Patent ID: 11921108
Assignee: FRAUNHOFER GESELLSCHAFT ZUR FÖRDERUNG DER ANGEWANDTEN FORSCHUNG E.V.
Field: Measurement (Instruments)
Classification: CPC G | IPC G

Claim 0:
1. A method for determining an amount of functional albumin, the method comprising:
a) providing a test sample containing a defined amount of albumin of unknown binding capacity and a reference sample containing the same defined amount of albumin having a reference binding capacity;
b) incubating the test sample and reference sample with a defined amount of at least one albumin-binding marker M under conditions that allow the at least one albumin-binding marker M to bind to albumin (A) to form complexes of the at least one albumin-binding marker M and albumin (M:A);
c) removing the complexes (M:A) formed in step c);
d) detecting a presence or an amount of unbound marker M in the samples after removal of the complexes (M:A) through first and second test strips that allow for a determination of an amount of unbound marker M, wherein the first and the second test strips have different detection limits/tolerance limits for the marker M, wherein said detecting is qualitative or semiquantitative detecting in which the unbound marker is detected based on the detection limit of the test strip; and
e) determining the amount of functional albumin based on the presence or the amounts detected of marker M in each of the first and second test strips in step d).