Patent ID: 11965191
Assignee: CALIFORNIA INSTITUTE OF TECHNOLOGY
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 15:
16. The synthetic protein circuit of claim 13, wherein the second degron of the second target protein comprises a masking peptide of the second degron of the second target protein connected to the second degron of the second target protein, wherein the masking peptide of the second degron of the second target protein prevents the second degron of the second target protein from destabilizing the second target protein when the masking peptide of the second degron of the second target protein is present on the second target protein, wherein the masking peptide of the second degron of the second target protein is configured to be cleaved from the second target protein when the second cut site of the second target protein specific for the first protease is cleaved by the first protease, wherein the second target protein is configured to be destabilized by cleavage of the second cut site of the second target protein specific for the first protease, and wherein cleavage of the second cut site of the second target protein specific for the first protease uncovers the second degron of the second target protein thereby destabilizing the second target protein.