Patent ID: 11965191
Assignee: CALIFORNIA INSTITUTE OF TECHNOLOGY
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 10:
11. The synthetic protein circuit of claim 1
wherein the third protease comprises a third protease first cut site specific for the first protease, and configured to be deactivated by cleavage of the third protease first cut site;
wherein the first target protein further comprises a first cut site specific for the third protease between the first degron and a distal part of the first target protein from the first degron of the first target protein, and a second cut site specific for the second protease connected to a second degron of the first target protein and an optional association domain of the first target protein, and wherein the first target protein is stabilized by cleavage of both of the first cut site specific for the third protease and the second cut site specific for the second protease.