Patent ID: 11874280
Assignee: SONY GROUP CORPORATION
Field: Measurement (Instruments)
Classification: CPC G | IPC G

Claim 3:
4. A method for detecting a target analyte, the method comprising:
a) associating a covalent conjugate with the target analyte to form an analyte-targeting moiety complex, the covalent conjugate comprising:
i) a targeting moiety that has affinity for the target analyte and comprises a covalent bond to a polymeric dye; and
ii) the polymeric dye comprising:
A) two or more fluorescent or colored moieties;
B) at least one negatively charged phosphate group; and
C) a covalent bond to the targeting moiety,

wherein each of the two or more fluorescent or colored moieties are joined to an adjacent fluorescent or colored moiety via a linker comprising the at least one negatively charged phosphate group;

b) treating:
i) the covalent conjugate and the target analyte with a magnesium salt during the associating the covalent conjugate and the target analyte; and/or
ii) the analyte-targeting moiety complex with a wash solution comprising a magnesium salt after the associating the covalent conjugate and the target analyte; and

c) detecting a fluorescent or colored signal from the analyte-targeting moiety complex.