Patent ID: 11953436
Assignee: PROTEINSIMPLE
Field: Measurement (Instruments)
Classification: CPC G | IPC A  B  C  G

Claim 10:
11. A system, comprising:
a memory; and
a processor operatively coupled to the memory, the processor configured to:
receive a fluorescence signal associated with a first set of separated protein isoforms, the first set of separated protein isoforms being obtained from a first portion of a sample containing a plurality of protein isoforms, at least some protein isoforms from the plurality of protein isoforms having one or more free thiol groups, the fluorescence signal being associated with a fluorescent detection binder applied to the first sample portion that is configured to bind to free thiol groups,
receive a first absorbance signal associated with the first set of separated protein isoforms,
receive a second absorbance signal associated with a second portion of the sample,
identify, based on the first absorbance signal and the second absorbance signal, at least a subset of the protein isoforms from the sample,
calculate, based on the fluorescence signal, a quantity of free thiol groups associated with each protein isoform from the subset of the protein isoforms.