Patent ID: 11866702
Assignee: UNIVERSITY OF ROCHESTER
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 0:
1. A nucleic acid molecule comprising a guide region consisting of a single hairpin structure corresponding to one of the two hairpin structures of the wild type H/ACA snoRNA, wherein
(i) the nucleic acid molecule is capable of forming a partially double stranded nucleic acid complex with a target RNA in a mammalian cell, wherein the target RNA comprises a target uridine;
(ii) the partially double stranded nucleic acid complex is capable of engaging a mammalian pseudouridylation enzyme;
(iii) the guide region assists in positioning the target uridine in the partially double stranded nucleic acid complex; and
(iv) the target uridine can be converted to a pseudouridine by the mammalian pseudouridylation enzyme.