Patent ID: 11959134
Assignee: BIONANO GENOMICS, INC.
Field: Computer technology (Electrical engineering)
Classification: CPC C  G | IPC C  G

Claim 0:
1. A method of analyzing nucleic acid molecules without compromising strand phosphodiester bond integrity, comprising:
providing a plurality of nucleic acid molecules comprising a plurality of occurrences of a DNA repeated motif, wherein an input coverage is 50-fold or less, wherein the DNA repeated motif comprises a methyltransferase recognition sequence and the DNA repeated motif is selected to have an average repeat frequency of about 5 sites to about 35 sites per 100 Kb in the plurality of nucleic acid molecules;
covalently labeling the plurality of nucleic acid molecules in a sequence-specific fashion at the DNA repeated motif with a label, wherein the covalent labeling is effected with a methyltransferase in the presence of a modified cofactor, and wherein the modified cofactor comprises modified S-adenosyl methionine (SAM), and wherein the modified cofactor comprises a tag that is detectable, transferable, or both; and
detecting the label to identify patterns of the DNA repeated motif of the plurality of nucleic acid molecules,
wherein the covalently labeled nucleic acid molecules have a length of at least 150 kb and wherein the strand phosphodiester bonds of the plurality of nucleic acid molecules are intact.