Patent ID: 11952611
Assignee: LABORATORY CORPORATION OF AMERICA HOLDINGS
Field: Measurement (Instruments)
Classification: CPC C  G | IPC C  G

Claim 18:
19. A kit for detecting antibiotic-resistant bacteria in a sample, wherein the kit comprises:
(a) a recombinant bacteriophage specific to the bacteria, wherein the recombinant bacteriophage comprises a genetic construct inserted into a late gene region of the bacteriophage genome, the genetic construct comprising:
(i) an indicator gene, wherein the indicator gene is not contiguous with a gene encoding a structural bacteriophage protein and does not yield a fusion protein; and
(ii) a promoter controlling transcription of the indicator gene, wherein the promoter is a bacteriophage late promoter, and wherein expression of the indicator gene results in an indicator protein product, and wherein the indicator protein product is a luciferase; and

(b) an antibiotic.