Patent ID: 11867893
Assignee: DISRUPTIVE TECHNOLOGICAL ADVANCES IN LIFE SCIENCE S.R.L.
Field: Measurement (Instruments)
Classification: CPC G | IPC G

Claim 1:
2. The fluorescence microscopy method according to claim 1, wherein step D comprises, for each one of the M target pixels PN, the following substeps:
D.1—setting the first initial configuration C1 of the wavefront as a reference configuration C1=Cref of the wavefront, the first initial image F1 as a reference image F1=Fref, and a first intensity IPN1 at the target pixel PN of the first initial image F1 as a reference intensity IPN1=IPNref at the target pixel PN;
D.2—repeating T times, with T>1, the following cycle of substeps:
D.2.1—varying the reference configuration Cref of the wavefront through an amplitude and/or phase modulation unit so as to obtain an i-th configuration Ci of the wavefront;
D.2.2—acquiring an i-th image Fi with an i-th intensity IPNi at the target pixel PN;
D.2.3—checking whether the i-th intensity IPNi value at the target pixel PN is greater than the reference intensity IPNref thereby:
if IPNi>IPNref, then the i-th configuration Ci of the wavefront obtained at step D.2.1 is accepted as the reference configuration Cref of the wavefront, i.e. Cref=Ci, and Fref=Fi and IPNref=IPNi are set, else
if IPNi<IPNref, then the i-th configuration Ci of the wavefront obtained at step D.2.1 is not accepted, and the reference configuration Cref of the wavefront before the variation performed at step D.2.1 is restored;

D.3—setting the reference image Fref as the final image Fref=Ffin(IPNmax) and the reference intensity IPNref as a local maximum IPNmax of florescence signal at the target pixel PN.