Patent ID: 11926864
Assignee: ROCHE SEQUENCING SOLUTIONS, INC.
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 0:
1. A method for barcoding, comprising:
(a) providing a population of fixed cells or cell organelles in a first reaction volume;
(b) hybridizing oligonucleotide probes to target molecules that are in or on the fixe cells or cell organelles in the first reaction volume;
(c) splitting the population of fixed cells or cell organelles into a plurality of second reactions volumes, wherein at least some of the second reaction volumes receive a single fixed cell or cell organelle from the population of fixed cells or cell organelles; and
(d) adding cell-specific nucleic acid barcodes onto: (i) the oligonucleotide probes, (ii) ligation products comprising the oligonucleotide probes, or (iii) complements of the oligonucleotide probes or ligation products, in the plurality of second reaction volumes.