Patent ID: 11878991
Assignee: TRILINK BIOTECHNOLOGIES, LLC
Field: Pharmaceuticals (Chemistry)
Classification: CPC C  A | IPC A  C

Claim 0:
1. A method for synthesizing a fully templated RNA molecule comprising the steps of:
introducing an initiating capped oligonucleotide primer, or a salt thereof of the structure:, , wherein:
each of B1 through B10 is independently a natural, modified or unnatural nucleoside base;
M is 0 or 1;
L is 0 or 1;
q1 is 1 and each of q2 through q9 is independently 0 or 1;
R1 is H or methyl;
R2 and R3 are each OH;
each of X1 through X13 is independently O or S;
each of Y1 through Y13 is independently OH, SH, BH3, aryl, alkyl, O-alkyl or O-aryl;
each of Z0 through Z22 is independently O, S, NH, CH2, C(halogen)2 or CH(halogen); and
each of R4 through R12 is independently H, OH, O-methyl or a detectable marker into a mixture comprising a polynucleotide template and an RNA polymerase under conditions conducive to transcription by the RNA polymerase of the polynucleotide template, wherein B1 in the initiating capped oligonucleotide primer is complementary to the base at position +1 in the promoter sequence of the polynucleotide template; and
incubating said mixture for a time sufficient to allow for transcription of said template.