Patent ID: 11926865
Assignee: THE BOARD OF TRUSTEES OF THE LELAND STANFORD JUNIOR UNIVERSITY
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 0:
1. A composition comprising:
(a) a plurality of capture agents, wherein the plurality of capture agents can specifically bind to different complementary sites in a biological sample,
wherein each of the plurality of capture agents comprises a double stranded nucleic acid, and wherein a 3′ end of a first strand of the double stranded nucleic acid is recessed relative to a 5′ end of a second strand of the double stranded nucleic acid, thereby producing a 5′ overhang;
(b) a polymerase;
(c) a plurality of nucleotides;
wherein the polymerase is configured to extend a strand of the double stranded nucleic acid with the plurality of nucleotides; and
wherein:
(i) the plurality of nucleotides comprise a fluorescent nucleotide and/or
(ii) the composition further comprises a fluorescently labeled oligonucleotide complementary to a sequence of an overhang of at least one capture agent of the plurality of capture agents.