---
license: cc-by-nc-nd-4.0
extra_gated_fields:
  Name: text
  Company: text
  Country: country
  Specific date: date_picker
  I want to use this model for:
    type: select
    options: 
      - Research
      - Education
      - label: Other
        value: other
  I agree to include the authors of the code (Tianlai Chen and Pranam Chatterjee) as authors on manuscripts with data from designed peptides: checkbox
  I agree to share generated sequences and associated data with authors before publishing: checkbox
  I agree not to file patents on any sequences generated by this model: checkbox
  I agree to use this model for non-commercial use ONLY: checkbox
---
**PepMLM: Target Sequence-Conditioned Generation of Peptide Binders via Masked Language Modeling**
![image/png](https://cdn-uploads.huggingface.co/production/uploads/63df6223f351dc0745681f77/hkKA0GttGY5l3oVcKf0bR.png)
In this work, we introduce **PepMLM**, a purely target sequence-conditioned *de novo* generator of linear peptide binders. 
By employing a novel masking strategy that uniquely positions cognate peptide sequences at the terminus of target protein sequences, 
PepMLM tasks the state-of-the-art ESM-2 pLM to fully reconstruct the binder region, 
achieving low perplexities matching or improving upon previously-validated peptide-protein sequence pairs. 
After successful *in silico* benchmarking with AlphaFold-Multimer, we experimentally verify PepMLM’s efficacy via fusion of model-derived peptides to E3 ubiquitin ligase domains, demonstrating endogenous degradation of target substrates in cellular models. 
In total, PepMLM enables the generative design of candidate binders to any target protein, without the requirement of target structure, empowering downstream programmable proteome editing applications.     

- Demo: HuggingFace Space Demo [Link](https://huggingface.co/spaces/TianlaiChen/PepMLM).[Temporarily Unavailable]
- Colab Notebook: [Link](https://colab.research.google.com/drive/1u0i-LBog_lvQ5YRKs7QLKh_RtI-tV8qM?usp=sharing)
- Preprint: [Link](https://arxiv.org/abs/2310.03842)

# Apply for Access
As of February 2024, the model has been gated on HuggingFace. If you wish to use our model, please visit our page on the HuggingFace site ([Link](https://huggingface.co/ChatterjeeLab/PepMLM-650M)) and submit your access request there. An active HuggingFace account is necessary for both the application and subsequent modeling use. Approval of requests may take a few days, as we are a small lab with a manual approval process.

Once your request is approved, you will need your personal access token to begin using this notebook. We appreciate your understanding.

- How to find your access token: https://huggingface.co/docs/hub/en/security-tokens

```
# Load model directly
from transformers import AutoTokenizer, AutoModelForMaskedLM

tokenizer = AutoTokenizer.from_pretrained("TianlaiChen/PepMLM-650M")
model = AutoModelForMaskedLM.from_pretrained("TianlaiChen/PepMLM-650M")
```
![Logo](logo.png)