Patent ID: 11925174
Assignee: MERUS N.V.
Field: Measurement (Instruments)
Classification: CPC C  A  G | IPC A  C  G

Claim 0:
1. A transgenic murine animal that has been immunized to generate an immune response against an antigen, wherein the genome of said animal comprises a transgene comprising a single human immunoglobulin light chain V gene segment fused to a single human immunoglobulin light chain J gene segment,
such that said fused V/J gene segments encode a rearranged immunoglobulin light chain variable region, wherein the transgene lacks a regulatory element that contributes to somatic hypermutation of the light chain variable region; and
said transgenic animal comprising a population of B cells that secrete a repertoire of antibodies that bind said antigen, said repertoire of antibodies comprising the immunoglobulin light chain variable region encoded by said transgene and a light chain constant region, paired with a diversity of immunoglobulin heavy chains resulting from somatic hypermutation in response to said antigen.