Patent ID: 11926857
Assignee: CODEXIS, INC.
Field: Biotechnology (Chemistry)
Classification: CPC C  Y | IPC C

Claim 3:
4. The engineered variant LovD polypeptide of claim 1, which further comprises:
(i) at least one substitution selected from the group consisting of M157V, S164G, S172N, L174F, A178L, N191G, L1921, A247S, R250K, S256T, A261H, Q297G, L361M, V3701 and N391S;
(ii) at least one substitution selected from the group consisting of Q124M, A261H, Q295R AND Q412R;
(iii) at least one substitution selected from the group consisting of N43R, D96R and H404K;
(iv) at least one substitution selected from the group consisting of C40R, C60R and D245E; and
(v) at least one substitution selected from the group consisting of 14N, A9V, K26E, R28K, R28S, 135L, C40A, C40V, C40F, S41R, N43Y, C60F, C60Y, C60N, C60H, S109C, S142N, A184T, A184V, N191S, A261T, A261E, A261V, L292R, Q297E, L335M, A377V, A383V, N391D and H404R.