Patent ID: 11945840
Assignee: OXFORD NANOPORE TECHNOLOGIES PLC
Field: Measurement (Instruments)
Classification: CPC C  B  G | IPC A  B  C

Claim 9:
10. An isolated pore complex according to claim 1, wherein the CsgG pore comprises at least one monomer comprising one or more modification corresponding to the following modifications in SEQ ID NO:3:
(i) a modification at one or more positions Y51, N55 and F56;
(ii) at least one substitution selected from R97W or R97Y and R93W or R93Y;
(iii) deletion of V105, A106 and 1107 of SEQ ID NO:3;
(iv) deletion of one or more of positions R192, F193, 1194, D105, Y196, Q197, R198, L199 and E201 of SEQ ID NO:3;
(v) at least one substitution selected from K94N/Q/R/F/Y/W/L/S, D43S, E44S, F48S/N/Q/Y/W/I/V/H/R/K, Q87N/R/K, N91K/R, R97F/Y/W/V/I/K/S/Q/H, E101I/L/A/H, N102K/Q/L/I/V/S/H, R110F/G/N, Q114R/K, R142Q/S, T150Y/A/V/L/S/Q/N;
(vi) a modification at one or more of positions 141, R93, A98, Q100, G103, T104, A106, 1107, N108, L113, S115, T117, Y130, K135, E170, S208, D233, D238, E244, Q42, E44, L90, N91, N195, A99, E101 and Q114;
(vii) at least one substitution selected from Y51A/I/V/S/T, N55A/I/V/S/T and F56/A/I/V/S/T/Q;
(viii) the substitution R97W;
(ix) deletion of F193, 1194, D195, Y196, Q197, R198 and L199 or deletion of D195, Y196, Q197, R198 and L199;
(x) deletion of V105, A106 and 1107;
(xi) a substitution selected from K94Q and K94N;
(xii) at least one substitution selected from Q42K or Q42R; E44N or E44Q; L90R or L90K; N91R or N91K; I95R or I95K; A99R or A99K; E101H, E101K, E101N, E101Q or E101T; and/or Q114K;
(xiii) the substitution N55V; and/or
(xiv) R or K at a position corresponding to R192.