Patent ID: 11904259
Assignee: ROSETTA EXOSOME
Field: Chemical engineering (Chemistry)
Classification: CPC B  C  G | IPC B  C  G

Claim 0:
1. A method for isolating extracellular vesicles, the method comprising:
(a) adding cations to a biological sample containing extracellular vesicles at a concentration sufficient to form an extracellular vesicle-cation insoluble complex, and wherein the cations have a specific affinity for the extracellular vesicles, with the proviso that if the cations are calcium ions, then the calcium ions are added at a concentration of 10 mM or greater, wherein the cations exclude sodium, lithium, or potassium ions;
(b) inducing a specific binding between the extracellular vesicles and the cations by reacting the cations with the extracellular vesicles contained in the biological sample, thereby forming an extracellular vesicle-cation insoluble complex;
(c) separating the extracellular vesicle-cation insoluble complex from the biological sample, wherein the separating is performed by using at least one method selected from the group consisting of centrifugation, ultracentrifugation, filtration, ultrafiltration, gravity, density gradient ultracentrifugation, precipitation, polymer-based precipitation, and organic solvent precipitation; and
(d) separating the cations from the complex to purify the extracellular vesicles.