Patent ID: 11866724
Assignee: GENE BRIDGES GMBH
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 3:
4. The method of claim 1, further comprising the generation of an adenoviral vector from the circular medium-copy plasmid comprising the full length adenoviral genome sequence, comprising:
a) providing a second linear nucleic acid molecule which shares at least two regions of sequence homology with the circular medium-copy plasmid comprising the full length adenoviral genome sequence, wherein the second linear nucleic acid molecule comprises one or more transgenes of interest situated between two regions of sequence homology; and
b) bringing the circular medium-copy plasmid comprising the full length adenoviral genome sequence and the second linear nucleic acid molecule into contact in the presence of a 5′ to 3′ exonuclease and an annealing protein such that sequences between the regions of homology in the second linear nucleic acid molecule are introduced into the circular medium-copy plasmid;
wherein:
i) the 5′ to 3′ exonuclease is RecE and the annealing protein is RecT; or
ii) the 5′ to 3′ exonuclease is Red alpha and the annealing protein is Red beta.