Patent ID: 11911433
Assignee: JIANGSU DEHE BIOTECHNOLOGY CO., LTD.
Field: Pharmaceuticals (Chemistry)
Classification: CPC A | IPC A

Claim 0:
1. A preparation method of non-ester tea polyphenols rich in epigallocatechin (EGC), characterized by comprising the steps of:
(1) pretreatment of tea leaves: using green tea leaves as raw materials, crushing and wall-breaking the tea leaves to obtain tea particles;
(2) endogenous enzymes enzymolysis: adding the pre-treated tea particles into a dynamic extraction tank, adding deionized water, and adding a food-grade acid to adjust the pH to 2.0-6.5; and under stirring conditions, performing enzymolysis on the ester tea polyphenols in the pre-treated tea particles by the hydrolytic enzymes of the tea itself;
(3) leaching: leaching the pre-treated tea particles after polyphenols with deionized water twice, filtering after each leaching, and combining the two filtrates;
(4) filtration: cooling the above-mentioned filtrate, filtering again, and collecting the filtrate;
(5) concentration: concentrating the filtrate in step (4);
(6) mobile extraction-water washing: extracting the concentrated solution in step (5) with 1-2 times volume of ethyl acetate, collecting an ethyl acetate phase, and concentrating the ethyl acetate phase to a solid content of 2-10%; then performing reverse phase extraction with 0.2-1 times volume of deionized water on the concentrated ethyl acetate phase, collecting a water-washed phase, and performing reverse phase extraction with the same method for 1-5 times, and combining the water-washed phases; and then performing extraction with 1-2 times volume of ethyl acetate for the water-washed phase, and collecting the ethyl acetate phase;
(7) conversion and dissolving: concentrating the ethyl acetate phase finally collected in step (6) to an extractum, and then adding deionized water for dissolution to obtain a tea polyphenol aqueous solution; and
(8) concentration: concentrating the aqueous tea polyphenol solution in step (7) under vacuum;
(9) drying: drying the concentrated solution in step (8) by spray drying to obtain a non-ester tea polyphenol product rich in EGC.