Patent ID: 11901042
Assignee: nan
Field: Computer technology (Electrical engineering)
Classification: CPC G  C | IPC C  G

Claim 0:
1. A method of comparing detection indices of two or more tests performed separately, each test comprises;
(b) using a clinical specimen carrying cancer cells with heterozygous alleles for a somatic mutation, and
(c) viewing in a microscopic field counting total number of cells and the total number of cancer cells, and
dividing the total number of cancer cells by the total number of cells, generating the tumor content, and
multiplying the tumor content by 100 to determine the percentage tumor content, and

(d) extracting the total DNA from the clinical sample, and
using spectrophotometer to determine the DNA concentration (ng/ul) of the DNA extract, and
multiplying volume (ul) of DNA extract added to the reaction by the DNA concentration to determine the amount of DNA, and

(e) adding up the volumes of reagents added to the reaction to determine the reaction volume (ul), and
(e) calculating the number of copies of the mutant allele (MC) per milliliter by, Amount
   ⁢
      
   of
   ⁢
      
   DNA
   ×
   290
   ×
   %
   ⁢
      
   tumor
   ⁢
      
   content
  
  
   Reaction
   ⁢
      
   volume
   ⁢
      
   
    (
    
     μ
     ⁢
     l
    
    )
   
   ×
   100
  
 

(f) calculating number of copies of wild type allele (WC) per milliliter by, (
       
        Amount
        ⁢
           
        of
        ⁢
           
        DNA
        ×
        290
        ×
        %
        ⁢
           
        tumor
        ⁢
           
        content
       
       )
      
      +
     
    
   
   
    
     
      [
      
       
        (
        
         
          Amount
          ⁢
             
          of
          ⁢
             
          DNA
          ×
          290
          ×
          100
         
         -
         
          %
          ⁢
             
          Tumor
          ⁢
             
          content
         
        
        )
       
       ×
       2
      
      ]
     
    
   
  
  
   Reaction
   ⁢
      
   
    volume
    (
    
     μ
     ⁢
     l
    
    )
   
   ×
   100
  
 

and
(g) determining the detection index by multiplying the inverse of (MC) and inverse of the ratio of (MC:WC), and
the test with higher detection index is more sensitive in detecting the somatic mutation.