Patent ID: 11946937
Assignee: MAYO FOUNDATION FOR MEDICAL EDUCATION AND RESEARCH
Field: Measurement (Instruments)
Classification: CPC G  A | IPC G

Claim 12:
13. A method of monitoring a treatment of a disorder in a patient, wherein said disorder is associated with increased apoptosis inhibitor of macrophage (AIM) polypeptide levels, said method comprising:
providing an initial sample comprising immunoglobulins from the patient, wherein said initial sample is obtained from the patient prior to the treatment; providing one or more secondary samples comprising immunoglobulins, wherein said one or more secondary samples are obtained from the patient during the treatment, after the treatment, or both;
immunopurifying IgM immunoglobulins from the sample, wherein the immunopurifying step comprises an elution under conditions suitable to release AIM polypeptides bound to the IgM immunoglobulins into the immunopurified sample;
subjecting the immunopurified sample to a mass spectrometry technique to obtain a mass spectrum of the samples;
identifying the presence of AIM polypeptides in said samples based on the multiply charged ion peaks in the spectrum corresponding to the AIM polypeptides;
converting the peak areas of the identified peaks to molecular masses to quantify the AIM polypeptides in said samples; and
comparing the quantities of AIM polypeptides from the initial sample and the one or more secondary samples.