Patent ID: 11925166
Assignee: MARS, INCORPORATED
Field: Pharmaceuticals (Chemistry)
Classification: CPC A | IPC A

Claim 4:
5. A method for producing a Theobroma secondary metabolite, comprising:
obtaining an explant from a young Theobroma leaf, wherein the Theobroma is Theobroma cacao; 
sterilizing the explant;
inducing friable callus from the explant on a callus induction medium, wherein the callus induction medium comprises:
a base plant medium;
1-naphthalene acetic acid (NAA) at a concentration of 1 mg/L; and
6-benzylaminopurine (BAP) at a concentration from 0.5 mg/L to 1 mg/L;

proliferating the transformed friable callus on a callus proliferation medium,
wherein the callus proliferation medium comprises: the base plant medium and tidiazuron, and
wherein the base plant medium comprises:
woody plant medium salts;
vitamins;
L-glutamine;
myo-inositol; and
sucrose;

suspending the proliferated callus in a suspension medium, wherein the suspension medium comprises:
salts;
vitamins;
L-glycine;
sucrose;
penicillin;
2,4-dichlorophenoxyacetic acid (2,4-D); and
kinetin (KIN); and
recovering a secondary metabolite from the suspension medium.