Patent ID: 11906431
Assignee: NIKON CORPORATION
Field: Measurement (Instruments)
Classification: CPC G  H | IPC G  H

Claim 0:
1. A microscope apparatus comprising:
an illumination optical system that radiates activation light to activate some of fluorescent materials included in a sample and excitation light to excite at least some of the activated fluorescent materials;
an image forming optical system that has an objective lens and an astigmatic optical system, which generates astigmatism to at least part of fluorescence from the fluorescent materials, and that forms an image of the fluorescence;
an image-capturing unit that captures an image formed by the image forming optical system;
a drive unit that moves an image-capturing position in the sample along an optical axis-direction of the objective lens; and
a control unit, wherein
the control unit causes the image-capturing unit to capture images in a plurality of numbers of frames respectively at a first image-capturing position and at a second image-capturing position different from the first image-capturing position along the optical axis-direction,
the control unit causes the image-capturing unit to capture images in a first number of frames at the first image-capturing position and capture images in a second number of frames different from the first number of frames at the second image-capturing position, and
the first number of frames and the second number of frames are identified by image-capturing conditions set before the control unit causes the image-capturing unit to capture images at the first and second image-capturing positions.