Patent ID: 11867687
Assignee: DANA-FARBER CANCER INSTITUTE, INC.
Field: Measurement (Instruments)
Classification: CPC G | IPC G

Claim 0:
1. A method of determining responsiveness of a cancer cell population to a chemotherapeutic agent, the method comprising:
a) permeabilizing cells from a cancer cell population;
b) contacting the permeabilized cells with a Bcl-2 homology 3 (BH3) domain peptide;
c) fixing the permeabilized cells contacted with the BH3 domain peptide;
d) staining the fixed cells for a molecule from the mitochondrial intermembrane space; and
e) detecting the staining for the molecule from the mitochondrial intermembrane space to measure an amount of the molecule in the stained fixed cells,
wherein a decrease in the amount of the molecule measured in the cells compared to an amount of the molecule measured in permeabilized cells not contacted with the BH3 domain peptide indicates that the cancer cell population is responsive to treatment with a chemotherapeutic agent.