Patent ID: 11970699
Assignee: COUNCIL OF SCIENTIFIC & INDUSTRIAL RESEARCH
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 0:
1. A ribonucleoprotein complex for gene editing comprising:
(a) an engineered FnCas9 protein effector selected from the group consisting of SEQ ID NO:16 and SEQ ID NO:31; and
(b) a crispr RNA (crRNA) having from 20 to 24 nucleotides, wherein the crRNA binds to a nucleotide sequence located upstream and adjacent to a proto-spacer adjacent motif (PAM) sequence selected from the group consisting of NGG, NGA, GGA, and GGG; and
(c) a trans-activating crispr RNA (tracrRNA) having SEQ ID NO:305.