Patent ID: 11952602
Assignee: SANOFI-AVENTIS DEUTSCHLAND GMBH
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 0:
1. A method for the production of human insulin, an insulin analogue or a derivative of insulin, the method comprising using a variant of porcine trypsin, wherein the variant of porcine trypsin comprises:
an amino acid sequence that has at least 91% sequence identity to SEQ ID NO: 1,
wherein said amino acid sequence differs from SEQ ID NO: 1 at least by one or more amino acid substitutions at one or more positions corresponding to F24, S44, D56, G78, Y131, S172 and W193 of native porcine trypsin according to SEQ ID NO: 1,
with the proviso that said amino acid sequence is not native porcine trypsin according to SEQ ID NO: 1; and
with the proviso that said amino acid sequence is not porcine variant trypsin S172A according to SEQ ID NO: 2.