Patent ID: 11858958
Assignee: SHANGHAI GINSOME PHARMATECH CO., LTD.
Field: Pharmaceuticals (Chemistry)
Classification: CPC A  C | IPC A  C

Claim 23:
24. A process for preparing the active substance-loaded liposome of claim 21, comprising:
step (1): mixing the lipid, the ginsenoside of Formula I and the active substance in an organic solvent to obtain a clear solution, optionally, with one or more agents selected from a cholesterol, a long-circulating material, a hydrophobic antioxidant, a soybean oil, sodium oleate, a hydrophobic surfactant, a hydrophobic heat-sensitive excipient, a hydrophobic pH sensitive material, and a hydrophobic ionic additive; wherein the solvent is one or more solvents selected from alcohols, halogenated hydrocarbon solvents and nitrile solvents; wherein the ginsenoside of Formula I is micronized into ultrafine powder and the average particle size is no more than 50 μm; or no more than 20 μm, or no more than 10 μm; and
step (2): removing the organic solvent from the clear solution obtained in step (1) to form a film, mixing the film with an aqueous solution comprising a cryoprotectant, and optionally one or more agents selected from a hydrophilic antioxidant, a hydrophilic surfactant, a hydrophilic heat-sensitive excipient, a hydrophilic pH sensitive material, and a hydrophilic ionic additive to give a mixture; performing an operation of sonification or high pressure homogenization, filtering the mixture to obtain an aqueous solution containing the active substance-loaded liposome, drying the aqueous solution to obtain the active substance-loaded liposome of claim 21.