Patent ID: 11898154
Assignee: SELEXIS S.A.
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 0:
1. A recombinant nucleic acid molecule that is part of a transposon vector comprising:
a) a 5′ transposon-specific inverted terminal repeat (ITR) and a 3′ ITR, and
b) located between the 5′ ITR and the 3′ ITR:
(i) at least one nucleic acid sequence encoding a transgene expression processing (TEP) protein under the control of a promoter,
wherein the TEP is selected from the group consisting of human Signal Recognition Particle 14 (hSRP14), human Protein Transport Protein 61α1 (hSec61α1), human Protein Transport Protein 61β (hSec61β), human Protein Transport Protein 61γ (hSec61γ), human Signal Recognition Particle 54 (hSRP54), human Signal Recognition Particle 9 (hSRP9), human Signal Recognition Particle Receptor α (hSRPRα), human Signal Recognition Particle β (hSRPβ), human Calnexin (hCANX), and combinations thereof, and
(ii) a matrix attachment region (MAR) element,, wherein the transposon vector increases an expression of a transgene encoding a product of interest in a mammalian cell by at least 10% relative to a plasmid vector comprising the TEP protein.