Patent ID: 11898198
Assignee: ILLUMINA, INC.
Field: Computer technology (Electrical engineering)
Classification: CPC C  G | IPC C  G

Claim 0:
1. A set of sequencing adapters comprising a plurality of double-stranded polynucleotides, wherein:
each double-stranded polynucleotide comprises a double-stranded hybridized region, a single-stranded 5′ arm, a single-stranded 3′ arm, and at least one variable-length, nonrandom unique molecular index (vNRUMI);
variable-length, nonrandom unique molecular indices (vNRUMIs) of the set of sequencing adapters form a set of vNRUMIs configured to identify individual nucleic acid molecules in a sample for multiplex massively parallel sequencing;
the set of vNRUMIs comprises sequences having two or more molecular lengths;
an edit distance between any two vNRUMIs of the set of vNRUMIs is not less than a first criterion value, wherein the first criterion value is at least two; and
edit distances among the vNRUMIs in the set of vNRUMIs are maximized by a greedy approach.