Patent ID: 11939630
Assignee: SHAANXI LIFEGEN CO., LTD.
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 6:
7. A real-time fluorescent PCR method for detecting HLA-B*15:02 allele for non-disease diagnostic purposes, comprising the following steps:
1) Acquiring the genomic DNA of a sample to be tested;
2) Adding the genomic DNA of the sample to be tested, a specific primer probe combination according to claim 2 and PCR reagents in a HLA-B*15:02 alleles positive reaction system and a HLA-B*15:02 alleles negative reaction system respectively;
3) Detecting the reaction system by fluorescence PCR, collecting fluorescence signal of the 6-carboxy-fluorescein fluorescence channel; and
4) judging the conditions of the HLA-B*15:02 allele carried by the sample to be tested by the fluorescence signal results.