Patent ID: 11873531
Assignee: ALCEDIAG
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 13:
14. The method according to claim 1, wherein the editing profile of the PDE8A pre-mRNA is determined by a process comprising performing a reverse transcription reaction on the cellular RNA extract and performing a nested type PCR comprising two rounds of PCR on the product of the reverse transcription, and
wherein:
a) the first round of PCR is carried out by the following sets of primers:
Forward:
PDE8A-1FWD (SEQ ID NO. 13) GCTGAAGCCTTCCTTCTAAGG,
Reverse:
PDE8A-1REV (SEQ ID NO. 12) GGACCTAGAGTTGACCCAGG, and
and wherein
b) the second round of PCR is carried out by the following set of primers:
Forward:
PDE 8 A-2Fwd FAM (SEQ ID NO. 10) CTAGGGAACCCTGTTTAGTCC,
Reverse:
PDE8A-2Rev VIC (SEQ ID NO. 11) CAATGGGCACCAAAAAAGGG.