Patent ID: 11912999
Assignee: UNIVERSITY OF FLORIDA RESEARCH FOUNDATION, INC.
Field: Pharmaceuticals (Chemistry)
Classification: CPC C  A | IPC A  C

Claim 2:
3. The aptamer-NHCM conjugate of claim 1, wherein the modified carbene is independently selected from the group consisting of:, each R group is independently H, C1-C18 alkyl, C2-C18 alkenyl, C2-C18 alkynyl, C6-C14 aryl, C7-C18 arylalkyl, C8-C18 arylalkenyl, C8-C18 arylalkynyl, C1-C18 alkoxy, C6-C14 aryloxy, C7-C18 arylalkyloxy, C2-C18 alkenyloxy, C2-C18 alkynyloxy, C8-C18 arylalkenyloxy, C8-C30 arylalkynyloxy, C2-C30 alkylester, C7-C15 arylester, C8-C30 alkylarylester, C3-C18 alkenylester, C3-C18 alkynylester, C3-C18 di- or poly-ether, C3-C18 di- or poly-etherester, C3-C18di- or poly-ester, C3-C18 di- or poly-amine, or C4-C18di- or poly-ene, and is optionally substituted or multiply substituted with any of Cl, Br, I, F, OH, R′2N, R′SO2, R′SO, R′S, R′3Si, R′O, NH2, C(O)OH, N3, C═CH, vicinal disubstituted with C(O)OC(O), a cyclic conjugated diene, any salts derived therefrom or any condensation or addition derivative substituent therefrom;
each R′ is independently C1-C18 alkyl, C2-C18 alkenyl, C2-C18 alkynyl, C6-C14 aryl, C7-C18 arylalkyl, C8-C18 arylalkenyl, C8-C18 arylalkynyl, C1-C18 alkoxy, C6-C14 aryloxy, C7-C18 arylalkyloxy, C2-C18 alkenyloxy, C2-C8 alkynyloxy, C8-C18 arylalkenyloxy, C8-C30 arylalkynyloxy, C2-C30 alkylester, C7-C15 arylester, C8-C30 alkylarylester, C3C18 alkenylester, C3-C18 alkynylester, C3-C18 di- or poly-ether, C3-C18 di- or poly-etherester, C3-C18 di- or poly-ester, C3-C18 di- or poly-amine, or C4-C18 di- or poly-ene, and each R and R′ group is linear, branched, cyclic, polycyclic or any combination thereof;
at least one of the R groups comprises the condensation or addition derivative substituent comprising the coupling unit forming the hydrolytically stable bond between the modified carbene and the aptamer;
the condensation or addition derivative substituent is derived from condensation or addition of a —NH2 and —C(O)OH, —N3 and —C═CH; —NH2 and vicinal disubstituted —C(O)CO(O)—, or homocyclic or heterocyclic conjugated diene and —C═CH or —HC═CH2; and
the modified carbene is achiral, a racemic mixture, partially resolved enantiomer, resolved enantiomer, resolved diastereomers, or a mixture of diastereomers.