Patent ID: 11898196
Assignee: QUANTUM-SI INCORPORATED
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 0:
1. A method for separating target heteroduplex nucleic acid from background nucleic acid, comprising;
nicking the target heteroduplex nucleic acid;
loading a mixture of nicked target heteroduplex nucleic acid and background homoduplex nucleic acid on a separation medium, wherein the target heteroduplex nucleic acid and the background homoduplex nucleic acid differ by at least one base;
contacting the mixture of target heteroduplex nucleic acid and background homoduplex nucleic acid with a heteroduplex-binding protein to create a heteroduplex-binding protein-nucleic acid mixture;
applying a time-varying driving field to the separation medium; and
applying a time-varying mobility varying field to the separation medium,
thereby causing the target heteroduplex nucleic acid to be separated from the background homoduplex nucleic acid.