Patent ID: 11971403
Assignee: OIL CROPS RESEARCH INSTITUTE, CHINESE ACADEMY OF AGRICULTURAL SCIENCES
Field: Measurement (Instruments)
Classification: CPC G | IPC G

Claim 6:
7. The time-resolved fluorescence kit according to claim 1, wherein a preparation method for the immunochromatography time-resolved fluorescence test strip comprises the following steps:
(1) preparation of the water absorption pad by cutting water absorption paper;
(2) preparation of the detection pad:
preparing coating solutions with a concentration of 0.25 mg/mL to 2 mg/mL from the 4,15-diacetoxyscirpenol-bovine serum albumin conjugate, the aflatoxin B1-bovine serum albumin conjugate, and the sterigmatocystin-bovine serum albumin conjugate respectively, coating the nitrocellulose membrane with the coating solutions at intervals in a streak manner to obtain the three detection lines, respectively, and drying at 37° C. to 40° C. for 30 min to 60 min;
wherein in the test strip, a distance between the detection line close to the quality control line and an upper edge of the nitrocellulose membrane is 15 mm to 20 mm, a distance between two adjacent detection lines is 1.0 mm to 5.5 mm, and a distance between the detection line close to the quality control line and the quality control line is 5 mm to 10 mm; and
preparing a rabbit antimouse polyclonal antibody coating solution with a concentration of 0.1 mg/mL to 0.85 mg/mL, and transversely coating the nitrocellulose membrane with the coating solutions in a position 5 mm to 10 mm away from the detection lines in a streak manner to obtain the quality control line, wherein a coating quantity of the rabbit antimouse polyclonal antibody required for each centimeter of the quality control line is 0.4 μg to 0.8 μg; and drying at 37° C. to 40° C. for 30 min to 60 min;
(3) preparation of the sample pad:
putting a fiberglass membrane into a blocking solution to be soaked, taking out the soaked fiberglass membrane, and drying at 37° C. to 40° C. for 4 h to 10 h to obtain the sample pad, and finally preserving the sample pad in a dryer at room temperature; and
(4) assembling of the immunochromatography time-resolved fluorescence test strip:
sequentially attaching the water absorption pad, the detection pad and the sample pad to one side of a paperboard from top to bottom to obtain the immunochromatography time-resolved fluorescence test strip, wherein adjacent pads are connected in an overlapping manner at a joint based on an overlapping length of 1 mm to 3 mm.