Patent ID: 11891647
Assignee: NATIVE MICROBIALS, INC.
Field: Computer technology (Electrical engineering)
Classification: CPC C  G | IPC C  G

Claim 20:
21. A method of forming a bioreactive modificator based on microorganism strain absolute cell count analysis, the bioreactive modificator configured to alter a property in a target biological environment, comprising:
obtaining at least two sample sets, each set having a plurality of samples;
for an identified sample from a plurality of samples from a sample set, determining a presence of and total quantity of a plurality of microorganism strains;
identifying, by sequencing DNA in the identified sample a set of DNA representing whole genomes of the plurality of microorganism strains;
obtaining a set of sequencing reads associated with the plurality of microorganism strains from sequencing the DNA in the identified sample;
comparing each sequencing read from the set of sequencing reads with each DNA representing a whole genome in the set of DNA representing whole genomes to identify a match between that sequencing read and a DNA representing a whole genome;
determining, based on the comparing, a relative frequency of occurrence for each microorganism strain within the identified sample;
determining the absolute cell count of each microorganism strain in the identified sample from the total quantity of the plurality of microorganism strains and the relative frequency for each microorganism strain;
measuring RNA for an identified microorganism strain by sequencing cDNA in the identified sample and mapping the sequenced cDNA to the DNA representing a whole genome of the identified microorganism strain;
determining an activity for the identified microorganism strain in the identified sample based on the RNA for the identified microorganism strain;
identifying active microorganism strains in the identified sample, based on the activity for each microorganism strain in the identified sample and a measured metadata;
assigning each strain into one of at least two groups according to predicted function and/or chemistry;
for each group, identifying genetic similarities between at least two active microorganism strains in the group to identify at least one functionality of interest;
identifying one or more pathways based on the at least one identified functionality for each group;
selecting at least one compound based on the identified at least one functionality of interest; and
combining the selected compound with a carrier medium to form a bioreactive modificator configured to alter a property associated with the at least one metadata of a target biological environment when the bioreactive modificator is introduced into that target biological environment.