Patent ID: 11905324
Assignee: INNOVATIVE TECHNOLOGIES IN BIOLOGICAL SYSTEMS SL
Field: Measurement (Instruments)
Classification: CPC C  G | IPC C  G

Claim 0:
1. A fluorescent fusion polypeptide capable of changing its localization and fluorescence intensity within a cell from the cell cytoplasmic membrane to the retention vesicles, upon an increase in translocation of beta-arrestin from the cell cytoplasm to the cell cytoplasmic membrane, comprising a membrane localization peptide consisting of the extracellular domain of interleukin-2 receptor of SEQ ID NO: 3; a peptide capable of binding G-protein or tyrosine receptors following phosphorylation of these receptors by GRKs or RTKs consisting of the phosphorylated receptor binding peptide from b-arrestin of SEQ ID NO: 2; a vesicularization peptide consisting of the Clathrin and beta-adaptin peptide from arrestin; and a fluorescent peptide wherein:
a. the membrane localization peptide is located at the N-terminus of the fluorescent fusion polypeptide and is physically bound, through a linker, to the fluorescent peptide, which in turn is physically bound, through a linker, to the peptide capable of binding G-protein or tyrosine receptors following phosphorylation of these receptors by GRKs or RTKs; and
b. the peptide capable of binding G-protein or tyrosine receptors following phosphorylation of these receptors by GRKs or RTKs is physically bound, through a linker, to the vesicularization peptide, which in turn is located at the C-terminus of the fluorescent fusion polypeptide;
and wherein the term “membrane localization peptide” means a peptide whose natural intracellular localization is in the plasma membrane.