Patent ID: 11905534
Assignee: SHANGHAI QIZHOU ZIYUE BIOTECHNOLOGY CO., LTD
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 3:
4. The method of claim 2, wherein the L-glutamate dehydrogenase mutant has a concentration of 0.05-3 U/m;
and/or, the inorganic amino donor has a concentration of 100-2000 mM;
and/or, the 2-oxo-4-(hydroxymethylphosphinyl) butyrate has a concentration of 100-600 mM;
and/or, the mass ratio of the reduced coenzyme NADPH to the 2-oxo-4-(hydroxymethylphosphinyl) butyrate is 1:100-1:20000;
and/or, the inorganic amino donor is one or more of ammonia, ammonium sulfate, ammonium chloride, diammonium hydrogen phosphate, ammonium acetate, ammonium formate and ammonium bicarbonate;
and/or, the reaction solvent is water;
and/or, the amination reaction has a reaction system with a pH of 7-9;
and/or, the amination reaction has a reaction system with a temperature of 20-50° C.