Patent ID: 11927591
Assignee: GEORGETOWN UNIVERSITY
Field: Measurement (Instruments)
Classification: CPC G  C | IPC C  G

Claim 0:
1. A method for identifying a neopeptide in or on a population of cells that is a marker of abnormal cells, the method comprising
a) sequencing long-read messenger RNA (mRNA) isolated from a first subset of the population cells,
b) identifying splice variants generated from the sequenced long-read mRNA,
c) determining in a second subset of the population of cells presence of neopeptides that are translated from the identified splice variants and determining the presence of the neopeptide in a population of normal cells, wherein a single identified splice variant translates to a single neopeptide,
wherein, the presence of the neopeptide in the second subset of the population cells and absence of the neopeptide in the normal cells is indicative that the neopeptide is a marker for abnormal cells;
wherein the neopeptide comprises a novel splice variant; and
wherein the population of cells is a population of cancer cells, and the population of normal cells is a population of non-cancer cells of the same tissue origin as the population of cells.