Patent ID: 11926845
Assignee: HITACHI, LTD.
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 0:
1. A cell culture method for inducing differentiation of a pluripotent stem cell into a trophoblast, the cell culture method comprising:
a first step of seeding the pluripotent stem cell on a cell culture substrate including a planar mesh made of a first metal and coated with at least one of Au or parylene, and culturing the pluripotent stem cell adhered to the cell culture substrate in a presence of a ROCK inhibitor at a concentration of 1 μM to 100 μM during a first time period from a start of culturing to Day 1 after the start of culturing;
a second step of culturing the cell adhered to the cell culture substrate, which has been subjected to the first step, without the ROCK inhibitor during a second time period extending from an end of the first time period to a time that is from 9 to 13 days after the start of the culturing; and
a third step of culturing the cell adhered to the cell culture substrate, which has been subjected to the second step, in the presence of the ROCK inhibitor at a concentration of 1 μM to 200 μM during a third time period extending from an end of the second time period to a time that is from 4 to 12 days after the end of the second time period, wherein
the pluripotent stem cell has differentiated into the trophoblast during at least one of the second step or the third step.