Patent ID: 11912823
Assignee: GENENTECH, INC.
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 0:
1. A method of producing a polypeptide in a cell culture, comprising the step of culturing a cell that produces the polypeptide in a cell culture medium under conditions suitable for production of the polypeptide, wherein the cell culture medium comprises a poloxamer prepared by a method comprising:
(a) heating a solid poloxamer to form a liquid poloxamer, wherein the solid poloxamer is heated to:
(1) between 157° C. and 185° C. for at least 1 minute,
(2) between 134° C. and 157° C. for at least 1 minute,
(3) between 120° C. and 134° C. for at least 62 minutes,
(4) between 100° C. and 120° C. for at least 98 minutes,
(5) between 80° C. and 100° C. for at least 122 minutes, or
(6) between 60° C. and 80° C. for at least 143 minutes; and

(b) cooling the liquid poloxamer to a temperature below 50° C. to form a solid heat-treated poloxamer, wherein the cooling is not conducted in a prilling or milling device, wherein the poloxamer comprises a copolymer of ethylene oxide and propylene oxide; and, wherein the poloxamer comprises a copolymer having a formula of HO(C2H4O)n(C3H6O)m(C2H4O)nH with n having a value of 80, with m having a value of 27, and the poloxamer has an average molecular weight of from 7680 to 9510 g/mol.