Patent ID: 11905316
Assignee: CHINA AGRICULTURAL UNIVERSITY
Field: Biotechnology (Chemistry)
Classification: CPC C  A  Y | IPC C

Claim 0:
1. A method of increasing maize density tolerance, comprising a step of increasing an abundance of a ZmROA1 protein in a maize plant by overexpression of a gene encoding the ZmROA1 protein, wherein a ZmROA1 protein overexpressed has the amino acid sequence shown in SEQ ID NO: 3, and the ZmROA1protein overexpression comprises inserting a gene encoding the ZmROA1 protein comprising the nucleotide sequence shown in SEQ ID NO: 1 between two XcmI restriction sites of a pBCXUN expression vector comprising a promoter of the Ubiquitin1 gene; transforming an immature maize plant embryo; expressing the recombinant ZmROA1 gene; producing overexpression maize lines and selecting a transformed maize plant from the overexpression maize lines that exhibits a reduced root angle, improved density tolerance, reduced plant height, and reduced ear height as compared to control maize plant not comprising said overexpression of the recombinant ZmROA1 gene.