Patent ID: 11885777
Assignee: ANHUI AGRICULTURAL UNIVERSITY
Field: Measurement (Instruments)
Classification: CPC G | IPC G

Claim 3:
4. The method for purifying and detecting 6-hydroxynobilonine in fresh stems of Dendrobium huoshanense according to claim 1, wherein in the step S4, the specific operation of the MCX extraction column is as follows:
the filtrate, after concentration, is dissolved with an aqueous solution comprising 2-5% H3PO4 by mass and then passed through a 0.22 μm water filter membrane to obtain an injection solution; activating the MCX extraction column with 5-8 ml of methanol and 5-8 ml of water in sequence; injecting the injection solution at a flow rate of 0.5-1.0 ml/min; leaching the column with 3-5 mL of an aqueous solution comprising 2-5% formic acid by mass; and after leaching, the column is eluted with a methanol/acetonitrile solution containing 3-5% of ammonium hydroxide by volume to obtain eluent.