Patent ID: 11970693
Assignee: NAUTILUS SUBSIDIARY, INC.
Field: Measurement (Instruments)
Classification: CPC C  G | IPC C  G

Claim 0:
1. A method for selecting an affinity reagent which binds a desired peptide epitope in a plurality of sequence contexts, the method comprising:
obtaining a peptide library comprising a plurality of at least 400 sequence contexts, each peptide of the peptide library comprising the sequence αXβ, wherein X is the desired peptide epitope, wherein X is a sequence of 2, 3 or 4 amino acids, wherein each of a and comprises at least one amino acid, and wherein each of α and β varies amongst the plurality of sequence contexts within the peptide library; and
screening one or more affinity reagents against the peptide library to select an affinity reagent from among the one or more affinity reagents which binds the desired peptide epitope X in at least 10% of the plurality of sequence contexts, wherein the selected affinity reagent is selected from the group consisting of an antibody, an aptamer, a peptamer, and an antibody fragment.