Patent ID: 11859257
Assignee: GEN-PROBE INCORPORATED
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 11:
12. The composition of claim 1, wherein the composition comprises at least one pair of S. aureus-specific or S. aureus-indicative amplification oligomers configured to produce an S. aureus-specific or S. aureus-indicative amplicon, wherein:
(i) the pair of S. aureus-specific or S. aureus-indicative amplification oligomers is configured to hybridize specifically to one of nuc, rRNA, femB, Sa442, or Staphyloxanthin, in an S. aureus chromosome;
(ii) at least one S. aureus-specific or S. aureus-indicative amplification oligomer competes for binding to S. aureus GAPDH under stringent conditions with an oligomer having a sequence consisting of SEQ ID NO: 20 or 23 or at least one S. aureus-specific or S. aureus-indicative amplification oligomer comprises the sequence of SEQ ID NO: 20 or 23; or
(iii) at least one S. aureus-specific or S. aureus-indicative amplification oligomer competes for binding to S. aureus GAPDH under stringent conditions with an oligomer having a sequence consisting of SEQ ID NO: 21, 24, or 26 or comprises the sequence of SEQ ID NO: 21, 24, or 26;
optionally wherein the composition comprises at least one S. aureus-specific or S. aureus-indicative primary detection oligomer, further optionally wherein the S. aureus-specific or S. aureus-indicative primary detection oligomer competes for binding to S. aureus GAPDH under stringent conditions with an oligomer having a sequence consisting of SEQ ID NO: 22, 25, 114, or 118 or the S. aureus-specific or S. aureus-indicative primary detection oligomer comprises the sequence of at least one of SEQ ID NO: 95, 96, 107, 108, 22, 25, 114, or 118.