Patent ID: 11904051
Assignee: UNIVERSITäT FREIBURG
Field: Pharmaceuticals (Chemistry)
Classification: CPC A | IPC A

Claim 9:
10. A method of preparing a mechanoresponsive vesicle comprising the steps of
providing 1,3-diheptadecanam idopropan-2-yl(2-(trimethylammonio)ethyl) phosphate dissolved in an organic solvent;
removing said organic solvent under an inert atmosphere thereby obtaining a lipid sheet;
solving said lipid sheet in a first aqueous buffer solution having a physiological pH;
applying at least one freezing step and one thawing step;
extrusion of said suspension, thereby obtaining an extrudate; and
dialyzing the extrudate in a second buffer solution having a physiological pH to remove small molecular weight components.