Patent ID: 11952617
Assignee: KASA BIO, L.L.C.
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 24:
25. The method of claim 24, wherein the RNA is miRNA, wherein reverse transcription of the miRNA comprises (a) bringing a sample comprising the miRNA into contact with a single-stranded RT primer and optionally a blocker oligonucleotide under conditions suitable for the RT primer to hybridize to the target miRNA,
wherein the RT primer comprises in the 5′ to 3′ direction, (i) a tag collectively comprising a universal reverse primer sequence and a binding site for the blocker oligonucleotide, and (ii) a sequence complementary to the 3′-end of the target miRNA; and (b) performing reverse transcription to obtain cDNA.