Patent ID: 11913003
Assignee: BEIJING DABEINONG BIOTECHNOLOGY CO., LTD.
Field: Biotechnology (Chemistry)
Classification: CPC C | IPC C

Claim 0:
1. A method for producing a transformed soybean plant, which comprises:
(a) excising the meristem from a soybean plant to produce a meristem block;
(b) introducing a recombinant vector containing a gene of interest and a gene encoding a sulfonylurea herbicide hydrolase to the meristem block, wherein the sulfonylurea herbicide hydrolase comprises: (a) a protein composed of an amino acid sequence as shown in SEQ ID NO: 2; or (b) a protein derived from the protein in (a) by substitution and/or deletion and/or addition of one or several amino acids in the amino acid sequence as shown in SEQ ID NO: 2 and having an aryloxyalkanoate dioxygenase activity; or (c) a protein composed of an amino acid sequence having at least 90% sequence identity to SEQ ID NO: 2;
(c) placing the meristem block on a medium;
(d) spraying a tribenuron methyl on the meristem block on the proliferation medium to select for transformed resistant tissue and 1-7 μg of tribenuron methyl is externally applied to per ml of the proliferation medium, and
(e) regenerating the transformed resistant tissue into a soybean plant, wherein the regeneration is done by placing the transformed resistant tissue on a medium and spraying a tribenuron methyl on the transformed resistant tissue,
wherein the transformation rate of the method is higher than the transformation rate obtained from a method in which the tribenuron methyl is added to the medium prior to placing the meristem block on the medium.