Patent ID: 11927594
Assignee: RENSSELAER CENTER FOR TRANSLATIONAL RESEARCH, INC.
Field: Measurement (Instruments)
Classification: CPC G | IPC G

Claim 0:
1. A method comprising:
a) obtaining a test sample of tissue from a subject comprising
i) obtaining a tissue sample from a human subject, wherein the tissue sample contains a buffy coat; and
ii) isolating a protein from the tissue sample to provide a sample of the protein;

b) assaying the test sample to quantify in the test sample a phosphorylation level of a peptide comprising
i) adding to the sample of the protein extracted from the buffy coat an amount of a quantification standard to provide the test sample; and
ii) assaying the test sample to quantify in the test sample a wild type phosphopeptide of the protein extracted from the buffy coat, wherein the quantifying is based on the amount of the quantification standard, and wherein the quantification standard is a form of the phosphopeptide that is isotopically enriched to a non-natural abundance of an isotope;

c) comparing the phosphorylation level of the peptide to a phosphorylation level of the corresponding peptide in the test sample to a control sample;
d) based on the comparing the phosphorylation level of the peptide to the phosphorylation level of the corresponding peptide in the test sample to the control sample, determining whether the subject suffers from a pulmonary condition; and
e) based on the determining whether the subject suffers from the pulmonary condition, administering to the subject a pharmaceutical compound for treatment of the pulmonary condition;
wherein the pulmonary condition is pulmonary arterial hypertension;
and wherein the quantification standard is an isotopically-enriched peptide selected from the following:, SEQ

ID

No.
Protein
Sequence

 1
IKZF3
GLSPNNSGHDSTDTDSNHEER

 

 2
HMHA1
HASAAGFPLSGAASWTLGR

 

 3
BCAS3
HGSYDSLASDHSGQEDEEWLSQVEIVTHTGPHR

 

 4
RHG25
RTQTLPNRK

 

 5
NUMA1
RQSMAFSILNTPK

 

 6
LEUK
RPTLTTFFGR

 

 8
NU214
TPSIQPSLLPHAAPFAK

 

 9
WDR24
IIYCSPGLVPTANLNHSVGK

 

10
NUMA1
RASMQPIQIAEGTGITTR

 

14
MK14
HTDDEMTGYVATR

 

15
ZN404
HSHLTEHQK.