Patent ID: 11917960
Assignee: COMMONWEALTH SCIENTIFIC AND INDUSTRIAL RESEARCH ORGANISATION
Field: Biotechnology (Chemistry)
Classification: CPC A  C | IPC A  C

Claim 0:
1. A method for producing genetically modified differentiating embryogenic callus (DEC) tissue of sorghum, the method comprising: (1) preparing differentiating embryogenic callus (DEC) tissue of sorghum, comprising culturing isolated immature embryos (IEs) from sorghum in callus inducing medium (CIM) for a time and under conditions sufficient to produce DEC tissue from the IEs, wherein the CIM comprises a basal medium suitable for culturing plant cells supplemented with one or more auxins, one or more cytokinins and one or more agents which reduces oxidative browning, wherein the one or more agents which reduce oxidative browning comprise lipoic acid at a concentration of 0.5 mg/L to 2.0 mg/L, and (2) introducing one or more nucleic acids into the DEC tissue of sorghum to produce a genetically modified sorghum DEC tissue.