Patent ID: 11965191
Assignee: CALIFORNIA INSTITUTE OF TECHNOLOGY
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 5:
6. The synthetic protein circuit of claim 1, wherein
the third protease comprises a third protease first cut site specific for the first protease, and configured to be deactivated by cleavage of the third protease first cut site; and
the fourth protease comprises a fourth protease first cut site specific for the second protease, and configured to be deactivated by cleavage of the fourth protease first cut site;
wherein the first target protein comprises a first cut site specific for the third protease and a second cut site specific for the fourth protease between the first degron of the first target protein and a distal part of the first target protein from the first degron, and wherein the first target protein is stabilized by cleavage of the first cut site specific for the third protease and the second cut site specific for the fourth protease.