Patent ID: 11965191
Assignee: CALIFORNIA INSTITUTE OF TECHNOLOGY
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 14:
15. The synthetic protein circuit of claim 13, wherein the second degron of the first target protein comprises a masking peptide of the second degron of the first target protein connected to the second degron of the first target protein, wherein the masking peptide of the second degron of the first target protein prevents the second degron of the first target protein from destabilizing the first target protein when the masking peptide of the second degron of the first target protein is present on the first target protein, wherein the masking peptide of the second degron of the first target protein is configured to be cleaved from the first target protein when the second cut site of the first target protein specific for the second protease is cleaved by the second protease, wherein the first target protein is configured to be destabilized by cleavage of the second cut site specific for the second protease, and wherein cleavage of the second cut site of the first target protein specific for the second protease uncovers the second degron of the first target protein thereby destabilizing the first target protein.