Patent ID: 11859211
Assignee: PRESIDENT AND FELLOWS OF HARVARD COLLEGE
Field: Pharmaceuticals (Chemistry)
Classification: CPC C  A | IPC A  C

Claim 29:
30. A method for treatment of a muscle disorder in a subject in need thereof, the method comprising administering to the subject a pharmaceutical composition comprising therapeutically effective amount of an isolated population of non-naturally occurring satellite cells produced according to a method comprising:
contacting a population of cells comprising myoblasts in spin culture with a first medium, wherein the first medium is a myoblast medium, to form an expanded myoblast population;
contacting the expanded myoblast population with a second medium, wherein the second medium is a spin medium, to form at least one skeletal muscle organoid comprising differentiated and proliferative cells; and
contacting the at least one skeletal muscle organoid in spin culture with a third medium, wherein the third medium is a differentiation medium, for a time sufficient to induce the dedifferentiation of at least one proliferative cell of the skeletal muscle organoid into a non-naturally occurring satellite cell,
wherein the non-naturally occurring satellite cells produced express two or more genes selected from the group consisting of Nr2f1, Dmpl, Wisp2, Ecm1, and Mgp, and
wherein the expression of the two or more genes is upregulated by greater than 2-fold relative to expression by myoblasts and the two or more genes are not expressed in an endogenous satellite cell.