Patent ID: 11865167
Assignee: UNIVERSITY OF PITTSBURGH—OF THE COMMONWEALTH SYSTEM OF HIGHER EDUCATION
Field: Pharmaceuticals (Chemistry)
Classification: CPC C  A | IPC A  C

Claim 0:
1. A method of treating a mammal having a cancer associated with expression of EGFRvIII comprising administering to the mammal an effective amount of cells expressing a chimeric antigen receptor (CAR) comprising a humanized anti-EGFRvIII binding domain, a transmembrane domain, and an intracellular signaling domain, wherein said anti-EGFRvIII binding domain comprises a light chain variable region and a heavy chain variable region, wherein:
(a) the heavy chain variable region comprises:
(i) a CDR1 comprising the sequence DYYIH (SEQ ID NO: 22);
(ii) a CDR2 comprising the sequence RIDPENDETKYGPIFQG (SEQ ID NO: 23); and
(iii) a CDR3 comprising the sequence RGGVY (SEQ ID NO: 24); and

(b) the light chain variable region comprises:
(i) a CDR1 comprising the sequence KSSQSLLDSDGKTYLN (SEQ ID NO: 26);
(ii) a CDR2 comprising the sequence LVSKLDS (SEQ ID NO: 27); and
(iii) a CDR3 comprising the sequence WQGTHFPGT (SEQ ID NO: 28).