Patent ID: 11913046
Assignee: INBIOSE N.V.
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 0:
1. A genetically engineered yeast cell, wherein:
the cell comprises:
a) at least one heterologous nucleic acid molecule encoding a transporter protein that facilitates export of 2′ fucosyllactose from the yeast cell, wherein the transporter protein is selected from the group consisting of
a Sugars Will Eventually be Exported Transporter (SWEET) family transporter having at least 90% identity to a peptide selected from the group consisting of SEQ ID NO: 93, 94, 95, and 96,
a Sugar Efflux Transporter A (SetA) family transporter having at least 90% identity to a peptide selected from the group consisting of SEQ ID NO: 88, 105, 106, 107, and 108, and
a sugar porter family transporter having at least 90% identity to a peptide selected from the group consisting of SEQ ID NO: 65 and SEQ ID NO: 66;
b) at least one heterologous nucleic acid molecule encoding a guanosine diphosphate (GDP)-mannose-4,6-dehydratase (EC 4.2.1.47);
c) at least one heterologous nucleic acid molecule encoding a GDP-4-keto-6-D-deoxymannose epimerase-reductase (EC 1.1.1.271); and
d) at least one heterologous nucleic acid molecule encoding a 2-α-L-fucosyltransferase (EC 2.4.1.69); and

said yeast cell produces 2′ fucosyllactose.