Patent ID: 11898211
Assignee: LAWRENCE LIVERMORE NATIONAL SECURITY, LLC
Field: Biotechnology (Chemistry)
Classification: CPC C  G | IPC C  G

Claim 18:
19. The UO2F2 biosensor of claim 15, wherein
the cell is further natively and/or heterologously expressing histidine kinase UzcS, and response regulator UzcS, and
the U-sensing genetic circuit further comprises a UzcR U-sensing genetic reportable molecular component and/or a UzcR U-sensing/U-neutralizing genetic molecular component, each comprising a UzcR U-sensitive promoter in a configuration wherein the U-sensitive promoter directly initiates expression of the U-sensing reportable molecular component and/or of the U-sensing U-neutralizing molecular component in presence of bioavailable U,, the UzcR U sensitive promoter comprising a UzcR binding site having DNA sequence:, (SEQ ID NO: 2)

CATTACN7N8N9N10N11N12TTAA

wherein N7-N12 is independently any nucleotide, and in some embodiments any one of N7-N11 can independently be A.