Patent ID: 11920180
Assignee: TOHOKU UNIVERSITY
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 0:
1. A method for inducing differentiation of Multilineage-differentiating Stress Enduring (MUSE) cells as pluripotent stern cells in vitro into cells having the same phenotype and function as Information Presenting Cells for Differentiation (IPCD), the method comprising co-culturing the pluripotent stem cells or cell population with high content of the pluripotent stem cells either with damaged and/or dead cells as IPCD, and/or fragments of said damaged and/or dead cells,
wherein the pluripotent stem cells phagocytize the damaged and/or dead cells as IPCD, and/or fragments of said damaged and/or dead cells,
wherein the resulting differentiated cells exhibit characteristics of the IPCD,
the pluripotent stern cells are SSEA-3 positive and CD105-positive,
the pluripotent stem cells have all of the following properties:
(i) telomerase activity at low or under detection level;
(ii) having the ability to differentiate into cells of three germ layers;
(iii) exhibiting no tumorigenic proliferation; and
(iv) having self-renewal ability, and

the pluripotent stem cells are:
(v) CD117-negative and CD146-negative,
(vi) CD117-negative, CD146-negative, NG2-negative, CD34-negative, vWF-negative, and CD271-negative, or
(vii) CD34-negative, CD117-negative, CD146-negative, CD271-negative, NG2-negative, vWF-negative, Sox10-negative, Snail-negative, Slug-negative, Tyrp1-negative, and Dct-negative.