Patent ID: 11965877
Assignee: BIO-RAD LABORATORIES, INC.
Field: Measurement (Instruments)
Classification: CPC G  C | IPC C  G

Claim 0:
1. A method for barcoding RNA from a single cell, the method comprising:
forming a plurality of aqueous droplets in oil, each droplet comprising a plurality of different RNA molecules from a single cell and a plurality of nucleic acid constructs not attached to microbeads, wherein each of the nucleic acid constructs comprises a universal priming site, a barcode sequence and a poly(dT) sequence, wherein the barcode sequence is a unique barcode that allows sequence reads to be assigned to one molecule from a sample;
hybridizing at least one nucleic acid construct to at least one RNA molecule in at least one of the droplets to form at least one hybridized nucleic acid construct that is not attached to a microbead;
extending the at least one hybridized nucleic acid construct that is not attached to the microbead to form an extension product that is not attached to a microbead; and
amplifying, using the universal priming site, the extension product to yield an amplified product.