive agent to bind the target protein;
irradiating the cell with a wavelength of UV light that converts the PRG into a reactive form of the PRG, wherein the reactive form of the PRG forms a covalent bond with the target protein;
lysing the cell to form a lysate;
contacting the lysate with a solid-surface-immobilized modified dehalogenase enzyme that forms a covalent bond with a haloalkane substrate upon association therewith;
allowing the modified dehalogenase enzyme to covalently bind the haloalkane of the PRG/HA probe; and
removing the solid surface from the lysate and/or washing non-immobilized components of the lysate from the solid surface.