Patent ID: 11955241
Assignee: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA
Field: Measurement (Instruments)
Classification: CPC G  Y | IPC G

Claim 7:
8. A method comprising:
treating a subject having vascular anomalies or malformations selected from the group consisting of arterio-venous malformations (AVM), venous malformations (VM), port-wine stain (PWS), Sturge-Weber syndrome (SWS), Klippel-Trenaunay-Weber syndrome (KTWS), hemangioma, cavernoma, capillary telangiectasia, dural AV fistula, and angiokeratoma with one or more treatments for the vascular anomalies or malformations;
obtaining a skin tissue biopsy sample(s) and/or extracellular vesicle (EV) serum sample(s) from the subject at the site of the vascular anomaly or malformation;
measuring the expression profile of a set of pathogenic associated biomarkers and/or serum EV biomarkers from sample(s) obtained from the subject, wherein the set of pathogenic associated biomarkers and/or serum EV biomarkers comprises VAT1, IQGAP1, HSC70, CLH1, perlecan, spectrin α1, and GDIR1;
comparing the expression profile of the set pathogenic associated biomarkers and/or serum EV biomarkers from the subject's skin tissue biopsy sample(s) and/or EV serum sample(s) with the expression profile of the same set pathogenic associated biomarkers and/or serum EV biomarkers from control skin tissue biopsy sample(s) and/or EV serum sample(s) that do not have a vascular anomaly or malformation;
identifying that the treatment for vascular anomalies or malformations in the subject was effective based upon measuring an improvement in the expression levels of the set of pathogenic associated biomarkers and/or serum EV biomarkers in the subject's sample(s).