Patent ID: 11959094
Assignee: SALK INSTITUTE FOR BIOLOGICAL STUDIES
Field: Pharmaceuticals (Chemistry)
Classification: CPC C  A | IPC A  C

Claim 0:
1. Recombinant vectors configured to integrate an exogenous DNA sequence into a target nucleic acid sequence in a genome of a cell, wherein the vectors collectively encode:
a targeting construct comprising the exogenous DNA sequence and a target sequence entirely in reverse orientation with respect to the exogenous DNA sequence;
a complementary strand oligonucleotide homologous to the target sequence;
a nuclease capable of producing double stranded DNA blunt end cuts;
wherein the exogenous DNA sequence comprises at least one nucleotide difference compared to the genome, both the target sequence and the target nucleic acid sequence in the genome are recognized and cut by the nuclease, and the recombinant vectors are configured such that that the target nucleic acid sequence in the genome is no longer present once the exogenous DNA sequence has been integrated into the genome in the correct orientation.