Patent ID: 11879162
Assignee: CALIFORNIA INSTITUTE OF TECHNOLOGY
Field: Biotechnology (Chemistry)
Classification: CPC C  Y | IPC C

Claim 0:
1. A non-transitory computer-readable storage medium storing instructions that, when executed by a processor, cause a system to perform operations comprising:
a) measuring, in a sample comprising a first primer with a fluorophore attached thereto and a second primer with a quencher attached thereto, a first signal generated by the fluorophore and the quencher, wherein the first primer and the second primer are specific for a first polynucleotide analyte in the sample, and the fluorophore is different from the quencher;
b) measuring a second signal generated by the fluorophore and the quencher from performing one or more first polymerization reactions with the first primer and the second primer using the first polynucleotide analyte as a template, wherein the one or more first polymerization reactions are performed after measuring the first signal;
c) comparing the first signal and the second signal to determine a first change in signal; and
d) determining a presence or absence of at least one genetic variation in the first polynucleotide analyte by comparing the first change in signal to a second change in signal obtained by performing operations a) through c) for a second polynucleotide analyte without the at least one genetic variation and corresponding to the first polynucleotide analyte.