Patent ID: 11946043
Assignee: LUDWIG INSTITUTE FOR CANCER RESEARCH LTD
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 12:
13. A method for selectively sequencing 5mC containing DNA from a DNA sample comprising the steps in order of:
(a) adding a blocking group to the endogenous 5-hydroxymethylcytosine (5hmC) in the DNA sample;
(b) converting the 5mC in the DNA sample to 5hmC with Naegleria gruberi TET1 (NgTET1);
(c) adding a linking group to the 5hmC from step (b);
(d) after step (c), ligating adapter DNA to the DNA sample;
(e) after step (d), adding an affinity tag to the to the linking group from step (c);
(f) enriching for the affinity tagged DNA from step (e) by affinity purification utilizing a support;
(g) amplifying the enriched DNA from step (f), wherein amplifying the enriched DNA comprises PCR directly on the enriched DNA linked to the support; and
(h) sequencing the amplified DNA.