Patent ID: 11969497
Assignee: EXSYMOL
Field: Pharmaceuticals (Chemistry)
Classification: CPC A | IPC A

Claim 0:
1. A process for producing an extract of seeds of the genus Entada enriched in entadamide A, comprising:
(i) activating endogenous enzyme(s) by dispersion of the seeds under mechanical stirring in a seed/water weight ratio of about 0.02 to about 2;
(ii) enzymatically hydrolyzing the dispersion obtained in step (i) by heat treatment at a temperature between about 25 and about 100° C. and for a time period of between about 2 minutes and about 12 hours, at a pH of between about 4 and about 8;
(iii) inhibiting the enzymatic activity in the dispersion obtained in step (ii), the inhibition of said enzymatic activity comprising:
a) adding an agent irreversibly inhibiting the activity of the endogenous enzymes in a volume ratio of inhibiting agent/water of between about 0.1 and about 10;
b) heating the reaction medium obtained in step (iii/a) at a temperature of between about 25 and about 75° C. and for a time period of between about 2 minutes and about 12 hours;
c) removing the inhibiting agent under reduced pressure to obtain an aqueous filtrate enriched in entadamide A and containing a native non-hydrolyzed phaseoloidin;
(iv) adding a cosmetically acceptable solvent to the aqueous filtrate obtained in step (iii/c), then adjusting the pH between about 3 and about 7.