Patent ID: 11885811
Assignee: REGENERON PHARMACEUTICALS, INC.
Field: Measurement (Instruments)
Classification: CPC G  B  H | IPC G  H

Claim 5:
6. A method for determining intact masses of at least on peptide or protein in a sample, comprising:
contacting the sample to a solid surface, wherein the solid surface comprises a hydrophobic group and the solid surface is included in a chromatography column;
washing the solid surface using a mobile phase to produce at least one eluent, wherein the eluent comprises the at least one peptide or protein;
determining the intact masses of the at least one peptide or protein in the at least one eluent using a mass spectrometer under native conditions; and
identifying or quantifying a drug location, a positional isomer or a degraded payload of the antibody-drug conjugate based on the at least one separation profile or a comparison with another separation profile;
wherein the mass spectrometer is coupled online to the chromatography column.