Patent ID: 11898200
Assignee: HUAQIAO UNIVERSITY
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 7:
8. The method for detecting SSBs in DNA of claim 1, wherein revealing the positions of the SSBs comprises:
(1) selecting paired-end reads, where a first read starts with a corresponding number of complementary bases of the second tail and a second read starts with a corresponding number of complementary bases of the first tail,
(2) aligning the paired-end reads after bases corresponding to PCR primers have been removed to assemblies of specified genomes, wherein only pairs of reads where the first read and the second read uniquely mapped to a genome of the specified genomes with preset configuration and spacing are kept, and
(3) removing pairs of reads where a fraction of T's in a 20-base sequence of 5′ upstream of an alignment of the second read of the paired-end reads is >40%, and defining an SSB as a first base in the second read of remaining read pairs of the paired-end reads.