Patent ID: 11932897
Assignee: UNIVERSITY OF KENTUCKY RESEARCH FOUNDATION
Field: Pharmaceuticals (Chemistry)
Classification: CPC C  A | IPC A  C

Claim 17:
18. A method of making a curcuminoid in a mammalian cell, the method comprising:
introducing ferulic acid to the mammalian cell, and performing one of the following:
(a) expressing 4-coumaroyl-CoA ligase (4CL1) and curcuminoid synthase (CUS) in the mammalian cell;
(b) expressing tyrosine ammonia lyase (TAL), 4CL1, and CUS in the mammalian cell;
(c) expressing TAL, 4CL1, CUS, 4-coumarate 3-hydroxylase (C3H), and caffeoyl-CoA 3-0-methyltransferase (CCoAOMT) in the mammalian cell; or
(d) expressing TAL, 4CL1, C3H, CCoAOMT, curcumin synthase (CURS 1), and diketide-CoA synthase (DCS) in the mammalian cell;
wherein the expressing the enzymes converts ferulic acid to the curcuminoid.