Patent ID: 11965192
Assignee: CODEXIS, INC.
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 5:
6. A method for producing phenyl acetate mono-protected or di-protected insulin, comprising: i) providing an engineered penicillin G acylase having a polypeptide sequence that is at least 95% identical to SEQ ID NO: 1220, wherein said penicillin G acylase comprises a leucine residue at position X2 as compared to the sequence of SEQ ID NO: 1220 and insulin; and ii) exposing said engineered penicillin G acylase to said insulin, under conditions such that said engineered penicillin G acylase adds up to two tri-phenyl acetate protecting groups selected from the group consisting of the A1, B1 and B29 tri-phenyl acetate protecting groups to said insulin thereby producing phenyl acetate mono-protected or di-protected insulin.